CN217351403U - Suspension cell culture bottle - Google Patents
Suspension cell culture bottle Download PDFInfo
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- CN217351403U CN217351403U CN202123393854.5U CN202123393854U CN217351403U CN 217351403 U CN217351403 U CN 217351403U CN 202123393854 U CN202123393854 U CN 202123393854U CN 217351403 U CN217351403 U CN 217351403U
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- bottle
- cell culture
- control box
- suspension cell
- intake pipe
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Abstract
The utility model discloses a suspension cell culture bottle, the bottle comprises a bottle body, the top of bottle is provided with connection port, be provided with the bottle lid on the connection port, the top of bottle lid is provided with the motor, the bottom output of motor runs through the bottle lid and is provided with the pivot, the surface of pivot is provided with the puddler, one side of bottle is provided with temperature sensor, and temperature sensor's detection head extends to the bottle inner chamber, the front surface of bottle is provided with the control box, front surface one side of control box is provided with the display screen, the front surface opposite side of control box is provided with bee calling organ, the inner chamber of control box is provided with treater, time relay and transmission module, treater electrical property input connection temperature sensor and time relay. The utility model discloses possess the function that continuously stirs the liquid in the bottle, the warning staff that simultaneously can be regularly changes liquid.
Description
Technical Field
The utility model relates to the technical field of medical equipment, specifically a suspension cell culture bottle.
Background
Cell culture technology is also called cell cloning technology, and is formally termed in biology as cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. A large number of cells or their metabolites are obtained by cell culture. Since biological products are all derived from cells, it can be said that cell culture technology is the most core and basic technology in biotechnology.
Suspension culture refers to a tissue culture system for culturing single cells and small cell clusters in a liquid medium under constant agitation or shaking, a culture method of anchorage-independent cells, and some anchorage-dependent cells can be cultured by the method after adaptation and selection. The scale of suspension culture is relatively simple to increase, the depth is more than 5mm, the culture medium needs to be stirred and is more than 10cm, carbon dioxide and air need to be introduced deeply to ensure sufficient gas exchange, and the cells are always in a culture method of dispersing and suspending in the culture solution by oscillating or rotating the device.
The existing suspension cell culture bottle has some defects in the using process, for example, the liquid in the culture bottle is not convenient to stir continuously, and meanwhile, the function of reminding the user of changing the liquid at regular time is not available.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a suspension cell culture bottle possesses the function that continuously stirs the liquid in the bottle, and the warning staff that can be regularly simultaneously changes liquid.
The utility model provides a following technical scheme: the utility model provides a suspension cell culture bottle, includes bottle, motor, puddler and control box, the top of bottle is provided with connection port, is provided with the bottle lid on the connection port, the motor is installed on the top of bottle lid, and the bottom output of motor runs through the bottle lid and is provided with the pivot, the puddler is installed in the pivot, be provided with a minute mouth pipe on the bottle lid, minute mouth pipe and bottle inner chamber intercommunication, minute mouth pipe inner chamber joint have hydrophobic ventilated membrane, one side of bottle is provided with temperature sensor, the control box is installed on the bottle, be provided with display screen and bee calling organ on the control box, independent power supply respectively with display screen and bee calling organ electric connection, the inner chamber of control box is provided with treater, time relay and transmission module, treater electrical property input connection temperature sensor and time relay, treater electrical output connection display screen, Buzzer and transmission module, transmission module electrical output connects handheld terminal.
Furthermore, the surfaces of the rotating shaft and the stirring rod are provided with anti-corrosion coatings.
Furthermore, the connection port and the bottle cap are provided with matched threads, and the connection port is in threaded connection with the bottle cap.
Furthermore, a battery box is arranged on the bottle cap, a battery is arranged in the battery box, and the battery is electrically connected with the motor.
Furthermore, a plurality of elastic abutting pieces are arranged on the inner wall of the opening pipe, the elastic abutting pieces are arranged in a surrounding mode, and a circular through hole is formed in the middle of the elastic abutting pieces.
Further, the handheld terminal is one of a smart phone, a notebook computer and a tablet computer.
