CN215795482U - Bovine tuberculosis gamma-interferon ELISA (enzyme-linked immunosorbent assay) detection kit - Google Patents

Bovine tuberculosis gamma-interferon ELISA (enzyme-linked immunosorbent assay) detection kit Download PDF

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Publication number
CN215795482U
CN215795482U CN202122032943.0U CN202122032943U CN215795482U CN 215795482 U CN215795482 U CN 215795482U CN 202122032943 U CN202122032943 U CN 202122032943U CN 215795482 U CN215795482 U CN 215795482U
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reagent
reaction plate
kit
gamma
placing frame
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CN202122032943.0U
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耿玉静
王善普
张鹏翼
智雪玲
王德迪
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Luoyang Laipson Information Technology Co ltd
Luoyang Modern Biotech Institute Co ltd
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Luoyang Laipson Information Technology Co ltd
Luoyang Modern Biotech Institute Co ltd
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Abstract

The utility model provides a bovine tuberculosis gamma-interferon ELISA detection kit, which comprises a kit body and a reagent placing frame arranged in the kit body, wherein the top of the kit body is provided with a kit cover; the reagent placing frame is positioned at the bottom of the reaction plate placing cavity and is provided with a plurality of reagent slotted holes and reagent tubes clamped in the reagent slotted holes, and the reagent placing frame is provided with reaction plate clamping grooves along the length direction of the reagent placing frame; an antigen coated reaction plate is vertically clamped in the reaction plate clamping groove, and the antigen coated reaction plate can be horizontally and correspondingly clamped into the reaction plate placing cavity; and a power driving assembly for driving the reagent placing rack to lift is arranged at the bottom of the reagent placing rack in the box body. The utility model has reasonable and novel design, compact structure and convenient use, does not need to be additionally provided with other reagents, and can conveniently and accurately detect the sample.

