CN215887064U - Cell culture bottle of improvement structure - Google Patents
Cell culture bottle of improvement structure Download PDFInfo
- Publication number
- CN215887064U CN215887064U CN202122465605.6U CN202122465605U CN215887064U CN 215887064 U CN215887064 U CN 215887064U CN 202122465605 U CN202122465605 U CN 202122465605U CN 215887064 U CN215887064 U CN 215887064U
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- Prior art keywords
- bottle
- piston
- inner bottle
- cell culture
- cells
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- Expired - Fee Related
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- 238000004113 cell culture Methods 0.000 title claims abstract description 15
- 230000006872 improvement Effects 0.000 title claims description 4
- 239000003463 adsorbent Substances 0.000 claims abstract description 13
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 38
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 19
- 239000001569 carbon dioxide Substances 0.000 abstract description 19
- 230000004103 aerobic respiration Effects 0.000 abstract description 6
- 230000004071 biological effect Effects 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000008859 change Effects 0.000 abstract description 3
- 239000007789 gas Substances 0.000 description 18
- 239000013589 supplement Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000012606 in vitro cell culture Methods 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
The utility model provides a cell culture bottle with an improved structure, which comprises an outer bottle and an inner bottle, wherein the inner bottle is arranged in the outer bottle, and the inner bottle is in threaded connection with a bottle opening of the outer bottle; an adsorbent is filled between the outer bottle and the inner bottle; the bottle wall of the inner bottle is provided with a plurality of vent holes, and the inner wall of the inner bottle is bonded with a waterproof breathable film; the inner bottle is provided with a bottle mouth, a piston is connected in the bottle mouth in a sliding mode, and the piston seals the inner bottle. The utility model can discharge the carbon dioxide generated by aerobic respiration of the cells in time, maintain the gas proportion in the bottle, easily lead operators to know the change of the gas proportion in the bottle according to the position of the bottle mouth piston and is beneficial to maintaining the biological activity of the cells in the long-time transportation process.
Description
Technical Field
The utility model relates to the field of cell culture devices, in particular to a cell culture bottle with an improved structure.
Background
Changes in the proportion of gas in the surrounding environment can greatly affect the biological activity of the cells. Carbon dioxide produced by cellular autooxygenation changes the proportion of gas in the surrounding environment. If the surrounding environment can not discharge the carbon dioxide generated by oxygenation out of the body, the excessively accumulated carbon dioxide can influence the alkalinity of the culture amino acid in the culture bottle, and further influence the biological activity of cells. Therefore, in vitro cell culture experiments, researchers need to place cell culture flasks in cell incubators with a certain gas concentration ratio. The gas regulating system in the incubator can discharge carbon dioxide generated by aerobic respiration of cells, thereby maintaining the proportion of gas in the closed space unchanged. At present, with the continuous development of domestic cell research, cells need to be transported for a long distance sometimes, and in the long-distance transportation process, a large-volume cell incubator and a large gas steel cylinder connected with the cell incubator cannot be carried, carbon dioxide generated by the cells in the culture bottle cannot be discharged, so that carbon dioxide in the bottle is accumulated, the pH value of a culture medium is changed, and irreversible damage is caused to the cells. However, there is no culture bottle available in the market which can be used for removing carbon dioxide from cells during long-term transportation.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a cell culture bottle with an improved structure, aiming at the defects of the prior art, the cell culture bottle can timely discharge carbon dioxide generated by aerobic respiration of cells, maintain the gas proportion in the bottle, easily enable an operator to know the change of the gas proportion in the bottle according to the position of a bottle opening piston, and be beneficial to maintaining the biological activity of the cells in the long-time transportation process of the cells.
The embodiment of the utility model is realized by the following technical scheme: a cell culture bottle with an improved structure comprises an outer bottle and an inner bottle, wherein the inner bottle is arranged in the outer bottle and is in threaded connection with a bottle opening of the outer bottle; an adsorbent is filled between the outer bottle and the inner bottle; the bottle wall of the inner bottle is provided with a plurality of vent holes, and the inner wall of the inner bottle is bonded with a waterproof breathable film; the inner bottle is provided with a bottle mouth, a piston is connected in the bottle mouth in a sliding mode, and the piston seals the inner bottle.
