CN216434134U

CN216434134U - Rapid detection kit

Info

Publication number: CN216434134U
Application number: CN202122232829.2U
Authority: CN
Inventors: 赖鹏英; 杨海霞; 周波; 付亚成; 刘丽霞
Original assignee: Kefu Medical Technology Co ltd
Current assignee: Kefu Medical Technology Co ltd
Priority date: 2021-09-15
Filing date: 2021-09-15
Publication date: 2022-05-03
Anticipated expiration: 2031-09-15

Abstract

The utility model relates to a rapid detection kit, which comprises an upper cover, a lower cover and test paper positioned between the upper cover and the lower cover, wherein the upper cover is provided with a sample adding hole, the upper cover is provided with a mounting hole adjacent to the sample adding hole, a sample diluent storage device capable of moving at least downwards is movably arranged in the mounting hole, and the sample diluent storage device comprises a mounting piece movably arranged in the mounting hole and a storage piece which is connected below the mounting piece and is internally provided with a storage cavity; a puncture piece is arranged on the lower cover below the sample diluent storage device; the lower cover is also provided with a supporting platform protruding towards the sample diluent storage device, and the supporting platform is positioned below the storage piece and extrudes the sample diluent storage device when the sample diluent storage device moves downwards under the action of external force; an isolation mechanism which can separate or contact the puncture piece and the sample diluent storage device is movably arranged between the puncture piece and the sample diluent storage device. The utility model provides a unify the kit with independent detection card, sample diluent dropping bottle, colour comparison card originally, saved the operating procedure of dropwise add diluent, made things convenient for the measurement personnel judged result, let the detection flow more simple, smooth and easy.

Description

Rapid detection kit

Technical Field

The utility model relates to an external diagnosis detects technical field, in particular to short-term test kit.

Background

The novel coronavirus pneumonia (Corona Virus Disease 2019, COVID-19) caused by 2019-nCoV (2019 novel coronavirus) forms a huge threat to the public health system, the life health safety of people and the national economic development of China and even the whole world. Prevention of new crown infections requires successful induction of neutralizing antibodies (protective antibodies) by vaccination. The current methods for detecting neutralizing antibodies against novel coronaviruses are mainly live virus or pseudovirus neutralization assays. Due to the complex cell experiment process and the limitation of factors such as safety, reagent consumables and the like, the existing method is not suitable for high-throughput large-scale screening work of the new coronavirus neutralizing antibody. Antibody detection is particularly important in the evaluation of new corona vaccines and in the study of antiviral mechanisms.

The new crown antibody and neutralizing antibody detecting box in the current market mostly comprises independent sample diluent (dropper), dropper, neutralizing antibody colorimetric card and test paper detecting card. The general operation process is to take out the detection card and keep flat, then to drip serum/plasma or whole blood in the sample adding hole, finally to drip the sample diluent vertically, wait for time to read the result, and the neutralization antibody detection needs to compare the result with the colorimetric card. The number of accessories is large, and the accessories are independent from each other, so that the operation process of detection is complicated.

SUMMERY OF THE UTILITY MODEL

Based on this, the utility model provides a short-term test kit, it can save the operating procedure, makes things convenient for the measurement personnel to operate.

The utility model adopts the technical scheme as follows:

a rapid detection kit comprises an upper cover, a lower cover and test paper positioned between the upper cover and the lower cover, wherein a sample adding hole is formed in the upper cover, a mounting hole is formed in the upper cover and is close to the sample adding hole, a sample diluent storage device capable of moving at least downwards is movably arranged in the mounting hole, and the sample diluent storage device comprises a mounting piece movably arranged in the mounting hole and a storage piece which is connected below the mounting piece and is internally provided with a storage cavity; a puncture piece is arranged on the lower cover below the sample diluent storage device; the lower cover is also provided with a supporting platform protruding towards the sample diluent storage device, and the supporting platform is positioned below the storage piece and extrudes the sample diluent storage device when the sample diluent storage device moves downwards under the action of external force; an isolation mechanism which can separate or contact the puncture piece and the sample diluent storage device is movably arranged between the puncture piece and the sample diluent storage device.

Further, the top end of the mounting hole does not exceed the top end of the sampling hole; at least the bottom of the storage member is adapted to be pierced by the piercing member.

