CN215861127U

CN215861127U - Reagent platform locking mechanism

Info

Publication number: CN215861127U
Application number: CN202121958293.6U
Authority: CN
Inventors: 唐清泰; 李刚; 王雷; 张志强
Original assignee: Beijing Applied Biological Technologies Co ltd
Current assignee: Beijing Applied Biological Technologies Co ltd
Priority date: 2021-08-19
Filing date: 2021-08-19
Publication date: 2022-02-18
Anticipated expiration: 2031-08-19

Abstract

The utility model relates to the technical field of nucleic acid detection, in particular to a reagent table locking mechanism which comprises a hook component and a clamping block component, wherein the hook component comprises a hook seat arranged on a nucleic acid extraction module, a stepped blind hole is formed in the hook seat, a linear bearing is arranged in the stepped blind hole, a hook shaft is arranged in the linear bearing, a shaft shoulder is arranged at one end of the hook shaft, and the shaft shoulder is in contact with the linear bearing and is used for fixing the hook shaft; the other end of the hook shaft is provided with a groove; the fixture block assembly comprises a mounting bracket mounted on the PCR amplification module, a frame electromagnet is arranged on the mounting bracket, and a fixture block is arranged below the frame electromagnet; the clamping block can be clamped in the groove. The reagent transferring device is compact in structure and ingenious in design, not only is reagent transferring automation realized, but also an intermediate transferring platform is omitted, and the influence of the intermediate transferring platform on the reagent is avoided.

Description

Reagent platform locking mechanism

Technical Field

The utility model relates to the technical field of nucleic acid detection, in particular to a reagent table locking mechanism.

Background

Nucleic acid extraction is an essential link in various molecular biology experiments, and almost every laboratory requires nucleic acid extraction for separation and purification of biomolecules. The nucleic acid extractor is an instrument which automatically finishes nucleic acid extraction work by using a matched nucleic acid extraction reagent, and is widely applied to the fields of clinical disease diagnosis, microbiological detection and the like; after nucleic acid extraction, PCR detection, which is a molecular biological technique for amplifying specific DNA fragments, is required. With the development of nucleic acid detection technology, the integrated nucleic acid detection and amplification machine gradually develops towards automation and simplification, but the integrated nucleic acid detection and amplification machine in the market at present is generally complicated in operation process, and is not fully automated in the experimental process, and particularly in the process of adding amplification reagents, because the nucleic acid detection module and the PCR amplification module are required to have no cross contamination, the automation requirement of the process of adding the amplification reagents is particularly strict.

Chinese patent CN110628608A discloses a full-automatic nucleic acid detection device, which comprises a machine body unit, wherein the machine body unit is divided into an upper layer and a lower layer, and the middle is separated by a baffle provided with an upper channel isolation door unit; the sample preparation processing area is positioned on the upper layer of the machine body unit; the buffer zone and the PCR amplification zone are positioned at the lower layer of the machine body unit and are arranged from top to bottom, and the middle of the buffer zone and the PCR amplification zone are separated by a baffle plate provided with a lower channel isolation door unit; and the PCR amplification area is provided with a lifting transfer platform, and the lifting transfer platform is used for transferring the PCR tube. Compact structure, convenient installation, low manufacturing cost, suitability for scale popularization and application in practice and good market application prospect.

In the prior art, the integrated nucleic acid extraction and PCR detection machine has a complex structure of a lifting transfer platform for transferring an amplification reagent tube, and the up-and-down motion process of the lifting transfer platform can cause the influence of shaking and the like on the reagent.

Therefore, it is highly desirable to design a reagent table locking mechanism for locking the nucleic acid extraction module and the PCR amplification module without directly transferring reagents from the nucleic acid extraction module to the PCR amplification module through an intermediate transfer platform.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems in the prior art, the utility model provides a reagent table locking structure, which adopts the following technical scheme:

a reagent station locking mechanism comprising:

the hook assembly comprises a hook seat arranged on the nucleic acid extraction module, a stepped blind hole is formed in the hook seat, a linear bearing is arranged in the stepped blind hole, a hook shaft is arranged in the linear bearing, a shaft shoulder is arranged at one end of the hook shaft, and the shaft shoulder is in contact with the linear bearing and used for fixing the hook shaft; the other end of the hook shaft is provided with a groove;

the fixture block assembly comprises a mounting bracket mounted on the PCR amplification module, a frame electromagnet is arranged on the mounting bracket, and a fixture block is arranged below the frame electromagnet;

the clamping block can be clamped in the groove.

