CN217127451U - Magnetic control cell static mechanical stimulation culture device - Google Patents

Abstract

The utility model provides a magnetic control cell static mechanics stimulates culture apparatus is provided with main part, cell culture portion, be used for right cell culture portion produces the magnetic force portion in magnetic field and is used for adjusting magnetic force portion with thereby the distance adjustment portion of adjusting the static power size between the cell culture portion, cell culture portion assemble in the main part, the inside subassembly that meets an emergency of cell culture portion with magnetic force portion magnetic field is connected, distance adjustment portion movable assembly in the main part, magnetic force portion demountable assembly in distance adjustment portion. The utility model discloses utilize the magnetic field can not receive the separation and the characteristics of conduction effort, can also make cell culture portion relatively independent when producing static power to cell culture portion, from reducing the risk of dying fungus, but also can the amazing range of accurate regulation static mechanics.

Description

Magnetic control cell static mechanical stimulation culture device

Technical Field

The utility model belongs to biomechanics instrument field, in particular to a magnetic control cell static mechanics stimulation culture device.

Background

The natural skeletal muscles, bones, cartilages, skins and other tissues and organs are subjected to certain long-time static mechanical load in a physiological state, for example, the bones and the cartilages need to bear the long-time static compression load of body weight, part of the skeletal muscles maintain the normal posture of a human body through static isometric contraction, and the skins in part of the regions are pressed or pulled for a long time in certain body states, such as the skins of the head rest in a supine position. The cells of these tissues and organs are also subjected to static mechanical stress for a long time in a physiological state.

Cell biomechanics is a leading branch of the field of biomechanics, and relates to the study of the change rule and mechanism action of the cell state under the action of mechanical load. The existing reports show that long-time static mechanical stimulation plays an important role in promoting the orientation arrangement of the organ tissue cells, inducing maturation differentiation, expressing related proteins and the like. Therefore, with the progress of life science research in recent years, when researchers construct the in vitro biomimetic model with the tissue and organ function, a method for conveniently and effectively providing biomimetic static mechanical stimulation to the tissue cells is urgently needed.

The existing cell static mechanical load loading equipment is specifically divided into mechanical loading equipment, manual loading equipment and pneumatic loading equipment according to the generation and conduction modes of acting force.

The mechanical loading device is used for directly connecting a force-bearing body (such as a material or a tissue block with cells) with a transmission rod, and then driving the transmission rod through a motor to adjust the static mechanical force with different amplitudes to be loaded on the force-bearing body. The mechanical loading equipment is mainly adopted by current scientific researches, but has the following defects: 1. in the process of back-and-forth drawing of the transmission rod, external bacteria are easily brought into the cell culture bin, so that bacteria contamination is caused. 2. Because of the presence of the actuator rod, the cell culture chamber would have to be integrated with the loading force source and control system, which would not facilitate modular partition management of the various components. For example, the transfer bar has to be removed when replacing the culture chamber; the contamination problem of the culture bin needs to be considered when the motor is overhauled.

The manual loading device is used for directly connecting a force bearing body (such as a material or a tissue block with cells) with the bolt, and then regulating different magnitudes of static mechanical force to be loaded on the force bearing body by manually changing the position of the bolt. The mechanical loading equipment is also adopted by the current scientific research mainstream, but has the following disadvantages: the cell culture chamber needs to be opened, and the position of the static latch is manually adjusted to adjust the amplitude of the static mechanical force. However, the experimental operation is complicated, a sterile environment and sterile operation are required, and the open operation is easy to cause bacterial contamination in the cell culture bin.

The pneumatic loading equipment is characterized in that two ends of the stress body are respectively connected with the air bags, and then the expansion or contraction amplitude of the air bags is changed by ventilating or deflating the air bags, so that the stress body is driven to deform. The instrument has the industrialized commodities at present, such as a commercial Flexcell5000 type, can keep the independence of a cell culture bin to a certain extent and avoid contamination, but is mainly used for researching dynamic mechanical stimulation on cells at present, and the instrument has a complex structure, a high selling price and a small deformation degree.

Therefore, it is necessary to provide a magnetic-controlled cell static mechanical stimulation culture device to solve the deficiencies of the prior art.

SUMMERY OF THE UTILITY MODEL

The utility model aims to avoid the defects of the prior art and provide a magnetic control cell static mechanics stimulation culture device. The magnetic control cell static mechanical stimulation culture device utilizes the characteristic that a magnetic field can conduct acting force without being blocked, generates static force on a cell culture part, enables the cell culture part to be relatively independent, reduces the risk of contamination, and can accurately adjust the amplitude of static mechanical stimulation.

The above object of the present invention is achieved by the following technical measures:

the utility model provides a magnetic control cell static mechanics amazing culture apparatus, be provided with main part, cell culture portion, be used for right cell culture portion produces magnetic field magnetic force portion and is used for adjusting magnetic force portion with thereby the distance adjustment portion of adjusting the static power size between the cell culture portion, cell culture portion assemble in the main part, the inside strain assembly of cell culture portion with magnetic force portion magnetic field is connected, distance adjustment portion movable assembly in the main part, magnetic force portion demountable assembly in distance adjustment portion.

