CN101421627A - Filter-carrying micro plate - Google Patents
Filter-carrying micro plate Download PDFInfo
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- CN101421627A CN101421627A CNA2007800137642A CN200780013764A CN101421627A CN 101421627 A CN101421627 A CN 101421627A CN A2007800137642 A CNA2007800137642 A CN A2007800137642A CN 200780013764 A CN200780013764 A CN 200780013764A CN 101421627 A CN101421627 A CN 101421627A
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- filtrator
- microplate
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Abstract
There is provided a filter-equipped microplate including: an upper container 41 having openings 50 for injection of a substance to be tested; a top packing 44 and a bottom packing 45 that hold filters 46; a middle container 42 that fits with the upper container 41 and has openings 63 through which a test sample that has passed through the filters 46 runs, and that clamps the top packing 44 and the bottom packing 45 against the upper container 41: and a lower container 43 having reservoirs 80 that retain the test sample, the lower container 43 being held in a freely detachable manner against the middle container 42, wherein the middle container 42 has guide walls 64 that suspend down from the openings 63 and provide downward openings 65 to the bottom end, and the reservoirs 80 in the lower container 43 house the guide walls 64, the reservoirs 80 receiving the test sample supplied from the downward openings 65 of the guide walls 64 through the filters 46, and the lower container 43 having vents 90 at a widening slant 92 at the top, that communicate with the outside of the reservoirs 80.
Description
Technical field
The present application relates to a kind of microplate with filtrator.In more detail, relate to having the microplate of the filtrator of following structure built-in, that is, will be examined material and lead in the filtrator, the inspection sample collection that filtered in the portion of retaining, and can easily be separated this and retains portion.The microplate of band filtrator is widely used in cell tissue and cultivates or the inspection of live body cultured tissue and other fields.
Background technology
In the microplate of band filtrator, from be examined material, isolate the composition that hinders detection sensitivity by filtrator in advance, that is to say and filter, only obtain desirable culturing purposes or check useful composition.This be because, under the situation of having sneaked into unwanted composition, exist original undesired peak value to occur too much and the peak point of purpose be difficult for specific, the problem that the peak point of perhaps wanting does not originally suitably react.Only the effective constituent of separating through such filtration is consigned to subsequent processing.
Therefore, in the microplate of such band filtrator, only need before composition content generation rerum natura change after this, from this microplate, to separate rapidly and easily having accommodated the portion that retains that utilizes the effective constituent that filtrator separates or having had the container that this retains portion.Therefore, the container that only just has a portion of retaining unloads from microplate rapidly and easily and just becomes extremely important.And microplate is made of various key elements, also is very important with the state and the rapidly practical assembling of cleaning always with these key elements.
In the past, the microplate of known following band filtrator, it is to comprise the multilayer concavity body apparatus for assembling of test flume, filtrator and sensor up and down in order to eliminate the unnecessary step that is used for cell dyeing, fluorescent sign etc. and cost, and this sensor can detect the object that accumulates the bottom test flume via the method that the filtrator utilization does not have a sign.
And, following this microplate with filtrator etc. is known, promptly, in the unit of having accommodated fluid nutrient medium, supply with specific bacterium via filtrator, when making the microplate vibration in order to promote the cultivation in this nutrient culture media, in order to prevent that this fluid nutrient medium from dispersing and invade in other unit, and be provided with the back up pad that is coated with the cementing agent that prevents this usefulness of dispersing.
But, but not being conceived to above-mentioned viewpoint and working out the following microplate that has filtrator, it has can be with container part easily from the structure of microplate dismounting, and this container part has has only accommodated the portion that retains that utilizes the effective constituent that filtrator separates.
Patent documentation 1: Japanese Unexamined Patent Application Publication 2006-505278 communique
Patent documentation 2: Japanese kokai publication hei: 4-158779 communique
So far in general known known microplate is made of a plurality of key elements.Though these key elements need be assembled rapidly and with clean conditions, in these known microplates, mainly be the exploitation that is conceived to filtrator, and will assemble the key element that constitutes microplate very difficult rapidly and wholesomely.In addition, microplate generally uses the filtrator of extremely thin and easy destruction, therefore, in the moving of microplate, the change in location of this filtrator often takes place and causes damaged accident.And, when filtration is examined material,, require the mesh of filtrator to form very carefully sometimes, but in the case, exist to be examined material by filtrator needs problem for a long time according to the difference of the scope of examination.And, in microplate, needing in order to check a plurality of corpse or other object for laboratory examination and chemical testing under the situation of a plurality of microplates, stacked a plurality of microplates are also very difficult.
And, the sample that retains in the portion when check attracting may mix with the adjacent sample that retains in the portion, and, when when checking attraction, applying big negative pressure etc., sample may flow backwards, and also has when carrying out this attraction sample may flow out to the problem of the outside of the portion of retaining.
Summary of the invention
In order to address these problems, the present application provides a kind of microplate with filtrator, comprising: go up cubitainer, have and be used to inject the peristome that is examined material; Last side seal and following side seal are keeping filtrator; Link parts, be and the above-mentioned binding parts of going up the cubitainer tabling, have the peristome that the inspection sample that passed through filtrator is passed through, link parts with should between the cubitainer cramping keep side seal and following side seal; Lower reservoir is to have the lower reservoir of accommodating the portion that retains that checks sample, and this lower reservoir is to be maintained with respect to linking parts dismounting mode freely.
In addition, in order to address these problems, the present application also provides a kind of microplate with filtrator, comprising: go up cubitainer, possess and be used to inject the peristome that is examined material; Last side seal and following side seal are keeping filtrator; Intermediate receptacle, be and the above-mentioned intermediate receptacle of going up the cubitainer tabling, have the peristome that the inspection sample that passed through filtrator is passed through, intermediate receptacle with should on cramping maintenance seal between the cubitainer; Lower reservoir is to have the lower reservoir of accommodating the portion that retains that checks sample, and this lower reservoir is to be maintained with respect to intermediate receptacle dismounting mode freely; Intermediate receptacle has from peristome and hangs down and have the guiding wall of lower opening portion in the bottom; Above-mentioned guiding wall is accommodated by the portion of retaining that lower reservoir had, and this retains portion and accepts the inspection sample of supplying with via filtrator from the lower opening portion of guiding wall, and, have the ventilating part of the outside of leading to the portion of retaining on this lower reservoir hole enlargement inclined-plane above it.
The problems referred to above solve by the microplate that the band filtrator shown in following (1)～(23) is provided.
(1) provides a kind of microplate 110, comprising: go up cubitainer 111, have and be used to inject the peristome 120 that is examined material with filtrator; Last side seal 113 and following side seal 114 are keeping filtrator 115; Link parts 112, be and the above-mentioned binding parts 112 of going up cubitainer 111 tablings, have the peristome 140 that the inspection sample that passed through filtrator 115 is passed through, link parts 112 with should between the cubitainer 111 cramping keep side seal 113 and following side seal 114; Lower reservoir 116 is to have the lower reservoir 116 of accommodating the portion that retains 160 that checks sample, and this lower reservoir 116 is to be maintained with respect to linking parts 112 dismounting mode freely.
(2) provide a kind of microplate with filtrator, the microplate as (1) described band filtrator is characterized in that, last cubitainer 111 has the outer vertical wall 124 of extending to vertical lower at outer peripheral portion, and this outer vertical wall 124 has the outstanding projection 125 towards foreign side; Link parts 112 and have to what vertical direction was extended at outer peripheral portion and erect wall 135, this erects wall 135 and has the outstanding projection 136 in side inwardly; These projection 125,136 engagings, thus realize going up cubitainer and link the chimeric of parts.
(3) provide a kind of microplate with filtrator, microplate as (1) or (2) described band filtrator, it is characterized in that, last cubitainer 111 has the embedded hole 128 that is made of upper section 129 and below part 130, upper section has the hole enlargement hole, the below part has the undergauge hole, forms stage portion 131 up between part and the below part; Link the stop pin 143 that parts 112 have the hollow shape of extending towards the top, this stop pin has hole enlargement part 144 on top, and this hole enlargement partly has a plurality of slot parts that extend along axis direction; Be pressed into this stop pin 143 by below part 130 to embedded hole 128 from last cubitainer 111, the hole enlargement part 144 of this stop pin moves and undergauge to center position, by further being pressed into, stop pin 143 arrives upper section 129, this hole enlargement part 144 is engaged on the stage portion 131 of cubitainer 111, thus, cubitainer 111 and link the chimeric of parts 112 in the realization.
(4) provide a kind of microplate with filtrator, microplate as each described band filtrator in (1) to (3), it is characterized in that, link parts 112 and have maintenance wall portion 137 and the register pin 145 that extends towards the below, this maintenance wall portion 137 is made of outside surface 138 and inclined inner surface 139 and is configured in outer peripheral portion to surround the mode that links parts 112, register pin 145 has cone shape, and, be provided with a plurality of register pins 145 at the inside part that links parts 112; Lower reservoir 116 has by rake 162 and step-like 163 periphery flank 161 that constitutes and the register pin carrier 164 that is cone shape at outer peripheral portion; By making lower reservoir 116 join with airtight conditions and binding parts 112 with respect to inclined inner surface 139 crimping rake 162, it is loosening chimeric that register pin 145 and register pin carrier 164 separate set compartment of terrain.
(5) provide a kind of microplate with filtrator, microplate as each described band filtrator in (1) to (4), it is characterized in that, filtrator 115 is made by the etching and processing silicon chip, be made of the central part of the through hole with a plurality of size homogeneous and the peripheral part of this central part of encirclement, peripheral part forms thicklyer than central part.
(6) provide a kind of microplate with filtrator, microplate as each described band filtrator in (1) to (5), it is characterized in that, in the rake 162 of inclined inner surface 139 that links parts 112 and lower reservoir 116 one on be embedded with O shape ring etc. and can carry out airtight parts, thus, both are chimeric airtightly.
(7) provide a kind of microplate with filtrator, microplate as (4) described band filtrator, it is characterized in that, it is chimeric airtightly at separately inclined inner surface 139 and rake 162 places to link parts 112 and lower reservoir 116, in other parts then is loosening chimeric, by the mechanism of decompressor being connected to a register pin carrier 164, can make the below of filtrator become negative pressure, thus, can shorten filter time.
(8) provide a kind of microplate with filtrator, the microplate as each described band filtrator in (1) to (7) is characterized in that, last side seal 113 and following side seal 114 are the structure of one.
(9) provide a kind of microplate with filtrator, microplate as each described band filtrator in (1) to (8), it is characterized in that, on the parts of filtrator 115 or this filtrator institute butt, be applied with the mark that is used for the particular group holding position, can carry out both accurate location thus, thereby can realize the robotization of assembling operation, and then a large amount of production also becomes easy.
(10) provide a kind of microplate 40,40A, comprising: go up cubitainer 41,41A, possess and be used to inject peristome 50, the 50A that is examined material with filtrator; Last side seal 44,44A and following side seal 45,45A are keeping filtrator 46,46A; Intermediate receptacle 42,42A, be and above-mentioned cubitainer 41, the intermediate receptacle 42 of 41A tabling, the 42A of going up, have the peristome 63 that the inspection sample that passed through filtrator 46,46A is passed through, intermediate receptacle 42,42A and should between the cubitainer 41,41A cramping keep side seal 44,44A and following side seal 45,45A; Lower reservoir 43,43A are to have to accommodate the portion that retains 80, the lower reservoir 43 of 80A, the 43A that checks sample, and this lower reservoir 43,43A are to be maintained with respect to intermediate receptacle 42,42A dismounting mode freely; Intermediate receptacle 42,42A have from peristome 63 and hang down and have guiding wall 64, the 64A of lower opening portion 65 in the bottom; Lower reservoir 43, the portion that retains 80,80A that 43A had accommodate this guiding wall 64,64A, this retains portion 80,80A and accepts the inspection sample of supplying with via filtrator 46,46A from the lower opening portion 65 of guiding wall 64,64A, and, have the ventilating part 90 of the outside of leading to the portion of retaining 80,80A on this lower reservoir 43, the 43A hole enlargement inclined-plane 92 above it.
(11) provide a kind of microplate with filtrator, the microplate as (10) described band filtrator is characterized in that, is provided with a plurality of ventilating parts 90.
(12) provide a kind of microplate with filtrator, the microplate as (11) described band filtrator is characterized in that, is provided with two ventilating parts 90.
(13) provide a kind of microplate with filtrator, the microplate as each described band filtrator in (10) to (12) is characterized in that the bottom of guiding wall 64,64A hangs down into the degree of depth over half at least of the portion of retaining 80,80A.
(14) provide a kind of microplate with filtrator, the microplate as each described band filtrator in (10) to (13) is characterized in that, between intermediate receptacle 42,42A and lower reservoir 43,43A auxiliary sealing member 47,47A is installed.
(15) provide a kind of microplate with filtrator, the microplate as (14) described band filtrator is characterized in that, auxiliary sealing member 47,47A are shaped by unlike material in advance or embedded shaping is bonded on lower reservoir 43, the 43A.
