CN215727715U - Horizontal synchronous fast-detection lateral flow chromatography test paper array device - Google Patents

Abstract

The utility model discloses a horizontal synchronous fast-detection lateral flow chromatography test paper array device which comprises two parts, wherein a test paper array plate A is matched with a sample liquid box B with proper width and bearing, the test paper array plate A is provided with a plurality of stable channels, and the sample liquid box B is provided with a certain gradient and a baffle. The lateral flow chromatography test paper array device provides a convenient tool for the array combination of a plurality of test papers and the realization of synchronous rapid detection, avoids the mutual interference of multi-strip detection on a single test paper, can realize the simultaneous analysis of multiple targets by one-time sample loading, and obviously saves the sample loading time, analysis consumables and human resources. The device is small and portable, the operation is simple, and the colorimetric result is immediately available. The process is simple, the cost is low, and the packaging is carried out in the form of light-resistant plastic package and the like, so that the commercialization is easy to realize.

Description

Horizontal synchronous fast-detection lateral flow chromatography test paper array device

Technical Field

The utility model belongs to the technical field of multi-connected test paper detection devices, and particularly relates to a horizontal synchronous fast detection lateral flow chromatography test paper array device.

Background

The lateral flow chromatography test paper as a novel rapid detection sensing technology has the characteristics of simple and convenient operation. The existing commercial test paper is of a single-analyte type, and the synchronous analysis of multiple analytes in a single sample loading is difficult to realize. In the method, a plurality of analytes are actually detected in sequence, real 'synchronous' detection is not realized, and the detection result of the disc test paper with a plurality of analytes is obviously influenced by the distribution uniformity of a fluid.

The defect that a plurality of detection lines interfere with each other exists when the number of the detection lines is increased, and the validity of a plurality of paths cannot be guaranteed by only one quality control line, so that false positives or result deviation can be caused.

The method has important innovative significance and achievement extension significance for synchronous and rapid detection of multiple targets in a complex matrix, development of a high-sensitivity array test paper device which is practical, portable and low in cost, can truly realize time synchronization and does not influence each other.

SUMMERY OF THE UTILITY MODEL

Utility model purpose: in order to overcome the defects in the prior art, the utility model provides a horizontal synchronous rapid detection lateral flow chromatography test paper array device for synchronously and rapidly detecting multiple targets in a complex matrix.

The technical scheme is as follows: in order to achieve the purpose, the utility model adopts the technical scheme that:

a horizontal synchronous fast-detection lateral flow chromatography test paper array device comprises a test paper array plate and a sample solution box;

the test paper array plate is provided with at least one stable channel along the length direction, the two ends of the stable channel are respectively the front end of the stable channel and the tail end of the stable channel, the front end of the stable channel is closed, and the tail end of the stable channel is opened and used for inserting a test paper strip, wherein a water absorption pad of the test paper strip is inserted and fixed at the front end of the stable channel, and a sample pad of the test paper strip is hung outside the stable channel;

the periphery of the sample liquid box is closed, the upper part of the sample liquid box is provided with an opening, a ramp from high to low is arranged in the sample liquid box along the length direction, and the bottom of the ramp is used for storing sample liquid;

when the test paper array device is in a test working state, the water absorption pad of the test paper is fixed at the front end of the stable channel, the test paper array plate is moved, and the sample pad of the test paper slides downwards along the ramp and is correspondingly inserted into the sample liquid at the bottom of the sample liquid box.

Optionally, a stopper in the shape of a rectangular parallelepiped is disposed at an upper portion of the front end of the stabilizing channel, and a lower surface of the stopper is lower than an upper plane of the stabilizing channel.

Optionally, the sum of the height of the stopper and the height of the test strip is not less than the height of the stabilizing channel.

Optionally, the width of each stable channel is 1.1 to 1.3 times the width of the test strip, and the height of each stable channel is 1.2 to 1.5 times the height of the test strip.

Optionally, when the number of the stabilizing channels is at least two, the stabilizing channels are arranged in parallel at equal intervals.

