CN216738339U - Collection mechanism for chip capture cells - Google Patents

Abstract

The utility model relates to a collection mechanism of cell is caught to chip, it is including being used for setting up the collection seat of placing the seat and being used for setting up the butt joint subassembly at the movable plate, it is used for placing micro-fluidic chip to place the seat, it is provided with the collecting pipe that can get and put to collect the seat, the butt joint subassembly is used for the play appearance hole butt joint intercommunication with micro-fluidic chip, the mouth of pipe of collecting pipe corresponds the setting with the mouth of pipe of the play appearance pipe of butt joint subassembly. The application has the effect of improving the efficiency of blood sample collection in the microfluidic chip.

Description

Collection mechanism for chip capture cells

Technical Field

The application relates to the field of cell capture equipment, in particular to a collecting mechanism for capturing cells on a chip.

Background

The cell capturing and dyeing instrument is based on a micro-fluidic chip technology, realizes the enrichment of rare cells by combining a micro-fluidic control and immune recognition mode, realizes the automatic unattended capturing and analysis of the rare cells in clinical blood samples by various sample introduction systems and cell capturing and recognizing through the cell capturing and dyeing instrument, and can be applied to the fields of accurate noninvasive prenatal screening/diagnosis (NIPT), early tumor screening and diagnosis and the like.

The cell capturing and staining instrument performs blood sample injection capturing and staining on a microfluidic chip in a communication device through a connecting device in butt joint with the injection pump mechanism, and realizes detection and analysis on the microfluidic chip. When the blood sample captured and stained in the microfluidic chip needs to be further analyzed and detected, the blood sample in the microfluidic chip needs to be taken out, but the blood sample captured in the microfluidic chip is usually taken out manually, so that the method is complicated.

SUMMERY OF THE UTILITY MODEL

In order to improve the efficiency of blood sample collection in a microfluidic chip, the application provides a collection mechanism for capturing cells by the chip.

The collection mechanism for the chip capture cells adopts the following technical scheme:

the utility model provides a collection mechanism of cell is caught to chip, is including being used for setting up at the collection seat of placing the seat and being used for setting up the butt joint subassembly at the movable plate, place the seat and be used for placing micro-fluidic chip, the collection seat is provided with the collecting pipe that can get and put, the butt joint subassembly is used for the play appearance hole butt joint intercommunication with micro-fluidic chip, the mouth of pipe of collecting pipe corresponds the setting with the mouth of pipe of the play appearance pipe of butt joint subassembly.

By adopting the technical scheme, after the injection pump mechanism injects the blood sample into the microfluidic chip to complete cell capture, the captured blood sample is guided into the collection tube to be collected through the docking assembly in docking communication with the sample outlet hole of the microfluidic chip, so that the blood sample collection efficiency in the microfluidic chip is improved.

Preferably, the docking assembly is located below the placing seat, the collecting docking joint of the docking assembly is used for being in docking communication with the sample outlet hole of the microfluidic chip, and the sample outlet pipe of the docking assembly is located above the collecting pipe.

Through adopting above-mentioned technical scheme, through the collection butt joint on the butt joint subassembly and the play appearance hole butt joint intercommunication of micro-fluidic chip, the blood sample that goes out the appearance pipe outflow falls to collecting in the collector tube and collects.

Preferably, the butt joint subassembly still includes the butt joint board, collect the butt joint and go out the appearance pipe and all be connected with the butt joint board, and collect the butt joint and go out the appearance pipe and communicate each other through the butt joint board, butt joint board fixed connection is on the movable plate, just collect the butt joint and pass and extend to the movable plate top, the movable plate shifts up through the push-up subassembly to the messenger collects the butt joint and communicates with the butt joint of the appearance hole that goes out of micro-fluidic chip.

By adopting the technical scheme, the moving plate is pushed to move upwards by the push-up assembly, so that the collection butt joint on the butt joint plate is in butt joint communication with the sample outlet hole of the micro-fluidic chip, and the butt joint of the collection butt joint and the micro-fluidic chip is conveniently realized.

Preferably, be provided with the waste liquid head on the butt joint plate, be provided with the three-way valve on the butt joint plate, the inlet and the collection butt joint intercommunication of three-way valve, one of them liquid outlet and the drain pipe intercommunication of three-way valve, another liquid outlet and the waste liquid head intercommunication of three-way valve.

