CN2166135Y - Hollow fibre tube circulating cell culture system - Google Patents
Hollow fibre tube circulating cell culture system Download PDFInfo
- Publication number
- CN2166135Y CN2166135Y CN 92219587 CN92219587U CN2166135Y CN 2166135 Y CN2166135 Y CN 2166135Y CN 92219587 CN92219587 CN 92219587 CN 92219587 U CN92219587 U CN 92219587U CN 2166135 Y CN2166135 Y CN 2166135Y
- Authority
- CN
- China
- Prior art keywords
- pipeline
- tubular fibre
- cell culture
- peristaltic pump
- nutrient solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a hollow fibre pipeline circulating cell culture system, which belongs to the biological engineering field. The enforcement of the utility model thoroughly changes the structure of traditional cell culture bottles; cells flow in a hollow pipeline made of hollow fibre materials, while culture fluid flows conversely out of the pipeline. The power is finished by a double-pipeline peristaltic pump. The long-time mass culture of the cells is realized; the activity is higher; the pollution chance in culture process is reduced; culture mediums can be exchanged no need of the conditions of centrifugation, etc., (if the utility model is used for producing monoclonal antibodies, the monoclonal antibodies can be simultaneously extracted), which saves man power and material resources.
Description
The utility model discloses a kind of histocyte culture systems, it belongs to technical field of bioengineering.
Cultured tissue cell in the present laboratory is used a kind of horizontal bottle (being made by the nontoxic transparent organic materials of glass type) of flat transparent usually, when the cultured tissue cell inoculating histocyte in the flat bottle that contains nutrient solution.Through under the suitable culture condition (temperature, humidity, pH value etc.), histocyte breeding growth, it is all right that such culture condition is only done general qualitative examination.But make the culturing bottle of current biotherapy active cells with it, what just show is weak and incompetent.Even what the gram Schwann Cells culturing bottle of 800ml also only had a 250ml effectively utilizes volume, if biotherapy needs 5 * 10 a course of treatment
9Individual cell just needs 5000ml to nutrient solution, will use more than 20 bottle so--the full 40,000 yuan of CO2 incubators just having bought of an expense that occupy.And cell grows into certain density will divide bottle.Divide bottle once just to increase the primary pollution chance.If it is just difficult especially (when not outwelling the certain density stimulant of raffinate restock costliness that you want the raffinate after the cell growth utilization is outwelled, just can count this partial volume, thereby cause significant wastage, make also that to cultivate cost high) generally to pass through such process: " celliferous nutrient solution is poured into centrifugal bottle → centrifugal → abandon lean solution → cell is put into the new liquid continuation of new culturing bottle-addings cultivation ".Loaded down with trivial details operation, huge time waste, " headache " made us in increasing of opportunities for contamination really.Traditional besides Tissue Culture Flask, inner nutrient solution do not flow, and cause cell to utilize nutritive medium and gaseous interchange insufficient at the bottom of cell sinks to bottle, cause that reproduction speed is slow, activity is not high, and the output under same cost drops into that makes is too low.
The purpose of this utility model is exactly to overcome the shortcoming that above-mentioned tradition is organized Tissue Culture Flask, and the structure of former flat bottle is thoroughly improved, and designs a kind of removable parts nutrient solution and does not need centrifugal can finishing, and is simple to operate; It is easier that cell absorbs the nutritive ingredient exchanging gas in the growth of bottle internal recycle.The cultured cells rate of propagation is faster, and activity is higher.And the saving human and material resources, the histocyte culturing bottle of minimizing opportunities for contamination.
Task of the present utility model is that the nontoxic transparent organic materials of usefulness that following scheme realizes is done a garden cylindrical outer cover that length is suitable, along its axis, part is full of thick 0.1-0.5 millimeter, long with shell with the long hollow pipe made from tubular fibre (the filtration aperture of tubular fibre material is the 0.1-0.4 micron), sealed at both ends back (making the circulation cultured cells only can enter hollow tubular fibre pipeline), respectively keep an opening at the shell two ends, connect two mouthfuls with sebific duct, add a two pass peristaltic pump (another road is left nutrient solution for and used) on it, make the nutrient solution that contains cell flow to the other end, and cell can not flow out in hollow fiber conduit from an end of tubular fibre pipeline.There is the T-valve of controlling flow calorimeter and being convenient to cell adding discharge respectively to open a mouth at the bottom side and the other end upside of garden cylindrical outer cover again on the sebific duct, connects, sebific duct is placed on another road of above-mentioned two pass peristaltic pump with sebific duct.The control flow calorimeter is arranged on this root sebific duct and be convenient to the T-valve of substratum adding or eliminating and guarantee cell opposite with the flow direction of nutrient solution.Open two bigger mouths in order to exchanging gas on cylindrical outer cover top, garden.
