CN217112377U - Sample adding device for NanoSPR detection and microporous plate assembly - Google Patents
Sample adding device for NanoSPR detection and microporous plate assembly Download PDFInfo
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- CN217112377U CN217112377U CN202123184295.7U CN202123184295U CN217112377U CN 217112377 U CN217112377 U CN 217112377U CN 202123184295 U CN202123184295 U CN 202123184295U CN 217112377 U CN217112377 U CN 217112377U
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Abstract
The utility model discloses a sample adding device and a microporous plate component for NanoSPR detection, belonging to the technical field of nano plasma resonance biochip plate detection device equipment; the sample adding device comprises a first plate body, wherein a plurality of first micropores which are arranged in a matrix manner are arranged on the first plate body; the sample loading mechanism comprises an extension part and a bearing part, the extension part extends downwards along the vertical direction, the top of the extension part is connected to the bottom edge of the first micropore or the inner wall of the first micropore, the bottom of the extension part is connected with the bearing part, and a placing table is arranged on the bearing part. The microporous plate component comprises the sample adding device and the microporous plate. Through setting up a kind of application of sample device who is used for nanoSPR biochip board and SPR chip micropore board specially, realized that all micropores add the sample in step, synchronous reaction, be convenient for control sample reaction's time.
Description
Technical Field
The utility model belongs to the technical field of nanometer plasma resonance biochip board detection device equipment, especially a application of sample device and micropore board subassembly that is used for nanometer plasma resonance biochip board to detect.
Background
The microplate reader is a special instrument for enzyme-linked immunosorbent assay, and is also called a microplate detector. Microplates are one of the important components used in microplate readers. The micropore plate is a device made of plastic or other materials, wherein a plurality of micropores are arranged in a matrix in advance, and each micropore is of a closed structure and is transparent at the bottom. The sample to be detected or other necessary reagents can be embedded and placed in the micropores of the microporous plate in advance, and the sample can be temporarily added before detection. The nanometer plasma resonance biochip plate (NanoSPR biochip plate) is one special chip coated with specific antibody or antigen and is used in combining with antigen or antibody in the sample to be detected, detecting with full spectrum enzyme mark instrument and drawing full spectrum. When the microplate reader is used for detection, the general operation steps are that the microplate after sample adding pretreatment is placed on a supporting plate, then the nano plasma resonance biochip plate biochip is inserted into the micropore to enable the chip to react with a sample to be detected, then the probe of the microplate reader is moved and is inserted into the micropore to irradiate the nano plasma resonance biochip plate biochip, and the received reflected light or transmitted light is utilized to detect a sample in the micropore plate.
When the existing microporous plate is used for sample adding, a single-channel or multi-channel pipettor is generally used for sample adding respectively, and when the number of microporous plate channels is large (48 holes, 96 holes, 192 holes and the like), the micropores subjected to sample adding firstly and the micropores subjected to sample adding later have large time difference, so that the synchronous reaction of all the microporous plates can be influenced. Especially, when a plurality of different reagents need to be added in sequence, the influence of time difference caused by the sequential sample addition is more obvious, and the accuracy of the final detection result in each micropore is further influenced.
In order to ensure that the samples in all the micro-porous plates synchronously react and improve the accuracy and precision of the detection result, a device needs to be designed to realize the synchronous unification of the reaction time in all the micro-pores on the micro-porous plates.
Disclosure of Invention
In order to solve the problems of the prior art, the utility model provides a sample adding device for detecting NanoSPR biochip board to and corresponding micropore board subassembly, through placing the sample or the reagent sample adding that will experimental needs on the appearance mechanism that carries that every micropore corresponds earlier, then through rocking or shake realize that all micropore's sample or reagent fall into the micropore in step and begin to react. The method is realized by the following technical scheme.
The sample adding device for detecting the NanoSPR biochip plate comprises a first plate body, wherein a plurality of first micropores arranged in a matrix manner are arranged on the first plate body; the sample loading mechanism comprises an extension part and a bearing part, the extension part extends downwards along the vertical direction, the top of the extension part is connected to the bottom edge of the first micropore or the inner wall of the first micropore, the bottom of the extension part is connected with the bearing part, and a placing table is arranged on the bearing part.
The sample adding device is specially applied to SPR detection and NanoSPR biochip plate detection, and the using method comprises the following steps: covering the sample adding device on the microporous plate, wherein each first micropore corresponds to a micropore on the microporous plate one by one, and the bearing part of the sample loading mechanism extends into the micropore on the microporous plate; at the moment, the extension part of the sample loading mechanism is vertically arranged, and the placing platform of the bearing part is used for bearing a sample to be tested or a test reagent; the sample to be detected or the test reagent are respectively placed on the placing table, after the sample adding of all the micropores on the microporous plate is finished, the communicating sample adding device and the microporous plate vibrate left and right or rock together, so that the sample to be detected or the test reagent placed on the placing table falls into the micropores of the microporous plate, and then subsequent further detection processing is carried out.
By adopting the sample adding device, all the micropores on the micropore plate can be synchronously added with all samples or reagents, and the reaction time in the micropores can be conveniently and uniformly controlled. The sample adding device is simple in structure and convenient to operate.
Preferably, the extension part is of an elongated or long plate-shaped structure.
Preferably, the table top of the standing table is arranged obliquely relative to the horizontal plane, and the inclination angle alpha is not more than 10 degrees. When the amount of the sample or the reagent added is very small or the viscosity is high, the surface tension and the viscous resistance of the liquid may cause the sample or the reagent to adhere to the placing table and not to be easily shaken off. Therefore, the table top of the placing table is set to be a slightly inclined angle, so that the sample or the reagent can be temporarily stopped on the placing table and can be conveniently vibrated and shaken off subsequently.
Preferably, the number of the sample loading mechanisms is 2-4. For some special detection requirements, more than one sample or experiment reagent may be required, so that a plurality of sample loading mechanisms may be provided, and each sample loading mechanism is provided with 1 sample or experiment reagent individually, so that all the samples and the experiment reagents can be shaken and dropped into the micropores together when vibrating.
The invention also provides a microporous plate assembly for detecting the NanoSPR biochip plate, which comprises a second plate body and any one of the sample adding devices, wherein the second plate body is provided with a plurality of second micropores arranged in a matrix manner, the first plate body covers the second plate body, and the second micropores correspond to the first micropores one to one; the extension part and the bearing part of the sample loading mechanism extend into the second micropore, and the bearing part is suspended above the bottom of the second micropore.
Generally, the bottom of a micropore plate assembly for detecting a NanoSPR biochip plate is mostly assembled and sealed by a transparent glass plate or an acrylic plate, so that a probe of a microplate reader can conveniently irradiate the microfluidic chip in the micropore from the bottom or the top.
Preferably, the first plate body is connected with the second plate body through a rotating member. The rotating member may be a conventional flexible plastic material connector or may be other conventional hinge-type structure.
Preferably, the first plate body and the second plate body are covered up and down through a limiting part.
More preferably, the limiting part comprises a limiting column and a limiting groove which are matched with each other, the limiting column is arranged on the lower surface of the first plate body and/or the upper surface of the second plate body, and the limiting groove corresponding to the limiting column is arranged on the upper surface of the second plate body and/or the lower surface of the first plate body.
More preferably, the limiting part comprises steps arranged on the periphery of the second plate body and protruding parts arranged on the periphery of the first plate body, and the steps are matched with the protruding parts.
Compared with the prior art, the beneficial effects of the utility model are that: through setting up a kind of application of sample device who is used for nanoSPR biochip board and SPR chip micropore board specially, realized that all micropores add the sample in step, synchronous reaction, be convenient for control sample reaction's time.
Drawings
FIG. 1 is a front view of the internal structure of a sample application device for NanoSPR biochip plate detection provided in example 1;
FIG. 2 is a bottom view of the sample application device for NanoSPR biochip plate detection provided in example 1;
FIG. 3 is a front view of the microplate assembly for NanoSPR biochip plate detection provided in example 1;
FIG. 4 is a front view of the internal structure of the sample application device for NanoSPR biochip plate detection provided in example 1;
FIG. 5 is a schematic view of a portion of the structure of FIG. 4 at A;
FIG. 6 is a front view of the microplate assembly for NanoSPR biochip plate assay provided in example 2;
in the figure: 1. a first plate body; 2. a first micropore; 3. an extension portion; 4. a bearing part; 5. a placing table; 6. a second plate body; 7. a second micro-hole; 8. and a rotating member.
Detailed Description
The technical solutions of the embodiments in this patent will be described clearly and completely with reference to the accompanying drawings, and it is obvious that the described embodiments are only some embodiments, not all embodiments, of this patent. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the patent without making creative efforts, shall fall within the protection scope of the patent.
In the description of this patent, it is noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", "top", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, merely to facilitate the description of the patent and to simplify the description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the patent. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of this patent, it is noted that, unless expressly stated or limited otherwise, the terms "mounted," "connected," and "in communication with" are to be construed broadly and include, for example, fixed connections, removable connections, or integral connections; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. It is to be noted that all the figures are exemplary representations. The meaning of the above terms in this patent may be specifically understood by those of ordinary skill in the art.
The patent is described in further detail below with reference to specific embodiments and with reference to the attached drawings.
Example 1
As shown in fig. 1-3, the sample adding device for detecting a NanoSPR biochip plate provided in this embodiment includes a first plate 1, wherein a plurality of first micro-holes 2 arranged in a matrix are disposed on the first plate 1; the sample loading device is further provided with 3 sample loading mechanisms, each sample loading mechanism comprises an extension part 3 and a bearing part 4, the extension parts 3 extend downwards along the vertical direction, the tops of the extension parts 3 are connected to the bottom edges of the first micropores 2, the bottoms of the extension parts 3 are connected with the bearing parts 4, and the bearing parts 4 are provided with placing platforms 5; the extension part 3 is of a long strip structure.
