CN217212248U

CN217212248U - Sample holder for solid powder laser-induced fluorescence test and fluorescence spectrophotometer

Info

Publication number: CN217212248U
Application number: CN202122297915.1U
Authority: CN
Inventors: 雷涛; 郑则原; 张汉; 张瑞; 杨仁杰
Original assignee: Tianjin Agricultural University
Current assignee: Tianjin Agricultural University
Priority date: 2021-09-22
Filing date: 2021-09-22
Publication date: 2022-08-16
Anticipated expiration: 2031-09-22

Abstract

The utility model discloses a sample frame for solid powder laser induced fluorescence test, including base, the fixed module of sample and the module of exerting pressure, the fixed module of sample is all adorned on the base with the module of exerting pressure admittedly, and the fixed module of sample includes: fixed panel of sample, laser penetrate into panel and laser and jet out the panel, and fixed panel of sample, laser penetrate into panel and laser and jet out the panel and enclose into a top surface and the equal open triangular prism structure in bottom surface, and the module of exerting pressure includes: the fixing piece is fixedly installed with the base, one end of the supporting rod is fixedly installed with the side face of the fixing piece, the pressure spring is sleeved on the supporting rod, one end of the pressure spring is connected with one end, close to the fixing piece, of the supporting rod, and the other end of the pressure spring is connected with the top plate. Reequip the installation on current fluorescence spectrophotometry with xenon lamp as the basis the utility model discloses a sample frame, supplementary optic fibre formula laser instrument carries out laser induction fluorescence test to solid powder, realizes the detection of trace material in the complex system, and is with low costs, the sexual valence relative altitude.

Description

Sample holder for solid powder laser-induced fluorescence test and fluorescence spectrophotometer

Technical Field

The utility model belongs to the technical field of fluorescence test, a sample frame and fluorescence spectrophotometer for solid powder laser induced fluorescence test is related to particularly.

Background

The fluorescence spectrum technology has the characteristics of good selectivity, high sensitivity, good reproducibility, no need of pretreatment and the like, and is widely applied to detection in various fields of biology, medicine, environment and the like. The conventional fluorescence spectrophotometer in a laboratory generally adopts xenon lamps, light emitted by a xenon lamp light source is distributed in a light range of 200-800 nm, and when a fluorescence emission spectrum is mapped, the light in the light range is converted into monochromatic light through an excitation light monochromator, namely, the optimal excitation wavelength is fixed, for example, a pulse xenon lamp is adopted by LS-55 of American PE, and a continuous xenon lamp is adopted by RF-5301 of Shimadzu, Japan. For the detection of trace substances in a complex system, on one hand, the xenon lamp cannot be effectively excited due to low energy; on the other hand, the signal-to-noise ratio is poor, and the quantitative analysis of the signal-to-noise ratio cannot be carried out. For example, a laboratory fluorescence spectrophotometer is used to directly detect polycyclic aromatic hydrocarbons in soil, and due to the complexity of the soil and the trace amount of PAHs in the soil, when the concentration of PAHs pollutants in the soil is lower than 10 ^-3 In g/g, the common pulse xenon lamp cannot be effectively excited due to low energy, so that the requirement of practical application cannot be met.

Because of the characteristics of high brightness, good monochromaticity and no stray light of the laser light source, the laser-induced fluorescence spectroscopy technology is widely applied to various fields as a rapid and high-sensitivity detection means capable of realizing real-time and in-situ detection. Compared with the traditional fluorescence detection technology, the concentration detection limit of the laser-induced fluorescence detection technology can reachTo 10 ^-13 mol/L, has higher sensitivity, and is particularly suitable for detecting trace or even trace substances in a complex system. Therefore, in order to meet the detection requirement, a laser-induced fluorescence detection system has been developed, but the equipment cost is high.

Disclosure of Invention

In order to solve the technical scheme not enough, the utility model aims to provide a sample frame for solid powder laser induced fluorescence test, this sample frame can be as independent light source at an external fiber laser on the spectrofluorometer that adopts the xenon lamp to build a solid powder laser induced fluorescence test system who satisfies the detection requirement, realize simple and easy quick installation, have simple structure, with low costs, easy abluent characteristics.

