CN115607110B - Mammary gland tumor detection system based on autofluorescence - Google Patents
Mammary gland tumor detection system based on autofluorescence Download PDFInfo
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- 208000026310 Breast neoplasm Diseases 0.000 claims abstract description 28
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- 206010006187 Breast cancer Diseases 0.000 claims abstract description 27
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
- A61B5/0091—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for mammography
Abstract
The invention relates to a breast tumor detection system based on autofluorescence. The system comprises an excitation light source, a light conduction module light conduction unit, a transient detection unit and a data acquisition and processing unit; wherein the excitation light source is used for generating excitation light; the light conduction unit transmits the excitation light to irradiate the tissue to be detected, and an autofluorescence signal generated by excitation of the tissue to be detected is transmitted to the transient detection unit through the light conduction unit; the transient detection module comprises a high-speed response detector which converts fluorescent signals into electric signals; the data acquisition processing unit comprises a data acquisition device and a fluorescence lifetime fitting module, wherein the data acquisition device converts an electric signal output by the high-speed response detector into a digital signal; and the fluorescence fitting module carries out convolution fitting processing on the digital signals to obtain the fluorescence lifetime of the self fluorescence signal. The breast tumor detection system has the advantages of low cost and small volume.
Description
Technical Field
The invention relates to the field of medical detection, in particular to the field of biological tissue spectrum detection.
Background
Fluorescence detection methods are one of the methods for discriminating tumor tissues, which have been continuously developed in recent years, and can be classified into an autofluorescence detection method and a fluorescence probe detection method. The autofluorescence method is to apply excitation light with specific wavelength to biological tissue to excite endogenous fluorescent radical molecule to enter excited state, and to release photon back to ground state finally through radiation relaxation process. Since the tissue contains a plurality of endogenous fluorescent groups, the ratio and the fluorescence characteristics of the endogenous fluorescent groups in normal tissue and tumor tissue are different, and therefore, the normal tissue and the tumor tissue can be discriminated and judged by detecting the fluorescence spectrum or the fluorescence lifetime of the autofluorescence generated by the tissue.
However, since the endogenous fluorescent groups of the tissue are complex and various, the autofluorescence obtained by the same tissue under the excitation light with different wavelengths has a large difference in spectral characteristic peak distribution and fluorescence lifetime, so that determining the appropriate excitation light wavelength for a specific tissue is important for an autofluorescence method. At present, a few reports are made on screening tumor tissues by adopting an autofluorescence method, but less researches are carried out on fluorescence detection of breast tumors.
In addition, because the fluorescence lifetime of the tissue itself is short, high-resolution transient measurement is often required in the existing high-performance fluorescence spectrum system to obtain a more accurate fluorescence lifetime. However, the method needs to use picosecond-level or femtosecond-level laser as a light source and uses time-related single photon counter or pump-detection technology to measure, so that the whole system needs to consume huge cost, has relatively large volume, is difficult to popularize and apply, and is difficult to adapt to mobile measurement.
Disclosure of Invention
The present invention is based on the object of providing a breast tumor detection system based on autofluorescence, which is low in cost, small in size and specifically applicable to the screening detection of breast tumor tissues.
The breast tumor detection system based on autofluorescence comprises an excitation light source, a light conduction unit, a transient detection unit and a data acquisition and processing unit; wherein the excitation light source generates ultraviolet excitation light with a pulse width of hundred picoseconds and a repetition frequency of kilohertz; the light conduction unit transmits the excitation light to irradiate the tissue to be detected, and an autofluorescence signal generated by excitation of the tissue to be detected is transmitted to the transient detection unit through the light conduction unit; the transient detection unit comprises a high-speed response detector with response time at sub-nanosecond level, and the high-speed response detector converts a fluorescent signal returned by the light conduction unit into an electric signal; the data acquisition processing unit comprises a data acquisition unit and a fluorescence lifetime fitting module, wherein the bandwidth of the data acquisition unit is at least 1GHz, the sampling rate is at least 4GS/s, the storage depth is at least 1Mpts, and the data acquisition processing unit converts the electric signals output by the high-speed response detector into digital signals; and the fluorescence fitting module carries out convolution fitting processing on the digital signals to obtain the fluorescence lifetime of the self fluorescence signal.