Furthermore, an anti-backlash spring and a baffle plate penetrate through the hook shaft; one end of the anti-backlash spring is in contact with the linear bearing, the other end of the anti-backlash spring is in contact with the blocking piece, and the blocking piece can slide on the hook shaft.

Furthermore, a clamp spring is arranged between the blocking piece and the groove on the hook shaft.

Furthermore, a blind hole is formed in the end face, close to the shaft shoulder, of one side of the hook shaft, a safety spring is arranged in the blind hole, one end of the safety spring is in contact with the bottom face of the blind hole, and the other end of the safety spring is in contact with the hook seat.

Furthermore, the bottom of the stepped blind hole is provided with a through hole, a set screw is arranged in the through hole, and the set screw is in contact with the safety spring.

Furthermore, a chamfer is arranged on the end face of one side, close to the groove, of the hook shaft.

Further, the frame electromagnet is connected with the clamping block through a screw.

The utility model also provides a nucleic acid extraction and PCR amplification integrated machine which comprises the reagent table locking mechanism.

The utility model has the beneficial effects that:

1. according to the utility model, by arranging the hook assembly and the fixture block assembly, when PCR amplification is carried out, the hook assembly moves to be in contact with the PCR amplification module along with the nucleic acid extraction module, the fixture block of the fixture block assembly is clamped into the groove of the hook assembly, the PCR amplification module is connected with the nucleic acid extraction module, and then the PCR amplification module is linked to the outside of the PCR closed cavity along with the nucleic acid extraction module to transfer a reagent; not only realizes the automation of reagent transfer, but also saves an intermediate transfer platform, and avoids the influence of the intermediate transfer platform on the reagent.

2. The utility model has compact structure and smart design, and avoids the impact of the hook shaft on the PCR amplification module by utilizing the buffer action of the anti-backlash spring.

Drawings

Fig. 1 is a schematic structural diagram of a reagent table locking mechanism provided by the utility model.

Fig. 2 is a schematic structural view of the hook assembly.

Fig. 3 is a schematic structural view of the latch assembly.

Description of reference numerals:

1. the hook comprises a hook seat, 2 a linear bearing, 3 a hook shaft, 4 a groove, 5 a mounting bracket, 6 a frame electromagnet, 7 a clamping block, 8 an anti-backlash spring, 9 a blocking piece, 10 a snap spring, 11 a safety spring, 12 and a set screw.

Detailed Description

The technical solutions of the present invention will be described in detail with reference to the accompanying drawings, and it is obvious that the described embodiments are not all embodiments of the present invention, and all other embodiments obtained by those skilled in the art without any inventive work belong to the protection scope of the present invention.

It is to be understood that the terms "center," "upper," "lower," "horizontal," "left," "right," "lateral," "longitudinal," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the present invention and for the simplicity of description, and do not indicate or imply that the referenced devices or elements must be in a particular orientation, constructed and operated in a particular orientation, and thus are not to be considered as limiting the present invention.

As shown in fig. 1 to 3, the present invention provides a reagent table locking mechanism, comprising:

the hook assembly comprises a hook seat 1 installed on the nucleic acid extraction module, a stepped blind hole in the horizontal direction is formed in the hook seat 1, a linear bearing 2 is arranged in the stepped blind hole, a hook shaft 3 is arranged in the linear bearing 2, a shaft shoulder is arranged at one end of the hook shaft 3, and the shaft shoulder is in contact with the linear bearing 2 and is used for fixing the hook shaft 3; the other end of the hook shaft 3 is provided with a groove 4;

the fixture block assembly comprises a mounting bracket 5 mounted on the PCR amplification module, a frame electromagnet 6 is vertically arranged on the mounting bracket 5, and a fixture block 7 is arranged below the frame electromagnet 6;

the clamping block 7 can be clamped into the groove 4.

Furthermore, an anti-backlash spring 8 and a baffle plate 9 penetrate through the hook shaft 3; one end of the anti-backlash spring 8 is in contact with the linear bearing 2, the other end of the anti-backlash spring 8 is in contact with the blocking piece 9, and the blocking piece 9 can slide on the hook shaft 3.

