CN217436618U - Liquid-based cell preservation liquid bottle assembly convenient for cell collection - Google Patents
Liquid-based cell preservation liquid bottle assembly convenient for cell collection Download PDFInfo
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- CN217436618U CN217436618U CN202123402573.1U CN202123402573U CN217436618U CN 217436618 U CN217436618 U CN 217436618U CN 202123402573 U CN202123402573 U CN 202123402573U CN 217436618 U CN217436618 U CN 217436618U
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- filtering membrane
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Abstract
The utility model provides a liquid-based cell preservation liquid bottle subassembly convenient to gather cell, include: a preservation bottle for storing cell preservation solution and a filtering membrane cylinder for collecting cells; the preservation bottle comprises a bottle body and a bottle cap, and the bottle cap is arranged at the top of the bottle body; the bottle cap is characterized in that an annular liquid storage tank is arranged at the top of the bottle cap, a piston assembly is arranged in the liquid storage tank, the piston assembly comprises a piston pad, and a liquid outlet hole for leading out preservation liquid in the bottle body is formed in the piston pad; an annular bulge is arranged on the piston pad, the size of the filtering membrane cylinder is matched with that of the annular bulge, and the filtering membrane cylinder presses the annular bulge so that the piston pad moves downwards in the liquid storage tank; a plurality of gaps are arranged on the annular bulge. The utility model discloses a liquid-based cell preservation subassembly overall structure is simple, and convenient to use can realize having avoided secondary pollution to the contactless extraction of cell, has simplified the operating procedure simultaneously, has improved work efficiency.
Description
Technical Field
The utility model relates to a film type liquid-based cell film-making machine cell sample liquid gathers film-making technical field, particularly, relates to a liquid-based cell preservation liquid bottle subassembly convenient to gather cell.
Background
The core of the membrane type liquid-based cell pelleter is a cell filtration transfer adsorption technology, and according to the principle of liquid-based cytology, the cell is adsorbed by adopting a physical suction and filter membrane technology and then transferred onto a glass slide to form a uniform cell thin layer on the glass slide, so that further dyeing and diagnosis are facilitated.
In the prior art, the bottle cap of the liquid storage bottle used by the membrane type liquid-based cell pelleter needs to be unscrewed manually when the liquid storage bottle is used, the human cell sample liquid is taken out of the liquid storage bottle through a dropper or other tools and put into the pelleter, the bottle cap is opened in the process, the secondary pollution is increased, the working steps of experimenters are increased, and the efficiency is reduced.
In view of this, the present invention is especially provided.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a liquid base cell preservation liquid bottle subassembly convenient to gather cell, this subassembly overall structure is simple, and is cooperated with the preservation bottle through setting up a filtration membrane section of thick bamboo, can directly gather the cell in the cell preservation liquid, need not to open the bottle lid to avoid secondary pollution, simplified operating procedure simultaneously, improved work efficiency.
In order to realize the above purpose of the utility model, the following technical scheme is adopted:
the utility model provides a liquid-based cell preservation liquid bottle subassembly convenient to gather cell, include: a preservation bottle for storing cell preservation solution and a filtering membrane cylinder for collecting cells;
the preservation bottle comprises a bottle body and a bottle cap, and the bottle cap is arranged at the top of the bottle body;
an annular liquid storage tank is arranged at the top of the bottle cap, a piston assembly is arranged in the liquid storage tank,
the piston assembly comprises a piston pad, and a liquid outlet hole for guiding out the preservation liquid in the bottle body is formed in the piston pad;
an annular bulge is arranged on the piston pad, the size of the filtering membrane cylinder is matched with that of the annular bulge, and the filtering membrane cylinder presses the annular bulge so that the piston pad moves downwards in the liquid storage tank; a plurality of gaps are arranged on the annular bulge.
In the prior art, the bottle cap of the liquid storage bottle used by the membrane type liquid-based cell pelleter needs to be unscrewed manually when the liquid storage bottle is used, the human cell sample liquid is taken out of the liquid storage bottle through a dropper or other tools and put into the pelleter, the bottle cap is opened in the process, the secondary pollution is increased, the working steps of experimenters are increased, and the efficiency is reduced.
