CN212255349U - Incubation module - Google Patents

Abstract

The utility model discloses an incubation module, which enables a chip to react at constant temperature within a set temperature, moves freely up and down along the vertical direction and is provided with an incubation groove component, and the incubation groove component comprises a heating plate and a heat preservation plate; a plurality of stacked incubators in the incubator assembly, the plurality of stacked incubators being free to move up and down along the incubator slide. The multiple stacked incubation tanks in the incubation tank assembly have the advantages of large space utilization rate, compact overall structure and small occupied area, and are arranged in the vertical direction, so that the waste of space caused by the tiling and unfolding of equipment is avoided; other components in the time-resolved fluoroimmunoassay analyzer, such as a sample feeding module, a cleaning module and a chip detection module, can be arranged around the incubation module, and the turnover of the chip is carried out by taking the incubation module as a center; the incubation tank component is provided with a heating plate and a heat preservation plate so that the chip can react at a constant temperature within a set temperature.

Description

Incubation module

Technical Field

The utility model belongs to the technical field of medical equipment, especially, relate to an incubation module for time-resolved fluoroimmunoassay appearance.

Background

Microfluidic chips (microfluidic chips) are a hot spot area for the development of current micro Total Analysis Systems (minidesigned Total Analysis Systems). The micro-fluidic chip analysis takes a chip as an operation platform, simultaneously takes analytical chemistry as a basis, takes a micro-electromechanical processing technology as a support, takes a micro-pipeline network as a structural characteristic, takes life science as a main application object at present, and is the key point of the development of the field of the current micro total analysis system. Its goal is to integrate the functions of the entire laboratory, including sampling, dilution, reagent addition, reaction, separation, detection, etc., on a microchip. The micro-fluidic chip is a main platform for realizing the micro-fluidic technology. The device is characterized in that the effective structure (channels, reaction chambers and other functional parts) for containing the fluid is at least in one latitude in micron scale. Due to the micro-scale structure, the fluid exhibits and develops specific properties therein that differ from those of the macro-scale. Thus developing unique assay-generated properties. The characteristics and development advantages of the micro-fluidic chip are as follows: the micro-fluidic chip has the characteristics of controllable liquid flow, extremely less consumption of samples and reagents, ten-fold or hundred-fold improvement of analysis speed and the like, can simultaneously analyze hundreds of samples in a few minutes or even shorter time, and can realize the whole processes of pretreatment and analysis of the samples on line. The application of the micro total analysis system aims to realize the ultimate goal of the micro total analysis system, namely a lab-on-a-chip, and the key application field of the current work development is the field of life science.

Current state of international research: the innovation is mostly focused on the aspects of a separation and detection system; many problems of how to introduce actual sample analysis on a chip, such as sample introduction, sample change, pretreatment, and the like, are still very weak. Its development relies on the development of multidisciplinary crosses.

In chinese patent document CN108414522A, a device for a microfluidic chip and NC membrane multi-flux detection all-in-one machine is disclosed, which comprises a base, wherein a sample introduction module, an incubation module, a cleaning module, an NC membrane detection module and a chip detection module are arranged on the base; the sample injection module is internally provided with a chip sample injection clamping groove and an NC membrane sample injection clamping groove which are mutually independent and are respectively used for inserting a chip or an NC membrane clamping strip; a liquid path and gas path module is also arranged in the sample injection module; the incubation module is provided with a chip incubation groove and an NC membrane incubation groove which are mutually independent, and the sample introduction module, the cleaning module and the NC membrane detection module are arranged along the side surface of the incubation module in the length direction; and conveying the cleaned chip in the cleaning module to the chip detection module for detection.

However, in the technical scheme, each part is arranged along the side surface of the incubation module in the length direction, and is laid and unfolded, so that too much space is occupied, and the equipment is too bulky in the use process; in addition, the connection between the modules needs to be further improved, and the matching is not very smooth. In particular, there is a need for improvements in the structure of incubation modules for use in time-resolved fluoroimmunoassay analyzers; the time-resolved fluoroimmunoassay analyzer is based on the fluoroimmunoassay of a microfluidic chip project.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to provide an incubation module for time-resolved fluoroimmunoassay appearance.

