CN216998392U

CN216998392U - Stem cell culture device

Info

Publication number: CN216998392U
Application number: CN202220607437.1U
Authority: CN
Inventors: 杨光华; 王�华
Original assignee: SHANGHAI TELEBIO BIOMEDICAL CO Ltd
Current assignee: SHANGHAI TELEBIO BIOMEDICAL CO Ltd
Priority date: 2022-03-18
Filing date: 2022-03-18
Publication date: 2022-07-19
Anticipated expiration: 2032-03-18

Abstract

The utility model relates to the technical field of stem cell culture, in particular to a stem cell culture device, which comprises an incubator, wherein an operation cabinet is arranged in the incubator, a control chamber is arranged in the operation cabinet, a motor is fixed on the inner wall of the control chamber through a bolt, the power output end of the motor is connected with a driving shaft, and an operation dish is arranged at the top end of the driving shaft. According to the utility model, the culture box is arranged to form a closed space, so that external bacterial pollution is avoided, the operation cabinet is made of metal, the motor is started, the motor drives the driving shaft to rotate, the driving shaft drives the operation panel to rotate, the culture dish in the fixing groove is enabled to rotate and displace through the rotation of the operation panel, when a worker observes the culture dish through a microscope and a microscope lens, the culture dish can be rotated and displaced through the motor, the condition of multiple groups of cultured stem cells in the culture box can be seen, the operation is convenient, and the working efficiency is improved.

Description

Stem cell culture device

Technical Field

The utility model relates to the technical field of stem cell culture, in particular to a stem cell culture device.

Background

Stem cells are cells having self-replication function and multipotentiality, and can regenerate into various cells, tissues or organs of human body under specific conditions, and are called "universal cells" in medical science. The stem cells have important significance in basic research and transformation medical application, and have wide application prospects in the fields of regenerative medicine, disease models, drug screening, precise medicine and the like. However, since conventional stem cells are present in insufficient amounts, stem cell research has led to development in the field of induction of pluripotent stem cells, and attempts have been made to solve the problem of stem cells as a source of seed cells.

However, in the stem cell culture process, the culture device is too single, and the process of collecting and observing is difficult to adjust in the same environment, and the adjustment process needs to be carried out by opening the incubator, so that the operation is too complex, and the stem cell culture device is provided.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a stem cell culture device to propose the in-process to stem cell culture in solving above-mentioned background art, culture device is too single, is difficult to be in same environment and adjusts in the in-process of gathering and observing, and the adjustment process needs to open the incubator moreover and goes on, operates too loaded down with trivial details problem.

In order to achieve the above object, the present invention provides a stem cell culture apparatus, comprising: the incubator, the internally mounted of incubator has the operation cabinet, and the inside of operation cabinet is provided with the control room, the inner wall of control room has the motor through the bolt fastening, and the power take off end of motor is connected with the drive shaft, the top of drive shaft is provided with the operation panel, and the top of operation panel has seted up a plurality of fixed slots, settle the inside of fixed slot has the culture dish, top one side of incubator is provided with the microscope, and microscope's one end installs the microscope camera lens, the top opposite side of incubator inlays and is equipped with sealing glass, and sealing glass's outer wall has seted up the intercommunicating pore, the burette has been worn out to the inside of intercommunicating pore, and the top of burette is connected with the drip mouth.

Preferably, the incubator is fixedly connected with the operation cabinet through bolts, and the operation panel forms a rotating structure through a driving shaft and a motor.

Preferably, the fixed slots are uniformly distributed at equal intervals about the top end surface of the operating plate and are matched with the shape structure of the culture dish.

Preferably, the dropper penetrates through the incubator through the communication hole, and the dropper is communicated with the drip port.

Preferably, the sterilamp is installed to the inner wall of incubator, and the front end of incubator sets up the cover cuff, one side of control room is provided with puts the thing cabinet, and the opposite side arrangement of control room has the cabinet of accomodating, the bottom of incubator is provided with air cleaner, and the outer wall one side of incubator inlays and is equipped with the door plant.