In order to solve the technical problem, the utility model provides a liquid-based cell preservation liquid bottle assembly convenient for collecting cells, which is matched with a filtering membrane cylinder by arranging a preservation bottle without opening a bottle cap, realizes the non-contact extraction of a cell liquid sample, avoids secondary pollution, simplifies the operation steps and improves the working efficiency; through set up the annular protruding on the piston pad, can press down the protruding piston pad downstream that makes of annular through filtration membrane cylinder to make cell preservation liquid upwards carry from the bottle, and flow into in the space between filtration membrane cylinder and reservoir through the breach on the annular is protruding, like this when the negative pressure extraction, can directly extract the cell preservation liquid in the reservoir, need not to extract the cell preservation liquid in the bottle, can shorten the acquisition time, improve collection efficiency.
Preferably, the bottom of the liquid storage tank is provided with a limiting ring, and the inner diameter of the limiting ring is smaller than the diameter of the piston pad. Set up the spacing ring and can play limiting displacement to the piston pad, prevent to press the in-process piston pad and fall into in the bottle.
Preferably, a liquid guide pipe is arranged at the lower part of the liquid outlet hole, and when the piston pad is pressed to be in contact with the limiting ring, the liquid guide pipe is communicated with liquid at the bottom of the bottle body so as to discharge the liquid at the bottom of the bottle body into the liquid storage tank through positive pressure.
Preferably, a filter screen for filtering the cell preservation solution is arranged on the liquid outlet hole.
Preferably, a sealing plug is arranged at the top of the liquid storage tank, the sealing plug comprises a plug body, and a sealing part matched with the annular bulge is arranged at the bottom of the plug body. The sealing plug is arranged to play a role in sealing, so that the cell preservation solution in the bottle body is prevented from being polluted.
Preferably, a liquid inlet is formed in the bottom of the filtering membrane cylinder, a filtering membrane and a buffering sponge cushion are arranged on the liquid inlet, and the buffering sponge cushion is arranged below the filtering membrane; the filtering membrane is provided with a plurality of filtering holes for filtering cells. Furthermore, the number of the filtering holes is tens of thousands, and the aperture of the filtering hole is 8-10 μm. In fact, the diameter of the cells is about 30 μm basically, when negative pressure extraction is carried out, water in the cell preservation solution enters the filtering membrane cylinder through the filtering membrane, the cell sample is adsorbed at the bottom of the filtering membrane, and the bottom of the filtering membrane is dipped on the glass slide, so that the cell sample can be extracted into the glass slide.
Preferably, knurls are arranged on the outer side of the bottle cap and the outer side of the filtering membrane cylinder.
Preferably, the outer wall of the piston pad is provided with a sealing ring. Set up the sealing washer and can improve sealed effect, guarantee the stability of cell preservation liquid extraction.
Compared with the prior art, the beneficial effects of the utility model reside in that:
(1) the liquid-based cell preservation liquid bottle component convenient for cell collection is matched with the filtering membrane cylinder by arranging the preservation bottle, the bottle cap does not need to be opened, the cell liquid sample is extracted without contact, the secondary pollution is avoided, the operation steps are simplified, and the working efficiency is improved;
(2) the annular bulge is arranged on the piston pad, so that the piston pad can move downwards by pressing the annular bulge through the filtering membrane cylinder, cell preservation liquid is conveyed upwards from the bottle body and flows into a gap between the filtering membrane cylinder and the liquid storage tank through the notch on the annular bulge, the collection time is shortened, and the collection efficiency is improved;
(3) through setting up the sealing plug, can prevent that the cell preservation liquid in the bottle body from receiving the pollution.
Drawings
Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the invention. Also, like reference numerals are used to refer to like parts throughout the drawings. In the drawings:
fig. 1 is a schematic view of an external structure of a storage bottle provided by an embodiment of the present invention;
fig. 2 is a schematic view of the internal structure of the storage bottle provided by the embodiment of the present invention;
fig. 3 is a schematic view of a use state of the preservation bottle provided by the embodiment of the present invention;
fig. 4 is a schematic structural diagram of a filtering membrane cartridge according to an embodiment of the present invention.
