CN108152252A - A kind of integration type femtosecond time resolution fluorescence lifetime measurement spectrometer - Google Patents
A kind of integration type femtosecond time resolution fluorescence lifetime measurement spectrometer Download PDFInfo
- Publication number
- CN108152252A CN108152252A CN201611101990.3A CN201611101990A CN108152252A CN 108152252 A CN108152252 A CN 108152252A CN 201611101990 A CN201611101990 A CN 201611101990A CN 108152252 A CN108152252 A CN 108152252A
- Authority
- CN
- China
- Prior art keywords
- femtosecond
- fluorescence
- sample
- laser
- fluorescence lifetime
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000010354 integration Effects 0.000 title claims abstract description 29
- 238000005259 measurement Methods 0.000 title claims abstract description 28
- 238000001514 detection method Methods 0.000 claims abstract description 28
- 230000003595 spectral effect Effects 0.000 claims abstract description 13
- 238000005086 pumping Methods 0.000 claims abstract description 5
- 230000003287 optical effect Effects 0.000 claims description 15
- 238000001228 spectrum Methods 0.000 claims description 13
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 6
- 239000013307 optical fiber Substances 0.000 claims description 5
- 230000011664 signaling Effects 0.000 claims description 5
- PLXMOAALOJOTIY-FPTXNFDTSA-N Aesculin Natural products OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1Oc2cc3C=CC(=O)Oc3cc2O PLXMOAALOJOTIY-FPTXNFDTSA-N 0.000 claims 1
- 230000005855 radiation Effects 0.000 abstract description 6
- 230000005284 excitation Effects 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 29
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 5
- 230000002123 temporal effect Effects 0.000 description 5
- WISWLZYHZLVSMO-UHFFFAOYSA-N 6-phenyl-2-(4-phenylphenyl)-1,3-benzoxazole Chemical compound C1=CC=CC=C1C1=CC=C(C=2OC3=CC(=CC=C3N=2)C=2C=CC=CC=2)C=C1 WISWLZYHZLVSMO-UHFFFAOYSA-N 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 238000004611 spectroscopical analysis Methods 0.000 description 4
- 239000000975 dye Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 229910000737 Duralumin Inorganic materials 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000010351 charge transfer process Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000002189 fluorescence spectrum Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005316 response function Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention relates to time resolution fluorescence spectral measuring instruments, particularly relate to a kind of integration type femtosecond time resolution fluorescence lifetime measurement spectrometer, including detection and pumping laser light path system, fluorescent acquisition system and spectral detection system.This spectrometer excites sample using a branch of femtosecond laser, another beam femtosecond laser causes the sample stimulated radiation of excitation state, all fluorescents being collected into are integrated in spectral detection system, so as to fulfill the measurement of fluorescence lifetime, invention increases the detection sensitivities of fluorescence lifetime measurement.
Description
Technical field
The present invention relates to time resolved spectroscopy measuring instruments, particularly relate to a kind of integration type femtosecond time resolution fluorescence longevity
Measure spectrum instrument is ordered, this spectrometer excites sample using a branch of femtosecond laser, then to excite using another beam femtosecond laser
The sample stimulated radiation of state so that the fluorescence volume of sample free radiation is reduced, and spectral detection system is by all samples being collected into
Fluorescence is integrated, and compares the Fluorescence integral intensity for having stimulated radiation and during without stimulated radiation, obtains fluorescence lifetime letter
Number point coordinates time-delay technique, realizes the fluorescence lifetime for measuring femtosecond time resolution, invention increases fluorescence lifetime measurements
Detection sensitivity.
Background technology
Time resolution pumping-detection technology [volumes such as ultrafast laser spectral principle and technical foundation, Weng Yuxiang, Chen Hailong,
, Chemical Industry Press in 2013], also known as pump probe double-pulsed technology [time resolved spectroscopy basis, Guo's plinth, 2012
Year, Higher Education Publishing House], it has a wide range of applications in the numerous areas of modern science, as photophysical process, photochemistry are anti-
Should, Biochemical processes, light-catalyzed reaction, energy and charge transfer process, Characterization of Nano-materials etc..Fluorescence lifetime measurement is in life
The analysis of object fluorescence probe, Solar use, fluorescent molecular optical physics spectrochemical property, substance excitation state dynamics research etc.