As a further improvement of the above embodiment, the mounting member includes a horizontal cover portion, a straight cylinder portion extending downward from the periphery of the cover portion, and a positioning portion extending horizontally outward from the outer side of the straight cylinder portion, the straight cylinder portion is located in the mounting hole, the positioning portion abuts against the lower surface of the upper cover around the mounting hole, and the straight cylinder portion and the cover portion enclose an accommodating portion recessed upward; the storage piece is a cylindrical soft capsule fixed in the containing part, the sample diluent is stored in the soft capsule, and the lower end of the sample diluent is lower than the positioning part.

As another improvement of the above embodiment, the mounting member includes a horizontal cover portion, a straight cylinder portion extending downward from the periphery of the cover portion, and a positioning portion extending horizontally outward from the outer side of the straight cylinder portion, the cover portion protrudes from the upper surface of the upper cover, the straight cylinder portion is located in the mounting hole, and the positioning portion abuts against the lower surface of the upper cover located around the mounting hole; the storage piece is connected in the cylinder of straight section of thick bamboo portion below as an organic whole, and the bottom of cylinder is equipped with sealing film, and the inside sample diluent that stores of cylinder.

Furthermore, a notch penetrating through the upper surface of the support table is formed in the support table, the puncturing piece is arranged in the notch, and the notch is horizontally recessed from the outer periphery of the support table close to the test paper side to the center of the support table; the lower cover is provided with a positioning groove for installing the test paper, the positioning groove comprises a positioning lug located at the tail end of the test paper, the positioning lug is located in the notch, and the puncturing piece is located in the positioning lug and punctures the tail end of the test paper.

Furthermore, the upper surface of the support platform is inclined, and the inclination direction of the support platform is inclined downwards from one end far away from the test paper to one end close to the test paper.

As a further improvement of the above embodiment, the height of the piercing member is not less than the lowest height of the support table and not more than the highest height of the support table.

As a further improvement of the above embodiment, the mounting member includes a horizontal cover portion, a straight cylinder portion extending downward from the periphery of the cover portion, and a positioning portion extending horizontally outward from the outer side of the straight cylinder portion, the straight cylinder portion is located in the mounting hole, the positioning portion abuts against the lower surface of the upper cover around the mounting hole, the straight cylinder portion and the cover portion enclose an accommodating portion recessed upward, and one side of the straight cylinder portion close to the test paper protrudes downward for a length exceeding one side of the straight cylinder portion away from the test paper; wherein, the storage member is a cylindrical soft capsule fixed in the containing part, the sample diluent is stored in the soft capsule, and the lower end of the sample diluent is lower than the positioning part.

As a further improvement of the above embodiment, the isolation mechanism includes a baffle plate, one end of which is disposed between the puncturing member and the sample diluent storage device to isolate the puncturing member from the sample diluent storage device, and the other end of which is exposed through a through hole disposed on the upper cover or the lower cover; or the isolation mechanism comprises a spring sleeved on the puncturing piece, and the upper end of the spring is higher than the top end of the puncturing piece when the spring is in an extension state.

Furthermore, a neutralizing antibody test paper and an IgG/IgM test paper are arranged between the upper cover and the lower cover, and the upper cover is respectively provided with a corresponding sample adding hole, a sample diluent storage device, a puncturing piece, an isolating mechanism and a supporting table corresponding to the two test papers; the upper cover is also provided with observation holes corresponding to the two test papers respectively, and one side of the upper cover on the observation holes is provided with a colorimetric area.

Compared with the prior art, the utility model discloses following beneficial effect has:

the utility model discloses a rapid detection kit, through installing the most partial sample diluent storage device that divides in the market integratively in the upper cover, reduced the required independent accessory of detection; the sample diluent storage device is movably arranged close to the sample adding hole, and the lower cover is provided with the puncturing piece, so that when the sample diluent storage device moves downwards under the action of external force, the puncturing piece can puncture the bottom of the sample diluent storage device, the sample diluent in the sample diluent storage device is released to flow onto the test paper to be combined with the sample added dropwise from the sample adding hole, and the operation step of adding the sample diluent dropwise in the prior art is saved; the puncture piece is positioned below the sample diluent storage device, and is hidden in the whole kit without being exposed, so that the kit is safer and more reliable. The utility model discloses a short-term test kit can let the detection flow more simple, smooth and easy.