Through adopting above-mentioned technical scheme, gate collection butt joint and waste liquid head through the three-way valve, carry out blood sample collection or waste liquid and collect the discharge.

Preferably, place the seat and be used for the interval to place a plurality of micro-fluidic chips, it sets up a plurality ofly to collect butt joint and three-way valve, and is a plurality of to collect butt joint and three-way valve interval fixed the setting on the buttcover plate, and every collects the butt joint respectively with the play sample hole intercommunication of the micro-fluidic chip that corresponds, the inside intercommunication passageway that is provided with of buttcover plate, the liquid outlet intercommunication that intercommunication passageway and a plurality of three-way valves correspond, the play liquid end and the waste liquid head intercommunication of intercommunication passageway.

Through adopting above-mentioned technical scheme, a plurality of butt joints of collecting realize collecting the blood sample or the waste liquid of a plurality of micro-fluidic chip to improve the collection efficiency of blood sample and waste liquid.

Preferably, a waste liquid receiving hopper is arranged below the waste liquid head.

Through adopting above-mentioned technical scheme, accept the fill through the waste liquid and conveniently accept the waste liquid and carry out centralized processing.

Preferably, the waste liquid receiving hopper is obliquely arranged towards one side, and a waste liquid drainage joint is arranged on the side wall of the waste liquid receiving hopper positioned on the oblique side.

Through adopting above-mentioned technical scheme, the waste liquid that sets up to one side slope holds the fill and connects the discharge collection from waste liquid flowing back joint with the waste liquid of collecting.

Preferably, the collecting seat is provided with a placing groove, and the collecting pipe is placed in the placing groove.

Through adopting above-mentioned technical scheme, the collecting pipe passes through the standing groove convenience.

In summary, the present application includes at least one of the following beneficial technical effects:

1. after the injection pump mechanism injects the blood sample into the microfluidic chip to complete cell capture, the captured blood sample is guided into the collection tube for collection through the docking assembly in docking communication with the sample outlet hole of the microfluidic chip, so that the efficiency of blood sample collection in the microfluidic chip is improved;

2. the collecting butt joint and the waste liquid head are gated through a three-way valve, and blood samples are collected or waste liquid is collected and discharged;

3. the blood sample or the waste liquid of a plurality of micro-fluidic chips are collected through a plurality of collecting butt joints, so that the collection efficiency of the blood sample and the waste liquid is improved.

Drawings

FIG. 1 is a schematic diagram showing the overall structure of a cell-capturing staining apparatus according to an embodiment of the present invention.

Fig. 2 is a schematic structural diagram of a communication device in an embodiment of the present application.

Fig. 3 is an exploded view of the communication device in the embodiment of the present application.

Fig. 4 is a schematic structural diagram of a docking assembly in an embodiment of the present application.

Fig. 5 is a schematic view of the installation structure of the push-up assembly in the frame in the embodiment of the present application.

FIG. 6 is a front view of a docking assembly in an embodiment of the present application.

FIG. 7 is a sectional view of A-A in the example of the present application.

FIG. 8 is a schematic view of the installation structure of the waste liquid receiving hopper on the communication device in the embodiment of the present application.

Description of reference numerals: 1. a housing; 2. a communication device; 3. a microfluidic chip; 4. a frame; 41. a base plate; 411. a window; 42. a top plate; 421. a viewing port; 43. a first motor; 44. a first lead screw; 45. a first linear guide rail; 46. moving the plate; 461. sampling and butting joints; 47. a vertical plate; 48. a second linear guide; 49. a connecting plate; 5. a placing seat; 51. a containing groove; 52. a slice taking groove; 53. mounting blocks; 54. a let position port; 6. a collection mechanism; 61. a collecting seat; 611. sinking a step; 612. a placement groove; 613. a collection pipe; 62. a docking assembly; 621. a butt plate; 6211. a waste liquid head; 6212. a three-way valve; 6213. a communication channel; 622. collecting the butt joint; 6221. a seal ring; 623. a sample outlet pipe; 63. a push-up assembly; 631. a second motor; 632. a second screw rod; 633. a cross arm; 6331. a first arm; 6332. a second arm; 6333. a first lug; 6334. a pushing block; 6335. a second lug; 6336. a moving block; 7. a waste liquid receiving hopper; 71. waste liquid drainage connects.

