CN217084993U - Inclined magnetic separation frame - Google Patents
Inclined magnetic separation frame Download PDFInfo
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- CN217084993U CN217084993U CN202123295662.0U CN202123295662U CN217084993U CN 217084993 U CN217084993 U CN 217084993U CN 202123295662 U CN202123295662 U CN 202123295662U CN 217084993 U CN217084993 U CN 217084993U
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Abstract
The utility model relates to an inclined magnetic separation frame, which comprises a magnet fixing frame, a magnet body and a sample tube positioning cambered surface clamp; magnet body slope setting, magnet mount are used for supporting the magnet body, and sample cell location cambered surface presss from both sides to be located the magnet body upside, and sample cell location cambered surface presss from both sides to be cylindrical, and sample cell location cambered surface presss from both sides to be used for holding the test tube, and sample cell location cambered surface presss from both sides to be 43-48 with the inclination of horizontal plane. Support the magnet body through the magnet mount for magnet body slope sets up, sample cell location cambered surface presss from both sides the upside that is located the magnet body, sample cell location cambered surface presss from both sides also slope setting, when the test tube that is equipped with the magnetism microballon is put sample cell location cambered surface and is pressed from both sides, can adsorb the magnetism microballon in the solution through the magnet body, thereby reach the purpose of magnetism microballon and solution separation, 43-48 setting makes the separation more thorough, magnetism microballon loss rate is low, the separation time is short.
Description
Technical Field
The utility model relates to a separation technology field in the thing medicine trade, concretely relates to tilting magnetic separation frame.
Background
The magnetic separation technology and the magnetic separation material are widely applied to a plurality of fields of in-vitro diagnosis, biological pharmacy, food sanitation inspection, environmental monitoring and the like.
1. The field of in vitro diagnostics.
In the field of in vitro diagnosis, the immunomagnetic microsphere is commonly used as a carrier of an immunologically active substance, the immunologically active substance such as an antigen and an antibody is connected to the immunomagnetic microsphere in a physical adsorption or chemical reaction mode to form an immunomagnetic microsphere-immunologically active substance compound, the compound can be fixed on the tube wall on a magnetic separation frame, and the redundant immunologically active substance can be removed by absorbing the solution, so that the purpose of separating the immunomagnetic microsphere-immunologically active substance compound from the solution is achieved. After the immune active substance is coated on the immune magnetic microsphere, the immune magnetic microsphere can be incubated with a sample, the immune active substance on the immune magnetic microsphere can be combined with a to-be-detected substance in the sample to form an immune magnetic microsphere-immune active substance-to-be-detected substance compound in the sample, the magnetic microsphere is adsorbed on the tube wall through the action force of a magnetic field, a solution is absorbed, the labeled immune active substance is added, and the immune magnetic microsphere-immune active substance-to-be-detected substance in the sample-labeled immune active substance is obtained through magnetic separation.
2. The field of biological pharmacy.
The targeting drug release system takes the immunomagnetic microspheres as a carrier, and carries the drug to a predetermined area under the action of an external magnetic field, so that the anticancer drug on the immunomagnetic microspheres can be more easily contacted with cancer cells, and the effect of killing the cancer cells is improved. In vitro purification of bone marrow by immunomagnetic microspheres has been widely used in autologous bone marrow transplantation following high-dose chemotherapy with encouraging results.
3. The field of food sanitation inspection.
Salmonella is one of the common food poisoning causing genera, and Skjerve et al reported that salmonella was isolated from milk and dairy products, meat and vegetables using immunomagnetic separation techniques with a detection limit of 102 bacteria per gram. Seo et al combined FIA with immunomagnetic separation and determined low concentrations of E.coli inoculated in beef, apple juice and raw milk, with only four E.coli per gram of beef being detectable.
4. The field of environmental monitoring.
Yu.H successfully applies immunomagnetic microspheres to quickly separate 2 x 10 in food-grade environmental water body -3 Coli0157 per mL and can complete the detection of 96 per h of sample. Sylvie et al have studied to enrich cryptophyceae-like basidiospores (a protozoa that can cause gastrointestinal diseases) in environmental water with immunomagnetic microspheres, and then detected by PCR amplification detection with sensitivity up to 1 oocyst per 100L of water.