In order to solve the technical problem, the utility model adopts the technical proposal that the incubation module enables the chip to react at constant temperature in a set temperature, the incubation module freely moves up and down along the vertical direction and is provided with an incubation groove component, and the incubation groove component comprises a heating plate and a heat preservation plate; a plurality of stacked incubators in the incubator assembly, the plurality of stacked incubators being free to move up and down along the incubator slide.

The multiple stacked incubation tanks in the incubation tank assembly have the advantages of large space utilization rate, compact overall structure and small occupied area, and are arranged in the vertical direction, so that the waste of space caused by the tiling and unfolding of equipment is avoided; other components in the time-resolved fluoroimmunoassay analyzer, such as a sample feeding module, a cleaning module and a chip detection module, can be arranged around the incubation module, and the turnover of the chip is carried out by taking the incubation module as a center; the incubation tank component is provided with a heating plate and a heat preservation plate so that the chip can react at a constant temperature within a set temperature.

In the specific operation: placing a chip to be detected into an analyzer, and then moving the chip to a liquid path and gas path module under the driving of a chip loading module, so that a sample in a chip sample injection cavity slowly advances along a chip micro-channel and flows to a preset position; the chip is stirred to enable the chip to reach the incubation module, so that the chip reaction cavity performs sample immunoreaction at a preset temperature; after the reaction, stir the chip and wash the module, wash the module and can wash the chip after the reaction, the chip is washd and is accomplished, stirs the chip immediately and arrives the incubation module, sends the chip to the chip detection module by the incubation module and detects in, the incubation module rises to the chip detection module and corresponds the position. The detection result can be displayed on an instrument display screen, and the detection result can be printed by a built-in printer.

The invention is further improved in that the incubation tank assembly is also provided with an incubation tank connecting block; the incubation groove connecting block is connected with an incubation groove moving belt, the band-type brake motor drives the incubation groove moving driving wheel, the incubation groove moving belt drives the incubation groove moving driven wheel to rotate, and therefore the multiple stacked incubation grooves in the incubation groove assembly can move up and down freely along the incubation groove sliding rail.

The invention further improves the structure that the incubation tank slide rail is arranged on an incubation tank slide rail bracket, a buffer block is arranged on the side surface of the incubation tank assembly, and correspondingly, a buffer cushion is arranged at the bottom of the incubation tank slide rail bracket; the incubator is characterized in that a limiting separation blade is arranged on the incubator assembly, a limiting optical coupler connecting block is fixedly connected to the incubator slide rail support, and a limiting optical coupler is mounted on the limiting optical coupler connecting block and used for limiting the up-and-down movement of the incubator.

The side of incubation groove subassembly is provided with the buffer block, and the incubation groove slide rail support bottom that corresponds sets up the blotter, provides the buffering for the incubation groove downstream, prevents the damage, is provided with spacing separation blade on the incubation groove subassembly simultaneously, and incubation groove slide rail support fixedly connected with spacing opto-coupler connecting block, spacing opto-coupler are installed on spacing opto-coupler connecting block for the incubation groove up-and-down motion is spacing.

The invention is further improved in that the incubation groove is provided with a chip pressing strip, and two bulges of the chip pressing strip are matched with the groove of the chip and used for positioning the chip for carrying out incubation reaction.

The invention is further improved in that the heating plate and the heat preservation plate are arranged on the side surfaces of the plurality of stacked incubation tanks.

Compared with the prior art, the utility model discloses technical scheme's beneficial effect is: the incubation modules are arranged in the vertical direction, so that the whole structure is compact and the occupied area is small.

Drawings

The following is a more detailed description of embodiments of the present invention with reference to the accompanying drawings:

FIG. 1 is a schematic structural view of an incubation module of the present invention;

FIG. 2 is an enlarged view of a portion of the cushioning structure of FIG. 1;

FIG. 3 is an enlarged view of a portion of the stop structure of FIG. 1;

FIG. 4 is a schematic diagram of the construction of the incubation well assembly of FIG. 1;

FIG. 5 is a schematic view of the structure of the incubator in FIG. 1;

wherein: 1-an incubation module; 101-an incubation well assembly; 10101-heating plate; 10102-heat preservation sheet; 10103-incubation tank; 102-an incubator slide rail; 103-connecting block of incubation groove; 104-a band-type brake motor; 105-moving the belt in the incubation groove; 106-the incubation groove moving driving wheel; 107-incubation groove motion driven wheel; 108-incubator slide rack; 1081-a cushion pad; 109-a buffer block; 1010-limit baffle plate; 1011-limit optical coupling connecting blocks; 1012-limit optical couplers; 1013-chip layering; 1014-nut.