(16) provide a kind of microplate with filtrator, microplate as each described band filtrator in (10) to (15), it is characterized in that, last cubitainer 41,41A have outer vertical wall 52, the 52A that extends to vertical lower at peripheral part, and this outer vertical wall 52,52A have the outstanding projection 53 towards foreign side; Intermediate receptacle 42,42A have to what vertical direction was extended at peripheral part and erect wall 60,60A, and this erects wall 60,60A has the outstanding projection 61 in side inwardly; These projection 53,61 engagings, thus realize going up the chimeric of cubitainer 41,41A and intermediate receptacle 42,42A.
(17) provide a kind of microplate with filtrator, microplate as each described band filtrator in (10) to (16), it is characterized in that, last cubitainer 41,41A have the embedded hole 56 that is made of upper section 57 and below part 58, upper section 57 has the hole enlargement hole, below part 58 constitute along with from the below towards the top and the hole of undergauge forms stage portion 59 up between part 57 and the below part 58; Intermediate receptacle 42,42A have the stop pin 67 that extends towards the top, and this stop pin 67 has hole enlargement part 69 on top, and this hole enlargement part 69 has the interval 48 of extending to axis direction; Be pressed into this stop pin 67 by below part 58 to embedded hole 56 from last cubitainer 41,41A, the hole enlargement part 69 of this stop pin 67 moves and undergauge to center position, by further being pressed into, stop pin 67 arrives upper section 57, this hole enlargement part 69 is engaged on the stage portion 59 of cubitainer 41,41A, thus, cubitainer 41,41A and intermediate receptacle 42,42A chimeric in the realization.
(18) provide a kind of microplate with filtrator, microplate as each described band filtrator in (10) to (17), it is characterized in that, filtrator 46,46A make by the etching and processing silicon chip, constitute by the central part of the through hole with a plurality of size homogeneous with from the peripheral part of this central part via this central part of encirclement of rake extension, peripheral part forms thicklyer than central part.
(19) provide a kind of microplate with filtrator, the microplate as each described band filtrator in (10) to (18) is characterized in that each inscape is made of transparent material.
(20) provide a kind of microplate with filtrator, microplate as each described band filtrator in (10) to (19), it is characterized in that, last cubitainer 41A has flange rising portions 86 that extends upward from peripheral part and the opening rising portions 87 that extends upward continuously from peristome 50A, and these flange rising portions 86 and opening rising portions 87 extend to substantially the same height.
(21) provide a kind of microplate with filtrator, microplate as each described band filtrator in (10) to (20), it is characterized in that, intermediate receptacle 42A has from what peripheral part extended upward and erects wall 60A and separate the inboard of both inboards of set a distance extending upward and erect wall 88 from erecting wall 60A with this, these erect wall 60A and inboard erect embed in the space that limits between the wall 88 from the outer vertical wall 52A that hangs down of cubitainer 41A, the height that the inboard erects wall 88 is lower than the height that erects wall 60A.
(22) provide a kind of microplate with filtrator, the microplate as (21) described band filtrator is characterized in that, the inboard erects the outer peripheral edges that wall 88 keeps going up side seal 44A and following side seal 45A.
(23) provide a kind of microplate with filtrator, the microplate as each described band filtrator in (14) to (22) is characterized in that, intermediate receptacle 42A has protuberance 89 on the face that joins with auxiliary sealing member 47A.
Microplate according to the band filtrator of above-mentioned (1) of the present application record, a kind of microplate can be provided, it can support safely by very simple key element and constitute and filtrator that can promptly assemble, health and destructible, and, can prevent its change in location effectively.
In addition, the microplate according to the band filtrator of above-mentioned (2) of the present application record can provide a kind of microplate, and it is assembled easily and takes out inspection sample after filtering from microplate easily, therefore, can solve the problem that these operations need proficiency.In addition, according to the microplate of band filtrator of above-mentioned (3) record, can provide a kind of can be easily and correctly link the microplate of the binding of parts and last cubitainer.
In addition, the microplate according to the band filtrator of above-mentioned (4) of the present application record can provide a kind of microplate, and it can be configured with stable status to rest position.And the microplate of band filtrator according to above-mentioned (5) record can provide a kind of microplate, and it can be provided with under the situation of the filtrator that does not damage crisp fritter, and can always filtrator be remained on the appropriate location.
Microplate according to the band filtrator of above-mentioned (6), (7) record can provide a kind of microplate, and it can shorten filtration time.And the microplate according to the band filtrator of above-mentioned (8), (9) record can provide a kind of microplate, and its constituent part is few, and assembling operation is easy, can produce in a large number.
And, microplate according to the band filtrator of above-mentioned (10) of the present application record can provide a kind of microplate, and it can support safely by very simple key element and constitute and filtrator that can promptly assemble, health and destructible, and, can prevent its change in location effectively.In addition, ventilating part is being set, can forcibly sample be attracted in the portion of retaining, therefore, can expect that the decompression of not having waste attracts effect by this ventilating part with the portion position adjacent of retaining.As a result, can be rapidly and carry out filtration operation effectively, can shorten the cultivation at initial stage or check time of operation.
And the microplate according to the band filtrator of above-mentioned (11) of the present application, (12) record because a plurality of ventilating parts are set, can carry out filtration operation with the time that is suitable for most sample thereby can be adjusted to.As a result, sample bottom line can be restricted to because of contact the problem that changes with air, thus, very correct analysis result can be expected to obtain.In addition, the microplate of band filtrator according to above-mentioned (13) record, the bottom of guiding wall is that lower opening portion extends to than half of the portion of retaining more by the position of below, therefore, can prevent to disperse to beyond thought space from the sample that this lower opening portion supplies with, as a result, all samples that passed through filtrator all can be used for analyzing etc., thereby can realize high efficiency operation.
In addition, according to the microplate of the band filtrator of above-mentioned (14) of the present application, (15) record, auxiliary sealing member is configured between intermediate receptacle and the lower reservoir.As a result, the ventilating part of the upper section by being arranged on the portion of retaining can guarantee that air always flows.In addition, this auxiliary sealing member can utilize be very easy to and reliably mechanism both be configured on the allocation.In addition, according to the microplate of the band filtrator of above-mentioned (16) of the present application, (17) record, only by push operation can be simply at short notice reliably assembling go up cubitainer and intermediate receptacle.According to the microplate of band filtrator of above-mentioned (18) record, the filtrator of the structure with the filtrator ground setting that can not damage crisp fritter can be provided, the result can be with filter deployment in position always.The microplate of band filtrator according to above-mentioned (19) record, because the microplate of band filtrator is formed by transparent material, so, those skilled in the art can stop sample supply effectively before the lower opening portion that waits to accommodate the sample arrival guiding wall in the portion of retaining, the result, can always remove the air that retains portion inside, can prevent that sample from attracteding to reliever inside.
In addition, microplate according to the band filtrator of above-mentioned (20) of the present application record, flange rising portions 86 that extends upward from the peripheral part of last cubitainer 41 and the opening rising portions 87 that extends upward continuously from peristome 50A extend to substantially the same height, therefore, provide the peristome of sample to enlarge, the height of microplate integral body increases, and is easy thereby its operation becomes.In addition, according to the microplate of the band filtrator of above-mentioned (20), (21) record, the inboard erect wall 88 with erecting that wall 60A cooperate cubitainer 41A in the powerful chimeric maintenance, and the inboard erects wall 88 and keeps side seal 44A and following side seal 45A.Thus, can provide constitutionally stable firm microplate.
And, microplate according to the band filtrator of above-mentioned (23) of the present application record, sample is attracted to retain among the 80A of portion after, be arranged on lower reservoir 43A always is attached to protuberance 89 on the lower reservoir 43A from the middle auxiliary sealing member 47A that makes when container 42A unloads, thus, can prevent that auxiliary sealing member 47 from unexpectedly polluting the danger that retains the sample in the 80A of portion.
Description of drawings
Fig. 1 is the vertical view of microplate of band filtrator of the embodiment 1 of the present application.
Fig. 2 is the A-A sectional view of Fig. 1.
Fig. 3 is the B-B sectional view of Fig. 1.
Fig. 4 is the enlarged drawing of the X part of Fig. 2.
Fig. 5 is the C-C sectional view of Fig. 1.
Fig. 6 is the enlarged drawing of the Y part of Fig. 3.
Fig. 7 is the vertical view of microplate of band filtrator of the embodiment 2 of the present application.
Fig. 8 is the 2-2 sectional view of Fig. 7, is the figure of expression use location.
Fig. 9 is the enlarged drawing with circle 3 parts of representing of Fig. 8.
Figure 10 is the 4-4 sectional view of Fig. 7.
Figure 11 is the enlarged drawing with circle 5 parts of representing of Figure 10.
Figure 12 is the 6-6 profile of Fig. 7.
Figure 13 is the vertical view of last cubitainer of microplate that constitutes the band filtrator of the present application.
Figure 14 is the 8-8 sectional view of Figure 13.
Figure 15 is the enlarged drawing with circle 9 parts of representing of Figure 14.
Figure 16 is the 10-10 sectional view of Figure 13.
Figure 17 is the enlarged drawing with circle 11 parts of representing of Figure 16.
Figure 18 is the enlarged drawing with circle 12 parts of representing of Figure 16.
Figure 19 is the vertical view of intermediate receptacle that constitutes the microplate of band filtrator of the present invention.
Figure 20 is the rear view of intermediate receptacle shown in Figure 19.
Figure 21 is the side view that 15-15 saw from Figure 19.
Figure 22 is the 16-16 sectional view of Figure 19.
Figure 23 is the enlarged drawing with circle 17 parts of representing of Figure 22.
Figure 24 A is enlarged drawing, the sectional view of stop pin shown in Figure 22.
Figure 24 B is enlarged drawing, the vertical view of stop pin shown in Figure 22.
Figure 25 is the 19-19 sectional view of Figure 19.
Figure 26 is the vertical view of lower reservoir of microplate that constitutes the band filtrator of the present application.
Figure 27 is the rear view of lower reservoir shown in Figure 26.
Figure 28 is the side view that 22-22 saw from Figure 26.
Figure 29 is the 23-23 sectional view of Figure 26.
Figure 30 is the enlarged drawing with circle 24 parts of representing of Figure 29.
Figure 31 is the 25-25 profile of Figure 26.
Figure 32 is the 26-26 profile of Figure 26.
Figure 33 is the vertical view of last side seal that constitutes the microplate of band filtrator of the present invention.
Figure 34 is the rear view of last side seal shown in Figure 33.
Figure 35 is the 29-29 sectional view of Figure 33.
Figure 36 is the enlarged drawing with circle 30 parts of representing of Figure 35.
Figure 37 is the profile with lead-in wire 31 parts of representing of Figure 33 and Figure 34.
Figure 38 is the vertical view of following side seal that constitutes the microplate of band filtrator of the present invention.
Figure 39 is the vertical view of auxiliary sealing member of microplate that constitutes the band filtrator of the present application.
Figure 40 A is the (a～e) of vertical view separately of five different embodiment of microplate that the band filtrator of the present application is shown enlargedly.
Figure 40 B is the sectional view (A～E) of five different embodiment of microplate that the band filtrator of the present application is shown enlargedly.
Figure 41 is the figure same with Fig. 9 of the embodiment 3 of expression the present application.
Figure 42 is the figure of bottom container that the present application, expression have the mark of the position that is used to specify each peristome.
Figure 43 is the lower reservoir flank of expression the present application and the figure of lower reservoir guide portion.
Description of reference numerals
30 lower reservoir ribs; 35 lower reservoir guide portion; 41, the last cubitainer of 41A; 42,42A intermediate receptacle; 43,43A lower reservoir; 44, the last side seal of 44A; 45, side seal under the 45A; 46,46A filtrator; 47,47A auxiliary sealing member; 48 at interval; 49 rakes; 50,50A peristome; 51,51A cone shape wall; 52,52A outer vertical wall; 53 projections; 54,54A flange; 55 contact rings; 56 embedded holes; 57 upper sections; 58 below parts; 59 stage portion; 60,60A erects wall; 61 projections; 62,62A keeps wall portion; 63 peristomes; 64,64A guiding wall; 65 lower opening portions; 66 contact rings; 67 stop pins; 68 erect sheet; 69 hole enlargement parts; 70,70A contact ring; 71 peristomes; 72 stage portion; 73 openings; 74 recesses; 75 peristomes; 76 openings; 77 protrusions; 80,80A retains portion; 81 external circumferential ribs; 82 first portions that hang down; 83 second portions that hang down; 84 the 3rd portions that hang down; 85 stacked flanks; 86 flange rising portions; 87 opening rising portions; 88 inboards erect wall; 89 protuberances; 90 ventilating parts; 91 upper surfaces; 92 hole enlargement inclined-planes; 93 curves; 94 peristomes; 95 curves; 96 circumscribed circles; 97 circumscribed circles; 98 retain the length-wise axis of portion; The length-wise axis of 99 guiding walls; L1～L7 size; The microplate of 110 band filtrators; Cubitainer on 111; 112 link parts; Side seal on 113; 114 times side seals; 115 filtrators; 116 lower reservoirs; 120 peristomes; 121 both sizings; 122 cone shape walls; 123 both sizings; 124 outer vertical wall; 125 projections; 126 flanges; 127 contact rings; 128 embedded holes; 129 upper sections; 130 below parts; 131 stage portion; 135 erect wall; 136 projections; 137 keep wall portion; 138 outside surfaces; 139 inclined inner surfaces; 140 peristomes; 141 guiding walls; 142 contact rings; 143 stop pins; 144 hole enlargement parts; 145 register pins; 146 pedestals; 150 peristomes; 151 stage portion; 155 peristomes; 160 retain portion; 161 external circumferential ribs; 162 rakes; 163 step-like portions; 164 register pin carriers; 166 stationary faces; 170 at interval; 171 paths.