Wherein:
1-bottle body; 2-a catheter;
3-bottle cap; 4-a limiting ring;
5-a piston pad; 6-annular protrusion;
7-notch; 8-a liquid storage tank;
9-a filtration membrane cartridge; 10-a filter screen;
11-a sealing ring; 12-a sealing plug;
13-a filtration membrane; 14-buffer sponge cushion.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings and detailed description, but those skilled in the art will understand that the following described embodiments are some, not all, of the embodiments of the present invention, and are only used for illustrating the present invention, and should not be construed as limiting the scope of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In order to explain the technical solution of the present invention more clearly, the following description is made in the form of specific embodiments.
Examples
Referring to fig. 1-4, the present embodiment provides a liquid-based cell preservation solution bottle assembly for facilitating cell collection, comprising: a preservation bottle for storing cell preservation solution and a filtering membrane cylinder 9 for collecting cells;
as shown in fig. 2, the preservation bottle comprises a bottle body 1 and a bottle cap 3, wherein the bottle cap 3 is arranged at the top of the bottle body 1; an annular liquid storage tank 8 is arranged at the top of the bottle cap 3, a piston assembly is arranged in the liquid storage tank 8,
wherein, the piston assembly comprises a piston pad 5, the outer wall of the piston pad 5 is provided with a sealing ring 11, and the piston pad 5 is provided with a liquid outlet hole for guiding out the preservation liquid in the bottle body 1; the lower part of the liquid outlet hole is provided with a liquid guide pipe 2, when the piston pad 5 is pressed to be in contact with the limiting ring 4, the liquid guide pipe 2 is communicated with the liquid at the bottom of the bottle body 1 so as to discharge the liquid at the bottom of the bottle body into the liquid storage tank through positive pressure.
Specifically, a filter screen 10 for filtering the cell preservation solution is arranged on the liquid outlet hole.
Continuing to refer to fig. 2, the piston pad 5 is provided with an annular protrusion 6, the size of the filtering membrane cylinder 9 is matched with that of the annular protrusion 6, and the filtering membrane cylinder 9 presses the annular protrusion 6 to enable the piston pad 5 to move downwards in the liquid storage tank 8; the annular protrusion 6 is provided with a plurality of notches 7.
In order to prevent the piston pad 5 from falling into the bottle body 1 during the pressing process, the bottom of the liquid storage tank 8 is provided with the limiting ring 4, and the inner diameter of the limiting ring 4 is smaller than the diameter of the piston pad 5.
With continued reference to fig. 2, a sealing plug 12 is disposed at the top of the reservoir 8, the sealing plug 12 includes a plug body, and a sealing portion fitted with the annular protrusion 6 is disposed at the bottom of the plug body. The sealing plug 12 is provided to seal the cell preservation solution in the bottle body 1 and prevent the cell preservation solution from being contaminated. In practice, the cap 3 may also be sealed by a sealing film, in particular, covering the top of the reservoir 8.
As shown in fig. 4, a liquid inlet is formed in the bottom of the filtering membrane cylinder 9, a filtering membrane 13 and a buffering spongy cushion 14 are arranged on the liquid inlet, and the buffering spongy cushion 14 is arranged below the filtering membrane 13; the filtration membrane 13 is provided with a plurality of filtration pores for filtering cells. The number of the filtering holes is tens of thousands, and the aperture of the filtering hole is 8-10 μm. Actually, the diameter of the cells is about 30 μm basically, and when the cells are extracted under negative pressure, water in the cell preservation solution enters the filtering membrane cylinder 9 through the filtering membrane, the cell sample is adsorbed at the bottom of the filtering membrane 13, and the bottom of the filtering membrane 13 is dipped on the glass slide, so that the cell sample can be extracted into the glass slide.
In this embodiment, for the convenience takes the storage bottle, the 3 outsides of bottle lid are provided with the annular knurl.