Preferably, the distance adjusting part is provided with a containing bin for containing the magnetic part and an adjusting component, the magnetic part is detachably assembled on the containing bin, the containing bin is fixedly assembled on the adjusting component, and the adjusting component is assembled on the main body.

Preferably, above-mentioned adjusting part is provided with drive screw, nut piece, driving medium and backup pad, backup pad fixed assembly in the main part, the one end of drive screw run through in backup pad one end and with driving medium fixed connection, the other end of drive screw assemble in the other end of backup pad, the nut piece assemble in the surface of drive screw, just the internal surface of nut piece with drive screw threaded connection, hold the storehouse with nut piece fixed assembly, the nut piece with backup pad sliding connection.

Preferably, the adjusting assembly is further provided with a guide rod, two ends of the guide rod are respectively assembled on the supporting plate, and the guide rod penetrates through the nut block.

Preferably, the transmission member is a turntable.

Preferably, the cell culture part is provided with a culture bin and a magnetizer for conducting the magnetic field of the magnetic force part to the strain assembly, the culture bin is assembled on the main body, the strain assembly is detachably assembled inside the culture bin, the distance adjusting part is positioned outside the culture bin, the magnetizer is embedded on the bottom surface of the culture bin and corresponds to the position of the strain assembly, and the magnetizer is connected with the strain assembly and the magnetic force part through the magnetic field.

Preferably, the cultivation bin is provided with a storage tank and an assembly tank, the storage tank is located at the bottom of the cultivation bin, the storage tank is communicated with the main space of the cultivation bin, and the strain assembly is arranged in the storage tank.

Preferably, the assembling slot is located at the bottom of the culture bin and is not communicated with the main space of the culture bin, the opening of the assembling slot faces the direction of the accommodating bin, and the magnetizer is embedded in the assembling slot.

Preferably, the opening of the fitting groove is in movable contact with the magnetic portion.

Preferably, the central axis of the magnetizer overlaps the central axis of the magnetic portion.

Preferably, the opening of the accommodating chamber faces the direction of the assembling groove.

Preferably, the strain assembly is provided with a stator, a magnetic absorption rotor and a force bearing body for bearing cells, one side of the force bearing body is fixedly connected with the stator, the other side of the force bearing body is fixedly connected with the magnetic absorption rotor, the stator and the magnetic absorption rotor are arranged oppositely, the stator is fixedly assembled inside the culture bin, and the magnetic absorption rotor moves inside the culture bin.

Preferably, the storage tank is divided into a fixed groove, a stretching groove and a liquid changing groove, the stretching groove and the liquid changing groove are respectively communicated with the fixed groove, the stator is detachably embedded in the fixed groove, the magnetic absorption rotor is movably arranged in the stretching groove, and the stretching groove is adjacent to the assembly groove.

Preferably, the magnetic attracting element and the magnetic part are all neodymium iron boron magnets.

Preferably, the stator is a silicon stator.

Preferably, the stress body is an elastic membrane, a 3D printed bionic tissue or a tissue mass.

Preferably, the main body is provided with an observation window for observation by an external microscope, the observation window being located at a bottom of the main body and corresponding to the strain module, and a level gauge mounted on a bottom plate of the main body and located on the cell culture part side.

The utility model discloses a magnetic control cell static mechanics amazing culture apparatus is provided with main part, cell culture portion, is used for right cell culture portion produces the magnetic force portion in magnetic field and is used for adjusting magnetic force portion with thereby the distance adjustment portion of static power size is adjusted to the distance between the cell culture portion, cell culture portion assemble in the main part, the inside subassembly that meets an emergency of cell culture portion with magnetic force portion magnetic field is connected, apart from adjustment portion movable assembly in the main part, magnetic force portion demountable assembly in apart from adjustment portion. Compared with the prior art, the utility model beneficial effect be: (1) the mode of applying biomechanical load by using a magnetic field is adopted, and the distribution of the magnetic field is not blocked by the cell culture part, so that the magnetic part and the cell culture part can be completely separated, the contamination probability of the cell culture part is reduced, and the whole modular partition management and work are facilitated. (2) The utility model discloses an apply the magnetic field of biomechanics load and produce by magnetic force portion, consequently can be through the distance of control magnetic force portion and the orientation of the utmost point S of N, can control the power and the polarity direction in magnetic field to the control of mechanics is more convenient effective. (3) The utility model discloses an overall arrangement is simple to reduce the production degree of difficulty.

Drawings

The present invention will be further described with reference to the accompanying drawings, but the contents in the drawings do not limit the present invention in any way.

FIG. 1 is a schematic structural diagram of a magnetic control cell static mechanical stimulation culture device.