The mammary gland tumor detection system of the invention realizes accurate acquisition and calculation of fluorescence lifetime by using a lower-cost and miniaturized facility through the combination of the excitation light source, the high-speed response detector, the data acquisition device and the convolution fitting treatment, and has the advantages of low cost, good effect and easy popularization.
Further, the formula of the convolution fitting processing in the detection system is as follows:
wherein the method comprises the steps of
Wherein, t and t' are both time, B is the normalization factor of Gaussian response function, w is the response characteristic time of the system, τ r For the fluorescence rising edge characteristic time, A 0 And t 0 The coordinate translation amounts of fluorescence intensity and time respectively, k is the number of fluorescence attenuation processes, A i And τ i The amplitude and the service life of the corresponding fluorescence attenuation process are respectively;
the average fluorescence lifetime τ is calculated from the following formula:
further, the excitation light source in the detection system comprises a laser and a collimating mirror, wherein the laser is used for generating laser, and the collimating mirror is arranged on the light path of the laser.
Further, the light conduction unit in the detection system comprises a dichroic mirror, a first optical fiber coupling mirror, an optical fiber and a second optical fiber coupling mirror, wherein the dichroic mirror has high reflectivity for excitation light and high transmissivity for autofluorescence, is arranged on an optical path of the excitation light and reflects the excitation light into the first optical fiber coupling mirror, and two ends of the optical fiber are respectively connected with the first optical fiber coupling mirror and the second optical fiber coupling mirror.
Further, the collimating mirror, the first optical fiber coupling mirror and the second optical fiber coupling mirror in the detection system are quartz lenses, and the optical fibers are ultraviolet light fibers.
Further, the detection system also comprises a spectroscope and a steady state detection unit, wherein the spectroscope is arranged on an optical path of the self-fluorescence signal output by the light conduction unit, and is used for dividing the fluorescence signal into a transient state path and a steady state path, and enabling the fluorescence signal of the transient state path to enter the transient state detection unit, and enabling the fluorescence signal of the steady state path to enter the steady state detection unit; the steady state detection unit comprises a fluorescent signal multichannel band-pass filter device and a high-sensitivity detector which are sequentially arranged on a steady state road, wherein the multichannel band-pass filter device comprises at least two filter channels, and each filter channel can enable fluorescent signals in a specific wavelength range to pass through; the high-sensitivity detector respectively converts the light intensity of fluorescent signals passing through different filtering channels into electric signals; and the data acquisition device also integrates and acquires the electric signals output by the high-sensitivity detector to obtain fluorescent signal intensity values in different wavelength ranges.
Further, the multichannel band-pass filter device in the detection system comprises four filter channels, the bandwidth of the four filter channels is 10nm, and the central wavelengths of the four filter channels are 400nm, 420nm, 430nm and 465nm respectively.
Further, the multichannel band-pass filter device in the detection system is a roller type band-pass filter group, and comprises a roller frame and 10nm band-pass filters with center wavelengths of 400nm, 420nm, 430nm and 465nm respectively.
Further, the excitation light wavelength in the detection system is 355nm.
Further, the data acquisition processing unit in the detection system further comprises a light intensity value processing module, wherein the light intensity value processing module is used for processing the fluorescent signal intensity values in different wavelength ranges obtained by the data acquisition device according to the formula (I) 2 +I 3 )/(I 1 +I 4 ) Performing operation, wherein I 1 、I 2 、I 3 、I 4 The fluorescence signal intensity values passing through the filter channels with the center wavelengths of 400nm, 420nm, 430nm and 465nm are sequentially given.
For a better understanding and implementation, the present invention is described in detail below with reference to the drawings.
Drawings
FIG. 1 is a schematic diagram of a breast tumor detection system according to the present invention;
FIG. 2 is a schematic diagram of a breast tumor detection system according to the present invention;
FIG. 3 is a graph showing the fluorescence lifetime detection results of breast tumor tissue and breast tissue according to the example;
FIG. 4 is a graph showing the results of fluorescence intensity measurements for four characteristic wavelengths of breast tumor tissue and breast tissue according to the example;
fig. 5 is a schematic diagram showing the results of collecting fluorescence intensities of four characteristic wavelengths and calculating R values for breast tumor tissue and breast tissue, respectively, according to the embodiment.
