CN216349779U - Collecting assembly for cell sap samples - Google Patents
Collecting assembly for cell sap samples Download PDFInfo
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- CN216349779U CN216349779U CN202122801811.XU CN202122801811U CN216349779U CN 216349779 U CN216349779 U CN 216349779U CN 202122801811 U CN202122801811 U CN 202122801811U CN 216349779 U CN216349779 U CN 216349779U
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Abstract
The utility model provides a collection assembly of a cell sap sample, comprising: the cell liquid sampling device comprises a collecting bottle and a filter cup used for taking a cell liquid sample from the collecting bottle; the collection bottle comprises a bottle body, a bottle cap and a sealing element; the bottle cap is arranged at the top end of the bottle body, and the sealing element is arranged at the top end of the bottle cap; the bottle cap comprises a cap body, wherein an annular bulge is arranged at the upper part of the cap body, and the annular bulge and the cap body form a liquid discharge groove; the size of the outer wall of the filter cup is matched with that of the annular bulge, and the filter cup is placed in the liquid discharge tank to take out a cell fluid sample; an air inlet hole for guiding air is formed in the cover body; the air inlet is arranged beside the annular bulge. The collection assembly for the cell sap sample has a simple integral structure, extracts the cell sap sample by adopting a negative pressure extraction mode, realizes non-contact extraction of the cell sap sample, avoids secondary pollution, simplifies operation steps and improves working efficiency.
Description
Technical Field
The utility model relates to the technical field of cell sample liquid collection and sheet preparation for a membrane type liquid-based cell sheet preparation machine, in particular to a cell sample collection assembly.
Background
The core of the membrane type liquid-based cell pelleter is a cell filtration transfer adsorption technology, and according to the principle of liquid-based cytology, the cell is adsorbed by adopting a physical suction and filter membrane technology and then transferred onto a glass slide to form a uniform cell thin layer on the glass slide, so that further dyeing and diagnosis are facilitated.
In the prior art, the bottle cap of the liquid storage bottle used by the membrane type liquid-based cell pelleter needs to be unscrewed manually when the liquid storage bottle is used, the human cell sample liquid is taken out of the liquid storage bottle through a dropper or other tools and put into the pelleter, the bottle cap is opened in the process, the secondary pollution is increased, the working steps of experimenters are increased, and the efficiency is reduced.
In view of the above, the present invention is particularly proposed.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a cell sap sample collecting assembly, which is matched with a collecting bottle through a filter cup, can directly extract a cell sap sample by using negative pressure without opening a bottle cap, realizes non-contact extraction of the cell sap sample, avoids secondary pollution, simplifies operation steps and improves working efficiency.
In order to achieve the above purpose of the present invention, the following technical solutions are adopted:
the utility model provides a collection assembly of a cell sap sample, comprising: the cell liquid sampling device comprises a collecting bottle and a filter cup used for taking a cell liquid sample from the collecting bottle;
the collection bottle comprises a bottle body, a bottle cap and a sealing element; the bottle cap is arranged at the top end of the bottle body, and the sealing element is arranged at the top end of the bottle cap;
the bottle cap comprises a cap body, wherein an annular bulge is arranged at the upper part of the cap body, and the annular bulge and the cap body form a liquid discharge groove; the size of the outer wall of the filter cup is matched with that of the annular bulge, and the filter cup is placed in the liquid discharge tank to take out a cell fluid sample; an air inlet hole for guiding air is formed in the cover body; the air inlet is arranged beside the annular bulge;
a guide pipe is arranged at the lower part of the cover body and is communicated with the liquid discharge groove so as to discharge the cell sap sample stored in the bottle body; and a filter screen for filtering the cell sap sample is arranged in the conduit.
In the prior art, the bottle cap of the liquid storage bottle used by the membrane type liquid-based cell pelleter needs to be unscrewed manually when the liquid storage bottle is used, the human cell sample liquid is taken out of the liquid storage bottle through a dropper or other tools and put into the pelleter, the bottle cap is opened in the process, the secondary pollution is increased, the working steps of experimenters are increased, and the efficiency is reduced.