Furthermore, a piston rod is arranged on the piston, and scales are marked on the piston rod.
Furthermore, one end of the piston rod is fixedly connected with the piston, the other end of the piston rod is fixedly provided with a limiting plate, and the geometric dimension of the limiting plate is larger than that of the bottle mouth.
Further, the vent hole is positioned between the bottom of the inner bottle and the connecting position of the inner bottle and the outer bottle.
The technical scheme of the embodiment of the utility model at least has the following advantages and beneficial effects:
1. the utility model has reasonable design and simple structure, can effectively adsorb carbon dioxide additionally generated by aerobic respiration of cells to the surrounding environment by arranging the inner bottle, the outer bottle and the adsorbent, ensures the gas proportion suitable for the survival of the cells in the inner bottle, and does not pollute the environment in the inner bottle by the adsorbent;
2. according to the utility model, the waterproof breathable film is arranged, so that the culture solution can be isolated, the culture solution is ensured to be always in the inner bottle, carbon dioxide generated by aerobic respiration of cells can be allowed to pass through, and the adsorbent is ensured to be capable of effectively absorbing carbon dioxide additionally generated by the cells;
3. according to the utility model, by arranging the piston, the proportional content of the gas in the inner bottle can be known according to the position of the piston, and an operator can supplement the gas in the bottle according to the position of the piston, so that the gas culture environment of cells is greatly ensured.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural view of the present invention;
icon: 1-outer bottle; 2-an adsorbent; 3-inner bottle; 4-a vent hole; 5-waterproof breathable film; 6-a piston; 7-a piston rod; 8-limiting plate.
Detailed Description
In the description of the present invention, it should be noted that, if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings or the orientation or positional relationship which is usually placed when the products of the present invention are used, it is only for convenience of describing the present invention and simplifying the description, but it is not intended to indicate or imply that the device or element which is referred to must have a specific orientation, be constructed in a specific orientation and be operated, and thus, should not be construed as limiting the present invention.
In the description of the present invention, it should be further noted that unless otherwise explicitly stated or limited, the terms "disposed," "mounted," "connected," and "connected" should be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in fig. 1, the cell culture bottle with an improved structure comprises an outer bottle 1 and an inner bottle 3, wherein an inner thread is arranged at the bottle mouth of the outer bottle 1, and an outer thread is arranged on the outer wall of the inner bottle 3 at the corresponding position; the adsorbent 2 is filled between the outer bottle 1 and the inner bottle 3, the inner bottle 3 is arranged in the outer bottle 1, the inner bottle 3 is in threaded connection with the bottle mouth of the outer bottle 1, the threaded connection needs to be sealed, and the adsorbent 2 is prevented from being influenced by external air; the inner bottle 3 is characterized in that the bottle wall of the inner bottle 3 is provided with a plurality of vent holes 4, the vent holes 4 are used for circulating carbon dioxide additionally generated by cells, the inner wall of the inner bottle 3 is bonded with a waterproof breathable film 5, and the waterproof breathable film 5 can prevent macromolecules such as water molecules and the like from permeating and allow micromolecules such as gas and the like to permeate; interior bottle 3 has the bottleneck, sliding connection has piston 6 in the bottleneck, bottle 3 in the piston 6 is sealed, along with going on of cultivateing, piston 6 can slide to the bottle, and operating personnel can know the gas proportion content in interior bottle 3 according to 6 positions of piston to supplementary gas supplements, and the gaseous culture environment of cell is guaranteed to the at utmost.
In the embodiment, in order to conveniently pull out the piston 6 and avoid the situation that the piston 6 is too close to the inside of the inner bottle 3 and the piston 6 cannot be taken out, the piston 6 is provided with a piston rod 7; in order to more intuitively know the change of the gas proportion in the bottle, volume scales are marked on the piston rod 7.