The purpose of the utility model is realized by the following technical scheme.

A sample holder for solid powder laser-induced fluorescence test comprises a base, a sample fixing module and a pressure applying module, wherein the sample fixing module and the pressure applying module are fixedly arranged on the base, the base is horizontally and fixedly arranged in a sample chamber of a fluorescence spectrophotometer, the sample holder comprises a base, a sample fixing module and a pressure applying module,

the sample holding module includes: the laser injection panel and the laser injection panel are fixedly arranged on two opposite edges of the sample fixing panel, so that the sample fixing panel, the laser injection panel and the laser injection panel enclose a triangular prism structure with an open top surface and an open bottom surface;

a first through hole is formed on the laser incidence panel and used for fixing a fiber probe of a fiber laser, a second through hole for fixing a solid powder sample cell is formed on the sample fixing panel, so that laser emitted by the fiber probe is emitted on a sample of the solid powder sample cell, and a window is formed on the laser emission panel and used for emitting laser reflected by the sample;

the pressure application module includes: mounting, branch, pressure spring and top flat, the bottom of mounting with the base is adorned admittedly, the branch level set up and the one end of this branch with the side of mounting is adorned admittedly, the pressure spring cover is in on the branch, the one end of pressure spring with branch is close to the one end of mounting and is connected, and the other end is connected the top flat, the top flat will solid powder sample pool impresses the second through-hole.

In the above technical scheme, solid powder sample cell includes casing, printing opacity piece and collet, the casing is a top and the equal open cylindrical in bottom, the top edge of casing is formed with a first spacing collar to the axle center direction, printing opacity piece quilt first spacing collar spacing in the casing and with the top surface of casing is parallel, the one end of collet is located in the casing and with the inner wall threaded connection of casing, the collet is pressed the sample on the printing opacity piece.

In the above technical solution, the top surface of the solid powder sample cell is located in the triangular prism structure.

In the above technical solution, the housing tapers from the bottom surface to the top surface.

In the above technical solution, a second stop collar is formed outside the circumference of the housing, the diameter of the second stop collar is larger than the diameter of the second through hole, and the top plate presses the second stop collar on the sample fixing panel.

In the above technical solution, an included angle between the laser incident panel and the laser emergent panel is 120 °.

In the above technical solution, a third through hole is formed in the base for passing through the optical fiber.

A fluorescence spectrophotometer is characterized in that a sample chamber of the fluorescence spectrophotometer is internally and fixedly provided with a sample rack.

In the above technical solution, the fluorescence spectrophotometer includes: and the optical fiber probe of the optical fiber type laser penetrates into a sample chamber of the fluorescence spectrophotometer and is fixed on the first through hole.

Reequip the installation on current fluorescence spectrophotometry based on the xenon lamp the utility model discloses a sample holder, the utility model discloses a sample holder assists optic fibre formula laser instrument to carry out laser induction fluorescence test to solid powder, realizes the detection of trace material in the complex system, and is with low costs, the sexual valence relative altitude.

Drawings

Fig. 1 is a schematic structural diagram of a sample holder according to the present invention;

FIG. 2 is a schematic diagram of a solid powder sample cell;

FIG. 3 is a schematic view of a sample mounting plate;

FIG. 4 is a schematic view of the construction of a fluorescence spectrophotometer;

fig. 5 is a schematic structural view of a triangular prism structure.

1: topsheet, 2: third via hole, 3: laser incident panel, 4: first through hole, 5: window, 6: laser emission panel, 7: second through hole, 8: sample fixing panel, 9: base, 10: fixing member, 11: pressure spring, 12: strut, 13: solid powder sample cell, 13-1: first stop collar, 13-2: shell, 13-3: light-transmitting sheet, 13-4: shoe, 14: fiber laser, 15: a sample chamber.

Detailed Description

The sample holder for solid powder laser induced fluorescence test of the present invention is described in detail below with reference to the accompanying drawings.

The spectrofluorometer of the following examples is model LS-55 spectrofluorometer.

