CN216900569U - Structure and immunoblotting appearance are held in carrying of immunoblotting membrane - Google Patents
Structure and immunoblotting appearance are held in carrying of immunoblotting membrane Download PDFInfo
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- CN216900569U CN216900569U CN202123229609.0U CN202123229609U CN216900569U CN 216900569 U CN216900569 U CN 216900569U CN 202123229609 U CN202123229609 U CN 202123229609U CN 216900569 U CN216900569 U CN 216900569U
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- Prior art keywords
- membrane
- immunoblotting
- plate
- adhesive tape
- waterproof adhesive
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- 239000012528 membrane Substances 0.000 title claims abstract description 83
- 238000003119 immunoblot Methods 0.000 title claims abstract description 64
- 239000002390 adhesive tape Substances 0.000 claims abstract description 28
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 18
- 238000004140 cleaning Methods 0.000 claims abstract description 18
- 238000012546 transfer Methods 0.000 claims description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 10
- 238000000034 method Methods 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 6
- 239000012085 test solution Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 4
- 239000000853 adhesive Substances 0.000 description 3
- 230000001070 adhesive effect Effects 0.000 description 3
- 230000003028 elevating effect Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002998 immunogenetic effect Effects 0.000 description 1
- 230000036046 immunoreaction Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
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- 239000007790 solid phase Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Abstract
The utility model provides a carrying structure of an immunoblotting membrane and an immunoblotting instrument. The carrying structure of the immunoblotting membrane comprises an attached plate and a waterproof adhesive tape, wherein the attached plate is vertically arranged, the back surface of the immunoblotting membrane leans against the surface of the attached plate, and the waterproof adhesive tape is adhered between the immunoblotting membrane and the attached plate. By adopting the technical scheme of the utility model, the attachment plate provides an attachment plane for the immunoblotting membrane, so that the position of the immunoblotting membrane is kept stable in the transferring process; the top of the immunoblotting membrane is fixed directly through the waterproof adhesive tape, so that the method is convenient and rapid, and the experimental efficiency is improved; the waterproof adhesive tape has small regulation effect on the floating of the immunoblotting membrane in a reagent or a cleaning solution, is beneficial to the full reaction of the blotting membrane and the reagent or the cleaning solution, and improves the accuracy of an experimental result.
Description
Technical Field
The utility model relates to a biochemical experiment auxiliary device, in particular to a carrying structure of an immunoblotting membrane and an immunoblotting instrument.
Background
The immunoblot assay is also called Western Blot assay (Western Blot). The method is one common experiment method in biochemistry and immunogenetics, and includes transferring total cell or tissue protein after electrophoretic separation from gel to solid carrier NC membrane or PVDF membrane, immunoreacting with corresponding antibody with the protein or polypeptide in the solid carrier as antigen, reacting with enzyme or isotope labeled second antibody, and final substrate color development or autoradiography to detect the protein component expressed by specific target gene after electrophoretic separation. In the whole immunoreaction experiment, the solid phase carrier membrane needs to be subjected to washing, incubation and washing operations of multiple test solutions and multiple frequencies, the time consumption is long, the operation frequency is high, and the working strength of experimenters is high.
The small-size immunoblotting analysis appearance that adopts in the laboratory at present generally is to place the sample in the reaction vessel, and in the experimentation, add the test solution into the reaction vessel, through the suction gun suction test solution and wash after single reaction finishes again, the antibody that contains a large amount of unconsumed in giving up anti test solution and two anti test solutions has caused the serious waste of anti and two antibodies, and the price of anti and two antibodies is comparatively expensive, has leaded to higher experimental cost.
In chinese patent document No. CN208999442U, the present applicant proposes a sample membrane clamping mechanism and an immunoblotting apparatus, which stably clamp a sample membrane through a loading membrane sheet and a pressing membrane sheet, so as to facilitate rapid transfer of the sample membrane and realization of active oscillation of the sample membrane in an experimental process, thereby improving experimental efficiency and saving the usage amount of a test solution. However, since the above-mentioned clamping operation of the sample membrane requires a certain amount of time, how to increase the speed of clamping the immunoblotting membrane and further improve the efficiency of the experiment has become a new problem to be studied by the present applicant.
SUMMERY OF THE UTILITY MODEL
In order to overcome the defects of the prior art, the utility model aims to provide a carrying structure of an immunoblotting membrane and an immunoblotting instrument.
In order to achieve the purpose, the technical scheme adopted by the utility model for solving the technical problems is as follows: the carrying structure of the immunoblotting membrane comprises an attached plate and a waterproof adhesive tape, wherein the attached plate is vertically arranged, the back surface of the immunoblotting membrane leans against the surface of the attached plate, and the waterproof adhesive tape is adhered between the immunoblotting membrane and the attached plate.
By adopting the technical scheme of the utility model, the attachment plate provides an attachment plane for the immunoblotting membrane, so that the position of the immunoblotting membrane is kept stable in the transferring process; the top of the immunoblotting membrane is fixed directly through the waterproof adhesive tape, so that the method is convenient and rapid, and the experimental efficiency is improved; the waterproof adhesive tape has small restriction on the floating of the immunoblotting membrane in a reagent or cleaning solution, is beneficial to the full reaction of the blotting membrane and the reagent or the cleaning solution, and improves the accuracy of an experimental result.
