CN215975765U - Test tube constant temperature equipment, cell recovery equipment and cell recovery instrument - Google Patents
Test tube constant temperature equipment, cell recovery equipment and cell recovery instrument Download PDFInfo
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- CN215975765U CN215975765U CN202122255372.7U CN202122255372U CN215975765U CN 215975765 U CN215975765 U CN 215975765U CN 202122255372 U CN202122255372 U CN 202122255372U CN 215975765 U CN215975765 U CN 215975765U
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Abstract
The utility model provides a test tube constant temperature device, cell resuscitation equipment and a cell resuscitation instrument, and relates to the technical field of medical experimental equipment, wherein the device comprises two heating modules, two temperature detection modules, an infrared temperature measurement sensor and a suction electromagnetic valve; a test tube groove for placing a test tube is arranged in the device; the two heating modules are respectively arranged on two sides of the test tube groove and used for heating the test tube; the two temperature detection modules are respectively used for detecting the temperatures of the two heating modules; the infrared temperature measuring sensor is used for detecting the temperature of the test tube; the actuation solenoid valve actuation after the test tube is put into the test-tube groove for guarantee the test tube respectively with two heating module fixed contact, alleviated the cell pollution that exists among the prior art and influenced cell survival rate technical problem, reached the technological effect who promotes cell survival rate.
Description
Technical Field
The utility model relates to the technical field of medical experimental equipment, in particular to a test tube constant temperature device, cell recovery equipment and a cell recovery instrument.
Background
Cell cryopreservation is one of the main methods for cell preservation, and the cell is preserved at low temperature by using a cryopreservation technology, so that the cell can be temporarily separated from a growth state and the cell characteristics can be preserved, and the cell is recovered for experiment when needed. Cell recovery refers to the process of cell growth recovery by re-culturing after thawing cells frozen in liquid nitrogen or in a refrigerator at-70 ℃. When the normal temperature state is recovered, the morphological structure of the cell is kept normal, and the biochemical reaction can be recovered. Different from the cell freezing, the temperature rise is fast in the cell recovery process, so that the moisture is prevented from entering the cells in the unfreezing process to form ice crystals, and the survival of the cells is prevented from being influenced.
At present, the traditional cell recovery method is to take out a freezing tube, directly immerse the freezing tube in warm water of 37 ℃ and shake the freezing tube from time to melt the freezing tube as soon as possible. The conventional water temperature recovery method pollutes cells due to unstable state or contact, thereby affecting the survival rate of the cells.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a test tube constant temperature device, cell recovery equipment and a cell recovery instrument, which are used for relieving the technical problem that cell contamination influences the cell survival rate in the prior art.
In order to achieve the above purpose, the embodiment of the present invention adopts the following technical solutions:
in a first aspect, an embodiment of the present invention provides a test tube thermostat device, including: the device comprises two heating modules, two temperature detection modules, an infrared temperature measurement sensor and an attraction electromagnetic valve; a test tube groove for placing a test tube is arranged in the device;
the two heating modules are respectively arranged on two sides of the test tube groove and used for heating the test tubes placed in the test tube groove; the two temperature detection modules are respectively connected with the two heating modules through a control device and used for detecting the temperatures of the two heating modules;
the infrared temperature measuring sensor is used for detecting the temperature of the test tube; the suction electromagnetic valve sucks the test tube after the test tube is placed in the test tube groove and is used for ensuring that the test tube is respectively in fixed contact with the two heating modules.
In some embodiments, the above test tube thermostatic device further comprises: a photoelectric detection module; the photoelectric detection module is used for detecting whether the test tube is inserted into the test tube groove.
In a second aspect, embodiments of the present invention provide a cell recovery apparatus, including: a control device and the test tube thermostat of any one of the above embodiments;
the control device is connected with the test tube constant temperature device;
the test tube constant temperature device is used for sending the collected temperature of the test tube and the temperatures of the two heating modules to the control device;
the control device is used for sending a heating instruction to the test tube constant temperature device according to the received temperature of the test tube and a pre-stored data table;
the test tube constant temperature device is also used for heating the test tube placed in the test tube groove according to the heating instruction. In some embodiments, the control device is a main control chip; the main control chip comprises an AD detection interface, a HEAT interface and a CHECK interface.
