CN114814192A

CN114814192A - Novel immunology detect reagent box

Info

Publication number: CN114814192A
Application number: CN202210277208.2A
Authority: CN
Inventors: 刘晓华; 王勇; 王小娜
Original assignee: Changle County Peoples Hospital
Current assignee: Changle County Peoples Hospital
Priority date: 2022-03-21
Filing date: 2022-03-21
Publication date: 2022-07-29

Abstract

The invention provides a novel immunological detection kit, and relates to the technical field of immunological detection. This novel immunological detect reagent box, including the kit body, the kit body is the light-resistant box body, the lid is gone up to kit body upper end fixedly connected with, it is the light-resistant lid to go up the lid, the fixed buffering support limit that is provided with of bottom avris in the kit body, the fixed reaction cup support frame that is provided with of buffering support edge, reaction cup support frame lower extreme is provided with places the bottom plate, it is fixed to be provided with the fixing base in bottom plate upper end to place, the fixing base both ends are all fixed to be provided with and carry the end. The invention provides a novel immunological detection kit, which has the advantages of fewer required instruments, simple and easy operation, capability of being used for quantitative and qualitative detection of sample solution in batches quickly, accurately and quickly, and can obtain detection results of a plurality of indexes within a few hours by adding a sample once, low requirement on operators and particularly suitability for basic level use.

Description

Novel immunology detect reagent box

Technical Field

The invention relates to the technical field of immunological detection, in particular to a novel immunological detection kit.

Background

ELISA kits are called by a number of names in China, for example: ELISA detection Kit, ELISA adsorption assay Kit, ELISA analysis Kit, enzyme immunoassay Kit, etc., the more common names are ELISA detection Kit, ELISA adsorption assay Kit, etc., since the 60-70 s, ELISA Kit has been produced, has been recognized and advocated by researchers all over the world, the Elisa biological test is an experimental diagnosis method with high sensitivity, strong specificity and good repeatability, which is greatly popularized in Europe, America and China, particularly the long-term development of the domestic biochemical field, because of the factors of stable reagent, easy storage, simple operation, objective result judgment and the like, the method has been widely applied to various fields of immunological tests, the ELISA detection kit is suitable for in vitro qualitative detection of human Hepatitis E (HEV) virus IgM antibody in human serum or plasma by ELISA.

At present, immunological detection needs a large amount of detection kits for immunological detection, but the traditional immunological detection kits are simpler in structure, inconvenient to use and carry, easily cause damage to the internal detection reagent bottles, further influence the immunological detection, influence the detection result and use inconveniently.

The prior patent (application number is CN 201420336227.9) provides an immunological detection kit for tetracycline, which comprises a box body, a box cover, a bottom fixing seat and a top fixing seat, wherein the bottom fixing seat and the top fixing seat are arranged in the box body, various reagent bottle concave positions are arranged on the bottom fixing seat and are used for placing tetracycline antibody working solution, enzyme-labeled secondary antibody working solution, developing solution, stop solution, concentrated washing solution, tetracycline standard solution, matrix diluent and Tween-20 standby solution, various clamping grooves or grooves are arranged on the top fixing seat and are used for placing an ELISA plate, a micropore strip, a straw, a colorimetric card and pH test paper, the kit adopts an indirect competitive ELISA method to carry out qualitative and quantitative detection on tetracycline content, and the kit has the advantages of simple operation, high sensitivity, low cost and the like, and is suitable for field rapid detection of large-batch samples. However, the structure of this kit is comparatively simple, can not carry out effectual protection to inside reagent bottle, and the condition that reagent bottle damaged appears easily in the transportation to the kit, causes the damage of kit, influences the normal use of kit to this kit can not realize carrying out porous application of sample with the rifle of arranging simultaneously, the time difference of application of sample can appear, thereby reduces the accuracy of testing result.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a novel immunological detection kit, which solves the problems that the traditional immunological detection kit is simple in structure, inconvenient to use and carry, and easy to damage an internal detection reagent bottle, thereby influencing the immunological detection.

(II) technical scheme

In order to realize the purpose, the invention is realized by the following technical scheme: the utility model provides a novel immunology detect reagent box, includes the kit body, the kit body is the light-resistant box body, kit body upper end fixedly connected with goes up the lid, it is the light-resistant lid to go up the lid, the fixed buffering support limit that is provided with of bottom avris in the kit body, the fixed reaction cup support frame that is provided with of buffering support edge, reaction cup support frame lower extreme is provided with places the bottom plate, place the fixed fixing base that is provided with in bottom plate upper end, the fixing base both ends are all fixed to be provided with and carry the end, the inside a plurality of draw-in grooves of placing that are provided with of fixing base, it is a plurality of place the inside fixed porous reaction cup that is provided with of draw-in groove, the fixed joint board that is provided with in porous reaction cup both ends all.

Preferably, two jacks are fixedly arranged on the lower side of the front end of the kit body.

Preferably, two sheet plugs are fixedly arranged on the lower side of the front end of the upper box cover and are respectively inserted into the two jacks.