Detailed Description

The present application is described in further detail below with reference to figures 1-8.

The embodiment of the application discloses a collecting mechanism for chip capture cells. Referring to fig. 1, the cell capturing and staining instrument includes a housing 1 and a communication device 2 disposed on the housing 1, the communication device 2 is used for placing a microfluidic chip 3, and the communication device 2 injects a blood sample into the microfluidic chip 3 for cell capturing through a syringe pump mechanism in the housing 1.

Referring to fig. 2, the communicating means 2 includes a frame 4 and a placing seat 5, the placing seat 5 is slidably connected to the frame 4, and the frame 4 is provided with a driving mechanism capable of driving the placing seat 5 to slide out of or into the frame 4. The frame 4 includes a bottom plate 41 and a top plate 42, two sides of the lower surface of the top plate 42 are protruded downward and are fixedly connected with the bottom plate 41 through screws, so as to form a space for accommodating the placing seat 5, and the upper surface of the placing seat 5 is provided with a plurality of accommodating grooves 51 for accommodating the microfluidic chip 3. In this embodiment, four accommodating grooves 51 are provided, and the four accommodating grooves 51 are spaced along the upper surface of the placing base 5. The upper surface of the placing seat 5 at both sides of the containing groove 51 is provided with a tablet taking groove 52 which is concave downwards. The upper surface of the top plate 42 is provided with an observation port 421 corresponding to the containing groove 51, and the microfluidic chip 3 is positioned in the containing groove 51 stably by the limitation of the lower surface of the top plate 42. The collecting mechanism 6 comprises a collecting seat 61 and a docking assembly 62, the collecting seat 61 is fixedly installed at the end of the placing seat 5, the placing seat 5 is provided with a downward sinking step 611 downwards, a plurality of placing grooves 612 are formed in the position of the sinking step 611, and collecting pipes 613 are placed on the placing grooves 612.

Referring to fig. 3, the driving mechanism includes a first motor 43 and a first lead screw 44, the first motor 43 is installed at one side of the frame 4, the first lead screw 44 is coaxially and fixedly arranged with an output shaft of the first motor 43, and the first lead screw 44 is in threaded connection with the placing base 5. The placing seat 5 is connected with the frame 4 through a first linear guide rail 45 in a sliding manner, and the first linear guide rail 45 is arranged in parallel with the first screw rod 44. The number of the first linear guide rails 45 is two, the two first linear guide rails 45 are respectively arranged on the bottom plate 41 positioned at two sides of the placing seat 5, the two sides of the placing seat 5 are both outwards integrally provided with the mounting blocks 53, the first linear guide rails 45 are fixedly mounted on the bottom plate 41, and the first sliding blocks of the first linear guide rails 45 are fixedly mounted on the lower surfaces of the mounting blocks 53.

Referring to fig. 3 and 4, the frame 4 is movably provided with a moving plate 46, the moving plate 46 is located below the placing seat 5, the moving plate 46 is provided with a sample outlet butt joint 461 corresponding to the microfluidic chip 3, and the bottom plate 41 is provided with a window 411 through which the moving plate 46 moves. The docking assembly 62 comprises a docking plate 621, a collecting docking head 622 and a sample outlet tube 623, the docking plate 621 is fixedly mounted on the lower surface of the moving plate 46, the collecting docking head 622 is fixedly connected to the upper surface of the docking plate 621 and extends above the moving plate 46, and the collecting docking head 622 and the sample outlet tube 623 are communicated with each other through the docking plate 621. The sample outlet pipe 623 is screwed on the side wall of the docking plate 621, and a pipe orifice of the sample outlet pipe 623 extends to a position above a pipe orifice of the collecting pipe 613, so that the pipe orifice of the collecting pipe 613 and the pipe orifice of the sample outlet pipe 623 of the docking assembly 62 are correspondingly arranged. The frame 4 is provided with a push-up assembly 63 capable of driving the moving plate 46 to drive the sample outlet butt joint 461 and the collection butt joint 622 to be in butt joint communication with the sample inlet hole and the sample outlet hole of the microfluidic chip 3 in the frame 4. The groove bottom of the containing groove 51 is provided with a yielding port 54 communicated with the outside, the sample outlet butt joint 461 and the collecting butt joint 622 penetrate through the yielding port 54 through the push-up component 63 to be butted with a sample inlet hole and a sample outlet hole of the microfluidic chip 3, and the sample outlet butt joint 461 and the collecting butt joint 622 are both sleeved with sealing rings 6221.

