CN101632577A - Method and device for detecting enamel mineral substance content based on frequency domain fluorescent service life imaging - Google Patents
Method and device for detecting enamel mineral substance content based on frequency domain fluorescent service life imaging Download PDFInfo
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Abstract
The invention discloses a method and a device for detecting enamel mineral substance content based on frequency domain fluorescent service life imaging. The method comprises the following steps: projecting light subject to light intensity modulation on a tooth sample; imaging excited fluorescence on a light intensity gain device; and reconstructing a fluorescent service life value by carrying out the common-frequency modulation and the phase adjustment on the gain device according to an algorithm. Because the autologous fluorescent service life of teeth is irrelevant to factors, such as external excited light intensity, detector sensitivity, content of fluorescent group substances, and the like and is just relevant to the factors, such as PH value, oxide ion concentration and calcium ion concentration of a microenvironment in which the fluorescent group substances are located, the internal state of the enamel substance can be objectively described. The invention provides a nondestructive detection method for quantitatively detecting the mineral substance content of teeth, which probably becomes a novel tool for in-vivo real-time observation and quantitative description of the health degree of the teeth and can be widely applied to clinical oral cavity medical diagnosis and the research of a saprodontia pathological change mechanism.
Description
Technical field
The present invention relates to the stomatology field, relate in particular to a kind of method and apparatus that detects based on the enamel mineral substance content of frequency domain fluorescent service life imaging.
Background technology
Adamantinely mainly be made of hydroxyapatite, hydroxyapatite is a kind of fluorophor material, excites down at certain specific wavelength, can produce autofluorescence.Material built-in function information can be characterized by the time resolution characteristics of outgoing fluorescence, under the pH value situation that causes the tooth demineralization less than normal, the autofluorescence life-span of tooth can change, and therefore, promptly can judge the size of tooth mineral matter content according to the size of the fluorescence lifetime of being obtained.
Development with economic society, the enhancing of oral cavity health consciousness, it is objective to need, accurately, harmless tooth mineral matter content detecting method like this, can monitor the dynamic change of tooth mineral matter content at body, change this trend in serious the beginning of demineralization by preventive measure, make content of mineral substances recover normal level.
The detection of traditional tooth mineral matter content need can't be implemented in the measurement of body with tooth slice.In addition, though phenomenon diagnosis tooth demineralization situations such as the relative distribution in the space of with good grounds fluorescence spectrum or fluorescence light intensity, but these methods all with excite factors such as light intensity or detector sensitivity relevant, lack objectivity, false positive or false negative result appear when clinical diagnosis easily.The size of fluorescence lifetime can not be subjected to the influence of extraneous factor, and is only relevant with the residing environment of material, and the phenomenon of photobleaching can not occur.Measurement by the tooth autofluorescence life-span can reflect tooth internal structure and function information, and whether normalization provides reference frame for the clinical judgment content of mineral substances.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, a kind of method and apparatus that detects based on the enamel mineral substance content of frequency domain fluorescent service life imaging is provided.
The method of utilizing frequency domain fluorescent service life imaging to detect tooth mineral matter content is: light source is laser instrument or continuous light source, realize intensity modulation to source emissioning light by internal modulation light intensity modulator or external modulation light intensity modulator, its modulating frequency depends on the autofluorescence life-span of tooth; Behind light modulated process beam expander optical system or the non-imaging optical system, with beam shape incident, place optical filter for continuous light source and obtain excitation wavelength, incide beam splitter, beam reflection is to fluorescence excitation and collect optical system, act on the tooth samples at last, the fluorescence that is inspired is owing to exist action time and quantum efficiency problem, compare optical excitation signal, there is the change of phase contrast and modulation depth in fluorescence signal, its size has definite mathematical relationship with fluorescence lifetime, fluorescence is successively through fluorescence excitation and collection optical system, beam splitter, the fluorescence imaging optical system, be imaged on behind the optical filter on the receiving plane of light intensity multiplier (-icator), by regulating radio-frequency (RF) device and phase-shifter repeatedly with frequently modulating the gain coefficient of light intensity multiplier (-icator) and the initial phase of gain coefficient, on detector, obtain the fluorescence intensity level at a series of outs of phase place, at this, detector with time of integration is equivalent to low-frequency filter, integration is zero to high-frequency signal owing to a plurality of cycles in the time of integration, reconstructs the fluorescence lifetime value by computer according to restructing algorithm at last.
