CN201583529U - Ultrasonic standing wave preparative liquid chromatogram - Google Patents
Ultrasonic standing wave preparative liquid chromatogram Download PDFInfo
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- CN201583529U CN201583529U CN2009202744858U CN200920274485U CN201583529U CN 201583529 U CN201583529 U CN 201583529U CN 2009202744858 U CN2009202744858 U CN 2009202744858U CN 200920274485 U CN200920274485 U CN 200920274485U CN 201583529 U CN201583529 U CN 201583529U
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Abstract
The utility model relates to an ultrasonic standing wave preparative liquid chromatogram, a device of which comprises a solvent system tank, a solute component tank, a constant-flow pump, a fluid input valve, an ultrasonic standing wave chromatographic system, a chromatographic detector, a chromatogram working station, an automatic control unit, a distillate collection tank, an extraction liquid tank and the like. A solvent system comprises immiscible two phase solvents containing the solute component; in the ultrasonic standing wave chromatographic system, the difference of the distribution ratios of the solute component in the two-phase solvent system can be enlarged for a plurality of times, thereby achieving the purpose of separation; and as the ultrasonic standing wave chromatographic system continuously inputs standing wave field separation, the ultrasonic standing wave preparative liquid chromatogram has large processing amount, and is suitable for the fine separation of chemistry, chemical industry, biology, pharmacy, food and the like.
Description
Affiliated technical field
The utility model relates to liquid phantom preparing chromatogram, particularly a kind of ultrasonic standing wave liquid phantom preparing chromatogram.
Background technology
Known adverse current liquid phantom preparing chromatogram be a kind of need not solid-state supporter or the liquid liquid distribution chromatography of carrier, characteristic feature is that immiscible stationary phase and moving phase is made countercurrent movement in detachment process, and the partition factor of solute component in two-phase is different and obtain separating.
Relate to a kind of no-load body fluid liquid at CN2299318 and distribute the adverse current chromatogram instrument, stationary phase in the spiral tube obtains stable retention in the motion of revolution in hollow shaft is made surface level and the rotation in the axis of rotation is made vertical plane, improved the sample size of sample, on the placement framework of instrument, arranged two separative elements, the separator tube capacity of the instrument that accounts for same spatial volume is amplified.
CN2466653 is a kind of many separating columns high-speed counter-current chromatograph, comprise: separating column, transmission motor, and the sun wheel and the support that are used for the separating column installing and locating, it is characterized in that: sun wheel is provided with three separating columns, separating column respectively with planet circular system on the coaxial installation of star gear; Be arranged on be equipped with on the transition axis between the separating column can with the transition gear of the coaxial star gearing mesh of separating column running.Separating column with sun wheel in fixation hollow axle revolution, to make planetary rotation in the same way, realized static equilibrium and transient equilibrium with speed, utilized the space, increased the capacity of instrument.
CN1289922 discloses a kind of biochemical Process for preparing liquid-phase chromatograph, comprise the moving phase basin, the chromatographic work station of pump, valve, chromatographic column, detecting device, gatherer and the control data collection and the processing of injection moving phase etc., wherein, described pump is normal pressure chromatogram peristaltic pump and middle pressure chromatogram titanium pump, and described chromatographic column is the titanium alloy chromatographic column, and described gatherer is an automatic fraction collector, and being equipped with ten channel valve as collecting by the peak, described chromatographic work station is realized the control to pump, valve.
CN2583672 is a kind of high-speed counter-current chromatograph, the central axis of its instrument and spiral tube from all vertical setting of rotation axis, transmission by gearing mesh, make and separate with spiral tube in the revolution of centre axis, do the rotation of equidirectional same angular velocity around self axis, the quantity of spiral tube can be for a plurality of.This set has utilized the effect of gravity field, has kept the characteristics that existing high-speed counter-current chromatograph can be realized the high retention of stationary phase, big flow wash-out.
The ZL200720142649.2 high speed multidimensional stream ultrasonic liquid phantom preparing chromatogram, its equipment is by dicyandiamide solution jar, solute component jar, mixing tank, infusion pump, tubulose chromatographic column, multidimensional fluid element, ultrasonic pulse clarification element, electromagnetic oscillation cyclone, chromatographic detector, registering instrument, control module, component holding tank, compositions such as raffinate flow container.
The utility model content
In principle, above-mentioned several liquid chromatographies may be used to preparation technology, but it is the problem that solid-liquid chromatography is difficult to solve that high pressure liquid chromatography belongs to the irreversible adsorption sex change of Separation of Solid and Liquid chromatogram solute component on stationary phase, the architectural feature of above-mentioned adverse current liquid chromatography, the operating cycle is long in commercial production.High speed multidimensional stream ultrasonic liquid phantom preparing chromatogram complex structure operating process technological parameter is more, and the present invention can overcome above-mentioned deficiency.