Optionally, the test strip includes a water absorption pad, a chromatographic membrane, a binding pad and a sample pad, the length of the stable channel of the array plate is greater than the length of the water absorption pad and not greater than the total length of the water absorption pad, the chromatographic membrane and the binding pad, and the length of the ramp of the sample box is not less than the length of the sample pad.

Optionally, the length of array board equals the total length of absorbent pad, chromatographic carrier and the combination pad, the upper surface of firm channel has sealed transparent film, and right array board carries out vacuum plastic envelope.

Optionally, a baffle is arranged above the ramp, and the baffle is a strip-shaped plate horizontally arranged along the width direction.

Optionally, the baffle is located above the sample solution and the test strip.

Optionally, the gradient θ of the ramp is 10 ° to 45 °.

Has the advantages that: compared with the prior art, the horizontal synchronous fast detection lateral flow chromatography test paper array device provided by the utility model has the following advantages: the detection line and the corresponding quality control line of each test paper are reserved, real synchronous analysis of multiple test paper multi-target objects can be realized by one-time sample loading, the result deviation caused by mutual interference on a single test paper is avoided, the detection efficiency is improved to a certain extent, and the accuracy of the detection result is guaranteed.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the principles of the utility model and not to limit the utility model. In the drawings:

FIG. 1 is a perspective view of the detecting device of the present invention, (a) is a test strip array plate of the device,

(b) is a sample fluid cartridge of the device;

FIG. 2 is a three-dimensional view of an apparatus according to an embodiment of the present invention, wherein (i) is a front view of a test paper array plate, (ii) is a front view of a sample solution cartridge, (iii) is a top view of a test paper array plate, (iv) is a top view of a sample solution cartridge, (iv) is a left view of a test paper array plate, and (iv) is a left view of a sample solution cartridge;

FIG. 3 is a schematic top view of a lateral flow test strip;

FIG. 4 is a schematic top view of a lateral flow test strip inserted into a channel of a test strip array plate;

FIG. 5 is an enlarged view of a portion of the stop;

FIG. 6 is an assembled view of the strip inserted into the cartridge;

in the figure, a test paper array plate-100, a stable channel-101, a stable channel front end-1011, a stable channel tail end-1012, a block-102, a sample liquid box-200, a ramp-201, a baffle-202, a test paper strip-300, a water absorption pad-301, a chromatographic membrane-302, a binding pad-303 and a sample pad-304.

Detailed Description

The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings, and it should be understood that the preferred embodiments described herein are merely for purposes of illustration and explanation, and are not intended to limit the present invention.

As shown in fig. 1, the horizontal synchronous fast detection lateral flow chromatography test paper array device is composed of two parts, including a test paper array plate 100 and a sample solution box 200. As shown in the three views of the test strip array plate 100 and the sample box 200 shown in fig. 2, the test strip array plate 100 has at least one stable channel 101 along the length direction, the two ends of the stable channel 101 are respectively a stable channel front end 1011 and a stable channel end 1012, the stable channel front end 1011 is closed, the stable channel end 1012 is open for inserting the test strip 300, wherein, as shown in the schematic top view of the lateral flow chromatography test strip shown in fig. 3, the absorbent pad 301 of the test strip 300 is inserted into and fixed at the stable channel front end 1011, and the sample pad 304 of the test strip 300 is suspended outside the stable channel 101; the sample liquid box 200 is closed at the periphery and is opened at the upper part, a ramp 201 from high to low is arranged in the sample liquid box, and the bottom of the ramp 201 is used for storing sample liquid.

As shown in fig. 6, when the test strip is inserted into the sample solution box in the assembled view, when the test strip array device is in the test operation state, the absorbent pad 301 of the test strip 300 is fixed at the front end 1011 of the fixing channel, and the sample pad 304 of the test strip 300 slides down the ramp 201 to be inserted into the sample solution at the bottom of the sample solution box 200.

As an alternative embodiment, as shown in figure 5, the front end 1011 of the stability channel has a stop 102 in the form of a rectangular parallelepiped at an upper position, the lower surface of the stop 102 being lower than the upper plane of the stability channel 101.

