CN216050992U

CN216050992U - Sample collection system is used in detection of environmental bacteria content

Info

Publication number: CN216050992U
Application number: CN202122593447.2U
Authority: CN
Inventors: 徐道俊; 何志宇; 周巧; 周玲; 司俊波; 李美玲; 刘成
Original assignee: Yunnan Precision Inspection Co ltd
Current assignee: Yunnan Precision Inspection Co ltd
Priority date: 2021-10-27
Filing date: 2021-10-27
Publication date: 2022-03-15
Anticipated expiration: 2031-10-27

Abstract

The utility model discloses a sample collecting device for detecting the bacteria content of an environment, which comprises a first collecting chamber, a second collecting chamber and a third collecting chamber; a gas channel to be detected is arranged on the side wall of the first collection chamber; the bottom of the first collection chamber is provided with a first air flow channel; the bottom of the first air flow channel is connected with a second collection chamber; the first collection chamber and the second collection chamber are communicated through a first air flow channel; a first air pump is arranged on the first air flow channel; the utility model has simple use and accurate measurement, and is suitable for popularization and application.

Description

Sample collection system is used in detection of environmental bacteria content

Technical Field

The utility model relates to a sample collection device for detecting the bacteria content in an environment.

Background

Accurate sampling and detection of the number of microorganisms in the air are one of the important indexes for evaluating the environmental sanitation quality and controlling pollution measures. The conventional detection methods are an impact method and a natural sedimentation method; the plate sedimentation method is the simplest and most common method in China. It is to make the microorganism particles in the air naturally settle on the nutrient agar plate, and then the colony number obtained by culturing on the plate. And converting the number of the bacteria floating in the air according to an austenite formula. The method is simple, but the accuracy is poor. The impaction method is to use external force to make microorganism particles suspended in air to impact on the surface of solid culture medium. Compared with the flat plate sedimentation method, the impact method has more accurate effect than the sedimentation method.

Before a collision method is adopted for a microorganism detection experiment, a sample is sampled, and the existing collision method sampler directly impacts microorganism ions on the surface of a solid culture medium, so that the bacteria on the surface and the culture are not uniformly dispersed, and errors are brought to the detection result. For the above reasons, there is a need to optimize existing sampling devices to more uniformly disperse microbial particles on the surface of the culture medium.

Disclosure of Invention

The utility model aims to provide a sample collecting device for detecting the bacteria content in an environment, which is used for solving the problems in the prior art.

In order to solve the technical problems, the utility model adopts the following technical scheme:

a sample collecting device for detecting the bacteria content in the environment comprises a first collecting chamber 1, a second collecting chamber 2 and a third collecting chamber 3;

a gas channel 4 to be detected is arranged on the side wall of the first collection chamber 1;

the bottom of the first collection chamber 1 is provided with a first air flow channel 5; the bottom of the first air flow channel 5 is connected with a second collection chamber 2; the first collection chamber 1 and the second collection chamber 2 are communicated through a first air flow channel 5; the first air flow channel 5 is provided with a first air pump 6;

a filter screen 7 is arranged in the second collection chamber 2;

the bottom of the second collection chamber 2 is provided with a second airflow channel 8; the bottom of the second airflow channel 8 is provided with a third collection chamber 3; the second collection chamber 2 and the third collection chamber 3 are communicated through a second airflow channel 8;

the bottom of the second airflow channel 8 is arranged in the middle of the third collection chamber 3; the top of the third collecting chamber 3 is respectively provided with a liquid inlet 9 and a gas outlet 10; the bottom of the third collection chamber 3 is provided with a liquid outlet 20.

Further, a third airflow channel 11 is arranged on the side wall of the first collection chamber 1; a second air pump 12 is arranged on the third air flow channel 11; the gas channel 4 to be measured is provided with a valve (21).

Further, the second collection chamber 2 comprises an upper cavity 13 and a lower cavity 14; the edges of the upper cavity 13 and the lower cavity 14 are respectively and correspondingly provided with a fixed flange 15; the fixed flange 15 is provided with a fastening bolt 16; the upper cavity 13 and the lower cavity 14 are movably connected through a fastening bolt 16; the filter screen 7 is detachably arranged on the inner wall of the lower cavity 14.

Further, an elastic sealing gasket 17 is arranged between the fixing flanges 15 of the upper cavity 13 and the lower cavity 14.

Further, an airflow nozzle 18 is arranged at the bottom of the second airflow channel 8; the air flow nozzle 18 is provided with a plurality of air hole channels 19 which are uniformly distributed.

Further, the second air flow channel 8 is configured to be wide at the top and narrow at the bottom.

Compared with the prior art, the utility model has at least one of the following beneficial effects:

the application discloses sample collection system is used in detection of environmental bacteria content, and the operation flow of this application is:

cleaning the device by airflow:

connecting a pure and sterile gas source with a gas channel 4 to be detected, then starting a first gas pump 6, and discharging gas along a first collecting chamber 1, a first gas flow channel 5, a second collecting chamber 2, a second gas flow channel 8, a third collecting chamber 3 and a gas outlet 10 to ensure that the whole equipment is kept in a sterile state. The valves and air pump are then closed.

Ordinary sampling:

and opening the first air pump 6 and the valve 21, introducing liquid with the function of adsorbing microorganisms into the third collection chamber 3 through the liquid inlet 9, enabling the air flow nozzle 18 to submerge the surface of the liquid, retaining the microorganisms in the liquid along with the introduction of the gas, and discharging the gas from the gas outlet 10, thereby obtaining the types of the microorganisms.

