CN217377883U - Molecular diagnosis test card - Google Patents
Molecular diagnosis test card Download PDFInfo
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- CN217377883U CN217377883U CN202220233528.3U CN202220233528U CN217377883U CN 217377883 U CN217377883 U CN 217377883U CN 202220233528 U CN202220233528 U CN 202220233528U CN 217377883 U CN217377883 U CN 217377883U
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Abstract
The utility model belongs to the technical field of molecular diagnosis, especially, relate to a molecular diagnosis test card. It includes: the body and the sealing cover are arranged on the cover body on the front surface of the body; the body outside the sealing cover area of the cover body is provided with a sample adding groove, and the body in the sealing cover area of the cover body is provided with a flow groove, a waste liquid groove, a quantifying groove and a detection groove; the flow groove is communicated with the sample adding groove and is communicated to the quantifying groove through a first flow channel, the quantifying groove is communicated with the detection groove through a second flow channel, and the flow groove is communicated to the waste liquid groove through a third flow channel; the top of the first flow channel is lower than the bottom of the third flow channel, and the bottom of the second flow channel is higher than the bottom of the third flow channel; be equipped with mobilizable shutoff piece in the chute, the shutoff piece carries out the shutoff to first flow passage under centrifugal action. The utility model discloses can effectively avoid the test card too much the problem that enters into in the detection groove of the appearance liquid volume that awaits measuring that takes place in centrifuge working process, improve the detection precision and the detection effect of test card.
Description
Technical Field
The utility model belongs to the technical field of molecular diagnosis, especially, relate to a molecular diagnosis test card.
Background
Molecular diagnostics refers to techniques for making a diagnosis by detecting changes in the structure or expression level of genetic material in a patient using molecular biology methods. Molecular diagnosis is a main method for prediction diagnosis, and can be used for diagnosis of individual genetic diseases and prenatal diagnosis. Molecular diagnostics mainly refers to the detection of genes encoding various structural proteins, enzymes, antigens, antibodies, and immunologically active molecules associated with diseases.
The molecular diagnosis test card is a disposable instrument commonly used for matching with a centrifuge to perform molecular diagnosis, and the existing test card can basically meet the requirement of one card for multiple tests, namely, one sample to be tested is added into one test card, and the centrifuge is used for mixing the sample to be tested and a detection reagent, so that multiple tests can be realized. However, the existing detection card has certain defects, especially in the aspects of high false positive and false negative rate and the like. This is because current molecular diagnosis test card is though with each detection groove independent setting and the corresponding ration groove that has set up, can realize preliminary ration sample through the ration groove, but when carrying out centrifugal test in the centrifuge of reality, actually can have the appearance that awaits measuring in the partial flow groove to enter into the detection groove through the ration groove once more, leads to getting into the decline of the appearance liquid volume accuracy that awaits measuring in the detection groove, finally leads to the testing result to appear the deviation easily.
Disclosure of Invention
For solving current molecular diagnosis test card and leading to the testing result precision to change owing to the appearance liquid volume of awaiting measuring that detects in the test groove in the in-service use process easily to part test card needs the strict control application of sample speed to lead to detection efficiency low scheduling problem, the utility model provides a molecular diagnosis test card.
The utility model aims to provide a:
firstly, the use and detection efficiency of the molecular diagnosis test card is improved;
and secondly, the liquid amount of the sample to be detected in the detection tank can be prevented from generating obvious fluctuation to influence the detection result.
In order to achieve the above purpose, the utility model adopts the following technical scheme.
A molecular diagnostic test card, comprising:
the body and the sealing cover are arranged on the cover body on the front surface of the body;
the body outside the sealing cover area of the cover body is provided with a sample adding groove, and the body in the sealing cover area of the cover body is provided with a flow groove, a waste liquid groove, a quantifying groove and a detection groove;
the flow groove is communicated with the sample adding groove and is communicated to the quantitative groove through a first flow passage, the quantitative groove is communicated with the detection groove through a second flow passage, and the flow groove is communicated to the waste liquid groove through a third flow passage;
the top of the first flow channel is lower than the bottom of the third flow channel, and the bottom of the second flow channel is higher than the bottom of the third flow channel;
be equipped with mobilizable shutoff piece in the chute, the shutoff piece carries out the shutoff to first flow passage under centrifugal action.