In order to solve the technical problems, the utility model provides a cell sap sample collecting assembly, which is matched with a collecting bottle through a filter cup, can directly extract a cell sap sample by using negative pressure, does not need to open a bottle cap, realizes non-contact extraction of the cell sap sample, avoids secondary pollution, simplifies operation steps and improves working efficiency; the liquid discharge groove is arranged on the bottle cap, so that the filter cup is convenient to position and mount; through the arrangement of the air inlet hole, air can be guided in the bottle body when the cell fluid sample is extracted by the filter cup under negative pressure, so that the stable extraction process is ensured; the filter screen is arranged in the conduit, so that impurities and mucus in the cell sap can be conveniently filtered; the bottle cap can be sealed by arranging the sealing piece, so that the cell sap sample in the bottle body is prevented from being polluted.
In fact, when the cell fluid sample does not need to be extracted, the sealing element is sealed on the bottle cap, and the sealing element, the bottle cap and the bottle body form a closed environment, so that the cell fluid sample is prevented from being polluted; when the cell liquid sample extraction device is used, the sealing element is opened, the filter cup is placed in the liquid discharge tank, and negative pressure is used for extraction, so that cell liquid samples can be extracted.
Preferably, the bottom of the conduit extends downwards to form a connecting pipe, the inner diameter and the outer diameter of the connecting pipe are smaller than those of the conduit, and a step is formed between the outer part of the connecting pipe and the conduit. In fact, when the bottle cap is installed on the bottle body, the pipe structure formed by the conduit and the connecting pipe is directly communicated to the bottom of the bottle body.
Preferably, a water stopping ball is arranged in the conduit, and the diameter of the water stopping ball is larger than that of the connecting pipe and smaller than that of the conduit. The water stopping ball is arranged to prevent the cell sap sample from flowing backwards, when cell sap is extracted under negative pressure, the cell sap flows upwards, and the water stopping ball floats upwards; when the negative pressure is insufficient, the cell sap flows downwards, and the water stop ball is blocked at the outlet at the bottom of the catheter under the driving of the cell sap, so that the cell sap is prevented from flowing back.
Preferably, an arc-shaped stop block is arranged in the guide pipe and is positioned above the water stop ball; a spring is arranged between the arc-shaped stop block and the water stopping ball, and the water stopping ball is compressed by the spring in a natural state so that the outlet at the bottom of the conduit is blocked by the water stopping ball. On one hand, the water stopping ball can be limited, and the water stopping ball is prevented from flowing out of the top of the catheter; on the other hand, can prevent cell sap from accumulating in the catheter. In fact, the arc dog can set up a plurality ofly, and has certain space between each arc dog, like this, when negative pressure is great, stagnant water ball pressure spring, cell liquid sample can continue upwards flowing in the space, guarantees to draw the steady operation of work.
Preferably, the sealing member is a sealing film covering the top of the bottle cap. The sealing film can be a plastic film and is fixed on the bottle cap through hot melt adhesive; when the cell sap negative pressure extraction device is used, the sealing film is torn, the filter cup is placed in the liquid discharge tank, and then the cell sap sample can be extracted under the negative pressure.
Preferably, the sealing element comprises a plug body, a first sealing part matched with the liquid discharge groove is arranged at the bottom of the plug body, and a second sealing part matched with the air inlet hole is arranged on one side of the first sealing part. Compared with a sealing film, the plug body is better in sealing performance on the bottle body, and the cell liquid sample can be prevented from flowing onto the bottle cap from the air inlet hole when the bottle body is knocked down or inverted.
Preferably, a sealing ring is arranged at the bottom of the liquid discharge groove. Set up the sealing washer and can improve sealing performance, cell sap leakage along filtering cup and flowing back tank bottom when preventing the sample and drawing.
Preferably, the bottom of the filter cup is provided with a liquid inlet, the liquid inlet is provided with a filter membrane, and the filter membrane is provided with a plurality of filter holes for filtering cells. Furthermore, the aperture of the filtering hole is 8-10 μm. Actually, the diameter of the cell is about 30 μm basically, when the cell is extracted under negative pressure, water in the cell sap enters the filter cup through the filter membrane, the cell sample is adsorbed at the bottom of the filter membrane, and the bottom of the filter cup is dipped on the glass slide, so that the cell sample can be extracted into the glass slide.
Preferably, the filter screen is disposed at the top of the duct. Furthermore, the filter screen is 100-180 meshes, so that impurities and mucus can be well filtered.