Embodiment
Below, the preferred forms that is used to implement the present application is described.
Embodiment
[embodiment 1]
Fig. 1 is the vertical view of microplate 110 of band filtrator of an embodiment that amplifies the device of expression the present application.As shown in the figure, the surface of this microplate has rectangular shape, and integral body has for example long limit (120～150mm) * minor face (80～100mm) * thickness (rectangular parallelepiped about 10～20mm).But for a person skilled in the art, this size and dimension obviously can change according to purposes and other requirements.Therefore, the surface configuration of the microplate 110 of the band filtrator of the present application for example, also can be circle or oval except illustrated oblong-shaped and other rectangular shapes.But, in the following embodiments, the microplate of above-mentioned rectangular shape is described.This microplate 110 is to dispose a plurality of (be 12 * 8 in the example at Fig. 1, amount to 96) peristome 120 on whole of upside in face side, by these peristomes 120 nutrient solution (for example the blood of Cai Jiing etc. be examined material) is supplied in the microplate.
As Fig. 2～shown in Figure 5, the structure of the microplate 110 of the present application has: the last cubitainer 111 that is formed with above-mentioned peristome 120; Chimeric and be fastened on binding parts 112 on this container 111; Be clamped in last side seal 113 and the following side seal 114 gone up between cubitainer 111 and the binding parts 112; Be configured in the filtrator 115 of both allocations of the last side seal 113 that joins with last cubitainer 111; And the lower reservoir 116 that is configured in the downside of above-mentioned binding parts 112.
From Fig. 2～Fig. 5 as can be known, last cubitainer 111 and link parts 112 and have roughly the same area, only lower reservoir 116 has the area that is a bit larger tham cubitainer 111 and binding parts 112 these on.In addition, seal 113,114 disposes in the mode that is enclosed in the cubitainer 111.And, be configured in the area that side seal 113 interior filtrators 115 have the area that is a bit larger tham above-mentioned peristome 120, in the example of Fig. 2～Fig. 5, disposed 96 filtrators 115 altogether at the downside of peristome 120 correspondingly with peristome 120.At this, last cubitainer 111, link parts 112 and lower reservoir 116 generally by have stability characteristic (quality) that chemically-resistant changes, the plastic material of elastic deformation (for example polyvinyl resin) forms slightly.In addition, seal 113,114 is formed by the soft material (for example silicone) of the stability characteristic (quality) with chemically-resistant variation equally.Filtrator 115 for example forms by the etching silicon chip.
In following embodiment, in order to understand structure easily, the part that seal 113,114 is divided into two splits is put down in writing, but, be not limited thereto, for a person skilled in the art, keep form by changing filtrator, thereby these seals are integrally formed and form monomer and realize the minimizing of number of spare parts and the facilitation of assembling operation, and this is easy to obtain.
As Fig. 1～shown in Figure 4, on whole of last cubitainer 111, roughly dispose peristome 120 equably with set circle of the same area.The cone shape wall 122 of sizing 121 forms these peristomes 120 by both sinking with respect to vertical direction from the surface of last cubitainer 111 integratedly towards downside.And as shown in Figure 4, whole outside on cubitainer 111 surfaces on this is both generally perpendicularly extended the outer vertical wall 124 of sizing 123 and is sunk integratedly and form towards the below from this surface.At this, both sizings 123 of outer vertical wall 124 are bigger slightly than the size 121 of cone shape wall 122.Utilize this difference in size, outer vertical wall 124 is enclosed in above-mentioned seal 113,114 in the zone that is limited by this vertical wall 124.In addition, on the lateral surface of this outer vertical wall 124, be formed with outside projection 125 with circular section.This projection 125 is preferably formed on the lateral surface complete cycle of this outer vertical wall 124, still, also is not limited thereto.
In addition, the outside of the outer vertical wall 124 of cubitainer 111 is formed with flange 126 towards radial direction foreign side on this, and this flange 126 has the function of protection binding parts 112 described later.And, on the face that in the lower surface portion of above-mentioned each cone shape wall 122, joins, form a plurality of (being 2 in the example of Fig. 4) roughly contact ring 127 of concentric circles with last side seal 113.These rings 127 are pushed seal 113 and are played the function that prevents sealing part generation change in location.
As Fig. 1, Fig. 3 and shown in Figure 6, on the container 111, form a plurality of embedded holes 128 in the zone between peristome 120 up.In example shown in Figure 1, this embedded hole 128 from the right side vertically on, the 2nd row and the 3rd row between, the 4th row and the 5th row between, the 6th row and the 7th row between, the 8th row and the 9th row between and the 10th row and the 11st row between, from on transversely, the 2nd row and the 3rd row between, the 4th row and the 5th row between, the 6th row and the 7th row between the zone in, be provided with 15 altogether, but be not limited thereto.Also can be provided with more or lessly.
As shown in Figure 6, this embedded hole 128 is formed by the hole of the circular section that hangs down with certain diameter from the surface of last cubitainer 111.This hole is slightly smaller than the diameter of above-mentioned peristome 120 by diameter upper section 129 and the diameter below part 130 littler than this upper section constitute.Form stage portion 131 up between part 129 and the below part 130.
As shown in Figure 4, on this surperficial outer peripheral edges of cubitainer 111 and peristome 120 around be formed for the small protrusion strengthened respectively.This projection need not to be arranged on the below part of all embedded holes 128, for example from the right side vertically on, between the 4th row and the 5th row, between the 8th row and the 9th row, from above transversely, be provided with 4 altogether and get final product in the zone between the 2nd row and the 3rd row, between the 6th row and the 7th row.But, be not limited thereto, also can be provided with more or lessly.
The projection 125 of the outer vertical wall 124 of this projection 136 cubitainer 111 on being arranged at and should on be formed on the position that engages with this projection 125 between the flange 126 of cubitainer 111.Thus, the projection 136 that has the projection 125 by making cubitainer 111 and link parts 112 is carried out the chimeric incorporate function that realizes both.This projection 136 is preferably formed on this medial surface complete cycle that erects wall 135, but is not limited thereto.That is to say, also can be arranged on as on the set position of the projection 125 of the last cubitainer 111 of chimeric object.Thus, carry out chimeric operation easily, can realize suitableization of materials used.This erects wall 135 and extends to than the position of this projection 136 more by the top and the degree of not joining with the flange 126 of last cubitainer 111.Thus, strengthen this and erect wall 135 and the stable chimerism of going up cubitainer 111 and linking parts 112.
And, be provided with integratedly near the periphery of parts 112 to the direction opposite and from this maintenance wall portion 137 that hangs down downwards linking with erecting wall 135.This maintenance wall portion 137 preferably hangs down in the mode of the outside integral body of surrounding these binding parts 112.This maintenance wall portion 137 preferably constitutes by the outside surface 138 that roughly hangs down from binding parts 112 at right angles with from the square inwardly oblique inclined inner surface that erects 139 in the bottom of outside surface, and has general triangular cross section.
In addition, linking on the parts 112, when will linking parts 112 and being assembled on the cubitainer 111 be formed at this on the corresponding position of the peristome 120 of cubitainer 111, form the peristome 140 of the circular section identical with the number (being 96 in the example of Fig. 4) of this peristome 120.As shown in Figure 4, the diameter of this peristome 140 is slightly smaller than the diameter of peristome 120.This peristome 140 is made of thin petal-like guiding wall 141, and this guiding wall 141 hangs down towards the center position below of this peristome 140 integratedly from linking parts 112.The below terminal part of the guiding wall 141 that hangs down towards the center position below of this peristome 140 forms circular open portion 140.The terminal part of this guiding wall 141 extends towards the direction of the portion that retains 160 of lower reservoir 116 described later, has the function that the subject things such as nutrient solution that will feed to the peristome 120 of cubitainer 111 are directed to the portion of retaining 160 effectively.
And, in linking the upper surface of parts 112, this forms the periphery of the part of guiding wall 141, to be formed with a plurality of (being 2 in the example of Fig. 4) contact ring 142 for concentrically ringed mode roughly with this guiding wall 141.These contact rings 142 contact, push sealing part 114 with following side seal 114 and play the function that prevents sealing part generation change in location.
And, as Fig. 1, Fig. 3 and shown in Figure 6, linking on the parts 112, with above-mentioned on the embedded hole 128 corresponding positions of cubitainer 111, a plurality of (being 15 in these illustrated embodiment) stop pin 143 erects and is formed on this position from the upper surface of these binding parts 112.These stop pins 143 preferably have hollow shape.The top of stop pin 143 is the hole enlargement part 144 with hole enlargement size, and the top of this hole enlargement part 144 has the roughly such shape of abacus pearl.And, to the part that erects of hollow shape, for example the slot part with set width dimensions longitudinally is set from the top of this abacus pearl shape every 90 degree.Thus, producing on the top of this abacus pearl shape can be to the flexible elastic property of radial direction.
The angle that slot part is set also can be every 60 or 120 degree or angle in addition.Mainly be to wish can not produce the deflection of elastic property by slot part being set every equal angles.The length of stop pin 143 for when being assembled into this pin on the cubitainer 111 not from this on the outstanding degree in surface of cubitainer 111.This is in order to obtain stable stacked state when the microplate of stacked a plurality of the present application.In the embodiment shown in fig. 6, thereby stop pin 143 carries out chimeric can't the separation with respect to embedded hole 128 with so-called fixed installation state, still, finds that in for example assembling back filtrator goes wrong when waiting, and needing should bad filtrator taking-up and change.Therefore, also can suitably regulate the width dimensions of the vertical slot part that is formed at above-mentioned hole enlargement part 144, so that can as required stop pin 143 be separated from embedded hole 128.
In addition, on the position corresponding to stop pin 143 positions that links parts 112, a plurality of (being 15 in the example of Fig. 6) register pin 145 preferably forms from the lower surface one of these binding parts 112 with hanging down.This register pin 145 has more to next thin more conical shaped shape.These register pins 145 have the function that the installation site to lower reservoir 116 described later positions.And on the concentric circles of this register pin 145, pedestal 146 preferably forms with the binding parts.This pedestal 146 has as linking the function that limits the distance member of set interval usefulness between parts 112 and the lower reservoir 116.
On last side seal 113, with above-mentioned on form a plurality of (in the example at Fig. 1 being 96) circular peristome 150 respectively on peristome 120 corresponding each position of cubitainer 111.On the downside of the sealing part 113 of the periphery of these peristomes 150, be provided with stage portion 151.The shape of this stage portion 151 is consistent with the shape and the gauge of filtrator 115 with the degree of depth.The diameter of peristome 150 is roughly the same with the diameter that the bottom limited that is formed at the above-mentioned upward cone shape wall 122 of cubitainer 111.
On following side seal 114, with above-mentioned on similarly form a plurality of (in the example at Fig. 1 being 96) circular peristome 155 respectively on peristome 120 corresponding each position of cubitainer 111.The diameter of preferred these peristomes 155 is also roughly the same with the diameter that the bottom limited that is formed at the above-mentioned upward cone shape wall 122 of cubitainer 111.Therefore, following side seal 114 has the size and dimension roughly the same with last side seal 113, but stage portion such on the side seal 113 151 is not set.In addition, this time side seal 114 has the thickness roughly the same with last side seal 113.In the example of Fig. 5, consider the operation when assembling is used to keep the stage portion 151 of filtrator 115 and stage portion 151 is arranged on side seal 113, still, also this stage portion 151 can be arranged on down on the side seal 114.And, also the stage portion with half gauge of filtrator 115 peripheral parts can be arranged on respectively on side seal and the following side seal.
On lower reservoir 116, with above-mentioned on be formed with the portion that retains 160 (with reference to Fig. 4 and Fig. 5) that a plurality of (in the example at Fig. 1 being 96) generally perpendicularly hang down from the surface of this lower reservoir 116 on the peristome 120 corresponding positions of cubitainer 111.This retains portion 160 and has following function, promptly after utilizing 115 pairs of filtrators to supply to being examined material and filtering of peristome 120 of cubitainer 111, only receives and accommodate the inspection sample after this filtration.Therefore, this retains portion 160 size for the volume that can guarantee to accommodate necessary inspection sample.
And, form at the peripheral part of this lower reservoir 116 and to retain the periphery flank 161 that portion 160 extends downwards than this.The rake 162 of side-lower and step-like 163 of extending with the downwards step-like and outside roughly from the bottom of this rake 162 constituted outside this periphery flank 161 was extended to by the surface from lower reservoir 116.These periphery flanks 161 have when the microplate 110 of stacked a plurality of the present application can carry out stacked function with stable status.Therefore, the bottom of this periphery flank 161 ratio retains portion 160 more by the below.And rake 162 has basically with tight contact condition and touches angle of inclination on the inclined inner surface 139 that links parts 112.Thus, lower reservoir 116 and binding parts 112 can engage with tight state of contact.
