CN220926808U - Cell culture dish convenient to sample - Google Patents

Abstract

The utility model relates to the technical field of cell culture, in particular to a cell culture dish convenient for sampling, which comprises a culture dish body, wherein a sealing cover is covered on the surface of the culture dish body, a liquid inlet pipe is fixed on one side of the culture dish body, a liquid discharge pipe is fixed on one side of the bottom position of the culture dish body, sealing plugs are filled at one ends of the liquid discharge pipe and the liquid inlet pipe, a stabilizing mechanism is arranged at the bottom position of the culture dish body, the stabilizing mechanism consists of a placing seat and a stabilizing assembly, a sampling mechanism is arranged at the top position of the sealing cover body, the sampling mechanism consists of a sampling part and a plug, a gas filtering mechanism is arranged at the top position of the sealing cover body, and the gas filtering mechanism consists of a gas filtering member and a blocking cover. The utility model not only improves the convenience degree of the cell culture dish during sampling, avoids the phenomenon that the culture solution is polluted when the cell culture dish is used, but also improves the stability of the cell culture dish during use.

Description

Cell culture dish convenient to sample

Technical Field

The utility model relates to the technical field of cell culture, in particular to a cell culture dish convenient for sampling.

Background

The cell culture dish is a laboratory vessel for microorganism or cell culture, and consists of a flat disc-shaped bottom and a cover, wherein the cell culture dish is basically divided into two types, mainly plastic and glass, the former can be used for plant material, microorganism culture and animal cell adherent culture, and the latter is mostly made of polyethylene material, is suitable for laboratory inoculation, streaking and bacteria separation operation, and can be used for plant material culture.

Because the culture medium in the cell culture dish can be exposed to the external environment in a large area when the dish cover is opened for sampling, the pollution of the culture medium is easy to cause, and the sampling is complicated when the cover is not opened, so that the convenience of sampling the cell culture dish is affected; when the cell culture dish is used, part of cells need to be sealed and the other part needs to be contacted with air, and when the cell culture dish is used, the introduced air is not easy to filter, so that the phenomenon that the culture solution is polluted when the cell culture dish is introduced with air is easy to cause; cell culture dishes are generally smaller and lighter, and have lower stability when placed, thereby affecting the stability of the cell culture dish when in use.

Disclosure of utility model

The utility model aims to provide a cell culture dish convenient for sampling, which solves the problems of low convenience degree, easy pollution of culture solution and low stability when the cell culture dish is used for sampling in the prior art.

In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a cell culture dish convenient to sample, includes the culture dish body, the surface lid of culture dish body is equipped with sealed lid, one side of culture dish body is fixed with the feed liquor pipe, one side of culture dish body bottom position department is fixed with the fluid-discharge tube, the sealing plug has all been filled to one end of fluid-discharge tube and feed liquor pipe, the bottom position department of culture dish body is provided with stabilizing mean, stabilizing mean comprises placing seat and stabilizing subassembly, the top position department of sealed lid is provided with sampling mechanism, sampling mechanism comprises sampling component and end cap, the top position department of sealed lid is provided with gas filtration mechanism, gas filtration mechanism comprises gas filtration component and end cap.

Preferably, a placing seat is sleeved at the bottom of the culture dish body, and a stabilizing component is arranged on the surface of the placing seat.

Preferably, the inside of stabilizing assembly is including fixture block, draw-in groove and sucking disc, the fixture block is all installed to the bottom position department of the culture dish body, the draw-in groove has been seted up along circumference to the inner wall of placing the seat, the draw-in groove cooperates with the mutual joint of fixture block, the bottom position department of placing the seat is fixed with the sucking disc along circumference.

Preferably, a sampling component is arranged at the top position of the sealing cover body, and a plug is plugged at one end of the sampling component.

Preferably, the inside of sampling part is provided with sampling tube, fixed section of thick bamboo, extension spring and seal cover, the top position department of sealed lid is fixed with fixed section of thick bamboo, the inside of fixed section of thick bamboo is provided with the sampling tube.