As a matter of preference,
the flow groove is provided with a guide rod pointing to the direction of the quantitative groove and the direction of the detection groove;
the blocking piece is connected with the guide rod in a sliding mode and is provided with a blocking head matched with the first flow channel, and the blocking piece moves along the guide rod to close the first flow channel to isolate the quantitative groove and the flow groove.
Preferably, the plugging piece comprises a rod portion and a head portion, the head portion faces the quantifying groove and the detecting groove, the rod portion is U-shaped, a clamping block is arranged on the inner side of the rod portion, the guide rod is provided with a sliding groove, the plugging piece and the guide rod are connected in a sliding mode through the clamping block and the sliding groove, and the plugging head is arranged on the other side, back to the rod portion, of the head portion of the plugging piece.
As a matter of preference,
an elastic sealing body is arranged in the second flow channel.
As a preference, the first and second liquid crystal compositions are,
the elastic sealing body comprises an elastic body seat, a sealing ball and a limiting net;
the projectile body seat is funnel-shaped and is fixedly connected with the second flow channel wall, the tip end of the projectile body seat is provided with a mouth part and faces the quantitative groove, and the limiting net is fixedly connected with the second flow channel wall and tightly presses the sealing ball in the projectile body seat.
As a preference, the first and second liquid crystal compositions are,
the limiting net is an elastic fiber net.
As a preference, the first and second liquid crystal compositions are,
the outer diameter of the sealing ball is larger than the inner diameter of the mouth.
The utility model has the advantages that:
1) the use efficiency of the test card can be greatly improved, the problem that the existing test card is easy to overflow a detection groove when being quickly added with sample can be solved, and meanwhile, the problem that the existing test card is easy to cause the leakage of a detection reagent to the outside of the detection groove or the too early and too much sample to be detected to enter the detection groove under the condition of inclined placement can be avoided;
2) the problem that the liquid amount of a sample to be tested, which occurs in the working process of the centrifugal machine, of the test card excessively enters the detection groove can be effectively avoided, and the detection precision and the detection effect of the test card are improved.
Drawings
Fig. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the structure of the utility model;
FIG. 3 is a schematic view of the construction of the block piece;
FIG. 4 is an enlarged, semi-sectional view of portion A of FIG. 2;
FIG. 5 is a schematic view of the cooperation between each trough and the flow channel of the present invention;
FIG. 6 is a dynamic schematic view of the sealing elastomer of the present invention;
in the figure: 10 body, 20 lid, 100 application of sample groove, 200 chute, 201 guide bar, 2011 spout, 202 shutoff piece, 2021 pole portion, 2022 head, 2023 shutoff head, 2024 fixture block, 300 waste liquid groove, 400 quantitative groove, 500 detection grooves, 600 first flow path, 700 second flow path, 800 third flow path, 900 elastic sealing body, 901 elastomer seat, 902 ball sealer, 903 limiting net.
Detailed Description
The invention is described in further detail below with reference to specific embodiments and drawings. Those of ordinary skill in the art will be able to implement the invention based on these descriptions. Furthermore, the embodiments of the invention described in the following description are generally only some embodiments, but not all embodiments of the invention. Therefore, all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "thickness", "upper", "lower", "horizontal", "top", "bottom", "inner", "outer", "circumferential", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience of description and simplification of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., and "a plurality" means one or more unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; may be mechanically coupled, may be electrically coupled or may be in communication with each other; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
Examples
A molecular diagnostic test card as shown in fig. 1 and 2, which specifically comprises:
the body 10 and the transparent visual cover body 20 with the sealing cover arranged on the front surface of the body 10 are provided, and the cover body 20 is made of transparent conventional plastic materials;
the front surface of the body 10 is provided with a sample adding groove for dropwise adding a sample to be detected and a flow groove 100 for preliminarily containing the sample to be detected, the sample adding groove is communicated with the flow groove 100, and the sample to be detected is dropwise added into the flow groove 100 through the sample adding groove;