The purpose of this utility model is to provide a kind of ultrasonic standing wave liquid phantom preparing chromatogram, this device is to utilize the dicyandiamide solution of immiscible solvent composition and solute component to produce distribution and coalescence in the ultrasonic standing wave field, because partition factor difference in the two-phase of solute component in dicyandiamide solution, this species diversity is through repeatedly enlarging, and solute component is separated fast.
The utility model solves technical scheme that its technical matters adopts: 1. equipment is made up of solvent tank, solute component jar, constant flow pump, solute fillup valve, standing wave chromatographic system, diverting valve, detecting device, chromatographic work station, registering instrument, automatic control system, fraction collection jar and raffinate NaOH solution tank NaOH etc., the standing wave chromatographic system assembles a plurality of ultrasonic standing wave separation vessels, and each ultrasonic standing wave separation vessel is made up of standing wave device and class standing wave device.2. during equipment operation, select immiscible two-phase solvent to form dicyandiamide solution, with components dissolved to be separated in the stationary phase solvent, pump in the netted standing wave chromatographic system of branch, open the ultrasonic standing wave separation vessel, then mobile phase solvent is pumped into the netted standing wave chromatographic system of branch, in the ultrasonic standing wave separation vessel owing to there is not the directional energy stream of energy in the stationary wave vibration process, but energy can be between antinode and node local flow, the mobile moving phase and stationary phase and solute component of promoting of local energy is repeatedly collided, repeated dispensing, moving phase and stationary phase difference coalescence are at antinode place and node place, become metastable state to cross two metastable states between transducer regularly through class stationary field condition lower class standing wave again, make and be trapped in that the moving phase of coalescence and the class standing wave of stationary phase passive movement are taken away at a distance of half-wavelength everywhere, concentrate on the both sides of transducer respectively, solute component is separated, detect and use the registering instrument record through chromatographic detector, after the chromatographic work station deal with data automatic control unit is provided the chromatographic running parameter of signal controlling, separation component enters fraction collection jar and raffinate basin respectively
Description of drawings
The present invention is further described below in conjunction with drawings and Examples
Fig. 1 is a ultrasonic standing wave liquid phantom preparing chromatogram synoptic diagram
Fig. 2 is a ultrasonic standing wave cyclone separator arrangement synoptic diagram
Fig. 3 is the netted ultrasonic standing wave chromatographic system structural representation of branch
11. netted standing wave chromatographic system 12. chromatographic detectors 13. chromatographic work stations 14 fraction collection tanks 15. raffinate collecting tanks 16. ultrasonic standing wave separators 17. ultrasonic powers 18. transducer oscillators 19. XPMs 20. ultrasonic standing wave separator fluid intake 21.22. ultrasonic standing wave separator fluid issuing 23.24. pump 25.26. valves 27. automatic control units of 1.2. dicyandiamide solution tank 3. solute component tank 4.5.6 constant flow pump 7.8.9. valves 10. fluid transfer valves among the figure
Embodiment
In Fig. 1, dicyandiamide solution jar (1) is connected with liquid transfer valve (10) by constant flow pump (4) and valve (9), solution system jar (2) is connected with liquid transfer valve (10) by constant flow pump (5) and valve (7), solute component jar (3) is connected with liquid transfer valve (10) by constant flow pump (6) and valve (8), liquid transfer valve (10) is connected with the netted ultrasonic standing wave chromatographic system of branch (11), the netted ultrasonic standing wave chromatographic system of branch (11) is made up of many groups ultrasound wave separation vessel (16), the cut outlet (21) of the netted ultrasonic standing wave chromatographic system of branch (11) is connected with chromatographic detector (12), the raffinate outlet (22) of the netted ultrasonic standing wave chromatographic system of branch (11) is connected with raffinate holding tank (15) through valve (25), chromatographic detector (12) one ends are connected with fraction collection jar (14) and the other end is connected with chromatographic work station (13) through valve (26), chromatographic work station (13) is connected with automatic control unit (27), automatic control unit (27) is connected with valve (9) with valve (7) respectively, and automatic control unit (27) is connected with fluid transfer valve (10).