Drawings

The foregoing and other objects, features and advantages of the invention will be apparent from the following more particular description of preferred embodiments of the invention, as illustrated in the accompanying drawings. Like reference numerals refer to like parts throughout the drawings, and the drawings are not intended to be drawn to scale in actual dimensions, emphasis instead being placed upon illustrating the principles of the invention.

FIG. 1 is a perspective view of the rapid test kit of the present invention;

FIG. 2 is a schematic view of the cover of FIG. 1 after opening and with one of the shutters retracted;

FIG. 3 is a schematic cross-sectional view of the rapid test kit of FIG. 1;

FIG. 4 is a schematic view of the rapid test kit according to the present invention, in which the baffle is not pulled away and the piercing member is not activated;

FIG. 5 is a schematic view of the puncturing element of FIG. 4 after the flapper has been withdrawn;

FIG. 6 is a schematic structural diagram of the test strip.

Description of reference numerals:

1. an upper cover; 10. an observation hole; 11. a sample application hole; 12. mounting holes; 13. a colorimetric area;

2. a lower cover; 20. a piercing member; 21. a support table; 210. a notch; 22. positioning a groove; 220. positioning the bump;

3. test paper; 30. PVC board; 31. a sample pad; 32. a colloidal gold bonding pad; 33. NC film; 34. a water absorbent pad;

4. a sample diluent storage; 40. a mounting member; 401. a cover portion; 402. a straight tube portion; 403. a positioning part; 41. a storage member;

5. and a baffle plate.

Detailed Description

In order to facilitate understanding of the present invention, the present invention will be described more fully hereinafter with reference to the accompanying drawings.

It will be understood that when an element is referred to as being "connected" to another element, it can be directly connected to the other element and be integral therewith, or intervening elements may also be present. The terms "mounted," "one end," "the other end," and the like are used herein for illustrative purposes only. The terms "upper", "lower", "horizontal", "vertical", and the like indicate orientations or positional relationships based on directions or positional relationships shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have the specified orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

Referring to fig. 1, the rapid detection kit of the present invention is specifically a novel coronavirus IgG/IgM and neutralizing antibody combined detection kit in the preferred embodiment.

Referring to fig. 1 and fig. 2, the novel coronavirus IgG/IgM and neutralizing antibody joint detection kit of the present invention comprises an upper cover 1, a lower cover 2, and two kinds of test paper 3 located between the upper cover 1 and the lower cover 2 and respectively used for detecting neutralizing antibodies and IgG/IgM antibodies. The neutralizing antibody test paper is used for detecting novel coronavirus neutralizing antibodies, and the IgG/IgM test paper is used for detecting novel coronavirus IgG/IgM antibodies.

Referring to fig. 6, the two types of test paper 3 have substantially the same structure, and each test paper includes a PVC plate 30, and a sample pad 31, a gold colloidal conjugate pad 32, an NC membrane 33, and a water absorption pad 34 are sequentially disposed on the PVC plate 30 in the chromatography direction. The difference lies in that: a detection area of an NC film 33 of the neutralizing antibody test paper 3 is coated with human ACE-2 protein, a quality control area is coated with goat anti-chicken IgY antibody, and a binding pad is sprayed with SARS-CoV-2 antigen and chicken IgY antibody which are respectively marked by colloidal gold; the colloidal gold combined pad 32 of the IgG/IgM test paper 3 contains SARS-CoV-2 recombinant antigen labeled with colloidal gold and chicken IgY, and the NC membrane 33 is provided with an IgM detection line, an IgG detection line and a quality control line C, wherein the IgM detection line is coated with mouse anti-human IgM, the IgG detection line is coated with mouse anti-human IgG, and the quality control line is coated with goat anti-chicken IgY.