In order to achieve easy insertion and fixation of the test strip 300, the sum of the height of the stopper 102 and the height of the test strip 300 (i.e., the thickness of the test strip 300) is not less than the height of the stability channel 101. The stopper 102 is a cuboid structure, sinks a cuboid with the same width as the channel at the top of the stable channel 101, and in one example, when the height of the channel is 2mm, the height of the stopper 102 is designed to be 0.5mm and 3mm long, so that the space reserved below the stopper 102 is slightly smaller than the thickness of the test strip 300, and the test strip 300 can be stably clamped.

As an optional embodiment, the width of each stable channel 101 is 1.1 to 1.3 times the width of the test strip 300, and the height of each stable channel 101 is 1.2 to 1.5 times the height of the test strip 300. In the embodiment of the present application, the width and the height of the stable channel 101 are both designed to be slightly larger than the width and the thickness of the test strip, respectively, and in one example, according to the design that the current commercially available test strip is usually 3mm wide, the width of the channel is designed to be +0.5mm of the test strip width, that is, 3.5mm of the test strip width, so as to facilitate insertion and facilitate fixation; similarly, according to the current commercially available test paper strips, the thickness of the test paper strip is usually 1.5mm, and the height of the channel is designed to be 0.5mm higher than the thickness of the test paper strip, namely 2mm higher than the thickness of the test paper strip, and 0.5mm higher than the thickness of most test paper strips, namely 1.5 mm. In other embodiments, corresponding adjustment can be performed according to the width and thickness actual size of the test strip, so as to obtain the design of the width and height of the specific stable channel 101, so as to meet the requirement that the test strip is convenient to insert and is favorable for fixation, and the embodiment of the application is not limited.

As an alternative embodiment, when there are at least two stabilizing channels 101, the stabilizing channels 101 are parallel and equally spaced. The width of the stability channel 101 is specifically set according to the width of the test strip 300, and in one example, the width of the stability channel 101 can be set to be equidistant for cost savings or when the test strip widths are the same or close. In other examples, also can not equidistance, specifically see service environment's demand, if need put into the multiunit many test paper into more than two kinds of sample liquid systems when carrying out synchronous analysis, can consider to set up the channel of equidistance, corresponding sample liquid box 200 also need correspond simultaneously and carry out the partition region to satisfy in the sample liquid that the test paper strip all effectively corresponds and insert sample liquid box 200 bottom.

As an alternative embodiment, as shown in fig. 3, the test strip 300 includes a water-absorbing pad 301, a chromatographic membrane 302, a binding pad 303 and a sample pad 304, wherein the chromatographic membrane 302 has the following characteristics: the capacity of adsorbing protein (antigen or antibody) is strong and stable; the method has good chromatographic kinetic properties, and the detection time is not too long; good mechanical strength, and is not easy to break and wrinkle; the sensitivity of the experiment is high, and the lower limit of detection can meet the technical requirements; the background is kept clean after the membrane material chromatography, and the detection line is clear and prominent. The support membrane commonly used as the chromatography main body is a nitrocellulose membrane, a cellulose acetate membrane, a nylon membrane, a polytetrafluoroethylene membrane, a glass cellulose membrane, or the like. In one example, a nitrocellulose membrane NC membrane is used in each of the lateral flow chromatography test strip detection zones.

As shown in FIG. 4, the length of the stabilizing channel 101 of the array plate 100 is greater than the length of the absorbent pad 301 and not greater than the total length of the absorbent pad 301, the chromatographic membrane 302 and the conjugate pad 303, and the length of the ramp 201 of the sample cartridge 200 is not less than the length of the sample pad 304. That is, the length of the sample cell 200 should be equal to or greater than the length of the sample pad 304 × cos θ, θ being the slope of the ramp 201, and it is ensured that the array plate 100 with the test strip 300 inserted therein can maintain a stable state of being obliquely inserted into the sample cell 200.