Example 1

As shown in fig. 1, the fluorescence spectrophotometer comprises a base 9, a sample fixing module (not shown) and a pressure applying module (not shown), wherein the sample fixing module and the pressure applying module are both fixedly arranged on the base 9, an original sample holder in a sample chamber 15 of the fluorescence spectrophotometer is disassembled, the base 9 can be horizontally and fixedly arranged in the sample chamber 15 of the fluorescence spectrophotometer through bolts, wherein,

the sample fixing module includes: the laser injection panel 3 and the laser injection panel 6 are fixedly arranged on two opposite edges of the sample fixing panel 8, so that the sample fixing panel 8, the laser injection panel 3 and the laser injection panel 6 enclose a cavity with a triangular prism structure with an open top surface and an open bottom surface, as shown in fig. 5;

a first through hole 4 is formed on the laser injection panel 3 and used for fixing a fiber probe of the fiber laser 14, the fiber probe is installed in the first through hole 4 and can be in threaded connection with the first through hole 4, and the fiber probe is connected with the output end of the fiber laser through an optical fiber. A second through hole 7 for fixing a solid powder sample cell 13 is formed in the sample fixing panel 8, as shown in fig. 3, so that the laser emitted from the fiber optic probe is incident on the sample of the solid powder sample cell 13, and a window 5 is formed in the laser emitting panel 6, and the size of the window 5 is as follows: the length is 20mm, the width is 10mm, and the laser light which passes through the sample after being reflected is received by the photoelectric detector;

the pressure application module comprises: the solid powder sample pool fixing device comprises a fixing piece 10, a supporting rod 12, a pressure spring 11 and a top plate 1, wherein the bottom end of the fixing piece 10 is fixedly installed on a base 9, the supporting rod 12 is horizontally arranged, one end of the supporting rod 12 is fixedly installed on the side face of the fixing piece 10, the pressure spring 11 is sleeved on the supporting rod 12, one end of the pressure spring 11 is connected with one end, close to the fixing piece 10, of the supporting rod 12, the other end of the pressure spring is connected with the top plate 1, the pressure spring drives the top plate 1 to press a solid powder sample pool 13 into a second through hole 7, and the second through hole 7 is matched with a fixing device of the solid powder sample pool 13 to fix the solid powder sample pool 13.

Example 2

On the basis of the embodiment 1, as shown in fig. 2, the solid powder sample cell 13 includes a housing 13-2, a light-transmitting sheet 13-3 and a bottom support 13-4, the housing 13-2 is a cylindrical cavity with both open top and bottom ends, a first limiting ring 13-1 is formed on the top end edge of the housing 13-2 toward the axial direction, the light-transmitting sheet 13-3 is limited in the housing 13-2 by the first limiting ring 13-1 and is parallel to the top surface of the housing 13-2, one end of the bottom support 13-4 is located in the housing 13-2 and is in threaded connection with the inner wall of the housing 13-2, and when the bottom support 13-4 is in threaded connection with the housing 13-2, the bottom support 13-4 presses the sample on the light-transmitting sheet 13-3 to fix the sample.

When the solid powder sample cell 13 is installed in the second through hole 7, the top surface of the solid powder sample cell 13 is located in the triangular prism structure, i.e., one side of the light-transmitting sheet 13-3 is located in the triangular prism structure.

Preferably, the included angle between the laser incident panel 3 and the laser emergent panel 6 is 120 °, and experiments prove that the laser-induced fluorescence intensity can be effectively improved when the included angle is less than 45 °.

A third through-hole 2 is formed in the base 9 for passing the optical fiber therethrough.

Example 3

Based on example 2, the housing 13-2 is tapered from the bottom surface to the top surface, and when the top plate 1 presses the solid powder sample cell 13 into the second through hole 7, the tapered structure can fix the solid powder sample cell 13.

Example 4

In example 2, a second stop collar (not shown) is formed outside the circumference of the housing 13-2, the diameter of the second stop collar is larger than the diameter of the second through hole 7, and the top plate 1 presses the second stop collar against the sample fixing panel 8, so as to fix the solid powder sample cell 13.