With continued reference to fig. 1 to 3, the upper cover 1 is respectively provided with an observation hole 10, a sampling hole 11 and a mounting hole 12 in sequence along the length direction of the test paper 3 corresponding to the two types of test paper 3, and the mounting hole 12 is provided adjacent to the sampling hole 11. The observation hole 10 corresponds to the position of the NC film 33 of the test paper 3 so as to observe the reaction of the test paper 3. The sample adding hole 11 corresponds to the position of the sample pad 31 of the test paper 3, and is convenient for dropwise adding the sample to the sample pad 31. And the mounting hole 12 corresponds to the end position of the sample pad 31. The top end of the mounting hole 12 does not exceed the top end of the sampling hole 11, so that the hole on the upper cover 1 can be prevented from protruding, and the uniformity and the attractiveness of the appearance of the kit can be kept.

The upper cover 1 is provided with a colorimetric area 13 on the side of the observation hole 10 corresponding to the neutralizing antibody test paper. Specifically, the colorimetric region 13 includes a plurality of color patches provided on the upper surface of the upper cover 1 and marks located beside the color patches. Through unified the setting on the upper cover 1 of kit with independent colour chip originally, saved the accessory, made things convenient for measurement personnel judgement result, let the detection flow more simple, smooth and easy.

Referring to fig. 2 to 4, further, each of the mounting holes 12 is movably provided with a sample diluent storage means 4 at least downwardly movable at a position corresponding to the end of the sample pad 31, so that the sample diluent storage means 4 corresponds to the end of the sample pad 31 and ensures that the sample diluent can flow onto the end of the sample pad 31.

Further, the sample diluent storage device 4 comprises a mounting member 40 movably mounted in the mounting hole 12 and a storage member 41 connected below the mounting member 40 and having a storage chamber therein, wherein at least the bottom of the storage member 41 is penetrable by the puncturing member 20. When the sample diluent storage device 4 moves downwards under the action of external force, the puncturing part 20 punctures the bottom of the sample diluent storage device 4, so that the sample diluent in the sample diluent storage device is released to flow onto the test paper 3 and is combined with the sample dripped from the sample adding hole 11, and the operation step of dripping the sample diluent in the past is saved.

With reference to fig. 4 and 5, in the preferred embodiment, the mounting member 40 includes a horizontal cover portion 401, a straight tube portion 402 extending downward from the periphery of the cover portion 401, and a positioning portion 403 extending horizontally outward from the outer side of the straight tube portion 402, the straight tube portion 402 is located in the mounting hole 12, and the positioning portion 403 abuts against the lower surface of the upper cover 1 around the mounting hole 12. The straight cylinder portion 402 and the cover portion 401 enclose an accommodating portion that is recessed upward, in other words, an opening of the accommodating portion is downward. The mounting member 40 with such a structure is convenient to be buckled and mounted in the mounting hole 12, the positioning portion 403 is abutted against the lower surface of the upper cover 1 upwards to realize positioning, the mounting is convenient, and the sample diluent storage device 4 can be pressed down to be contacted with the puncturing member 20 and be punctured by only pressing the cover portion 401. Meanwhile, after installation, the cover part 401 slightly protrudes from the upper surface of the upper cover 1, and the whole sample diluent storage device 4 is basically accommodated in the reagent kit, so that the surface of the upper cover 1 of the reagent kit is smooth and beautiful and is not obtrusive. In the preferred embodiment, the side of the straight cylinder 402 close to the test paper 3 protrudes downwards for a length longer than the side of the straight cylinder 402 far from the test paper 3, i.e. the bottom end of the straight cylinder 402 on the right side is lower than the bottom end of the straight cylinder on the left side in fig. 4, so that the straight cylinder 402 forms a block on the right side of the storage member 41.

In the preferred embodiment, the storage member 41 is a cylindrical soft capsule fixed in the containing portion, and the sample diluent is stored in the soft capsule, and the lower end of the soft capsule is lower than the positioning portion 403. The present embodiment stores the sample diluent (similar to the cod liver oil soft capsule) by a cylindrical soft capsule, and since the soft capsule itself is flexibly deformable, the soft capsule can be inserted upward into the accommodating portion of the mounting member 40 to be fixed, and can be easily punctured.

In another embodiment, not shown, the mounting member 40 includes a horizontal cover portion 401, a straight tube portion 402 extending downward from the periphery of the cover portion 401, and a positioning portion 403 extending horizontally outward from the outside of the straight tube portion 402, the straight tube portion 402 is located in the mounting hole 12, and the positioning portion 403 abuts against the lower surface of the upper cover 1 around the mounting hole 12. The storage member 41 is integrally connected to a cylinder below the cylindrical portion 402, a sealing film is provided at the bottom of the cylinder to facilitate puncturing, and a sample diluent is stored in the cylinder. Of course, the present invention is not limited thereto, as long as the sample diluent storage device 4 can be buckled into the mounting hole 12 of the upper cover 1 and the bottom thereof can be easily punctured.