a waste liquid tank 300, a plurality of quantitative tanks 400 and detection tanks 500 with the same number as the quantitative tanks 400 are also arranged in the area of the front surface of the body 10 sealed by the cover body 20;
reagents for detection are respectively arranged in the detection tanks 500;
the flow groove 100 is communicated with the quantification grooves 400 through a first flow passage 600, the quantification grooves 400 are communicated with the detection grooves 500 through a second flow passage 700, the flow groove 100 is also communicated with the waste liquid groove 300 through a third flow passage 800, each quantification groove 400 is communicated with one detection groove 500 correspondingly, the quantification grooves 400 are mutually independent and not communicated, and the detection grooves 500 are mutually independent and not communicated;
the top of the first flow channel 600 is lower than the bottom of the third flow channel 800, the bottom of the second flow channel 700 is higher than the bottom of the third flow channel 800, and the bottom of the first flow channel 600 is higher than the bottom of the runner 100 by at least 3mm, and the schematic view of each flow channel and the runner is shown in fig. 5;
the flow cell 100 is internally provided with a plugging piece 202 shown in fig. 3, the side wall of the flow cell 100 is provided with a guide rod 201 pointing to the direction of the quantification groove 400 and the direction of the detection groove 500 in an extending manner as shown in fig. 4, the plugging piece 202 is connected with the guide rod 201 in a sliding manner and can move along the direction of the guide rod 201, the plugging piece 202 is provided with plugging heads 2023 which are equal to and adaptive to the number of the first flow channels 600, and after the plugging piece 202 moves along the guide rod 201, the plugging heads 2023 seal the first flow channels 600 to realize the isolation of the flow cell 100 and the quantification groove 400;
specifically, in this embodiment, the plugging member 202 is composed of two portions, i.e., a rod portion 2021 and a head portion 2022, which are fixedly connected, the head portion 2022 faces the quantitative groove 400 and the detection groove 500, the rod portion 2021 is U-shaped, a clamping block 2024 is disposed on the inner side of the rod portion 2021, the guide rod 201 is provided with a sliding groove 2011, the sliding connection between the plugging member 202 and the guide rod 201 is realized by the clamping of the clamping block 2024 and the sliding groove 2011, and the plugging head 2023 is disposed on the other side, back to the rod portion 2021, of the head portion 2022 of the plugging member 202.
When the sample adding groove is used in a centrifugal machine, the sample adding groove is arranged close to the inner side of the centrifugal machine and the rotating shaft center, and the detection groove 500 is arranged on the periphery;
under the cooperation of the structure, a monitoring and analyzing worker drops a sample to be detected through the sample adding groove, the sample to be detected enters the flow groove 100 after being dropped, the liquid level of the sample to be detected in the flow groove 100 rises to the height of the first flow channel 600, then the sample enters the quantitative groove 400 through the first flow channel 600, the liquid level in the quantitative groove 400 gradually rises, and after the liquid level reaches the specified liquid level, redundant samples to be detected are discharged into the waste liquid groove 300 through the third flow channel 800, so that the excessive adding of the sample to be detected does not need to be worried about in the using process, the step of adding the precise sample to be detected is omitted, the using efficiency is improved, the precise quantitative detection is ensured, and the problems of poor detection precision and the like caused by excessive or insufficient sample to be detected are avoided;
in addition, in the molecular diagnostic test card with the above structure, when the card is used in a centrifuge, the plugging member 202 rapidly moves along the guide rod 201 under the action of centrifugal force until the head 2022 of the plugging member 202 abuts against the wall of the pressure flow groove 100 and the plugging head 2023 plugs the first flow channel 600, so that the sample to be tested in the flow groove 100 under the action of centrifugal force can be prevented from entering the quantification groove 400 through the first flow channel 600 again, which results in the reduction of quantification accuracy, the sample to be tested in the quantification groove 400 can effectively enter the detection groove 500 under the action of centrifugal force through the second flow channel 700, and all the sample to be tested in the quantification groove 400 under the action of centrifugal force can enter the detection groove 500 to be effectively detected with the detection reagent;
through the cooperation of many cell bodies, multithread way and shutoff piece 202, can realize the quantitative sampling detection of higher accuracy, avoid because the liquid measure of the sample of waiting to survey disturbs the testing result.