Fig. 2 is another angle view of fig. 1.

Fig. 3 is a schematic sectional view taken along the line "a-a" in fig. 2.

Fig. 4 is a schematic structural view of the distance adjusting part.

FIG. 5 is a schematic view of the structure of the culture chamber.

FIG. 6 is a schematic view of the structure of the strain assembly and the magnetizer after being assembled in the cultivation container.

FIG. 7 is a schematic diagram showing the structure of a cell culture part.

FIG. 8 is a schematic view of the positioning of the embedding instrument.

Fig. 9 is a schematic structural view of the strain gauge assembly when the positioning insert gauge is assembled to the strain gauge assembly.

Fig. 10 is an exploded view of fig. 9.

FIG. 11 is a graph showing the stretching effect of the force-receiving body at different distances from the magnetic force unit and the cell culture unit.

Fig. 12 is a graph of the strain versus distance of the corresponding force-bearing body of fig. 11.

Fig. 13 is a schematic view of the magnetic force portion approaching the strain assembly.

FIG. 14 is a schematic diagram showing the cell change after stretching in FIG. 13.

FIG. 15 is a schematic view of the magnetic portion approaching the strain assembly.

FIG. 16 is a schematic diagram showing the cell change after compression in FIG. 15.

FIG. 17 shows the orientation and differentiation effects of unstretched C2C12 mouse myoblasts after 5 days of culture.

Fig. 18 is an orientation distribution diagram of fig. 17.

FIG. 19 shows the orientation and differentiation effects of the stretched C2C12 mouse myoblasts after 5 days of culture.

Fig. 20 is an orientation distribution diagram of fig. 19.

In fig. 1 to 20, there are included:

a main body 100, an observation window 110, a level 120,

A cell culture part 200,

A culture bin 210, a fixing groove 2111, a stretching groove 2112, a liquid changing groove 2113, an assembling groove 2114, a buoyancy component 212, a buoyancy dish 2121, a positioning iron block 2122, a,

A strain assembly 220, a stator 221, a magnetic mover 222, a stressed body 223,

A magnetizer 230,

A magnetic part 300,

Distance adjusting part 400, accommodating bin 410, adjusting component 420, transmission screw 421, nut block 422, transmission piece 423, supporting plate 424, guide rod 425,

A silica gel mold 500,

The positioning embedding instrument 600, the frame body 610, the clamp 620, the Z-axis lifting adjusting part 630 and the XY-axis translation adjusting part 640.

Detailed Description

The technical solution of the present invention will be further explained by the following examples.

Example 1.

A magnetic-controlled cell static-mechanical-stimulation culture apparatus, as shown in FIGS. 1 to 10, comprises a main body 100, a cell culture part 200, a magnetic force part 300 for generating a magnetic field to the cell culture part 200, and a distance adjustment part 400 for adjusting the distance between the magnetic force part 300 and the cell culture part 200 to adjust the magnitude of static force, wherein the cell culture part 200 is assembled to the main body 100, a strain assembly 220 inside the cell culture part 200 is magnetically connected to the magnetic force part 300, the distance adjustment part 400 is movably assembled to the main body 100, and the magnetic force part 300 is detachably assembled to the distance adjustment part 400.

It should be noted that, for the strain element 220 to be stretched or pressed, it is only necessary to switch the north pole and the south pole of the magnetic part 300, and as shown in fig. 13 to 16, the magnitude of the acting force can be adjusted by changing the distance between the magnetic part 300 and the cell culture part 200, wherein the smaller the distance between the two parts, the larger the acting force, and the larger the distance, the smaller the acting force. The static force of the present invention is a magnetic force generated by a magnetic field.

The distance adjusting part 400 is provided with a housing 410 for housing the magnetic force part 300 and an adjusting assembly 420, the magnetic force part 300 is detachably assembled to the housing 410, the housing 410 is fixedly assembled to the adjusting assembly 420, and the adjusting assembly 420 is assembled to the main body 100.

The adjusting assembly 420 is provided with a transmission screw 421, a nut block 422, a transmission member 423 and a support plate 424, the support plate 424 is fixedly assembled on the main body 100, one end of the transmission screw 421 penetrates through one end of the support plate 424 and is fixedly connected with the transmission member 423, the other end of the transmission screw 421 is assembled at the other end of the support plate 424, the nut block 422 is assembled on the outer surface of the transmission screw 421, the inner surface of the nut block 422 is in threaded connection with the transmission screw 421, the accommodating chamber 410 is fixedly assembled with the nut block 422, and the nut block 422 is slidably connected with the support plate 424.

The adjusting assembly 420 is further provided with a guide rod 425, both ends of the guide rod 425 are respectively assembled to the supporting plates 424, and the guide rod 425 penetrates the nut block 422. The transmission member 423 of the embodiment is a rotary disc, and an operator can rotate the rotary disc manually to further drive the transmission screw 421 to rotate, so that the nut 422 moves forwards or backwards, and the distance between the magnetic part 300 and the cell culture part 200 can be adjusted.

