CN216594439U - Transfer type high-flux ultrathin section staining instrument - Google Patents
Transfer type high-flux ultrathin section staining instrument Download PDFInfo
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- CN216594439U CN216594439U CN202122868527.4U CN202122868527U CN216594439U CN 216594439 U CN216594439 U CN 216594439U CN 202122868527 U CN202122868527 U CN 202122868527U CN 216594439 U CN216594439 U CN 216594439U
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Abstract
The utility model belongs to the technical field of transmission electron microscopes. The utility model provides a transfer type high-flux ultrathin section staining instrument, which comprises: a support platform; the supporting column is arranged at the center of the supporting platform and is fixed on the supporting platform; the center of the bottle cap platform is fixed at the other end of the support column; a rinsing tank, a dyeing bottle A and a dyeing bottle B, all fixed on the support platform and around the support column. The device can automatically perform dyeing liquor dyeing, rinsing, air drying, dyeing liquor re-dyeing, rinsing and air drying on batch loading nets inserted in the loading net rack, improves the working efficiency and saves the dyeing liquor.
Description
Technical Field
The utility model belongs to the technical field of transmission electron microscopes.
Background
When the transmission electron microscope is used for observing the inner ultrastructure of a biological sample cell, an ultrathin slice sample preparation technology is needed, the sample is prepared into an ultrathin slice with the thickness of less than 100nm and is carried on a carrying net with the diameter of about 3mm, the inner diameter of about 2.6nm, the ring width of about 0.2mm and the thickness of 0.02mm, then tweezers are used for clamping the ring width position of the carrying net as far as possible (mechanical damage to the biological slice is avoided to the greatest extent), and microscopic imaging observation can be carried out after dyeing operation. The dyeing process needs dyeing of two dye solutions, namely front and back dyeing, and after dyeing of each dye solution is finished, rinsing and air drying are needed, and the process is totally operated by 6 steps.
How to solve the problem of complex manual dyeing is always the difficult point and pain point of the net-loading dyeing technology.
SUMMERY OF THE UTILITY MODEL
In view of this, the dyeing instrument provided by the utility model takes two symmetric clamping type batch net carrying frames of the utility model ZL 201610366342.4 and the utility model ZL 202020027034.0 as core functional components, and establishes a mechanical transfer working mode on the basis, so as to complete the automatic operation of dyeing solution dyeing, rinsing, air drying, dyeing solution re-dyeing, rinsing and air drying on the batch net carrying frames inserted and placed on the net carrying frames.
The utility model provides a transfer type high-flux ultrathin section staining instrument, which comprises: a support platform; the supporting column is arranged at the center of the supporting platform and is fixed on the supporting platform; the center of the bottle cap platform is fixed at the other end of the support column; a rinsing tank, a dyeing bottle A and a dyeing bottle B, all fixed on the support platform and around the support column.
In a specific embodiment of the utility model, the bottle cap platform is provided with three stations, wherein the first station is a hollow hole, and the second station and the third station are matched with the bottle caps of the dye solution bottles A and B in size and dimension.
In a particular embodiment of the utility model, the rinse tank comprises: a rinsing liquid chamber A, a rinsing liquid inlet pipe interface, a connecting wall, a rinsing liquid chamber B and a waste liquid outlet; one side of the rinsing liquid chamber A is provided with a rinsing liquid inlet pipe interface, and the other side of the rinsing liquid chamber A is connected with the rinsing liquid chamber B through a connecting wall; a waste liquid outlet is formed in the bottom of the rinsing liquid chamber B; the connecting wall is provided with a plurality of holes or slits with small apertures, and liquid introduced from the rinsing liquid chamber A enters the rinsing liquid chamber B through the connecting wall in a direction close to parallel to achieve the purpose that the rinsing liquid rinses the carrier net of the carrier net rack in a direction approximately perpendicular to the carrier net of the carrier net rack; the waste liquid outlet is the same as the waste liquid pool and realizes that the waste liquid flows into the waste liquid pool.
In a specific embodiment of the utility model, the washing machine further comprises a waste liquid pool, wherein the waste liquid pool is arranged below the supporting platform and used for receiving liquid flowing out of the rinsing pool waste liquid outlet.
In a specific embodiment of the present invention, the method further comprises: the manipulator and 3 stepping motors; the manipulator and 3 stepping motors are arranged above the supporting platform, and the 3 stepping motors control the manipulator to move in X, Y, Z three directions, so that the manipulator can grab and move the articles; the manipulator is used for grabbing and grabbing bottle caps of the liquid discharge bottle A and the dye solution bottle B and realizing the cap covering and cap pulling operations; the manipulator can also perform grabbing and grabbing actions on the top covers of the functional components of the grid-carrying racks on the installed cards; the stepping motor is arranged on the keel frame.
In a specific embodiment of the present invention, the seasoning system comprises four corner and top fans.
The device can automatically perform dyeing liquor dyeing, rinsing, air drying, dyeing liquor re-dyeing, rinsing and air drying on batch loading nets inserted in the loading net rack, improves the working efficiency and saves the dyeing liquor.
Drawings
FIG. 1 is a view of a part of the apparatus of the dyeing apparatus. Wherein, 1 is the supporting platform, 2 is the support column, 3 is the bottle lid platform, 4 is the rinsing pond, 5 is dyeing bottle A, 6 is dyeing bottle B, 7 is the waste liquid pond, 8 is functional unit S.
Figure 2 is a view of a closure platform assembly.
FIG. 3 is a diagram of the rinse cell components. Wherein 41 is a rinsing liquid chamber A, 42 is a rinsing liquid inlet pipe interface, 43 is a connecting wall, 44 is a rinsing liquid chamber B, and 45 is a waste liquid outlet.
FIG. 4 is an overall view of the staining apparatus. Wherein 9 is a manipulator; 10 is a stepping motor x, 11 is a stepping motor y, and 12 is a stepping motor z.
Detailed Description
Example 1
The ultra-thin section dyeing appearance of transfer formula high flux includes:
a support platform;
the support column is arranged at the center of the support platform and fixed on the support platform, and the height of the support column is higher than the maximum height of the carrier rack and ensures that the carrier rack is stably suspended and does not contact with other parts;
the center of the bottle cap platform is fixed at the other end of the support column; the bottle cap platform is provided with three stations, wherein the first station is a hollow hole site and can clamp a dyeing functional part S in a sleeving manner, the top end of the functional part S is a bottle cap structure with the size and the shape consistent with those of bottle caps A and B of the dye bottles, and the lower end of the functional part S can be provided with an upper loading net rack in a clamping manner; the second station and the third station are matched with the bottle caps of the dye bottles A and B in size and dimension.
A rinsing tank, a dyeing bottle A and a dyeing bottle B, all fixed on the support platform and around the support column.
The lower end of the rinsing pool is provided with a liquid outlet or a pipeline and is communicated with the waste liquid pool; the rinsing pool is used for rinsing the net carrying frame. The rinsing pool comprises a rinsing liquid chamber A, a rinsing liquid inlet pipe interface, a connecting wall, a rinsing liquid chamber B and a waste liquid outlet; one side of the rinsing liquid chamber A is provided with a rinsing liquid inlet pipe interface, and the other side of the rinsing liquid chamber A is connected with the rinsing liquid chamber B through a connecting wall; a waste liquid outlet is formed in the bottom of the rinsing liquid chamber B; the connecting wall is provided with a plurality of holes or slits with small apertures, and liquid introduced from the rinsing liquid chamber A enters the rinsing liquid chamber B through the connecting wall in a direction close to parallel to achieve the purpose that the rinsing liquid rinses the carrier net of the carrier net rack in a direction approximately perpendicular to the carrier net of the carrier net rack; the waste liquid outlet is the same as the waste liquid pool and realizes that the waste liquid flows into the waste liquid pool.
Dyeing bottle A and dyeing bottle B, dyeing bottle A and dyeing bottle B are used for holding dyeing liquid and carry the net realization dyeing to the net carrier.
And the waste liquid tank is arranged below the supporting platform and is used for receiving liquid flowing out of the rinsing tank waste liquid outlet.
The manipulator and the 3 stepping motors are arranged above the supporting platform, and the 3 stepping motors control the manipulator to move in X, Y, Z three directions, so that the manipulator can grab and move the articles; the manipulator is used for grabbing and grabbing bottle caps of the liquid discharge bottle A and the dye solution bottle B and realizing the cap covering and cap pulling operations; the manipulator can also perform grabbing and grabbing actions on the top covers of the functional components of the grid-carrying racks on the installed cards; the stepping motor is arranged on the keel frame.
And the air drying system comprises four corners and top end fans, and is arranged around the supporting platform and used for air drying the slices of the net carrying rack net.
And the pipeline and the flowmeter are matched with the rinsing pool and control the flow rate and the flow velocity of the liquid in the rinsing pool.
5) The gas phase fluid system comprises a four-corner fan system matched with the air drying net carrying rack function and an air outlet at the top end of the box body.
The device can automatically perform dyeing liquor dyeing, rinsing, air drying, dyeing liquor re-dyeing, rinsing and air drying on batch loading nets inserted in the loading net rack, improves the working efficiency and saves the dyeing liquor.
Claims (6)
1. Transfer type high flux ultrathin section staining appearance, characterized by, includes:
a support platform;
the supporting column is arranged at the center of the supporting platform and is fixed on the supporting platform;
the center of the bottle cap platform is fixed at the other end of the support column;
a rinsing tank, a dyeing bottle A and a dyeing bottle B, all fixed on the support platform and around the support column.
2. The transfer-type high throughput ultrathin section stainer of claim 1, wherein the bottle cap platform has three stations, wherein the first station is a hollow hole, and the second station and the third station are matched with the size and dimensions of the bottle caps of the dye bottles a and B.
3. The transfer-type high throughput ultrathin section stainer of claim 1, wherein the rinse tank comprises:
a rinsing liquid chamber A, a rinsing liquid inlet pipe interface, a connecting wall, a rinsing liquid chamber B and a waste liquid outlet;
one side of the rinsing liquid chamber A is provided with a rinsing liquid inlet pipe interface, and the other side of the rinsing liquid chamber A is connected with the rinsing liquid chamber B through a connecting wall;
a waste liquid outlet is formed in the bottom of the rinsing liquid chamber B;
the connecting wall is provided with a plurality of holes or slits with small apertures, and the liquid introduced from the rinsing liquid chamber A enters the rinsing liquid chamber B through the connecting wall in a direction nearly parallel to the rinsing liquid chamber A so as to rinse the rinsing liquid in a direction nearly perpendicular to the carrier net of the carrier net;
the waste liquid outlet is the same as the waste liquid pool and realizes that the waste liquid flows into the waste liquid pool.
4. A transfer-type high throughput ultrathin section stainer as claimed in any one of claims 1 to 3 further comprising a waste reservoir below the support platform for receiving liquid from the rinse reservoir waste outlet.
5. The transfer-type high throughput ultrathin section stainer of any one of claims 1 to 3, further comprising:
the manipulator and 3 stepping motors;
the manipulator and 3 stepping motors are arranged above the supporting platform, and the 3 stepping motors control the manipulator to move in X, Y, Z three directions, so that the manipulator can grab and move the articles;
the manipulator is used for grabbing and grabbing bottle caps of the liquid discharge bottle A and the dye solution bottle B and realizing the cap covering and cap pulling operations;
the manipulator can also perform grabbing and grabbing actions on the top covers of the functional components of the grid-carrying racks on the installed cards;
the stepping motor is arranged on the keel frame.
6. The transfer-type high throughput ultrathin section stainer of any one of claims 1 to 3, further comprising an air drying system comprising four corner and top fans.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122868527.4U CN216594439U (en) | 2021-11-22 | 2021-11-22 | Transfer type high-flux ultrathin section staining instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122868527.4U CN216594439U (en) | 2021-11-22 | 2021-11-22 | Transfer type high-flux ultrathin section staining instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216594439U true CN216594439U (en) | 2022-05-24 |
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ID=81646170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122868527.4U Active CN216594439U (en) | 2021-11-22 | 2021-11-22 | Transfer type high-flux ultrathin section staining instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216594439U (en) |
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2021
- 2021-11-22 CN CN202122868527.4U patent/CN216594439U/en active Active
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