There are many applications；Femtosecond time resolution spectral instrument of the present invention equally applies pumping-detection technology.Han Ke profits et al.
[Chinese Patent Application No.：201310392018.6] establish femtosecond time resolution transient absorption and the two-in-one spectrum of fluorescence deficit
Two kinds of pumping-detection technologies are attached in a spectrometer, extend the application of femtosecond time resolution spectral instrument by instrument.
The current more common main having time correlated single photon counter of fluorescence lifetime measurement instrument, streak camera, femtosecond
Time-resolved fluorescence upconversion emission instrument, femtosecond time resolution Fluorescence depletion spectroscopy instrument etc..Single Photon Counting device
Temporal resolution highest is also at tens picoseconds；The temporal resolution of streak camera reaches as high as picosecond, but price is very
It is expensive；The temporal resolution of femtosecond time resolution fluorescence upconversion emission instrument is limited by upper conversion crystal, and general ratio is used
Femto-second laser pulse width it is twice big；The temporal resolution of femtosecond time resolution Fluorescence depletion spectroscopy instrument is very high, still
It often only measures a wavelength of fluorescence when measuring fluorescence, in terms of reducing its fluorescence lifetime measurement in hypofluorescence substance
Using.
Invention content
It is an object of the invention to obtain a kind of integration type femtosecond time resolution fluorescence lifetime measurement spectrometer, should have very
Good temporal resolution will also have good fluorescence intensity detection limit.
To achieve these goals, the technical solution adopted by the present invention is：
Integration type femtosecond time resolution fluorescence lifetime measurement spectrometer, including detection and pumping laser light path system, sample
Fluorescent collecting system and spectral detection system；
All components are all placed on optical flat；
Femtosecond laser detects light beam, and the sample in sample cell is in spatially cross-coincidence with femtosecond laser pump beam；
Femtosecond laser detection light beam spatially intersects, and have certain intervals in time with femtosecond laser pump beam,
This time interval is adjusted by time delay device；
Femtosecond laser detection light beam is after being adjusted by time delay device, by delayer back mirror and sample cell
Front mirror realizes space crossed overlap on sample cell with femtosecond laser pump beam；
Fluorescent acquisition system is after the fluorescence that sample is radiated is collected by phosphor collection mirror, to be reflected by fluorescent reflection mirror
Onto fluorescent foci mirror, focused on fiber entrance by fluorescent foci mirror, sub-ray spectrometer is then imported by optical fiber；
Spectral measurement system be fluorescence after sub-ray spectrometer is divided, multi-color spectrum is formed on the detector, on detector
Multi-color spectrum integrated to obtain fluorescence integrated signal by computer；
After optical chopper operation, no femtosecond laser detection light beam will be obtained on computers and has femtosecond laser to detect light
Two fluorescence integrated signals of beam, the difference of the two fluorescence integrated signals is exactly fluorescence lifetime signaling point；
Fluorescence lifetime signaling point is depicted as delay time curve on computers, just obtains the integration type femtosecond of sample
Time-resolved fluorescence life curve.
Optical flat material be duralumin or stainless steel, dimensions：Long is about 120cm, and wide about 60cm, thickness are about
1cm；
The phosphor collection mirror and fluorescent foci mirror can be but not limited to lens, paraboloidal mirror, spherical mirror；
The sample cell is the cuvette that light path is 1mm or 2mm or 10mm；
The optical chopper, triggering frequency signal are set in 20Hz from femto-second laser, chopping frequency
To between 1000Hz；
The detector can be but not limited to photodiode array, charge coupled cell.
Description of the drawings
Fig. 1 is the integration type femtosecond time resolution fluorescence lifetime measurement spectrometer architecture schematic diagram of the present invention.
Fig. 2 is the integration type femtosecond time resolution fluorescence of a concentration of 1mmol/L PBBO dyestuff ethanol solutions in embodiment 1
Lifetime measurement curve.