In addition, as shown in Figure 6, on this lower reservoir 116, preferably form a plurality of (being 15 in illustrated embodiment) register pin carrier 164.This register pin carrier 164 has cone shape, and its inside surface is accepted the register pin 145 of above-mentioned binding parts 112 across both fixed gaps.The bottom of this register pin carrier 164 is an open state.
When the microplate of band filtrator of assembling the present application, at first, the state that last cubitainer 111 is configured to overturn.Then, last side seal 113 is arranged on the last cubitainer 111 of this rollover states.At this moment, the stage portion 151 that should be noted in the discussion above that side seal 113 is provided with in the mode towards the top.Then, filtrator 115 is arranged on the stage portion 151 of side seal 113 on this.At this moment, filtrator 115 is with state (rollover states) configuration opposite with when work.After this, following side seal 114 is configured in the upper surface of side seal 113 and filtrator 115 on this.Be formed in the stage portion that is being used to keep filtrator 115 under the situation of side seal down, on being provided with after the side seal, be positioned on this on side seal in advance filtrator 115 being arranged on following side seal on this stage portion.At this, last side seal 113 and following side seal 114 all suitably are configured in the inboard of the outer vertical wall 124 of cubitainer 111.
Then, the upper surface that parts 112 are arranged on down side seal 114 will be linked.At this moment, these binding parts 112 are located in the mode that erects side seal 114 under the wall 135 downward also coverings with projection 136, container 111 sides push that to link parts 112 chimeric fully with the projection 125 on the outer vertical wall 124 that is arranged on cubitainer 111 until the projection 136 of these bindings parts 112 upward, and the periphery area of affirmation binding parts 112 and last cubitainer 111 is chimeric effectively then.Then, insert the embedded hole 128 of going up cubitainer 111 with being arranged at 115 stop pins 143 that link parts 112.The diameter dimension of stop pin 143 is a bit larger tham the diameter dimension of the below part 130 of embedded hole 128.But, by stop pin 143 forcibly being pressed into this below part 130, the top with abacus pearl shape that makes this stop pin 143 relies on center position, and thereby undergauge takes place because of the existence of groove, and therefore, stop pin 143 is embedded in this below part 130 easily.
If stop pin 143 further is pressed in the embedded hole 128, then stop pin 143 arrives the upper section 130 with hole enlargement size.Therefore, return to common diameter state for the top of the stop pin 143 of undergauge state before this.Therefore, this stop pin 143 is supported by the stage portion 131 of embedded hole 128 and stops stop pin to be deviate from from embedded hole.So, need to confirm that stop pin 143 is fitted in the stage portion 131 effectively.This can easily determine along axis direction moves up and down, stop pin sends when being embedded on the stage portion sound by making stop pin.
In the moment after projection 136 on the wall 135 and stop pin 143 are assembled into the appropriate location of erecting that will be set in place, the assembling that finish cubitainer 111, links parts 112, goes up side seal 113, time side seal 114 and filtrator 115 in the neighboring that links parts 112.Under this state, the contact ring 127 of lower surface that is arranged on the cone shape wall 122 of cubitainer 111 closely contacts with last side seal 113, and the contact ring 142 that is arranged on the peristome periphery that links parts 112 closely contacts with following side seal 114.And these seals 113,114 constitute and crimping mutually by soft materials such as silicone.Therefore, can stop the offset of filtrator 115 fully.In addition, last cubitainer 111 and link parts 112 and utilize undercutting (the ア Application ダ-カ Star ト) pawl chimeric and embedded hole 128 and stop pin 143 that is configured in the projection 125,136 on the complete cycle respectively chimeric and realize practical closely chimeric.
At last, lower reservoir 116 is installed.With respect under rollover states, keep wall portion 137 binding parts 112 up, lower reservoir 116 is similarly carried thereon with rollover states.At this moment, the rake 162 of lower reservoir 116 disposes in the mode that closely touches on the inclined inner surface 139 that links parts 112.According to the angle that is shaped and the material of use etc., sometimes also need to consider on the rake 162 of inclined inner surface 139 that links parts 112 or lower reservoir 116, to form O shape annular groove etc., and O shape ring etc. is set in O shape annular groove can carries out airtight parts, then, flow out in order to stop air to pass through this part, lower reservoir 116 is set.
Next, respectively locating carrier 164 location and being pressed into the position of each stop pin 143 that links parts 112 lower reservoir 116.The interval 170 that the register pin carrier 164 of lower reservoir 116 separates narrow cone shape is configured in the periphery of the register pin 145 that links parts 112.At this moment, the portion that retains 160 of lower reservoir 116 disposes in the mode that the guiding wall 141 formed peristomes 140 with binding parts 112 are complementary.Under this state, as Fig. 4 and shown in Figure 6, between lower reservoir 116 and binding parts 112, form small gap, this gap forms path 171.This shows, link that wiping that parts 112 and lower reservoir 116 utilize the inclined inner surface 139 that is arranged on periphery separately and rake 162 is closed or wait and chimeric with airtight conditions by O shape ring, in the dip plane in addition then be to limit small interval 170 and path 171 between the two.And, in order to keep this path 171, linking formation pedestal 146 on the parts 112, these pedestals 146 contact with lower reservoir 116 and prevent that path 171 from collapsing under pressure.As mentioned above, finish the assembling of the microplate 110 of band filtrator.
The microplate of finishing assembling turns back to normal position shown in Figure 2, stacked in order keeping.Carrying out under the stacked situation, be arranged at flange 126 on the last cubitainer 111 of below microplate be arranged on above on the stacked flank 85 of step-like 163 inboard of microplate, thereby keep microplate effectively.Therefore, can be in order with stacked each microplate of stable posture.
Microplate for can in a large number production the present application applies telltale mark on each part, so that assembly mechanical can and carry out carrying out reference behind the correct location of each part with reference to the position of each part.Thus, can realize the robotization of assembling operation, can produce in a large number.Especially, in the application's microplate, because flimsy filtrator 115 is important factors in assembling operation with respect to the positioning operation of last cubitainer 111, so on the bight of filtrator 115 self or accept the mark that applies the particular group holding position on the parts (for example bight of seal) of this filtrator, can carry out the suitable location of this filtrator 115, this point is very important.
Next, the using method to the microplate 110 of the band filtrator of the embodiment 1 of the present application describes.As shown in Figure 2, the microplate 110 with a band filtrator is configured on both allocations of level.Then, utilize suitable utensils such as suction pipe will be examined the peristome 120 that material supplies to cubitainer 111.In the microplate 110 herein, can be that 96 corpse or other object for laboratory examination and chemical testing are gathered as the inspection sample simultaneously with the number of peristome 120.Fig. 4 below with reference to the section that amplifies expression peristome 120 describes, supply in the peristome 120 that the cone shape wall 122 by last cubitainer 111 limits be examined that material drips to that stage portion 151 by last side seal 113 is kept and the thin filtering surface by the central authorities of the filtrator 115 that descends side seal 114 clampings on.At this, can only make inspection sample by this filtrator 115 with set characteristic.The guiding wall 141 that garbled like this inspection sample is bonded parts 112 guides and accommodates in the portion that retains 160 of lower reservoir 116.
After having accommodated a certain amount of inspection sample, only the lower reservoir 116 with microplate 110 separates quietly from linking parts 112.This detached job can be by catching lower reservoir 116 periphery flank 161 and the maintenance wall portion 137 that links parts 112 easily carry out to being pulled away from towards upper and lower.Noting not making the portion that the retains 160 interior inspection samples of the lower reservoir that is housed in such separation to overflow on one side, will check that sample be transported to the set inspecting stand of subsequent processing, herein begin the detailed inspection of sample on one side.
Because the mesh size of the filtrator 115 that uses in the present application, may cause checked property to be blamed in by filtrator 115, be examined the problem that material generation rerum natura changes during this time.For this reason, in the microplate 110 of the present application, in order promptly to carry out filtration operation enough as far as possible with respect to checked property mass-energy, the portion that retains 160 to lower reservoir 116 of being provided with provides negative pressure, make and be examined the mechanism that material forcibly moves by filtrator, so that be housed in checked property mass-energy in the peristome 120 enough promptly by filtrator 115.
That is to say that the bottom of register pin carrier 164 of lower reservoir 116 of keeping the microplate of horizontality is an open state, connect the mechanism of decompressor with airtight conditions to the not shown a plurality of attraction portion that is communicated with it.Like this, start this mechanism of decompressor and begin decompression.As a result, the air in the portion that retains 160 of filtrator 115 belows is discharged from by being limited to the path 171, the interval 170 that link between parts 112 and the lower reservoir 116.The pressure that retains portion 160 becomes negative pressure, be examined material by filtrator 115 being forced to property be attracted to the inboard of the portion of retaining 160.Therefore, the set material that is examined in the material is promptly accommodated in the portion of retaining 160 by filtrator 115.At this moment, be inhaled in the above-mentioned path, on binding parts 112, guiding wall 141 be set in order to stop the inspection sample that has passed through filtrator 115.This guiding wall 141 when the inspection sample guiding that will pass through filtrator 115 retains portion 160, also played weaken air flow to the influence of this inspection sample so that this inspection sample can not be inhaled into the effect in the path.
[embodiment 2]
Fig. 7 is the vertical view of microplate 40 of the band filtrator of the present application.As shown in the figure, the surface of this microplate 40 has rectangular shape, and integral body has for example long limit (120～150mm) * minor face (80～100mm) * thickness (rectangular parallelepiped about 10～30mm).But for a person skilled in the art, this size and dimension obviously can change according to purposes and other requirements.Therefore, the surface configuration of the microplate 40 of the band filtrator of the present application for example, also can be circle or oval except oblong-shaped shown in Figure 7 and other rectangular shapes.But, in the following description, the microplate of above-mentioned rectangular shape is described.In this microplate 40, be the peristome 50 that disposes a plurality of (are 12 * 8 in the example at Fig. 7, amount to 96) on whole of upside in face side, nutrient solution (for example the blood of Cai Jiing etc. be examined material) supplied in the microplate 40 by these peristomes 50.
At length inquire into the structure of the microplate 40 of the present application below with reference to Fig. 8～Figure 12.Especially as shown in Figure 9, the microplate 40 of the present application has: the last cubitainer 41 that is formed with above-mentioned peristome 50; Periphery is chimeric and be fastened on the intermediate receptacle on the cubitainer 41 42 on this; Be configured in the lower reservoir 43 of the downside of this intermediate receptacle 42; Be clamped in last side seal 44 and the following side seal 45 gone up between cubitainer 41 and the intermediate receptacle 42; Be configured in the filtrator 46 of both allocations of the last side seal 44 that joins with last cubitainer 41; And be configured between above-mentioned intermediate receptacle 42 and the lower reservoir 43 auxiliary sealing member 47.
From Fig. 8 and Figure 10 as can be known, last cubitainer 41 and intermediate receptacle 42 have roughly the same sectional area, and lower reservoir 43 has the sectional area that is a bit larger tham these containers.In addition, as shown in Figure 9, last side seal 44 and following side seal 45 dispose in the mode that is enclosed in the cubitainer 41.And the filtrator 46 that is configured in the side seal 44 has and the roughly the same sectional area of above-mentioned peristome 50 sectional areas, in illustrated embodiment, has disposed 96 filtrators 46 altogether at the following quadrate part of peristome 50 correspondingly with peristome 50.And auxiliary sealing member 47 disposes in the mode that is enclosed in the intermediate receptacle 42.At this, last cubitainer 41, intermediate receptacle 42 and lower reservoir 43 form by having the stable chemical characteristic and the plastic material of elastically deformable (for example acrylic resin).
In addition, seal 44,45,47 is formed by the soft material with stable chemical characteristic (for example silicone) equally.And all these containers and seal are preferably formed by transparent material.Filtrator 46 for example can form by the etching silicon chip.In the following description, in order to understand structure easily, the part that seal 44,45,47 is divided into three splits is put down in writing, but, be not limited thereto, for example also can utilize unlike material to be shaped in advance or embedded shaping etc. is bonded on the top plate portion of lower reservoir 43 with auxiliary sealing member 47, improve assembling operation thus.In addition, keep form by changing filtrator, thus seal 44,45 is integrally formed and form monomer and realize the minimizing of number of spare parts and the facilitation of assembling operation.
To describe each inscape that forms the microplate 40 of being with filtrator in order below.
On whole of the last cubitainer 41 of Figure 13～shown in Figure 180, roughly dispose circular open portion 50 with required identical sectional area (Fig. 7～Fig. 9, Figure 12～Figure 14) equably.These peristomes 50 are as shown in Fig. 6 and Figure 12 are clear and definite, and both sinking with respect to vertical lower by the surface portion from last cubitainer 41 integratedly towards rear side, the cone shape wall 51 of sizing L1 (Fig. 9) forms.And, as Fig. 8～Figure 10, Figure 14, shown in Figure 16, whole outside on cubitainer 41 surfaces on this, both generally perpendicularly extending towards the below from this surface, the outer vertical wall 52 of sizing L2 (Fig. 9) (Fig. 9, Figure 18) forms.In addition, on the lateral surface of this outer vertical wall 52, be formed with outside projection 53 (Fig. 9, Figure 18) with circular section.This projection 53 is preferably formed on the lateral surface complete cycle of this outer vertical wall 52, still, also is not limited thereto, and also can be configured in off and on the complete cycle of this lateral surface.
