CN102965286B - Magnetically-driven sliding load device capable of controlling compression amount - Google Patents
Magnetically-driven sliding load device capable of controlling compression amount Download PDFInfo
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- CN102965286B CN102965286B CN201210525287.0A CN201210525287A CN102965286B CN 102965286 B CN102965286 B CN 102965286B CN 201210525287 A CN201210525287 A CN 201210525287A CN 102965286 B CN102965286 B CN 102965286B
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- bar magnet
- culture vessel
- culture
- screw micrometer
- nutrient solution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- Bioinformatics & Cheminformatics (AREA)
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- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
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- General Health & Medical Sciences (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a magnetically-driven sliding load device capable of controlling the compression amount. The device comprises a culture vessel, a vessel sealing cover, two culture solution conduits, a spiral micrometer, a spiral micrometer measuring bar, a stop nut, a rotary magnetic bar and an external magnetic field, wherein the two culture solution conduits penetrate into the bottom of the culture vessel; a culture solution is provided by a peristaltic pump; the spiral micrometer measuring bar penetrates into the culture vessel and the penetration height is adjusted through the spiral micrometer; the lower end of the spiral micrometer measuring bar is provided with a journal and is in sliding fit with the rotary magnetic bar; an external magnetic field is located outside the culture vessel and used for providing rotary power to the rotary magnetic bar; a ring-shaped culture is placed between the lower surface of the rotary magnetic bar and the upper surface of the bottom of the culture vessel. The magnetically-driven sliding load device has the advantages of being simple in structure, easy in operation and convenient in cleaning and disinfection, avoiding the culture from being polluted by using magnetic force as power to separate the power source from the load components, and being high in precision, small in volume and easy in control by using the spiral micrometer to control the sliding load compression amount.
Description
[technical field]
The present invention relates generally to biomedical engineering field, particularly a kind of magnetic force driving slip deceleration loading device of controlled draught processed.
[background technology]
Organizational project, refers to principle and method by engineering science and life science, and preparation tissue and organ surrogate, to recover to maintain or improve the function of human tissue organ, are rapid far-reaching biomedical engineering Application Areass of development.At present, tissue engineering skin product formally enters people's clinical application, and organizational project bone, cartilage, skin and the nervous tissue of cultivation carried out experiment in body.Organizational project bio-reactor be bring up in vitro one to similar environment in body, accelerate the system of culturing cell, tissue and even organ, can be widely used in substituting, build, keeping or strengthen the fields such as its function of organization and bio-pharmaceuticals as organ defect patient.Development along with organizational project, deepening continuously of seed cell, timbering material and active factor research, in bio-reactor, how to cultivate graft and receive more concern, the mechanics problem of wherein studying mechanics under external environment, biochemical factors and how to affect dimensional culture and functionalization, the especially carrier tissue of cell is significant for the development of organizational project.Recent years, correlative study person did large quantity research to the structure and function of different mechanical stimulations and optimization culture, and had researched and developed targetedly many advanced persons' organizational project bio-reactor.And aspect slip loading, organizational project bio-reactor is actually rare, based on above factor, our magnetic force driving slip deceleration loading device is intended to load mechanical condition for tissue provides a kind of slip.
[summary of the invention]
The object of the invention is for above-mentioned existing problems, a kind of slip deceleration loading device of controlled draught processed is provided, this slip deceleration loading device is simple in structure, easy handling, can prevent the pollution of propulsion source to culture, is convenient to device to clean, disinfect.
Technical scheme of the present invention:
A kind of magnetic force driving slip deceleration loading device of controlled draught processed, comprise culture vessel, airtight lid for container, nutrient solution conduit, screw micrometer, screw micrometer measuring staff, stop nut, rotation bar magnet and foreign field, the both sides of airtight lid for container are provided with two symmetrical circular holes, two nutrient solution conduits are deep into respectively the bottom of culture vessel through two symmetrical circular holes, two nutrient solution conduits are fixed with airtight lid for container respectively, nutrient solution conduit provides nutrient solution by peristaltic pump, and nutrient solution conduit is provided with catheter stopper, screw micrometer is fixed on airtight lid for container upper surface center, screw micrometer measuring staff is deep in culture vessel and by screw micrometer and regulates deeply height, the lower end of screw micrometer measuring staff is provided with axle journal, the semi-cylindrical that the cross section of rotating bar magnet is semicircle and rotation bar magnet is lower surface, rotate bar magnet and be provided with centre hole, rotating bar magnet is connected and forms with the axle journal of screw micrometer measuring staff by centre hole and be slidably matched, the termination of screw micrometer measuring staff is provided with stop nut, foreign field is positioned at the outside of culture vessel and provides rotational power for rotating bar magnet, circular culture is placed in and rotates at the bottom of bar magnet lower surface and culture vessel device between upper surface.
Advantage of the present invention is: this magnetic force driving slip deceleration loading device is simple in structure, easy handling, be convenient to cleanse and disinfect and process; Adopt magnetic force as power, propulsion source and loading component isolation, avoided culture to be polluted; Adopt screw micrometer to control to slide to load draught, precision is high, volume is little, easy to control.
[accompanying drawing explanation]
Fig. 1 is this magnetic force driving slip deceleration loading device structural representation.
Fig. 2 is for rotating bar magnet perspective view.