Fig. 3 is the integration type femtosecond time resolution of a concentration of 0.1mmol/L OX750 dyestuff ethanol solutions in embodiment 2
Fluorescence lifetime measurement curve.(a) the spectrum evolution curve that OX750 solution fluorescences spectrum changes over time；(b) it is glimmering at 660nm
Curve (blue filled box) that luminous intensity changes with delay time and by total fluorescence after fluorescence intensity integration at all wavelengths
The curve (red short side frame) that intensity changes with delay time.
Specific embodiment
Attached drawing is please referred to, Fig. 1 is the structural diagram of the present invention.The integration type femtosecond time resolution fluorescence lifetime of the present invention
Optical flat length described in measure spectrum instrument is about 120cm, and wide about 60cm, there are two light inlet, the first light inlet 2 is femtosecond
Laser acquisition beam inlet, for stimulated radiation sample；Second light inlet 3 is femtosecond laser pump beam entrance, for exciting
Sample.
All components are all placed on optical flat 1, and femtosecond laser detection light beam 2 is with femtosecond laser pump beam 3 in sample
In spatially cross-coincidence where sample in product pond 4, femtosecond laser detection light beam 2 is after being adjusted by time delay device 5,
By delayer back mirror 6 and sample cell front mirror 7, pumped where the sample in sample cell 4 with femtosecond laser
Light beam 3 is in spatially cross-coincidence；
Femtosecond laser is submitted where detecting the sample of light beam 2 and femtosecond laser pump beam 3 in sample cell 4 in space
Fork overlaps and has specified time interval, this time interval is adjusted by time delay device 5；
Fluorescent acquisition system is after the fluorescence that sample is radiated is collected by phosphor collection mirror 8, by fluorescent reflection mirror 9 instead
It is mapped on fluorescent foci mirror 10, is focused on 11 entrance of optical fiber by fluorescent foci mirror 10, light splitting is then imported by optical fiber
Spectrometer 12；
Spectral measurement system is fluorescence after the light splitting of sub-ray spectrometer 12, and multi-color spectrum is formed on detector 13, is detected
Multi-color spectrum on device 13 carries out Integral Processing by computer 14 and obtains fluorescence integrated signal；
It, will be obtaining no femtosecond laser detection light beam on computers and have femtosecond laser spy after optical chopper 15 is run
Two fluorescence integrated signals of light beam are surveyed, the difference of the two fluorescence integrated signals is exactly fluorescence lifetime signaling point；
Fluorescence lifetime signaling point is depicted as delay time to curve on computer 14 and is fitted, just obtains sample
Integration type femtosecond time resolution fluorescence lifetime.
Phosphor collection mirror 8 and fluorescent foci mirror 10 can be but not limited to lens, paraboloidal mirror or spherical mirror；
Sample cell 4 is the cuvette that light path is 1mm or 2mm or 10mm；
Optical chopper 15, triggering frequency signal are set in 20Hz extremely from femto-second laser, chopping frequency
Between 1000Hz；
Detector 13 can be but not limited to photodiode array or charge coupled cell.
Embodiment 1
Integration type femtosecond is being measured for integration type femtosecond time resolution fluorescence lifetime measurement spectrometer of the present invention
The time resolution in performance during the time-resolved fluorescence service life is investigated, molten using the PBBO dyestuff ethyl alcohol of 1m mol/L
Liquid is test specimen, measures integration type femtosecond time resolution fluorescence lifetime signal.The absorption spectrum peak value of PBBO is attached in 320nm
Closely, only when 490nm pump beams and 800nm detection light beam two light pulses on room and time all in sample cell
When being overlapped at sample, the fluorescence of PBBO can be just inspired, it is hereby achieved that integration type femtosecond time resolution fluorescence lifetime measurement
The time resolution receptance function of spectrometer.The Instrument response function obtained in the present embodiment uses Gaussian function fitting, obtains
Standard variance is 0.104 picosecond, and full width at half maximum is 0.246 picosecond.Experimental data figure is shown in attached drawing 2.
