CN216838003U

CN216838003U - Three gas RTP incubator

Info

Publication number: CN216838003U
Application number: CN202122388130.5U
Authority: CN
Inventors: 陈滨阳; 张铭; 胥坤
Original assignee: Shanghai Tofflon Medical Equipment Co ltd
Current assignee: Shanghai Tofflon Medical Equipment Co ltd
Priority date: 2021-09-29
Filing date: 2021-09-29
Publication date: 2022-06-28
Anticipated expiration: 2031-09-29

Abstract

The utility model discloses a three-gas RTP incubator, which comprises a shell, an inner cavity, a gas distribution plate and an electric box, wherein one side of the shell is provided with a front cover, and the front cover is provided with an RTP auxiliary valve for butting with a main valve; heating wires, temperature and humidity sensors and CO are distributed in the inner cavity₂Concentration sensor, O₂Concentration sensor, H₂O₂Concentration sensor and turbulent fan. The utility model discloses a principle of space docking ware, on using the incubator with RTP, realize with the quick butt joint of aseptic isolator, realize carrying out the mutual supply of internal resources under airtight aseptic environment, satisfied the requirement that the space was kept apart in the cell medicine production, the inside accessible VHP of cavity sterilizes, and the bactericidal effect is good, guarantees the inside sterility can requirement of cavity, accords with the standard requirement of cGMP.

Description

Three gas RTP incubator

Technical Field

The utility model relates to a three gas RTP incubators belongs to aseptic medicine transfer technical field.

Background

The production of cell drugs must be carried out in a sterile environment, and all production operations also need sterility assurance, and finally, the cell drugs are safely returned to patients. The incubator is an important component of an aseptic production environment (provides a culture environment), the inner cavity of the three-gas incubator on the market cannot meet the requirement of closed sterility, and the pollution of a sample by an external gas source or the environment in the culture process is difficult to control or reduce; in addition, the common three-gas incubator is not provided with an integrated sterile operation instrument and equipment interface, so that the requirement on sterility is difficult to meet in the operation processes of cell liquid exchange, observation, harvesting and the like; and the process data parameter of the incubator is not recorded, so that the process problem tracking is difficult to realize.

SUMMERY OF THE UTILITY MODEL

The utility model discloses the technical problem that will solve is: the existing three-gas incubator has the problem that the sterility is difficult to control in the operation processes of cell liquid changing, observation, harvesting and the like.

In order to solve the technical problem, the utility model provides a three gas RTP incubators, it includes:

the device comprises a shell, a front cover is arranged on one side of the shell, and an RTP auxiliary valve used for being in butt joint with a main valve is arranged on the front cover;

an inner cavity arranged in the shell, heating wires distributed on the outer wall of the inner cavity, a temperature and humidity sensor and CO₂Concentration sensor, O₂Concentration sensor, H₂O₂A concentration sensor and a turbulent fan;

gas distribution plate, O₂Pipeline, N₂Pipeline, CO₂The pipeline and the VHP/Air pipeline respectively pass through O₂Pipeline solenoid valve, N₂Pipeline solenoid valve, CO₂The pipeline electromagnetic valve and the VHP/Air pipeline electromagnetic valve are connected with a gas distribution plate and CO₂The pipeline is connected with CO through a gas distribution plate₂Pipe shut-off valve, O₂Pipeline, N₂The pipelines are combined into O through a gas distribution plate₂/N₂Pipeline back connection O₂/N₂Pipeline shut-off valve, CO₂Pipeline shut-off valve, O₂/N₂The pipeline shut-off valve is communicated with the inside of the inner cavity; the VHP/Air pipeline passes through the gas distribution plate and then is connected with CO₂Pipeline shut-off valve, O₂/N₂The pipeline between the pipeline shut-off valve and the inner cavity is respectively communicated; the inner part of the inner cavity is also communicated with an Outlet pipeline, and the Outlet pipeline is connected with an Outlet pipeline electromagnetic valve through an air distribution disc;

the electric box is internally provided with a starting button for one-key starting and a control unit for controlling each electric element.

Preferably, the CO is₂CO is arranged between the pipeline shut-off valve and the inner cavity₂And (4) a pipeline filter.

Preferably, said O is₂/N₂O is arranged between the pipeline shut-off valve and the inner cavity₂/N₂And (4) a pipeline filter.

Preferably, an Outlet pipeline filter is arranged on an Outlet pipeline between the inner cavity and the gas distribution disc.

Preferably, a front cover door catch is arranged on one side of the front cover and used for locking the front cover. Maintain incubator protecgulum temperature through protecgulum auxiliary heating to avoid incubator qianmen to appear condensation phenomenon.

Preferably, the bottom of the shell is provided with casters for supporting and moving the tri-gas RTP incubator to slide on the honeycomb rails, thereby facilitating sample transfer.

Preferably, the control unit is connected with the computer by adopting a data wireless communication (WIFI) technology, and can maintain data after being separated, so that data management and tracking are facilitated.