In this embodiment, in order to prevent the piston rod 7 and the piston 6 from completely entering the inner bottle 3 and causing the piston 6 to be unable to be taken out, one end of the piston rod 7 is fixedly connected with the piston 6, the other end of the piston rod 7 is fixedly provided with a limiting plate 8, the geometric dimension of the limiting plate 8 is larger than that of the bottle mouth, and the limiting plate 8 can limit the piston 6 to continuously move into the inner bottle 3.
In this embodiment, the vent hole 4 is located between the bottom of the inner bottle 3 and the connecting position of the inner bottle 3 and the outer bottle 1, in order to allow the extra carbon dioxide generated by aerobic respiration of cells to be directly absorbed by the adsorbent 2 after passing through the waterproof and breathable membrane 5.
In this embodiment, the cell culture flask with an improved structure works as follows:
when the cells are transported for a long time, the cells in the culture bottle can breathe in an aerobic manner, the cells can release extra carbon dioxide to the surrounding environment after consuming oxygen, the gas proportion in the surrounding environment is changed, the accumulated excessive carbon dioxide can influence the acidity and alkalinity of the culture medium, and the biological activity of the cells is further influenced, in the embodiment, the extra carbon dioxide released by the cells can be absorbed by the adsorbent 2 on the back of the waterproof breathable film 5, and after the adsorbent 2 absorbs the extra carbon dioxide, the proportion of the gas components in the inner bottle 3 is guaranteed to be stable all the time; after carbon dioxide is absorbed by the adsorbent 2, the air pressure in the inner bottle 3 is lower than the external air pressure, the piston 6 moves towards the inner bottle 3 under the action of the external air pressure, the moving distance is the air reduction amount, when the piston 6 moves to the set position, namely the air proportion changes and exceeds the cell threshold value, an operator can pull out the piston 6 through the piston rod 7 to supplement new air into the inner bottle 3, and the biological activity of cells in the long-time transportation process is fully ensured.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (4)
1. A cell culture bottle of improvement structure which characterized in that: the bottle comprises an outer bottle (1) and an inner bottle (3), wherein the inner bottle (3) is arranged in the outer bottle (1), and the inner bottle (3) is in threaded connection with a bottle opening of the outer bottle (1); an adsorbent (2) is filled between the outer bottle (1) and the inner bottle (3); the bottle wall of the inner bottle (3) is provided with a plurality of vent holes (4), and the inner wall of the inner bottle (3) is bonded with a waterproof breathable film (5); the inner bottle (3) is provided with a bottle mouth, a piston (6) is connected in the bottle mouth in a sliding mode, and the piston (6) seals the inner bottle (3).
2. The improved structure of a cell culture flask according to claim 1, wherein: the piston (6) is provided with a piston rod (7), and scales are marked on the piston rod (7).
3. The improved structure of a cell culture flask according to claim 2, wherein: one end of the piston rod (7) is fixedly connected with the piston (6), the other end of the piston rod (7) is fixedly provided with a limiting plate (8), and the geometric dimension of the limiting plate (8) is larger than that of the bottle mouth.
4. The improved structure of a cell culture flask according to claim 1, wherein: the vent hole (4) is positioned between the bottom of the inner bottle (3) and the connecting position of the inner bottle (3) and the outer bottle (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122465605.6U CN215887064U (en) | 2021-10-13 | 2021-10-13 | Cell culture bottle of improvement structure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122465605.6U CN215887064U (en) | 2021-10-13 | 2021-10-13 | Cell culture bottle of improvement structure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215887064U true CN215887064U (en) | 2022-02-22 |
Family
ID=80250428
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122465605.6U Expired - Fee Related CN215887064U (en) | 2021-10-13 | 2021-10-13 | Cell culture bottle of improvement structure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215887064U (en) |
-
2021
- 2021-10-13 CN CN202122465605.6U patent/CN215887064U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220222 |