A kind of device that utilizes frequency domain fluorescent service life imaging to detect tooth mineral matter content comprises laser instrument, external modulator, beam expander optical system, beam splitter, fluorescence excitation and collection optical system, tooth samples, the fluorescence imaging optical system, optical filter, high frequency modulated light intensity multiplier (-icator), radio-frequency (RF) device and phase-shifter, detector, computer, order is placed tooth samples successively on same optical axis, fluorescence excitation and collection optical system, beam splitter, the fluorescence imaging optical system, optical filter, high frequency modulated light intensity multiplier (-icator), detector, the light that laser instrument sends enters fluorescence excitation and collects optical system after inciding on the beam splitter through external modulator modulation back by beam expander optical system, act on the tooth samples at last, after the fluorescence that is inspired on the tooth samples enters the fluorescence imaging optical system through fluorescence excitation with after collecting optical system and beam splitter, be imaged on through optical filter on the receiving plane of light intensity multiplier (-icator), repeatedly regulate the gain coefficient of high frequency modulated light intensity multiplier (-icator) and the initial phase of gain coefficient by regulating radio-frequency (RF) device and phase-shifter, the fluorescence intensity level at a series of outs of phase place that obtains on detector goes out the fluorescence lifetime value by computer reconstruction.
A kind of device that utilizes frequency domain fluorescent service life imaging to detect tooth mineral matter content comprises laser instrument, internal modulator, beam expander optical system, beam splitter, fluorescence excitation and collection optical system, tooth samples, the fluorescence imaging optical system, optical filter, high frequency modulated light intensity multiplier (-icator), radio-frequency (RF) device and phase-shifter, detector, computer, order is placed tooth samples successively on same optical axis, fluorescence excitation and collection optical system, beam splitter, the fluorescence imaging optical system, optical filter, high frequency modulated light intensity multiplier (-icator), detector, the light that laser instrument sends enters fluorescence excitation and collects optical system after inciding on the beam splitter through internal modulator modulation back by beam expander optical system, act on the tooth samples at last, after the fluorescence that is inspired on the tooth samples enters the fluorescence imaging optical system through fluorescence excitation with after collecting optical system and beam splitter, be imaged on through optical filter on the receiving plane of light intensity multiplier (-icator), repeatedly regulate the gain coefficient of high frequency modulated light intensity multiplier (-icator) and the initial phase of gain coefficient by regulating radio-frequency (RF) device and phase-shifter, the fluorescence intensity level at a series of outs of phase place that obtains on detector goes out the fluorescence lifetime value by computer reconstruction.
A kind of device that utilizes frequency domain fluorescent service life imaging to detect tooth mineral matter content comprises continuous light source, internal modulator, non-imaging optical system, optical filter, beam splitter, fluorescence excitation and collection optical system, tooth samples, the fluorescence imaging optical system, optical filter, high frequency modulated light intensity multiplier (-icator), radio-frequency (RF) device and phase-shifter, detector, computer, order is placed tooth samples successively on same optical axis, fluorescence excitation and collection optical system, beam splitter, the fluorescence imaging optical system, optical filter, high frequency modulated light intensity multiplier (-icator), detector, continuous light source enters fluorescence excitation and collects optical system after inciding on the beam splitter through internal modulator modulation back by non-imaging system and optical filter, act on the tooth samples at last, after the fluorescence that is inspired on the tooth samples enters the fluorescence imaging optical system through fluorescence excitation with after collecting optical system and beam splitter, be imaged on through optical filter on the receiving plane of light intensity multiplier (-icator), repeatedly regulate the gain coefficient of high frequency modulated light intensity multiplier (-icator) and the initial phase of gain coefficient by regulating radio-frequency (RF) device and phase-shifter, the fluorescence intensity level at a series of outs of phase place that obtains on detector goes out the fluorescence lifetime value by computer reconstruction.
