CN216309887U - Chromatographic separation device for gradient elution - Google Patents
Chromatographic separation device for gradient elution Download PDFInfo
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- CN216309887U CN216309887U CN202121874702.4U CN202121874702U CN216309887U CN 216309887 U CN216309887 U CN 216309887U CN 202121874702 U CN202121874702 U CN 202121874702U CN 216309887 U CN216309887 U CN 216309887U
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Abstract
The utility model discloses a chromatographic separation device for gradient elution, which comprises a rack and an installation rack, wherein a raw material metering pump, an eluent A metering pump, an eluent B metering pump, a motor and an eluent C metering pump are sequentially arranged at the upper part of the rack from left to right, a constant-temperature water tank is arranged at the lower part of the rack, a material container, an eluent A container, an eluent B container and an eluent C container are sequentially arranged in the constant-temperature water tank from left to right, a first chromatographic separation column, a second chromatographic separation column, a third chromatographic separation column and a fourth chromatographic separation column are sequentially arranged on the installation rack from left to right, and a circulating communication mechanism is arranged among the first chromatographic separation column, the second chromatographic separation column, the third chromatographic separation column and the fourth chromatographic separation column. The chromatographic separation device for gradient elution has the advantages of reasonable structural design, low cost and simple and convenient operation, and is suitable for separating materials with complicated components and difficult separation.
Description
Technical Field
The utility model relates to the technical field of chromatographic separation, in particular to a chromatographic separation device for gradient elution.
Background
The chromatographic separation process involves two types of elution modes of operation: isocratic elution and gradient elution. Isocratic elution refers to an operation mode of eluting and separating different target components according to different elution rates of an eluent on different components to be separated adsorbed on a chromatographic column stationary phase, wherein the composition proportion and the flow rate of the eluent are kept constant in the elution and separation process. Isocratic elution has the advantage of simple operation process, and is the most widely applied chromatographic separation method at present. However, when the difference in chemical properties of the components to be separated is small, the isocratic elution separation has limitations such as a long separation period and a small throughput. Gradient elution is also known as gradient elution or program elution. The concentration ratio of the mobile phase is continuously changed in the same separation period, and the separation period is called gradient elution. Gradient elution chromatographic separation principle: the eluent is composed of several solvents with different polarities, and the polarity of the eluent is changed by changing the proportion of the solvents in the eluent, so that each component flowing out has a proper capacity factor, and all the components in the material can be optimally separated in the shortest time. Advantages of gradient elution: 1. the separation period is shortened; 2. the separation capacity is improved; 3. is suitable for separating materials with complicated components and difficult separation. The traditional chromatographic separation technology combined with the isocratic elution method is not suitable for materials with very similar physicochemical properties and difficult components to separate, so that the separation period is long, the treatment capacity is small, the development of the chromatographic separation technology is severely limited, and the chromatographic separation mode of gradient elution is rarely reported.
The utility model provides a separating device for gradient elution, which aims to solve the problems in the prior art.
SUMMERY OF THE UTILITY MODEL
In view of the above problems, the present invention provides a gradient elution chromatographic separation device, which has a reasonable structural design, a low cost, and a simple operation, and is suitable for separating materials with complex components and difficult separation.
In order to realize the purpose of the utility model, the utility model is realized by the following technical scheme: the utility model provides a chromatographic fractionation device of gradient elution, includes rack and mounting bracket, rack upper portion from left to right is equipped with raw materials measuring pump, eluent A measuring pump, eluent B measuring pump, motor, eluent C measuring pump in proper order, the rack lower part is equipped with the constant temperature basin, constant temperature basin the inside from left to right is equipped with material container, eluent A container, eluent B container and eluent C container in proper order, the mounting bracket on from left to right be equipped with first chromatographic fractionation post, second chromatographic fractionation post, third chromatographic fractionation post and fourth chromatographic fractionation post in proper order, be equipped with circulation intercommunication mechanism between first chromatographic fractionation post, second chromatographic fractionation post, third chromatographic fractionation post and the fourth chromatographic fractionation post.