Furthermore, the inner cavity of the control box is provided with an independent power supply, and the independent power supply is preferably a button cell.
Furthermore, a liquid outlet is formed in the bottom of one side of the bottle body, and a valve is arranged on the liquid outlet.
Further, the bottom of bottle is provided with the intake pipe, and the intake pipe includes air intake pipe and carbon dioxide intake pipe, and air intake pipe and carbon dioxide intake pipe pass through a branch and connect.
Compared with the prior art, the beneficial effects of the utility model are as follows:
1. the utility model discloses a be provided with the motor at the top of bottle lid, be provided with the pivot in the bottom of bottle lid, be provided with the puddler on the surface of pivot, realized also continuous stirring of liquid to the bottle lid, guarantee the quality of cultivateing.
2. The utility model discloses a be provided with temperature sensor in one side of bottle, realized temperature monitoring, send signal for the treater through time relay, treater control bee calling organ sends out the police dispatch newspaper, reminds the staff to trade liquid, has guaranteed the quality of cultivation, more intelligent.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic side view of the present invention;
fig. 3 is a schematic diagram of the system circuit frame structure of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
As shown in fig. 1 and 2, a suspension cell culture bottle comprises a bottle body 1, a motor 4 and a stirring rod 6, wherein the bottle body 1 is used for bearing a culture medium and cells, a connection port 2 is arranged at the top of the bottle body 1, a bottle cap 3 is arranged on the connection port 2, matching threads are arranged on the connection port 2 and the bottle cap 3, the connection port 2 is in threaded connection with the bottle cap 3, the motor 4 is arranged at the top end of the bottle cap 3, a rotating shaft 5 is arranged at the bottom output end of the motor 4 and penetrates through the bottle cap 3, the stirring rod 6 is arranged on the rotating shaft 5, the stirring rod 6 is used for stirring liquid, and anti-corrosion coatings are arranged on the surfaces of the rotating shaft 5 and the stirring rod 6 so as to prevent the rotating shaft 5 and the stirring rod 6 from being corroded by being soaked for a long time, so that the service life is prolonged, and the detection accuracy can be ensured; the bottle cap 3 is provided with a battery box 16, a battery is arranged in the battery box 16, and the battery is electrically connected with the motor 4 and used for providing a power supply for the motor 4 to work; the bottle cap 3 is provided with a tapping pipe 17, the tapping pipe 17 is communicated with the inner cavity of the bottle body 1, the tapping pipe 17 is used for throwing cells and exchanging air, a hydrophobic breathable film is clamped in the inner cavity of the tapping pipe 17 and can isolate water from entering, the inner wall of the tapping pipe 17 is provided with a plurality of elastic abutting pieces 171, the elastic abutting pieces 171 are arranged in a surrounding mode, a circular through hole is formed in the middle of the elastic abutting pieces 171, and the elastic abutting pieces 171 are arranged to be used for inserting test tubes with different diameters.
One side of bottle 1 is provided with temperature sensor 11, and temperature sensor 11's detection head extends to 1 inner chamber of bottle, and temperature sensor 11 is used for detecting the interior liquid temperature of bottle, install a control box 8 on the bottle 1, the inner chamber of control box 8 is provided with independent power supply, and independent power supply is preferred to be button cell, be provided with display screen 9 and bee calling organ 10 on the control box 8, independent power supply respectively with display screen 9 and bee calling organ 10 electric connection for provide the required power of its work, wherein, display screen 9 is used for showing temperature, the information such as volume of liquid in the bottle 1, bee calling organ 10 is used for the alarming action, the inner chamber of control box 8 is provided with treater 12, time relay 13 and transmission module 14.
As shown in fig. 3, the processor 12 is electrically connected to the temperature sensor 11 and the time relay 13, the processor 12 is electrically connected to the display 9, the buzzer 10 and the transmission module 14, the transmission module 14 is electrically connected to the handheld terminal 15, the handheld terminal 15 is one of a smart phone, a notebook computer or a tablet computer, the liquid discharge port 7 is arranged at the bottom of one side of the bottle body 1, a valve is arranged on the liquid discharge port 7, and liquid in the bottle body 1 is discharged through the liquid discharge port 7.