Preferably, the incubator is detachably connected with the ultraviolet germicidal lamp through a bolt, and the incubator is fixedly connected with the air purifier through a bolt.

Preferably, the incubator is movably connected with the door plate through a hinge, and a closed structure is formed between the incubator and the door plate.

Compared with the prior art, the utility model has the beneficial effects that:

according to the utility model, the culture box is arranged to form a closed space, so that external bacterial pollution is avoided, the operation cabinet is made of metal, the motor is started, the motor drives the driving shaft to rotate, the driving shaft drives the operation plate to rotate, the culture dish in the fixed groove is enabled to rotate and displace through the rotation of the operation plate, when a worker observes the culture dish through the microscope and the microscope lens, the culture dish can be rotated and displaced through the motor, the condition of multiple groups of cultured stem cells in the culture box can be seen, the operation is convenient, and the working efficiency is improved.

According to the utility model, the drip openings are arranged in an elastic rubber structure, the drip openings and the drip pipes are combined into a fat belly drip pipe form, the lengthened drip pipes can accurately drip nutrient solution and the like into the culture dishes after entering the incubator through the communication holes, and the drip work can be carried out on different culture dishes through the work of the motor, so that the working efficiency is improved, the drip work is convenient and fast, the sealing glass can accurately view the condition in the incubator in a large area, and the preparation for the next process is facilitated.

According to the utility model, the door plate is connected with the incubator through the hinge, the incubator is opened and closed through the door plate, the ultraviolet germicidal lamp arranged on the inner wall of the incubator can be used for sterilizing, the inside of the incubator is in a sterile environment, the operation cabinet, the storage cabinet and the containing cabinet are integrated cabinet bodies, operation experiment tools and the like are placed in the storage cabinet, a worker can perform operation experiments on a culture dish through sleeves in sleeve openings, the containing cabinet is used for placing waste materials and to-be-cleaned devices, the door plate can be conveniently and uniformly replaced and cleaned, the opening and closing times of the door plate are reduced, the air purifier at the lowest part in the incubator can quickly replace and purify air after the experiment is finished or when gas needs to be replaced, and the incubator has the comprehensiveness and the functionality.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic front view of the present invention;

FIG. 3 is a schematic top view of the present invention;

FIG. 4 is a schematic view of the structure of the operation cabinet of the present invention;

in the figure: 1. an incubator; 2. an operation cabinet; 3. a control room; 4. a motor; 5. a drive shaft; 6. an operation panel; 7. fixing grooves; 8. a culture dish; 9. a microscope; 10. a microscope lens; 11. sealing the glass; 12. a communicating hole; 13. a drip port; 14. a dropper; 15. an ultraviolet germicidal lamp; 16. sleeve openings; 17. a storage cabinet; 18. a storage cabinet; 19. an air cleaner; 20. a door panel is provided.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-4, a stem cell culture apparatus includes: the incubator 1, an operation cabinet 2 is installed inside the incubator 1, a control chamber 3 is arranged inside the operation cabinet 2, a motor 4 is fixed on the inner wall of the control chamber 3 through bolts, a driving shaft 5 is connected to the power output end of the motor 4, an operation panel 6 is arranged on the top end of the driving shaft 5, a plurality of fixing grooves 7 are formed in the top end of the operation panel 6, a culture dish 8 is arranged inside the fixing grooves 7, a microscope 9 is arranged on one side of the top end of the incubator 1, a microscope lens 10 is installed at one end of the microscope 9, the incubator 1 is fixedly connected with the operation cabinet 2 through bolts, the operation panel 6 forms a rotating structure with the motor 4 through the driving shaft 5, the fixing grooves 7 are uniformly distributed at equal intervals relative to the top end of the operation panel 6, the fixing grooves 7 are matched with the appearance structure of the culture dish 8, the incubator 1 is arranged to form a closed space, and external bacterial pollution is avoided, the operation cabinet 2 is made of metal, the motor 4 is started, the driving shaft 5 is driven to rotate by the work of the motor 4, the operation disc 6 is driven to rotate by the rotation of the driving shaft 5, the culture dish 8 in the fixed groove 7 is enabled to rotate and displace through the rotation of the operation disc 6, when a worker observes the culture dish 8 through the microscope 9 and the microscope lens 10, the culture dish 8 can be rotated and displaced through the motor 4, the condition of multiple groups of cultured stem cells in the incubator 1 can be seen, the operation is convenient, the working efficiency is improved, the sealing glass 11 is embedded on the other side of the top end of the incubator 1, the communication hole 12 is formed in the outer wall of the sealing glass 11, the dropper 14 penetrates through the inside of the communication hole 12, the dropper 13 is connected to the top end of the dropper 14, the dropper 14 penetrates through the inside of the incubator 1 through the communication hole 12, the dropper 14 is mutually communicated with the drip hole 13, and the drip hole 13 is of an elastic rubber structure, drip mouth 13 and burette 14 make up into fat tripe burette form, elongated burette 14 passes through the intercommunicating pore 12 and gets into incubator 1 back, can be more accurate instil into culture dish 8 inside nutrient solution etc. through the work of motor 4, can carry out the dropping liquid work to different culture dishes 8, improve work efficiency, let dropping liquid work become convenient and fast, the condition of incubator 1 inside is watched to the large tracts of land that sealing glass 11 can be more accurate, be convenient for prepare for next step's process.