It should be noted that the transmission member 423 of the present invention may be a motor, and when the motor is used, the adjustment of the distance between the magnetic portion 300 and the cell culture portion 200 can be achieved by controlling the motor.

The utility model discloses a cell culture portion 200 is provided with cultivates storehouse 210 and conducts the magnetizer 230 to meeting an emergency subassembly 220 with the magnetic field of magnetic force portion 300, cultivates storehouse 210 and assembles in main part 100, meets an emergency subassembly 220 demountable assembly in the inside of cultivateing storehouse 210, apart from regulating part 400 to be located the outside of cultivateing storehouse 210, magnetizer 230 inlays the dress in the bottom surface of cultivateing storehouse 210 and corresponding with the position of meeting an emergency subassembly 220, and magnetizer 230 is connected with meeting an emergency subassembly 220 and magnetic force portion 300 magnetic field.

The cultivation bin 210 is provided with a storage groove and an assembly groove 2114, the storage groove is positioned at the bottom of the cultivation bin 210 and is in spatial communication with the main body 100 of the cultivation bin 210, and the strain assembly 220 is arranged in the storage groove.

The assembly slot 2114 is located at the bottom of the culture chamber 210 and is not spatially communicated with the main body 100 of the culture chamber 210, the opening of the assembly slot 2114 faces the direction of the accommodating chamber 410, and the magnetizer 230 is embedded in the assembly slot 2114.

The strain assembly 220 is provided with a stator 221, a magnetic mover 222 and a force-bearing body 223 for bearing cells, one side of the force-bearing body 223 is fixedly connected with the stator 221, the other side of the force-bearing body 223 is fixedly connected with the magnetic mover 222, the stator 221 and the magnetic mover 222 are arranged oppositely, the stator 221 is fixedly assembled inside the culture bin 210, and the magnetic mover 222 is movable inside the culture bin 210.

It should be noted that, in the pneumatic loading device of the prior art, for the large-volume force-bearing body, the corresponding large-volume air bag needs to be used for expansion or contraction. The utility model discloses a subassembly 220 that meets an emergency only needs can stretch or compress in the relative both sides limit of atress body 223, consequently the utility model discloses a magnetism inhale active cell 222 and stator 221 only need be fixed in two relative sides of atress body 223 can. That is, both sides of the force-receiving body 223 only need to be capable of being fixed to the stator 221 and the magnetic mover 222, and for force-receiving bodies 223 with different lengths and volumes, the same stator 221 and magnetic mover 222 may be used for static force stimulation, so that the stator 221 and the stator 221 are not limited by the volume of the force-receiving body 223.

Will the utility model discloses a atress body 223 is the atress cross section along the cross section definition of tensile or compression direction, when magnetic force portion 300 produced magnetic field to magnetism suction rotor 222, and the effort of magnetism suction rotor 222 conducts to another side of atress body 223 from a side of atress body 223 along this atress cross section. In practical cases, the stressed body is generally in a strip shape or a rectangular parallelepiped shape, and the area of the stressed cross section is much smaller than that of the stressed body 223, so that the pressure of the acting force on the stressed cross section is larger, and larger strain can be realized.

The utility model discloses a stress 223 can be the bionic tissue or the tissue piece etc. that elastic membrane, 3D printed, and wherein the elastic membrane specifically is PDMS silica gel membrane, when being the elastic membrane, can directly inoculate the cell in the elastic membrane. The culture chamber 210 may be made of non-cytotoxic materials such as glass, acrylic, photosensitive resin, or transparent silica gel.

The storage groove is divided into a fixing groove 2111, a stretching groove 2112 and a liquid changing groove 2113, the stretching groove 2112 and the liquid changing groove 2113 are respectively communicated with the fixing groove 2111, the stator 221 is detachably embedded in the fixing groove 2111, the magnetic attracting rotor 222 moves in the stretching groove 2112, and the stretching groove 2112 is adjacent to the assembling groove 2114. The liquid replacement groove 2113 of the present invention is for enabling replacement of the culture liquid in the stretching groove 2112 without contacting the stretching groove 2112 in which the force receiving member is located.

The opening of the assembling groove 2114 of the utility model is movably abutted against the magnetic force part 300; the central axis of the magnetizer 230 is overlapped with the central axis of the magnetic part 300; the opening of the accommodating chamber 410 faces in the direction in which the fitting groove 2114 is located.

Note that the movable contact of the opening of the fitting groove 2114 with the magnetic force portion 300 according to the present invention means that the magnetic force portion 300 can be moved to the opening of the fitting groove 2114 by adjusting the distance by the distance adjusting portion 400, and in this state, the maximum strain acting force can be generated in the strain element 220. The central axis of the magnetic conductor 230 overlaps the central axis of the magnetic part 300, so that the force receiver 223 is kept stretched or squeezed along a straight line during the application of force by the magnetic part 300.