In Fig. 2, the both sides of ultrasonic standing wave separation vessel (16) are provided with ultrasonic power (17) and are connecting transducer oscillator (18), in the centre of ultrasonic standing wave separation vessel (16) a φ 0.1mm-φ 10mm XPM (19) is arranged, a ultrasonic standing wave separator liquid inlet (20) is arranged at ultrasound wave separation vessel (16) top, two ultrasonic standing wave separator liquid outlets (21,22) are arranged in ultrasonic standing wave separation vessel (16) bottom
In Fig. 3, the netted standing wave chromatographic system of branch (11) is by netted being formed by connecting of a plurality of ultrasonic standing wave separation vessels (16) branch.
Embodiment 1:
500mm * 2000mm dicyandiamide solution jar (1) and configuration flow 10.0mL/min-1000mL/min, the constant flow pump of linear velocity 1m/s-50m/s (5) is through φ 2.0mm-φ 20mm, and valve (9) is connected with φ 2.0mm-φ 50mm fluid transfer valve (10) with pipeline; φ 500mm * 2000mm dicyandiamide solution jar (2) and configuration flow 50mL/min-1000mL/min, the constant flow pump of linear velocity 0.5m/s-100m/s (4) is connected with 5.0mm-500mm fluid transfer valve (10) with pipeline through φ 2.0mm-φ 50mm valve (7), φ 500mm * 1000mm solute component jar (3) and configuration flow 10mL/min-200mL/min, the constant flow pump of linear velocity 0.5m/s-100m/s (6) is connected with φ 5.0mm-φ 200mm fluid transfer valve (10) with pipeline through φ 1.0mm-φ 20mm valve (8), fluid transfer valve (10) is connected with automatic control unit (26) with φ 0.1mm-φ 2.0mm copper conductor, fluid transfer valve (10) is connected with the ultrasonic standing wave separation vessel fluid intake (20) of the netted ultrasonic standing wave chromatographic system of branch (11) with φ 5.0mm-φ 200mm pipeline, the netted ultrasonic standing wave chromatographic system of branch (11) is made up of 1 group of-1023 groups of ultrasonic standing wave separation vessel (16), ultrasonic standing wave separation vessel fluid egress point (21) is connected with chromatographic detector (12) with φ 0.1mm-φ 3.0mm shunt conduit, the available UV-detector of chromatographic detector (12) or use the differential detecting device, chromatographic detector (12) is connected with chromatographic work station (13) with φ 0.1mm-φ 2.0mm copper conductor, chromatographic work station (13) is connected with automatic control unit (26) with φ 0.1mm-φ 2.0mm copper conductor, automatic control unit (26) with φ 0.1mm-φ 2.0mm copper conductor respectively with valve (7), valve (8), valve (9) connects, ultrasonic standing wave separation vessel fluid egress point (21) is connected with valve (26) and is connected with the fraction collection jar (14) of φ 500mm * 1000mm-φ 1000mm * 10000mm by pump (23) with φ 10.0mm-φ 300mm pipeline, and ultrasonic standing wave separator outlet (22) is connected and is pumped into by pump (24) the raffinate holding tank (15) of φ 1000mm * 3000mm with valve (25) with φ 5.0mm-φ 300mm pipeline.
Embodiment 2:
φ 100mm * 1000mm-φ 500mm * 15000mm dicyandiamide solution jar (1) and configuration flow 100mL/min-5000mL/min, the constant flow pump of linear velocity 1m/s-50m/s (5) is connected with φ 2.0mm-φ 20.00mm fluid transfer valve (10) with pipeline through φ 2.0mm-φ 100mm valve (9), φ 500mm * 1000mm-φ 1000mm * 2000mm dicyandiamide solution jar (2) is connected with φ 5.0mm-2000mm fluid transfer valve (10) with pipeline through φ 2.0mm-φ 100mm valve (7) with the constant flow pump (4) of configuration flow 100mL/min-2000mL/min linear velocity 0.5m/s-100m/s, φ 500mm * 1000mm-φ 500mm * 2000mm solute component jar (3) and configuration flow 10mL/min-1000mL/min, the constant flow pump of linear velocity 0.5m/s-100m/s (6) is connected with φ 5.0mm-2000mm fluid transfer valve (10) with pipeline through φ 1.0mm-φ 50mm valve (8), fluid transfer valve (10) is connected with automatic control unit (27) with φ 0.1mm-φ 2.0mm copper conductor, fluid transfer valve (10) is connected with the ultrasonic standing wave separation vessel fluid intake (20) of the netted standing wave chromatographic system of branch (11) with φ 5.0mm-φ 500mm pipeline, the netted standing wave chromatographic system of branch (11) is made up of 1 group of-511 groups of ultrasonic standing wave separation vessel (16), ultrasonic standing wave separation vessel fluid egress point (21) is connected with chromatographic detector (12) with φ 0.1-5.0mm shunt conduit, the available UV-detector of chromatographic detector (12) or use the differential detecting device, chromatographic detector (12) is connected with chromatographic work station (13) with φ 0.1mm-φ 2.0mm copper conductor, chromatographic work station (13) with φ 0.1mm-φ 2.0mm copper conductor respectively with valve (7), valve (8), valve (9) connects, ultrasonic standing wave separation vessel fluid egress point (21) is connected with valve (26) and is connected with the fraction collection jar (14) of φ 500mm * 1000mm-φ 2000mm * 15000mm by pump (23) with φ 10.00mm-φ 500mm pipeline, and ultrasonic standing wave separator outlet (22) is connected and is pumped into by pump (24) the raffinate holding tank (15) of φ 2000mm * 5000mm with valve (25) with φ 5.0mm-φ 500mm pipeline.