Referring to fig. 5, the push-up assembly 63 includes a second motor 631 and a second lead screw 632, the second motor 631 is mounted on the frame 4, specifically, the frame 4 further includes two vertical plates 47 vertically spaced and fixedly mounted on the lower surface of the bottom plate 41, and the second motor 631 is fixedly mounted on a side wall of one of the vertical plates 47. The second screw rod 632 is coaxially and fixedly arranged with an output shaft of the second motor 631, the second screw rod 632 drives the moving plate 46 to be close to or far away from the placing seat 5, the moving plate 46 is connected with the frame 4 through the second linear guide rails 48 in a sliding manner, the second linear guide rails 48 are arranged in two numbers, the second guide rails of the two second linear guide rails 48 are respectively vertically arranged on the side walls opposite to the two vertical plates 47, the second sliders of the two second linear guide rails 48 are respectively and fixedly arranged on the side walls on the two sides of the moving plate 46, and the second guide rails are vertically arranged with the second screw rod 632. The second motor 631 drives the moving plate 46 to move along the second guide rail through the second lead screw 632, so as to improve the stability of the sample outlet butt joint 461 on the moving plate 46 in butt joint with the microfluidic chip 3.

Referring to fig. 5, the push-up assembly 63 further includes a cross arm 633 vertically disposed in the frame 4, and the cross arm 633 is driven to rotate by a second motor 631 and a second lead screw 632 to push the moving plate 46 to move up and down. The second motor 631 drives the cross arm 633 installed in the frame 4 to rotate through the second screw 632, thereby pushing the moving plate 46 to move up and down, thereby reducing an installation space in a moving direction of the moving plate 46. Specifically, a connecting plate 49 is fixed between the two risers 47, and a cross arm 633 is provided between the connecting plate 49 and the moving plate 46. The cross arm 633 comprises a first arm 6331 and a second arm 6332, the first arm 6331 and the second arm 6332 are connected in a cross rotation manner, a first lug 6333 is fixed on the surface of the moving plate 46, one end of the first arm 6331 is hinged to the first lug 6333, a pushing block 6334 is connected to the second screw 632 in a threaded manner, and the other end of the first arm 6331 is hinged to the pushing block 6334. The connecting plate 49 is fixedly provided with a second lug 6335, the first lug 6333 and the second lug 6335 are oppositely arranged, one end of the second arm 6332 is hinged to the second lug 6335, the moving block 6336 is connected to the moving plate 46 of the pushing block 6334 in a sliding manner, and the other end of the second arm 6332 is hinged to the moving block 6336.

Referring to fig. 4 and 6, further, a waste liquid head 6211 is disposed on the docking plate 621, a plurality of three-way valves 6212 are disposed on the docking plate 621, a liquid inlet of each three-way valve 6212 is communicated with the collecting docking head 622, one liquid outlet of each three-way valve 6212 is communicated with the liquid outlet pipe, and the other liquid outlet of each three-way valve 6212 is communicated with the waste liquid head 6211. The collection butt 622 and the waste liquid head 6211 are gated by a three-way valve 6212 to perform blood collection or waste liquid collection and discharge.

Referring to fig. 7, a communication channel 6213 is disposed inside the docking plate 621, the communication channel 6213 communicates with the liquid outlets corresponding to the plurality of three-way valves 6212, and the liquid outlet end of the communication channel 6213 communicates with the waste liquid head 6211. The plurality of collecting butt joints 622 realize the collection of the blood samples or the waste liquid of the plurality of microfluidic chips 3, so that the collection efficiency of the blood samples and the waste liquid is improved.