In addition, as shown in Figure 9, the outside of the outer vertical wall 52 of cubitainer 41 is formed with flange 54 towards radial direction foreign side on this, and this flange 54 has the function of the end of protection intermediate receptacle 42.And, on the face that in the lower surface portion of above-mentioned each cone shape wall 51, joins, roughly forming a plurality of (being 2 in illustrated embodiment) downward contact ring 55 (Fig. 9 and Figure 15) on the concentric circles with last side seal 44.These ring 55 contacts go up side seal 44 upper surface, push side seal this on and play the function that prevents sealing part generation change in location.
As Figure 13, Figure 16 and shown in Figure 17, on the container 41, form a plurality of embedded holes 56 in the zone between peristome 50 up.In example shown in Figure 13, this embedded hole 56 from the right vertically on, the 2nd row and the 3rd row between, the 4th row and the 5th row between, the 6th row and the 7th row between, the 8th row and the 9th row between and the 10th row and the 11st row between, from above transversely, the 2nd row and the 3rd row between, the 4th row and the 5th row between, the 6th row and the 7th row between the zone in, be provided with 15 altogether, but be not limited thereto.Also can be provided with more or lessly.Especially as shown in figure 17, this embedded hole 56 is formed by the hole of the circular section that hangs down with certain diameter from the surface of last cubitainer 41.This hole is slightly smaller than the diameter of above-mentioned peristome 50 by diameter upper section 57, diameter than this upper section littler and diameter along with enlarge downwards and gradually below part 58 and the stage portion 59 of extending towards horizontal direction between part 57 and the below part 58 up constitute.Why diameter is in order easily stop pin 67 to be imported embedded hole 56 along with enlarging downwards and gradually to below part 58.As Fig. 9, Figure 13 and shown in Figure 180, on this surperficial outer peripheral edges of cubitainer 41 and peristome 50 around be formed for the small protrusion 77 strengthened respectively.
The intermediate receptacle 42 of Figure 19～shown in Figure 25 is provided with opposed to each other with the downside of last cubitainer 41, as Fig. 8～Figure 12 illustrates expressly, has and cooperates with last cubitainer 41 and the function of side seal 44 and following side seal 45 in the clamping.In addition, intermediate receptacle 42 also has the function that interconnects cubitainer 41 and lower reservoir 43.As Fig. 9, Figure 22 and Figure 25 illustrated expressly, intermediate receptacle 42 had from its neighboring towards last cubitainer 41 and erects wall 60 what the approximate vertical direction erected integratedly.As shown in Figure 9, this erects wall 60 and erects towards the position of the lateral surface that surrounds the above-mentioned outer vertical wall 52 that goes up cubitainer 41.Form the inside projection 61 that for example has the circular section at this medial surface that erects wall 60.The outside projection 53 of the outer vertical wall 52 of this projection 61 cubitainer 41 on being arranged at and should between the flange 54 of cubitainer 41, be formed on this on the position that the upper section of outer process 53 engages.
Thus, the outside projection 53 by making cubitainer 41 and the inside projection 61 of intermediate receptacle 42 are carried out chimeric, can realize that the tight inlay card of two containers closes.This projection 61 is preferably formed on this medial surface complete cycle that erects wall 60, but is not limited thereto.That is to say, also can only be arranged on as on the set position of the projection 53 of the last cubitainer 41 of chimeric object.Thus, go up the chimeric operation of cubitainer 41 and intermediate receptacle 42 easily, can realize the economization of materials used.This erects wall 60 and extends to than the position of this projection 61 more by the top and the degree of not joining with the flange 54 of last cubitainer 41.Thus, strengthen this and erect wall 60 and the stable chimerism of going up cubitainer 41 and intermediate receptacle 42.
And, as shown in Figure 9, near the outside of intermediate receptacle 42, be provided with integratedly towards the direction opposite and from this maintenance wall portion 62 that hangs down downwards with erecting wall 60.This maintenance wall portion 62 preferably hangs down in the mode of the outside integral body of surrounding this intermediate receptacle 42.This maintenance wall portion 62 of extending downwards as shown in Figure 9, preferably extends downwards from the inside part that erects wall 60 that extends upward.This is in order to satisfy the functional requirement of intermediate receptacle 42, not equal to be the requirement that is shaped in order to satisfy.In addition, on intermediate receptacle 42, when being assembled into this intermediate receptacle 42 on the cubitainer 41 be formed at this on the corresponding position of the peristome 50 of cubitainer 41, form the peristome 63 (Fig. 9, Figure 19 and Figure 23) of the circular section identical with the number (being 96 in illustrated embodiment) of this peristome 50.
As shown in Figure 9, the diameter of these peristomes 63 is slightly smaller than the diameter of the peristome 50 that is formed at cubitainer 41.This peristome 63 is made of thin petal-like guiding wall 64, and this guiding wall 64 hangs down towards the center position below of this peristome 63 integratedly from middle container 42.The below terminal part of these guiding walls 64 provides circular lower opening portion 65.The terminal part of this guiding wall 64 extends to the lower position that this retains the pars intermedia of portion 80 at least towards the direction of the portion that retains 80 of lower reservoir 43 described later, subject materials such as nutrient solution with the peristome 50 that will feed to cubitainer 41 are directed to the function of the portion of retaining 80 effectively, simultaneously, have and prevent that the subject material from dispersing with respect to this top wall that retains portion 80 and preventing to be housed in this and retain the function that the subject materials in the portion 80 flow backwards.And, in the upper surface of this intermediate receptacle 42, form on the peripheral part of each peristome 63, to be formed with the contact ring 66 (Figure 23) that a plurality of (being 2 in illustrated embodiment) make progress for concentrically ringed mode roughly with each peristome 63.These contact rings 66 contact, push sealing part 45 with the lower surface of following side seal 45 and play the function that prevents sealing part 45 generation change in location.
And, shown in Figure 11, Figure 22, Figure 24 A, Figure 24 B etc., on intermediate receptacle 42, with above-mentioned on the corresponding position of embedded hole 56 (Figure 13) of cubitainer 41, a plurality of (being 15 in illustrated embodiment) stop pin 67 erects and is formed on this position from the upper surface of this intermediate receptacle 42.These stop pins 67 preferably have the structure that is divided into a plurality of parts.That is, shown in Figure 24 A, stop pin 67 is made of a pair of sheet 68 that erects with symmetrical shape, and this is a pair of to erect the certain intervals 48 of sheet spaced-apart slots shape and erect.Form the hole enlargement part 69 with hole enlargement size on the top that respectively erects sheet 68, therefore, the top that erects sheet 68 has the roughly such shape of abacus pearl.Thus, producing on the top of this abacus pearl shape can be to the flexible elastic property of radial direction.
In the example of Figure 24 A and Figure 24 B, show this and erect the structure of sheet 68 for being divided into two, but be not limited thereto, for example also can be along the longitudinal have the slot part of set width dimensions, and the angle that slot part is set also can be every 60 or 120 degree or angle in addition every 90 degree settings.Mainly be to wish to come the feasible deflection that can not produce elastic property by slot part being set every equal angles.As shown in figure 17, the length of stop pin 67 for during these stop pins being assembled into the embedded hole 56 (Figure 11 and Figure 17) of cubitainer 41 time not from this on the surface of cubitainer 41 degree of giving prominence to.For stop pin 67 being easily mounted in the embedded hole 56 of cubitainer 41, as shown in figure 11, the below part 58 of embedded hole 56 forms from descending up the shape of undergauge gradually, thus, if stop pin 67 is pressed into embedded hole 56, thus then the hole enlargement part 69 of stop pin 67 flexibly gradually undergauge can easily be pressed in the embedded hole 56.
In addition, if the hole enlargement part of this stop pin 67 69 arrives the stage portion 59 of embedded hole 56, then hole enlargement part 69 is utilized elastic force and hole enlargement automatically, thereby is engaged to easily on this stage portion 59.In Figure 11 and embodiment shown in Figure 17, thereby stop pin 67 carries out chimeric can't the separation with respect to embedded hole 56 with so-called fixed installation state, but, find that in for example assembling back filtrator goes wrong when waiting, needing should bad filtrator taking-up and change.Therefore, also can suitably regulate the width dimensions of the vertical slot part that is formed at above-mentioned hole enlargement part 69, so that can as required stop pin 67 be separated from embedded hole 56.
Around each guiding wall 64 that hangs down from middle container 42, with the contact ring 70 of downward a plurality of (being 4 layers in the example of Fig. 9) substantially elliptical that is shaped of the mode of surrounding these guiding walls 64.The configuration state of contact ring 70 is illustrated in Fig. 9, Figure 23 and shows among the Figure 20 at the back side of intermediate receptacle 42.Thereby these contact rings 70 contact, push from the top this auxiliary sealing member 47 and play auxiliary sealing member 47 is correctly navigated to set locational effect with the upper surface of auxiliary sealing member 47 described later.At this, why the configuration with this contact ring 70 is made as substantially elliptical, is because the peristome 94 of this auxiliary sealing member 47 has substantially elliptical equally.A plurality of contact rings 70 are not limited to 4 layers, and this situation with above-mentioned contact ring 55,66 is identical.
Two seal members of clamping between cubitainer 41 and the intermediate receptacle 42 on above-mentioned, as shown in Figure 9, the last side seal 44 that to be upper surface kept by last cubitainer 41 and the back side are by the following side seal 45 of intermediate receptacle 42 maintenances.These seals by roughly the same surface area, thickness and roughly the same material for example soft material such as silicone form, thus, container 41 and intermediate receptacle 42 are pushed respectively and are kept when the seal 44,45 up, be formed at each contact ring 55,66 engaging-in sealing part that constitute by soft material of cubitainer 41 and intermediate receptacle 42 respectively, stop the shift in position and the seal shift in position each other of seal 44,45 thus.The neighboring of these seals 44,45 contacts on the medial surface of the outer vertical wall 52 of container 41 up and keeps and be positioned at this place as previously mentioned.
Below these seals 44,45 are described.
On the last side seal 44 as Figure 33～shown in Figure 37, with above-mentioned on form a plurality of (being 96 in illustrated embodiment) circular peristome 71 (Figure 33) respectively on peristome 50 corresponding each position of cubitainer 41.These peristomes 71 connect seal 44 self ground and form.And, be provided with the stage portion 72 (Figure 34 and Figure 36) of rectangular shape in the rear side of the sealing part 44 of the periphery of these peristomes 71.The shape of this stage portion 72 is roughly consistent with the shape and the gauge of filtrator 46 described later with the degree of depth.In illustrated embodiment, because hypothesis is the filtrator of rectangular shape, so stage portion 72 is rectangular shapes, still, under the situation of using round-shaped filtrator, what stage portion 72 was a diameter greater than peristome 71 is round-shaped.The diameter of preferred peristome 71 is roughly the same with the diameter that the bottom limited that is formed at the above-mentioned upward cone shape wall 51 of cubitainer 41.This is for the leakage that prevents to be examined material etc., can carries out the inspection of not having waste.
Between the peristome 50 of sealing part 44, form a plurality of (being 15 in illustrated embodiment) and connect sealing sized opening 73 part, that size is a bit larger tham this peristome 50.This opening 73 is used to accept the below part 58 (Figure 17) of the embedded hole 56 that is formed on the cubitainer 41.And the bight of side seal 44 is provided with the recess 74 of location usefulness on this.This recess both is formed on as shallow recess on the allocation as shown in figure 37.In illustrated embodiment, at the upper surface (Figure 33) of seal 44 and the different bights of the back side (Figure 34) recess is set, but is not limited thereto.Also can respectively be provided with one in four bights of upper surface and lower surface, be provided with 8 altogether, perhaps also can only be provided with one at single face.Its reason is that these recesses 74 have the function of special datum point when filtrator 46 being equipped on the peristome 71 of sealing part 44, and the difference of carrying mechanism because of filtrator requires various reference points.
On following side seal 45 shown in Figure 38, with last side seal 44 similarly, with above-mentioned on also form a plurality of (being 96 in illustrated embodiment) circular peristome 75 respectively on peristome 50 corresponding each position of cubitainer 41.The diameter of preferred these peristomes 75 is also roughly the same with the diameter that the bottom limited that is formed at the above-mentioned upward cone shape wall 51 of cubitainer 41.Between the peristome 75 of this time side seal 45, a plurality of (being 15 in illustrated embodiment) opening 76 that size is a bit larger tham the size of this peristome 75 is set with also connecting the sealing part.This opening 76 is used for accepting the below part 58 (Figure 17) of the embedded hole 56 that is formed on cubitainer 41.