The further improvement lies in that: and the upper part of the eluent C container is provided with a motor, the inner part of the eluent C container is provided with a stirring paddle, and the motor is connected with the stirring paddle.
The further improvement lies in that: the eluent A container is connected with an eluent conveying pipe and an eluent A metering pump and is communicated with an eluent C container, and the eluent B container is connected with an eluent conveying pipe and an eluent B metering pump and is communicated with the eluent C container.
The further improvement lies in that: and the upper part of the eluent C container is connected with an eluent C metering pump and an eluent conveying pipe which are communicated with the upper part of the first chromatographic separation column.
The utility model has the beneficial effects that: the utility model has reasonable structure design, low cost and simple and convenient operation, and in the actual operation, the ratio of the eluent in the container of the eluent C is increased in a gradient way by controlling the flow rates of the metering pump of the eluent A and the metering pump of the eluent B, so that the whole separation device achieves the optimal separation effect.
Drawings
The utility model is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic diagram of a gradient elution chromatographic separation apparatus of the present invention.
Wherein: 1. a rack; 2. a constant temperature water tank; 3. a material container; 4. eluent A container; 5. eluent B container; 6. eluent C container; 7. a raw material metering pump; 8. eluent A metering pump; 9. eluent B metering pump; 10. eluent C metering pump; 11. a motor; 12. a stirring paddle; 13. a raw material conveying pipe; 14. an eluent conveying pipe; 15. a first chromatographic separation column; 16. a second chromatographic separation column; 17. a third chromatographic separation column; 18 a fourth chromatographic separation column; 19. a delivery valve; 20. a drain valve; 21. a circulation pipe; 22. a mounting frame; 23. clamping a hoop; 24. a material collecting barrel.
Detailed Description
In order to further understand the present invention, the following detailed description will be made with reference to the following examples, which are only used for explaining the present invention and are not to be construed as limiting the scope of the present invention.
According to fig. 1, this embodiment provides a gradient elution chromatographic fractionation device, including rack 1 and mounting bracket 22, rack 1 upper portion from left to right is equipped with raw materials measuring pump 7, eluent a measuring pump 8, eluent B measuring pump 9, motor 11, eluent C measuring pump 10 in proper order, rack 1 lower part is equipped with constant temperature basin 2, constant temperature basin the inside from left to right is equipped with material container 3, eluent a container 4, eluent B container 5 and eluent C container 6 in proper order, mounting bracket 22 on from left to right be equipped with first chromatographic fractionation column 15, second chromatographic fractionation column 16, third chromatographic fractionation column 17 and fourth chromatographic fractionation column 18 in proper order, be equipped with circulation communicating mechanism between first chromatographic fractionation column 15, second chromatographic fractionation column 16, third chromatographic fractionation column 17 and the fourth chromatographic fractionation column 18.
And the upper part of the eluent C container 6 is provided with a motor 11, the inner part of the eluent C container is provided with a stirring paddle 12, and the motor 11 is connected with the stirring paddle 12. The eluent C is a mixed solution of eluent a and eluent B, and the motor 11 and the stirring paddle 12 function to uniformly mix the solution in the container 6 of eluent C.
The eluent A container 4 is connected with an eluent conveying pipe 14 and an eluent A metering pump 8 and is communicated with an eluent C container 6, and the eluent B container 5 is connected with an eluent conveying pipe 14 and an eluent B metering pump 9 and is communicated with the eluent C container 6. The flow rates of the eluent a metering pump 8 and the eluent B metering pump 9 at different time periods are adjusted so that the proportion of eluent in the container of eluent C changes and the eluent proportion rises in a gradient over time.