Claims (3)
1. ultrasonic standing wave liquid phantom preparing chromatogram, it is characterized in that: dicyandiamide solution jar (1) is connected with liquid transfer valve (10) by constant flow pump (4) and valve (9), dicyandiamide solution jar (2) is connected with liquid transfer valve (10) by constant flow pump (5) and valve (7), solute component jar (3) is connected with liquid transfer valve (10) by constant flow pump (6) and valve (8), liquid transfer valve (10) is connected with the netted ultrasonic standing wave chromatographic system of branch (11), the netted ultrasound wave standing wave chromatographic system of branch (11) is made up of many groups ultrasonic standing wave separation vessel (16), the cut outlet (21) of the netted ultrasonic standing wave chromatographic system of branch (11) is connected with chromatographic detector (12), the raffinate outlet (22) of the netted ultrasonic standing wave chromatographic system of branch (11) is connected with raffinate holding tank (15) through valve (25), chromatographic detector (12) one ends are connected with fraction collection jar (14) through valve (26), the other end is connected with chromatographic work station (13), chromatographic work station (13) is connected with automatic control unit (27), automatic control unit (27) is connected with valve (9) with valve (7) respectively, automatic control unit (27) connects, automatic control unit (27) is connected with valve (9) with valve (7) respectively, and automatic control unit (27) is connected with fluid transfer valve (10).
2. ultrasound wave liquid phase preparative chromatography according to claim 1, it is characterized in that: the both sides of ultrasound wave separation vessel (16) are provided with ultrasonic power (17) and are connecting transducer oscillator (18), in the centre of ultrasonic standing wave separation vessel (16) XPM (19) is arranged, have a fluid intake (20) two ultrasonic standing wave separator outlets (21.22) to be arranged in the bottom of ultrasonic standing wave separation vessel (16) at the top of ultrasound wave separation vessel (16)
3. ultrasonic standing wave liquid phantom preparing chromatogram according to claim 1 is characterized in that: the netted standing wave chromatographic system of branch (11) is made up of 1 group of-1023 groups of ultrasonic standing wave separation vessel (16).
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009202744858U CN201583529U (en) | 2009-12-07 | 2009-12-07 | Ultrasonic standing wave preparative liquid chromatogram |
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| CN2009202744858U CN201583529U (en) | 2009-12-07 | 2009-12-07 | Ultrasonic standing wave preparative liquid chromatogram |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102078594A (en) * | 2011-01-14 | 2011-06-01 | 哈尔滨工业大学 | Application of toad peptide antibiotics in preparing drugs for treating anal fistula |
| CN102078595A (en) * | 2011-01-14 | 2011-06-01 | 哈尔滨工业大学 | Application of toad peptide antibiotic for preparing medicines for curing tinea manus and pedis |
| CN102657708A (en) * | 2012-06-02 | 2012-09-12 | 哈尔滨工业大学 | Anti-influenza medicine with immune regulating function and preparation method thereof |
-
2009
- 2009-12-07 CN CN2009202744858U patent/CN201583529U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102078594A (en) * | 2011-01-14 | 2011-06-01 | 哈尔滨工业大学 | Application of toad peptide antibiotics in preparing drugs for treating anal fistula |
| CN102078595A (en) * | 2011-01-14 | 2011-06-01 | 哈尔滨工业大学 | Application of toad peptide antibiotic for preparing medicines for curing tinea manus and pedis |
| CN102078595B (en) * | 2011-01-14 | 2012-11-07 | 哈尔滨工业大学 | Application of toad peptide antibiotic for preparing medicines for curing tinea manus and pedis |
| CN102657708A (en) * | 2012-06-02 | 2012-09-12 | 哈尔滨工业大学 | Anti-influenza medicine with immune regulating function and preparation method thereof |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100915 Termination date: 20141207 |
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| EXPY | Termination of patent right or utility model |