In some embodiments, the two temperature detection modules are respectively connected to the control device through the AD detection interfaces;
the two heating modules are respectively connected with the control device through the HEAT interfaces, and the HEAT interfaces are used for controlling the on and off of the triodes;
the two heating modules are respectively connected with the control device through the CHECK interfaces, and the CHECK interfaces are used for detecting heating current and power consumption.
In some embodiments, the test tube thermostatic device comprises a first test tube thermostatic device and a second test tube thermostatic device; the first-class test tube constant temperature device and the second-class test tube constant temperature device are respectively used for heating test tubes with different capacities.
In some embodiments, the apparatus further comprises: an interaction control device; the interactive control device is used for displaying the temperature of the test tube in the test tube constant temperature device and the temperatures of the two heating modules.
In some embodiments, the apparatus further comprises: the device comprises a power input module, a memory circuit and a voice prompt module;
the power input module is used for providing power for the control device;
the memory circuit is used for storing temporary operation data;
the voice prompt module comprises a buzzer and a loudspeaker and is used for giving out a prompt in the cell resuscitation operation process.
In some embodiments, the apparatus further comprises: a voltage detection and protection module; the voltage detection and protection module is used for protecting the stable operation of a system, and when the over-temperature or overload of the equipment is detected, the automatic power-off is carried out for protection.
In a third aspect, an embodiment of the present invention provides a cell resuscitator, including: a housing and a cell resuscitation device as in any one of the embodiments above.
The utility model provides a test tube constant temperature device, cell resuscitation equipment and a cell resuscitation instrument, wherein the device comprises two heating modules, two temperature detection modules, an infrared temperature measurement sensor and an attraction electromagnetic valve; a test tube groove for placing a test tube is arranged in the device; the two heating modules are respectively arranged on two sides of the test tube groove and used for heating the test tube; the two temperature detection modules are respectively used for detecting the temperatures of the two heating modules; the infrared temperature measuring sensor is used for detecting the temperature of the test tube; the actuation solenoid valve actuation after the test tube is put into the test-tube groove for guarantee the test tube respectively with two heating module fixed contact, alleviated the cell pollution that exists among the prior art and influenced cell survival rate technical problem, reached the technological effect who promotes cell survival rate.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
Fig. 1 is a schematic structural diagram of a test tube thermostat provided in an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of a cell recovery apparatus according to an embodiment of the present invention;
FIG. 3 is a schematic structural diagram of another cell resuscitation apparatus provided in an embodiment of the present invention;
fig. 4 is a schematic structural diagram of a cell resuscitator according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Cell cryopreservation is one of the main methods for cell preservation, and the cell is preserved at low temperature by using a cryopreservation technology, so that the cell can be temporarily separated from a growth state and the cell characteristics can be preserved, and the cell is recovered for experiment when needed. Cell recovery refers to the process of cell growth recovery by re-culturing after thawing cells frozen in liquid nitrogen or in a refrigerator at-70 ℃. When the normal temperature state is recovered, the morphological structure of the cell is kept normal, and the biochemical reaction can be recovered. Different from the cell freezing, the temperature rise is fast in the cell recovery process, so that the moisture is prevented from entering the cells in the unfreezing process to form ice crystals, and the survival of the cells is prevented from being influenced.
At present, the traditional cell recovery method is to take out a freezing tube, directly immerse the freezing tube in warm water of 37 ℃ and shake the freezing tube from time to melt the freezing tube as soon as possible. The conventional water temperature recovery method pollutes cells due to unstable state or contact, thereby affecting the survival rate of the cells.
Based on this, the embodiment of the utility model provides a test tube constant temperature device, a cell recovery device and a cell recovery instrument, so as to alleviate the technical problem that cell contamination affects the cell survival rate in the prior art. Some embodiments of the utility model are described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.