Referring to fig. 2 and 4, a stem cell culture apparatus includes: an ultraviolet sterilization lamp 15 is arranged on the inner wall of an incubator 1, a sleeve opening 16 is arranged at the front end of the incubator 1, a storage cabinet 17 is arranged on one side of a control room 3, a storage cabinet 18 is arranged on the other side of the control room 3, an air purifier 19 is arranged at the bottom of the incubator 1, a door panel 20 is embedded on one side of the outer wall of the incubator 1, the incubator 1 is detachably connected with the ultraviolet sterilization lamp 15 through bolts, the incubator 1 is fixedly connected with the air purifier 19 through bolts, the incubator 1 is movably connected with the door panel 20 through hinges, a closed structure is formed between the incubator 1 and the door panel 20, the door panel 20 is connected with the incubator 1 through hinges, the incubator 1 is closed and opened through the door panel 20, the ultraviolet sterilization lamp 15 arranged on the inner wall of the incubator 1 can be sterilized, the interior of the incubator 1 is in a sterile environment, the operation cabinet 2, the storage cabinet 17 and the storage cabinet 18 are an integrated cabinet body, put 17 inside operations experiment tools etc. of having placed of thing cabinet, the staff can operate experiment etc. to culture dish 8 through 16 inside oversleeves of cuff, accomodate cabinet 18 and place the waste material and wait to wash the utensil, be convenient for change the clearance through door plant 20 is unified, reduce door plant 20's the closed number of times of switch, incubator 1 inside below air purifier 19, after the experiment cultivation finishes, or when needing to change gas, change the purification to the air fast, time culture apparatus more has comprehensive and functional.