Detailed Description
Aiming at the problems of high cost and huge volume of the whole detection system caused by the fact that the fluorescence service life can be accurately measured only by combining picosecond or femtosecond excitation light sources with measurement means such as a time-related single photon counter or pumping-detection in the prior art, the invention provides a system capable of detecting the fluorescence service life by using hundred picosecond excitation light sources and a subnanosecond detector. The influence of the response broadening of the system on the result is removed by performing post-processing on the data by using a convolution fitting method, so that the system can be ensured to obtain accurate fluorescence lifetime, and the manufacturing cost and the whole volume of the system are greatly reduced. The following is a specific embodiment of the breast tumor detection system according to the present invention.
Referring to fig. 1 and fig. 2, a schematic frame diagram and a schematic structure diagram of a breast tumor detection system according to the present embodiment include an excitation light source 1, a light conduction unit 2, a spectroscope 3, a transient detection unit 4, a steady detection unit 5, and a data acquisition processing unit 7.
The excitation light source 1 comprises a laser 11, an attenuator 12 and a collimator mirror 13. Wherein the laser 11 generates an ultraviolet laser having a wavelength of 355nm, a pulse width of the order of hundred picoseconds and a repetition rate of the order of kilohertz. Preferably, the laser is a microchip solid-state pulse laser. The attenuator 12 and the collimator lens 13 are sequentially disposed on the optical path of the laser light. Wherein the attenuator 12 is in particular a tunable optical attenuator for attenuating the power of the laser light so as to obtain a suitable excitation light, in this embodiment the power of the excitation light is preferably 2mW. The collimating lens 13 is used for collimating the excitation light, and in the testing process, the focal length parameters of the collimating lens can be adjusted according to actual needs to obtain different excitation light beam diameters, preferably, the collimating lens is a quartz lens, and the influence on the detection result caused by fluorescence excited by ultraviolet laser of glass can be avoided by using the quartz lens.
The light conduction unit 2 includes a dichroic mirror 21 and a light guiding module, where the dichroic mirror 21 has a high reflectivity to the excitation light, and the fluorescence signal generated by the tissue to be inspected has a high transmissivity, and is disposed on the optical path of the excitation light, so that the excitation light generated by the excitation light source can be reflected into the light guiding module, and the light guiding module can make the light signal be transmitted bidirectionally. Specifically, in this embodiment, the light guide module includes a first optical fiber coupling mirror 22, an optical fiber 23, and a second optical fiber coupling mirror 24, and two ends of the optical fiber 23 are respectively connected to the first optical fiber coupling mirror 22 and the second optical fiber coupling mirror 24. The excitation light is reflected by the dichroic mirror, enters the first optical fiber coupling mirror 22, is coupled into the optical fiber 23, and irradiates the tissue 6 to be detected through the second optical fiber coupling mirror 24, and excites the tissue. The self-fluorescence signal generated after the tissue 7 to be detected is excited enters the optical fiber 23 through the second optical fiber coupling mirror 24 and exits to the dichroic mirror 21 after passing through the first optical fiber coupling mirror 22. Preferably, the first fiber coupling mirror 22 and the second fiber coupling mirror 24 use quartz lenses, and the optical fiber 23 is an ultraviolet fiber. Since the dichroic mirror 21 has high reflectivity to the excitation light and has high transmissivity to the fluorescence signal generated by the tissue 6 to be inspected, the self-fluorescence signal outputted from the first optical fiber coupling mirror 22 may pass through the dichroic mirror 21, and the scattered excitation light entrained therein may be reflected. And the quartz lens is used as an optical fiber coupling mirror, and the ultraviolet light fiber is used, so that the influence on a detection result caused by fluorescence excited by ultraviolet laser of glass can be avoided. In other embodiments, the light guide module may also be composed of an open lens, so long as excitation light and autofluorescence signals can be transmitted along the light guide module.
The beam splitter 3 may split the incident light into two beams, which are disposed on the optical path of the autofluorescence signal passing through the dichroic mirror 21, for splitting the autofluorescence signal into a transient path and a steady-state path, and making the transient path enter the transient detection unit, and making the steady-state path enter the steady-state detection unit, so that the system may perform transient detection and steady-state detection simultaneously.
The transient detection unit 4 comprises a first focusing lens 41, a first optical filter 42 and a high-speed response detector 43, which are sequentially arranged on the transient road. Wherein the first focusing lens 41 is used for focusing the fluorescence signal of the transient path; the first filter 42 is a long-wave pass filter, which can pass the fluorescent signal and further filter out stray light; the high-speed response detector 43 is a photodetector with a response time in the sub-nanosecond range, which converts a fluorescent signal varying with time into an analog electric signal. Preferably, in this embodiment, the high-speed response detector 43 is a broadband avalanche diode, and the response time can reach 1ns or less, so that the lifetime of the fluorescent signal can be measured better. In other embodiments, the lifetime of the fluorescent signal may be measured using a broadband photodiode with a sufficiently short response time.
The steady state detection unit 5 comprises a multi-channel band-pass filter module 51, a second focusing lens 52, a second optical filter 53 and a high-sensitivity detector 54 which are sequentially arranged on the steady state path. Wherein the multi-channel band-pass filter module 51 is used for respectively passing fluorescent signals with a plurality of specific wavelengths, and at least fluorescent signals with wavelengths of 400nm, 420nm, 430nm and 465nm should be included. Specifically, the multi-channel band-pass filter module 51 in this embodiment is a roller band-pass filter group, which includes a roller frame and 10nm band-pass filters with center wavelengths of 400nm, 420nm, 430nm and 465nm, where each filter respectively forms a wavelength detection channel, and the filtering channels can be switched by rotating the roller frame. The second focusing lens 52 is used to focus the fluorescent signal passing through the multi-channel band-pass filter module 51. The second filter 53 is a long-pass filter, which can pass the fluorescent signal and further filter out stray light. The high sensitivity detector 54 is a photodetector that can sense and convert low intensity fluorescent signals to electrical signals, and should have a sensitivity of at least 1200A/Lm. Specifically, the high sensitivity detector 54 in this embodiment is preferably a photomultiplier tube with an anode sensitivity typically of 1500A/Lm and a peak response wavelength of 400nm, and can effectively measure the intensity of the fluorescent signal passing through each filter channel and convert it to an electrical signal. In other embodiments, the intensity of the fluorescent signal may be measured using an optoelectronic device such as a highly sensitive avalanche diode.
The data acquisition processing unit 7 comprises a data acquisition unit, a fluorescence lifetime fitting module and a light intensity value processing module. The bandwidth of the data collector should be at least 1GHz, the sampling rate is at least 4GS/s, and the storage depth is at least 1Mpts, which includes a transient acquisition module and a steady-state acquisition module, which respectively collect and convert the analog electric signals output by the high-speed response detector 43 and the high-sensitivity detector 54 into digital signals. Wherein the transient acquisition module is triggered by the rising edge of the analog signal output by the high-speed response detector 43 and converts it into a digital signal that varies with time. The steady state acquisition module integrates the electrical signal output by the high sensitivity detector 54, wherein the electrical signal integration time for fluorescence at each wavelength should be greater than 100ms.
And the fluorescence lifetime fitting module carries out convolution fitting analysis on the digital signals obtained in the transient acquisition module. Specifically, the convolution fitting algorithm adopted in this embodiment is as follows:
wherein, t and t' are both time, G (t) is a response function of the system, w is response characteristic time, and the time width of the response function is described and is determined by the excitation light pulse width, the detector bandwidth and the data acquisition device bandwidth; f (t) is a function describing fluorescence excitation and decay, where τ r For the fluorescence rising edge characteristic time, A 0 And t 0 Is the amount of coordinate translation of fluorescence intensity and time. k is the number of actual fluorescence decay processes, which is generally equal to 2 or 3 for autofluorescence of breast tissue and breast tumor, and is determined by the composition of the fluorophore substance of the detection object, A i And τ i The amplitude and lifetime of the corresponding fluorescence decay process, respectively. And f (t') is used for fitting, so that the influence of response broadening of the system can be removed, and the accurate autofluorescence lifetime can be obtained.
Finally, the average fluorescence lifetime τ is calculated from the following formula:
the average fluorescence lifetime tau can be used as a fluorescence lifetime parameter for comparing and discriminating normal tissues and tumor tissues, and the fluorescence lifetime refers to the average fluorescence lifetime tau.
