CN215688452U - Kit for rapidly detecting exfoliated cell sample - Google Patents
Kit for rapidly detecting exfoliated cell sample Download PDFInfo
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- CN215688452U CN215688452U CN202122086432.7U CN202122086432U CN215688452U CN 215688452 U CN215688452 U CN 215688452U CN 202122086432 U CN202122086432 U CN 202122086432U CN 215688452 U CN215688452 U CN 215688452U
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Abstract
The utility model discloses a kit for rapidly detecting an exfoliated cell sample, which comprises a kit body and a reagent box cover. The kit body is provided with a placing cavity with an upward opening; the reagent lid sets up in the opening of placing the chamber, and the reagent lid is articulated with the back lateral wall of reagent box body, and the inside wall of reagent lid is provided with first baffle, and first baffle is first contained angle setting with the reagent lid, and first baffle is provided with the first test tube hole of a plurality of, is provided with bearing structure between reagent box body and the reagent lid, and bearing structure is used for making the reagent lid erect on placing the opening in chamber. The kit provided by the utility model has the effect of fixing a longer test tube under the condition of smaller volume, and is particularly suitable for an experiment in which a standing reaction test tube is required to wait for an experiment result in the experiment of quickly detecting the exfoliated cell sample.
Description
Technical Field
The utility model relates to the technical field of kits, in particular to a kit for rapidly detecting an exfoliated cell sample.
Background
Folic Acid (FA), also called vitamin B9, is an essential substance for nucleotide synthesis of eukaryotic cells, and animals cannot synthesize folic acid by themselves and only rely on the uptake and utilization of exogenous folic acid. Folate Receptors (FR) are cell surface glycoproteins, rich in cysteine, with high affinity for FA and folate derivatives, and mediate endocytic uptake. Folic acid, a water-soluble vitamin, is an important single-carbon donor involved in methionine metabolic pathway and DNA methylation, and is an important component of cellular metabolism and DNA synthesis and repair in vivo, and thus, the demand of rapidly dividing cancer cells for folic acid to maintain DNA synthesis is significantly increased. FR is over-expressed on the surfaces of various cancer cells such as mammary gland, ovary, lung, kidney, colon and brain, and is less expressed or not expressed on normal tissues and organs, and the expression level of FR on the cancer cells is 100-300 times of that of the normal tissues.
At present, immunocytochemistry is used as a method for detecting folate receptors in a sample of exfoliated cells. The method utilizes the characteristic that the antibody can be specifically combined with antigen, and then detects or locates a certain protein substance in tissues or cells through the color development of a marker on the antibody. However, the conventional immunocytochemistry technology takes a long time to complete the whole experiment, which results in that the detection of folate receptor expression by immunohistochemistry technology cannot obtain results timely, quickly and effectively, thus hindering the clinical application of cancer diagnosis. In order to solve the above problems, a method for detecting the expression of folate receptors on the surface of exfoliated cells has been proposed. When the method is used for detecting objects such as cell preservation solution, disposable cervical sampling brush (or swab), vat blue staining solution, 12ml centrifuge tube and 5ml transparent plastic tube, in the detection, the objects need to be taken from different places to detect, and in the detection process, the reaction result needs to be observed after the test tube is statically reacted for a period of time, and the test tube is not convenient to use because of the fact that the test tube is statically placed by means of an additional test tube rack.
SUMMERY OF THE UTILITY MODEL
The present invention is directed to a kit for rapidly detecting an exfoliated cell sample, which solves one or more of the problems of the prior art, and provides at least one of the advantages of the present invention.
The technical scheme adopted for solving the technical problems is as follows:
the utility model provides a kit for rapidly detecting an exfoliated cell sample, which comprises a kit body and a reagent box cover. The kit body is provided with a placing cavity with an upward opening; the reagent lid sets up in the opening of placing the chamber, and the reagent lid is articulated with the back lateral wall of reagent box body, and the inside wall of reagent lid is provided with first baffle, and first baffle is first contained angle setting with the reagent lid, and first baffle is provided with the first test tube hole of a plurality of, is provided with bearing structure between reagent box body and the reagent lid, and bearing structure is used for making the reagent lid erect on placing the opening in chamber.
The utility model has the beneficial effects that:
after the kit is opened, bearing structure makes the reagent lid erect in the kit body, and then the relative kit body bottom of first baffle is approximately the level setting, can place first test tube downthehole with the reaction test tube that needs to be used in the experiment this moment, plays the effect of fixed reaction test tube. And first baffle is higher for kit body bottom, can fix longer test tube. The kit provided by the utility model has the effect of fixing a longer test tube under the condition of smaller volume, and is particularly suitable for an experiment in which a standing reaction test tube is required to wait for an experiment result in the experiment of quickly detecting the exfoliated cell sample.
