CN216594884U - Indoor simulated earth pillar experimental device under freeze-thaw cycle condition - Google Patents
Indoor simulated earth pillar experimental device under freeze-thaw cycle condition Download PDFInfo
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- CN216594884U CN216594884U CN202123391690.2U CN202123391690U CN216594884U CN 216594884 U CN216594884 U CN 216594884U CN 202123391690 U CN202123391690 U CN 202123391690U CN 216594884 U CN216594884 U CN 216594884U
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Abstract
The utility model provides an indoor simulation earth pillar experimental apparatus under freeze thawing cycle condition, the earth pillar experimental apparatus with the earth pillar heat preservation layer of cylinder tube-shape is arranged in the high-low temperature alternation test box, the inner cavity mark of the earth pillar experimental apparatus is five experimental soil sample layers with even interval from bottom to top, each experimental soil sample layer is provided with a sampling tube communicated with the outside of the earth pillar experimental apparatus, the sensor probes of five earth pillar temperature parameter monitors sequentially extend into the experimental soil samples in the corresponding experimental soil sample layers through the top openings of the earth pillar experimental apparatus; on the basis of guaranteeing the authenticity and effectiveness of the freeze-thaw cycle conditions, the soil detection technology is combined with an indoor soil column simulation experiment, and the properties of soil with different depths in the soil column and the change condition of survival of microorganisms under the freeze-thaw cycle are accurately acquired.
Description
Technical Field
The utility model belongs to the technical field of soil freeze-thaw cycle simulation, a indoor simulation earth pillar experimental apparatus under freeze-thaw cycle condition is related to.
Background
Freeze-thawing is ubiquitous in the world, in high latitudes and at high altitudes. The freeze-thaw action refers to the process of thawing and freezing continuously along with seasonal changes or day and night alternation in the soil surface layer and a soil layer with a certain depth in a low-temperature environment, and can greatly change the physicochemical properties of the soil, influence the activity of microorganisms and further influence the geochemical cycle process of soil carbon and nitrogen organisms. The freeze-thaw cycle is a common natural phenomenon in seasonal frozen soil zones in northern China, and usually occurs in early spring or late autumn. However, due to the limitation of the current technology, most of domestic research only analyzes the problem of microbial contamination in water under the condition of freeze-thaw cycle, and few researchers do changes about soil properties and survival of soil microbes under the condition of simulated freeze-thaw cycle. This is due in part to the fact that there is currently no complete earth pillar experimental set that can simulate freeze-thaw cycling conditions indoors.
The freeze-thaw cycle condition in soil in northeast China is more consistent with the actual existence state of pathogenic bacteria in soil. Therefore, understanding the change and the influence mechanism of the survival behavior of the microorganisms in the vertical direction of different soil depths under the freeze-thaw cycle condition is helpful for comprehensively understanding the persistence of soil microorganism pollution under the freeze-thaw cycle condition, and has important significance for deeply understanding the soil health and the soil microecological safety. At present, the soil column experimental device is small in size due to the fact that the general soil column ruler is small in size, the diameter of most of the soil columns is 10 cm, the height of the soil columns is below 50 cm, the bottom of the soil columns is provided with an opening for collecting a soil sample, the simulated soil volume of the soil columns is not large enough, and reasonable soil sampling ports are not designed. After freeze thawing cycle, soil at different depths is easy to mix when sampling from the bottom, so that cross contamination is caused, and meanwhile, after sampling from the bottom of the soil column, the volume of the soil in the soil column is obviously reduced. In order to guarantee the reliability of the later-stage experimental result, soil backfilling is needed, but the backfilling of the later-stage soil is very difficult due to the fact that the bottom sampling is conducted, and therefore the survival situation of pathogenic bacteria in the soil with different depths is difficult to accurately analyze so as to know the pollution risk of the pathogenic bacteria in the soil with different depths. In addition, the existing soil column experimental device is not provided with a soil column heat-insulating layer, so that the accuracy of later-stage experimental results is influenced.
Disclosure of Invention
The utility model discloses an overcome the above-mentioned problem that prior art exists, provide an indoor simulation earth pillar experimental apparatus under freeze thawing cycle condition. The experimental device combines the soil detection technology with the indoor soil column simulation experiment on the basis of guaranteeing the authenticity and the effectiveness of the freeze-thaw cycle condition, and accurately obtains the properties of soil with different depths in the soil column and the change condition of the survival of microorganisms under the freeze-thaw cycle.