Embodiment 2
Sex-limited energy is detected for the invention integration type femtosecond time resolution fluorescence lifetime measurement spectrometer fluorescence intensity
Investigation, use the 0.1m mol/L ethanol solutions of OX750 dyestuffs as sample；Pump light is 490nm, 1.5mW；Detecting light is
800nm, 1mW.The integration type femtosecond time resolution fluorescence lifetime measurement spectrum of sample is measured, experimental data is shown in attached drawing 3.It is wherein left
Figure (a) is the evolution curve that the OX750 fluorescence spectrums measured change with delay time；Right figure (b) is including glimmering not to OX750
Photopeak does the fluorescence intensity of 660nm unicast strong points that integrates with the attenuation curve (blue filled box) of delay time and right
The integrated fluorescence intensities that OX750 fluorescence peaks obtain after integrating with delay time attenuation curve (red short side frame).It can see
Go out, before the noise of curve is significantly less than integration after integration.This is one and represents the curve that OX750 fluorescence signals generate.
Claims (7)
1. integration type femtosecond time resolution fluorescence lifetime measurement spectrometer, glimmering including detection and pumping laser light path system, sample
Opto-collection system and spectral detection system；It is characterized in that：
The detection and pumping laser light path system include sample cell, time delay device (5) and optical chopper (15), the sample
Product fluorescent collecting system includes sub-ray spectrometer (12), detector (13) and computer (14)；The femtosecond that femto-second laser is sent out
The femtosecond laser that laser acquisition light beam (2) is sent out through time delay device (5) and optical chopper (15) with femtosecond pump laser
Pump beam (3) is exposed on the sample in sample cell (4), and femtosecond laser pump beam (3) makes sample generate fluorescence；Sample
Fluorescence forms multi-color spectrum after sub-ray spectrometer (12) light splitting on detector (13), the multi-color spectrum on detector (13) by
Computer (14) is integrated to obtain fluorescence integrated signal；
After optical chopper (15) is run, no femtosecond laser detection light beam will be obtained on computer (14) and has femtosecond laser
Two fluorescence intensities of light beam are detected to the integrated signal of wavelength, the difference of the two fluorescence integrated signals is exactly fluorescence lifetime signal
Point；It is that ordinate is depicted as curve on computer (14) using delay time as abscissa, fluorescence lifetime signaling point, just obtains sample
The integration type femtosecond time resolution fluorescence lifetime measurement curve of product.
2. integration type femtosecond time resolution fluorescence lifetime measurement spectrometer according to claim 1, it is characterised in that：
All components in detection and pumping laser light path system, fluorescent acquisition system, spectral detection system are all placed in
On optical flat (1).
3. integration type femtosecond time resolution fluorescence lifetime measurement spectrometer according to claim 1, it is characterised in that：
The fluorescent acquisition system is after the fluorescence that sample is radiated is collected by phosphor collection mirror (8), by fluorescent reflection
Mirror (9) is reflected on fluorescent foci mirror (10), is focused on optical fiber (11) by fluorescent foci mirror (10), then from optical fiber (11)
Into sub-ray spectrometer (12)；Phosphor collection mirror (8) and fluorescent foci mirror (10) can be but not limited to lens, paraboloid respectively
Mirror or spherical mirror.
4. integration type femtosecond time resolution fluorescence lifetime measurement spectrometer according to claim 1, it is characterised in that：
The sample cell (4) is the cuvette that light path is 1mm, 2mm or 10mm；Femtosecond laser detection light beam (2) and femtosecond laser
Spatially cross-coincidence of the pump beam (3) in sample cell where sample, femtosecond laser detection light beam (2) is to pass through the time
Delayer (5) adjust and optical chopper (15) after, by delayer back mirror (6) and sample cell front mirror (7),
It is overlapped on sample cell (4) with femtosecond laser pump beam (3) in space crossed.
5. integration type femtosecond time resolution fluorescence lifetime measurement spectrometer according to claim 1, it is characterised in that：
The spectral detection system is fluorescence after sub-ray spectrometer (12) light splitting, and polychrome is formed on detector (13)
It composes, the multi-color spectrum on detector (13) is integrated to obtain fluorescence integrated signal by computer (14)；Detector (13) can be
But it is not limited to photodiode array or charge coupled cell.
6. integration type femtosecond time resolution fluorescence lifetime measurement spectrometer according to claim 1, it is characterised in that：
For the triggering frequency source of the optical chopper (15) in femto-second laser, chopping frequency is set in 20Hz to 1000Hz
Between.