Though following side seal 45 has the size and dimension roughly the same with last side seal 44, but stage portion such on the back side of side seal 44 72 is not set, peristome 75 and opening 76 are the states that penetrate into the back side from upper surface, therefore, the upper surface of following side seal 45 and the back side have roughly the same shape.In addition, this time side seal 45 has the thickness roughly the same with last side seal 44.In illustrated embodiment, consider the operation when assembling is used to keep the stage portion 72 of filtrator 46 and stage portion 72 is arranged on side seal 44, still, also this stage portion 72 can be arranged on down on the side seal 45 and at this place and accommodate filtrator 46.And, also the stage portion with only about half of gauge of filtrator 46 peripheral parts can be arranged on respectively on side seal and the following side seal, both be fixed on by two seal clamping filtrators 46 on the allocation.
Below with reference to Figure 26～Figure 32 lower reservoir 43 is described.On lower reservoir 43, with above-mentioned on be formed with on the peristome 50 corresponding positions of cubitainer 41 a plurality of (being 96 in illustrated embodiment) from the surface of this lower reservoir 43 portion that retains 80 (with reference to Fig. 9, Figure 12, Figure 29) that hangs down of one generally perpendicularly.These retain portion 80 and have following function, promptly after utilizing 46 pairs of filtrators to supply to being examined material and filtering of peristome 50 of cubitainer 41, only receive and accommodate the inspection sample after this filtration.Therefore, this retains portion 80 size for the volume of the inspection sample that can guarantee to accommodate necessary amount.And, retain the periphery flank 81 that portion 80 extends downwards than this in that the peripheral part of this lower reservoir 43 is integrally formed.This periphery flank 81 by the surface from lower reservoir 43 extend to first of foreign side below hang down portion 82, further extend downwards via the stage portion of approximate horizontal expansion from this first bottom of hanging down portion 82 second hang down portion 83 and the 3rd portion 84 that hangs down of further extending via the stage portion of approximate horizontal expansion from this second bottom of hanging down portion 83 constitutes downwards.
These periphery flanks 81 have stacked can be when promptly piling up the microplate 40 of a plurality of the present application with the stacked function of stable status.That is to say, as shown in figure 27, the 3rd the hang down horizontal inside dimensions L3 of portion 84 and lateral dimension L5 and the longitudinal size L5 that longitudinal medial size L4 is substantially equal to last cubitainer 41 shown in Figure 13 respectively, and, stable stacked in order to guarantee, as shown in figure 27, hang down the 3rd and form a plurality of stacked flanks 85 (being 10 in illustrated embodiment) on the interior sidewall surface of portion 84.Thereby engaging to keep with the neighboring of last cubitainer 41, these stacked flanks 85 make stacked stable status when stacked.In addition, first the hang down physical dimension of portion 82 is slightly littler than the inside dimensions of the maintenance wall portion 62 of intermediate receptacle 42, as shown in Figure 9, provides both can closely chimeric relation basically.
As previously mentioned, on lower reservoir 43, form a plurality of portions 80 that retain.From above observe these and retain under the situation of portion 80, as shown in figure 26, a pair of ventilating part 90 forms on this retains the diametric(al) of portion 80 opposed to each other.As shown in figure 30, these ventilating parts 90 are formed on the neck hole enlargement inclined-plane 92 that the upper surface 91 of container 43 extends downwards of the portion of retaining 80, and extend on the direction with respect to these upper surface 91 approximate vertical.As a result, the top open end of ventilating part 90 is communicated with hole enlargement inclined-plane 92 and opens to this place.Therefore, can make the inside of the portion that retains 80 shown in curve 93, become decompression state via ventilating part 90 by giving negative pressure from the top of the portion of retaining 80 to the outside of the portion of retaining 80.Figure 31 represents hole enlargement inclined-plane 92 and the upper end ventilating part 90 to this place's opening.In addition, Figure 32 represents the outside opened state of ventilating part 90 to the portion of retaining 80.Figure 27 represents from the figure of beneath lower reservoir 43.
At this, retaining portion 80, to illustrate than the portion that retains 80 shown in Figure 26 be greatly because Figure 27 represents the outer diameter part of the portion of retaining 80 and Figure 26 represents the inside diameter of the portion of retaining 80.In Figure 27, represent from beneath hole enlargement inclined-plane 92 and state when being formed on this ventilating part 90 to the outstanding part of radial direction foreign side opposed to each other from the outer diameter part of the portion of retaining 80 along diametric(al).In Figure 30 and Figure 31, the shape that retains portion 80 is more or less different.That is to say that in Figure 30, the portion integral body of retaining forms narrowly, and in Figure 31, the portion that retains integral body than Figure 24 forms widely.This be because, as its profile direction shown in Figure 26, Figure 30 represents along the section of the direction observation that connects a pair of ventilating part, be will the portion of retaining in order to form the hole enlargement inclined-plane to press to the state representation of this bevel direction, and Figure 31 represents not have the influence on the hole enlargement inclined-plane that forms the portion that retains along the section of observing with the direction of the direction quadrature that is connected a pair of ventilating part.The situation that this pass ties up to Figure 12 and Fig. 9 also is same.
Between intermediate receptacle 42 and lower reservoir 43, carrying auxiliary sealing member shown in Figure 39 47.Auxiliary sealing member 47 is by forming with the roughly the same material of last side seal 44 and following side seal 45, and thickness and area are also roughly the same with these seals, and, also identical at the number of the peristome 94 of this formation.Wherein, peristome 94 is shaped as the identical substantially elliptical of configuration shape with the contact ring 70 that is arranged on intermediate receptacle 42 back sides.But size will be slightly smaller than contact ring 70.This is because as can be seen from Fig. 9, this peristome 94 than the position of contact ring 70 more in the inner part.In addition, why this peristome 94 being formed substantially elliptical, is to be formed on the portion that retains 80 ventilating part 90 on every side that is arranged on the intermediate receptacle 42 in order not stop up.Peristome 94 penetrates into ground, the back side from upper surface and forms, so the upper surface of auxiliary sealing member 47 and the back side have roughly the same shape.
Filtrator 46 (Fig. 9) has the effect that material only screens necessary key element and sends into the portion that retains 80 of lower reservoir 43 that is examined from the peristome 50 that supplies to cubitainer 41, usually, in order to gather accurately but be provided with the filtering material of uniform through hole as the predetermined substance of target.In general, form shape by the etching silicon chip.The diameter in the hole of filtrator is corresponding to the size of required predetermined substance and determine.In addition, in order to shorten filtration time, filtrator 46 is extremely thin filminess.For this reason, be easy to generate damage, so when handling, want extreme care careful.But, be fixed in the part of microplate, promptly in illustrated embodiment, be housed in be formed at Figure 36 on the part of stage portion 72 on the side seal 44, as Fig. 9 with Figure 12 shows that thick film.Filtrator 46 forms the rake 49 that tilts gradually to the film portion of central authorities from the peripheral part of the wall thickness of accommodating stage portion 72.In illustrated embodiment, this filtrator 46 has rectangular shape, and the stage portion 72 that is used to accept the last side seal 44 of this filtrator equally also has rectangular shape, but as previously mentioned, is not limited thereto.
When the microplate of band filtrator of assembling the present application, at first, the inversion state that last cubitainer 41 is configured to overturn.Then, last side seal 44 is arranged on the last cubitainer 41 of this inversion state.At this moment, the stage portion 72 that should be noted in the discussion above that side seal 44 is provided with in the mode towards the top.Then, filtrator 46 is arranged on the stage portion 72 of side seal 44 on this.At this moment, filtrator 46 is with state (inversion state) configuration opposite with when work.Thin in the extreme owing to filtrator 46 integral body, especially fragile in the extreme at the circular middle body at peristome 71 places that are disposed at Figure 34, therefore, when handling, require to cause attention to greatest extent.In filtrator 46, never utilize rake 49 to be connected to the part between the thick slightly peripheral part that is housed in the stage portion 72 with the thin part of the central authorities of this peristome 71.After this, following side seal 45 is configured in the upper surface of side seal 44 and filtrator 46 on this.Be formed in the stage portion that is being used to keep filtrator 46 under the situation of side seal down, on being provided with after the side seal, be positioned on this on side seal in advance filtrator 46 being arranged on following side seal on this stage portion.At this, last side seal 44 and following side seal 45 all suitably are configured in the inboard of the outer vertical wall 52 of cubitainer 41.
As other order, also can be after carrying filtrator 46 on the side seal 44 in advance, the last side seal 44 of this band filtrator is set on the last cubitainer 41 that is inversion state.Therefore, recess 74 is arranged on the different bights of the upper surface and the lower surface of side seal 44 on this, can suitably control the position of side seal 44.That is to say,, as required, require the reference position of sealing part 44 is finely tuned for never illustrated filtrator is carried the appropriate location that the continuous filtrator of supplying with 46 of mechanism is configured to side seal 44.In this case, act on this recess 74, filtrator 46 always can be configured in the appropriate location of side seal 44 by making suitable position control mechanisms such as indicator.After side seal is configured on the cubitainer 41 on will be with filtrator, carry side seal 45 down, the moving of restriction filtrator.
Then, intermediate receptacle 42 is arranged on down the upper surface of side seal 45.At this moment, this intermediate receptacle 42 is located in the mode that erects side seal 45 under the wall 60 downward also coverings with projection 61, to push intermediate receptacle 42 chimeric fully with the projection 53 on the outer vertical wall 52 that is arranged on cubitainer 41 until the projection 61 of this intermediate receptacle 42 for container 41 sides upward, confirms that then intermediate receptacle 42 and last cubitainer 41 are chimeric effectively at periphery area.Then, 15 stop pins 67 that are arranged at intermediate receptacle 42 are inserted the embedded hole 56 of going up cubitainer 41.The diameter dimension of stop pin 67 is a bit larger tham the diameter dimension of the below part 58 of embedded hole 56.But, by stop pin 67 forcibly being pressed into this below part 58 of undergauge gradually, rely on center position thereby the top with abacus pearl shape that makes this stop pin 67 utilizes the space with interval 48 undergauge takes place, therefore, stop pin 67 is embedded in this below part 58 easily.And if stop pin 67 is pressed in the embedded hole 56, then stop pin 67 arrives the upper section 57 with hole enlargement size.Therefore, return to common diameter state for the top of the stop pin 67 of undergauge state before this.Therefore, this stop pin 67 is supported by the stage portion 59 of embedded hole 56 and stops stop pin to be deviate from from this.Thus, need to confirm that stop pin 67 is fitted in the stage portion 59 effectively.This can easily determine along axis direction moves up and down, stop pin sends when being embedded on the stage portion sound by making stop pin.
In the moment after projection 61 on the wall 60 and stop pin 67 are assembled into the appropriate location of erecting that will be set in place, finish cubitainer 41, intermediate receptacle 42, go up side seal 44, the assembling of side seal 45 and filtrator 46 down in the neighboring of intermediate receptacle 42.Under this state, the contact ring 55 of lower surface that is arranged on the cone shape wall 51 of cubitainer 41 is pressed on the side seal 44, and the contact ring 66 that is arranged on the peristome periphery of intermediate receptacle 42 is pressed into down on the side seal 45.And these seals 44,45 constitute by soft materials such as silicone and are the states of mutual crimping.Therefore, can stop the offset of filtrator 46 fully.In addition, last cubitainer 41 and intermediate receptacle 42 utilize the undercutting pawl chimeric and embedded hole 56 and stop pin 67 of projection 53,61 chimeric and realize practical closely chimeric respectively.
Then, auxiliary sealing member 47 suitably is installed on the zone that the maintenance wall portion 62 of extending towards the top by the intermediate receptacle 42 that is in inversion state limited.At this moment, auxiliary sealing member 47 utilizes the contact ring 70 of peristome 63 peripheries (being 4 layers in illustrated embodiment) that are arranged on intermediate receptacle 42 to remain on the desirable position.Lower reservoir 43 is installed at last.With respect under inversion state, keeping wall portion 62 intermediate receptacle 42 up, lower reservoir 43 is similarly carried with the inversion state of upset.At this moment, assemble with the mode that the inside surface of maintenance wall portion 62 on being arranged on intermediate receptacle 42 closely contacts to be arranged at first of lower reservoir 43 outside surface of portion 82 that hangs down, thereby finish assembling operation.At last, the microplate 40 of finishing the band filtrator of assembling operation is turned back to use location shown in Figure 8.
At this moment, as shown in Figure 9, be formed on funnelform guiding wall 64 loosening being configured in the portion that retains 80 of lower reservoir 43 on the intermediate receptacle 42 chimericly.In the moment of having finished assembling, can stackedly in order take care of microplate 40, in addition, at this moment, under stacked situation, be arranged on flange 54 on the last cubitainer 41 of below microplate the 3rd the hanging down on the inboard stacked flank 85 of portion 84 of nearest microplate above being arranged on, thereby can keep microplate effectively.Therefore, can be in order with stable posture with stacked each microplate of multilayered state.Microplate for can in a large number production the present application can apply telltale mark on each part, so that assembly mechanical can be with reference to the position of each part and the reference after carrying out the correct location of each part.Thus, can realize the robotization of assembling operation, can produce in a large number.Especially, in the application's microplate, because flimsy filtrator 46 is important factors in assembling operation with respect to the positioning operation of last cubitainer 41, so recess 74 that applies position adjustment usefulness at the upper surface and/or the lower surface of last side seal 44, so that can suitably locate the last side seal 44 with stage portion 72 that the filtrator of accepting this filtrator 46 accepts usefulness, this is very important.