The utility model discloses an adopt following technical scheme to realize:
the indoor simulated soil column experimental device under the freeze-thaw cycle condition comprises a high-low temperature alternating test box, wherein a hollow cylindrical soil column experimental device with an open top and a closed bottom is arranged in the high-low temperature alternating test box, the soil column experimental device is provided with a soil column heat-insulating layer formed by filling heat-insulating materials, four annular scale marks are uniformly marked on the inner wall of the soil column experimental device from bottom to top at intervals to mark an inner cavity of the soil column experimental device as five experimental soil sample layers with uniform intervals, so that experimental soil samples are sequentially added into each experimental soil sample layer from bottom to top in a layering manner, five sampling pipes uniformly arranged from bottom to top at intervals penetrate through the soil column heat-insulating layer from the outside of the soil column experimental device and extend into the corresponding experimental soil sample layer of the soil column experimental device, and five soil column temperature parameter monitors are further arranged, and terminal equipment for displaying data of the five soil column temperature parameter monitors are arranged inside the high-low temperature alternating test box, Outside the earth pillar experimental apparatus, the sensor detecting heads of the five earth pillar temperature parameter monitors for monitoring the temperature and the water content of the experimental soil sample sequentially extend into the experimental soil sample in the corresponding experimental soil sample layer through the top opening of the earth pillar experimental apparatus.
The further technical scheme comprises the following steps:
four angles of high low temperature alternation proof box bottom all are provided with the base pulley.
The soil column experimental device is formed by sleeving two hollow PVC pipes with different diameters, different heights and closed bottoms, and an annular gap formed between the pipe walls of the two PVC pipes and a gap formed between the bottoms of the two PVC pipes are filled with heat-insulating materials to form a soil column heat-insulating layer.
The heat insulation material in the soil column heat insulation layer is a polyurethane heat insulation material.
And a sealing ring is arranged between the sampling tube and the soil column heat-insulating layer to ensure the air tightness.
The sampling tube is arranged at the opening part outside the heat-insulating layer of the earth pillar, and a rubber piston which can be inserted and pulled out is arranged at the opening part and is used for opening and closing the opening of the sampling tube.
The temperature control range of the high-low temperature alternating test box is-40 ℃ to 120 ℃.
The height of each sampling tube from the bottom of the experimental soil sample layer is half of the height of the experimental soil sample layer.
Compared with the prior art, the beneficial effects of the utility model are that:
1) the utility model provides an indoor simulation earth pillar experimental apparatus under freeze-thaw cycle condition, this experimental apparatus can fill the blank, set up the simulation earth pillar experimental apparatus under one set of freeze-thaw cycle condition in indoor, can realize the influence that simulation freeze-thaw cycle changes in the vertical direction to soil physicochemical property and microorganism survival in indoor, provide probably to the law that the pathopoiesia microbe microorganism survives in the typical soil of research northeast region seasonally frozen soil zone;
2) the utility model provides an indoor simulation earth pillar experimental apparatus under freeze thawing cycle condition combines high low temperature alternation proof box and indoor simulation earth pillar device, increases the temperature control device of indoor earth pillar experimental system, simulates the influence of freeze thawing cycle in northeast region on soil physicochemical property and microorganism survival;
3) the utility model provides an indoor simulation earth pillar experimental apparatus under freeze thawing cycle condition, overall structure is comparatively simple, the cost is showing and is reducing, and can simulate the influence effect of different freeze thawing cycle periods to each index of soil through setting up different freeze thawing time and temperature, the practicality is stronger;
4) the utility model provides an indoor simulation earth pillar experimental apparatus under freeze thawing cycle condition, through the setting of earth pillar heat preservation, can reduce the influence of other factors, promote the accuracy of experiment;
5) the utility model provides an indoor simulation earth pillar experimental apparatus under freeze-thaw cycle condition, experimental soil sample is packed in layers, can conveniently simulate the change situation of physical and chemical properties and microorganism survival in the vertical direction of soil under freeze-thaw cycle condition;
6) the utility model provides a pair of indoor simulation earth pillar experimental apparatus under freeze thawing cycle condition, through the test of sampling many times of the experimental soil sample of sampling tube to co-altitude, can know the change condition that the physicochemical property and the microorganism of different degree of depth soil survive in the freeze thawing cycle in-process in a certain time, can form dynamic change process through the test of taking a sample in succession, have higher reference value.