The opening of the accommodating chamber 410 faces the assembling slot 2114, so that the magnetic force portion 300 is directly opposite to the magnetic conductor 230, which avoids the obstruction between the magnetic force portion 300 and the magnetic conductor 230 by the wall surface of the accommodating chamber 410 and the like, and reduces the static force transmission effect. Under the action of the distance adjusting part, the magnetic part 300 and the magnetizer 230 can be directly abutted against each other, so that the distance between the magnetic part and the magnetizer is shortened to the maximum extent, and the strength of the static force is improved.

The utility model discloses a magnetic suction active cell 222 and magnetic force portion 300 are neodymium iron boron magnet, and stator 221 is silica gel stator 221, and atress body 223 is the bionic tissue or the tissue piece that elastic membrane, 3D printed. The magnetizer 230 is a magnetically permeable iron core. The utility model discloses a magnetic force portion 300 is cylindric neodymium iron boron magnet, and neodymium iron boron magnet's raw materials are the neodymium iron boron powder of N52, N48 or N44 model, and wherein cylindric diameter range is 4cm ~ 10cm, and the high range is 4 ~ 10 cm.

The main body 100 of the present invention is provided with an observation window 110 and a level gauge 120 for observing by an external microscope, the observation window 110 is located at the bottom of the main body 100 and corresponds to the position of the strain assembly 220, and the level gauge 120 is assembled on the bottom plate of the main body 100 and located on one side of the cell culture part 200.

The observation window 110 of the present invention is used to allow the cells of the stressed body 223 to be directly observed inside the culture chamber 210 by installing a microscope below the main body 100 and directly observing the cells inside the culture chamber 210 through the observation window 110. The observation window 110 of the present invention corresponds to the position of the strain assembly 220, that is, enables the microscope to observe the position of the stress body 223 of the strain assembly 220, specifically under the stress body 223. The utility model discloses a spirit level 120's effect is that the instruction cultivates the horizontal position of storehouse 210, and then makes and cultivate storehouse 210 and be in the level, because the microscope will focus, if cultivate storehouse 210 (or atress body 223) and not be in the horizontality when observing, need focus constantly when so observing different places, can cause the operation inconvenience like this.

The stator 221 of the present invention is a hollow stator 221, and one side of the force-bearing body 223 is fixedly embedded in the hollow stator 221; the magnetic mover 222 is a hollow magnetic mover 222, and the other side of the force receiver 223 is fixedly inserted into the hollow magnetic mover 222. The utility model discloses a magnetic attraction active cell 222's surface parcel has first elastic layer, a side and the first elastic layer body coupling of atress body 223. The outer surface of the stator 221 is wrapped with a second elastic layer, and the second elastic layer is integrally connected to the other side of the force-bearing body 223.

It should be noted that the stator 221 and the magnetic attraction rotor 222 of the present invention are both hollow structures, and both sides of the force bearing body 223 are both fixed and embedded in the two hollow structures. The purpose of this is to increase the contact area between the stator 221 and the magnetic mover 222 and the force-receiving body 223, thereby improving the firmness of the force-receiving body 223 and the stator 221 and the magnetic mover 222 in tension or compression. The two side edges of the stressed body 223 of the utility model are fixed with the magnetic attraction rotor 222 and the stator 221 through the first elastic layer and the second elastic layer respectively. The utility model discloses a first elastic layer and second elastic layer are PDMS silica gel layer.

The cell culture part 200 further includes a buoyancy module 212 for suspending the magnetic mover 222 in the culture chamber 210, and a cover body, wherein the buoyancy module 212 floats on the surface of the culture medium stored in the culture chamber 210, the strain module 220 is detachably mounted in the culture chamber 210, and the cover body is fastened to the upper bottom surface of the culture chamber 210.

The utility model discloses a buoyancy subassembly 212 is provided with buoyancy ware 2121 and location iron plate 2122, and buoyancy ware 2121 floats in the inside of cultivateing the storehouse 210, and the center in buoyancy ware 2121 is placed to location iron plate 2122, and the connection is inhaled with magnetism to location iron plate 2122 and inhale active cell 222 magnetism.

Wherein, the cover body of the utility model is a commercially available 100 multiplied by 100mm square polystyrene cell culture dish cover.

It should be noted that, after the culture chamber 210 contains the culture fluid, the buoyancy plate 2121 can float inside the culture chamber 210, at this time, the positioning iron block 2122 is magnetically connected to the magnetic mover 222, and the buoyancy plate 2121 simultaneously generates buoyancy to the positioning iron block 2122 and the magnetic mover 222. Therefore, the magnetic mover 222 can be suspended in the culture medium, and the magnetic mover 222 does not contact the bottom surface of the culture chamber 210, thereby reducing the friction between the magnetic mover 222 and the culture chamber 210 and ensuring the smooth stretching or compressing and elastic restoration under the action of the magnetic field.