In the preferred embodiment, the lower cap 2 is provided with a puncturing member 20 at a position below the sample diluent storage device 4, and the puncturing member 20 is a sharp spike for puncturing the bottom of the sample diluent storage device 4 to release the sample diluent. Specifically, a puncture piece 20 is provided below each sample diluent storage device 4. The puncturing member 20 is located below the sample diluent storage 4, and is hidden inside the entire reagent cartridge without being exposed, thereby being safer and more reliable.

The lower cover 2 is further provided with a support table 21 protruding toward the sample diluent storage device 4, and the support table 21 is located below the storage member 41 and presses the sample diluent storage device 4 when the sample diluent storage device 4 moves downward by an external force. An isolation mechanism is also movably disposed between the puncturing member 20 and the sample diluent storage device 4 to separate or contact the puncturing member and the sample diluent storage device.

In the preferred embodiment, the isolation mechanism includes a baffle 5, one end of the baffle 5 is disposed between the puncturing member 20 and the sample diluent storage device 4 to separate them, and the other end of the baffle 5 passes through a through hole disposed on the upper cover 1 or the lower cover 2 and is exposed to form a portion for holding by the testing personnel. Can conveniently separate piercing member 20 and sample diluent storage device 4 through baffle 5, sample diluent device is punctured by mistake and takes place to reveal when avoiding non-measuring. When the detection is carried out, the puncture can be realized only by pulling out the baffle 5 and then pressing down the sample diluent storage device 4, and the operation is very simple and convenient.

In other embodiments, not shown, the isolation mechanism may also include a spring disposed about the puncturing member 20 and having an upper end that is higher than the upper end of the puncturing member 20 when the spring is in an extended state. The spring is compressed when sample diluent storage device 4 is pushed down, makes puncture 20 top contact and puncture sample diluent storage device 4 bottom, can realize equally the utility model discloses isolator mechanism's function.

In this embodiment, brace table 21 indirectly supports in sample diluent storage device 4's bottom through baffle 5, can guarantee the stable location behind sample diluent storage device 4 spiral-lock to mounting hole 12 in, can extrude sample diluent storage device 4 again when sample diluent storage device 4 moves down under the exogenic action, is favorable to the release of sample diluent.

Referring to fig. 2 to 4, a notch 210 is further formed on the supporting platform 21 and penetrates through the upper surface thereof, and the puncturing element 20 is disposed in the notch 210. The notch 210 is recessed horizontally from the outer periphery of the support table 21 on the side closer to the test paper 3 toward the center thereof. The lower cover 2 is provided with a positioning groove 22 for mounting the test paper 3, the positioning groove 22 includes a positioning protrusion 220 located at the end of the test paper 3, the positioning protrusion 220 is U-shaped, the positioning protrusion 220 is located in the notch 210, and the puncturing member 20 is located in the positioning protrusion 220 and punctures the end of the test paper 3. The design surrounds the support platform 21 at the end of the test paper 3, i.e. the end of the sample pad 31, and the puncturing element 20 is located at the end of the sample pad 31, so that the area of the sample diluent flowing to the test paper 3 can be increased, and the reliable sample dilution can be ensured.

Preferably, the upper surface of the support base 21 is inclined in a direction inclined downward from one end away from the test strip 3 to one end close to the test strip 3. With this arrangement, after puncturing the sample diluent storage device 4, the sample diluent will flow along the direction inclined from the upper surface of the supporting platform 21, i.e. the end where the test paper 3 is located, so as to ensure that the sample diluent flows to the end of the sample pad 31 of the test paper 3. In the preferred embodiment, the bottom end of the right side of the straight cylinder 402 is lower than the bottom end of the left side of the straight cylinder 402, so that the soft capsule is blocked by the right side of the straight cylinder 402, and when the soft capsule is extruded and deformed by the support table 21, the soft capsule can only bulge out to the positions of the puncture piece 20 and the sample pad 31 at the tail end of the test paper 3 in the lower right direction, and the sample diluent can be better guided to the test paper 3.