Drawings
The invention will be further described with reference to the accompanying drawings:
fig. 1 is a schematic view of the overall structure of an indoor simulated soil column experimental device under the freeze-thaw cycle condition provided by the utility model;
FIG. 2 is a schematic structural view of the soil column experimental device filled with experimental soil samples;
fig. 3 is the utility model provides a soil column temperature parameter monitor schematic structure.
In the figure: 1. the device comprises a high-low temperature alternating test box, 2 a soil column experimental device, 3 a soil column heat-insulating layer, 4 a soil column temperature parameter monitor, 5 a sampling tube, 6 an experimental soil sample and 7 a base pulley.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings:
the utility model provides a pair of indoor simulation earth pillar experimental apparatus under freeze thawing cycle condition, as shown in fig. 1, fig. 2 and fig. 3, include: high low temperature alternation proof box 1, earth pillar experimental apparatus 2, earth pillar heat preservation 3, earth pillar temperature parameter monitor 4, sampling tube 5 and base pulley 7.
High low temperature alternation proof box 1 is temperature regulating equipment, and high low temperature alternation proof box 1's length, width and height are 60cm, 70cm respectively, for whole experimental apparatus's major structure, for other structures provide support and connection, and four angles of high low temperature alternation proof box 1 bottom are equipped with four base pulleys 7 in order to make things convenient for the experimental apparatus to remove. The high-low temperature alternating test box 1 controls the internal temperature thereof to simulate the soil property and the survival change condition of microorganisms under the freeze-thaw cycle.
The high-low temperature alternating test chamber 1 can adopt a temperature control device which is commonly available in the market. On the basis, considering the requirement of the high-low temperature alternating test box 1 as a support main body, as an optional implementation mode, the main body of the high-low temperature alternating test box 1 is made of stainless steel, the heat insulation material is made of superfine glass wool, and the temperature control range of the high-low temperature alternating test box 1 is-40-120 ℃. The thickness of the bottom of the high-low temperature alternating test box 1 is 100mm, and the thickness of the side wall is 50 mm.
And the earth pillar experimental device 2 is arranged inside the high-low temperature alternating test box 1. The earth pillar experimental apparatus 2 is two different diameters, the open-top of co-altitude, thereby the open-top that the closed hollow PVC pipe in bottom inlayed the cover and together formed, the closed hollow cylinder in bottom, in this embodiment, the diameter of two PVC pipes is 110mm and 160mm respectively, wherein the height of inlayer PVC pipe is 60cm, the height of outer PVC pipe is 62.5cm, the clearance between the pipe wall of two PVC pipes is 25mm, the clearance between two PVC socle portions is 25mm, earth pillar experimental apparatus 2 mainly uses as the container that holds experimental soil appearance 6. Therefore, the soil column experimental device 2 is a hollow cylinder with an inner diameter of 110mm, an outer diameter of 160mm, a height of 62.5cm, an inner cavity height of 60cm and a wall thickness of 25mm, and the inner cavity of the soil column experimental device 2 is used for arranging the experimental soil sample 6. 2 size and specification of earth pillar experimental apparatus are great, and simulation soil is bulky enough, more can reflect the true situation of soil, and the later stage sample also has more the representativeness.
As shown in fig. 2, the experimental soil samples 6 are filled in layers, the internal cavity of the soil column experimental device 2 with the height of 60cm is marked as one experimental soil sample layer at intervals of 12cm, and five experimental soil sample layers are arranged in total so as to facilitate analysis of physicochemical properties of soil in the vertical direction and change conditions of microbial survival under the freeze-thaw cycle condition. In this embodiment, the mode that the adoption was followed supreme every interval 12cm mark four annular scale marks of annular scale marks altogether from supreme down on earth pillar experimental apparatus 2's inner wall and is marked these five experimental soil sample layers, earth pillar experimental apparatus 2 side is followed the height of the inside cavity of earth pillar experimental apparatus 2 and is equipped with five sampling tubes 5 from supreme interval down uniformly, sampling tube 5 passes earth pillar heat preservation 3 from earth pillar experimental apparatus 2 outside and stretches into in the experimental soil sample layer that earth pillar experimental apparatus 2 corresponds, every sampling tube 5 is the half of this experimental soil sample layer height apart from the height of the bottom on this experimental soil sample layer, in this embodiment, every sampling tube 5 is 6cm apart from the height of the bottom on this experimental soil sample layer, be provided with the sealing washer between sampling tube 5 and the earth pillar heat preservation 3 in order to guarantee the gas tightness, interval between two adjacent sampling tubes 5 is 12 cm.