Furthermore, one end of the waterproof adhesive tape is adhered to the attached plate, and the other end of the waterproof adhesive tape is adhered to the outer surface of the upper part of the immunoblotting membrane.
By adopting the preferable scheme, the adhesive tape is convenient to paste, and stable adhesive force between the waterproof adhesive tape and the attached plate and between the waterproof adhesive tape and the immunoblotting membrane is ensured.
Further, the waterproof adhesive tape is one or more.
Further, when the waterproof adhesive tape is in a plurality of strips, a space is formed between every two adjacent waterproof adhesive tapes.
By adopting the preferable scheme, a reasonable number of fixing points are selected according to the type of the immunoblotting membrane, and the stability of the immunoblotting membrane is improved.
Furthermore, an indicating line is arranged on the auxiliary plate.
With the preferred scheme, the position of the blotting membrane on the attached plate is controlled according to the indication line.
An immunoblotter comprising:
a reagent tank;
a cleaning tank;
the above-described support structure for an immunoblotting membrane;
and the transfer mechanism is used for driving the attached plate to move between the reagent tank and the cleaning tank.
The immunoblotting apparatus employs the above-described structure for supporting an immunoblotting membrane, and therefore, has all the advantageous effects of the above-described structure for supporting an immunoblotting membrane.
Furthermore, the transfer mechanism comprises a rotating mechanism and a lifting mechanism, the reagent tank and the cleaning tank are distributed along the circumference, the lifting mechanism drives the auxiliary plate to move up and down, and the rotating mechanism drives the lifting mechanism to rotate.
By adopting the preferable scheme, the reagent tank and the cleaning tank are distributed along the circumference, so that the structure is simplified, the space is saved, and the laboratory configuration is more convenient.
Furthermore, a membrane plate frame is installed on the lifting block of the lifting mechanism, and the auxiliary plate is detachably installed on the membrane plate frame.
Furthermore, the top of the accessory plate is provided with a carrying part, and the accessory plate is inserted into the slot of the membrane plate frame.
Adopt above-mentioned preferred scheme, can take off with attaching the board earlier, after the fixed immunoblotting membrane, insert the board of will attaching again and establish on the diaphragm plate frame, made things convenient for experimental operation.
Further, the number of the membrane plate frames is multiple, and the multiple membrane plate frames are distributed along the circumference array.
By adopting the preferable scheme, the single membrane loading capacity of the immunoblotting instrument is improved, and the experimental efficiency is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a structural view of one embodiment of a support structure of an immunoblotting membrane of the present invention;
FIG. 2 is a block diagram of one embodiment of an immunoblotter of the present invention;
figure 3 is a cross-sectional view of one embodiment of an immunoblotter of the present invention.
Names of corresponding parts represented by numerals and letters in the drawings:
11-attaching a plate; 111-indicator line; 112-a carrying part; 12-waterproof adhesive tape; 13-immunoblotting membrane; 21-reagent tank; 22-a cleaning tank; 31-a rotation mechanism; 32-a lifting mechanism; 33-membrane plate frame.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1, the immunoblotting membrane holding structure includes an attachment plate 11 and a waterproof tape 12, the attachment plate 11 is vertically disposed, the back surface of the immunoblotting membrane 13 is attached to the surface of the attachment plate 11, and the waterproof tape 12 is adhered between the immunoblotting membrane 13 and the attachment plate 11.
The beneficial effect of adopting above-mentioned technical scheme is: the attaching plate provides an attaching plane for the immunoblotting membrane, so that the position of the immunoblotting membrane is kept stable in the transferring process; the top of the immunoblotting membrane is fixed directly through the waterproof adhesive tape, so that the method is convenient and rapid, and the experimental efficiency is improved; the waterproof adhesive tape has small regulation effect on the floating of the immunoblotting membrane in a reagent or a cleaning solution, is beneficial to the full reaction of the blotting membrane and the reagent or the cleaning solution, and improves the accuracy of an experimental result.
In other embodiments of the present invention, waterproof tape 12 is adhered to attachment plate 11 at one end and to the upper outer surface of immunoblotting membrane 13 at the other end. The beneficial effect of adopting above-mentioned technical scheme is: not only is convenient for sticking the adhesive, but also ensures the stable adhesive force between the waterproof adhesive tape and the accessory plate as well as between the waterproof adhesive tape and the immunoblotting membrane.
In other embodiments of the present invention, the waterproof tape is one or more strips. When the waterproof adhesive tape is a plurality of waterproof adhesive tapes, a space is reserved between every two adjacent waterproof adhesive tapes. The beneficial effect of adopting above-mentioned technical scheme is: according to the type of the immunoblotting membrane, a reasonable number of fixed points are selected to improve the stability of the immunoblotting membrane.
In other embodiments of the present invention, as shown in fig. 1, an indicator line 111 is provided on the attachment plate 11. The beneficial effect of adopting above-mentioned technical scheme is: the position of the blotting membrane on the attached plate is controlled according to the indication line.