In order to facilitate understanding of the present application, a test tube thermostat device provided by an embodiment of the present invention is first described, referring to a schematic structural diagram of a test tube thermostat device shown in fig. 1, where the test tube thermostat device includes: two heating modules (110 and 120), two temperature detection modules (130 and 140), an infrared temperature measurement sensor 150 and a suction electromagnetic valve 160; the device is internally provided with a test tube slot 100 for placing a test tube.
The two heating modules (110 and 120) are respectively arranged at two sides of the test tube slot 100 and used for heating test tubes placed in the test tube slot 100; the two temperature detection modules (130 and 140) are respectively connected with the two heating modules (110 and 120) through a control device and used for detecting the temperatures of the two heating modules.
The infrared temperature sensor 150 is used for detecting the temperature of the test tube placed in the test tube groove; the suction solenoid valve 160 sucks after the test tube is placed in the test tube slot, and is used for ensuring that the test tube is respectively in fixed contact with the two heating modules.
In an embodiment, the above test tube thermostatic device may further include: a photo detection module 170 for detecting whether the cuvette is inserted into the cuvette slot 100.
Because the test tubes for containing cells are different in size, in order to meet the requirements of more application scenes, the test tube thermostat provided by the embodiment of the application is provided with various configurations, and as a specific example, a test tube groove of the first test tube thermostat can be used for containing a large test tube with the capacity of 4.8-5.2 ml; the second tube thermostat has a tube well for holding a small tube with a volume of 1.8-2.0 ml. In addition, the sizes of the heating modules arranged on the two sides of the test tube groove can be correspondingly adjusted along with the size of the test tube groove.
According to the test tube constant temperature device provided by the embodiment of the utility model, the test tube filled with the cells is placed in the constant temperature device capable of heating and monitoring the temperature in real time, so that the stable state of the cells is maintained as much as possible, the cells are prevented from being polluted due to contact, and the survival rate of the cells is improved.
The embodiment of the present invention further provides a cell recovery apparatus, referring to the schematic structural diagram of a cell recovery apparatus shown in fig. 2, the apparatus includes a control device 220 and a test tube thermostat 210 in any one of the above embodiments.
Wherein, controlling means 220 is connected with test tube constant temperature equipment 210, and test tube constant temperature equipment 210 is used for sending the temperature of the test tube of gathering and the temperature of two heating modules to controlling means 220.
The control device 220 is used for receiving the temperature of the test tube and the temperature of the heating module and sending a heating instruction to the test tube constant temperature device 210; the test tube constant temperature device is also used for receiving a heating instruction and heating the test tube placed in the test tube groove.
The control device is a main control chip, and the main control chip comprises an AD detection interface, a HEAT interface and a CHECK interface. As a specific example, the master control chip may be an STM32F405RGT6 type single chip microcomputer.
In one embodiment, the two temperature detection modules are respectively connected with the control device through AD detection interfaces; the two heating modules are respectively connected with the control device through a HEAT interface, and the HEAT interface is used for controlling the on and off of the triode; the two heating modules are respectively connected with the control device through a CHECK interface, and the CHECK interface is used for detecting heating current and power consumption.
When the control device receives the current test tube temperature and the current heating rod temperature, the control device calls the temperature of the heating rod corresponding to the target test tube temperature from a pre-stored data table, generates a heating instruction and sends the heating instruction to the test tube constant temperature device.
Correspondingly, in order to satisfy the requirement of holding cell recovery in different capacity test tubes, the test tube constant temperature equipment that provides in the embodiment of this application includes: a first-class test tube constant temperature device and a second-class test tube constant temperature device; the first-class test tube constant temperature device and the second-class test tube constant temperature device are respectively used for heating test tubes with different capacities.