Preferably, the bottle cap and the bottle body are fixed to each other through threaded connection. Set up the fastening nature like this and be convenient for the dismouting good.
Preferably, knurls are arranged on the outer side of the bottle cap and the outer side of the filter cup. The knurling is arranged to facilitate taking of a user and prevent falling off in the taking process.
Compared with the prior art, the utility model has the beneficial effects that:
(1) according to the collecting assembly for the cell fluid sample, the filter cup is matched with the collecting bottle, the cell fluid sample can be directly extracted by negative pressure without opening a bottle cap, the cell fluid sample is extracted in a non-contact manner, secondary pollution is avoided, the operation steps are simplified, and the working efficiency is improved;
(2) the liquid discharge groove is arranged on the bottle cap, so that the filter cup is convenient to position and mount; through the arrangement of the air inlet hole, air can be guided in the bottle body when the cell fluid sample is extracted by the filter cup under negative pressure, so that the stable extraction process is ensured;
(3) the filter screen is arranged in the conduit, so that impurities and mucus in the cell sap can be conveniently filtered;
(4) the bottle cap can be sealed by arranging the sealing piece, so that the cell sap sample in the bottle body is prevented from being polluted.
Drawings
Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the utility model. Also, like reference numerals are used to refer to like parts throughout the drawings. In the drawings:
FIG. 1 is a schematic structural diagram of a collection bottle according to an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a bottle cap according to an embodiment of the present invention;
FIG. 3 is a schematic structural diagram of a sealing member according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of an external structure of a collection bottle according to an embodiment of the present invention;
5-6 are external structural schematic views of a filter cup provided by an embodiment of the utility model;
fig. 7 is a schematic view of a cell fluid sample collection assembly according to an embodiment of the present invention.
Wherein:
10-a seal; 101-a plug body;
102-a second seal; 103-a first seal;
20-bottle cap; 201-a cover body;
202-an annular projection; 203-sealing ring;
204-air intake; 205-a catheter;
206-arc-shaped stop block; 207-a spring;
208-water stopping ball; 209-connecting pipe;
210-a filter screen; 30-bottle body;
40-a filter cup; 401-filtration membrane.
Detailed Description
The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings and the detailed description, but those skilled in the art will understand that the following described embodiments are some, not all, of the embodiments of the present invention, and are only used for illustrating the present invention, and should not be construed as limiting the scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In order to more clearly illustrate the technical solution of the present invention, the following description is made in the form of specific embodiments.
Examples
Referring to FIGS. 1-7, the present embodiment provides a collection assembly for a cytosol sample, comprising: a collection vial and a filter bowl 40 for removing a sample of the cellular fluid from the collection vial.
As shown in fig. 1, the collection bottle comprises a bottle body 30, a bottle cap 20 and a sealing member 10; the bottle cap 20 is disposed at the top end of the bottle body 30, and the sealing member 10 is disposed at the top end of the bottle cap 20.
As shown in fig. 2, the bottle cap 20 includes a cap body 201, an annular protrusion 202 is disposed on an upper portion of the cap body 201, and the annular protrusion 202 and the cap body 201 form a liquid discharge groove; the size of the outer wall of the filter cup 40 is matched with that of the annular bulge 202, and the filter cup 40 is placed in the liquid discharge tank to take out a cell liquid sample; an air inlet hole 204 for guiding air is arranged on the cover body 201; the air inlet holes 204 are arranged beside the annular bulge 202;
wherein, the bottom of the liquid discharge groove is provided with a sealing ring 203. When the cell liquid collector is used, the filter cup 40 is placed on the sealing ring 203 in the liquid discharge tank, so that the sealing performance can be improved, and the cell liquid is prevented from leaking along the filter cup 40 and the bottom of the liquid discharge tank when a sample is extracted.
With continued reference to FIG. 2, a conduit 205 is disposed at the lower portion of the cover 201, and the conduit 205 is connected to a drainage groove for draining the cell sap sample stored in the vial 30; a filter screen 210 for filtering the cell fluid sample is disposed in the conduit 205. The filter screen 210 may be disposed at the top, middle, or bottom of the conduit 205. In this embodiment, the filter screen 210 is disposed on top of the conduit 205. The filter screen 210 is 100 meshes and 180 meshes, and can well filter impurities and mucus.