As the further improvement of the technical scheme, the inner side of the rear side wall of the kit body is provided with the second partition plate, the second partition plate and the rear side wall of the kit body are arranged at a second included angle, the second partition plate is provided with a plurality of second test tube holes, and the first test tube holes correspond to the second test tube holes one to one.
The test tube passes first test tube hole and second test tube hole simultaneously, plays better fixed action, also can make shorter test tube only pass first test tube hole and fix, and the second baffle plays the bearing effect, and then can fix the test tube of different specifications.
As above-mentioned technical scheme's further improvement, first contained angle and second contained angle all are 90 degrees, and first baffle and second baffle are parallel arrangement roughly, and it is more convenient to place the experiment test tube.
As a further improvement of the technical scheme, the supporting structure comprises a supporting rod which can freely stretch out and draw back, one end of the supporting rod is hinged with the reagent box cover, and the other end of the supporting rod is hinged with the reagent box body.
The support rod is simple in structure and good in support effect.
As a further improvement of the technical scheme, the number of the support rods is two, and the two support rods are respectively arranged at the left side and the right side of the kit body to play a better supporting role.
As above-mentioned technical scheme's further improvement, the bottom of placing the chamber is provided with two at least test tube snap rings, and two at least test tube snap rings are the interval and set up on the same straight line, can respectively the lock joint in the test tube snap ring to its both ends of longer experiment test tube, play the effect of fixed experiment test tube, damage the experiment test tube when avoiding removing the kit.
As a further improvement of the technical scheme, a sampling tool, a cell preservation solution reagent bottle, a folic acid receptor mediator bottle and a sealable reaction test tube are arranged in the placing cavity. The method is favorable for inspectors to quickly prepare reagents and supplies, and is convenient for directly implementing the method for detecting the expression of the folate receptor on the surface of the epithelial tissue cast-off cell.
As above-mentioned technical scheme's further improvement, it is provided with a plurality of fixed slots to place intracavity bottom, and cell preservation liquid reagent bottle, folic acid receptor mediation agent bottle, reaction test tube set up in the fixed slot one by one, and the inside wall of fixed slot is provided with the elastic block, and cell preservation liquid reagent bottle, folic acid receptor mediation agent bottle, reaction test tube and elastic block butt play the fixed action, damage experimental article when avoiding removing the kit.
As a further improvement of the technical scheme, the reaction test tube is a transparent test tube, so that the experimental result can be observed conveniently.
As a further improvement of the technical proposal, the reaction test tube is a plastic member or a glass member.
Drawings
The utility model is further described with reference to the accompanying drawings and examples;
FIG. 1 is a schematic structural diagram of an embodiment of the kit for rapid detection of an exfoliated cell sample provided by the present invention, wherein six arrows indicate forward, backward, left, right, upward and downward directions, respectively;
fig. 2 is a schematic top view of one embodiment of the kit for rapidly detecting an exfoliated cell sample provided in the present invention, wherein four arrows indicate a forward direction, a backward direction, a left direction and a right direction, respectively.
Detailed Description
Reference will now be made in detail to the present preferred embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to like elements throughout.
In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, if words such as "a plurality" are described, the meaning is one or more, the meaning of a plurality is two or more, more than, less than, more than, etc. are understood as excluding the present number, and more than, less than, etc. are understood as including the present number.
In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.
Referring to fig. 1 to 2, the kit for rapidly detecting an exfoliated cell sample of the present invention makes the following examples:
the method for rapidly detecting the exfoliated cell sample is a folic acid mediated vat blue staining solution staining technology, and is characterized by being rapid and efficient, and the method is used for detecting articles such as cell preservation solution, disposable cervical sampling brushes or swabs, vat blue staining solution, 12ml centrifuge tubes, 5ml transparent reaction test tubes 600 and the like.
Specifically, the experimental steps are as follows: 1. sampling of samples
1 opening the kit, taking out and using a disposable cervical sampling brush to sample the cervical part.
2, after sampling, quickly putting the sampling brush into the reagent bottle 400 filled with the cell preservation solution, breaking off the part higher than the bottle, and screwing the tube cover to finish sampling the sample.
