CN102965286A - Magnetically-driven sliding load device capable of controlling compression amount - Google Patents
Magnetically-driven sliding load device capable of controlling compression amount Download PDFInfo
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- CN102965286A CN102965286A CN2012105252870A CN201210525287A CN102965286A CN 102965286 A CN102965286 A CN 102965286A CN 2012105252870 A CN2012105252870 A CN 2012105252870A CN 201210525287 A CN201210525287 A CN 201210525287A CN 102965286 A CN102965286 A CN 102965286A
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- bar magnet
- culture vessel
- culture
- screw micrometer
- nutrient solution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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Abstract
The invention discloses a magnetically-driven sliding load device capable of controlling the compression amount. The device comprises a culture vessel, a vessel sealing cover, two culture solution conduits, a spiral micrometer, a spiral micrometer measuring bar, a stop nut, a rotary magnetic bar and an external magnetic field, wherein the two culture solution conduits penetrate into the bottom of the culture vessel; a culture solution is provided by a peristaltic pump; the spiral micrometer measuring bar penetrates into the culture vessel and the penetration height is adjusted through the spiral micrometer; the lower end of the spiral micrometer measuring bar is provided with a journal and is in sliding fit with the rotary magnetic bar; an external magnetic field is located outside the culture vessel and used for providing rotary power to the rotary magnetic bar; a ring-shaped culture is placed between the lower surface of the rotary magnetic bar and the upper surface of the bottom of the culture vessel. The magnetically-driven sliding load device has the advantages of being simple in structure, easy in operation and convenient in cleaning and disinfection, avoiding the culture from being polluted by using magnetic force as power to separate the power source from the load components, and being high in precision, small in volume and easy in control by using the spiral micrometer to control the sliding load compression amount.
Description
[technical field]
The present invention relates generally to biomedical engineering field, particularly a kind of magnetic force driving slip deceleration loading device of controlled draught processed.
[background technology]
Organizational project refers to principle and method with engineering science and life science, and preparation tissue and organ surrogate to recover keeping or the improve function of human tissue organ, are rapid far-reaching biomedical engineering Application Areass of development.At present, the tissue engineering skin product formally advances people's clinical application, and organizational project bone, cartilage, skin and the nervous tissue of cultivation carried out experiment in the body.The organizational project bio-reactor is environment similar in one of external achievements and body, accelerate the system of culturing cell, tissue and even organ, can be widely used in substituting, make up, keeping or strengthen the fields such as its function of organization and bio-pharmaceuticals such as the organ defect patient.Development along with organizational project, deepening continuously of seed cell, timbering material and active factor research, in bio-reactor, how to cultivate graft and receive more concern, the mechanics problem of wherein studying mechanics under the external environment, biochemical factors and how to affect dimensional culture and functionalization, the especially carrier tissue of cell is significant for the development of organizational project.Recent years, correlative study person did large quantity research to the structure and function of different mechanical stimulations and optimization culture, and had researched and developed targetedly many advanced persons' organizational project bio-reactor.And aspect the slip loading, the organizational project bio-reactor is actually rare, and based on above factor, our magnetic force driving slip deceleration loading device is intended to load mechanical condition for tissue provides a kind of slip.
[summary of the invention]
The objective of the invention is for above-mentioned existing problems, a kind of slip deceleration loading device of controlled draught processed is provided, simple in structure, the easy handling of this slip deceleration loading device can prevent propulsion source to the pollution of culture, is convenient to device is cleaned, disinfects.
