CN217085002U - Micro-fluidic detection chip - Google Patents
Micro-fluidic detection chip Download PDFInfo
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- CN217085002U CN217085002U CN202220616177.4U CN202220616177U CN217085002U CN 217085002 U CN217085002 U CN 217085002U CN 202220616177 U CN202220616177 U CN 202220616177U CN 217085002 U CN217085002 U CN 217085002U
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Abstract
The utility model discloses a micro-fluidic detection chip, which is provided with a bottom plate, a filtering membrane and an upper cover from bottom to top in sequence; the upper surface of the bottom plate is provided with a liquid inlet groove with gradually changed depth, and two sides of the liquid inlet groove are provided with connecting grooves; one end of the connecting groove, which is far away from the liquid inlet groove, is provided with a detection hole; the upper cover is provided with a liquid inlet hole corresponding to the liquid inlet groove. The utility model discloses a micro-fluidic detection chip through the feed liquor groove, spread groove and the inspection hole of degree of depth gradual change for the device sample can be landing downwards under the effect of gravity when using, at the in-process of landing alright enter into the inspection hole. The conventional negative pressure generator is not needed, the device structure is simpler, and the use is more convenient.
Description
Technical Field
The utility model relates to a micro-fluidic detection area especially relates to a micro-fluidic detection chip.
Background
Microfluidics refers to the science and technology involved in systems that process or manipulate tiny fluids using microchannels, and is an emerging interdiscipline that involves chemical, fluid physics, microelectronics, new materials, biology, and biomedical engineering. Because of their miniaturization, integration, etc., microfluidic devices are commonly referred to as microfluidic chips, also known as lab-on-a-chip and micro total analysis systems.
In the prior art, a patent with the application number of "202120261630. X", entitled "microfluidic-based detection chip" discloses a microfluidic detection chip, which mainly extracts gas inside the device through negative pressure, so that liquid can enter into a detection well for detection. But the mode that it adopted negative pressure generator to extract makes this detection chip comparatively complicated when using, and the operation is inconvenient.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a micro-fluidic detection chip compares in the detection of adopting negative pressure generator extraction mode among the prior art, and the structure is more simple, and it is more convenient to operate.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a microfluidic detection chip is provided with a bottom plate, a filtering membrane and an upper cover in sequence from bottom to top; the upper surface of the bottom plate is provided with a liquid inlet groove with gradually changed depth, and two sides of the liquid inlet groove are provided with connecting grooves; one end of the connecting groove, which is far away from the liquid inlet groove, is provided with a detection hole; the upper cover is provided with a liquid inlet hole corresponding to the liquid inlet groove.
Optionally, the connecting groove is a groove with gradually changing depth, and the depth of the connecting groove far away from one end of the liquid inlet groove is larger than the depth of the connecting groove near one end of the liquid inlet groove.
Optionally, the number of the connecting grooves is multiple.
Optionally, the upper surface of the bottom plate is further provided with a collecting hole, and the collecting hole is located at the tail of the liquid inlet tank.
Optionally, the collection hole is internally provided with a discharge hole with a circular structure.
Optionally, a sealing groove is formed in the edge of the upper surface of the bottom plate, and a sealing protrusion corresponding to the sealing groove is arranged on the edge of the lower surface of the upper cover.
Optionally, a rubber ring is arranged in the sealing groove.
Optionally, the detection hole is used for accommodating a fluorescent antibody, and the fluorescent antibody is any one of fluorescein isothiocyanate, rhodamine fluorescein and dichlorotriazinylaminofluorescein.
The utility model discloses following beneficial effect has:
the utility model discloses a micro-fluidic detection chip through the feed liquor groove, spread groove and the inspection hole of degree of depth gradual change for the device sample can be landing downwards under the effect of gravity when using, alright enter into the inspection hole at the in-process of landing. The conventional negative pressure generator is not needed, the device structure is simpler, and the use is more convenient.
Drawings
Fig. 1 is a schematic diagram of an overall structure of a microfluidic detection chip provided in this embodiment;
fig. 2 is an exploded view of a microfluidic chip according to the present embodiment;
fig. 3 is an exploded view of the microfluidic chip according to the present embodiment from a second perspective;
fig. 4 is a schematic diagram of a bottom plate structure of the microfluidic detection chip provided in this embodiment;
fig. 5 is a cross-sectional view of a bottom plate structure of the microfluidic detection chip provided in this embodiment.