Preferably, a sealing sleeve is adhered to the inner wall of the fixed cylinder, the sealing sleeve is tightly attached to the surface of the sampling tube, an extension spring is sleeved on the surface of the sampling tube, and the surface of the extension spring is fixedly connected with the surface of the fixed cylinder.

Preferably, a gas filtering member is fixed at the top position of the liquid discharge pipe, and a blocking cover is arranged at one end cover of the gas filtering member.

Preferably, the inside of gas filtration component includes dog, inlet hood and filter layer, the top position department of sealed lid is fixed with the inlet hood, the mutual screw-thread fit of inlet hood and fender lid.

Preferably, the two sides of the inner wall of the air inlet cover are both fixed with the stop blocks, the filter layer is arranged in the air inlet cover, and the filter layer is matched with the stop blocks in a clamping manner.

Compared with the prior art, the utility model has the beneficial effects that: the cell culture dish convenient for sampling not only improves the convenience degree of the cell culture dish during sampling, avoids the phenomenon that the culture solution is polluted when the cell culture dish is used, but also improves the stability of the cell culture dish when the cell culture dish is used;

1. Through being provided with the sampling mechanism, pull out the end cap, insert the inside of sampling tube with sampling needle tubing one end again, can push down the sampling tube and make the sampling tube remove to wait to take a sample afterwards, at this moment, can make outside dust be difficult for getting into the culture dish internal portion when the sampling tube removes under the effect of seal cover, then through drawing out the sampling needle tubing, take out the sample, pack the end cap to one end of sampling tube again, and reset the sampling tube under the effect of extension spring, in order to realize the function that the cell culture dish is convenient for take a sample, thereby improved the convenience degree when the cell culture dish takes a sample;

2. through being provided with gas filtering mechanism, make the fender lid fix the one end at the air inlet cover under the screw-thread fit of fender lid and air inlet cover, can seal the air inlet cover under the effect of fender lid, seal the cell culture dish, if need to be to the inside air of leading-in of culture dish body, rotatable fender lid is taken off, at this moment, the air filters under the effect of filter layer, the inside of the gas leading-in culture dish body of filtration after the air through the air inlet cover, simultaneously, can stimulate the filter layer, split filter layer and dog, thereby take out the filter layer and change, with the filter function of realization cell culture dish to the entering air, thereby the phenomenon that the culture solution takes place to pollute when having avoided the cell culture dish to use;

3. Through being provided with stabilizing mean, put the culture dish body to the inside of placing the seat, make the inside of fixture block card to draw-in groove, it is fixed with the culture dish body with placing the seat under the card action of fixture block and draw-in groove, can put the culture dish body to relevant position department afterwards, can adsorb the seat of placing under the effect of sucking disc and fix in the department of placing, improve the stability of the culture dish body to realize the auxiliary fixation function of cell culture dish, thereby improved the stability when cell culture dish used.

Drawings

FIG. 1 is a schematic view of a three-dimensional appearance structure of the present utility model;

FIG. 2 is a schematic view of a front cross-sectional structure of the present utility model;

FIG. 3 is an enlarged schematic view of the gas filtering mechanism of the present utility model;

FIG. 4 is an enlarged schematic view of the sampling mechanism according to the present utility model.

In the figure: 1. a culture dish body; 101. sealing cover; 102. a liquid inlet pipe; 103. a liquid discharge pipe; 2. a stabilizing mechanism; 201. a placement seat; 202. a stabilizing assembly; 2021. a clamping block; 2022. a clamping groove; 2023. a suction cup; 3. a sampling mechanism; 301. a sampling part; 3011. a sampling tube; 3012. a fixed cylinder; 3013. an extension spring; 3014. sealing sleeve; 302. a plug; 4. a gas filtering mechanism; 401. a gas filtering member; 4011. a stop block; 4012. an air inlet cover; 4013. a filter layer; 402. and a blocking cover.