In summary, magnetic separation technology plays an increasingly important role in human life in the present and future. However, the magnetic separation rack in the market still has the defects of incomplete separation, high loss rate of the magnetic microspheres and long separation time.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a magnetic separation frame of tilting to solve the separation thoroughly, magnetic microsphere loss rate height, the long problem of magnetic separation time.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
an inclined magnetic separation frame comprises a magnet fixing frame, a magnet body and a sample tube positioning cambered surface clamp; the magnet body slope sets up, the magnet mount is used for supporting the magnet body, sample cell location cambered surface presss from both sides and is located the magnet body upside, sample cell location cambered surface presss from both sides and is cylindrical, sample cell location cambered surface presss from both sides and is used for holding the test tube, the inclination that sample cell location cambered surface pressed from both sides and the horizontal plane is 43-48.
Further, the cross section of the magnet fixing frame is triangular.
Furthermore, a fixing groove is formed in the magnet fixing frame, the magnet body is located in the fixing groove, and the cross section of the magnet body is trapezoidal.
Further, the fixing groove is located at a position above the upper surface of the magnet fixing frame.
Furthermore, the lower end of the sample tube positioning arc surface clamp is fixedly connected with the lower end of the upper surface of the magnet fixing frame.
Furthermore, the magnetic induction intensity of the magnet body is 4000-.
Further, the diameter of the hole in the sample tube positioning cambered surface clamp is 10 +/-2 mm.
The utility model has the advantages that:
support the magnet body through the magnet mount for magnet body slope sets up, sample cell location cambered surface presss from both sides the upside that is located the magnet body, sample cell location cambered surface presss from both sides also slope setting, when the test tube that is equipped with the magnetism microballon is put sample cell location cambered surface and is pressed from both sides, can adsorb the magnetism microballon in the solution through the magnet body, thereby reach the purpose of magnetism microballon and solution separation, 43-48 setting makes the separation more thorough, magnetism microballon loss rate is low, the separation time is short.
Drawings
FIG. 1 is a schematic view of the overall structure of a tilted magnetic separation rack.
Names corresponding to the marks in the figure:
1-a magnet holder; 2-a magnet body; 3-sample tube positioning arc clamp.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
The embodiment of the utility model provides a:
referring to the attached figure 1, the inclined magnet fixing frame comprises a magnet fixing frame 1, a magnet body 2 and a sample tube positioning arc surface clamp 3; 2 slopes of magnet body set up, and magnet mount 1 is used for supporting magnet body 2, and sample cell location cambered surface presss from both sides 3 and is located 2 upsides of magnet body, and sample cell location cambered surface presss from both sides 3 and is cylindrical, and sample cell location cambered surface presss from both sides 3 and is used for holding the test tube.
The cross-section of 1 magnet mount is triangle-shaped, has seted up the fixed slot on the magnet mount 1, and the fixed slot is located the position that 1 upper surface of magnet mount leaned on, and magnet body 2 is located the fixed slot, and magnet body 2's cross-section is trapezoidal, and sample cell location cambered surface presss from both sides the lower extreme fixed connection of 3 and the lower extreme of 1 upper surface of magnet mount, and sample cell location cambered surface presss from both sides 3 upper surface that pastes tight magnet mount 1.
The magnetic induction intensity of the magnet body 2 is 4000-5000 gausses, and the magnet with the magnetic induction intensity can better adsorb the magnetic microspheres in the solution, so that the magnetic microsphere loss caused by too small magnetic force is avoided.
The diameter of the hole in the sample tube positioning cambered surface clamp 3 is 10 +/-2 mm. The inclination angle that sample cell location cambered surface pressed from both sides 3 and horizontal plane is 45, and when using, the bottom sprag face of magnet mount 1 is placed on the horizontal plane, so the slope contained angle that sample cell location cambered surface pressed from both sides 3 and the bottom sprag face of magnet mount 1 is 45.
Experiments were performed for 90 °, 60 °, 45 ° and 30 °, respectively, with 45 ° being the best setting.
The specific process of coating the SAA monoclonal antibody by using the magnet fixing frame 1 with the four sample tube positioning arc surface clamps 3 and the bottom supporting surface of the magnet fixing frame 1 respectively having the included angles of 90 degrees, 60 degrees, 45 degrees and 30 degrees is as follows:
step one, adopting four magnet fixing frames 1 with the included angles of 90 degrees, 60 degrees, 45 degrees and 30 degrees between the sample tube positioning arc surface clamp 3 and the bottom surface of the magnet fixing frame 1;
step two, coating immunomagnetic beads by the four magnet fixing frames 1 by the same process, and recording the magnetic separation time of each magnet fixing frame 1;
and step three, preparing a reagent by using the magnetic beads coated by the four magnet fixing frames 1, testing the same sample for 10 times by using the same parameters, comparing the average value of the test results of 10 times, and selecting the optimal included angle between the sample tube positioning arc surface clamp 3 and the bottom surface of the magnet fixing frame 1 by integrating other factors.
By comparing the four types of the magnet fixing frames 1 with the included angles of 90 degrees, 60 degrees, 45 degrees and 30 degrees between the sample tube positioning arc surface clamp 3 and the bottom surface of the magnet fixing frame 1, coating immunomagnetic beads with the sample tube positioning arc surface clamp and then preparing a reagent test sample, evaluating whether separation is thorough, the loss degree of the magnetic microspheres and the separation time, and obtaining the most appropriate included angle between the sample tube positioning arc surface clamp 3 and the bottom surface of the magnet fixing frame 1.
The results and the magnetic separation time of the magnetic bead test samples coated by the magnet fixing frame 1 with the included angles of 90 degrees, 60 degrees, 45 degrees and 30 degrees between the four sample tube positioning arc surface clamps 3 and the bottom surface of the magnet fixing frame 1 are shown in tables 1 and 2.
TABLE 1 test results of magnetic beads coated at included angles between positioning cambered surface clamp of different sample tubes and bottom surface of magnet fixing frame
TABLE 2 magnetic separation time of magnetic beads coated by included angles between positioning cambered surface clamps of different sample tubes and bottom surfaces of magnet fixing frames
In the embodiment, the optimal included angle between the sample tube positioning arc clamp 3 and the bottom surface of the magnet fixing frame 1 is obtained by comparing the four types of the magnet fixing frames 1 with the included angles of 90 degrees, 60 degrees, 45 degrees and 30 degrees between the sample tube positioning arc clamp 3 and the bottom surface of the magnet fixing frame 1, coating immunomagnetic beads with the sample tube positioning arc clamp, and then preparing a reagent test sample, and evaluating whether separation is complete, the loss degree of the magnetic microspheres and separation time. The result of the table 1 can obtain that the result of the test luminescence value of the magnetic bead coated by the magnet fixing frame 1 with the included angle of the sample tube positioning arc clamp 3 and the bottom surface of the magnet fixing frame 1 of 45 degrees is the highest, and the result of the table 2 can obtain that the magnetic separation time of the magnetic bead coated by the magnet fixing frame 1 with the included angle of the sample tube positioning arc clamp 3 and the bottom surface of the magnet fixing frame 1 of 45 degrees is the shortest, so that the included angle of the sample tube positioning arc clamp 3 and the bottom surface of the magnet fixing frame 1 is 45 degrees.
The present invention is not limited to the above-mentioned preferred embodiments, and any other products in various forms can be obtained by the teaching of the present invention, but any changes in the shape or structure thereof, which have the same or similar technical solutions as the present invention, fall within the protection scope of the present invention.
Claims (7)
1. An inclined magnetic separation frame is characterized by comprising a magnet fixing frame, a magnet body and a sample tube positioning cambered surface clamp; the magnet body slope sets up, the magnet mount is used for supporting the magnet body, sample cell location cambered surface presss from both sides and is located the magnet body upside, sample cell location cambered surface presss from both sides and is cylindrical, sample cell location cambered surface presss from both sides and is used for holding the test tube, the inclination that sample cell location cambered surface pressed from both sides and the horizontal plane is 43-48.
2. The canted magnetic separator bracket according to claim 1 wherein said magnet holder is triangular in cross-section.
3. The inclined magnetic separation rack of claim 1, wherein the magnet fixing rack is provided with a fixing groove, the magnet body is positioned in the fixing groove, and the cross section of the magnet body is trapezoidal.
4. The inclined magnetic separation rack of claim 3, wherein the fixing groove is located at a position above the upper surface of the magnet fixing rack.
5. The inclined magnetic separation rack of claim 4, wherein the lower end of the sample tube positioning arc clamp is fixedly connected with the lower end of the upper surface of the magnet fixing rack.
6. The inclined magnetic separation rack as claimed in claim 1, wherein the magnetic induction of the magnet body is 4000-5000 gauss.
7. The inclined magnetic separation rack of claim 1, wherein the diameter of the hole in the sample tube positioning arc clamp is 10 ± 2 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123295662.0U CN217084993U (en) | 2021-12-24 | 2021-12-24 | Inclined magnetic separation frame |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123295662.0U CN217084993U (en) | 2021-12-24 | 2021-12-24 | Inclined magnetic separation frame |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217084993U true CN217084993U (en) | 2022-07-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123295662.0U Active CN217084993U (en) | 2021-12-24 | 2021-12-24 | Inclined magnetic separation frame |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217084993U (en) |
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2021
- 2021-12-24 CN CN202123295662.0U patent/CN217084993U/en active Active
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