Example 5

As shown in fig. 4, a fluorescence spectrophotometer includes: the sample holder is characterized by comprising an optical fiber type laser 14 and a sample holder which is fixedly arranged in a sample chamber 15 and is provided with any one of embodiments 1-4, wherein an optical fiber probe of the optical fiber type laser 14 penetrates into the sample chamber 15 of the fluorescence spectrophotometer and is in threaded connection with the first through hole 4.

The invention has been described above by way of example, and it should be noted that any simple variants, modifications or other equivalent substitutions by a person skilled in the art without spending creative effort may fall within the scope of protection of the present invention without departing from the core of the present invention.

Claims

1. A sample holder for solid powder laser-induced fluorescence test is characterized by comprising a base (9), a sample fixing module and a pressure applying module, wherein the sample fixing module and the pressure applying module are fixedly arranged on the base (9), the base (9) is horizontally and fixedly arranged in a sample chamber (15) of a fluorescence spectrophotometer, the sample holder is provided with a sample fixing module and a pressure applying module, the sample fixing module and the pressure applying module are arranged on the sample chamber,

the sample holding module includes: the laser injection panel (3) and the laser injection panel (6) are fixedly arranged on two opposite edges of the sample fixing panel (8), so that the sample fixing panel (8), the laser injection panel (3) and the laser injection panel (6) enclose a triangular prism structure with an open top surface and an open bottom surface;

a first through hole (4) is formed on the laser incidence panel (3) and is used for fixing a fiber probe of a fiber laser (14), a second through hole (7) is formed on the sample fixing panel (8) and is used for fixing a solid powder sample cell (13) so that the laser emitted by the fiber probe is emitted on a sample of the solid powder sample cell (13), and a window (5) is formed on the laser emission panel (6) and is used for emitting the laser reflected by the sample;

the pressure application module includes: mounting (10), branch (12), pressure spring (11) and top plate (1), the bottom of mounting (10) with base (9) are adorned admittedly, branch (12) level set up and the one end of this branch (12) with the side of mounting (10) is adorned admittedly, pressure spring (11) cover is in on branch (12), the one end of pressure spring (11) with branch (12) are close to the one end of mounting (10) and are connected, and the other end is connected top plate (1), top plate (1) will solid powder sample cell (13) are impressed second through-hole (7).

2. The sample holder according to claim 1, wherein the solid powder sample cell (13) comprises a housing (13-2), a light transmissive sheet (13-3) and a base support (13-4), the shell (13-2) is in a cylindrical shape with both the top end and the bottom end open, a first limiting ring (13-1) is formed at the edge of the top end of the shell (13-2) towards the axis direction, the light-transmitting sheet (13-3) is limited in the shell (13-2) by the first limiting ring (13-1) and is parallel to the top surface of the shell (13-2), one end of the bottom support (13-4) is positioned in the shell (13-2) and is in threaded connection with the inner wall of the shell (13-2), and the bottom support (13-4) presses the sample on the light-transmitting piece (13-3).

3. A sample holder as claimed in claim 2, characterized in that the top surface of the solid powder sample cell (13) is located within the triangular prism structure.

4. A sample holder as claimed in claim 3, wherein the housing (13-2) tapers from a bottom surface to a top surface.

5. A sample holder as claimed in claim 3, characterized in that a second stop collar is formed outside the circumference of the housing (13-2), the diameter of the second stop collar being larger than the diameter of the second through hole (7), the top sheet (1) pressing the second stop collar against the sample-holding panel (8).

6. A sample holder as claimed in claim 4 or 5, characterized in that the angle between the laser entry panel (3) and the laser exit panel (6) is 120 °.

7. A sample holder as claimed in claim 6, characterized in that a third through-hole (2) is formed in the base (9) for the passage of an optical fibre.

8. A spectrofluorometer characterized in that the sample holder according to any of claims 1-7 is mounted in the sample chamber (15) of the spectrofluorometer.

9. The spectrofluorometer of claim 8, comprising: the optical fiber type laser (14), the optical fiber probe of the optical fiber type laser (14) penetrates into the sample room (15) of the fluorescence spectrophotometer and is fixed on the first through hole (4).