The utility model product--tubular fibre pipeline circulating type cell culture systems is because the pipeline (the filtration aperture 0.1-0.4 micron of wall) that adopts tubular fibre to make, during cultivation the part nutrient solution is separated with cell, simultaneously an external two pass peristaltic pump flows cell in the tubular fibre pipeline and the outer nutrient solution of pipeline to make like this (1) thereby does not need condition such as centrifugal and provide for simple replacement of the part lean solution stimulant consumption of the costliness of promoting growth of cell breeding is reduced in culturing process.If (making monoclonal antibody with this device, so the monoclonal antibody that will collect just of lean solution) meaning the cultivation cost and reducing.(2) nutrient solution and cell flow and make cell cultures more near the human internal environment.(3) cell growth absorption nutritive ingredient exchanging gas is abundant.(4) the fast and maintenance activity highly of cell proliferation.(5) simple to operate, save human and material resources, reduce opportunities for contamination (6) cell and be in flow state, cultured cells density increases, and reduces taking CO relatively
2The volume of incubator.(7) cell cultures of especially suitable long period.The enforcement of the utility model patent will make the each side levels such as equipment of domestic tumor biotherapy that a bigger raising is arranged.
Accompanying drawing of the present utility model has been described the structure of this histocyte culture systems: mainly contain three parts among the figure: tubular fibre part, twin flue peristaltic pump, connecting tube.Tubular fibre partly has nontoxic transparent organic materials shell (1), the opening (2) (3) of handing-over gas; Wandering cells enters inlet (4) outlet (5) of tubular fibre part; Nutrient solution enters inlet (6) outlet (7) of tubular fibre part: hollow tubular fibre pipeline (8) in the shell (1) (these pipelines are made by the tubular fibre material, and material filters aperture between the 0.1-0.4 micron).Twin flue peristaltic pump (9), nontoxic transparent, the soft pipeline (10) that organic materials is manufactured of connection opening (4) (5), with and on control flow calorimeter (11), T-valve (12), the pipeline (12) of connection opening (6) (7), with and on control flow calorimeter (14), T-valve (15).
The utility model is implemented as follows: the long 30cm of organic materials shell, diameter 5cm.The diameter 0.2mm of hollow tubular fibre pipeline, long 28cm, the filtration aperture of tubular fibre material is the 0.1-0.4 micron.The flow velocity of cell be 0.5-1 rice/minute.Connecting tube is nontoxic, transparent, pressure hose.
Claims (3)
1, a kind of tubular fibre pipeline circulating type cell culture systems mainly comprises the garden cylindrical outer cover of being made by nontoxic transparent organic materials (1), and feature of the present utility model is made up of three parts: tubular fibre part, twin flue peristaltic pump, connecting tube; Tubular fibre partly has the opening (2) (3) of exchanging gas, and wandering cells enters inlet (4) outlet (5) of tubular fibre part; Nutrient solution enters inlet (6) outlet (7) of tubular fibre part, hollow tubular fibre pipeline (8) (these pipelines are to be made by the tubular fibre material, and the filtration aperture of material is the 0.1-0.4 micron); Twin flue peristaltic pump (9); Nontoxic, the soft pipeline (10) that transparent organic material is made of connection opening (4) (5), control flow calorimeter (11) is arranged on it, be convenient to the T-valve (12) that added or emit by cultured cells, nontoxic, the soft pipeline (13) that transparent organic material is made of connection opening (6) (7), with and on control flow calorimeter (14), be convenient to add or get rid of the T-valve (15) of substratum.
2,, it is characterized in that only allowing cell in pipeline, flow, and can not go to outside the pipeline in the mobile nutrient solution by the described tubular fibre pipeline of claim 1 circulating type cell culture systems; The filtration aperture of tubular fibre material is the 0.1-0.4 micron, make pipeline after, materials such as large protein molecule can the free two-way tube wall that passes through.