The working principle is as follows: before using the culture device, firstly, the door plate 20 is connected with the culture box 1 through a hinge, so that the culture box 1 and the door plate 20 are combined into a switchable closed type sealed box body, the culture box 1 is opened, a culture dish 8 for storing stem cells is placed on a fixed groove 7, the fixed groove 7 is matched with the appearance structure of the culture dish 8 and is tightly attached, the fixed groove 7 is equidistantly distributed on the upper end of an operation plate 6, nine groups are arranged, so that simultaneous multi-group culture and observation are convenient, an ultraviolet sterilizing lamp 15 is opened, the culture box 1 is sterilized, the interior of the culture box 1 is in a sterile environment, the top end of the culture box 1 is provided with a microscope 9, a microscope lens 10 at the bottom end of the microscope 9 penetrates through the culture box 1 and is positioned above the operation plate 6, so that the stem cells in the culture dish 8 can be observed, the operation cabinet 2 is arranged below the operation plate 6, the interior of the operation cabinet 2 is provided with a control chamber 3, the inside motor 4 through the bolt fastening of control room 3, through starter motor 4, the work of motor 4 drives drive shaft 5 and rotates, drive shaft 5 drives operation panel 6 and rotates, culture dish 8 rotates through operation panel 6, through the work of motor 4, microscope 9 can observe different groups of culture dishes 8, the top opposite side of incubator 1 inlays dress sealing glass 11, the inside concrete condition of observation between being convenient for, burette 14 and drip mouth 13 make up into fat tripe burette, burette 14 runs through sealing glass 11 through intercommunicating pore 12, be in culture dish 8 top, increase nutrient solution etc. to culture dish 8 inside through fat tripe burette, avoid opening the interpolation and let incubator 1 get into a large amount of bacteriums, operation cabinet 2, put thing cabinet 17 and containing cabinet 18 formula cabinet body as an organic whole, place the required apparatus of cultivateing the experiment inside operation cabinet 2, through the aseptic oversleeve that 16 covers of oversleeves, the staff directly cultivates operations such as experiment through aseptic oversleeve in incubator 1, avoid often taking the influence and cultivate the result, the equipment facility and the waste material that need change are placed and are being accomodate cabinet 18, open incubator 1 through door plant 20 and change in unison and take, reduce the switch number of times, and 2 belows in the operation cabinet are provided with air cleaner 19, and at the cultivation in-process or after finishing, need carry out the air and change and start the use when purifying, let culture apparatus more have the functionality.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A stem cell culture device, comprising:

the incubator (1), an operation cabinet (2) is installed inside the incubator (1), a control chamber (3) is arranged inside the operation cabinet (2), a motor (4) is fixed on the inner wall of the control chamber (3) through bolts, a driving shaft (5) is connected with the power output end of the motor (4), an operation disc (6) is arranged at the top end of the driving shaft (5), a plurality of fixing grooves (7) are formed in the top end of the operation disc (6), a culture dish (8) is arranged inside the fixing grooves (7), a microscope (9) is arranged on one side of the top end of the incubator (1), a microscope lens (10) is arranged at one end of the microscope (9), sealing glass (11) is embedded on the other side of the top end of the incubator (1), a communication hole (12) is formed in the outer wall of the sealing glass (11), a dropper (14) penetrates out of the inside of the communication hole (12), and the top end of the dropper (14) is connected with a dropping liquid port (13).

2. The stem cell culture device according to claim 1, wherein the incubator (1) is fixedly connected with the operation cabinet (2) through bolts, and the operation panel (6) forms a rotating structure with the motor (4) through the driving shaft (5).

3. The stem cell culture device according to claim 1, wherein the fixing grooves (7) are uniformly distributed at equal intervals with respect to the top end surface of the operating plate (6), and the fixing grooves (7) are matched with the shape of the culture dish (8).

4. The stem cell culture apparatus according to claim 1, wherein the dropper (14) is inserted into the incubator (1) through the communication hole (12), and the dropper (14) and the drip port (13) communicate with each other.

5. The stem cell culture device according to claim 1, wherein an ultraviolet germicidal lamp (15) is installed on the inner wall of the incubator (1), a sleeve opening (16) is formed at the front end of the incubator (1), a storage cabinet (17) is arranged on one side of the control room (3), a storage cabinet (18) is arranged on the other side of the control room (3), an air purifier (19) is arranged at the bottom of the incubator (1), and a door panel (20) is embedded on one side of the outer wall of the incubator (1).

6. The stem cell culture device according to claim 5, wherein the incubator (1) is detachably connected to the UV germicidal lamp (15) by bolts, and the incubator (1) is fixedly connected to the air purifier (19) by bolts.

7. The stem cell culture device according to claim 5, wherein the incubator (1) is movably connected with the door panel (20) through a hinge, and a closed structure is formed between the incubator (1) and the door panel (20).