In fact, when the cell fluid sample does not need to be drawn, the sealing member 10 is sealed on the bottle cap 20, and the sealing member 10, the bottle cap 20 and the bottle body 30 form a closed environment, so that the cell fluid sample is prevented from being polluted; when the cell sap sampling device is used, the sealing element 10 is opened, the filter cup 40 is placed in the liquid discharge tank, the negative pressure suction machine is connected with the top of the filter cup 40, and the cell sap sample can be extracted by using negative pressure. The specific use state is shown in fig. 7.
With continued reference to fig. 2, a connecting tube 209 extends downward from the bottom of the conduit 205, the connecting tube 209 has an inner diameter and an outer diameter smaller than those of the conduit 205, and the outer portion of the connecting tube 209 forms a step with the conduit 205. In fact, when the bottle cap 20 is mounted on the bottle body 30, the tube structure formed by the conduit 205 and the connecting tube 209 is directly connected to the bottom of the bottle body 30.
To prevent backflow of the cell fluid sample during withdrawal, a water stop ball 208 may be provided in the conduit 205 or a check valve may be provided at the top outlet of the conduit 205. In this embodiment, a water stop ball 208 is disposed in the conduit 205, and the diameter of the water stop ball 208 is larger than that of the connecting pipe 209 and smaller than that of the conduit 205. The water stopping ball 208 is arranged to prevent the cell sap sample from flowing backwards, when the cell sap is extracted under negative pressure, the cell sap flows upwards, and the water stopping ball 208 floats upwards; when the negative pressure is insufficient, the cell sap flows downwards, and the water-stopping ball 208 is blocked at the outlet at the bottom of the conduit 205 under the driving of the cell sap, so that the cell sap is prevented from flowing back.
Specifically, an arc-shaped stop block 206 is arranged in the conduit 205, and the arc-shaped stop block 206 is positioned above the water stop ball 208; a spring 207 is arranged between the arc-shaped stop block 206 and the water-stopping ball 208, and the spring 207 naturally compresses the water-stopping ball 208 to make the water-stopping ball 208 block the outlet at the bottom of the conduit 205. The arrangement can limit the water-stopping ball 208 to prevent the water-stopping ball from flowing out of the top of the conduit 205; on the other hand, the accumulation of cell sap in the catheter 205 can be prevented. In fact, the arc-shaped stoppers 206 can be arranged in a plurality of numbers, and a certain gap exists between the arc-shaped stoppers 206, so that when the negative pressure is large, the water stopping ball 208 compresses the spring 207, the cell fluid sample can continue to flow upwards from the gap, and the stable operation of the extraction work is ensured.
As shown in fig. 3, the sealing member 10 includes a plug 101, a first sealing portion 103 is disposed at the bottom of the plug 101 and adapted to the liquid discharge groove, and a second sealing portion 102 is disposed at one side of the first sealing portion 103 and adapted to the air inlet 204. In practice, the sealing member 10 may also be a sealing membrane covering the top of the cap 20. The sealing film can be a plastic film and is fixed on the bottle cap 20 through hot melt adhesive; when the cell sap negative pressure extraction device is used, the sealing film is torn, the filter cup 40 is placed in the liquid discharge tank, and then the cell sap sample can be extracted under the negative pressure. The reason why the plug body 101 is used for sealing in the present embodiment is that the plug body 101 has better sealing performance to the bottle body 30 than the sealing film, and can prevent the cell fluid sample from flowing from the air inlet hole 204 to the bottle cap 20 when the bottle body 30 is knocked down or turned upside down; meanwhile, the plug body 101 can be recycled, the sealing film needs to be replaced when the plug body 101 is used every time, and cost saving is facilitated by using the plug body 101.
As shown in FIG. 5, a liquid inlet is disposed at the bottom of the filter cup 40, a filter membrane 401 is disposed on the liquid inlet, and a plurality of filter holes for filtering cells are disposed on the filter membrane 401. Furthermore, the aperture of the filtering pores is 8-10 μm. In fact, the diameter of the cell is about 30 μm basically, when the negative pressure is used for extracting, the water in the cell fluid enters the filter cup 40 through the filter membrane 401, the cell sample is adsorbed at the bottom of the filter membrane 401, and the bottom of the filter cup 40 is dipped on the slide glass, so that the cell sample can be extracted into the slide glass.