Illustration of the drawings:
1. a base plate; 2. a liquid inlet tank; 3. a sample dispersion chamber; 4. connecting grooves; 5. a detection hole; 6. a collection well; 7. a discharge hole; 8. a second sealing plug; 9. sealing the groove; 10. a rubber ring; 11. an upper cover; 12. sealing the bump; 13. a liquid inlet hole; 14. a first sealing plug; 15. and (4) filtering the membrane.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The terms "first," "second," "third," and the like in the description and in the claims, as well as in the drawings, if any, are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the objects so described are interchangeable under appropriate circumstances. Furthermore, the terms "comprising" and "having," as well as any variations thereof, are intended to cover non-exclusive inclusions.
In the present disclosure, the drawings discussed below and the various embodiments used to describe the principles of the present disclosure are by way of illustration only and should not be construed to limit the scope of the disclosure. Those skilled in the art will understand that the principles of the present invention may be implemented in any suitably arranged system. Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. Further, a terminal according to an exemplary embodiment will be described in detail with reference to the accompanying drawings. Like reference symbols in the various drawings indicate like elements.
The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the inventive concepts. Unless the context clearly dictates otherwise, expressions used in the singular form encompass expressions in the plural form. In the present specification, it is to be understood that terms such as "comprising," "having," and "containing" are intended to specify the presence of the features, integers, steps, acts, or combinations thereof disclosed in the specification, and are not intended to preclude the presence or addition of one or more other features, integers, steps, acts, or combinations thereof. Like reference symbols in the various drawings indicate like elements.
As shown in fig. 1 to 5, the present embodiment discloses a microfluidic detection chip, which includes a bottom plate 1, a filter membrane 15 and an upper cover 11, which are sequentially disposed from bottom to top. Wherein, the upper surface of the bottom plate 1 is provided with a liquid inlet groove 2 with gradually changed depth, and two sides of the liquid inlet groove 2 are provided with connecting grooves 4. The one end that feed liquor groove 2 was kept away from to spread groove 4 has seted up inspection hole 5. The upper cover 11 is provided with a liquid inlet hole 13 corresponding to the liquid inlet groove 2.
In use, the device is first placed in the detection well 5 with the fluorescent antibody. Then the sample is poured into the liquid inlet hole 13, and the sample flows into the liquid inlet groove 2 of the bottom plate 1 after being filtered by the filter membrane 15. Because the liquid inlet groove 2 is of a structure with gradually changed depth, the sample can flow in the liquid inlet groove 2, and the sample in the liquid inlet groove 2 is dispersed in the sample dispersion chamber 3 and then flows into the connecting groove 4 to be combined with the fluorescent antibody in the detection hole 5. The content of the detection can be judged by observing whether the fluorescence occurs in the detection hole 5.
In this embodiment, the connecting groove 4 is a groove with gradually changed depth, and the depth of the connecting groove 4 far away from the end of the liquid inlet groove 2 is greater than the depth of the connecting groove 4 near the end of the liquid inlet groove 2. In this way, the sample will better flow into the detection well 5 to bind to the fluorescent antibody. Wherein, the connecting groove 4 can be a plurality of, and can place different fluorescence antibody in every connecting groove 4, can realize the detection of more projects.
In this embodiment, the liquid inlet 13 is provided with a first sealing plug 14 inside, and the liquid inlet 13 can be sealed by the first sealing plug 14. The edge of the upper surface of the bottom plate 1 is provided with a sealing groove 9, and a rubber ring 10 is arranged in the sealing groove 9. The edge of the lower surface of the upper cover 11 is provided with a sealing protrusion 12 corresponding to the sealing groove 9. The upper cover 11 can be attached to the sealing groove 9 through the sealing bump 12 to seal the microfluidic detection chip, so that the device is in a sealed state when in use. Thus, the liquid inlet tank 2 can store a part of samples, and the part of samples can slowly enter different connecting tanks 4 under the action of gravity, so that the device can be prevented from being in a sample adding state all the time.