Description

Bovine tuberculosis gamma-interferon ELISA (enzyme-linked immunosorbent assay) detection kit
Technical Field
The utility model relates to the technical field of kits, in particular to an ELISA detection kit for bovine tuberculosis gamma-interferon.
Background
Bovine tuberculosis is a chronic zoonosis caused by mycobacterium bovis. The disease is easy to infect and is epidemic worldwide, is determined as a B-type animal infectious disease by the world animal health organization, and is classified as a second-type animal epidemic disease in China. There are 3 species of mycobacteria that cause tuberculosis in humans and animals: human type Mycobacterium tuberculosis, bovine type Mycobacterium tuberculosis, and avian type Mycobacterium tuberculosis.
The key to control bovine tuberculosis and human tuberculosis lies in the detection of bovine tuberculosis bacteria. Currently, the accepted method by the world's animal health organization is bovine tuberculin intradermal allergy (TST). However, the TST detection procedure is complicated, time-consuming and labor-consuming, the result judgment subjectivity is strong, and the operation of a professional is required, so that the implementation effect of the quarantine-culling policy is greatly reduced, and the TST detection method can only be used for a living test, but cannot be used for retrospective analysis of stored samples, and is not suitable for the general investigation of the bovine tuberculosis of wild animals and remote mountain areas.
Therefore, it is necessary to provide a bovine tuberculosis gamma-interferon ELISA kit to solve the existing problems.
Disclosure of Invention
In view of the above, the utility model provides an ELISA detection kit for bovine tuberculosis gamma-interferon, which has a compact structure and is convenient to use, does not need to be additionally provided with other reagents, and can be used for conveniently and accurately detecting a sample.
In order to solve the technical problems, the utility model provides an ELISA (enzyme-linked immunosorbent assay) kit for bovine tuberculosis gamma-interferon, which comprises a kit body and a reagent placing rack arranged in the kit body, wherein a box cover is arranged at the top of the kit body;
the reagent placing frame is positioned at the bottom of the reaction plate placing cavity and is provided with a plurality of reagent slotted holes and reagent tubes clamped in the reagent slotted holes, and the reagent placing frame is provided with reaction plate clamping grooves along the length direction of the reagent placing frame;
an antigen coated reaction plate is vertically clamped in the reaction plate clamping groove, and the antigen coated reaction plate can be horizontally and correspondingly clamped into the reaction plate placing cavity;
and a power driving assembly for driving the reagent placing rack to lift is arranged at the bottom of the reagent placing rack in the box body.
Further, the reagent slots include assay reagent slots, diluent slots, and detergent slots; a measuring reagent tube, a diluting reagent tube and a washing reagent tube are clamped and arranged corresponding to the measuring reagent slot hole, the diluting reagent slot hole and the washing reagent slot hole.
Furthermore, a placing groove is formed in the surface of one end of the antigen-coated reaction plate, and detection test paper is arranged in the placing groove;
the other end of the antigen coating reaction plate is provided with a first reaction area and a second reaction area, and the first reaction area and the second reaction area are provided with a plurality of reaction tanks.
Furthermore, the reagent placing frame is positioned around the reaction plate placing cavity and is provided with a plurality of L-shaped supporting blocks for supporting the antigen coated reaction plate.
Furthermore, a guide slot hole is formed in the center of the upper end face of the reagent placing frame along the height direction, a power transmission cavity is formed in the center of the bottom face of the reagent placing frame and corresponds to the guide slot hole, and a transmission shaft groove is formed in the middle of the bottom face of the reagent placing frame and communicated with one end of the power transmission cavity;
the power driving assembly comprises a transmission screw rod which is rotatably arranged in a guide groove hole, and the bottom of the guide groove hole is matched with the transmission screw rod to be provided with a screw rod sleeve;
the transmission screw rod lower part cover is equipped with the second bevel gear, is provided with first bevel gear with the cooperation of second bevel gear, and first bevel gear is fixed to be set up in power transmission axle one end, and the power transmission axle other end wear out and with power capstan winch fixed connection from box body one side, set up the capstan winch groove that supplies the power capstan winch to install on the box body lateral wall.
Furthermore, a plurality of positioning clamping grooves are formed in the four side faces of the reagent placing rack along the height direction;
the positioning clamping strips are correspondingly arranged on the periphery of the inner side of the box body and correspond to the positioning clamping grooves, and the reagent placing frame slides up and down along the positioning clamping strips in the box body.
Furthermore, the box cover is buckled on the box body through a hinged pivot;
one side of the box body opening is provided with a permanent magnet, and one side of the box cover opening is provided with an attraction iron sheet corresponding to the permanent magnet.
Furthermore, a plurality of supporting springs are arranged on the inner side of the box cover along the periphery, and the lower ends of the supporting springs are connected with shockproof pressing plates corresponding to the upper end of the reagent placing frame.
Furthermore, positioning counter bores are formed in four corners of the bottom surface of the reagent placing frame, and positioning rods are accommodated in the positioning counter bores; anti-seismic springs sleeved on the positioning rods are arranged at four corners of the bottom of the box body.