As shown in fig. 2, an immunoblotter comprising:
a reagent tank 21;
a cleaning tank 22;
the above-mentioned support structure for an immunoblotting membrane;
and a transfer mechanism for moving the attachment plate 11 between the reagent tank 21 and the cleaning tank 22.
As shown in fig. 3, in another embodiment of the present invention, the transferring mechanism includes a rotating mechanism 31 and an elevating mechanism 32, the reagent tank 21 and the cleaning tank 22 are distributed along the circumference, the elevating mechanism 32 drives the accessory plate 11 to move up and down, and the rotating mechanism 31 drives the elevating mechanism 32 to rotate. The specific form of the rotating mechanism 31 is not limited and can be selected from the prior art, such as a form in which a motor drives a pulley via a drive belt. The specific form of the lifting mechanism 32 is not limited, and can be selected from the prior art, such as a motor driving a lead screw to rotate, and then matching with a vertical guide rail. The beneficial effect of adopting above-mentioned technical scheme is: the reagent tank and the cleaning tank are distributed along the circumference, so that the structure is simplified, the space is saved, and the laboratory configuration is more convenient.
As shown in fig. 1 to 3, in other embodiments of the present invention, the lifting block of the lifting mechanism 32 is mounted with a membrane plate holder 33, and the accessory plate 11 is detachably mounted on the membrane plate holder 33. The top of the accessory plate 11 is provided with a holding part 112, and the accessory plate 11 is inserted into the slot of the film plate frame 33. The beneficial effect of adopting above-mentioned technical scheme is: the auxiliary plate can be taken down firstly, and after the immunoblotting membrane is fixed, the auxiliary plate is inserted into the membrane plate frame, so that the experimental operation is facilitated.
In other embodiments of the present invention, the number of membrane plate holders 33 is multiple, and the multiple membrane plate holders are distributed along a circumferential array. The beneficial effect of adopting above-mentioned technical scheme is: the single membrane loading capacity of the immunoblotting instrument is improved, and the experimental efficiency is improved.
The above-mentioned embodiments are only for illustrating the technical idea and features of the present invention, and the purpose of the present invention is to enable those skilled in the art to understand the content of the present invention and implement the present invention, and not to limit the protection scope of the present invention, and all equivalent changes or modifications made according to the spirit of the present invention should be covered in the protection scope of the present invention.
Claims (8)
1. The carrying structure of the immunoblotting membrane is characterized by comprising an attached plate and a waterproof adhesive tape, wherein the attached plate is vertically arranged, the back surface of the immunoblotting membrane leans against the surface of the attached plate, and the waterproof adhesive tape is adhered between the immunoblotting membrane and the attached plate; one end of the waterproof adhesive tape is adhered to the attached plate, and the other end of the waterproof adhesive tape is adhered to the outer surface of the upper part of the immunoblotting membrane; and an indicating line is arranged on the auxiliary plate.
2. The carrier structure for an immunoblotting membrane according to claim 1, wherein said waterproof tape is one or more strips.
3. The carrier structure for an immunoblotting membrane according to claim 2, wherein when the waterproof tape is a plurality of tapes, a space is provided between adjacent waterproof tapes.
4. An immunoblotter, comprising:
a reagent tank;
a cleaning tank;
a support structure for an immunoblotting membrane according to any of claims 1-3;
and the transfer mechanism is used for driving the attached plate to move between the reagent tank and the cleaning tank.
5. The immunoblotting instrument of claim 4 wherein said transfer mechanism comprises a rotating mechanism and a lifting mechanism, said reagent tank and said cleaning tank are circumferentially distributed, said lifting mechanism drives said attachment plate to move up and down, said rotating mechanism drives said lifting mechanism to rotate.
6. The immunoblotting instrument of claim 5 wherein said elevator block of said elevator mechanism has a membrane plate holder mounted thereon, and said accessory plate is removably mounted on said membrane plate holder.
7. The immunoblotting instrument of claim 6 wherein a holding portion is provided at the top of the accessory plate, and the accessory plate is inserted into the slot of the membrane plate holder.
8. The immunoblotter of claim 6 wherein the membrane panel mount is in plurality and the plurality of membrane panel mounts are distributed in a circumferential array.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123229609.0U CN216900569U (en) | 2021-12-21 | 2021-12-21 | Structure and immunoblotting appearance are held in carrying of immunoblotting membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123229609.0U CN216900569U (en) | 2021-12-21 | 2021-12-21 | Structure and immunoblotting appearance are held in carrying of immunoblotting membrane |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216900569U true CN216900569U (en) | 2022-07-05 |
Family
ID=82207929
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123229609.0U Expired - Fee Related CN216900569U (en) | 2021-12-21 | 2021-12-21 | Structure and immunoblotting appearance are held in carrying of immunoblotting membrane |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216900569U (en) |
-
2021
- 2021-12-21 CN CN202123229609.0U patent/CN216900569U/en not_active Expired - Fee Related
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220705 |
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| CF01 | Termination of patent right due to non-payment of annual fee |