7. the integration type femtosecond time resolution fluorescence lifetime measurement spectrometer according to claim 1 or 6, it is characterised in that：
Computer (14) is connected with each other by data line and time delay device (5), femto-second laser, detector (13).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611101990.3A CN108152252B (en) | 2016-12-05 | 2016-12-05 | Integral femtosecond time resolution fluorescence life measuring spectrometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611101990.3A CN108152252B (en) | 2016-12-05 | 2016-12-05 | Integral femtosecond time resolution fluorescence life measuring spectrometer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108152252A true CN108152252A (en) | 2018-06-12 |
| CN108152252B CN108152252B (en) | 2020-08-18 |
Family
ID=62470640
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611101990.3A Active CN108152252B (en) | 2016-12-05 | 2016-12-05 | Integral femtosecond time resolution fluorescence life measuring spectrometer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108152252B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112285076A (en) * | 2020-09-27 | 2021-01-29 | 浙江谱创仪器有限公司 | Integral type aviation kerosene trace water content measuring molecular fluorescence spectrometer based on DLP technology |
| CN113758939A (en) * | 2020-06-04 | 2021-12-07 | 中国科学院大连化学物理研究所 | Method for representing metal surface cleanliness by using metal surface reflection and scattering spectrum |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002039943A (en) * | 2000-07-27 | 2002-02-06 | Japan Science & Technology Corp | Multiple photon excited fluorescence life time imaging system |
| CN1794079A (en) * | 2005-12-30 | 2006-06-28 | 中国科学院化学研究所 | Femtosecond time resolution fluorescence deficit system |
| US7125518B2 (en) * | 2004-02-14 | 2006-10-24 | The United States Of America As Represented By The Secretary Of The Army | Aerosol particle analyzer for measuring the amount of analyte in airborne particles |
| CN1920535A (en) * | 2006-09-13 | 2007-02-28 | 哈尔滨工程大学 | Device for measuring fluorescence life time |
| CN101632577A (en) * | 2009-08-20 | 2010-01-27 | 浙江大学 | Method and device for detecting enamel mineral substance content based on frequency domain fluorescent service life imaging |
| CN101832931A (en) * | 2010-05-24 | 2010-09-15 | 深圳大学 | Method and system for measuring fluorescence service life |
| CN103592277A (en) * | 2013-11-20 | 2014-02-19 | 中国科学技术大学 | High-precision fluorescent lifetime measuring device |
| CN103868595A (en) * | 2014-03-06 | 2014-06-18 | 湖南大学 | Spatially-separated pump-probe transient absorption spectrograph and realization method |
| CN104422519A (en) * | 2013-09-02 | 2015-03-18 | 中国科学院大连化学物理研究所 | Modularized femtosecond time-resolved transient absorption and fluorescence depletion two-in-one spectrometer |
| CN106124471A (en) * | 2016-07-19 | 2016-11-16 | 天津大学 | Time-domain fluorescent life-span imaging arrangement and life test acquisition methods |
-
2016
- 2016-12-05 CN CN201611101990.3A patent/CN108152252B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002039943A (en) * | 2000-07-27 | 2002-02-06 | Japan Science & Technology Corp | Multiple photon excited fluorescence life time imaging system |
| US7125518B2 (en) * | 2004-02-14 | 2006-10-24 | The United States Of America As Represented By The Secretary Of The Army | Aerosol particle analyzer for measuring the amount of analyte in airborne particles |
| CN1794079A (en) * | 2005-12-30 | 2006-06-28 | 中国科学院化学研究所 | Femtosecond time resolution fluorescence deficit system |
| CN1920535A (en) * | 2006-09-13 | 2007-02-28 | 哈尔滨工程大学 | Device for measuring fluorescence life time |
| CN101632577A (en) * | 2009-08-20 | 2010-01-27 | 浙江大学 | Method and device for detecting enamel mineral substance content based on frequency domain fluorescent service life imaging |
| CN101832931A (en) * | 2010-05-24 | 2010-09-15 | 深圳大学 | Method and system for measuring fluorescence service life |
| CN104422519A (en) * | 2013-09-02 | 2015-03-18 | 中国科学院大连化学物理研究所 | Modularized femtosecond time-resolved transient absorption and fluorescence depletion two-in-one spectrometer |
| CN103592277A (en) * | 2013-11-20 | 2014-02-19 | 中国科学技术大学 | High-precision fluorescent lifetime measuring device |