2. Sample processing
1 placing a cell preservation solution reagent bottle 400 filled with a cell specimen into an oscillator to oscillate for 3-5 minutes;
2 sucking 1-2ml of cell preservation solution into a centrifuge tube by using a disposable pipette;
3800 g centrifugal force, centrifuging for 5min, slowly pouring out supernatant, and reserving sample cell precipitate;
4 sucking 1-2ml of PBS buffer solution to a centrifuge tube containing cell sediment by using a clean disposable pipette;
5, blowing and sucking by using a suction pipe or scattering cells at the bottom of the centrifugal pipe by using a vortex mixer so as to prepare cell suspension;
3. dyeing with vat blue dyeing liquid
1 sucking 2ml of staining solution into a transparent reaction test tube 600 by using a clean disposable pipette, sucking the cell suspension by using the clean disposable pipette, dropwise adding 3-5 drops of cell amount into the staining solution in the reaction test tube 600, standing for 30s, reversely shaking the reaction test tube 6005, and standing for 10 min;
2 observing whether blue color appears on the wall of the reaction tube 600, and judging the result.
In some embodiments, the kit for rapidly detecting the exfoliated cell sample comprises a kit body 100 and a reagent kit cover 200. The cartridge body 100 is provided with a placing chamber 130 with an upward opening. A sampling tool, a cell preservation solution reagent bottle 400, a folic acid receptor mediating agent bottle 500 and a sealable reaction test tube 600 are arranged in the placing cavity 130, working liquid required by an experiment is prepared and then placed in the placing cavity 130, and experimental related equipment is prepared. Can effectively shorten the time, thereby achieving the effect of quickly and conveniently detecting the expression of the folate receptor on the surface of the exfoliated cell.
In order to reduce the phenomenon that the experimental article is damaged due to the fact that the experimental article shakes in the reagent box cover 200, a plurality of fixing grooves are formed in the bottom of the placing cavity 130, the specific shapes of the fixing grooves are matched with the fixed experimental article, for example, the folic acid receptor mediator bottle 500 is cylindrical, and the fixing grooves can be formed into cylindrical concave cavities, so that the folic acid receptor mediator bottle 500 is clamped in the fixing grooves. Cell preservation liquid reagent bottle 400, folic acid receptor mediation agent bottle 500, reaction test tube 600 set up one by one in the fixed slot, and the inside wall of fixed slot is provided with the elastic block, and cell preservation liquid reagent bottle 400, folic acid receptor mediation agent bottle 500, reaction test tube 600 and elastic block butt, the elastic block plays the fixed action, damages experimental article when avoiding removing the kit. In some other embodiments, the bottom of placing chamber 130 is provided with at least two test tube snap rings 120, and at least two test tube snap rings 120 are the interval and set up on the same straight line, can respectively the lock joint in test tube snap ring 120 to its both ends of longer experiment test tube, play the effect of fixed experiment test tube, damage the experiment test tube when avoiding removing the kit.
The experimental result of the experimental method is judged by observing whether blue color appears on the tube wall of the reaction test tube 600. Therefore, the reaction tube 600 is a transparent tube, which facilitates observation of the experimental results. The reaction cuvette 600 is a plastic member or a glass member.
Specifically, the supporting structure 300 includes two supporting rods 310 capable of freely extending and retracting, the supporting rods 310 are simple in structure, good in supporting effect and convenient to use, and the reagent box cover 200 can be supported by opening the reagent box cover 200. One end of the support rod 310 is hinged with the reagent box cover 200, and the other end of the support rod 310 is hinged with the reagent box body 100. The two support rods 310 are respectively disposed on the left and right sides of the reagent cartridge body 100 to better support. It should be noted that the number of the support rods 310 is not limited in the present invention.
After the reagent box is opened, the supporting structure 300 makes the reagent box cover 200 stand on the reagent box body 100, and the first partition board 210 is approximately horizontally arranged relative to the bottom of the reagent box body 100, so that the reaction test tube 600 required in the experiment can be placed in the first test tube hole 211, and the effect of fixing the reaction test tube 600 is achieved. And the first partition 210 is higher with respect to the bottom of the cartridge body 100, and can fix a longer test tube.
Further, in order to better place the test tube, the inboard of lateral wall sets up second baffle 110 behind kit body 100, and second baffle 110 is the setting of second contained angle with kit body 100 rear side wall, and the setting of second contained angle is 90 degrees settings, and second baffle 110 is provided with three second test tube hole 111, first test tube hole 211 and second test tube hole 111 one-to-one.
The test tube passes first test tube hole 211 and second test tube hole 111 simultaneously, and the test tube is difficult for taking place to incline, especially fixed longer test tube can play better fixed action, also can make shorter test tube only pass first test tube hole 211 and fix, and second baffle 110 plays the bearing effect, and then can fix the test tube of different specifications. In the experimental process, run into and reverse the experiment test tube of rocking, then when standing still the operating procedure of a period of time, can open the kit for first baffle 210 and second baffle 110 are roughly the form of test-tube rack, and the test tube is placed on first baffle 210 and second baffle 110, need not additionally to use solitary test-tube rack again, and is more convenient in the experimental process.