Accurate sampling:

closing the first air pump 6, and opening the valve 21 and the second air pump 12 to store the gas to be detected in the first collecting chamber 1; then the valve 21 and the second air pump 12 are closed, the first air pump 6 is opened, the volume of the measurement air is obtained by measuring the vacuum degree of the first collection chamber 1, then the remaining sample to be measured in the first collection chamber 1 is emptied, sterile air is continuously discharged into the second collection chamber 2 until all the air in the second collection chamber 2 is replaced into the third collection chamber 3, and thus the number of microorganisms is obtained.

In conclusion, the utility model has the advantages of simple use, accurate measurement and suitability for popularization and application.

Drawings

FIG. 1 is a schematic structural diagram of the present invention.

FIG. 2 is a schematic bottom structure view of the air flow nozzle of the present invention.

In the figure:

a first collection chamber 1; a second collection chamber 2; a third collection chamber 3; a gas channel to be measured 4; a first air flow passage 5; a first air pump 6; a filter screen 7; a second airflow passage 8; a liquid inlet 9; an air outlet 10; a third airflow passage 11; the second air pump 12; an upper cavity 13; a lower cavity 14; a fixing flange 15; a fastening bolt 16; an elastic sealing gasket 17; an air flow nozzle 18; an air hole passage 19; a liquid outlet 20; a valve (21).

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the utility model and are not intended to limit the utility model.

As shown in the figure:

example 1:

a filter screen 7 is arranged in the second collection chamber 2;

Example 2:

on the basis of example 1:

a third airflow channel 11 is arranged on the side wall of the first collection chamber 1; a second air pump 12 is arranged on the third air flow channel 11; the gas channel 4 to be measured is provided with a valve 21.

Example 3:

on the basis of examples 1-2:

the second collection chamber 2 comprises an upper cavity 13 and a lower cavity 14; the edges of the upper cavity 13 and the lower cavity 14 are respectively and correspondingly provided with a fixed flange 15; the fixed flange 15 is provided with a fastening bolt 16; the upper cavity 13 and the lower cavity 14 are movably connected through a fastening bolt 16; the filter screen 7 is detachably arranged on the inner wall of the lower cavity 14.

Example 4:

on the basis of examples 1 to 3:

an elastic sealing gasket 17 is arranged between the fixing flanges 15 of the upper cavity 13 and the lower cavity 14.

Example 5:

on the basis of examples 1 to 4:

an airflow spray head 18 is arranged at the bottom of the second airflow channel 8; the air flow nozzle 18 is provided with a plurality of air hole channels 19 which are uniformly distributed.

Example 6:

on the basis of examples 1 to 5:

the second air flow channel 8 is arranged in a structure with a wide top and a narrow bottom.

Reference throughout this specification to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment described generally in this application. The appearances of the same phrase in various places in the specification are not necessarily all referring to the same embodiment. Further, when a particular feature, structure, or characteristic is described in connection with any embodiment, it is submitted that it is within the scope of the utility model to effect such feature, structure, or characteristic in connection with other embodiments.

Although the utility model has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the spirit and scope of the principles of this disclosure. More specifically, various variations and modifications are possible in the component parts and/or arrangements of the subject combination arrangement within the scope of the disclosure, the drawings and the appended claims. In addition to variations and modifications in the component parts and/or arrangements, other uses will also be apparent to those skilled in the art.

Claims

1. The sample collection device for detecting the bacteria content in the environment is characterized by comprising a first collection chamber (1), a second collection chamber (2) and a third collection chamber (3);

a gas channel (4) to be detected is arranged on the side wall of the first collection chamber (1);

the bottom of the first collection chamber (1) is provided with a first air flow channel (5); the bottom of the first air flow channel (5) is connected with a second collection chamber (2); the first collection chamber (1) and the second collection chamber (2) are communicated through a first air flow channel (5); a first air pump (6) is arranged on the first air flow channel (5);

a filter screen (7) is arranged in the second collection chamber (2);

a second airflow channel (8) is arranged at the bottom of the second collection chamber (2); a third collecting chamber (3) is arranged at the bottom of the second airflow channel (8); the second collection chamber (2) and the third collection chamber (3) are communicated through a second airflow channel (8);

the bottom of the second airflow channel (8) is arranged in the middle of the third collection chamber (3); the top of the third collecting chamber (3) is respectively provided with a liquid inlet (9) and a gas outlet (10); a liquid outlet (20) is arranged at the bottom of the third collection chamber (3).

2. The environmental bacteria content detection sample collection device according to claim 1, wherein: a third airflow channel (11) is arranged on the side wall of the first collection chamber (1); a second air pump (12) is arranged on the third air flow channel (11); a valve (21) is arranged on the gas channel (4) to be measured.

3. The environmental bacteria content detection sample collection device according to claim 1, wherein: the second collection chamber (2) comprises an upper cavity (13) and a lower cavity (14); fixing flanges (15) are correspondingly arranged at the edges of the upper cavity (13) and the lower cavity (14) respectively; the fixed flange (15) is provided with a fastening bolt (16); the upper cavity (13) and the lower cavity (14) are movably connected through a fastening bolt (16); the filter screen (7) is detachably arranged on the inner wall of the lower cavity (14).

4. The environmental bacteria content detection sample collection device according to claim 3, wherein: an elastic sealing gasket (17) is arranged between the fixing flanges (15) of the upper cavity (13) and the lower cavity (14).

5. The environmental bacteria content detection sample collection device according to claim 1, wherein: an airflow spray head (18) is arranged at the bottom of the second airflow channel (8); the airflow nozzle (18) is provided with a plurality of air hole channels (19) which are uniformly distributed.

6. The environmental bacteria content detection sample collection device according to claim 1, wherein: the second air flow channel (8) is arranged in a structure with a wide upper part and a narrow lower part.