Technical scheme of the present invention:
A kind of magnetic force driving slip deceleration loading device of controlled draught processed, comprise culture vessel, airtight lid for container, nutrient solution conduit, screw micrometer, screw micrometer measuring staff, stop nut, rotation bar magnet and foreign field, the both sides of airtight lid for container are provided with two symmetrical circular holes, two nutrient solution conduits pass respectively the bottom that two symmetrical circular holes are deep into culture vessel, two nutrient solution conduits are fixed with airtight lid for container respectively, the nutrient solution conduit provides nutrient solution by peristaltic pump, and the nutrient solution conduit is provided with catheter stopper; Screw micrometer is fixed in airtight lid for container upper surface center, the screw micrometer measuring staff is deep in the culture vessel and by screw micrometer and regulates deeply height, the lower end of screw micrometer measuring staff is provided with axle journal, the cross section of rotating bar magnet is lower surface for semi-cylindrical semicircle and the rotation bar magnet, rotate bar magnet and be provided with centre hole, rotating bar magnet is connected and forms with the axle journal of screw micrometer measuring staff by centre hole and be slidingly matched, the termination of screw micrometer measuring staff is provided with stop nut, foreign field is positioned at the outside of culture vessel and provides rotational power for rotating bar magnet, and circular culture places and rotates at the bottom of bar magnet lower surface and the culture vessel device between the upper surface.
Advantage of the present invention is: this magnetic force driving slip deceleration loading device is simple in structure, easy handling, be convenient to cleanse and disinfect and process; Adopt magnetic force as power, propulsion source and loading component isolation have avoided culture to be polluted; Adopt screw micrometer control to slide and load draught, precision is high, volume is little, easy to control.
[description of drawings]
Fig. 1 is this magnetic force driving slip deceleration loading device structural representation.
Fig. 2 is for rotating the bar magnet perspective view.
Among the figure: 1. culture vessel 2. airtight lid for container 3. nutrient solution conduits 4. screw micrometers 5. screw micrometer measuring staffs 6. stop nuts 7. rotate bar magnet 8. foreign fields 9. nutrient solutions 10. catheter stopper 11. neck axles 12. cultures
[embodiment]
Embodiment:
A kind of magnetic force driving slip deceleration loading device, comprise culture vessel 1, airtight lid for container 2, nutrient solution conduit 3, screw micrometer 4, screw micrometer measuring staff 5, stop nut 6, rotate bar magnet 7 and foreign field 8, the both sides of airtight lid for container 2 are provided with two symmetrical circular holes, two nutrient solution conduits 3 pass respectively the bottom that two symmetrical circular holes are deep into culture vessel 1, two nutrient solution conduits 3 seal with two symmetrical circular holes respectively, nutrient solution conduit 3 provides nutrient solution 9 by peristaltic pump, and nutrient solution conduit 3 is provided with catheter stopper 10; Screw micrometer 5 is fixed in airtight lid for container 2 upper surface centers, screw micrometer measuring staff 6 is deep in the culture vessel 1 and by screw micrometer 4 and regulates deeply height, the lower end of screw micrometer measuring staff 5 is provided with neck axle 11, the cross section of rotating bar magnet 7 is lower surface for semi-cylindrical semicircle and rotation bar magnet 7, rotate bar magnet 7 and be provided with centre hole, rotating bar magnet is connected and forms with the axle journal of screw micrometer measuring staff 5 by centre hole and be slidingly matched, the termination of screw micrometer measuring staff 5 is provided with stop nut 6, foreign field 8 is positioned at the outside of round culture vessel 1 and provides rotational power for rotating bar magnet 7, and the circular culture 11 of magnetic force places and rotates at the bottom of bar magnet 7 lower surfaces and culture vessel 1 device between the upper surface.
Among this embodiment, the range of screw micrometer (claiming again milscale) is that 0-25mm, precision are that 0.01mm, Shanghai Measuring and Cutting Tools Plant produce; Foreign field is rotated by the bar shaped bar magnet that is connected with electric motor and is formed, and the rotation bar magnet that the rotation of bar shaped bar magnet drives in the culture vessel rotates thereupon.
The working routine of this embodiment:
Screw micrometer and the container cover of this magnetic force driving slip deceleration loading device are integrated; Rotate the lower end of bar magnet by stop nut and the restriction of measuring staff afterbody bolt, rotating bar magnet can and can not move up and down on measuring staff around the rotation of screw micrometer measuring staff; Conduit can be connected with peristaltic pump, in order to change the nutrient solution in the container, is sealed by catheter stopper during normal operation.When the lower end of rotating bar magnet was positioned at bottommost, the reading of setting screw micrometer was zero; Rotate under the magneticaction of bar magnet outside magnetic field and rotate in container, realize the slip of culture is loaded, control screw micrometer measuring staff is so that rotate that the lower surface of bar magnet contact with culture and the reading of screw micrometer at this moment is designated as A; If it is that the slip of B loads that culture is applied draught, the deep height of adjusting screw micrometer measuring staff then, making its reading is that the difference of A and B get final product, thereby has realized the slip loading accurate controlled to the culture draught.