Next, the using method to the microplate 40 of the band filtrator of the embodiment 2 of the present application describes.As shown in Figure 8, the microplate 40 with a band filtrator is configured on both allocations of level.Then, utilize suitable utensils such as suction pipe will be examined the peristome 50 that material supplies to cubitainer 41.In the microplate 40 herein, can be that 96 corpse or other object for laboratory examination and chemical testing are gathered as the inspection sample simultaneously with the number of peristome 50.Fig. 9 below with reference to the section that amplifies expression peristome 50 describes, supply in the peristome 50 that the cone shape wall 51 by last cubitainer 41 limits be examined that material drips to that stage portion 72 by last side seal 44 is kept and the thin filtering surface by the central authorities of the filtrator 46 that descends side seal 45 clampings on.At this, can only make inspection sample by this filtrator 46 with set characteristic.Garbled like this inspection sample is guided by the funnelform guiding wall 64 of intermediate receptacle 42 and accommodates in the portion that retains 80 of lower reservoir 43.Should be noted in the discussion above that the amount of accommodating the inspection sample in the portion of retaining 80, is the amount that is housed in the space that the usefulness size L7 between the lower opening portion 65 of bottom to the lower end of guiding wall 64 of the portion of retaining 80 represents.Concrete sample size maximum is about about 30 cubic millimeters.
Accommodated a certain amount of inspection sample in the portion of retaining 80 after, only the lower reservoir 43 with microplate 40 separates from middle container 42.This detached job can easily carry out to being pulled away from towards upper and lower by catching the hang down wall 60 of erecting of portion 83 and intermediate receptacle 42 of second of lower reservoir 43.Noting not making the portion that the retains 80 interior inspection samples of the lower reservoir that is housed in such separation to overflow on one side, should check that sample be transported to set inspecting stand on one side, in the detailed inspection of this place's beginning sample.
Because the relation of the mesh size of the filtrator 46 that uses in the present application, can predict that sometimes checked property blames in by filtrator 46, be examined material owing to for example with contacting of air the danger that rerum natura changes takes place during this time.For this reason, in the microplate 40 of the present application, in order promptly to finish filtration operation enough as far as possible with respect to checked property mass-energy, be provided with and provide negative pressure to the portion that retains 80 of lower reservoir 43, make and be examined the mechanism that material forcibly moves in the portion of retaining 80 by filtrator, so that the checked property mass-energy that supplies to peristome 50 is enough promptly by filtrator 46.That is to say, reduce pressure by known mechanisms of decompressor such as pumps around the portion that retains 80 of the microplate 40 of the band filtrator of the present application.As a result, shown in the Reference numeral among Figure 30 93, thereby the air that retains portion 80 inside reduces the pressure that retains portion 80 by the outside that a pair of ventilating part 90 is discharged to the portion of retaining 80.Like this, become negative pressure owing to retain the inside of portion 80, so shown in the Reference numeral among Fig. 9 95, the air in the peristome 63 of the intermediate receptacle 42 that the downside of filtrator 46 is nearest attracted to outside the portion of retaining 80, thereby this peristome 63 becomes negative pressure.Like this, supply to cubitainer 41 peristome 50 be examined material promptly by filtrator 46 attracted to the below.As a result, be examined material by filtrator 46 being forced to property move in the portion of retaining 80.At this moment, should be noted in the discussion above that as mentioned above, make the liquid level of accommodating the material in the portion of retaining 80 not rise to more than the size L7.In other words, must between the lower opening portion 65 of the liquid level of sample and guiding wall 64, the space be set.Under the situation that does not have this space, this liquid directly is attracted in the reliever, thereby can't realize initial purpose.
Figure 40 A and Figure 40 B represent to be used for air with the portion of retaining 80 inside be discharged to the portion of retaining 80 the outside ventilating part 90 number, this air hole 90 the installation site and retain relation between the position of the guiding wall 64 in the portion 80.In Figure 40 A and Figure 40 B, the number of A (a), B (b), C (c) expression ventilating part 90 is one embodiment, and D (d), E (e) expression is provided with the embodiment of two ventilating parts 90 opposed to each other along diametric(al).And a, b, d represent that this ventilating part 90 is arranged on the embodiment on the circumscribed circle 96 that each angle with quadrilateral filtrator 46 joins, and c, e represent that ventilating part 90 is arranged on the embodiment of the inboard of this circumscribed circle 96.
In more detail, in the embodiment of A (a), retain the length-wise axis 98 of portion 80 and the length-wise axis 99 of guiding wall 64 and be on the same axis, when decompression, in the portion of retaining 80, the space that air moves is roughly the same around guiding wall 64.In the embodiment of B (b), guiding wall 64 is near a side opposite with the side that ventilating part 90 is set.In the embodiment of C (c), ventilating part 90 near retain that portion 80 is provided with and with the embodiment of B (b) similarly guiding wall 64 near sides opposite with the side that ventilating part 90 is set.In the embodiment of D (d), though identical with the embodiment of A (a), be provided with along the opposed a pair of ventilating part 90 of diametric(al).At last, in the embodiment of E (e), with the embodiment of A (a) and D (d) similarly, the length-wise axis 99 that retains the length-wise axis 98 of portion 80 and guiding wall 64 is consistent, but will be along the opposed a pair of ventilating part 90 of diametric(al) near retaining portion's 80 ground settings.
According to the present application people's experiment as can be known, the position of ventilating part 90 is more little the closer to 80 decompression losses of the portion of retaining.But, also find owing to the intensity that reduces pressure, retain the reasons such as amount of liquid in the portion 80, there is sizable deviation.In a～e of Figure 40 A and Figure 40 B, be positioned at more than the circumscribed circle of rake 49 of circle 97 expression filtrators 46 of circumscribed circle 96 inboards.In addition, in A～E of Figure 40 A and Figure 40 B, the profile line that retains in the portion 80 is represented sample, and its amount is for about about 30 cubic millimeters.In illustrated embodiment, represented that ventilating part 90 is one and two s' a situation, but also more ventilating part can be set, also can give play to same good effect being provided with under 4,6 the situation.
[embodiment 3]
With reference to Figure 41 the embodiment 3 of the present application is described below.Embodiment that Figure 41 represents 3 is with described embodiment 2 is similar before, so following only to stressing with described embodiment is different before part.In Figure 41, filling A behind the Reference numeral of before embodiment represents with similar key element of embodiment before or part.In addition, from embodiment shown in Figure 41 as seen, each key element that constitutes Figure 41 has and accompanying drawing before, the different slightly shape of key element especially shown in Figure 9, but, the basic structure of each key element is identical with embodiment before basically, but be the variation that those skilled in the art can be readily appreciated that, so omit detailed icon to each inscape of embodiment 3.
In the microplate 40A of band filtrator shown in Figure 41, flange rising portions 86 from the flange 54A that constitutes the periphery of going up cubitainer 41A, preferably with this on cubitainer 41A erect to vertical direction integratedly.Similarly, opening rising portions 87 from the periphery of each peristome 50A, preferably with this on cubitainer 41A erect towards the top integratedly.At this, the height of the height of preferred flange rising portions 86 and opening rising portions 87 is substantially the same or flange rising portions 86 is higher.This be for, when when carry waiting, a plurality of microplate 40A being piled up each other, a plurality of microplate 40A can not piled up not produce bias and not produce the steady state (SS) of rocking mutually.
By the integral body that flange rising portions 86 and opening rising portions 87 are set have increased microplate 40A height is set, the height dimension L1 of peristome shown in Figure 3 50 is increased in fact, the result handles this microplate 40A easily.In addition, the feasible operation to this peristome 50A supply sample of the area of the 50A of enlarged openings portion becomes easy.In the embodiment shown in fig. 9, the size L2 of outer vertical wall 52 is a bit larger tham the size L1 of cone shape wall 51, the result utilizes both difference in size, and outer vertical wall 52 is in the lower position of cone shape wall 51 can be enclosed in seal 44,45 zone that this vertical wall 52 limited; And in embodiment shown in Figure 41, but be that the inboard rising portions 88 that utilizes intermediate receptacle 42A to be provided comes sealing part 44A, 45A are surrounded.Bottom at cone shape wall 51A is arranged on the contact ring of representing with Reference numeral 55 among Fig. 9 equally.
The downside of intermediate receptacle 42A and last cubitainer 41A is provided with opposed to each other, and cooperate with last cubitainer 41A and the function of side seal 44A and following side seal 45A in the clamping is provided, this intermediate receptacle 42A has the function that interconnects cubitainer 41A and lower reservoir 43A.As shown in figure 41, intermediate receptacle 42A has from its neighboring towards what last cubitainer 41A erected on the approximate vertical direction integratedly and erects wall 60A.This erects wall 60A and erects towards surrounding the above-mentioned upward position of the lateral surface of the outer vertical wall 52A of cubitainer 41A.And intermediate receptacle 42A preferably has integratedly the inboard that erects to the position that the medial surface with the outer vertical wall 52A of last cubitainer 41A joins at complete cycle and erects wall 88.Thus, the outer vertical wall 52A of last cubitainer 41A closely is entrenched in the slot part that is defined between the internal face that erects wall 60A and the inboard outside wall surface that erects wall 88.Like this, chimeric stronger between last cubitainer 41A and the intermediate receptacle 42A.It is lower than the height that erects wall 60A that this inboard erects wall 88, and the outside upper section that erects wall 88 in this inboard has carried out the angle slightly to be cut, thereby carries out last cubitainer 41A is embedded the operation of this slot part easily.The inboard erects wall 88 and also has the function that the outer peripheral edges of side seal 44A and following side seal 45A are gone up in the coupling maintenance.Therefore, this inboard erects wall 88 and has the height that its medial surface height is enough to keep these seals 44A, 45A.
In the embodiment shown in fig. 9, the outer peripheral edges of these seals 44,45 are kept by the medial surface of the outer vertical wall 52 of last cubitainer 41, and in embodiment shown in Figure 41 3, it but is the medial surface maintenance that erects wall 88 by the inboard of intermediate receptacle 42A, in this, two embodiment are different.As a result, when side seal is with following side seal in assembling, in the embodiment of Fig. 9, seal 44,45 is assembled on the last cubitainer 41 of inversion state, then intermediate receptacle 42 is assembled on the cubitainer 41; And in the embodiment 3 of Figure 41, after being assembled into seal 44A, 45A on the intermediate receptacle 42A, just this intermediate receptacle 42A is assembled on the last cubitainer 41A that is inversion state.And than the seal 44,45 that uses in embodiment 2, the thickness of seal 44A, the 45A that uses in embodiment 3 is little, and little amount is the thickness that the inboard erects wall 88.
Near the outside of intermediate receptacle 42A, be provided with and erect wall 60A integratedly in the opposite direction and from this maintenance wall 62A of portion that hangs down downwards.This maintenance wall 62A of portion has chimeric intermediate receptacle 42A and lower reservoir 43A and keeps the function of auxiliary sealing member 47A.In addition, intermediate receptacle 42A with the corresponding position of peristome 50A of last cubitainer 41A on have the guiding wall 64A identical integratedly with the quantity of this peristome 50A.Guiding wall 64A has the function that is used for the sample that supplies to peristome 50A and filtered by filtrator 46A subsequently is directed to the 80A of the portion that retains of lower reservoir 43A.In addition, between maintenance wall 62A of portion and guiding wall 64A, be formed for auxiliary sealing member 47A is remained on both contact ring 70A of allocation.This structure is identical with embodiment 2 shown in Figure 9.In embodiment shown in Figure 41 2, intermediate receptacle 42A at contact ring 70A and keep also having integratedly between the wall 62A of portion towards below outstanding protuberance 89.
Except said structure, embodiment 3 shown in Figure 41 is substantially the same with the embodiment 2 described structures shown in Fig. 7～Figure 40 B, and using method is also identical with embodiment 2 described methods.
In addition, in the present application, owing to have a plurality of peristomes, so for each position of specific each peristome, the on-site mark of expression is set in the position of the peristome of adjacent upper portions container and/or bottom container.Specifically, as shown in Figure 1, vertical mark A of vessel surface, B, C etc. and at horizontal mark 1,2,3 etc. up, thereby each position that can specific each peristome.In addition, in lower reservoir, as shown in figure 42, for from above correctly identification A, B, C and 1,2,3 etc. under the vessel surface side situation of observing, for example process can face literal in the rear side of lower reservoir.
And, rocking in order to prevent from intermediate receptacle 42 and lower reservoir 43 are set, and when when carrying etc., piling up a plurality of microplates mutually, can pile up these microplates not produce mutual bias and not produce the steady state (SS) of rocking, as shown in figure 43, point-like is provided with a plurality of lower reservoir flanks 30 on the side that joins with intermediate receptacle 42 of lower reservoir 43.The lower reservoir flank 30 that is provided with for example is provided with 3 places on the length direction of the microplate of being with filtrator, 2 places are set on short side direction.
Similarly, rocking in order to prevent from lower reservoir 43 and auxiliary sealing member 47 are set, as shown in figure 43, preferably the end face complete cycle of the part of joining in the periphery with auxiliary sealing member 47 of lower reservoir 43 is provided with lower reservoir guide portion 35.