As an alternative embodiment, the length of the array plate 100 is equal to the total length of the absorbent pad 301, the chromatographic membrane 302 and the bonding pad 303, the upper surface of the stabilizing channel 101 has a sealed transparent film, and the array plate 100 is vacuum-molded. In this embodiment, the device of this application can develop into the product of disposable commercialization, and the length design of array board is for being equal to the total length of absorbent pad, chromatographic membrane, combination pad to seal transparent plastic film in the top subsides of firm channel 101, and carry out vacuum plastic envelope to array board 100, with the validity of guaranteeing the test paper strip more, prolong its life, a plurality of array boards can only be furnished with a sample liquid box, and sample liquid box used repeatedly.

As an alternative embodiment, the sample liquid box 200 has a baffle 202 above the ramp 201, and the baffle 202 is a strip-shaped plate horizontally arranged in the width direction. The array plate with the test paper has certain weight, the gravity center of the array plate is deviated to the water absorption pad end, the bottom of the array plate is lapped on one long edge of the sample liquid box when the array plate is inserted along the slope, a reverse supporting point is provided at the sample pad end by a baffle plate on the other long edge of the sample liquid box, the lever principle is met, the array plate is stabilized, and the sample pad end of the test paper strip is immersed in the sample liquid.

In one example, the baffle 202 is positioned above the sample fluid and the test strip 300. The height of the baffle 202 is selected by comprehensively considering the slope angle theta and the volume of the sample liquid, so that the baffle 202 is always higher than the level of the sample liquid; the width of the baffle 202 is selected according to the length of the test strip 300, and the test strip can be tilted stably and evenly by the reverse supporting force of the baffle 202.

As an alternative embodiment, the slope θ of the ramp 201 is 10 ° to 45 °.

In order to better explain the device of the present application, a specific embodiment is chosen for the description below.

The test strip array plate 100 is designed with a plurality of stable channels 101 for the test strips 300 to be inserted into, and the front ends of the stable channels 101 are designed with stoppers 102 having a certain thickness (i.e., height, e.g., 0.5mm in one example) to clamp the portions of the test strips 300 to be inserted into; the sample solution box 200 is designed with a slope 201 with a certain inclination (e.g. 10-45 degrees in one example) for the array plate 100 to slide in, and a baffle 202 with a certain length (e.g. a minimum 3mm and less than the length of the sample pad in one example) is designed on the side wall of the sample solution box 200 to stabilize the half-suspended part of the test strips 300 on the test strip array plate 100, so that the half-suspended parts of the test strips are always in contact with the sample solution.

The present application further provides the use of the apparatus described in any of the above embodiments, as follows:

(1) the plurality of test strips are inserted into the stable channel 101 with the matched width, the baffle plate 102 at the front end 1011 of the stable channel fixes the water absorption pad 301 end of the test strip 300, and one end of the sample pad 304 of the plurality of test strips 300 is kept flush and in a semi-suspension state;

(2) dripping a sample solution into a sample solution box 200 with a ramp 201 and a baffle 202 to enable the sample solution to be accumulated between the ramp 201 and the baffle 202;

(3) one ends of the sample pads 304 of the plurality of test strips are synchronously inserted into the sample box along the ramp 201 in the sample box 200, the test strips 300 are supported by the ramp 201 and are kept stable by the baffle 202, and the sample pads 304 of each test strip 300 are simultaneously soaked in the sample liquid for chromatography respectively.

In order to better understand the method of using the device of the present application, a specific embodiment is chosen and described below. Taking a 6-connection lateral flow chromatography test paper array device as an example, the water absorption pad ends of 6 test paper strips are respectively inserted into the test paper array plate along a stable channel and are stably clamped below respective stop blocks, the insertion distances are consistent, and the sample pad ends of the 6 test paper strips are kept in a parallel and level state; dropwise adding a sample solution to be analyzed into the sample solution box, and accumulating the sample solution below the baffle along the ramp; and inserting the semi-suspended sample pad of the test paper array plate into the sample solution box along the slope, and simultaneously immersing a plurality of test papers into the sample solution for chromatography.