Practice shows: this magnetic force driving slip deceleration loading device is respond well, reaches technical requirements fully.
Claims (1)
1. the magnetic force driving slip deceleration loading device of a controlled draught processed, it is characterized in that: comprise culture vessel, airtight lid for container, nutrient solution conduit, screw micrometer, screw micrometer measuring staff, stop nut, rotation bar magnet and foreign field, the both sides of airtight lid for container are provided with two symmetrical circular holes, two nutrient solution conduits pass respectively the bottom that two symmetrical circular holes are deep into culture vessel, two nutrient solution conduits are fixed with airtight lid for container respectively, the nutrient solution conduit provides nutrient solution by peristaltic pump, and the nutrient solution conduit is provided with catheter stopper; Screw micrometer is fixed in airtight lid for container upper surface center, the screw micrometer measuring staff is deep in the culture vessel and by screw micrometer and regulates deeply height, the lower end of screw micrometer measuring staff is provided with axle journal, the cross section of rotating bar magnet is lower surface for semi-cylindrical semicircle and the rotation bar magnet, rotate bar magnet and be provided with centre hole, rotating bar magnet is connected and forms with the axle journal of screw micrometer measuring staff by centre hole and be slidingly matched, the termination of screw micrometer measuring staff is provided with stop nut, foreign field is positioned at the outside of culture vessel and provides rotational power for rotating bar magnet, and circular culture places and rotates at the bottom of bar magnet lower surface and the culture vessel device between the upper surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210525287.0A CN102965286B (en) | 2012-12-06 | 2012-12-06 | Magnetically-driven sliding load device capable of controlling compression amount |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210525287.0A CN102965286B (en) | 2012-12-06 | 2012-12-06 | Magnetically-driven sliding load device capable of controlling compression amount |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102965286A true CN102965286A (en) | 2013-03-13 |
| CN102965286B CN102965286B (en) | 2014-04-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210525287.0A Expired - Fee Related CN102965286B (en) | 2012-12-06 | 2012-12-06 | Magnetically-driven sliding load device capable of controlling compression amount |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1953220A1 (en) * | 2007-02-01 | 2008-08-06 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Method for applying mechanical loading to cells and apparatus for implementing such method |
| CN101298592A (en) * | 2008-06-16 | 2008-11-05 | 重庆大学 | Cell three-dimensional mechanical loading unit |
| CN101979495A (en) * | 2010-09-08 | 2011-02-23 | 上海国强生化工程装备有限公司 | Magnetic stirring bioreactor and magnetic stirring system |
| CN201762331U (en) * | 2010-08-31 | 2011-03-16 | 北京清大天一科技有限公司 | Magnetic stirring device and animal cell culture bioreactor |
-
2012
- 2012-12-06 CN CN201210525287.0A patent/CN102965286B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1953220A1 (en) * | 2007-02-01 | 2008-08-06 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Method for applying mechanical loading to cells and apparatus for implementing such method |
| CN101298592A (en) * | 2008-06-16 | 2008-11-05 | 重庆大学 | Cell three-dimensional mechanical loading unit |
| CN201762331U (en) * | 2010-08-31 | 2011-03-16 | 北京清大天一科技有限公司 | Magnetic stirring device and animal cell culture bioreactor |
| CN101979495A (en) * | 2010-09-08 | 2011-02-23 | 上海国强生化工程装备有限公司 | Magnetic stirring bioreactor and magnetic stirring system |
Non-Patent Citations (1)
| Title |
|---|
| KIFAH SHAHIN ETAL.: "Tissue engineering of cartilage using a mechanobioreactor exerting simultaneous mechanical shear and compression to simulate the rolling action of articular joints", 《BIOTECHNOLOGY AND BIOENGINEERING》 * |
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| CN102965286B (en) | 2014-04-23 |
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