The upper part of the raw material container 3 is connected with a raw material metering pump 7 and a raw material conveying pipe 13 which are communicated with the upper part of a first chromatographic separation column 15, and the upper part of the eluent C container 6 is connected with an eluent C metering pump 10 and an eluent conveying pipe 14 which are communicated with the upper part of the first chromatographic separation column 15.
The circulating communication mechanism comprises a circulating pipe 21 and a conveying valve 19, the lower end of the first chromatographic separation column 15 and the upper end of the second chromatographic separation column 16, the lower end of the second chromatographic separation column 16 and the upper end of the third chromatographic separation column 17, the lower end of the third chromatographic separation column 17 and the upper end of the fourth chromatographic separation column 18, the lower end of the fourth chromatographic separation column 18 and the upper end of the first chromatographic separation column 15 are connected in series through the circulating pipe 21, and the circulating pipe 21 is provided with the conveying valve 19.
The mounting rack 22 is sequentially provided with a first chromatographic separation column 15, a second chromatographic separation column 16, a third chromatographic separation column 17 and a fourth chromatographic separation column 18 through a clamp 23.
The lower ends of the first chromatographic separation column 15, the second chromatographic separation column 16, the third chromatographic separation column 17 and the fourth chromatographic separation column 18 are provided with a liquid discharge valve 20, and a material collecting barrel 24 is arranged below the liquid discharge valve 20. The first, second, third, and fourth chromatographic separation columns 15, 16, 17, and 18 are fixed to a mounting frame 22 using clamps 23.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the utility model as claimed. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (4)
1. The utility model provides a chromatographic fractionation device of gradient elution, its characterized in that includes rack (1) and mounting bracket (22), rack (1) upper portion from left to right is equipped with raw materials measuring pump (7), eluent A measuring pump (8), eluent B measuring pump (9), motor (11), eluent C measuring pump (10) in proper order, rack (1) lower part is equipped with constant temperature basin (2), constant temperature basin the inside from left to right is equipped with material container (3), eluent A container (4), eluent B container (5) and eluent C container (6) in proper order, mounting bracket (22) on from left to right be equipped with first chromatographic fractionation post (15), second chromatographic fractionation post (16), third chromatographic fractionation post (17) and fourth chromatographic fractionation post (18) in proper order, first chromatographic fractionation post (15), second chromatographic fractionation post (16), A circulating communication mechanism is arranged between the third chromatographic separation column (17) and the fourth chromatographic separation column (18).
2. A gradient elution chromatographic separation device as set forth in claim 1 wherein: eluent C container (6) upper portion is equipped with motor (11), and inside is equipped with stirring rake (12), motor (11) and stirring rake (12) are connected.
3. A gradient elution chromatographic separation device as set forth in claim 1 wherein: the eluent A container (4) is connected with an eluent conveying pipe (14) and an eluent A metering pump (8) and is communicated with an eluent C container (6), and the eluent B container (5) is connected with an eluent conveying pipe (14) and an eluent B metering pump (9) and is communicated with the eluent C container (6).
4. A gradient elution chromatographic separation device as set forth in claim 1 wherein: and the upper part of the material container (3) is connected with a raw material metering pump (7) and a raw material conveying pipe (13) and is communicated with the upper part of the first chromatographic separation column (15), and the upper part of the eluent C container (6) is connected with an eluent C metering pump and an eluent conveying pipe and is communicated with the upper part of the first chromatographic separation column (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121874702.4U CN216309887U (en) | 2021-08-02 | 2021-08-02 | Chromatographic separation device for gradient elution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121874702.4U CN216309887U (en) | 2021-08-02 | 2021-08-02 | Chromatographic separation device for gradient elution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216309887U true CN216309887U (en) | 2022-04-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121874702.4U Active CN216309887U (en) | 2021-08-02 | 2021-08-02 | Chromatographic separation device for gradient elution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216309887U (en) |
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2021
- 2021-08-02 CN CN202121874702.4U patent/CN216309887U/en active Active
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