The working principle of the saving component of the embodiment is as follows:
firstly, the sealing plug 12 is taken down, the piston assembly is pressed through the filtering membrane cylinder 9, the piston pad 5 moves downwards, and the preservation solution in the bottle body 1 flows into a gap between the filtering membrane cylinder 9 and the liquid storage tank 8 through the liquid outlet hole and the notch 7 on the annular bulge 6 along the liquid guide pipe 2; then, the cell preservation solution is pumped into the filtering membrane cylinder 9 through negative pressure, and cells in the cell preservation solution are adsorbed at the bottom of the filtering membrane 13; finally, the bottom of the filter membrane 13 is dipped on the slide, and the cell sample is extracted into the slide. The specific use state is shown in fig. 3.
In a word, the utility model discloses a liquid-based cell preservation subassembly overall structure is simple, and convenient to use can realize having avoided secondary pollution to the contactless extraction of cell, has simplified operating procedure simultaneously, has improved work efficiency.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention.
Claims (8)
1. A liquid-based cell preservation liquid bottle assembly facilitating collection of cells, comprising: a preservation bottle for storing cell preservation solution and a filtering membrane cylinder for collecting cells;
the preservation bottle comprises a bottle body and a bottle cap, and the bottle cap is arranged at the top of the bottle body;
the top of the bottle cap is provided with an annular liquid storage tank, a piston assembly is arranged in the liquid storage tank,
the piston assembly comprises a piston pad, and a liquid outlet hole for guiding out the preservation liquid in the bottle body is formed in the piston pad;
an annular bulge is arranged on the piston pad, the size of the filtering membrane cylinder is matched with that of the annular bulge, and the filtering membrane cylinder presses the annular bulge so that the piston pad moves downwards in the liquid storage tank; a plurality of gaps are arranged on the annular bulge.
2. The liquid-based cell preservation liquid bottle assembly according to claim 1, wherein the bottom of the liquid storage tank is provided with a limiting ring, and the inner diameter of the limiting ring is smaller than the diameter of the piston pad.
3. The assembly as claimed in claim 2, wherein a liquid guiding tube is disposed at a lower portion of the liquid outlet hole, and when the piston pad is pressed into contact with the limiting ring, the liquid guiding tube is in liquid communication with the bottom of the bottle body to discharge the liquid at the bottom of the bottle body into the liquid storage tank by positive pressure.
4. The liquid-based cell preservation liquid bottle assembly according to claim 1, wherein a filter screen for filtering the cell preservation liquid is disposed on the liquid outlet hole.
5. The liquid-based cell preservation liquid bottle assembly as claimed in claim 1, wherein a sealing plug is arranged on the top of the liquid storage tank, the sealing plug comprises a plug body, and a sealing part matched with the annular bulge is arranged on the bottom of the plug body.
6. The liquid-based cell preservation liquid bottle assembly according to claim 1, wherein a liquid inlet is arranged at the bottom of the filtering membrane cylinder, a filtering membrane and a buffering sponge pad are arranged on the liquid inlet, and the buffering sponge pad is arranged below the filtering membrane; the filtering membrane is provided with a plurality of filtering holes for filtering cells.
7. The liquid-based cell preservation solution vial assembly of claim 1, wherein the outside of the vial cap is provided with knurling.
8. The liquid-based cell preservation solution vial assembly of claim 1, wherein the outer wall of the piston pad is provided with a sealing ring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123402573.1U CN217436618U (en) | 2021-12-31 | 2021-12-31 | Liquid-based cell preservation liquid bottle assembly convenient for cell collection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123402573.1U CN217436618U (en) | 2021-12-31 | 2021-12-31 | Liquid-based cell preservation liquid bottle assembly convenient for cell collection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217436618U true CN217436618U (en) | 2022-09-16 |
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ID=83211579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123402573.1U Active CN217436618U (en) | 2021-12-31 | 2021-12-31 | Liquid-based cell preservation liquid bottle assembly convenient for cell collection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217436618U (en) |
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2021
- 2021-12-31 CN CN202123402573.1U patent/CN217436618U/en active Active
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