The sum of the lengths of the stretching grooves 2112 and the fixing grooves 2111 is defined as A, the length of the strain element 220 in the original state is defined as B, and 1cm < 1.5B < A < 3B is present.

When the length of the stretching slot 2112 is 1.5 times or more the length of the strain assembly 220 in the original state, a sufficient moving distance can be provided for the magnetically attracting mover 222.

The utility model discloses a magnetic control cell static mechanics stimulation culture apparatus still is provided with and is used for inlaying atress body 223 in stator 221 and magnetism inhale active cell 222's location embedding appearance 600. The positioning insertion instrument 600 is provided with a frame body 610, a fixture 620 for fixing the force-bearing body 223, the stator 221 or the magnetic mover 222, a Z-axis elevation adjusting part 630 and an XY-axis translation adjusting part 640, wherein the fixture 620 is assembled on the frame body 610, and the XY-axis translation adjusting part 640 is fixedly assembled on the upper bottom surface of the Z-axis elevation adjusting part 630, as shown in fig. 8.

Note that, the Z-axis elevation adjusting portion 630, the XY-axis translation adjusting portion 640, and the jig 620 of the present invention are all commercially available.

The use method of the magnetic control cell static mechanical stimulation culture device of the utility model is as follows: the strain assembly 220 with cells is assembled in the culture chamber 210, and the distance between the accommodating chamber 410 and the whole magnetic force part 300 to the cell culture part 200 is adjusted by the rotary transmission member 423 according to actual requirements, so that the magnetic force generated by the magnetic force part 300 is applied to the magnetic attracting mover 222, and finally, the stress body 223 is stretched or extruded to generate acting force, and the purpose of static mechanical stimulation is realized.

Wherein, the utility model discloses a particular fixed method of atress body 223 and magnetism active cell 222 and stator 221 is as follows:

firstly, coating and wrapping the surface of the stress body 223 by using protein, clamping one side edge of the stress body 223 on a clamp 620, and allowing the other end of the stress body 223 to freely droop under the action of gravity;

secondly, placing the magnetic mover 222 into a prefabricated PDMS silica gel mold 500, placing the magnetic mover 222 and the PDMS silica gel mold in an XY-axis translation adjusting part 640, and adjusting the XY-axis translation adjusting part 640 and the Z-axis lifting adjusting part 630 so that the stressed body 223 is just positioned in the center of the hollow structure of the magnetic mover 222;

thirdly, pouring uncured PDMS silica gel into the prefabricated PDMS silica gel mold 500, so that one side of the stressed body 223 and the magnetic attraction mover 222 are completely immersed in the PDMS silica gel;

fourthly, the fixture 620, the stressed body 223, the magnetic rotor 222 and the PDMS silica gel mold 500 are integrally placed in a 60-degree oven for one hour to complete the curing of the PDMS silica gel, so that one side of the magnetic rotor 222 and one side of the stressed body 223 are completely embedded in the prefabricated PDMS silica gel mold 500, and the cured PDMS silica gel is a first elastic layer;

fifthly, clamping the magnetic rotor 222 with the elastic layer wrapped on the surface in a clamp 620, and allowing the other end of the stress body 223 to freely droop under the action of gravity;

sixthly, the stator 221 is placed in the prefabricated PDMS silica gel mold 500, and then the stator 221 and the PDMS silica gel mold are placed in the XY-axis translation adjusting part 640, and the XY-axis translation adjusting part 640 and the Z-axis lifting adjusting part 630 are adjusted, so that the other side edge of the stress body 223 is just positioned in the center of the hollow structure of the stator 221;

seventhly, pouring uncured PDMS silica gel into the prefabricated PDMS silica gel mold 500, so that the other side of the stress body 223 and the stator 221 are completely immersed in the PDMS silica gel, as shown in FIGS. 9 and 10;

eighthly, the fixture 620, the stressed body 223, the magnetic attracting rotor 222, the stator 221 and the PDMS silica gel mold 600 are integrally placed in a 60-degree oven for one hour to complete the curing of the PDMS silica gel, as shown in fig. 9 and 10, the other side edges of the stator 221 and the stressed body 223 are completely embedded in the prefabricated PDMS silica gel mold 700, the cured PDMS silica gel is a second elastic layer, and the strain assembly 220 is obtained.

The magnetic control cell static mechanical stimulation culture device has the beneficial effects that: (1) by applying the biomechanical load by the magnetic field, the distribution of the magnetic field is not blocked by the cell culture part 200, so the magnetic part 300 and the cell culture part 200 can be completely separated, the contamination probability of the cell culture part 200 is reduced, and the whole modularized partition management and work are facilitated. (2) The utility model discloses an exert the magnetic field of biomechanics load and produce by magnetic force portion 300, consequently can be through the distance of control magnetic force portion 300 and the orientation of the utmost point S of N, can control the power and the polarity direction in magnetic field to the control of mechanics is more convenient effective. (3) The utility model discloses an overall arrangement is simple to reduce the production degree of difficulty.