Preferably, a label is fixedly arranged at the front end of the upper box cover.

Preferably, pull grooves are fixedly formed in the middle of the two lifting end heads.

Preferably, the buffering support edge is made of soft rubber.

Preferably, the first placing seat and the second placing seat are both made of plastic foam, and a plurality of clamping grooves are formed in the upper end of the first placing seat and the second placing seat.

Preferably, the detection reagent bottle is a reagent bottle containing an antibody or a reagent bottle containing a color developing agent.

Preferably, the auxiliary reagent bottle is a plurality of reagent bottles such as a standard product bottle, a termination liquid bottle, a concentrated washing liquid bottle, a concentrated diluent liquid bottle, a substrate buffer liquid bottle and the like, the concentrated washing liquid bottle contains concentrated washing liquid, and the concentrated diluent liquid bottle contains concentrated protein buffer liquid.

The working principle is as follows: when the novel immunology detection kit is used, the insertion holes are arranged on the kit body, the sheet plug is arranged on the upper box cover, the kit is conveniently sealed and the sealing operation is more convenient, the kit is more convenient to unseal, a porous reaction cup and a reaction cup support frame are arranged at the upper end of the buffer support frame, certain buffer can be performed, the reaction cup support frame can not cause rigid collision in the transportation process, the porous reaction cup is effectively protected, the reaction cup is safer to place, a placing seat made of foam plastic is arranged at the bottom of the kit, a detection reagent bottle and an auxiliary reagent bottle are clamped in the clamping groove at the upper end of the placing seat, the buffer support frame matched with the side edge plays a double buffer protection role, and the detection reagent bottle and the auxiliary reagent bottle are effectively protected, the reagent bottle and the auxiliary reagent bottle are safer to use, the detection process is carried out in the porous reaction cups, when samples to be detected with different concentrations or standard products or reagents diluted by times are added into the porous reaction cups, the multi-hole sample adding can be carried out by using the arranging guns through the multi-hole reaction cups arranged in parallel, the time difference of the sample adding is effectively eliminated, and the accuracy of the detection result is effectively improved.

(III) advantageous effects

The invention provides a novel immunological detection kit. The method has the following beneficial effects:

1. the invention provides a novel immunology detection kit, which is convenient for capping the kit by arranging the jack on the kit body and arranging the sheet plug on the upper box cover, is more convenient for capping operation, is more convenient for unsealing the kit, and is more convenient for use.

2. The invention provides a novel immunological detection kit, wherein a buffer supporting edge is arranged in the kit, and a porous reaction cup and a reaction cup supporting frame are arranged at the upper end of the buffer supporting edge, so that certain buffer can be performed, the reaction cup supporting frame cannot cause rigid collision in the transportation process, the porous reaction cup is effectively protected, the reaction cup is placed more safely, a placing seat made of foam plastic is arranged at the bottom of the kit, a detection reagent bottle and an auxiliary reagent bottle are clamped in a clamping groove at the upper end of the placing seat, and the buffer supporting edge at the side is matched to play a double buffer protection role, so that the detection reagent bottle and the auxiliary reagent bottle are effectively protected, and the detection reagent bottle and the auxiliary reagent bottle are used more safely.

3. The invention provides a novel immunological detection kit, wherein the detection process is carried out in a porous reaction cup, when samples to be detected with different concentrations or standard products or reagents diluted by multiple ratios are added into the porous reaction cup, the multi-hole sample adding can be simultaneously carried out by using a discharging gun through the multi-hole reaction cups arranged in parallel, the time difference of the sample adding is effectively eliminated, and the accuracy of the detection result is effectively improved.

4. The invention provides a novel immunological detection kit, which has the advantages of fewer required instruments, simple and easy operation, capability of being quickly, accurately and qualitatively detected in batch of sample solution, capability of obtaining detection results of a plurality of indexes within a few hours by adding a sample once, low requirement on operators and particular suitability for basic level use.

Drawings

FIG. 1 is a perspective view of the present invention;

FIG. 2 is a schematic view of the internal structure of the present invention;

FIG. 3 is a top view of the cartridge body of the present invention;

FIG. 4 is an expanded view of the present invention;

FIG. 5 is a schematic view of the structure of the reaction cup holder of the present invention;

FIG. 6 is a schematic view showing the placement of the multi-well reaction cup of the present invention.

Wherein, 1, the kit body; 2. an upper box cover; 3. a label; 4. a jack; 5. a sheet plug; 6. a reaction cup support frame; 7. a buffer support edge; 8. a first placing seat; 9. a second placing seat; 10. detecting a reagent bottle; 11. an auxiliary reagent bottle; 12. placing a bottom plate; 13. a fixed seat; 14. pulling the end head; 15. groove drawing; 16. placing a clamping groove; 17. a clamping and connecting plate; 18. a porous reaction cup.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.