All related reagents in the kit are prepared working liquid and are also provided with consumables for sampling, processing and dyeing, and the reaction test tube 600 can be fixed in the kit when standing for a result in the processing process, so that the operation steps are simple and convenient, and compared with the conventional immunocytochemistry dyeing technology, the kit can effectively shorten the time, thereby achieving the effect of quickly and conveniently detecting the expression of the folate receptor on the surface of the exfoliated cell.
While the preferred embodiments of the present invention have been described in detail, it is to be understood that the utility model is not limited to the precise embodiments, and that various equivalent changes and modifications may be effected therein by one skilled in the art without departing from the spirit and scope of the utility model as defined in the appended claims.
Claims (10)
1. A kit for rapidly detecting an exfoliated cell sample, comprising:
a cartridge body (100) provided with a placement chamber (130) having an upward opening;
reagent box lid (200), it sets up in the opening of placing chamber (130), reagent box lid (200) with the back lateral wall of reagent box body (100) is articulated, the inside wall of reagent box lid (200) is provided with first baffle (210), first baffle (210) with reagent box lid (200) is the setting of first contained angle, first baffle (210) are provided with the first test tube hole of a plurality of (211), reagent box body (100) with be provided with bearing structure (300) between reagent box lid (200), bearing structure (300) are used for making reagent box lid (200) erect in place on the opening of chamber (130).
2. The kit for rapidly detecting an exfoliated cell sample as claimed in claim 1, wherein:
the inner side of the rear side wall of the kit body (100) is provided with a second partition plate (110), the second partition plate (110) and the rear side wall of the kit body (100) are arranged at a second included angle, the second partition plate (110) is provided with a plurality of second test tube holes (111), and the first test tube holes (211) are in one-to-one correspondence with the second test tube holes (111).
3. The kit for rapidly detecting an exfoliated cell sample as claimed in claim 2, wherein:
the first included angle and the second included angle are both 90 degrees.
4. The kit for rapidly detecting an exfoliated cell sample as claimed in claim 1, wherein:
the supporting structure (300) comprises a supporting rod (310) which can freely stretch out and draw back, one end of the supporting rod (310) is hinged with the reagent box cover (200), and the other end of the supporting rod (310) is hinged with the reagent box body (100).
5. The kit for rapidly detecting an exfoliated cell sample as claimed in claim 4, wherein:
the number of the support rods (310) is two, and the two support rods (310) are respectively arranged on the left side and the right side of the kit body (100).
6. The kit for rapidly detecting an exfoliated cell sample as claimed in claim 1, wherein:
the bottom of placing chamber (130) is provided with two at least test tube snap rings (120), two at least test tube snap ring (120) are the interval and set up on the same straight line.
7. The kit for rapidly detecting an exfoliated cell sample as claimed in claim 1, wherein:
a sampling tool, a cell preservation solution reagent bottle (400), a folic acid receptor mediating agent bottle (500) and a sealable reaction test tube (600) are arranged in the placing cavity (130).
8. The kit for rapidly detecting an exfoliated cell sample as claimed in claim 7, wherein:
place intracavity (130) bottom and be provided with a plurality of fixed slots, cell preservation liquid reagent bottle (400) folic acid receptor mediator bottle (500) reaction test tube (600) set up one by one in the fixed slot, the inside wall of fixed slot is provided with the elastic block, cell preservation liquid reagent bottle (400), folic acid receptor mediator bottle (500) reaction test tube (600) with the elastic block butt.
9. The kit for rapidly detecting an exfoliated cell sample as claimed in claim 7, wherein:
the reaction tube (600) is a transparent tube.
10. The kit for rapidly detecting an exfoliated cell sample as claimed in claim 9, wherein:
the reaction cuvette (600) is a plastic member or a glass member.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122086432.7U CN215688452U (en) | 2021-08-31 | 2021-08-31 | Kit for rapidly detecting exfoliated cell sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122086432.7U CN215688452U (en) | 2021-08-31 | 2021-08-31 | Kit for rapidly detecting exfoliated cell sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215688452U true CN215688452U (en) | 2022-02-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122086432.7U Active CN215688452U (en) | 2021-08-31 | 2021-08-31 | Kit for rapidly detecting exfoliated cell sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215688452U (en) |
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2021
- 2021-08-31 CN CN202122086432.7U patent/CN215688452U/en active Active
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