In this embodiment, the sample adding device is utilized in combination, and a corresponding microporous plate assembly is further provided, including a second plate body 6 and the sample adding device, wherein a plurality of second micropores 7 arranged in a matrix are disposed on the second plate body 6, the first plate body 1 covers the second plate body 6, and the second micropores 7 correspond to the first micropores 2 one to one; the extension part 3 and the bearing part 4 of the sample loading mechanism extend into the second micropore 7, and the bearing part 4 is suspended above the bottom of the second micropore 7; the first plate 1 is connected with the second plate 6 through a rotating member 8.
The sample adding device provided by the embodiment is specially applied to SPR detection and NanoSPR biochip plate detection, and the using method comprises the following steps: covering the sample adding device on the microporous plate, wherein each first micropore corresponds to a micropore on the microporous plate one by one, and the bearing part of the sample loading mechanism extends into the micropore on the microporous plate; at the moment, the extension part of the sample loading mechanism is vertically arranged, and the placing platform of the bearing part is used for bearing a sample to be tested or a test reagent; the sample to be detected or the test reagent are respectively placed on the placing table, after the sample adding of all the micropores on the microporous plate is finished, the communicating sample adding device and the microporous plate vibrate left and right or rock together, so that the sample to be detected or the test reagent placed on the placing table falls into the micropores of the microporous plate, and then subsequent further detection processing is carried out.
By adopting the sample adding device, all the micropores on the micropore plate can be synchronously added with all samples or reagents, and the reaction time in the micropores can be conveniently and uniformly controlled. The sample adding device is simple in structure and convenient to operate.
Example 2
As shown in fig. 4-6, the sample adding device for detecting a NanoSPR biochip plate according to the present embodiment is different from embodiment 1 in that the top of the extension part 3 is connected to the inner wall of the first micro well 2, the top of the placing table 5 is disposed to be inclined with respect to the horizontal plane, and the inclination angle α is about 5 °;
in the micropore board assembly that provides, first plate body 1 closes through spacing portion upper and lower lid with second plate body 6, spacing portion is including the spacing post and the spacing groove of mutual matching, spacing post sets up the lower surface of first plate body 1, with it correspond the spacing groove sets up the upper surface of second plate body 6.
The above embodiments describe the implementation of the present invention in detail, however, the present invention is not limited to the specific details of the above embodiments. Within the scope of the claims and the technical idea of the present invention, various simple modifications and changes can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.
Claims (9)
1. The sample adding device for the NanoSPR detection is characterized by comprising a first plate body, wherein a plurality of first micropores which are arranged in a matrix manner are arranged on the first plate body; the sample loading mechanism comprises an extension part and a bearing part, the extension part extends downwards along the vertical direction, the top of the extension part is connected to the bottom edge of the first micropore or the inner wall of the first micropore, the bottom of the extension part is connected with the bearing part, and a placing table is arranged on the bearing part.
2. The sample application device for NanoSPR detection according to claim 1, wherein the extension part is an elongated or long plate-shaped structure.
3. The sample addition device for NanoSPR detection according to claim 1, wherein the top of the placement stage is disposed to be inclined with respect to a horizontal plane, and the inclination angle α is not more than 10 °.
4. The sample application device for NanoSPR detection according to claim 1, wherein the number of sample application mechanisms is 2 to 4.
5. A microplate assembly for NanoSPR detection, comprising a second plate body and the sample adding device for NanoSPR biochip plate detection as claimed in any one of claims 1 to 4, wherein the second plate body is provided with a plurality of second micropores arranged in a matrix manner, and the first plate body covers the second plate body, and the second micropores correspond to the first micropores one to one; the extension part and the bearing part of the sample loading mechanism extend into the second micropore, and the bearing part is suspended above the bottom of the second micropore.
6. The microplate assembly of claim 5, wherein the first plate body and the second plate body are connected by a rotating member.
7. The microplate assembly for NanoSPR detection of claim 5, wherein the first plate body and the second plate body are closed up and down by a limiting portion.
8. The microplate assembly for NanoSPR detection according to claim 7, wherein the limiting portion comprises a limiting post and a limiting groove which are matched with each other, the limiting post is disposed on the lower surface of the first plate and/or the upper surface of the second plate, and the corresponding limiting groove is disposed on the upper surface of the second plate and/or the lower surface of the first plate.
9. The microplate assembly as claimed in claim 7, wherein the limiting portion comprises a step disposed at a peripheral edge of the second plate body and a protrusion disposed at a peripheral edge of the first plate body, the step matching the protrusion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123184295.7U CN217112377U (en) | 2021-12-17 | 2021-12-17 | Sample adding device for NanoSPR detection and microporous plate assembly |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202123184295.7U CN217112377U (en) | 2021-12-17 | 2021-12-17 | Sample adding device for NanoSPR detection and microporous plate assembly |
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| CN217112377U true CN217112377U (en) | 2022-08-02 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118243512A (en) * | 2024-05-29 | 2024-06-25 | 常州市昌隆电机股份有限公司 | Intensity detection device of motor shaft casting |
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2021
- 2021-12-17 CN CN202123184295.7U patent/CN217112377U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118243512A (en) * | 2024-05-29 | 2024-06-25 | 常州市昌隆电机股份有限公司 | Intensity detection device of motor shaft casting |
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