Example 1:

as shown in fig. 1 to 6, an embodiment of the present invention provides a novel immunology detection kit, which includes a kit body 1, wherein the kit body 1 is a light-shielding box body, an upper box cover 2 is fixedly connected to an upper end of the kit body 1, the upper box cover 2 is a light-shielding box cover, a buffering support edge 7 is fixedly disposed on a bottom side in the kit body 1, a reaction cup support frame 6 is fixedly disposed on an upper end of the buffering support edge 7, a placement bottom plate 12 is disposed on a lower end of the reaction cup support frame 6, a fixing base 13 is fixedly disposed on an upper end of the placement bottom plate 12, lifting end heads 14 are fixedly disposed at both ends of the fixing base 13, a plurality of placement slots 16 are disposed inside the fixing base 13, a plurality of porous reaction cups 18 are fixedly disposed inside the placement slots 16, and clamping plates 17 are fixedly disposed at both ends of the porous reaction cups 18.

Two jacks 4 are fixedly arranged on the lower side of the front end of a kit body 1, two sheet plugs 5 are fixedly arranged on the lower side of the front end of an upper box cover 2, the two sheet plugs 5 are respectively inserted into the two jacks 4, a label 3 is fixedly arranged on the front end of the upper box cover 2, pull grooves 15 are fixedly arranged in the middle of two pull ends 14, a buffer supporting edge 7 is made of soft rubber, a first placing seat 8 and a second placing seat 9 are made of plastic foam, a plurality of clamping grooves are formed in the upper end of the first placing seat, a detection reagent bottle 10 is a reagent bottle containing an antibody or a reagent bottle containing a color developing agent, an auxiliary reagent bottle 11 is a standard product bottle, a termination liquid bottle, a concentrated washing liquid bottle, a concentrated diluting liquid bottle, a substrate buffering liquid bottle and other various reagent bottles, the concentrated washing liquid bottle contains concentrated washing liquid, and the concentrated diluting liquid bottle contains concentrated protein buffer liquid.

The operation steps of using the kit are as follows:

1. opening an upper box cover of the kit, taking out the reaction cup support frame, and taking out the detection reagent bottle and the auxiliary reagent bottle which need to be used;

2. numbering sample reagent bottles and standard sample reagent bottles corresponding to the porous reaction cups in sequence, making 2 holes for each sample and each standard sample in parallel, and recording positions of the standard holes and the sample holes;

3. adding 50 mul of standard substance or sample into a corresponding porous reaction cup, adding the detection reagent in the detection reagent bottle, slightly vibrating and uniformly mixing, adding the auxiliary reagent in the auxiliary reagent bottle if necessary, covering a plate with a cover plate film, and reacting for a period of time in a dark environment at 37 ℃;

4. then, the results are judged according to the reaction conditions in the porous reaction cups.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A novel immunological detection kit comprises a kit body (1), and is characterized in that: the reagent kit comprises a reagent kit body (1), wherein an upper box cover (2) is fixedly connected to the upper end of the reagent kit body (1), a buffering supporting edge (7) is fixedly arranged on the side of the inner bottom of the reagent kit body (1), a reaction cup supporting frame (6) is fixedly arranged on the upper end of the buffering supporting edge (7), a placing bottom plate (12) is arranged at the lower end of the reaction cup supporting frame (6), a fixing seat (13) is fixedly arranged on the upper end of the placing bottom plate (12), lifting end heads (14) are fixedly arranged at the two ends of the fixing seat (13), a plurality of placing clamping grooves (16) are arranged in the fixing seat (13), a plurality of porous reaction cups (18) are fixedly arranged in the placing clamping grooves (16), and clamping plates (17) are fixedly arranged at the two ends of the porous reaction cups (18).

2. The novel immunological detection kit according to claim 1, wherein: two jacks (4) are fixedly arranged on the lower side of the front end of the kit body (1).

3. The novel immunological detection kit according to claim 1, wherein: two sheet plugs (5) are fixedly arranged on the lower side of the front end of the upper box cover (2), and the two sheet plugs (5) are respectively inserted into the two jacks (4).

4. The novel immunological detection kit according to claim 1, wherein: and a label (3) is fixedly arranged at the front end of the upper box cover (2).

5. The novel immunological detection kit according to claim 1, wherein: pulling grooves (15) are fixedly arranged in the middle of the two pulling ends (14).

6. The novel immunological detection kit according to claim 1, wherein: the buffer supporting edge (7) is made of soft rubber.

7. The novel immunological detection kit according to claim 1, wherein: the first placing seat (8) and the second placing seat (9) are both made of plastic foam, and a plurality of clamping grooves are formed in the upper end of the first placing seat and the second placing seat.

8. The novel immunological detection kit according to claim 1, wherein: the detection reagent bottle (10) is a reagent bottle containing an antibody or a reagent bottle containing a color developing agent.

9. The novel immunological detection kit according to claim 1, wherein: the auxiliary reagent bottle (11) is a plurality of reagent bottles such as a standard product bottle, a termination liquid bottle, a concentrated washing liquid bottle, a concentrated dilution liquid bottle, a substrate buffer liquid bottle and the like.