In figure: 1. culture vessel 2. airtight lid for container 3. nutrient solution conduit 4. screw micrometer 5. screw micrometer measuring staff 6. stop nuts 7. rotate bar magnet 8. foreign field 9. nutrient solution 10. catheter stopper 11. neck axle 12. cultures
[embodiment]
Embodiment:
A kind of magnetic force driving slip deceleration loading device, comprise culture vessel 1, airtight lid for container 2, nutrient solution conduit 3, screw micrometer 4, screw micrometer measuring staff 5, stop nut 6, rotate bar magnet 7 and foreign field 8, the both sides of airtight lid for container 2 are provided with two symmetrical circular holes, two nutrient solution conduits 3 are deep into respectively the bottom of culture vessel 1 through two symmetrical circular holes, two nutrient solution conduits 3 seal with two symmetrical circular holes respectively, nutrient solution conduit 3 provides nutrient solution 9 by peristaltic pump, and nutrient solution conduit 3 is provided with catheter stopper 10, screw micrometer 5 is fixed on airtight lid for container 2 upper surface centers, screw micrometer measuring staff 6 is deep in culture vessel 1 and by screw micrometer 4 and regulates deeply height, the lower end of screw micrometer measuring staff 5 is provided with neck axle 11, the semi-cylindrical that the cross section of rotating bar magnet 7 is semicircle and rotation bar magnet 7 is lower surface, rotate bar magnet 7 and be provided with centre hole, rotating bar magnet is connected and forms with the axle journal of screw micrometer measuring staff 5 by centre hole and be slidably matched, the termination of screw micrometer measuring staff 5 is provided with stop nut 6, foreign field 8 is positioned at the outside of round culture vessel 1 and provides rotational power for rotating bar magnet 7, the circular culture 11 of magnetic force is placed in and rotates at the bottom of bar magnet 7 lower surfaces and culture vessel 1 device between upper surface.
In this embodiment, the range of screw micrometer (claiming again milscale) is that 0-25mm, precision are that 0.01mm, Shanghai Measuring and Cutting Tools Plant produce; Foreign field is rotated and is formed by the bar shaped bar magnet being connected with electric motor, and the rotation of bar shaped bar magnet drives the rotation bar magnet in culture vessel to rotate thereupon.
The working routine of this embodiment:
Screw micrometer and the container cover of this magnetic force driving slip deceleration loading device are integrated; Rotate the lower end of bar magnet and limited by stop nut and measuring staff afterbody bolt, rotating bar magnet can and can not move up and down around the rotation of screw micrometer measuring staff on measuring staff; Conduit can be connected with peristaltic pump, to change the nutrient solution in container, while normally working, by catheter stopper, is sealed.When the lower end of rotation bar magnet is positioned at bottommost, the reading of setting screw micrometer is zero; Rotate under the magneticaction of bar magnet outside magnetic field and rotate in container, realize the slip of culture is loaded, control screw micrometer measuring staff so that rotate that the lower surface of bar magnet contacts with culture and the reading of screw micrometer is now designated as to A; If it is that the slip of B loads that culture is applied to draught, the deep height of adjusting screw micrometer measuring staff, making its reading is the difference of A and B, thereby has realized the slip loading accurate controlled to culture draught.
Practice shows: this magnetic force driving slip deceleration loading device is respond well, reaches technical requirements completely.
Claims (1)
1. the magnetic force driving slip deceleration loading device of a controlled draught processed, it is characterized in that: comprise culture vessel, airtight lid for container, nutrient solution conduit, screw micrometer, screw micrometer measuring staff, stop nut, rotation bar magnet and foreign field, the both sides of airtight lid for container are provided with two symmetrical circular holes, two nutrient solution conduits are deep into respectively the bottom of culture vessel through two symmetrical circular holes, two nutrient solution conduits are fixed with airtight lid for container respectively, nutrient solution conduit provides nutrient solution by peristaltic pump, and nutrient solution conduit is provided with catheter stopper, screw micrometer is fixed on airtight lid for container upper surface center, screw micrometer measuring staff is deep in culture vessel and by screw micrometer and regulates deeply height, the lower end of screw micrometer measuring staff is provided with axle journal, the semi-cylindrical that the cross section of rotating bar magnet is semicircle and rotation bar magnet is lower surface, rotate bar magnet and be provided with centre hole, rotating bar magnet is connected and forms with the axle journal of screw micrometer measuring staff by centre hole and be slidably matched, the termination of screw micrometer measuring staff is provided with stop nut, foreign field is positioned at the outside of culture vessel and provides rotational power for rotating bar magnet, circular culture is placed in and rotates at the bottom of bar magnet lower surface and culture vessel device between upper surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210525287.0A CN102965286B (en) | 2012-12-06 | 2012-12-06 | Magnetically-driven sliding load device capable of controlling compression amount |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210525287.0A CN102965286B (en) | 2012-12-06 | 2012-12-06 | Magnetically-driven sliding load device capable of controlling compression amount |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102965286A CN102965286A (en) | 2013-03-13 |
| CN102965286B true CN102965286B (en) | 2014-04-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210525287.0A Expired - Fee Related CN102965286B (en) | 2012-12-06 | 2012-12-06 | Magnetically-driven sliding load device capable of controlling compression amount |
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Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1953220A1 (en) * | 2007-02-01 | 2008-08-06 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Method for applying mechanical loading to cells and apparatus for implementing such method |
| CN101298592B (en) * | 2008-06-16 | 2011-06-01 | 重庆大学 | Cell three-dimensional mechanical loading unit |
| CN201762331U (en) * | 2010-08-31 | 2011-03-16 | 北京清大天一科技有限公司 | Magnetic stirring device and animal cell culture bioreactor |
| CN101979495B (en) * | 2010-09-08 | 2013-06-19 | 上海国强生化工程装备有限公司 | Magnetic stirring bioreactor and magnetic stirring system |
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Granted publication date: 20140423 Termination date: 20141206 |
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