| CN103868595A (en) * | 2014-03-06 | 2014-06-18 | 湖南大学 | Spatially-separated pump-probe transient absorption spectrograph and realization method |
| CN106124471A (en) * | 2016-07-19 | 2016-11-16 | 天津大学 | Time-domain fluorescent life-span imaging arrangement and life test acquisition methods |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113758939A (en) * | 2020-06-04 | 2021-12-07 | 中国科学院大连化学物理研究所 | Method for representing metal surface cleanliness by using metal surface reflection and scattering spectrum |
| CN113758939B (en) * | 2020-06-04 | 2022-10-18 | 中国科学院大连化学物理研究所 | Method for representing metal surface cleanliness by using metal surface reflection and scattering spectrum |
| CN112285076A (en) * | 2020-09-27 | 2021-01-29 | 浙江谱创仪器有限公司 | Integral type aviation kerosene trace water content measuring molecular fluorescence spectrometer based on DLP technology |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108152252B (en) | 2020-08-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP3553164B1 (en) | Method for inspecting microorganisms, and apparatus for said method | |
| CN100545631C (en) | Multifunctional light absorption, scattering and emission spectrometer based on surface plasma-wave | |
| US9618449B2 (en) | Optical analysis of emissions from stimulated liquids | |
| CN111308289A (en) | Partial discharge multispectral weak light detection device and method | |
| CN111504978B (en) | Pulse type time-delay dispersion spectral measurement method and device and spectral imaging method and device | |
| CN207300890U (en) | For detecting the Raman spectrometer of specific narrow wave-number range | |
| TWI619809B (en) | Method and device for inspecting microorganisms | |
| CN202126403U (en) | Antibiotics analysis device based on resolution of excitation time characteristics of fluorescence spectrum | |
| CN107462566A (en) | For detecting the Raman spectrometer of specific narrow wave-number range | |
| CN103424387A (en) | Fluorescence spectrum detection device for pesticide residue | |
| CN104964964A (en) | Portable laser raman spectrometer based on prismatic decomposition | |
| CN103353440B (en) | Device and method for measuring material diffuse reflectance by adopting gas absorption spectrum | |
| CN108152252A (en) | A kind of integration type femtosecond time resolution fluorescence lifetime measurement spectrometer | |
| CN201273880Y (en) | Apparatus for nondestructively testing doping concentration of laser crystal | |
| CN214097162U (en) | Fluorescence emission spectrum and service life detection system | |
| Prenna et al. | Methodological and instrumentational aspects of cytofluorometry | |
| CN105588826B (en) | A kind of femtosecond time resolution multiple tracks locking phase Fluorescence Spectrometer based on optically erasing | |
| CN211086086U (en) | Device for multiplying signal | |
| CN109991197A (en) | Measure the spectrophotometer and method of fluorescent characteristic | |
| CN209784193U (en) | Equipment capable of measuring Raman spectrum of substance under strong fluorescence background | |
| CN106970058A (en) | The minimal feeding instrument and detection method in a kind of pair of fluorescent emission face | |
| Sen et al. | Phosphorescence lifetime measurements with sensor materials: Comparison of the four different detection platforms | |
| CN203164119U (en) | Two-photon absorption section spectrometer | |
| JP2014042463A (en) | Method of testing microorganism and device thereof | |
| CN206146837U (en) | A optics and detecting system for multichannel atomic fluorescence spectrophotometer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240124 Address after: 116023 No. 457, Zhongshan Road, Liaoning, Dalian Patentee after: DALIAN INSTITUTE OF CHEMICAL PHYSICS, CHINESE ACADEMY OF SCIENCES Country or region after: China Address before: Room 207, Building A, Emerging Industry Development Center, Zhangjiagang Free Trade Zone, Suzhou City, Jiangsu Province Patentee before: ZHANGJIAGANG INDUSTRY TECHNOLOGY RESEARCH INSTITUTE CO.,LTD. DALIAN INSTITUTE OF CHEMICAL PHYSICS CHINESE ACADEMY OF SCIENCES Country or region before: China |
|
| TR01 | Transfer of patent right |