Referring to fig. 8, a waste liquid receiving hopper 7 is provided below a waste liquid head 6211, which is fixedly mounted between two vertical plates 47. The waste liquid receiving bucket 7 is obliquely arranged towards one side, a waste liquid draining joint 71 is arranged on the side wall of the waste liquid receiving bucket 7 positioned on the oblique side, and the waste liquid draining joint 71 drains waste liquid through a pipeline.

The implementation principle of the collecting mechanism for the chip capture cells in the embodiment of the application is as follows: the microfluidic chip 3 is placed on the placing seat 5, the placing seat 5 is driven by the driving mechanism to be located in the frame 4, the moving plate 46 is driven by the push-up assembly 63 to move upwards, the sample outlet butt joint 461 and the collection butt joint 622 are in butt joint communication with the microfluidic chip 3, a blood sample is injected into the microfluidic chip 3 through the injection pump mechanism to complete cell capture, and then the captured blood sample is guided into the collection pipe 613 to be collected through the butt joint assembly 62 in butt joint communication with the sample outlet hole of the microfluidic chip 3, so that the efficiency of blood sample collection in the microfluidic chip 3 is improved.

The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims (8)

1. A collection mechanism for capturing cells on a chip, comprising: including being used for setting up collecting seat (61) placing seat (5) and being used for setting up butt joint subassembly (62) at moving plate (46), place seat (5) and be used for placing micro-fluidic chip (3), collecting seat (61) are provided with collecting pipe (613) that can get and put, butt joint subassembly (62) are used for with the play appearance hole butt joint intercommunication of micro-fluidic chip (3), the mouth of pipe of collecting pipe (613) corresponds the setting with the mouth of pipe of the play appearance pipe (623) of butt joint subassembly (62).

2. The chip-captured cell collection mechanism of claim 1, wherein: the butt joint assembly (62) is located below the placing seat (5), a collecting butt joint head (622) of the butt joint assembly (62) is used for being in butt joint communication with a sample outlet hole of the micro-fluidic chip (3), and a sample outlet pipe (623) of the butt joint assembly (62) is located above the collecting pipe (613).

3. The chip-trapping cell collection mechanism of claim 2, wherein: butt joint subassembly (62) still include butt joint board (621), collect butt joint (622) and go out appearance pipe (623) and all be connected with butt joint board (621), and collect butt joint (622) and go out appearance pipe (623) and communicate each other through butt joint board (621), butt joint board (621) fixed connection is on movable plate (46), just collect butt joint (622) and pass and extend to movable plate (46) top, movable plate (46) move up through push-up subassembly (63) to make the butt joint of appearance hole butt joint intercommunication of collection butt joint (622) and micro-fluidic chip (3).

4. The chip-captured cell collection mechanism of claim 3, wherein: the joint plate (621) is provided with a waste liquid joint (6211), the joint plate (621) is provided with a three-way valve (6212), a liquid inlet of the three-way valve (6212) is communicated with the collection joint (622), one liquid outlet of the three-way valve (6212) is communicated with a liquid outlet pipe, and the other liquid outlet of the three-way valve (6212) is communicated with the waste liquid joint (6211).

5. The chip-capturing cell collection mechanism of claim 4, wherein: place seat (5) and be used for interval placement a plurality of micro-fluidic chip (3), it sets up a plurality ofly to collect butt joint (622) and three-way valve (6212), and is a plurality of collect butt joint (622) and three-way valve (6212) interval fixed the setting on buttjunction board (621), and every collects butt joint (622) and communicates with the appearance hole of play of the micro-fluidic chip (3) that corresponds respectively, buttjunction board (621) inside is provided with communicating channel (6213), communicating channel (6213) communicates with the liquid outlet that a plurality of three-way valve (6212) correspond, the play liquid end and waste liquid head (6211) the intercommunication of communicating channel (6213).

6. The chip-captured cell collection mechanism of claim 4, wherein: a waste liquid receiving hopper (7) is arranged below the waste liquid head (6211).

7. The chip-captured cell collection mechanism of claim 6, wherein: the waste liquid receiving hopper (7) is obliquely arranged towards one side, and a waste liquid drainage joint (71) is arranged on the side wall of the waste liquid receiving hopper (7) positioned on the oblique side.

8. The chip-captured cell collection mechanism of claim 2, wherein: the collecting seat (61) is provided with a placing groove (612), and the collecting pipe (613) is placed in the placing groove (612).

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