Preferably, the height of the puncturing member 20 is not less than the lowest height of the support platform 21 and not more than the highest height of the support platform 21. Ensuring that the sample diluent reservoir 4 is actually punctured when depressed while ensuring that the puncturing member 20 is not exposed.

In other embodiments, the positions of the mounting hole 12 and the sample diluent storage device 4 may be different from the positions of the observation hole 10 and the sample application hole 11 (i.e., not in the longitudinal direction of the test strip 3), and in the case of sufficient space, may be provided on one side in the longitudinal direction of the test strip 3, as long as the positions of the sample pad 31 next to the sample application hole 11 and next to the test strip 3 are satisfied.

The utility model provides a short-term test box, the sample diluent can not reveal under the circumstances of exerting powerless, through taking out from baffle 5, the lid 401 of lightly pressing sample diluent storage device 4 makes sample diluent storage device 4 position descend, puncture piece 20 on sample diluent storage device 4 bottom by lower cover 2 punctures, the release of sample diluent, combine and move forward with the sample under the effect of pad 34 absorbs water, get into observation hole 10 at last, appear the colour development or disappear the line reaction on the detection area.

Referring to fig. 4 and 5, the novel coronavirus IgG/IgM and neutralizing antibody joint detection kit of the present invention is used as follows:

1. taking out the rapid detection kit, horizontally placing, and dripping the collected sample serum, plasma or whole blood from the sample adding hole 11 by using a dropper;

2. withdrawing the baffle 5, slightly pressing the cover part 401 of the sample diluent storage device 4, puncturing the bottom of the sample diluent storage device 4 by using the puncturing part 20 protruding from the lower cover 2, and enabling the sample diluent to flow to the tail end of the sample pad 31 and be combined with the dripped sample under the action of the water absorption pad 34;

3. and (5) observing by eyes, and waiting for a result. On the premise that a colored strip appears on the quality control line, the colored strip appears on any detection line of the IgG/IgM test paper indicates that a detection substance corresponding to the detection line is positive. The neutralizing antibody test paper judges the condition of generating neutralizing antibodies by comparing a colorimetric area 13 beside a viewing hole 10, wherein the color is negative between G10-G8, weak positive between G7-G6, medium positive between G5-G4 and strong positive between G3-G1.

Compared with the prior art, the utility model provides a quick detect reagent box will originally independent detect card, sample diluent dropping bottle, color comparison card unify the kit, has saved the operating procedure of dropwise add diluent, has made things convenient for the measurement personnel judged result, lets the detection flow more simple, smooth and easy.

According to the innovative concept of the utility model, the rapid detection kit of the utility model can also be provided with other types of test paper for detecting different targets; and the method can also be used for single-joint detection, triple detection and even multi-joint detection.

The present invention is not limited thereto.

The above-mentioned embodiments only express the specific embodiments of the utility model, and the description thereof is specific and detailed, but not construed as limiting the scope of the utility model. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. A rapid detection kit comprises an upper cover (1), a lower cover (2) and test paper (3) positioned between the upper cover (1) and the lower cover (2), wherein a sample adding hole (11) is formed in the upper cover (1), and the rapid detection kit is characterized in that a mounting hole (12) is formed in the upper cover (1) and is adjacent to the sample adding hole (11), a sample diluent storage device (4) capable of moving at least downwards is movably mounted in the mounting hole (12), and the sample diluent storage device (4) comprises a mounting piece (40) movably mounted in the mounting hole (12) and a storage piece (41) connected below the mounting piece (40) and internally provided with a storage cavity; a puncture piece (20) is arranged on the lower cover (2) below the sample diluent storage device (4); the lower cover (2) is also provided with a supporting platform (21) protruding towards the sample diluent storage device (4), and the supporting platform (21) is positioned below the storage piece (41) and extrudes the sample diluent storage device (4) when the sample diluent storage device (4) moves downwards under the action of external force; an isolation mechanism which can separate or contact the puncture piece (20) and the sample diluent storage device (4) is movably arranged between the puncture piece and the sample diluent storage device.

2. The rapid test kit according to claim 1,

the top end of the mounting hole (12) does not exceed the top end of the sampling hole (11);

at least the bottom of the reservoir (41) is pierceable by the piercing member (20).