The test tube groove in the first type of test tube constant temperature device is a first test tube groove, and the test tube groove in the second type of test tube constant temperature device is a second test tube groove; the first test tube groove and the second test tube groove are used for placing test tubes with different capacities respectively. For example, the first test tube well can hold a large test tube with a capacity of 4.8-5.2 ml; the second test tube groove can be used for placing a small test tube with the capacity of 1.8-2.0 ml.
In a preferred mode, referring to fig. 3, the cell resuscitating apparatus provided in this embodiment comprises two first-type cuvette thermostats (210-1) and two second-type cuvette thermostats (210-2), i.e., two large cuvettes and two small cuvettes can be placed. Different types and numbers of test tube thermostats can be assembled, generally according to the needs of the user, for example: a cell recovery device comprises a first type test tube and two second type test tubes and the like.
In order to observe the heating condition of the test tube in the test tube thermostat in real time, in one embodiment, referring to fig. 3, the cell resuscitation apparatus may further include an interactive control device 230, which may be used to display the temperature of the test tube in the test tube thermostat and the temperatures of the two heating modules. As a specific example, the interactive control device may be a 4.3 inch display screen.
In one embodiment, the cell recovery apparatus may further include: a power input module 240, a memory circuit 250, and a voice prompt module 260. The power input module is used for providing power for the control device, can be used for 24V direct current input, and outputs stable 3.3V power to the single chip microcomputer through the DCDC power supply. The memory circuit is used for storing temporary operation data. The voice prompt module can comprise a buzzer and a loudspeaker and is used for sending out a prompt in the cell resuscitation operation process.
Furthermore, the above cell recovery apparatus may further include: and a voltage detection and protection module 270 for protecting the stable operation of the system, and automatically cutting off the power for protection when the over-temperature or overload of the equipment is detected.
The cell resuscitation equipment provided by the embodiment of the utility model comprises a test tube constant temperature device, wherein the device comprises two heating modules, two temperature detection modules, an infrared temperature measurement sensor and an attraction electromagnetic valve; a test tube groove for placing a test tube is arranged in the device; the two heating modules are respectively arranged on two sides of the test tube groove and used for heating the test tube; the two temperature detection modules are respectively used for detecting the temperatures of the two heating modules; the infrared temperature measuring sensor is used for detecting the temperature of the test tube; the suction solenoid valve is sucked after the test tube is placed into the test tube groove and is used for ensuring that the test tube is respectively in fixed contact with the two heating modules. The technical problem that cell contamination influences the cell survival rate in the prior art can be solved, and the technical effect of improving the cell survival rate is achieved; and the effect of meeting different application requirements is realized by configuring the test tube constant temperature devices with different types and quantities.
The embodiment of the present invention further provides a cell recovery apparatus, referring to a schematic structural diagram of a cell recovery apparatus shown in fig. 4, the cell recovery apparatus includes a housing and the cell recovery device described in any one of the above embodiments, wherein a main body of the cell recovery device is disposed inside the housing, and only a display panel of the interactive control device (display screen) is retained to be fixed on a surface of the housing by a buckle or a screw.
The utility model provides a test tube constant temperature device, cell resuscitation equipment and a cell resuscitation instrument, wherein the test tube constant temperature device comprises two heating modules, two temperature detection modules, an infrared temperature measurement sensor and an attraction electromagnetic valve; a test tube groove for placing a test tube is arranged in the device; the two heating modules are respectively arranged on two sides of the test tube groove and used for heating the test tube; the two temperature detection modules are respectively used for detecting the temperatures of the two heating modules; the infrared temperature measuring sensor is used for detecting the temperature of the test tube; the actuation solenoid valve actuation after the test tube is put into the test-tube groove for guarantee the test tube respectively with two heating module fixed contact, alleviated the cell pollution that exists among the prior art and influenced cell survival rate technical problem, reached the technological effect who promotes cell survival rate.
The cell resuscitating device and the test tube thermostat included in the cell resuscitating apparatus provided in the embodiments of the present application have the same implementation principle and technical effects as those of the foregoing embodiments of the method, and for the sake of brief description, corresponding contents in the foregoing embodiments of the test tube thermostat may be referred to where the embodiments are not mentioned, and are not repeated herein.