The soil column heat-insulating layer 3 is formed by filling polyurethane heat-insulating materials into gaps formed after two PVC pipes with different diameters and different heights are embedded, the soil column heat-insulating layer is manufactured by adopting a polyurethane black and white material foaming agent in the embodiment, polyurethane black and white materials are prepared, mixed and poured into the black and white materials according to the proportion of the black and white materials (1:1 or more white materials), stirred for 2-5 seconds until liquid just shows white color, and quickly poured into the gaps formed after the two PVC pipes are embedded. After the black and white material mixed liquid starts to foam automatically for 3-5 minutes, the foaming is finished by taking the foaming temperature to be reduced to the normal temperature as the standard, the step can be operated for many times to obtain an even soil column heat-insulating layer 3, the annular gap formed between the pipe walls of two PVC pipes and the gap formed between the bottoms of the two PVC pipes are both 25mm and are filled with polyurethane heat-insulating materials, namely the thickness of the soil column heat-insulating layer 3 is 25mm, the integral initial condition of the soil column is ensured to be consistent, and the influence of other factors except the freeze-thaw cycle on the experimental result is eliminated.
Five earth pillar temperature parameter monitors 4 are used to monitor the temperature and water content of the experimental soil sample 6 in each experimental soil sample layer. The soil column temperature parameter monitor 4 comprises a sensor probe and a terminal device for displaying data. The temperature measuring range of the soil column temperature parameter monitor 4 is-50 ℃ to 70 ℃, the resolution is 0.1 ℃, and the accuracy is +/-1 ℃. Sensor detecting head and terminal equipment adopt wired connection, and the line length is 1.5m, for the convenience of setting up earth pillar temperature parameter monitor 4, we set up the sensor detecting head of five earth pillar temperature parameter monitors 4 and bury in proper order inside the experimental soil sample 6 on the experimental soil sample layer of the different degree of depth through the top of earth pillar experimental apparatus 2, and the terminal equipment of the display data of five earth pillar temperature parameter monitors 4 is located the outside of earth pillar experimental apparatus 2, the inside of high low temperature alternation proof box 1. So as to monitor the temperature and humidity change conditions of the experimental soil samples 6 on different layers in the soil column in the freeze-thaw cycle process.
Every sampling tube 5 is located the outside opening part of earth pillar heat preservation 3, is provided with a rubber piston that can insert and extract for open and seal the opening of sampling tube 5, be convenient for extract the soil sample test at any time, and can carry out the sample test under the condition that does not destroy freeze-thaw cycle experimentation, have fine practicality. Wherein, the sampling tube 5 is a steel tube with an inner diameter of 30mm and a length of 40 mm.
The indoor soil column simulation experimental device under the freeze-thaw cycle condition is used for researching the physicochemical properties of experimental soil samples at different depths in the soil column and the survival change condition of escherichia coli in the freezing and thawing processes under the freeze-thaw cycle condition, and the operation is as follows:
(1) obtaining a plurality of representative natural soil samples in northeast regions through field investigation and sampling, and measuring the field Water Holding Capacity (WHC) of the soil samples after the soil samples are naturally air-dried;
(2) weighing a certain amount of soil, adding escherichia coli bacterial liquid into the soil according to the mass ratio of 1%, enabling the water content of the added soil to reach 50% -60% of WHC, ensuring that the bacterial liquid and the soil are uniformly mixed, preventing the soil from being too much in water to influence filling of soil columns, and obtaining an experimental soil sample 6 through the setting;
(3) and (3) filling the experimental soil sample 6 with uniform bacteria liquid into the soil column by times (filling a soil sample with the height of 12cm each time), after each layer of soil sample is filled, extending a sensor probe of a soil column temperature parameter monitor 4 into the experimental soil sample 6 through the top opening of the soil column experimental device 2 and burying the sensor probe into the experimental soil sample 6, and compacting each layer of soil.
(4) The soil column experimental device 2 and the soil column temperature parameter monitor 4 which are provided with the experimental soil sample 6 are placed in a high-low temperature alternating test box 1, a certain freeze-thaw cycle period (such as 12 hours of freezing at-15 ℃ and 12 hours of melting at +10 ℃) is set by adjusting the high-low temperature alternating test box 1, and the freeze-thaw cycle temperature value is observed regularly. Taking out the experimental soil samples 6 of each layer from bottom to top through the sampling tubes 5 every 24 hours, respectively measuring the physicochemical properties and the survival quantity of escherichia coli of the experimental soil samples 6 of each layer, and continuously sampling in the experimental process until the quantity of the escherichia coli in the experimental soil samples 6 is lower than the soil detection limit (100 CFU/gdw);
(5) and repeating the steps, adjusting the temperature of the freeze-thaw cycle, and comparing.