3. The rapid test kit according to claim 2,

the mounting piece (40) comprises a horizontal cover part (401), a straight cylinder part (402) extending downwards from the periphery of the cover part (401), and a positioning part (403) extending outwards and horizontally from the outer side of the straight cylinder part (402), wherein the straight cylinder part (402) is positioned in the mounting hole (12), the positioning part (403) is abutted against the lower surface of the upper cover (1) positioned around the mounting hole (12), and the straight cylinder part (402) and the cover part (401) enclose an accommodating part which is sunken upwards;

the storage piece (41) is a cylindrical soft capsule fixed in the containing part, sample diluent is stored in the soft capsule, and the lower end of the soft capsule is lower than the positioning part (403).

4. The rapid test kit according to claim 2,

the mounting piece (40) comprises a horizontal cover part (401), a straight cylinder part (402) extending downwards from the periphery of the cover part (401), and a positioning part (403) extending outwards and horizontally from the outer side of the straight cylinder part (402), wherein the cover part (401) protrudes out of the upper surface of the upper cover (1), the straight cylinder part (402) is positioned in the mounting hole (12), and the positioning part (403) is abutted to the lower surface of the upper cover (1) around the mounting hole (12);

the storage part (41) is a cylinder integrally connected to the lower part of the straight cylinder part (402), a sealing film is arranged at the bottom of the cylinder, and a sample diluent is stored in the cylinder.

5. The rapid detection kit according to claim 1, wherein the support platform (21) is formed with a notch (210) extending through an upper surface thereof, the puncturing member (20) is disposed in the notch (210),

the notch (210) is horizontally recessed from the outer periphery of the side, close to the test paper (3), of the support table (21) to the center of the notch;

the lower cover (2) is provided with a positioning groove (22) for installing the test paper (3), the positioning groove (22) comprises a positioning lug (220) located at the tail end of the test paper (3), the positioning lug (220) is located in the notch (210), and the puncturing piece (20) is located in the positioning lug (220) and punctures the tail end of the test paper (3).

6. The rapid detection kit according to claim 1, wherein the upper surface of the support platform (21) is inclined in a direction that is inclined downward from an end away from the test strip (3) to an end close to the test strip (3).

7. The rapid detection kit according to claim 6, wherein the height of the lancet (20) is not lower than the lowest height of the supporting platform (21) and not higher than the highest height of the supporting platform (21).

8. The rapid test kit according to claim 6,

the mounting piece (40) comprises a horizontal cover part (401), a straight cylinder part (402) extending downwards from the periphery of the cover part (401), and a positioning part (403) extending outwards and horizontally from the outer side of the straight cylinder part (402), the straight cylinder part (402) is positioned in the mounting hole (12), the positioning part (403) abuts against the lower surface of the upper cover (1) positioned around the mounting hole (12), the straight cylinder part (402) and the cover part (401) form an upwards-concave accommodating part, and one side of the straight cylinder part (402) close to the test paper (3) protrudes downwards for a length exceeding one side of the straight cylinder part (402) away from the test paper (3);

9. The rapid test kit according to claim 1,

the isolation mechanism comprises a baffle plate (5), one end of the baffle plate (5) is arranged between the puncture piece (20) and the sample diluent storage device (4) to separate the puncture piece from the sample diluent storage device, and the other end of the baffle plate (5) is exposed through a through hole arranged on the upper cover (1) or the lower cover (2); or

The isolation mechanism comprises a spring sleeved on the puncturing piece (20), and the upper end of the spring is higher than the top end of the puncturing piece (20) when the spring is in an extending state.

10. The rapid detection kit according to any one of claims 1 to 9,

a neutralizing antibody test paper (3) and an IgG/IgM test paper (3) are arranged between the upper cover (1) and the lower cover (2), and the upper cover (1) is respectively provided with a corresponding sample adding hole (11), a sample diluent storage device (4), a puncture piece (20), an isolation mechanism and a support table (21) corresponding to the two test papers (3);

the test paper box is characterized in that the upper cover (1) is further provided with observation holes (10) corresponding to the two test papers (3), and a color comparison area (13) is arranged on one side of the observation holes (10) on the upper cover (1).