A kind of device that utilizes frequency domain fluorescent service life imaging to detect tooth mineral matter content comprises continuous light source, external modulator, non-imaging optical system, optical filter, beam splitter, fluorescence excitation and collection optical system, tooth samples, the fluorescence imaging optical system, optical filter, high frequency modulated light intensity multiplier (-icator), radio-frequency (RF) device and phase-shifter, detector, computer, order is placed tooth samples successively on same optical axis, fluorescence excitation and collection optical system, beam splitter, the fluorescence imaging optical system, optical filter, high frequency modulated light intensity multiplier (-icator), detector, the light that continuous light source sends is modulated the back by entering fluorescence excitation after inciding on the beam splitter behind non-imaging optical system and the optical filter and collecting optical system through external modulator, act on the tooth samples at last, after the fluorescence that is inspired on the tooth samples enters the fluorescence imaging optical system through fluorescence excitation with after collecting optical system and beam splitter, be imaged on through optical filter on the receiving plane of light intensity multiplier (-icator), repeatedly regulate the gain coefficient of high frequency modulated light intensity multiplier (-icator) and the initial phase of gain coefficient by regulating radio-frequency (RF) device and phase-shifter, the fluorescence intensity level at a series of outs of phase place that obtains on detector goes out the fluorescence lifetime value by computer reconstruction.
But the beneficial effect that the present invention compared with prior art has is the detection that can be implemented in the tooth mineral matter content of the harmless quantification of body, dynamic monitoring by optical means, with fluorescence lifetime as the parameter of judging tooth mineral matter content, set up the quantitative relationship of the two, for clinical oral cavity doctor provides diagnosis criterion to the tooth health situation, has application potential in clinical stomatology field.
Description of drawings
Fig. 1 external modulation LASER Light Source wide field frequency domain fluorescent service life imaging measuring device sketch map;
Fig. 2 internal modulation LASER Light Source wide field frequency domain fluorescent service life imaging measuring device sketch map;
Fig. 3 internal modulation continuous light source wide field frequency domain fluorescent service life imaging measuring device sketch map;
Fig. 4 external modulation continuous light source wide field frequency domain fluorescent service life imaging measuring device sketch map;
Among the figure, laser instrument 1, external modulator 2, beam expander optical system 3, beam splitter 4, fluorescence excitation and collection optical system 5, tooth samples 6, fluorescence imaging optical system 7, optical filter 8, high frequency modulated light intensity multiplier (-icator) 9, radio-frequency (RF) device and phase-shifter 10, detector 11, computer 12, internal modulator 13, continuous light source 14, optical filter 15, non-imaging optical system 16.
The specific embodiment
The method of utilizing frequency domain fluorescent service life imaging to detect tooth mineral matter content is that light source is laser instrument or continuous light source, realize intensity modulation to source emissioning light by internal modulation light intensity modulator or external modulation light intensity modulator, its modulating frequency depends on the autofluorescence life-span of tooth; Behind light modulated process beam expander optical system or the non-imaging optical system, with beam shape incident, place optical filter for continuous light source and obtain excitation wavelength, incide beam splitter, beam reflection is to fluorescence excitation and collect optical system, act on the tooth samples at last, the fluorescence that is inspired is owing to exist action time and quantum efficiency problem, compare optical excitation signal, there is the change of phase contrast and modulation depth in fluorescence signal, its size has definite mathematical relationship with fluorescence lifetime, fluorescence is successively through fluorescence excitation and collection optical system, beam splitter, fluorescence imaging optical system, be imaged on the receiving plane of light intensity multiplier (-icator) the receiving plane phase conjugate of the shadow surface of tooth and light intensity multiplier (-icator) behind the optical filter.By regulating radio-frequency (RF) device and phase-shifter repeatedly with frequently modulating the gain coefficient of light intensity multiplier (-icator) and the initial phase of gain coefficient, on detector, obtain the fluorescence intensity level at a series of outs of phase place, at this, detector with time of integration is equivalent to low-frequency filter, integration is zero to high-frequency signal owing to a plurality of cycles in the time of integration, reconstructs the fluorescence lifetime value by computer according to restructing algorithm at last.And different fluorescence lifetime sizes is marked by pseudo-colours.By detection, can judge the content of mineral substances situation of tooth samples to the fluorescence lifetime value of sample to be tested two-dimensional space.
As shown in Figure 1, a kind of device that utilizes frequency domain fluorescent service life imaging to detect tooth mineral matter content comprises laser instrument 1, external modulator 2, beam expander optical system 3, beam splitter 4, fluorescence excitation and collection optical system 5, tooth samples 6, fluorescence imaging optical system 7, optical filter 8, high frequency modulated light intensity multiplier (-icator) 9, radio-frequency (RF) device and phase-shifter 10, detector 11, computer 12, order is placed tooth samples 6 successively on same optical axis, fluorescence excitation and collection optical system 5, beam splitter 4, fluorescence imaging optical system 7, optical filter 8, high frequency modulated light intensity multiplier (-icator) 9, detector 11, the light that laser instrument 1 sends enters fluorescence excitation and collects optical system 5 after inciding on the beam splitter 4 through external modulator 2 modulation backs by beam expander optical system 3, act at last on the tooth samples 6, after the fluorescence that is inspired on the tooth samples 6 enters fluorescence imaging optical system 7 through fluorescence excitation with after collecting optical system 5 and beam splitter 4, be imaged on through optical filter 8 on the receiving plane of light intensity multiplier (-icator) 9, regulate the gain coefficient of high frequency modulated light intensity multiplier (-icator) 9 and the initial phase of gain coefficient for more than 10 time by regulating radio-frequency (RF) device and phase-shifter, the fluorescence intensity level at a series of outs of phase place that obtains on detector 11 reconstructs the fluorescence lifetime value by computer 12.
As shown in Figure 2, a kind of device that utilizes frequency domain fluorescent service life imaging to detect tooth mineral matter content comprises laser instrument 1, internal modulator 13, beam expander optical system 3, beam splitter 4, fluorescence excitation and collection optical system 5, tooth samples 6, fluorescence imaging optical system 7, optical filter 8, high frequency modulated light intensity multiplier (-icator) 9, radio-frequency (RF) device and phase-shifter 10, detector 11, computer 12, order is placed tooth samples 6 successively on same optical axis, fluorescence excitation and collection optical system 5, beam splitter 4, fluorescence imaging optical system 7, optical filter 8, high frequency modulated light intensity multiplier (-icator) 9, detector 11, the light that laser instrument 1 sends enters fluorescence excitation and collects optical system 5 after inciding on the beam splitter 4 through internal modulator 13 modulation backs by beam expander optical system 3, act at last on the tooth samples 6, after the fluorescence that is inspired on the tooth samples 6 enters fluorescence imaging optical system 7 through fluorescence excitation with after collecting optical system 5 and beam splitter 4, be imaged on through optical filter 8 on the receiving plane of light intensity multiplier (-icator) 9, regulate the gain coefficient of high frequency modulated light intensity multiplier (-icator) 9 and the initial phase of gain coefficient for more than 10 time by regulating radio-frequency (RF) device and phase-shifter, the fluorescence intensity level at a series of outs of phase place that obtains on detector 11 reconstructs the fluorescence lifetime value by computer 12.
As shown in Figure 3, a kind of device that utilizes frequency domain fluorescent service life imaging to detect tooth mineral matter content comprises continuous light source 14, internal modulator 13, non-imaging optical system 16, optical filter 15, beam splitter 4, fluorescence excitation and collection optical system 5, tooth samples 6, fluorescence imaging optical system 7, optical filter 8, high frequency modulated light intensity multiplier (-icator) 9, radio-frequency (RF) device and phase-shifter 10, detector 11, computer 12, order is placed tooth samples 6 successively on same optical axis, fluorescence excitation and collection optical system 5, beam splitter 4, fluorescence imaging optical system 7, optical filter 8, high frequency modulated light intensity multiplier (-icator) 9, detector 11, continuous light source 14 enters fluorescence excitation and collects optical system 5 after inciding on the beam splitter 4 through internal modulator 13 modulation backs by non-imaging system 16 and optical filter 15, act at last on the tooth samples 6, after the fluorescence that is inspired on the tooth samples 6 enters fluorescence imaging optical system 7 through fluorescence excitation with after collecting optical system 5 and beam splitter 4, be imaged on through optical filter 8 on the receiving plane of light intensity multiplier (-icator) 9, regulate the gain coefficient of high frequency modulated light intensity multiplier (-icator) 9 and the initial phase of gain coefficient for more than 10 time by regulating radio-frequency (RF) device and phase-shifter, the fluorescence intensity level at a series of outs of phase place that obtains on detector 11 reconstructs the fluorescence lifetime value by computer 12.
As shown in Figure 4, a kind of device that utilizes frequency domain fluorescent service life imaging to detect tooth mineral matter content comprises continuous light source 14, external modulator 2, non-imaging optical system 16, optical filter 15, beam splitter 4, fluorescence excitation and collection optical system 5, tooth samples 6, fluorescence imaging optical system 7, optical filter 8, high frequency modulated light intensity multiplier (-icator) 9, radio-frequency (RF) device and phase-shifter 10, detector 11, computer 12, order is placed tooth samples 6 successively on same optical axis, fluorescence excitation and collection optical system 5, beam splitter 4, fluorescence imaging optical system 7, optical filter 8, high frequency modulated light intensity multiplier (-icator) 9, detector 11, the light that continuous light source 14 sends is modulated the back by entering fluorescence excitation after inciding on the beam splitter 4 behind non-imaging optical system 16 and the optical filter 15 and collecting optical system 5 through external modulator 2, act at last on the tooth samples 6, after the fluorescence that is inspired on the tooth samples 6 enters fluorescence imaging optical system 7 through fluorescence excitation with after collecting optical system 5 and beam splitter 4, be imaged on through optical filter 8 on the receiving plane of light intensity multiplier (-icator) 9, regulate the gain coefficient of high frequency modulated light intensity multiplier (-icator) 9 and the initial phase of gain coefficient for more than 10 time by regulating radio-frequency (RF) device and phase-shifter, the fluorescence intensity level at a series of outs of phase place that obtains on detector 11 reconstructs the fluorescence lifetime value by computer 12.