Furthermore, the upper surface of the bottom plate 1 is also provided with a collecting hole 6, and the collecting hole 6 is positioned at the tail part of the liquid inlet groove 2. The collection hole 6 is internally provided with a discharge hole 7 with a circular structure. The collection well 6 and the discharge well 7 are provided to allow the excess sample to flow out. Wherein the interior of the outlet opening 7 is provided with a second sealing plug 8, by means of which the outlet opening 7 can be sealed off.
Further, the fluorescent antibody in the present embodiment is any one of fluorescein isothiocyanate, rhodamine fluorescein and dichlorotriazinylamino fluorescein.
The micro-fluidic chip disclosed in this embodiment is through the feed liquor groove, spread groove and the inspection hole of degree of depth gradual change for the device sample can be landing downwards under the effect of gravity when using, alright enter into the inspection hole at the in-process of landing. The conventional negative pressure generator is not needed, the device structure is simpler, and the use is more convenient.
In the microfluidic detection chip disclosed in this embodiment, the connecting groove is a groove with gradually changing depth, and the depth of the end of the connecting groove far away from the liquid inlet groove is greater than the depth of the end of the connecting groove close to the liquid inlet groove. The sample can better flow into the detection hole to be combined with the fluorescent antibody, and the detection stability is improved.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The principle and the implementation of the present invention are explained by applying specific examples, and the above description of the examples is only used to help understand the core idea of the present invention; meanwhile, for the general technical personnel in the field, according to the idea of the present invention, there are changes in the concrete implementation and the application scope. In summary, the content of the present specification should not be construed as a limitation of the present invention.
Claims (8)
1. The microfluidic detection chip is characterized in that a bottom plate (1), a filtering membrane (15) and an upper cover (11) are sequentially arranged on the microfluidic detection chip from bottom to top; the upper surface of the bottom plate (1) is provided with a liquid inlet groove (2) with gradually changed depth, and two sides of the liquid inlet groove (2) are provided with connecting grooves (4); one end of the connecting groove (4) far away from the liquid inlet groove (2) is provided with a detection hole (5); the upper cover (11) is provided with a liquid inlet hole (13) corresponding to the liquid inlet groove (2).
2. The microfluidic detection chip of claim 1, wherein: the connecting groove (4) is a groove with gradually changed depth, and the depth of one end of the liquid inlet groove (2) far away from the connecting groove (4) is larger than the depth of one end of the connecting groove (4) close to the liquid inlet groove (2).
3. The microfluidic detection chip of claim 1, wherein: the number of the connecting grooves (4) is multiple.
4. The microfluidic detection chip of claim 1, wherein: the upper surface of bottom plate (1) has still seted up collection hole (6), collection hole (6) are located the afterbody of feed liquor groove (2).
5. The microfluidic detection chip of claim 4, wherein: and a discharge hole (7) with a circular structure is formed in the collecting hole (6).
6. The microfluidic detection chip of claim 1, wherein: the edge of the upper surface of the bottom plate (1) is provided with a sealing groove (9), and the edge of the lower surface of the upper cover (11) is provided with a sealing convex block (12) corresponding to the sealing groove (9).
7. The microfluidic detection chip of claim 6, wherein: and a rubber ring (10) is arranged in the sealing groove (9).
8. The microfluidic detection chip of claim 1, wherein: the detection hole (5) is used for accommodating a fluorescent antibody, and the fluorescent antibody is any one of fluorescein isothiocyanate, rhodamine fluorescein and dichlorotriazinyl amino fluorescein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220616177.4U CN217085002U (en) | 2022-03-16 | 2022-03-16 | Micro-fluidic detection chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202220616177.4U CN217085002U (en) | 2022-03-16 | 2022-03-16 | Micro-fluidic detection chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217085002U true CN217085002U (en) | 2022-07-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202220616177.4U Active CN217085002U (en) | 2022-03-16 | 2022-03-16 | Micro-fluidic detection chip |
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| CN (1) | CN217085002U (en) |
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- 2022-03-16 CN CN202220616177.4U patent/CN217085002U/en active Active
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