Detailed Description

The incubation module 1 of the utility model enables the chip to react at a constant temperature within a set temperature; the incubation module 1 freely moves up and down along the vertical direction, as shown in figures 1-5, the incubation module 1 is provided with an incubation tank assembly 101, as shown in figure 2, the incubation tank assembly 101 comprises a heating plate 10101 and a heat preservation plate 10102; as shown in FIG. 2, the plurality of stacked incubators 1013 in the incubator assembly 101 and the plurality of stacked incubators 10103 are free to move up and down along the incubator slide tracks 102. The incubator assembly 101 is provided with a heat plate 10101 and a heat-retaining plate 10102 so that the chip reacts isothermally at a set temperature.

As shown in fig. 1, the incubator assembly 101 is further provided with an incubator connection block 103; the incubator connecting block 103 is connected with an incubator moving belt 105, and the band-type brake motor 104 drives the incubator moving driving wheel 106, and the incubator moving belt 105 drives the incubator moving driven wheel 107 to rotate, so that the plurality of stacked incubators 1013 in the incubator assembly 101 move freely up and down along the incubator slide rail 102.

The incubator slide rail 102 is arranged on an incubator slide rail bracket 108, a buffer block 109 is arranged on the side surface of the incubator assembly 101, and correspondingly, a buffer pad 1081 is arranged at the bottom of the incubator slide rail bracket 108; be provided with spacing separation blade 1010 on the incubator subassembly 101, incubator slide rail support 108 fixedly connected with spacing opto-coupler connecting block 1011, spacing opto-coupler 1012 is installed on spacing opto-coupler connecting block 1011, be used for the spacing of incubator 10103 up-and-down motion.

As shown in FIG. 5, the incubation well 10103 is provided with a chip bead 1012, two protrusions of the chip bead 1012 cooperating with grooves in the chip and being fixed by a nut 1014 for positioning the incubation reaction on the chip.

The heating sheet 10101 and the heat preservation sheet 10102 are disposed at the side of the plurality of stacked incubators 10103.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are intended to illustrate the principles of the invention, and that various changes and modifications may be made without departing from the spirit and scope of the invention, such as some adjustments to the positions of the various components mounted on the base, and such changes and modifications are intended to fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (6)

1. An incubation module for allowing isothermal reaction of a chip within a set temperature, wherein the incubation module is freely movable up and down in a vertical direction, and has an incubation well assembly including a heat plate and a heat-retaining plate; a plurality of stacked incubators in the incubator assembly, the plurality of stacked incubators being free to move up and down along the incubator slide.

2. The incubation module of claim 1, wherein the incubation well assembly is further provided with an incubation well connection block; the incubation groove connecting block is connected with an incubation groove moving belt, the band-type brake motor drives the incubation groove moving driving wheel, the incubation groove moving belt drives the incubation groove moving driven wheel to rotate, and therefore the multiple stacked incubation grooves in the incubation groove assembly can move up and down freely along the incubation groove sliding rail.

3. The incubation module of claim 2, wherein the incubation well slide rails are disposed on incubation well slide rail brackets, the sides of the incubation well assembly are provided with buffer blocks, and correspondingly, a buffer pad is disposed at the bottom of the incubation well slide rail brackets; the incubator is characterized in that a limiting separation blade is arranged on the incubator assembly, a limiting optical coupler connecting block is fixedly connected to the incubator slide rail support, and a limiting optical coupler is mounted on the limiting optical coupler connecting block and used for limiting the up-and-down movement of the incubator.

4. The incubation module of claim 1, wherein the incubation well is provided with a chip bead, two protrusions of which cooperate with grooves of the chip for positioning the chip for performing the incubation reaction.

5. The incubation module of claim 4, wherein the chip molding is secured by a nut.

6. The incubation module of claim 1, wherein the heat and incubation strips are disposed on the sides of a plurality of stacked incubators.

Images