The present invention adopts laser instrument such as solid state laser or semiconductor laser to be applied to the excitation source of tooth mineral matter content detection as frequency domain fluorescent service life imaging, the size of power depends on the needs of tooth autofluorescence emission, the selection of wavelength is chosen the excitation wavelength of outgoing fluorescence intensity maximum according to the excitation spectrum test of tooth.Adopt continuous light source, as LED, xenon lamp, deuterium lamp etc. utilize the non-imaging optical system design to collect emission luminous energy as light source, reach the energy and the shape need of incident illumination.Take the high frequency modulated of the method realization light intensity of external modulation, realize that the device of high frequency external modulation has electrooptic modulator, acousto-optic modulator, magneto-optic modulator etc.The size of intensity modulation frequency depends on the size of test substance fluorescence lifetime.Adopt beam expander optical system to make the laser facula of high frequency modulated satisfy the spot size that incides on the sample.Adopt beam splitter that incident illumination is reflexed on the sample to be tested, and the fluorescence of outgoing is transmitted to follow-up fluorescence receiving system.Adopt fluorescence excitation and collect optical system the exciting light wide field is acted on tooth samples, and collect the fluorescence signal of outgoing simultaneously.Employing is placed the method for optical filter and is got rid of exciting light and external environmental interference factor before detector, only obtain particular fluorescent signal.Adopt the light intensity multiplier (-icator) of high frequency response to realize the light intensity of fluorescent signals is gained.Adopt radio-frequency (RF) device and phase shifter to realize to the high frequency modulated of light intensity multiplier (-icator) gain coefficient and the change of initial phase.Adopt the aerial image of realizations such as photodetector CCD or CMOS to light intensity.
Claims (5)
1. method of utilizing frequency domain fluorescent service life imaging to detect tooth mineral matter content, it is characterized in that light source is laser instrument or continuous light source, realize intensity modulation to source emissioning light by internal modulation light intensity modulator or external modulation light intensity modulator, its modulating frequency depends on the autofluorescence life-span of tooth; Behind light modulated process beam expander optical system or the non-imaging optical system, with beam shape incident, place optical filter for continuous light source and obtain excitation wavelength, incide beam splitter, beam reflection is to fluorescence excitation and collect optical system, act on the tooth samples at last, the fluorescence that is inspired is owing to exist action time and quantum efficiency problem, compare optical excitation signal, there is the change of phase contrast and modulation depth in fluorescence signal, its size has definite mathematical relationship with fluorescence lifetime, fluorescence is successively through fluorescence excitation and collection optical system, beam splitter, the fluorescence imaging optical system, be imaged on behind the optical filter on the receiving plane of light intensity multiplier (-icator), by regulating radio-frequency (RF) device and phase-shifter repeatedly with frequently modulating the gain coefficient of light intensity multiplier (-icator) and the initial phase of gain coefficient, on detector, obtain the fluorescence intensity level at a series of outs of phase place, at this, detector with time of integration is equivalent to low-frequency filter, integration is zero to high-frequency signal owing to a plurality of cycles in the time of integration, reconstructs the fluorescence lifetime value by computer according to restructing algorithm at last.
2. device that utilizes frequency domain fluorescent service life imaging to detect tooth mineral matter content, it is characterized in that comprising laser instrument (1), external modulator (2), beam expander optical system (3), beam splitter (4), fluorescence excitation and collection optical system (5), tooth samples (6), fluorescence imaging optical system (7), optical filter (8), high frequency modulated light intensity multiplier (-icator) (9), radio-frequency (RF) device and phase-shifter (10), detector (11), computer (12), order is placed tooth samples (6) successively on same optical axis, fluorescence excitation and collection optical system (5), beam splitter (4), fluorescence imaging optical system (7), optical filter (8), high frequency modulated light intensity multiplier (-icator) (9), detector (11), the light that laser instrument (1) sends incides by beam expander optical system (3) through external modulator (2) modulation back and enters fluorescence excitation after beam splitter (4) is gone up and collect optical system (5), act at last on the tooth samples (6), after the fluorescence that is inspired on the tooth samples (6) enters fluorescence imaging optical system (7) through fluorescence excitation with after collecting optical system (5) and beam splitter (4), be imaged on the receiving plane of light intensity multiplier (-icator) (9) through optical filter (8), repeatedly regulate the gain coefficient of high frequency modulated light intensity multiplier (-icator) (9) and the initial phase of gain coefficient by regulating radio-frequency (RF) device and phase-shifter (10), the fluorescence intensity level at a series of outs of phase place that obtains on detector (11) reconstructs the fluorescence lifetime value by computer (12).