And the light intensity value processing module is used for calculating the digital signals obtained by the steady-state acquisition module so as to realize the discrimination of the tissue to be detected. In the present embodiment, the intensity of the fluorescence signal passing through the four filter channels is set to I in the order of the wavelength from low to high 1 、I 2 、I 3 、I 4 And pass through the formula r= (I 2 +I 3 )/(I 1 +I 4 ) And calculating an R value. Selecting 0.95 as a threshold line, and judging that the tissue to be detected is normal breast tissue when the R value is obviously larger than 0.95; when the R value is obviously smaller than 0.95, the tissue to be detected can be judged to be breast tumor tissue; for the tissue with R value close to 0.95, the comparison result of the fluorescence lifetime and the typical fluorescence lifetime of normal breast tissue is combined to make a judgment.
The following is an example of the detection of breast tumor tissue and normal breast tissue by the breast tumor detection system.
Please refer to fig. 3, which shows the results of fluorescence lifetime detection and convolution fitting for the breast tumor tissue and normal breast tissue, respectively, using the system described in this embodiment. The k value of the convolution fitting algorithm is 2, the autofluorescence lifetime generated by the obtained breast tumor tissue is 2.66ns, the autofluorescence lifetime generated by the normal breast tissue is 4.05ns, and obvious differences exist between the two, so that the detection system has enough time resolution and sensitivity, and the requirements of fluorescence lifetime measurement of the breast tissue and the breast cancer tissue can be met.
Referring to FIG. 4, the results of the light intensity detection of the autofluorescence generated by the system on the breast tumor tissue and normal breast tissue at the wavelengths of 400nm, 420nm, 430nm and 465nm are shown. The light intensity of the two tissues on the selected characteristic wavelength is larger in and out, so that the breast tumor tissue and the normal tissue can be distinguished according to the light intensity difference of the fluorescent signal generated by the tissue to be detected on the four wavelengths.
Please refer to fig. 5, which is a result of collecting fluorescence intensities of the four characteristic wavelengths and further calculating the fluorescence intensities of 16 tissue samples (including 8 breast tumor tissue samples and 8 normal breast tissue samples) using the system. Wherein, the R values of all tumor tissues are smaller than 0.95, and the R values of all normal breast tissues are larger than 0.95, so that 0.95 can be selected as a threshold line for judging the breast tumor tissues and the normal breast tissues.
The invention determines the wavelength of excitation light suitable for discriminating between breast tumor tissue and normal breast tissue, which can make the service life and intensity of fluorescent signals of the two have larger difference so as to be easier to detect. In addition, the invention adopts the hundred picosecond excitation light source, the broadband avalanche photodiode with response time in sub-nanosecond level and the data collector with high bandwidth, high sampling rate and high storage depth to cooperate to carry out fluorescence lifetime detection, and eliminates errors in the system by using a convolution fitting method, thereby realizing high-precision fluorescence lifetime detection effect by using the cost far lower than that of the prior art scheme, and being more beneficial to popularization and application in clinical detection. Furthermore, the spectroscope is utilized to enable the fluorescent signals to enter the transient detection unit and the steady detection unit simultaneously, so that synchronous detection of the fluorescent service life and the fluorescent light intensity can be realized, and compared with a method for detecting the fluorescent service life and the fluorescent light intensity sequentially in the prior art, the method has the advantage that the detection time is saved. Furthermore, compared with the method for detecting the intensity of the fluorescent signal by using equipment such as a grating spectrometer in the prior art, the system disclosed by the invention selects wavelengths of 400nm, 420nm, 430nm and 465nm as characteristic wavelengths for detecting the fluorescent intensity, and uses the R value calculated by the respective light intensity values as a reference standard for distinguishing the breast tumor tissue from the normal breast tissue, so that the cost is lower, the implementation is easier, and the result is more suitable for being used as a reference for distinguishing the breast tumor tissue. In addition, the technical scheme of the invention is also suitable for detecting and screening the cancerous metastasis of the axillary lymph node tissue.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention.