Utilizability on the industry
The filter ground that the microplate with filter of the present application can not damage crisp fritter safely Set filter, thereby the low price of microplate self only will have been accommodated inspection afterwards easily in filtration The pallet of looking into sample separates simply and sample is offered next worker who carries out quality analysis etc. Order. And in the present application, the degree of depth that retains section is dark, and, sample is supplied to This length that retains the guiding wall in the section is long, thereby even has applied big bearing when checking attraction Press, can prevent that also sample from mixing mutually with adjacent sample, and can when checking attraction, prevent Sample produces and flows backwards, and, can also prevent that this sample from attracteding to outside the section of retaining.
Therefore, the requirement of effectively answer up filtration, thus can realize examining rapidly Look into attraction, thus, can be to carry out the analysis of a large amount of samples than the in the past shorter time, in addition, Organize inspection and other fields to use safely in cell tissue cultivation or live body cultivation. And And, owing to can use than the in the past filtration of bigger negative pressure, so provide a kind of can Tackle effectively to a large amount of samples carry out requirement that rapid and correct sample analyzes, in industry On very useful invention.
Claims (26)
1. the microplate with filtrator (110) comprising:
Last cubitainer (111) has and is used to inject the peristome (120) that is examined material;
Last side seal (113) and following side seal (114) are keeping filtrator (115);
Link parts (112), be and the above-mentioned binding parts (112) of going up cubitainer (111) tabling, have the peristome (140) that the inspection sample that passed through filtrator (115) is passed through, linking parts (112) and should go up that cramping keeps upward side seal (113) and following side seal (114) between the cubitainer (111);
Lower reservoir (116) is to have the lower reservoir (116) of accommodating the portion that retains (160) that checks sample, and this lower reservoir (116) is to be maintained with respect to linking parts (112) dismounting mode freely.
2. the microplate of band filtrator as claimed in claim 1 is characterized in that, last cubitainer (111) has the outer vertical wall (124) of extending to vertical lower at outer peripheral portion, and this outer vertical wall (124) has the outstanding projection (125) towards foreign side;
Link parts (112) and have to what vertical direction was extended at outer peripheral portion and erect wall (135), this erects wall (135) and has the outstanding projection (136) in side inwardly;
These projections (125,136) engaging, thus realize going up cubitainer (111) and link the chimeric of parts (112).
3. the microplate of band filtrator as claimed in claim 1 or 2, it is characterized in that, last cubitainer (111) has the embedded hole (128) that is made of upper section (129) and below part (130), upper section (129) has the hole enlargement hole, below part (130) has the undergauge hole, forms stage portion (131) between part (129) and the below part (130) up;
Link the stop pin (143) that parts (112) have the hollow shape of extending towards the top, this stop pin (143) has hole enlargement part (144) on top, and this hole enlargement part (144) has a plurality of slot parts that extend along axis direction;
Be pressed into this stop pin (143) by below part (130) to embedded hole (128) from last cubitainer (111), the hole enlargement part (144) of this stop pin (143) moves and undergauge to center position, by further being pressed into, stop pin (143) arrives upper section (129), this hole enlargement part (144) is engaged on the stage portion (131) of cubitainer (111), thus, cubitainer (111) and link the chimeric of parts (112) in the realization.
4. as the microplate of each described band filtrator in the claim 1 to 3, it is characterized in that, link parts (112) and have maintenance wall portion (137) and the register pin (145) that extends towards the below, this maintenance wall portion (137) is made of outside surface (138) and inclined inner surface (139) and is configured in outer peripheral portion to surround the mode that links parts (112), register pin (145) has cone shape, and, be provided with a plurality of register pins (145) at the inside part that links parts (112);
Lower reservoir (116) has periphery flank (161) that is made of rake (162) and step-like (163) and the register pin carrier (164) that is cone shape at outer peripheral portion;
By making lower reservoir (116) join with airtight conditions and binding parts (112) with respect to inclined inner surface (139) crimping rake (162), it is loosening chimeric that register pin (145) and register pin carrier (164) separate set compartment of terrain.
5. as the microplate of each described band filtrator in the claim 1 to 4, it is characterized in that, filtrator (115) is made by the etching and processing silicon chip, is made of the central part of the through hole with a plurality of size homogeneous and the peripheral part of this central part of encirclement, and peripheral part forms thicklyer than central part.
6. as the microplate of each described band filtrator in the claim 1 to 5, it is characterized in that, in the rake (162) of inclined inner surface (139) that links parts (112) and lower reservoir (116) one on be embedded with O shape and encircle etc. and can carry out airtight parts, thus, both are chimeric airtightly.
7. the microplate of band filtrator as claimed in claim 4, it is characterized in that, linking parts (112) and lower reservoir (116) locates chimeric airtightly at separately inclined inner surface (139) and rake (162), in other parts then is loosening chimeric, by the mechanism of decompressor being connected to a register pin carrier (164), can make the below of filtrator become negative pressure, thus, can shorten filter time.
8. as the microplate of each described band filtrator in the claim 1 to 7, it is characterized in that last side seal (113) and following side seal (114) are the structure of one.
9. as the microplate of each described band filtrator in the claim 1 to 8, it is characterized in that, on the parts of filtrator (115) or this filtrator (115) institute butt, be applied with the mark that is used for the particular group holding position.
10. the microplate with filtrator (40,40A) comprising:
Last cubitainer (41,41A) possesses and is used to inject the peristome that is examined material (50,50A);
Last side seal (44,44A) and following side seal (45,45A) are keeping filtrator (46,46A);
Intermediate receptacle (42,42A), be and the above-mentioned intermediate receptacle of going up cubitainer (41,41A) tabling (42,42A), has the peristome (63) that the inspection sample that passed through filtrator (46,46A) is passed through, at intermediate receptacle (42,42A) and should go up between the cubitainer (41,41A) cramping and keep going up side seal (44,44A) and following side seal (45,45A);
Lower reservoir (43,43A) is lower reservoir with portion that retains of checking sample of accommodating (80,80A) (43,43A), and this lower reservoir (43,43A) is to be maintained with respect to intermediate receptacle (42,42A) dismounting mode freely;
Intermediate receptacle (42,42A) has from peristome (63) and hangs down and have the guiding wall (64,64A) of lower opening portion (65) in the bottom;
This guiding wall (64,64A) is accommodated by the portion that retains that lower reservoir (43,43A) is had (80,80A), this retains portion (80,80A) and accepts the inspection sample of supplying with via filtrator (46,46A) from the lower opening portion (65) of guiding wall (64,64A), and, have the ventilating part (90) of the outside of the portion of retaining of leading to (80,80A) on this lower reservoir (43,43A) hole enlargement inclined-plane (92) above it.
11. the microplate of band filtrator as claimed in claim 10 is characterized in that, is provided with a plurality of ventilating parts (90).
12. the microplate of band filtrator as claimed in claim 11 is characterized in that, is provided with two ventilating parts (90).
13. the microplate as each described band filtrator in the claim 10 to 12 is characterized in that, the bottom of guiding wall (64,64A) hangs down into the degree of depth over half at least of the portion of retaining (80,80A).
14. the microplate as each described band filtrator in the claim 10 to 13 is characterized in that, auxiliary sealing member (47,47A) is installed between intermediate receptacle (42,42A) and lower reservoir (43,43A).
15. the microplate of band filtrator as claimed in claim 14 is characterized in that, auxiliary sealing member (47,47A) is shaped by unlike material in advance or embedded shaping is bonded on the lower reservoir (43,43A).
16. microplate as each described band filtrator in the claim 10 to 15, it is characterized in that, last cubitainer (41,41A) has the outer vertical wall of extending to vertical lower (52,52A) at peripheral part, and this outer vertical wall (52,52A) has the outstanding projection (53) towards foreign side;
Intermediate receptacle (42,42A) has to what vertical direction was extended at peripheral part and erects wall (60,60A), and this erects wall (60,60A) and has the outstanding projection (61) in side inwardly;
These projections (53,61) engagings, thus realize going up the chimeric of cubitainer (41,41A) and intermediate receptacle (42,42A).
17. microplate as each described band filtrator in the claim 10 to 16, it is characterized in that, last cubitainer (41,41A) has the embedded hole (56) that is made of upper section (57) and below part (58), upper section (57) has the hole enlargement hole, below part (58) constitute along with from the below towards the top and the hole of undergauge forms stage portion (59) between part (57) and the below part (58) up;
Intermediate receptacle (42,42A) has the stop pin (67) that extends towards the top, and this stop pin (67) has hole enlargement part (69) on top, and this hole enlargement part (69) has the interval (48) of extending to axis direction;
Be pressed into this stop pin (67) by below part (58) to embedded hole (56) from last cubitainer (41,41A), the hole enlargement part (69) of this stop pin (67) moves and undergauge to center position, by further being pressed into, stop pin (67) arrives upper section (57), this hole enlargement part (69) is engaged on the stage portion (59) of cubitainer (41,41A), thus, cubitainer in the realization (41,41A) and intermediate receptacle (42,42A) is chimeric.
18. microplate as each described band filtrator in the claim 10 to 17, it is characterized in that, filtrator (46,46A) is made by the etching and processing silicon chip, constitute by the central part of the through hole with a plurality of size homogeneous with from the peripheral part of this central part via this central part of encirclement of rake extension, peripheral part forms thicklyer than central part.
19. the microplate as each described band filtrator in the claim 10 to 18 is characterized in that each inscape is made of transparent material.
20. microplate as each described band filtrator in the claim 10 to 19, it is characterized in that, last cubitainer (41A) has flange rising portions (86) that extends upward from peripheral part and the opening rising portions (87) that extends upward continuously from peristome (50A), and these flange rising portions (86) and opening rising portions (87) extend to substantially the same height.
21. microplate as each described band filtrator in the claim 10 to 20, it is characterized in that, intermediate receptacle (42A) has from what peripheral part extended upward and erects wall (60A) and separate the inboard of both inboards of set a distance extending upward and erect wall (88) from erecting wall (60A) with this, erect wall (60A) and inboard at these and erect and embed the outer vertical wall (52A) that hangs down from last cubitainer (41A) in the space that limits between the wall (88), the height that the inboard erects wall (88) is lower than the height that erects wall (60A).
22. the microplate of band filtrator as claimed in claim 21 is characterized in that, the inboard erects the outer peripheral edges of side seal (44A) and following side seal (45A) in wall (88) maintenance.
23. the microplate as each described band filtrator in the claim 14 to 22 is characterized in that, intermediate receptacle (42A) has protuberance (89) on the face that joins with auxiliary sealing member (47A).
24. the microplate as each described band filtrator in the claim 1 to 23 is characterized in that, on the position of the adjacent openings portion of upper container (41) and/or bottom container (43), has the mark that is used for this each peristome position separately of specific expression.
25. microplate as each described band filtrator in the claim 1 to 24, it is characterized in that, on the side that joins with intermediate receptacle (42) of lower reservoir (43), has lower reservoir flank (30), rocking when this lower reservoir flank (30) is used to prevent from intermediate receptacle (42) and lower reservoir (43) are set.
26. microplate as each described band filtrator in the claim 1 to 25, it is characterized in that, in the end face complete cycle scope of the part that the periphery with auxiliary sealing member (47) of lower reservoir (43) is joined, has lower reservoir guide portion (35), rocking when this lower reservoir guide portion (35) is used to prevent from lower reservoir (43) and auxiliary sealing member (47) are set.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2006116668 | 2006-04-20 | ||
| JP116668/2006 | 2006-04-20 | ||
| JP322067/2006 | 2006-11-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101421627A true CN101421627A (en) | 2009-04-29 |
Family
ID=40631485
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007800137642A Pending CN101421627A (en) | 2006-04-20 | 2007-04-17 | Filter-carrying micro plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101421627A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014041488A1 (en) * | 2012-09-11 | 2014-03-20 | Centre Hospitalier Universitaire Vaudois | Conical multi-well filter plate |
| CN108730519A (en) * | 2018-07-25 | 2018-11-02 | 湖南省天骑医学新技术股份有限公司 | A kind of sealing rubber base and production method with negative pressure support platform |
| CN113039149A (en) * | 2018-11-09 | 2021-06-25 | 株式会社平山制作所 | Hole forming device and using method thereof |
-
2007
- 2007-04-17 CN CNA2007800137642A patent/CN101421627A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014041488A1 (en) * | 2012-09-11 | 2014-03-20 | Centre Hospitalier Universitaire Vaudois | Conical multi-well filter plate |
| CN108730519A (en) * | 2018-07-25 | 2018-11-02 | 湖南省天骑医学新技术股份有限公司 | A kind of sealing rubber base and production method with negative pressure support platform |
| CN108730519B (en) * | 2018-07-25 | 2023-09-29 | 湖南省天骑医学新技术股份有限公司 | Sealant seat with negative pressure supporting platform and manufacturing method |
| CN113039149A (en) * | 2018-11-09 | 2021-06-25 | 株式会社平山制作所 | Hole forming device and using method thereof |
| CN113039149B (en) * | 2018-11-09 | 2022-07-26 | 株式会社平山制作所 | Hole opening device and using method thereof |
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