Claims (8)
1. An indoor simulated earth column experimental device under a freeze-thaw cycle condition comprises a high-low temperature alternation test box (1) and is characterized in that a hollow cylindrical earth column experimental device (2) with an open top and a closed bottom is arranged in the high-low temperature alternation test box (1), the earth column experimental device (2) is provided with an earth column heat-insulating layer (3) formed by filling heat-insulating materials, four annular scale marks are uniformly marked on the inner wall of the earth column experimental device (2) from bottom to top at intervals to mark an inner cavity of the earth column experimental device (2) as five experimental soil sample layers with uniform intervals so as to sequentially add experimental soil samples (6) to each experimental soil sample layer from bottom to top in a layering manner, and five sampling pipes (5) uniformly arranged from bottom to top at intervals penetrate through the earth column heat-insulating layer (3) from the outside of the earth column experimental device (2) and extend into the experimental soil sample layer corresponding to the earth column experimental soil sample layer (2), still include five earth pillar temperature parameter monitors (4), five earth pillar temperature parameter monitors (4) are used for the terminal equipment of display data all to set up in the inside of high low temperature alternation proof box (1), the outside of earth pillar experimental apparatus (2), the sensor detecting head that five earth pillar temperature parameter monitors (4) are used for monitoring the temperature and the moisture content of experiment soil sample (6) stretches into in proper order in experiment soil sample (6) in the experiment soil sample layer that corresponds through the open-top of earth pillar experimental apparatus (2).
2. The indoor soil column experimental device for freeze-thaw cycle conditions, which is characterized in that base pulleys (7) are arranged at four corners of the bottom of the high-low temperature alternating test box (1).
3. An indoor soil column simulation experiment device under freeze-thaw cycle conditions, according to the claim 1, characterized in that the soil column experiment device (2) is composed of two hollow PVC pipes with different diameters, different heights, top openings and bottom closures, and the annular space formed between the pipe walls of the two PVC pipes and the space formed between the bottoms of the two PVC pipes are filled with heat insulation materials to form the soil column heat insulation layer (3).
4. The indoor soil column experimental device for simulation under freeze-thaw cycle condition according to claim 3, wherein the insulation material in the soil column insulation layer (3) is polyurethane insulation material.
5. An indoor simulated soil column experimental facility under freeze-thaw cycle conditions according to any one of claims 1 to 4, characterized in that a sealing ring is arranged between the sampling tube (5) and the soil column insulation layer (3) to ensure air tightness.
6. An indoor simulated soil column experimental facility under freeze-thaw cycle conditions according to any one of claims 1 to 4, characterized in that the opening of the sampling tube (5) outside the soil column insulation layer (3) is provided with a rubber piston capable of being inserted and pulled out for opening and closing the opening of the sampling tube (5).
7. The indoor simulated soil column experimental device under freeze-thaw cycle conditions as claimed in any one of claims 1 to 4, wherein the temperature control range of the high-low temperature alternating test chamber (1) is-40 ℃ to 120 ℃.
8. A soil column laboratory apparatus for laboratory simulation under freeze-thaw cycle according to any one of claims 1 to 4, wherein each sampling tube (5) has a height from the bottom of the experimental soil sample layer of one half of the height of the experimental soil sample layer.
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| CN202123391690.2U CN216594884U (en) | 2021-12-30 | 2021-12-30 | Indoor simulated earth pillar experimental device under freeze-thaw cycle condition |
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| CN202123391690.2U CN216594884U (en) | 2021-12-30 | 2021-12-30 | Indoor simulated earth pillar experimental device under freeze-thaw cycle condition |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115097101A (en) * | 2022-06-14 | 2022-09-23 | 西藏低碳草牧科技有限公司 | Experimental device is cultivateed to frozen soil original state earth pillar for a year |
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2021
- 2021-12-30 CN CN202123391690.2U patent/CN216594884U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115097101A (en) * | 2022-06-14 | 2022-09-23 | 西藏低碳草牧科技有限公司 | Experimental device is cultivateed to frozen soil original state earth pillar for a year |
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