Claims (9)
1. A breast tumor detection system based on autofluorescence, characterized in that: the device comprises an excitation light source, a light conduction unit, a transient detection unit and a data acquisition and processing unit; wherein the excitation light source generates ultraviolet excitation light with a pulse width of hundred picoseconds and a repetition frequency of kilohertz; the light conduction unit transmits the excitation light to irradiate the tissue to be detected, and an autofluorescence signal generated by excitation of the tissue to be detected is transmitted to the transient detection unit through the light conduction unit; the transient detection unit comprises a high-speed response detector with response time at sub-nanosecond level, and the high-speed response detector converts a fluorescent signal returned by the light conduction unit into an electric signal; the data acquisition processing unit comprises a data acquisition unit and a fluorescence lifetime fitting module, wherein the bandwidth of the data acquisition unit is at least 1GHz, the sampling rate is at least 4GS/s, the storage depth is at least 1Mpts, and the data acquisition processing unit converts the electric signals output by the high-speed response detector into digital signals; the fluorescence fitting module carries out convolution fitting processing on the digital signals to obtain the fluorescence life of the self fluorescence signals;
wherein the convolution fit process is represented by:
wherein the method comprises the steps of
Wherein, t and t' are both time, B is the normalization factor of Gaussian response function, w is the response characteristic time of the system, τ r For the fluorescence rising edge characteristic time, A 0 And t 0 The coordinate translation amounts of fluorescence intensity and time respectively, k is the number of fluorescence attenuation processes, A i And τ i The amplitude and the service life of the corresponding fluorescence attenuation process are respectively;
the average fluorescence lifetime τ is calculated from the following formula:
2. the detection system according to claim 1, wherein: the excitation light source comprises a laser and a collimating mirror, wherein the laser is used for generating laser, and the collimating mirror is arranged on a light path of the laser.
3. The detection system according to claim 2, wherein the light conduction unit includes a dichroic mirror having a high reflectance for excitation light and a high transmittance for autofluorescence, a first fiber-optic coupling mirror, an optical fiber, and a second fiber-optic coupling mirror, which are disposed on an optical path of the excitation light and reflect the excitation light into the first fiber-optic coupling mirror, and both ends of the optical fiber are connected to the first fiber-optic coupling mirror and the second fiber-optic coupling mirror, respectively.
4. The detection system of claim 3, wherein the collimating mirror, the first fiber coupling mirror, and the second fiber coupling mirror are quartz lenses, and the optical fiber is an ultraviolet fiber.
5. The detection system according to any one of claims 1 to 4, further comprising a spectroscope and a steady state detection unit, wherein the spectroscope is disposed on an optical path of an autofluorescence signal outputted from the light conduction unit, and divides the fluorescence signal into a transient state path and a steady state path, and makes the fluorescence signal of the transient state path enter the transient state detection unit, and makes the fluorescence signal of the steady state path enter the steady state detection unit; the steady state detection unit comprises a fluorescent signal multichannel band-pass filter device and a high-sensitivity detector which are sequentially arranged on a steady state road, wherein the multichannel band-pass filter device comprises at least two filter channels, and each filter channel can enable fluorescent signals in a specific wavelength range to pass through; the high-sensitivity detector respectively converts the light intensity of fluorescent signals passing through different filtering channels into electric signals; and the data acquisition device also integrates and acquires the electric signals output by the high-sensitivity detector to obtain fluorescent signal intensity values in different wavelength ranges.
6. The detection system according to claim 5, wherein the multi-channel band-pass filter device comprises four filter channels having bandwidths of 10nm and center wavelengths of 400nm, 420nm, 430nm and 465nm, respectively.
7. The detection system according to claim 6, wherein the multi-channel band-pass filter device is a roller band-pass filter group comprising a roller frame and 10nm band-pass filters having center wavelengths of 400nm, 420nm, 430nm, 465nm, respectively.
8. The detection system of claim 7, wherein the excitation light wavelength is 355nm.
9. The system of claim 8, wherein the data acquisition processing unit further comprises a light intensity value processing module configured to process the fluorescent signal intensity values obtained by the data acquisition device for different wavelength ranges according to formula (I 2 +I 3 )/(I 1 +I 4 ) Performing operation, wherein I 1 、I 2 、I 3 、I 4 Sequentially passing through filter channels with central wavelengths of 400nm, 420nm, 430nm and 465nmFluorescent signal intensity value.
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