The technical scheme of the utility model at least comprises the following technical effects:
1. the kit has compact structure, and can establish an ELISA detection diagnosis technology which is rapid, accurate, strong in specificity, high in sensitivity and good in repeatability; the use is convenient, other reagents are not required to be additionally prepared, the result judgment is visual, accurate, convenient, reliable and low in cost;
2. the reaction plate is coated with the antigen in a partitioned mode, so that the reaction plate is not only suitable for detecting the bovine tubercle bacillus, but also suitable for detecting other types of strains, and epidemic disease investigation and detection of various animals are met; the reaction structure not only saves space, but also can conveniently detect samples;
3. according to the power driving assembly, the power winch is hinged with the handle, the handle can be buckled in the power winch, the power winch can be manually rotated to transmit power to the first bevel gear and the second bevel gear which are vertically arranged through the power transmission shaft, so that the transmission screw rod can be driven to rotate, the reagent placing rack is driven to lift and move through the rotation of the transmission screw rod, and reagent taking and detection of the reagent placing rack can be conveniently realized;
4. the reagent placing frame structure can stably slide with the box body, so that the reagent placing frame can stably lift along the box body under the action of the power driving assembly;
5. the kit can effectively protect the reagent placing rack in the storage and transportation processes of the kit, and prevent various reagent tubes from being damaged.
Drawings
FIG. 1 is a front view of the internal structure of a reagent cartridge according to an embodiment of the present invention;
FIG. 2 is an end view of the internal structure of the cartridge in the embodiment of the present invention;
FIG. 3 is a schematic view of a reagent rack according to an embodiment of the present invention from a first perspective;
FIG. 4 is a schematic diagram of a reagent rack according to an embodiment of the present invention from a second perspective;
FIG. 5 is a schematic diagram of an antigen-coated reaction plate according to an embodiment of the present invention;
FIG. 6 is a schematic diagram of a reagent rack, a reaction plate, and reagent tubes according to an embodiment of the present invention.
In the figure:
100. a kit; 1. a box cover; 2. a box body; 3. Placing a reagent rack; 301. positioning the clamping groove; 302. positioning the counter bore; 303. positioning a rod; 304. a reaction plate placing cavity; 305. determining reagent slots; 306. a reaction plate slot; 307. a diluent slot; 308. detergent slot holes; 309. a guide slot hole; 310. an elastic support bar; 311. an L-shaped support block; 312. a transmission shaft slot; 313. a power transmission cavity; 4. a support spring; 5. a shockproof pressing plate; 6. positioning the clamping strip; 7. a winch groove; 8. a power winch; 9. a power transmission shaft; 10. a first bevel gear; 11. supporting a frustum; 12. a second bevel gear; 13. an anti-seismic spring; 14. a transmission screw rod; 15. closing the end cap; 16. a hinge pivot; 17. attracting the iron sheet; 18. a permanent magnet; 19. antigen coating reaction plate; 191. a placing groove; 192. a first reaction zone; 193. a second reaction zone; 20. detecting test paper; 21. a assay reagent tube; 22. a diluting reagent tube; 23. and washing the reagent tube.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to fig. 1 to 6 of the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the utility model, are within the scope of the utility model.
As described in fig. 1-6: the bovine tuberculosis gamma-interferon ELISA detection kit 100 comprises a kit body 2 and a reagent placing frame 3 arranged in the kit body 2, wherein a kit cover 1 is arranged at the top of the kit body 2; a reaction plate placing cavity 304 is formed in the upper end face of the reagent placing frame 3, and a plurality of L-shaped supporting blocks 311 for supporting the antigen coated reaction plate 19 are arranged around the reaction plate placing cavity 304 of the reagent placing frame 3; the reagent placing frame 3 is provided with a plurality of reagent slots and reagent tubes clamped in the reagent slots at the bottom of the reaction plate placing cavity 304, the reagent placing frame 3 is provided with a reaction plate clamping groove 306 along the length direction thereof, and elastic support bars 310 for supporting the antigen coated reaction plate are arranged around the reaction plate clamping groove; an antigen-coated reaction plate 19 is vertically clamped in the reaction plate clamping groove 306, and the antigen-coated reaction plate 19 can be horizontally and correspondingly clamped in the reaction plate placing cavity 304; and a power driving assembly for driving the reagent placing frame to lift is arranged at the bottom of the reagent placing frame 3 in the box body 2.
Specifically, the kit disclosed by the utility model is compact in structure, and can establish an ELISA detection diagnosis technology which is rapid, accurate, strong in specificity, high in sensitivity and good in repeatability; the use is convenient, other reagents are not required to be additionally prepared, the sample is not required to be subjected to sterile treatment, the result judgment is visual, accurate and reliable, and the cost is low.
Specifically, as shown in FIG. 3, the reagent wells include an assay reagent well 305, a diluent well 307, and a detergent well 308; a measuring reagent tube 21, a diluting reagent tube 22 and a washing reagent tube 23 are clamped corresponding to the measuring reagent slot 305, the diluting reagent slot 307 and the washing reagent slot 308, wherein the measuring reagent comprises a plurality of reagents aiming at bovine tuberculosis germs, such as a tuberculosis antibody positive control reagent, a tuberculosis negative control reagent, an enzyme-labeled conjugate, an enzyme substrate and other reagents, and details are not repeated herein.