Said O is₂The concentration sensor is used for monitoring the concentration value of the gas in the inner cavity and feeding back the concentration value to the control unit, and the control unit performs logical operation according to the fed-back value and controls CO₂Pipeline solenoid valve, N₂Pipeline solenoid valve and O₂The pipeline electromagnetic valve is switched on and off to adjust the gas concentration value in the inner cavity; the temperature and humidity sensor is used for monitoring the temperature and humidity inside the box body, the temperature value is fed back to the control unit, and the control unit controls the heating wire to be switched on and off to maintain the temperature of the inner cavity to be set after logic operation is carried out according to the fed back value.

The utility model discloses a gas jacket formula heating methods, the temperature recovery is fast, and temperature homogeneity is good. The utility model discloses well RTP sub-valve realizes the aseptic quick transfer of sample with the main valve butt joint, avoids the risk of sample pollution in the transfer process. Each air source main pipeline adopts a sterilization and filtration unit, and the external air source can be controlled to pollute the inside of the three-air RTP culture box; the principle of the space docking device is adopted, RTP (rapid transport interface) is applied to the incubator, rapid docking with the sterile isolator is realized, mutual supply of internal resources is realized in a closed sterile environment, and the requirement of space isolation in cell drug production is met. If the system is realized by a plurality of cases, a plurality of incubators are operated simultaneously, the culture system can realize the culture of a plurality of patients in the same room in a D + A environment, each incubator is independent and has no problem of intercrossed pollution, the culture system integrates the common function of the tightness test, and the leakage rate detection can be conveniently carried out on the current sealing performance of the incubators in real time; the system has the common function of VHP (steam peroxide sterilization), the sterilization effect can reach 6 logs, the requirement of sterile performance in the cavity is ensured, and the cGMP standard requirement is met.

Compared with the prior art, the beneficial effects of the utility model are that: the design of a full-closed culture system is adopted, the risk of pollution or cross contamination is low, the VHP sterilization effect is good, and the cGMP standard requirement is met; can realize accurate oxygen concentration and CO₂Controlling the gas concentration; and data integrity management is convenient for data tracking. The utility model provides a when cell anaerobic culture under present non-sterile environment, the contaminated risk of sample.

Drawings

FIG. 1 is a front view of a three-gas RTP incubator provided by the present invention;

FIG. 2 is a side view of FIG. 1;

fig. 3 is a schematic diagram of the internal pipeline of the three-gas RTP incubator provided by the present invention.

Detailed Description

In order to make the present invention more comprehensible, preferred embodiments are described in detail below with reference to the accompanying drawings.

Examples

As shown in fig. 1-3, for the utility model provides a three gas RTP incubator, it includes:

the valve comprises a shell 10, wherein a front cover 20 is arranged on one side of the shell 10, and an RTP auxiliary valve 21 for butting with a main valve is arranged on the front cover 20; a front cover door latch 22 is provided at one side of the front cover 20 for locking the front cover. The temperature of the front cover of the incubator is maintained by the auxiliary heating of the front cover, so that the condensation phenomenon of the front door of the incubator is avoided;

an inner cavity 40 arranged in the shell 10, heating wires 46 distributed in the inner cavity 40, a temperature and humidity sensor 41 and CO₂Concentration sensor 43, O₂Concentration sensor 44, H₂O₂ A concentration sensor 45 and a disturbing flow fan 42;

an electrical box 30, wherein a start button 31 for one-key start and a control unit 32 for controlling each electrical element are arranged in the electrical box 30; the control unit 32 is connected with the computer by adopting a data wireless communication (WIFI) technology, and can maintain data after being separated, so that data management and tracking are facilitated;

gas distribution plate 50, O₂Pipeline, N₂Pipeline, CO₂The pipeline and the VHP/Air pipeline respectively pass through O₂ Pipeline solenoid valves 511, N₂ Pipeline solenoid valve 512, CO₂The pipeline electromagnetic valve 513 and the VHP/Air pipeline electromagnetic valve 514 are connected with the gas distribution plate 50, CO₂The pipeline is connected with CO through a gas distribution plate 50₂Pipeline shut-off valves 517, O₂Pipeline, N₂The pipelines pass through the gas distribution plate 50 and are combined into O₂/N₂Pipeline back connection O₂/N₂Pipeline shut-off valve 518, CO₂Pipeline shut-off valves 517, O₂/N₂A line shut-off valve 518 communicates with the interior of the inner chamber 40; VHP/Air pipeline passes through gas distribution plate 50 and then is in contact with CO₂Pipeline shut-off valves 517, O₂/N₂The pipeline between the pipeline cut-off valve 518 and the inner cavity 40 is respectively communicated; the interior of the inner cavity 40 is also in communication with an Outlet line, which is connected to an Outlet line solenoid valve 515 via a gas panel 50.

The CO is₂CO is arranged between the pipeline cut-off valve 517 and the inner cavity 40₂ Line filter 519, VHP/Air line access CO₂PipelineShut-off valve 517 and CO₂Between in-line filters 519.

Said O is₂/N₂O is arranged between the pipeline cut-off valve 518 and the inner cavity 40₂/N₂Pipeline filter 520, VHP/Air pipeline access O₂/N₂Line shutoff valve 518 and O₂/N₂Between in-line filters 520.