It should be noted that: like reference numbers and letters refer to like items in the figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships that the products of the present invention are conventionally placed in use, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the device or element to which the description refers must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the utility model has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. A test tube thermostatic device, characterized by comprising: the device comprises two heating modules, two temperature detection modules, an infrared temperature measurement sensor and an attraction electromagnetic valve; a test tube groove for placing a test tube is arranged in the device;
the two heating modules are respectively arranged on two sides of the test tube groove and used for heating the test tubes placed in the test tube groove; the two temperature detection modules are respectively connected with the two heating modules through a control device and used for detecting the temperatures of the two heating modules;
the infrared temperature measuring sensor is used for detecting the temperature of the test tube; the suction electromagnetic valve sucks the test tube after being placed into the test tube groove and is used for ensuring that the test tube is respectively in fixed contact with the two heating modules.
2. The test tube thermostat device according to claim 1, characterized in that it further comprises: a photoelectric detection module; the photoelectric detection module is used for detecting whether the test tube is inserted into the test tube groove.
3. A cell resuscitation device, comprising: a control device and a test tube thermostatic device according to any one of claims 1-2;
the control device is connected with the test tube constant temperature device;
the test tube constant temperature device is used for sending the collected temperature of the test tube and the temperatures of the two heating modules to the control device;
the control device is used for receiving the temperature of the test tube and sending a heating instruction to the test tube constant temperature device;
the test tube constant temperature device is also used for receiving the heating instruction and heating the test tube placed in the test tube groove.
4. The cell recovery apparatus according to claim 3, wherein the control device is a main control chip; the main control chip comprises an AD detection interface, a HEAT interface and a CHECK interface.
5. The cell resuscitation device according to claim 4, wherein the two temperature detection modules are respectively connected to the control device through the AD detection interfaces;
the two heating modules are respectively connected with the control device through the HEAT interfaces, and the HEAT interfaces are used for controlling the on and off of the triodes;
the two heating modules are respectively connected with the control device through the CHECK interfaces, and the CHECK interfaces are used for detecting heating current and power consumption.
6. The cell resuscitating apparatus of claim 5, wherein the cuvette thermostat comprises a first type of cuvette thermostat and a second type of cuvette thermostat; the first-class test tube constant temperature device and the second-class test tube constant temperature device are respectively used for heating test tubes with different capacities.
7. The cell resuscitation device according to claim 6, further comprising: an interaction control device; the interaction control device is used for displaying the temperature of the test tube in the test tube constant temperature device and the temperatures of the two heating modules.
8. The cell resuscitation device according to claim 6, further comprising: the device comprises a power input module, a memory circuit and a voice prompt module;
the power input module is used for providing power for the control device;
the memory circuit is used for storing temporary operation data;
the voice prompt module comprises a buzzer and a loudspeaker and is used for giving out a prompt in the cell resuscitation operation process.
9. The cell resuscitation device according to claim 6, further comprising: a voltage detection and protection module; the voltage detection and protection module is used for protecting the stable operation of a system, and when the over-temperature or overload of the equipment is detected, the automatic power-off is carried out for protection.
10. A cell resuscitator, comprising: a housing and a cell resuscitation device according to any one of claims 3 to 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122255372.7U CN215975765U (en) | 2021-09-16 | 2021-09-16 | Test tube constant temperature equipment, cell recovery equipment and cell recovery instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122255372.7U CN215975765U (en) | 2021-09-16 | 2021-09-16 | Test tube constant temperature equipment, cell recovery equipment and cell recovery instrument |
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| Publication Number | Publication Date |
|---|---|
| CN215975765U true CN215975765U (en) | 2022-03-08 |
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| CN202122255372.7U Active CN215975765U (en) | 2021-09-16 | 2021-09-16 | Test tube constant temperature equipment, cell recovery equipment and cell recovery instrument |
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| CN (1) | CN215975765U (en) |
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