In this embodiment, the bottle cap 20 and the bottle body 30 are fixed to each other by a screw connection. Set up the fastening nature like this and be convenient for the dismouting good.
In this embodiment, knurls are formed on the outer side of the bottle cap 20 and the outer side of the filter cup 40. The knurling is arranged to facilitate taking of a user and prevent falling off in the taking process.
In a word, the collection assembly for the cell sap sample has a simple integral structure and is convenient to use, the cell sap sample is extracted in a negative pressure extraction mode, non-contact extraction of the cell sap sample is realized, secondary pollution is avoided, the operation steps are simplified, and the working efficiency is improved.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the utility model has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. A collection assembly for a cytosol sample, comprising: the cell liquid sampling device comprises a collecting bottle and a filter cup used for taking a cell liquid sample from the collecting bottle;
the collection bottle comprises a bottle body, a bottle cap and a sealing element; the bottle cap is arranged at the top end of the bottle body, and the sealing element is arranged at the top end of the bottle cap;
the bottle cap comprises a cap body, wherein an annular bulge is arranged at the upper part of the cap body, and the annular bulge and the cap body form a liquid discharge groove; the size of the outer wall of the filter cup is matched with that of the annular bulge, and the filter cup is placed in the liquid discharge tank to take out a cell fluid sample; an air inlet hole for guiding air is formed in the cover body; the air inlet is arranged beside the annular bulge;
a guide pipe is arranged at the lower part of the cover body and is communicated with the liquid discharge groove so as to discharge the cell sap sample stored in the bottle body; and a filter screen for filtering the cell sap sample is arranged in the conduit.
2. The collection assembly for cellular fluid samples according to claim 1, wherein the bottom of the conduit is provided with a connecting pipe extending downwards, the inner diameter and the outer diameter of the connecting pipe are both smaller than those of the conduit, and the outer part of the connecting pipe forms a step with the conduit.
3. The collection assembly of a cytosol sample according to claim 2 wherein said conduit has a water stop ball disposed therein, said water stop ball having a diameter greater than a diameter of said connecting tube and less than a diameter of said conduit.
4. The collection assembly of a cellular fluid sample according to claim 3, wherein an arc-shaped stopper is arranged in the conduit, and the arc-shaped stopper is positioned above the water stop ball; a spring is arranged between the arc-shaped stop block and the water stopping ball, and the water stopping ball is compressed by the spring in a natural state so that the outlet at the bottom of the conduit is blocked by the water stopping ball.
5. The cytosol sample collection assembly as claimed in claim 1, wherein said sealing member is a sealing membrane covering the top of said cap.
6. The collection assembly of cellular fluid samples according to claim 1, wherein the sealing member comprises a plug body, the bottom of the plug body is provided with a first sealing part matched with the drainage groove, and one side of the first sealing part is provided with a second sealing part matched with the air inlet hole.
7. The collection assembly for a cellular fluid sample according to claim 1, wherein the bottom of the drainage groove is provided with a sealing ring.
8. The collection assembly for a cellular fluid sample according to claim 1, wherein the bottom of the filter cup is provided with a liquid inlet, the liquid inlet is provided with a filter membrane, and the filter membrane is provided with a plurality of filter holes for filtering cells.
9. The cytosol sample collection assembly as claimed in claim 1, wherein said filter screen is disposed at the top of said conduit.
10. The cytosol sample collection assembly as claimed in claim 1, wherein said cap outer side and said filter cup outer side are knurled.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122801811.XU CN216349779U (en) | 2021-11-16 | 2021-11-16 | Collecting assembly for cell sap samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122801811.XU CN216349779U (en) | 2021-11-16 | 2021-11-16 | Collecting assembly for cell sap samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216349779U true CN216349779U (en) | 2022-04-19 |
Family
ID=81149648
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122801811.XU Active CN216349779U (en) | 2021-11-16 | 2021-11-16 | Collecting assembly for cell sap samples |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216349779U (en) |
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2021
- 2021-11-16 CN CN202122801811.XU patent/CN216349779U/en active Active
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