Example 2.

The other characteristics of the magnetic control cell static mechanical stimulation culture device are the same as the embodiment 1, and the difference is that: the force-bearing body 223 of this embodiment is an elastic membrane, and the elastic membrane is specifically a PDMS silica gel membrane.

The magnetic part 300 of this embodiment is a cylindrical neodymium iron boron magnet with a diameter of 4cm and a height of 4cm, and the raw material of the neodymium iron boron magnet is neodymium iron boron powder of N52.

As shown in FIGS. 11 and 12, the amount of tensile strain of the elastic membrane is measured when the distance between the magnetic force part 300 and the cell culture part 200 is 1.5cm, 2.0cm, 2.5cm, 3.0cm, 3.5cm, 4.0cm, 4.5cm, and 5.0 cm.

In the present example, the amount of deformation of the elastic membrane is increased as the distance between the magnetic force part 300 and the cell culture part 200 is decreased, and the amount of tensile strain is more than 15% when the distance is 1.5 cm.

Example 3.

The utility model provides a magnetic control cell static mechanics amazing culture apparatus, the utility model discloses magnetic control cell static mechanics amazing culture apparatus's application method as follows: cutting a PDMS (polydimethylsiloxane) silicon film with the thickness of 0.08mm into a required shape, and fixing the stress body 223, the magnetic absorption rotor 222 and the stator 221 to obtain the strain assembly 220. And soaking in 75% ethanol for 30 min to sterilize the strain assembly 220, and air drying in a clean bench. The cultivation bin 210 is sterilized by soaking in 75% ethanol for 30 minutes, and after the cultivation bin 210 is air-dried in a clean bench, the stator 221 of the strain assembly 220 is installed in the fixing groove 2111 of the cultivation bin 210.

Then, the cells were seeded on the surface of the stressed body 223, and the myoblasts of the C2C12 mouse within 10 passages of the pancreatin were used as specific cells in this example. Finally, the magnetic force part 300 generates a magnetic field to the magnetic mover 222, so that the magnetic mover 222 statically stretches the stressed body 223, and the orientation distribution of the cytoskeleton of the myoblasts of the C2C12 mouse after 5 days of culture is shown in FIG. 19.

Mouse myoblasts not statically stretched under otherwise identical conditions, as shown in fig. 17, where it can be seen that cytoskeletal orientation is quantitatively analyzed in a "swirl-like" distribution (generally adherence is the normal distribution morphology of cells), and cell orientation appears approximately normal in the 0-90 degree direction, as shown in fig. 18. And through the utility model discloses a static tensile mouse myoblast of magnetic control cell static mechanics stimulation culture apparatus, like figure 19, can see from the picture that cytoskeleton orientation is concentrated on 90 degrees directions more, sees with tensile direction is perpendicular, through the utility model discloses a static tensile cell orientation is concentrated on with the vertical direction, like figure 20.

Wherein, the C2C12 mice within 10 generations of the utility model are inoculated with pancreatin. The cell inoculation method is explained in the utility model, which comprises the following steps: centrifuging myoblasts of C2C12 mice in 10 generations of pancreatin digestion, diluting the whole culture to 20-30% or 50-70% of cell inoculation density after whole culture and heavy suspension, and dripping 200 microliters of the cells on a stress body 223 to finish cell inoculation. Then, after standing at 37 ℃ for 3 hours, the cells were preliminarily attached.Continuously adding 50 ml-60 ml of the whole culture solution, installing a buoyancy dish 2121 and a positioning iron block 2122 under the aseptic operation, and covering the culture bin 210. Then the magnetizer 230 is embedded into the assembly groove 2114, and the whole cell culture bin 210 is put into the incubator at 37 ℃ and 5% CO ₂ Standing for 12h under the condition to complete the adhesion of the cells.

After the static mechanical stimulation is performed on the muscle cells by the magnetic control cell static mechanical stimulation culture device, most of the cells are oriented.

Example 4.

A method of static mechanical stimulation of magnetic-controlled cells, using a device for static mechanical stimulation of magnetic-controlled cells as in examples 1 to 3.

The utility model discloses a distance between magnetic force portion 300 and the cell culture portion 200 is adjusted to distance adjustment portion 400, and then adjusts and apply in the static force size of meeting an emergency subassembly 220.

The magnetic control cell static mechanical stimulation method has the beneficial effects that: (1) by applying the biomechanical load by the magnetic field, the distribution of the magnetic field is not blocked by the cell culture part 200, so the magnetic part 300 and the cell culture part 200 can be completely separated, the contamination probability of the cell culture part 200 is reduced, and the whole modularized partition management and work are facilitated. (2) The utility model discloses an exert the magnetic field of biomechanics load and produce by magnetic force portion 300, consequently can be through the distance of control magnetic force portion 300 and the orientation of the utmost point S of N, can control the power and the polarity direction in magnetic field to the control of mechanics is more convenient effective. (3) The utility model discloses an overall arrangement is simple to reduce the production degree of difficulty.