Specifically, as shown in fig. 2-6, a placing groove 191 is formed in one end of the antigen coated reaction plate 19, and a detection test paper 20 is disposed in the placing groove 191 and used for detecting a sample and displaying a detection result; the antigen coating reaction plate 19 is provided with a first reaction area 192 and a second reaction area 193 on the other end plate surface, the first reaction area and the second reaction area are provided with a plurality of reaction grooves, and the antigen coating reaction plate arranged in a partitioning mode is adopted, so that the antigen coating reaction plate is not only suitable for detecting bovine tubercle bacillus, but also suitable for detecting other types of strains, and meets epidemic disease investigation and detection of various animals.
In one embodiment of the present invention, as shown in fig. 3 and 4, the center of the upper end surface of the reagent holding rack 3 is provided with a guiding slot 309 along the height direction, and the center of the bottom surface of the reagent holding rack 3 is provided with a power transmission cavity 313 corresponding to the guiding slot 309, and a transmission shaft groove 312 is arranged in communication with one end of the power transmission cavity 313; the power driving assembly comprises a transmission screw rod 14 which is rotatably arranged in a guide groove hole 309, the upper end of the transmission screw rod 14 is provided with a closed end cover 15, the lower end of the transmission screw rod 14 is provided with a supporting frustum 11, and the bottom of the guide groove hole 309 is matched with the transmission screw rod to be provided with a screw rod sleeve; the utility model discloses a reagent storage rack, including transmission lead screw, transmission lead screw lower part cover and power capstan winch, transmission lead screw lower part cover is equipped with second bevel gear 12, is provided with first bevel gear 10 with second bevel gear 12 cooperation, and first bevel gear 10 is fixed to be set up in power transmission axle 9 one end, and the power transmission axle 9 other end is worn out and with power capstan winch 8 fixed connection from 2 one side of box body, set up the capstan winch groove 7 that supplies the power capstan winch installation on the 2 lateral walls of box body, the last hinge of power capstan winch is connected with the handle, and the handle lock can be in the power capstan winch, and manual rotation power capstan winch can pass through power transmission axle transmission to the perpendicular first bevel gear, second bevel gear who sets up to can drive the transmission lead screw rotation, rotate through the transmission lead screw and drive the reagent rack and carry out the operation of going up and down, realization reagent rack reagent that can be convenient takes and detect.
Based on the above embodiment, in another embodiment of the present embodiment, as shown in fig. 1 and fig. 3-4, a plurality of positioning card slots 301 are formed on four side surfaces of the reagent rack 3 along the height direction; the inside of the box body 2 is provided with positioning clamp strips 6 around corresponding to the positioning clamp grooves, and the reagent placing frame 3 slides up and down along the positioning clamp strips in the box body. In this embodiment, reagent rack structure can form stable slip with the box body to make reagent rack steadily go up and down in the box body under the power drive subassembly effect.
In another embodiment of the present invention, the box cover 1 is fastened to the box body 2 by a hinge pivot 16; one side of the opening of the box body 2 is provided with a permanent magnet 18, and one side of the opening of the box cover 1 is provided with an attraction iron sheet 17 corresponding to the permanent magnet. In this embodiment, the kit can be convenient open and shut to convenient taking of carrying out reagent or detecting the sample.
In another embodiment of the present invention, as shown in fig. 1-2, a plurality of supporting springs 4 are arranged along the periphery inside the box cover 1, and the lower ends of the supporting springs 4 are connected with a shockproof pressing plate 5 corresponding to the upper end of the reagent placing rack 3; positioning counter bores 302 are formed in four corners of the bottom surface of the reagent placing frame 3, and positioning rods 303 are accommodated in the positioning counter bores; anti-seismic springs 13 sleeved on the positioning rods are arranged at four corners of the bottom of the box body 2. In this embodiment, adopt the kit of this kind of setting, can make the kit form the effectual protection of formation to the reagent rack in storage, transportation, prevent the damage of all kinds of reagent pipes.
The use method or the working principle of the utility model are as follows:
the kit has compact structure, and can establish an ELISA detection diagnosis technology which is rapid, accurate, strong in specificity, high in sensitivity and good in repeatability; adopt the reagent supporter structure that the integrated form set up, the reaction plate is in the storage and uses the different states of processing to the efficient realizes reagent supporter space utilization, and it is convenient to use, need not to join in marriage other reagents in addition, and the result is judged image, convenient, accurate, reliable, and is with low costs.
In the present invention, unless otherwise explicitly specified or limited, for example, it may be fixedly attached, detachably attached, or integrated; can be mechanically or electrically connected; the terms may be directly connected or indirectly connected through an intermediate, and may be communication between two elements or interaction relationship between two elements, unless otherwise specifically limited, and the specific meaning of the terms in the present invention will be understood by those skilled in the art according to specific situations.
The foregoing is a preferred embodiment of the present invention, and it should be noted that it would be apparent to those skilled in the art that various modifications and enhancements can be made without departing from the principles of the utility model, and such modifications and enhancements are also considered to be within the scope of the utility model.