Detailed Description

The technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are only some embodiments of the present utility model, not all embodiments, and furthermore, the terms "first", "second", "third", "up, down, left, right", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Meanwhile, in the description of the present utility model, unless explicitly stated and defined otherwise, the terms "connected", "connected" and "connected" should be interpreted broadly, and for example, may be fixedly connected, detachably connected, or integrally connected; the mechanical connection and the electrical connection can be adopted; all other embodiments, which may be directly or indirectly through intermediaries, which may be obtained by a person of ordinary skill in the art without inventive effort based on the embodiments of the present utility model are within the scope of the present utility model.

The structure of the cell culture dish convenient for sampling provided by the utility model is shown in fig. 1 and 2, and comprises a culture dish body 1, wherein a sealing cover body 101 is covered on the surface of the culture dish body 1, a liquid inlet pipe 102 is fixed on one side of the culture dish body 1, a liquid discharge pipe 103 is fixed on one side of the bottom position of the culture dish body 1, and sealing plugs are filled at one ends of the liquid discharge pipe 103 and the liquid inlet pipe 102.

When the culture dish is used, the sealing cover body 101 is covered on the surface of the culture dish body 1, the culture dish body 1 is sealed, then culture solution is injected into the culture dish body 1 through the liquid inlet pipe 102, the culture solution can be discharged through the liquid outlet pipe 103, and the liquid inlet pipe 102 and the liquid outlet pipe 103 can be blocked under the action of blocking the inside of the liquid inlet pipe 102 and the liquid outlet pipe 103.

Further, as shown in fig. 2, a stabilizing mechanism 2 is arranged at the bottom position of the culture dish body 1, the stabilizing mechanism 2 is composed of a placing seat 201 and a stabilizing component 202, the placing seat 201 is sleeved at the bottom position of the culture dish body 1, the stabilizing component 202 is arranged on the surface of the placing seat 201, a clamping block 2021, a clamping groove 2022 and a sucking disc 2023 are arranged inside the stabilizing component 202, the clamping block 2021 is arranged at the bottom position of the culture dish body 1, the clamping groove 2022 is formed in the inner wall of the placing seat 201 along the circumferential direction, the clamping groove 2022 is matched with the clamping block 2021 in a mutually clamping manner, and the sucking disc 2023 is fixed at the bottom position of the placing seat 201 along the circumferential direction.

When the culture dish body 1 is used, the culture dish body 1 is placed in the placing seat 201, the clamping block 2021 is clamped in the clamping groove 2022, the culture dish body 1 is fixed with the placing seat 201 under the clamping action of the clamping block 2021 and the clamping groove 2022, then the culture dish body 1 can be placed at the corresponding position, the placing seat 201 can be adsorbed and fixed at the placing position under the action of the sucking disc 2023, and the stability of the culture dish body 1 is improved, so that the auxiliary fixing function of the cell culture dish is realized.

Further, as shown in fig. 4, a sampling mechanism 3 is disposed at the top position of the sealing cover body 101, the sampling mechanism 3 is composed of a sampling component 301 and a plug 302, the sampling component 301 is disposed at the top position of the sealing cover body 101, the plug 302 is plugged at one end of the sampling component 301, a sampling tube 3011, a fixing tube 3012, an expansion spring 3013 and a sealing sleeve 3014 are disposed inside the sampling component 301, the fixing tube 3012 is fixed at the top position of the sealing cover body 101, the sampling tube 3011 is disposed inside the fixing tube 3012, the sealing sleeve 3014 is adhered to the inner wall of the fixing tube 3012, the sealing sleeve 3014 is tightly adhered to the surface of the sampling tube 3011, the expansion spring 3013 is sleeved on the surface of the sampling tube 3011, and the surface of the expansion spring 3013 is fixedly connected with the surface of the fixing tube 3012.