3, by the described tubular fibre pipeline of claim 1 circulating type cell culture systems, it is characterized in that also comprising that the twin flue peristaltic pump provides power, make the outer nutrient solution of tubular fibre pipeline interior (containing cell) in the two-way flow of tubular fibre part, and can drive the in parallel or series connection use of a plurality of tubular fibres parts with a twin flue peristaltic pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92219587 CN2166135Y (en) | 1992-08-13 | 1992-08-13 | Hollow fibre tube circulating cell culture system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92219587 CN2166135Y (en) | 1992-08-13 | 1992-08-13 | Hollow fibre tube circulating cell culture system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2166135Y true CN2166135Y (en) | 1994-05-25 |
Family
ID=33766803
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 92219587 Expired - Fee Related CN2166135Y (en) | 1992-08-13 | 1992-08-13 | Hollow fibre tube circulating cell culture system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2166135Y (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100404862C (en) * | 2003-09-29 | 2008-07-23 | 博士伦公司 | Peristaltic pump fitment for attachment to an aspirant collection bag |
| CN103031250A (en) * | 2012-10-17 | 2013-04-10 | 浙江赛尚医药科技有限公司 | Cell culture pipeline device and automatic cell culture system |
| CN105132374A (en) * | 2015-07-28 | 2015-12-09 | 浙江奥瑞健生物技术有限公司 | Neural stem cell medium change system and medium change method for neural stem cell culture |
| CN105385598A (en) * | 2015-11-30 | 2016-03-09 | 赵明光 | Human brain arteriovenous malformation biomechanics model and in vitro establishing method thereof |
| CN107904170A (en) * | 2017-12-18 | 2018-04-13 | 上海白泽医疗器械有限公司 | Media to cell culture modules and cell culture system |
| CN109266548A (en) * | 2018-10-11 | 2019-01-25 | 广州洁特生物过滤股份有限公司 | A kind of dynamic cell culture device and cell culture system |
| CN109679834A (en) * | 2019-01-07 | 2019-04-26 | 赵涌 | Hollow fiber conduit and method for external large-scale production red blood cell |
| CN111592985A (en) * | 2020-04-28 | 2020-08-28 | 郑州伊美诺生物技术有限公司 | Continuous culture device and culture method for hybridoma cells |
-
1992
- 1992-08-13 CN CN 92219587 patent/CN2166135Y/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100404862C (en) * | 2003-09-29 | 2008-07-23 | 博士伦公司 | Peristaltic pump fitment for attachment to an aspirant collection bag |
| CN103031250A (en) * | 2012-10-17 | 2013-04-10 | 浙江赛尚医药科技有限公司 | Cell culture pipeline device and automatic cell culture system |
| CN103031250B (en) * | 2012-10-17 | 2015-06-17 | 浙江赛尚医药科技有限公司 | Cell culture pipeline device and automatic cell culture system |
| CN105132374A (en) * | 2015-07-28 | 2015-12-09 | 浙江奥瑞健生物技术有限公司 | Neural stem cell medium change system and medium change method for neural stem cell culture |
| CN105385598A (en) * | 2015-11-30 | 2016-03-09 | 赵明光 | Human brain arteriovenous malformation biomechanics model and in vitro establishing method thereof |
| CN107904170A (en) * | 2017-12-18 | 2018-04-13 | 上海白泽医疗器械有限公司 | Media to cell culture modules and cell culture system |
| CN109266548A (en) * | 2018-10-11 | 2019-01-25 | 广州洁特生物过滤股份有限公司 | A kind of dynamic cell culture device and cell culture system |
| CN109266548B (en) * | 2018-10-11 | 2022-04-05 | 广州洁特生物过滤股份有限公司 | Dynamic cell culture device and cell culture system |
| CN109679834A (en) * | 2019-01-07 | 2019-04-26 | 赵涌 | Hollow fiber conduit and method for external large-scale production red blood cell |
| CN111592985A (en) * | 2020-04-28 | 2020-08-28 | 郑州伊美诺生物技术有限公司 | Continuous culture device and culture method for hybridoma cells |
| CN111592985B (en) * | 2020-04-28 | 2024-02-06 | 郑州伊美诺生物技术有限公司 | Continuous culture device and method for hybridoma cells |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5958761A (en) | Bioreactor and system for improved productivity of photosynthetic algae | |
| CN102134553B (en) | Tubular photobioreactor and system and method for culturing microalgae cells | |
| CN2166135Y (en) | Hollow fibre tube circulating cell culture system | |
| CN104560695A (en) | Tubular photo-bioreactor based on built-in fluid driven rotor | |
| CN112919641B (en) | Method and treatment device for denitrification and dephosphorization by utilizing microalgae | |
| CN201778022U (en) | Oil-rich microalgae culture device | |
| CN111763604B (en) | Pig farm fermentation tail liquid continuous treatment system for efficiently culturing coupled microalgae and operation process thereof | |
| CN104560637A (en) | Rotatable film-forming type microalgae photosynthetic reactor | |
| CN2234443Y (en) | Spirulina photosynthesis reactor | |
| CN103602579B (en) | Pipeline-type photobioreactor for scale culture of microalgae | |
| Chatterjee et al. | Simplified acoustic window mist bioreactor | |
| CN106497783A (en) | A kind of dynamic circulation cell culture apparatuses and cell culture processes | |
| CN103525686A (en) | Composite reinforced microalgae photobioreactor based on hollow light pipes | |
| CN101096639B (en) | Multi-group fiber pipes even distributed multifunctional hollow fiber film pipe bioreactor | |
| CN111676126B (en) | Airlift photobioreactor for microalgae culture | |
| CN104450492B (en) | The bioreactor of a kind of gradient illumination and application thereof | |
| CN2319413Y (en) | Pipeline gas raising magnetic treatment optical biological reactor microalgae breeding appts. | |
| CN102911856B (en) | Tangential casing built-in aeration photo-bioreactor applicable to micro algae high-efficiency culture | |
| CN204417511U (en) | The tubular type bioreactor of built-in rotor is driven based on fluid | |
| AU681243B2 (en) | A bioreactor and system for improved productivity of photosynthetic algae | |
| CN2394915Y (en) | Bubble tower type light biological reactor | |
| CN203238266U (en) | Photobioreactor device for culturing microalgae in high density | |
| CN105087374A (en) | Carbon source supply system for spirulina cultivated in pool | |
| CN106635768B (en) | Biological microalgae photosynthetic reactor and its application method | |
| CN204529840U (en) | A kind of rotatable biofilm declines algae photosynthetic reactor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |