CN216585020U

CN216585020U - Many sample sizes nucleic acid extraction element

Info

Publication number: CN216585020U
Application number: CN202220446228.3U
Authority: CN
Inventors: 董从洋
Original assignee: Fujian Baimeng Medical Technology Co ltd
Current assignee: Fujian Baimeng Medical Technology Co ltd
Priority date: 2022-03-03
Filing date: 2022-03-03
Publication date: 2022-05-24
Anticipated expiration: 2032-03-03

Abstract

The utility model discloses a multi-sample nucleic acid extraction device, wherein a 96-hole deep hole plate is movably connected in two placing grooves, the 96-hole deep hole plate comprises an outer frame, a plurality of identical square grooves are arranged on two sides of an inner cavity of the outer frame, a baffle plate is arranged between each square groove, a plurality of rectangular grooves are arranged in the outer frame and positioned between the two square grooves, the length of each rectangular groove is the sum of the lengths of the three square grooves, a handle is arranged on the right side of the outer frame, the technical field of nucleic acid extraction is related, the multi-sample nucleic acid extraction device can increase the extraction efficiency by 3 times by using the arranged 96-hole deep hole plate, the quantity of nucleic acid extracted at one time can reach 600ul-750ul by using the arranged square grooves and the square grooves, when the sample volume required to be detected is larger, thereby improving the whole extraction efficiency, and the arranged square grooves and the rectangular grooves are used for saving washing and eluting the single plate, time is saved.

Description

Many sample sizes nucleic acid extraction element

Technical Field

The utility model relates to a nucleic acid extraction technical field specifically is a many sample size nucleic acid extraction element.

Background

The process of extracting nucleic acid by the magnetic bead method mainly comprises four steps of cracking, combining, washing and eluting, and nucleic acid extractors on the market at present mainly comprise 32 flux and 96 flux, wherein 32 flux can extract 32 samples at a time, 96 flux can extract 96 samples at a time, and 32 flux is more favored by customers due to small volume and low price in terms of market acceptance. The kit used in the 32-flux nucleic acid extractor is a rectangular kit with 8 rows by 12 columns of holes, when in use, the lysis and the combination are carried out in the 1 st column, the combination is carried out in the 2, 3 and 4 columns, the 5 th column is used for removing magnetic beads, the 6 th column is used for elution, the 7-12 columns and the like, one hole of the kit can contain at most 200 and 250ul of samples, when the amount of the samples required for detection exceeds 250ul, the 2 holes are used for extracting the samples, and thus the extraction efficiency is reduced invisibly.

When the existing device is used, the four steps of cracking, combining, washing and eluting are respectively carried out at different places, so that long time is wasted, and secondly, the nucleic acid is extracted step by step due to the small number of extraction when the device is used, so that the extraction efficiency of the nucleic acid is reduced.

SUMMERY OF THE UTILITY MODEL

To prior art not enough, the utility model provides a many sample size nucleic acid extraction element has solved the extraction substep operation to nucleic acid comparatively troublesome, and the quantity of once only extracting nucleic acid is less, influences the problem of the efficiency of nucleic acid extraction.

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: a multi-sample-size nucleic acid extraction device comprises a base, wherein two identical placing grooves are formed in the top of the front side of the base, a 96-hole deep pore plate is movably connected in each of the two placing grooves, each 96-hole deep pore plate comprises an outer frame, a plurality of identical square grooves are formed in two sides of an inner cavity of each outer frame, a partition board is arranged between each square groove for blocking, a plurality of rectangular grooves are formed in the inner part of each outer frame and located between the two square grooves, the length of each rectangular groove is the sum of the lengths of the three square grooves, and a handle is formed in the right side of each outer frame;

the rear side fixedly connected with actuating mechanism at base top, actuating mechanism front side just is located and installs bar magnet subassembly and bar magnet cover frame subassembly from the top down in proper order directly over the 96 hole deep hole board, and actuating mechanism can drive the whole translation of bar magnet subassembly and bar magnet cover frame subassembly and vertically go up and down respectively.

Preferably, the driving mechanism comprises a first slideway fixedly connected with the top of the rear side of the base, the first slideway is connected with a sliding seat in a sliding manner, and the top of the sliding seat is fixedly connected with a supporting arm.

Preferably, the bottom of the rear side of the base is fixedly connected with an X-axis motor, a first synchronous belt mechanism is connected between an output shaft of the X-axis motor and the top of the base, and one side of a transmission belt of the first synchronous belt mechanism is fixedly connected with the supporting arm.

Preferably, the right side fixedly connected with Z axle motor at support arm top, be connected with second hold-in range mechanism between the output shaft of Z axle motor and the left side of sliding seat, and drive belt one side fixedly connected with lift frame of second hold-in range mechanism, the top fixedly connected with vibrating motor of lift frame, there is linear guide in the inside of lift frame through two sliding connection of slide, and vibrating motor's output and linear guide's top fixed connection.

Preferably, the magnetic rod assembly comprises a first support frame fixedly connected to the right side of the lifting frame, and the magnetic rod sleeve frame assembly comprises a second support frame fixedly connected to the right side of the slider on the surface of the linear guide rail and located right above the first support frame.

Preferably, the front side of the second support frame is fixedly connected with a first placing frame, a plurality of magnetic rods are fixedly connected to the lower portion of the first placing frame, the front side of the second support frame is fixedly connected with a second placing frame, a plurality of magnetic force jackets are fixedly connected to the bottom of the second placing frame, and a plurality of magnetic beads are arranged inside the magnetic force jackets.

Preferably, the two sides of the bottom of the base are fixedly connected with connecting plates, and the connecting plates are fixedly connected with the front side and the rear side through limiting holes formed in the bolts.

Advantageous effects

The utility model provides a many sample volume nucleic acid extraction element. Compared with the prior art, the method has the following beneficial effects:

1. the utility model provides a many sample size nucleic acid extraction element, through the 96 hole deep hole boards that set up, the quantity that utilizes square groove and square groove that set up can once only draw nucleic acid can reach 600ul-750ul, the volume of the sample of single extraction is original 3 times, when the sample volume that requires to detect is great, can increase the efficiency of extracting 3 times, thereby the holistic efficiency of extraction has been improved, the square groove and the rectangular channel that utilize to set up save singly wash and elute it, time saving.

2. The utility model provides a many sample size nucleic acid extraction element, through Z axle motor and the X axle motor that sets up, can remove its four directions of controlling from top to bottom at the in-process of drawing to improve holistic extraction efficiency, utilize the vibrating motor that sets up to vibrate the nucleic acid test tube, thereby accelerate the crushing speed of nucleic acid.

Drawings

Fig. 1 is a front perspective view of the present invention;

fig. 2 is a rear perspective view of the present invention;

fig. 3 is a top view of the 96-well deep-hole plate of the present invention;

fig. 4 is a side sectional view of the driving mechanism of the present invention.

In the figure: 1. a base; 2. a first slideway; 3. a support arm; 4. an X-axis motor; 5. a lifting frame; 6. a second synchronous belt mechanism; 7. a first synchronous belt mechanism; 8. a sliding seat; 9. a Z-axis motor; 10. a linear guide rail; 11. a first support frame; 12. a vibration motor; 13. a second support frame; 14. placing a first rack; 15. a magnetic bar; 16. placing a second rack; 17. a magnetic jacket; 18. a 96-well deep-well plate; 181. an outer frame; 182. a square groove; 183. a rectangular groove; 184. a handle; 19. a placement groove; 20. a connecting plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: a multi-sample nucleic acid extraction device comprises a base 1, a driving mechanism comprises a first slideway 2 fixedly connected with the top of the rear side of the base 1, a sliding seat 8 is connected on the first slideway 2 in a sliding way, a supporting arm 3 is fixedly connected with the top of the sliding seat 8, two identical placing grooves 19 are arranged on the top of the front side of the base 1, a 96-hole deep hole plate 18 is movably connected in each placing groove 19, the 96-hole deep hole plate 18 comprises an outer frame 181, a plurality of identical square grooves 182 are arranged on two sides of an inner cavity of the outer frame 181, a partition board is arranged between each square groove 182 for blocking, a plurality of rectangular grooves 183 are arranged in the outer frame 181 and positioned between the two square grooves 182, the length of each rectangular groove 183 is the sum of the lengths of the three square grooves 182, a handle 184 is arranged on the right side of the outer frame 181, an X-axis motor 4 fixedly connected with the bottom of the rear side of the base 1, a first synchronous belt mechanism 7 is connected between the output shaft of the X-axis motor 4 and the top of the base 1, one side of a transmission belt of the first synchronous belt mechanism 7 is fixedly connected with the supporting arm 3, the rear side of the top of the base (1) is fixedly connected with a driving mechanism, the front side of the driving mechanism is sequentially provided with a magnetic rod assembly and a magnetic rod sleeve frame assembly from top to bottom right above the 96-hole deep pore plate (18), the driving mechanism can drive the magnetic rod assembly and the magnetic rod sleeve frame assembly to integrally translate and longitudinally and respectively lift, the right side of the top of the supporting arm 3 is fixedly connected with a Z-axis motor 9, a second synchronous belt mechanism 6 is connected between an output shaft of the Z-axis motor 9 and the left side of the sliding seat 8, the magnetic rod assembly and the magnetic rod sleeve frame assembly can move in the vertical and horizontal directions in the extraction process through the arranged Z-axis motor 12 and the arranged X-axis motor 4, so that the whole extraction efficiency is improved, the arranged vibrating motor 9 can vibrate the nucleic acid test tube so as to accelerate the crushing speed of the nucleic acid, a lifting frame 5 is fixedly connected to one side of a transmission belt of a second synchronous belt mechanism 6, a vibration motor 12 is fixedly connected to the top of the lifting frame 5, a linear guide rail 10 is slidably connected to the inside of the lifting frame 5 through a second slide rail, the output end of the vibration motor 12 is fixedly connected to the top of the linear guide rail 10, a magnetic bar assembly comprises a first support frame 11 fixedly connected to the right side of the lifting frame 5, a second support frame 13 fixedly connected to the right side of a surface sliding block of the linear guide rail 10 and located right above the first support frame 11, a first placing frame 14 is fixedly connected to the front side of the second support frame 13, a plurality of magnetic bars 15 are fixedly connected to the lower side of the first placing frame 14, a second placing frame 16 is fixedly connected to the front side of the first support frame 11, a plurality of magnetic force outer sleeves 17 are fixedly connected to the bottom of the second placing frame 16, a plurality of magnetic beads are arranged inside the magnetic force outer sleeves 17, connecting plates 20 are fixedly connected to both sides of the bottom of the base 1, and the connecting plate 20 is offered spacing hole through the bolt with both sides around and carries out fixed connection, through the 96 hole deep hole board 18 that sets up, the quantity that utilizes square groove 182 and square groove 182 that set up can be with once only extracting nucleic acid can reach 600ul-750ul, the volume of the sample of single extraction is original 3 times, when the sample volume that requires to detect is great, can increase the efficiency of extracting 3 times, thereby whole extraction efficiency has been improved, square groove 182 and the rectangular groove 183 that utilize to set up save singly to wash and elute it, save time.

When the device is used, the base 1 is installed by the connecting plate 20, a sample is placed on the outer frame 181, the Z-axis motor 9 is started to enable the second placing frame 16 to descend, the vibration motor 12 is started to drive the linear guide rail 10 to move up and down to drive the lifting frame 5 to move up and down, the first placing frame 14 is further driven to move up and down to complete the step-by-step up-and-down movement operation of the first placing frame 14 and the second placing frame 16, the magnetic outer sleeve 17 descends to the inside of the 96-hole deep pore plate 18 and contacts with the sample, the Z-axis motor 9 is used to drive the second placing frame 16 and the magnetic outer sleeve 17 to move up and down to crush the sample, the Z-axis motor 9 is used to drive the second placing frame 13 and the first placing frame 14 to descend, the magnetic rod 15 descends to the inside of the magnetic outer sleeve 17, the Z-axis motor 12 is started to drive the vibration of the magnetic outer sleeve 17 to crush the sample in the 96-hole deep pore plate 18, after the crushing is completed, the Z-axis motor 9 is started again to drive the first support frame 11 and the second slide rail 10 to ascend, the magnetic jacket 17 is moved into the rectangular groove 183, then the X-axis motor 4 is started, and the synchronizing wheel 6, the transmission belt 7 and the sliding seat 8 are utilized to drive the magnetic jacket 17 to move left and right, so that the cracking and the combination of the crushed samples are completed.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A multiple sample volume nucleic acid extraction device, includes base (1), its characterized in that: the novel portable multifunctional portable electronic device is characterized in that two identical placing grooves (19) are formed in the top of the front side of the base (1), a 96-hole deep hole plate (18) is movably connected in each of the two placing grooves (19), each 96-hole deep hole plate (18) comprises an outer frame (181), a plurality of identical square grooves (182) are formed in two sides of an inner cavity of each outer frame (181), a partition board is arranged between every two square grooves (182), a plurality of rectangular grooves (183) are formed in each outer frame (181) and located between every two square grooves (182), the length of each rectangular groove (183) is equal to the sum of the lengths of the three square grooves (182), and a handle (184) is formed in the right side of each outer frame (181);

the rear side fixedly connected with actuating mechanism at base (1) top, actuating mechanism front side just is located and installs bar magnet subassembly and bar magnet cover frame subassembly from the top down in proper order directly over 96 hole deep hole board (18), and actuating mechanism can drive the whole translation of bar magnet subassembly and bar magnet cover frame subassembly and vertically go up and down respectively.

2. The multiple-sample-size nucleic acid extraction device according to claim 1, wherein: the driving mechanism comprises a first slide way (2) fixedly connected with the top of the rear side of the base (1), a sliding seat (8) is connected onto the first slide way (2) in a sliding mode, and a supporting arm (3) is fixedly connected with the top of the sliding seat (8).

3. The multiple-sample-size nucleic acid extraction device according to claim 1, wherein: the X-axis motor (4) is fixedly connected with the bottom of the rear side of the base (1), a first synchronous belt mechanism (7) is connected between an output shaft of the X-axis motor (4) and the top of the base (1), and one side of a transmission belt of the first synchronous belt mechanism (7) is fixedly connected with the supporting arm (3).

4. The multiple-sample-size nucleic acid extraction device according to claim 2, wherein: the right side fixedly connected with Z axle motor (9) at support arm (3) top, be connected with second hold-in range mechanism (6) between the output shaft of Z axle motor (9) and the left side of sliding seat (8), and drive belt one side fixedly connected with lifting frame (5) of second hold-in range mechanism (6), top fixedly connected with vibrating motor (12) of lifting frame (5), there are linear guide (10) in the inside of lifting frame (5) through two sliding connection of slide, and the top fixed connection of the output of vibrating motor (12) and linear guide (10).

5. The apparatus for extracting nucleic acid with multiple sample sizes according to claim 4, wherein: the magnetic rod assembly comprises a first support frame (11) fixedly connected with the right side of the lifting frame (5), and the magnetic rod sleeve frame assembly comprises a second support frame (13) fixedly connected with the right side of the surface sliding block of the linear guide rail (10) and located right above the first support frame (11).

6. The apparatus for extracting nucleic acid with multiple sample volumes according to claim 5, wherein: the utility model discloses a magnetic field strength testing device, including support frame two (13), the front side fixedly connected with rack (14) of support frame two (13), a plurality of bar magnets (15) of below fixedly connected with of rack (14), support frame one (11) front side fixedly connected with rack two (16), a plurality of magnetic force overcoat (17) of bottom fixedly connected with of rack two (16), the inside of magnetic force overcoat (17) is provided with a plurality of magnetic beads.

7. The multiple-sample-size nucleic acid extraction device according to claim 1, wherein: the base is characterized in that connecting plates (20) are fixedly connected to the two sides of the bottom of the base (1), and limiting holes are formed in the front side and the rear side of each connecting plate (20) through bolts for fixed connection.