It should be finally noted that the above embodiments are only intended to illustrate the technical solutions of the present invention and not to limit the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced with equivalents without departing from the spirit and scope of the technical solutions of the present invention.

Claims (10)

1. A magnetic control cell static mechanical stimulation culture device is characterized in that: be provided with main part, cell culture portion, be used for right cell culture portion produces magnetic force portion in magnetic field and is used for adjusting magnetic force portion with thereby the distance adjustment portion of adjusting the static power size between the cell culture portion, cell culture portion assemble in the main part, the inside subassembly that meets an emergency of cell culture portion with magnetic force portion magnetic field is connected, distance adjustment portion movable assembly in the main part, magnetic force portion demountable assembly in distance adjustment portion.

2. The device for static mechanical stimulation and culture of magnetic control cells according to claim 1, characterized in that: the distance adjusting part is provided with a containing bin and an adjusting component which are used for containing the magnetic force part, the magnetic force part is detachably assembled in the containing bin, the containing bin is fixedly assembled in the adjusting component, and the adjusting component is assembled in the main body.

3. The device for static mechanical stimulation and culture of magnetic control cells according to claim 2, characterized in that: the adjusting part is provided with transmission lead screw, nut piece, driving medium and backup pad, backup pad fixed assembly in the main part, the one end of transmission lead screw run through in backup pad one end and with driving medium fixed connection, the other end of transmission lead screw assemble in the other end of backup pad, nut piece assemble in the surface of transmission lead screw, just the internal surface of nut piece with transmission lead screw threaded connection, hold the storehouse with nut piece fixed assembly, nut piece with backup pad sliding connection.

4. The device for static mechanical stimulation and culture of magnetic control cells according to claim 3, wherein: the adjusting component is also provided with a guide rod, two ends of the guide rod are respectively assembled on the supporting plate, and the guide rod penetrates through the nut block;

the transmission part is a turntable.

5. The device for the static mechanical stimulation and culture of the magnetic controlled cells according to any one of the claims 2 to 4, which is characterized in that: the cell culture portion is provided with cultivates the storehouse and will the magnetic field of magnetic force portion conducts extremely the magnetizer of subassembly that meets an emergency, cultivate the storehouse assemble in the main part, the subassembly that meets an emergency demountable assembly in cultivate the inside in storehouse, the distance adjusting part is located cultivate the outside in storehouse, the magnetizer inlays the dress in cultivate the bottom surface in storehouse and with the position of subassembly that meets an emergency corresponds, just the magnetizer with the subassembly that meets an emergency with magnetic force portion magnetic field connects.

6. The device for static mechanical stimulation and culture of magnetic control cells according to claim 5, wherein: the culture bin is provided with a storage groove and an assembly groove, the storage groove is positioned at the bottom of the culture bin and is communicated with the main space of the culture bin, and the strain assembly is arranged in the storage groove;

the assembly groove is positioned at the bottom of the culture bin and is not communicated with the main body space of the culture bin, the opening of the assembly groove faces the direction of the containing bin, and the magnetizer is embedded in the assembly groove.

7. The device for static mechanical stimulation and culture of magnetic control cells according to claim 6, wherein: the opening of the assembling groove is movably abutted against the magnetic part;

the central axis of the magnetizer is overlapped with the central axis of the magnetic part;

the opening of the containing bin faces to the direction of the assembling groove.

8. The device for static mechanical stimulation and culture of magnetic control cells according to claim 7, wherein: the strain assembly is provided with a stator, a magnetic absorption rotor and a stress body for bearing cells, one side edge of the stress body is fixedly connected with the stator, the other side edge of the stress body is fixedly connected with the magnetic absorption rotor, the stator and the magnetic absorption rotor are arranged oppositely, the stator is fixedly assembled inside the culture bin, and the magnetic absorption rotor moves inside the culture bin;

the storage tank is divided into a fixed groove, a stretching groove and a liquid changing groove, the stretching groove and the liquid changing groove are respectively communicated with the fixed groove, the stator is detachably embedded in the fixed groove, the magnetic absorption rotor moves in the stretching groove, and the stretching groove is adjacent to the assembly groove.

9. The device for static mechanical stimulation and culture of magnetic control cells according to claim 8, wherein: the magnetic absorption rotor and the magnetic force part are all neodymium iron boron magnets;

the stator is a silica gel stator;

the stress body is an elastic membrane, a bionic tissue printed in a 3D mode or a tissue block.

10. The device for static mechanical stimulation and culture of magnetic control cells according to claim 1, characterized in that: the main part is provided with an observation window and a level meter which are used for observing by an external microscope, the observation window is positioned at the bottom of the main part and corresponds to the strain component, and the level meter is assembled on the bottom plate of the main part and positioned on one side of the cell culture part.

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