In a further aspect of the present invention,
as shown in fig. 6, an elastic sealing body 900 is arranged in the second flow channel 700, the elastic sealing body 900 includes an elastic seat 901, a sealing ball 902 and a limiting net 903, and the limiting net 903 is an elastic fiber net;
the elastomer seat 901 is fixedly connected with the wall of the second flow channel 700 and is funnel-shaped, the tip of the elastomer seat with a mouth part faces the quantifying groove 400, the outer diameter of the sealing ball 902 is larger than the inner diameter of the mouth part, so that the sealing ball 902 cannot enter the quantifying groove 400 through the elastomer seat 901, the limiting net 903 is fixedly connected with the wall of the second flow channel 700 and presses the sealing ball 902 in the elastomer seat 901, so that the sealing ball 902 blocks the mouth part of the elastomer seat 901, and the integrally formed elastic sealing body 900 seals the second flow channel 700, so that a sample to be detected in the quantifying groove 400 cannot flow into the detection groove 500 through the second flow channel 700;
the elastic sealing body 900 can prevent the sample to be detected from flowing into the detection groove 500 due to too fast sample application, so that the existing test cards need to apply samples slowly, the detection efficiency is limited, once the test cards are placed with a certain inclination, a part of the sample to be detected is easy to overflow into the detection groove 500, and a certain use limitation exists, and after the elastic sealing body 900 is arranged, the sample to be detected can be effectively prevented from entering the detection groove 500 before centrifugal detection as shown in part 6-1 in fig. 6, and on the other hand, a reagent for detection in the detection groove 500 can be prevented from flowing back into the quantitative groove 400;
when the centrifugal force is applied to the centrifuge, as shown in fig. 6-2, the sealing ball 902 removes the blocking of the elastomer seat 901 under the centrifugal force, so that the sample to be detected in the quantitative groove 400 can effectively flow into the detection groove 500 through the second flow channel 700 for detection.
Through the cooperation of each above-mentioned structure, can improve detection efficiency, ensure to detect the precision to improve and use the portability, solved current molecular diagnosis test card use comparatively loaded down with trivial details, need the problem of accurate ration application of sample. Moreover, the whole process can be visually observed through the cover body 20, and the detection result can also be directly visually observed.
Claims (7)
1. A molecular diagnostic test card, comprising:
the body and the sealing cover are arranged on the cover body on the front surface of the body;
the body outside the sealing cover area of the cover body is provided with a sample adding groove, and the body in the sealing cover area of the cover body is provided with a flow groove, a waste liquid groove, a quantifying groove and a detection groove;
the flow groove is communicated with the sample adding groove and is communicated to the quantitative groove through a first flow passage, the quantitative groove is communicated with the detection groove through a second flow passage, and the flow groove is communicated to the waste liquid groove through a third flow passage;
the top of the first flow channel is lower than the bottom of the third flow channel, and the bottom of the second flow channel is higher than the bottom of the third flow channel;
be equipped with mobilizable shutoff piece in the chute, the shutoff piece carries out the shutoff to first flow passage under centrifugal action.
2. The molecular diagnostic test card of claim 1,
the flow groove is provided with a guide rod pointing to the direction of the quantitative groove and the direction of the detection groove;
the blocking piece is connected with the guide rod in a sliding mode and is provided with a blocking head matched with the first flow channel, and the blocking piece moves along the guide rod to close the first flow channel to isolate the quantitative groove and the flow groove.
3. The molecular diagnostic test card of claim 2,
the plugging piece comprises a rod portion and a head portion, the head portion faces the quantitative groove and the detection groove, the rod portion is U-shaped, the inner side of the rod portion is provided with a clamping block, the guide rod is provided with a sliding groove, the plugging piece and the guide rod are connected in a sliding mode through the clamping block and the sliding groove, and the plugging head is arranged on the other side, back to the rod portion, of the head portion of the plugging piece.
4. The molecular diagnostic test card according to claim 1, 2 or 3,
an elastic sealing body is arranged in the second flow channel.
5. The molecular diagnostic test card of claim 4,
the elastic sealing body comprises an elastic body seat, a sealing ball and a limiting net;
the projectile body seat is funnel-shaped and is fixedly connected with the second flow channel wall, the tip end of the projectile body seat is provided with a mouth and faces the quantitative groove, and the limiting net is fixedly connected with the second flow channel wall and tightly presses the sealing ball in the projectile body seat.
6. The molecular diagnostic test card of claim 5,
the limiting net is an elastic fiber net.
7. The molecular diagnostic test card according to claim 5 or 6,
the outer diameter of the sealing ball is larger than the inner diameter of the mouth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220233528.3U CN217377883U (en) | 2022-01-26 | 2022-01-26 | Molecular diagnosis test card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220233528.3U CN217377883U (en) | 2022-01-26 | 2022-01-26 | Molecular diagnosis test card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217377883U true CN217377883U (en) | 2022-09-06 |
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ID=83097098
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220233528.3U Active CN217377883U (en) | 2022-01-26 | 2022-01-26 | Molecular diagnosis test card |
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| Country | Link |
|---|---|
| CN (1) | CN217377883U (en) |
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2022
- 2022-01-26 CN CN202220233528.3U patent/CN217377883U/en active Active
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