During the use, pull out the end cap 302, insert the inside of sampling tube 3011 with sampling needle tubing one end again, can press sampling tube 3011 afterwards and make sampling tube 3011 remove to waiting the sample department, at this moment, can make outside dust be difficult for getting into inside the culture dish body 1 when sampling tube 3011 removes under the effect of seal cover 3014, later take out the sample through drawing the sampling needle tubing, pack the end cap 302 to the one end of sampling tube 3011 again, and make sampling tube 3011 reset under the effect of extension spring 3013, in order to realize the function of the convenient sample of cell culture dish.

Further, as shown in fig. 3, a gas filtering mechanism 4 is disposed at the top position of the sealing cover body 101, the gas filtering mechanism 4 is composed of a gas filtering component 401 and a blocking cover 402, the gas filtering component 401 is fixed at the top position of the liquid discharging pipe 103, a blocking cover 402 is disposed at one end cover of the gas filtering component 401, a stop block 4011, a gas inlet hood 4012 and a filter layer 4013 are included in the gas filtering component 401, the gas inlet hood 4012 is fixed at the top position of the sealing cover body 101, the gas inlet hood 4012 is in threaded fit with the blocking cover 402, the stop block 4011 is fixed at two sides of the inner wall of the gas inlet hood 4012, the filter layer 4013 is in clamped fit with the stop block 4011.

During the use, make keep off lid 402 fixed in the one end of air inlet cover 4012 under the screw-thread fit of keeping off lid 402 and air inlet cover 4012, can seal air inlet cover 4012 under the effect of keeping off lid 402, seal the cell culture dish, if need to be to the inside air of culture dish body 1, rotatable keep off lid 402 takes off the cover 402, at this moment, the air filters under the effect of filter layer 4013, the inside of the gas after the filtration is leading into culture dish body 1 through air inlet cover 4012, simultaneously, can stimulate filter layer 4013, split filter layer 4013 and dog 4011, thereby take out filter layer 4013 and change, in order to realize the filter function of cell culture dish to the entering air.

Working principle: when the culture dish body 1 is used, firstly, the culture dish body 1 can be placed in the placement seat 201, the clamping block 2021 is clamped in the clamping groove 2022, the culture dish body 1 is fixed with the placement seat 201 under the clamping action of the clamping block 2021 and the clamping groove 2022, then the culture dish body 1 can be placed at the corresponding position, the placement seat 201 can be adsorbed and fixed at the placement position under the action of the sucking disc 2023, the stability of the culture dish body 1 is improved, the auxiliary fixing function of the cell culture dish is realized, and the stability of the cell culture dish in use is improved.

The sealing cover body 101 is covered on the surface of the culture dish body 1 to seal the culture dish body 1, then the culture solution is injected into the culture dish body 1 through the liquid inlet pipe 102, the culture solution can be discharged through the liquid outlet pipe 103, and the liquid inlet pipe 102 and the liquid outlet pipe 103 can be blocked under the action of blocking the inside of the liquid inlet pipe 102 and the liquid outlet pipe 103.

At this time, the blocking cover 402 is fixed at one end of the air inlet cover 4012 under the threaded fit of the blocking cover 402 and the air inlet cover 4012, the air inlet cover 4012 can be blocked under the action of the blocking cover 402 to seal the cell culture dish, if air is required to be introduced into the culture dish body 1, the blocking cover 402 can be rotated to remove the blocking cover 402, at this time, air is filtered under the action of the filtering layer 4013, the filtered air is introduced into the culture dish body 1 through the air inlet cover 4012, meanwhile, the filtering layer 4013 can be pulled to split the filtering layer 4013 from the stop block 4011, so that the filtering layer 4013 is taken out for replacement, the filtering function of the cell culture dish on the inlet air is realized, and the phenomenon that the culture solution is polluted when the cell culture dish is used is avoided.