Furthermore, a clamp spring 10 is arranged between the blocking piece 9 and the groove 4 on the hook shaft 3, when the hook assembly is separated from the fixture block assembly, the anti-backlash spring 8 pushes the blocking piece 9 to move outwards, and the clamp spring 10 is used for preventing the blocking piece 9 from being separated from the hook shaft 3.

Further, the hook shaft 3 is close to a blind hole is formed in one side end face of the shaft shoulder, a safety spring 11 is arranged in the blind hole, one end of the safety spring 11 is in contact with the bottom face of the blind hole, and the other end of the safety spring 11 is in contact with the hook seat 1.

Furthermore, a through hole is formed in the bottom of the stepped blind hole, a set screw 12 is arranged in the through hole, and the set screw 12 is in contact with the safety spring 11.

Further, a chamfer is arranged on one side end face, close to the groove 4, of the hook shaft 3.

Further, the frame electromagnet 6 is connected with the fixture block 7 through screws.

The utility model also provides a nucleic acid extraction and PCR amplification integrated machine, which comprises a nucleic acid extraction module, a PCR amplification module and reagent table locking mechanisms arranged at two sides of the nucleic acid extraction module; the nucleic acid extraction module comprises a reagent platform for nucleic acid extraction, a lead screw mechanism for driving the reagent platform to horizontally move and a first guide rail; the PCR amplification module comprises a PCR amplification platform, a power-off electromagnet for fixing the PCR amplification platform, stainless iron arranged on the PCR amplification platform and matched with the power-off electromagnet, and a second guide rail for supporting the PCR amplification platform; and a connecting hole is formed in the PCR amplification platform.

When PCR amplification is carried out, magnetic force is generated after the power-off type electromagnet is powered off, the stainless iron is tightly attached to the power-off type electromagnet under the action of the magnetic force, and the PCR amplification platform is restrained on vertical plates on two sides of the PCR amplification module under the support of the second guide rail; when a reaction tube needs to be installed on the PCR amplification platform, the reagent platform horizontally slides on the first guide rail under the drive of the screw mechanism, when the hook shaft 3 is inserted into the connecting hole of the PCR amplification platform, the anti-backlash spring 8 is compressed under the push of the baffle plate 9, the reagent platform continuously moves forwards, after the hook shaft 3 is inserted into the bottom of the connecting hole of the PCR amplification platform, the frame electromagnet 6 is powered off, the fixture block 7 is clamped into the groove 4, at the moment, the power-off electromagnet is powered on, the reagent platform pulls the PCR amplification platform under the drive of the screw mechanism to be linked together, and the reagent platform pulls the PCR amplification platform out of the PCR amplification chamber to transfer a reagent; the safety spring 11 can ensure that the hook shafts on two sides all contact the bottom of the connecting hole, and is beneficial to improving the hanging success rate.

Although the present invention has been described in detail with reference to examples, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the scope of the present invention.

Claims

1. The utility model provides a reagent platform locking mechanical system, includes couple subassembly and fixture block subassembly, its characterized in that:

the clamping block can be clamped in the groove.

2. The reagent station lock mechanism of claim 1, wherein: the hanger shaft is penetrated with an anti-backlash spring and a baffle plate; one end of the anti-backlash spring is in contact with the linear bearing, the other end of the anti-backlash spring is in contact with the blocking piece, and the blocking piece can slide on the hook shaft.

3. The reagent station lock mechanism of claim 2, wherein: and a clamp spring is arranged between the blocking piece and the groove on the hook shaft.

4. The reagent station lock mechanism of claim 1, wherein: the hook shaft is close to a blind hole is formed in one side end face of the shaft shoulder, a safety spring is arranged in the blind hole, one end of the safety spring is in contact with the bottom face of the blind hole, and the other end of the safety spring is in contact with the hook seat.

5. The reagent station lock mechanism of claim 4, wherein: the bottom of ladder blind hole sets up the through-hole, set screw in the through-hole, set screw with the safety spring contact.

6. The reagent station lock mechanism of claim 1, wherein: and a chamfer is arranged on the end surface of one side of the hook shaft close to the groove.

7. The reagent station lock mechanism of claim 1, wherein: the frame electromagnet is connected with the clamping block through a screw.

8. An integrated nucleic acid extraction and PCR amplification machine comprising the reagent table locking mechanism according to any one of claims 1-7.