The application has the advantages that the design keeps the detection line and the corresponding quality control line of each test paper, the real synchronous analysis of multiple test paper multiple targets can be realized by one-time sampling, the result deviation caused by mutual interference on a single test paper is avoided, the detection efficiency is improved to a certain extent, and the accuracy of the detection result is guaranteed.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the utility model. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. The horizontal synchronous fast-detection lateral flow chromatography test paper array device is characterized by comprising a test paper array plate (100) and a sample liquid box (200);

the test strip array plate (100) is provided with at least one stable channel (101) along the length direction, the two ends of the stable channel (101) are respectively a stable channel front end (1011) and a stable channel tail end (1012), the stable channel front end (1011) is closed, the stable channel tail end (1012) is open and used for inserting the test strip (300), wherein the water absorption pad (301) of the test strip (300) is inserted into and fixed at the stable channel front end (1011), and the sample pad (304) of the test strip (300) is hung outside the stable channel (101);

the periphery of the sample liquid box (200) is closed, the upper part of the sample liquid box is opened, a ramp (201) from high to low is arranged in the sample liquid box along the length direction, and the bottom of the ramp (201) is used for storing sample liquid;

when the test paper array device is in a test working state, the water absorption pad (301) of the test paper strip (300) is fixed at the front end (1011) of the stable channel, the test paper array plate (100) is moved, and the sample pad (304) of the test paper strip (300) slides downwards along the ramp (201) and is correspondingly inserted into the sample liquid at the bottom of the sample liquid box (200).

2. The horizontal synchronous rapid detection lateral flow chromatography test paper array device according to claim 1, wherein a stopper (102) in the shape of a cuboid is arranged at the upper position of the front end (1011) of the stable channel, and the lower surface of the stopper (102) is lower than the upper plane of the stable channel (101).

3. The horizontal synchronous rapid detection lateral flow chromatography test strip array device according to claim 2, wherein the sum of the height of the stopper (102) and the height of the test strip (300) is not less than the height of the stabilizing channel (101).

4. The horizontal synchronous rapid detection lateral flow chromatography test paper array device according to claim 1, wherein the width of each of the stable channels (101) is 1.1-1.3 times the width of the test paper strip (300), and the height of each of the stable channels (101) is 1.2-1.5 times the height of the test paper strip (300).

5. The horizontal synchronous rapid detection lateral flow chromatography test paper array device according to claim 1, wherein when the number of the stabilizing channels (101) is at least two, the stabilizing channels (101) are parallel and equidistantly arranged.

6. The horizontal synchronous rapid detection lateral flow chromatography test paper array device according to claim 1, wherein the test paper strip (300) comprises a water absorbent pad (301), a chromatography membrane (302), a binding pad (303) and a sample pad (304), the length of the stable channel (101) of the array plate (100) is greater than the length of the water absorbent pad (301) and not greater than the total length of the water absorbent pad (301), the chromatography membrane (302) and the binding pad (303), and the length of the ramp (201) of the sample solution box (200) is not less than the length of the sample pad (304).

7. The horizontal synchronous rapid detection lateral flow chromatography test paper array device according to claim 1, wherein the length of the array plate (100) is equal to the total length of the water absorption pad (301), the chromatography membrane (302) and the combination pad (303), the upper surface of the stable channel (101) is provided with a sealed transparent film, and the array plate (100) is subjected to vacuum plastic package.

8. The horizontal synchronous rapid detection lateral flow chromatography test paper array device according to claim 1, wherein the sample liquid box (200) is provided with a baffle (202) above the ramp (201), and the baffle (202) is a strip-shaped plate horizontally arranged along the width direction.

9. The horizontal synchronous rapid detection lateral flow chromatography test strip array device according to claim 8, wherein the baffle (202) is located above the sample solution and the test strip (300).

10. The horizontal synchronous fast detection lateral flow chromatography test paper array device according to claim 1, wherein the slope θ of the ramp (201) is 10-45 °.

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