If need sample time, the plug 302 can be pulled down, insert the inside of sampling tube 3011 with sampling needle tubing one end again, can press sampling tube 3011 afterwards and make sampling tube 3011 remove to waiting the sample department, at this moment, can make outside dust be difficult for getting into inside the culture dish body 1 when sampling tube 3011 removes under the effect of seal cover 3014, later take out the sample through drawing out the sampling needle tubing, pack the plug 302 to the one end of sampling tube 3011 again, and make sampling tube 3011 reset under the effect of extension spring 3013, so as to realize the function that the cell culture dish is convenient for sample, thereby the convenience degree when having improved the cell culture dish sample, finally accomplish the use work of cell culture dish.

It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present utility model may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (9)

1. Cell culture dish convenient to sample, including culture dish body (1), its characterized in that: the surface lid of culture dish body (1) is equipped with sealed lid (101), one side of culture dish body (1) is fixed with feed liquor pipe (102), one side of culture dish body (1) bottom position department is fixed with fluid-discharge tube (103), fluid-discharge tube (103) all have filled the sealing plug with the one end of feed liquor pipe (102), the bottom position department of culture dish body (1) is provided with stabilizing mean (2), stabilizing mean (2) are by placing seat (201) and stabilizing subassembly (202) and are constituteed, the top position department of sealed lid (101) is provided with sampling mechanism (3), sampling mechanism (3) are constituteed by sampling part (301) and end cap (302), the top position department of sealed lid (101) is provided with gas filtration mechanism (4), gas filtration mechanism (4) are constituteed by gas filtration component (401) and end cap (402).

2. A cell culture dish for facilitating sampling according to claim 1, wherein: the bottom position of the culture dish body (1) is sleeved with a placing seat (201), and a stabilizing component (202) is arranged on the surface of the placing seat (201).

3. A cell culture dish for facilitating sampling according to claim 2, wherein: the inside of stable subassembly (202) is including fixture block (2021), draw-in groove (2022) and sucking disc (2023), fixture block (2021) are all installed to the bottom position department of culture dish body (1), draw-in groove (2022) have been seted up along circumference to the inner wall of placing seat (201), draw-in groove (2022) and the mutual joint cooperation of fixture block (2021), the bottom position department of placing seat (201) is fixed with sucking disc (2023) along circumference.

4. A cell culture dish for facilitating sampling according to claim 1, wherein: a sampling component (301) is arranged at the top position of the sealing cover body (101), and a plug (302) is plugged at one end of the sampling component (301).

5. The cell culture dish for facilitating sampling according to claim 4, wherein: the inside of sampling part (301) is provided with sampling tube (3011), fixed section of thick bamboo (3012), extension spring (3013) and seal cover (3014), the top position department of sealed lid (101) is fixed with fixed section of thick bamboo (3012), the inside of fixed section of thick bamboo (3012) is provided with sampling tube (3011).

6. The cell culture dish for facilitating sampling according to claim 5, wherein: the inner wall of the fixed barrel (3012) is adhered with a sealing sleeve (3014), the sealing sleeve (3014) is tightly adhered to the surface of the sampling tube (3011), an extension spring (3013) is sleeved on the surface of the sampling tube (3011), and the surface of the extension spring (3013) is fixedly connected with the surface of the fixed barrel (3012).

7. A cell culture dish for facilitating sampling according to claim 1, wherein: a gas filtering component (401) is fixed at the top position of the liquid discharge pipe (103), and a blocking cover (402) is arranged on one end cover of the gas filtering component (401).

8. The cell culture dish for facilitating sampling according to claim 7, wherein: the inside of gas filtration component (401) is including dog (4011), inlet cowl (4012) and filter layer (4013), the top position department of sealed lid (101) is fixed with inlet cowl (4012), the mutual screw thread fit of inlet cowl (4012) and fender lid (402).

9. The cell culture dish for facilitating sampling according to claim 8, wherein: both sides of the inner wall of the air inlet cover (4012) are fixed with stop blocks (4011), a filter layer (4013) is arranged in the air inlet cover (4012), and the filter layer (4013) is matched with the stop blocks (4011) in a mutually clamping mode.