3. device that utilizes frequency domain fluorescent service life imaging to detect tooth mineral matter content, it is characterized in that comprising laser instrument (1), internal modulator (13), beam expander optical system (3), beam splitter (4), fluorescence excitation and collection optical system (5), tooth samples (6), fluorescence imaging optical system (7), optical filter (8), high frequency modulated light intensity multiplier (-icator) (9), radio-frequency (RF) device and phase-shifter (10), detector (11), computer (12), order is placed tooth samples (6) successively on same optical axis, fluorescence excitation and collection optical system (5), beam splitter (4), fluorescence imaging optical system (7), optical filter (8), high frequency modulated light intensity multiplier (-icator) (9), detector (11), the light that laser instrument (1) sends incides by beam expander optical system (3) through internal modulator (13) modulation back and enters fluorescence excitation after beam splitter (4) is gone up and collect optical system (5), act at last on the tooth samples (6), after the fluorescence that is inspired on the tooth samples (6) enters fluorescence imaging optical system (7) through fluorescence excitation with after collecting optical system (5) and beam splitter (4), be imaged on the receiving plane of light intensity multiplier (-icator) (9) through optical filter (8), repeatedly regulate the gain coefficient of high frequency modulated light intensity multiplier (-icator) (9) and the initial phase of gain coefficient by regulating radio-frequency (RF) device and phase-shifter (10), the fluorescence intensity level at a series of outs of phase place that obtains on detector (11) reconstructs the fluorescence lifetime value by computer (12).
4. device that utilizes frequency domain fluorescent service life imaging to detect tooth mineral matter content, it is characterized in that comprising continuous light source (14), internal modulator (13), non-imaging optical system (16), optical filter (15), beam splitter (4), fluorescence excitation and collection optical system (5), tooth samples (6), fluorescence imaging optical system (7), optical filter (8), high frequency modulated light intensity multiplier (-icator) (9), radio-frequency (RF) device and phase-shifter (10), detector (11), computer (12), order is placed tooth samples (6) successively on same optical axis, fluorescence excitation and collection optical system (5), beam splitter (4), fluorescence imaging optical system (7), optical filter (8), high frequency modulated light intensity multiplier (-icator) (9), detector (11), continuous light source (14) incides by non-imaging system (16) and optical filter (15) through internal modulator (13) modulation back and enters fluorescence excitation after beam splitter (4) is gone up and collect optical system (5), act at last on the tooth samples (6), after the fluorescence that is inspired on the tooth samples (6) enters fluorescence imaging optical system (7) through fluorescence excitation with after collecting optical system (5) and beam splitter (4), be imaged on the receiving plane of light intensity multiplier (-icator) (9) through optical filter (8), repeatedly regulate the gain coefficient of high frequency modulated light intensity multiplier (-icator) (9) and the initial phase of gain coefficient by regulating radio-frequency (RF) device and phase-shifter (10), the fluorescence intensity level at a series of outs of phase place that obtains on detector (11) reconstructs the fluorescence lifetime value by computer (12).
5. device that utilizes frequency domain fluorescent service life imaging to detect tooth mineral matter content, it is characterized in that comprising continuous light source (14), external modulator (2), non-imaging optical system (16), optical filter (15), beam splitter (4), fluorescence excitation and collection optical system (5), tooth samples (6), fluorescence imaging optical system (7), optical filter (8), high frequency modulated light intensity multiplier (-icator) (9), radio-frequency (RF) device and phase-shifter (10), detector (11), computer (12), order is placed tooth samples (6) successively on same optical axis, fluorescence excitation and collection optical system (5), beam splitter (4), fluorescence imaging optical system (7), optical filter (8), high frequency modulated light intensity multiplier (-icator) (9), detector (11), the light that continuous light source (14) sends enters fluorescence excitation after beam splitter (4) is gone up and collects optical system (5) by inciding behind non-imaging optical system (16) and the optical filter (15) through external modulator (2) modulation back, act on tooth samples (6) at last, at the fluorescence that inspired on the tooth samples (6) through fluorescence excitation with collect optical system (5) and after beam splitter (4) enters fluorescence imaging optical system (7), be imaged on the receiving plane of light intensity multiplier (-icator) (9) through optical filter (8), repeatedly regulate the gain coefficient of high frequency modulated light intensity multiplier (-icator) (9) and the initial phase of gain coefficient by regulating radio-frequency (RF) device and phase-shifter (10), the fluorescence intensity level at a series of outs of phase place that obtains on detector (11) reconstructs the fluorescence lifetime value by computer (12).