Claims (9)

1. A bovine tuberculosis gamma-interferon ELISA detection kit is characterized in that: the kit (100) comprises a kit body (2) and a reagent placing rack (3) arranged in the kit body (2), wherein a kit cover (1) is arranged at the top of the kit body (2);
a reaction plate placing cavity (304) is formed in the upper end face of the reagent placing frame (3), a plurality of reagent slots and reagent tubes clamped in the reagent slots are formed in the bottom of the reaction plate placing cavity (304) of the reagent placing frame (3), and a reaction plate clamping groove (306) is formed in the reagent placing frame (3) along the length direction of the reagent placing frame;
an antigen coated reaction plate (19) is vertically clamped in the reaction plate clamping groove (306), and the antigen coated reaction plate (19) can be horizontally and correspondingly clamped into the reaction plate placing cavity (304);
the power driving assembly used for driving the reagent placing rack to ascend and descend is arranged at the bottom of the reagent placing rack (3) in the box body (2).
2. The bovine tuberculosis gamma-interferon ELISA detection kit of claim 1, wherein: the reagent slots include an assay reagent slot (305), a diluent slot (307), and a detergent slot (308); a measurement reagent tube (21), a dilution reagent tube (22), and a washing reagent tube (23) are fitted in correspondence with the measurement reagent slot (305), the diluent slot (307), and the washing reagent slot (308).
3. The bovine tuberculosis gamma-interferon ELISA detection kit of claim 1, wherein: a placing groove (191) is formed in the surface of one end of the antigen coating reaction plate (19), and detection test paper (20) is arranged in the placing groove (191);
the other end of the antigen coating reaction plate (19) is provided with a first reaction area (192) and a second reaction area (193), and the first reaction area and the second reaction area are provided with a plurality of reaction grooves.
4. The bovine tuberculosis gamma-interferon ELISA detection kit of claim 3, wherein: the reagent placing frame (3) is positioned around the reaction plate placing cavity (304) and is provided with a plurality of L-shaped supporting blocks (311) for supporting the antigen coated reaction plate (19).
5. The bovine tuberculosis gamma-interferon ELISA detection kit of claim 1, wherein: a guide slot hole (309) is formed in the center of the upper end face of the reagent placing frame (3) along the height direction, a power transmission cavity (313) is formed in the center of the bottom face of the reagent placing frame (3) and corresponds to the guide slot hole (309), and a transmission shaft groove (312) is formed in the position, communicated with one end of the power transmission cavity (313);
the power driving assembly comprises a transmission screw rod (14) rotatably arranged in a guide slot hole (309), and the bottom of the guide slot hole (309) is matched with the transmission screw rod to be provided with a screw rod sleeve;
the transmission lead screw lower part cover is equipped with second bevel gear (12), is provided with first bevel gear (10) with second bevel gear (12) cooperation, and first bevel gear (10) are fixed to be set up in power transmission axle (9) one end, and the power transmission axle (9) other end from box body (2) one side wear out and with power capstan winch (8) fixed connection, set up capstan winch groove (7) that supply the power capstan winch to install on box body (2) lateral wall.
6. The bovine tuberculosis gamma-interferon ELISA detection kit of claim 5, wherein: a plurality of positioning clamping grooves (301) are formed in the four side faces of the reagent placing rack (3) along the height direction;
the positioning clamp strips (6) are arranged on the periphery of the inner side of the box body (2) and correspond to the positioning clamp grooves, and the reagent placing frame (3) slides up and down along the positioning clamp strips in the box body.
7. The bovine tuberculosis gamma-interferon ELISA detection kit of any one of claims 1-6, wherein: the box cover (1) is buckled on the box body (2) through a hinged pivot (16);
one side of the opening of the box body (2) is provided with a permanent magnet (18), and one side of the opening of the box cover (1) is provided with an attraction iron sheet (17) corresponding to the permanent magnet.
8. The bovine tuberculosis gamma-interferon ELISA detection kit of claim 7, wherein: a plurality of supporting springs (4) are arranged on the inner side of the box cover (1) along the periphery, and the lower ends of the supporting springs (4) are connected with shockproof pressing plates (5) corresponding to the upper ends of the reagent placing frames (3).
9. The bovine tuberculosis gamma-interferon ELISA detection kit of claim 8, wherein: positioning counter bores (302) are formed in four corners of the bottom surface of the reagent placing frame (3), and positioning rods (303) are accommodated in the positioning counter bores; anti-seismic springs (13) sleeved on the positioning rods are arranged at four corners of the bottom of the box body (2).
CN202122032943.0U 2021-08-27 2021-08-27 Bovine tuberculosis gamma-interferon ELISA (enzyme-linked immunosorbent assay) detection kit Active CN215795482U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202122032943.0U CN215795482U (en) 2021-08-27 2021-08-27 Bovine tuberculosis gamma-interferon ELISA (enzyme-linked immunosorbent assay) detection kit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202122032943.0U CN215795482U (en) 2021-08-27 2021-08-27 Bovine tuberculosis gamma-interferon ELISA (enzyme-linked immunosorbent assay) detection kit

Publications (1)

Publication Number Publication Date
CN215795482U true CN215795482U (en) 2022-02-11

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CN202122032943.0U Active CN215795482U (en) 2021-08-27 2021-08-27 Bovine tuberculosis gamma-interferon ELISA (enzyme-linked immunosorbent assay) detection kit

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