An Outlet pipeline filter 516 is arranged on the Outlet pipeline between the inner cavity 40 and the gas distribution plate 50.

The bottom of the shell 10 is provided with casters 11 for supporting and moving the three-gas RTP incubator to slide on the honeycomb rails, thereby facilitating the transfer of the sample.

Said O is₂The concentration sensor 44 is used for monitoring the concentration value of the gas in the inner cavity and feeding back the concentration value to the control unit 32, and the control unit 32 performs logical operation according to the feedback value and then controls CO₂ Pipeline solenoid valves 513, N₂ Line solenoid valves 512 and O₂The pipeline electromagnetic valve 511 is switched on and off to adjust the gas concentration value in the inner cavity 40; the temperature and humidity sensor 41 is configured to monitor the temperature and humidity inside the box, and feed back the temperature value to the control unit 32, and the control unit 32 performs logical operation according to the fed back value and then controls the power on and off of the heating wire 46 to maintain the inner cavity 40 at the set temperature.

In the VHP sterilization injection stage of the inner cavity 40, the RTP incubator bee nest culture system sends steam peroxide gas to the VHP/Air pipeline, and the control unit 32 maintains the concentration of the peroxide gas, CO, in the cavity by controlling the on-off of the electromagnetic valve 514 of the VHP/Air pipeline₂Pipeline shut-off valves 517, O₂/N₂The line shutoff valve 518 needs to be closed and the exhaust port solenoid valve 515 is opened; in the VHP sterilization and residue removal stage of the inner cavity 40, the RTP incubator bee nest culture system sends dry and clean Air to the VHP/Air pipeline, and the control unit 32 reduces the concentration of the gas in the cavity by controlling the opening of the electromagnetic valve 514 of the VHP/Air pipeline; CO 2₂Pipeline shut-off valves 517, O₂/N₂The line shutoff valve 518 needs to be closed, the exhaust port solenoid valve 515 is opened, and the interior H of the chamber₂O₂Concentration value through H₂O₂Concentration sensor45 real-time on-line monitoring.

The utility model relates to a three gas RTP incubators contain conventional 460RTP valve, 350RTP valve, 270RTP valve, 190RTP valve and 105RTP valves etc. standard RTP valve all are applicable.

Claims

1. A three gas RTP incubator, comprising:

the device comprises a shell (10), wherein a front cover (20) is arranged on one side of the shell (10), and an RTP auxiliary valve (21) which is used for being in butt joint with a main valve is arranged on the front cover (20);

an inner cavity (40) arranged in the shell (10), a heating wire (46) distributed on the outer wall of the inner cavity (40), a temperature and humidity sensor (41) and CO₂Concentration sensor (43), O₂Concentration sensor (44), H₂O₂A concentration sensor (45) and a turbulent fan (42);

gas distribution plate (50), O₂Pipeline, N₂Pipeline, CO₂The pipeline and the VHP/Air pipeline respectively pass through O₂Pipeline solenoid valve (511), N₂Pipeline solenoid valve (512), CO₂The pipeline electromagnetic valve (513) and the VHP/Air pipeline electromagnetic valve (514) are connected with a gas distribution plate (50), and CO₂The pipeline is connected with CO through a gas distribution plate (50)₂Pipeline shut-off valve (517), O₂Pipeline, N₂The pipelines are merged into O through a gas distribution disc (50)₂/N₂Pipeline back connection O₂/N₂Pipeline shut-off valve (518), CO₂Pipeline shut-off valve (517), O₂/N₂A line shut-off valve (518) in communication with the interior of the inner chamber (40); the VHP/Air pipeline passes through a gas distribution plate (50) and then is connected with CO₂Pipeline shut-off valve (517), O₂/N₂The pipeline between the pipeline shut-off valve (518) and the inner cavity (40) is respectively communicated; the inner part of the inner cavity (40) is also communicated with an Outlet pipeline, and the Outlet pipeline is connected with an Outlet pipeline electromagnetic valve (515) through a gas distribution disc (50);

the electric box (30), the electric box (30) is equipped with the start button (31) that is used for a key start-up and is used for controlling the control unit (32) of each electric component.

2. The tri-gas RTP incubator of claim 1 wherein the CO is₂CO is arranged between the pipeline shut-off valve (517) and the inner cavity (40)₂An inline filter (519).

3. The trioxygen RTP incubator of claim 1, wherein the O is₂/N₂O is arranged between the pipeline shut-off valve (518) and the inner cavity (40)₂/N₂An in-line filter (520).

4. A three-gas RTP incubator as claimed in claim 1, wherein an Outlet line filter (516) is provided on the Outlet line between the inner chamber (40) and the gas panel (50).

5. A trioxygen RTP incubator as claimed in claim 1, wherein one side of the front cover (20) is provided with a front cover door latch (22).

6. A trioxygen RTP incubator as claimed in claim 1, characterized in that the bottom of the enclosure (10) is provided with castors (11).

7. A trioxypane RTP incubator as claimed in claim 1, characterized in that the control unit (32) is connected to a computer using data wireless communication technology.