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| CN101915754A (en) * | 2010-05-19 | 2010-12-15 | 上海理工大学 | Double-path frequency-division multiplexing fluorescent confocal microscopic imaging system and implementation method |
| CN103163106A (en) * | 2013-01-30 | 2013-06-19 | 浙江大学 | Super-resolution fluorescent lifetime imaging method and device based on stimulated emission lost |
| CN107850531A (en) * | 2015-05-28 | 2018-03-27 | 捷普有限公司 | Systems, devices and methods for the jointing material inspection of distribution |
| CN108152252A (en) * | 2016-12-05 | 2018-06-12 | 中科院大连化学物理研究所张家港产业技术研究院有限公司 | A kind of integration type femtosecond time resolution fluorescence lifetime measurement spectrometer |
| CN108344738A (en) * | 2018-01-22 | 2018-07-31 | 翰飞骏德（北京）医疗科技有限公司 | Imaging method and its device for hydroxyapatite |
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| JP4459390B2 (en) * | 2000-06-08 | 2010-04-28 | 浜松ホトニクス株式会社 | Fluorescence measurement method, fluorescence measurement apparatus, and sample evaluation apparatus using the same |
| US7577284B2 (en) * | 2006-04-21 | 2009-08-18 | Carestream Health, Inc. | Optical detection of dental caries |
| CN201252656Y (en) * | 2008-01-17 | 2009-06-03 | 大连理工大学 | Time-domain-method-based universal photo-electric device for measuring the service life of the fluorescence |
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| CN101915754A (en) * | 2010-05-19 | 2010-12-15 | 上海理工大学 | Double-path frequency-division multiplexing fluorescent confocal microscopic imaging system and implementation method |
| CN103163106A (en) * | 2013-01-30 | 2013-06-19 | 浙江大学 | Super-resolution fluorescent lifetime imaging method and device based on stimulated emission lost |
| CN103163106B (en) * | 2013-01-30 | 2014-12-17 | 浙江大学 | Super-resolution fluorescent lifetime imaging method and device based on stimulated emission lost |
| CN107850531A (en) * | 2015-05-28 | 2018-03-27 | 捷普有限公司 | Systems, devices and methods for the jointing material inspection of distribution |
| CN108152252A (en) * | 2016-12-05 | 2018-06-12 | 中科院大连化学物理研究所张家港产业技术研究院有限公司 | A kind of integration type femtosecond time resolution fluorescence lifetime measurement spectrometer |
| CN108152252B (en) * | 2016-12-05 | 2020-08-18 | 中科院大连化学物理研究所张家港产业技术研究院有限公司 | Integral femtosecond time resolution fluorescence life measuring spectrometer |
| CN108344738A (en) * | 2018-01-22 | 2018-07-31 | 翰飞骏德（北京）医疗科技有限公司 | Imaging method and its device for hydroxyapatite |
| CN112161946A (en) * | 2020-08-28 | 2021-01-01 | 复旦大学 | Frequency domain luminous life imaging system |
| CN112161946B (en) * | 2020-08-28 | 2022-04-15 | 复旦大学 | Frequency domain luminous life imaging system |
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