The bottom of bottle 1 is provided with the intake pipe, and the intake pipe includes air intake pipe 18 and carbon dioxide intake pipe 19, and air intake pipe 18 and carbon dioxide intake pipe 19 are connected through a branch joint, and air intake pipe 18 is used for carrying the air, and carbon dioxide intake pipe 19 is used for carrying carbon dioxide gas.
The working principle of the utility model is as follows: at first put into bottle 1 with the culture solution in, starter motor 4, pivot 5 drive puddler 6 is rotatory, realized the continuous stirring to liquid in the bottle, guarantee cultivated quality, let in carbon dioxide gas or air simultaneously, temperature sensor 11 realizes temperature real time monitoring during the stirring, along with the lapse of time, 13 send signal for treater 12 through time relay, treater 12 control bee calling organ 10 sends out the police dispatch newspaper, remind the staff to change the liquid, the quality of cultivateing has been guaranteed, more intellectuality.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (8)
1. The utility model provides a suspension cell culture bottle, includes bottle, motor, puddler and control box, its characterized in that: the top of the bottle body is provided with a connecting port, the connecting port is provided with a bottle cap, the motor is arranged at the top end of the bottle cap, the bottom output end of the motor penetrates through the bottle cap to be provided with a rotating shaft, the stirring rod is arranged on the rotating shaft, the bottle cap is provided with a tapping pipe, the tapping pipe is communicated with the inner cavity of the bottle body, the inner wall of the tapping pipe is provided with a plurality of elastic abutting pieces, the elastic abutting pieces are arranged in a surrounding way, a round through hole is formed in the middle of the elastic abutting pieces, the inner cavity of the tapping pipe is clamped with a hydrophobic breathable film, one side of the bottle body is provided with a temperature sensor, the control box is arranged on the bottle body, the control box is provided with a display screen and a buzzer, an independent power supply is respectively and electrically connected with the display screen and the buzzer, the inner cavity of the control box is provided with a processor, a time relay and a transmission module, and the processor is electrically connected with the temperature sensor and the time relay in an input mode, the processor is electrically connected with the display screen, the buzzer and the transmission module in an output mode, and the transmission module is electrically connected with the handheld terminal in an output mode.
2. The suspension cell culture flask of claim 1, wherein: and the surfaces of the rotating shaft and the stirring rod are provided with anti-corrosion coatings.
3. The suspension cell culture flask of claim 1, wherein: and the connecting port and the bottle cap are provided with matched threads, and the connecting port is in threaded connection with the bottle cap.
4. The suspension cell culture flask of claim 1, wherein: the bottle cap is provided with a battery box, a battery is arranged in the battery box, and the battery is electrically connected with the motor.
5. The suspension cell culture flask of claim 1, wherein: the handheld terminal is one of a smart phone, a notebook computer or a tablet computer.
6. The suspension cell culture flask of claim 1, wherein: an independent power supply is arranged in the inner cavity of the control box and is a button cell.
7. The suspension cell culture flask of claim 1, wherein: a liquid outlet is formed in the bottom of one side of the bottle body, and a valve is arranged on the liquid outlet.
8. The suspension cell culture flask of claim 1, wherein: the bottom of bottle is provided with the intake pipe, and the intake pipe includes air intake pipe and carbon dioxide intake pipe, and air intake pipe and carbon dioxide intake pipe pass through a branch and connect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123393854.5U CN217351403U (en) | 2021-12-30 | 2021-12-30 | Suspension cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123393854.5U CN217351403U (en) | 2021-12-30 | 2021-12-30 | Suspension cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217351403U true CN217351403U (en) | 2022-09-02 |
Family
ID=83043182
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123393854.5U Active CN217351403U (en) | 2021-12-30 | 2021-12-30 | Suspension cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217351403U (en) |
-
2021
- 2021-12-30 CN CN202123393854.5U patent/CN217351403U/en active Active
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