JP2009300433A5

JP2009300433A5 -

Info

Publication number: JP2009300433A5
Application number: JP2009118440A
Authority: JP
Filing date: 2009-05-15
Publication date: 2012-06-28
Anticipated expiration: 2029-05-15

Claims

An analysis chip for performing immunological analysis by repeatedly rotating and feeding a sample and a plurality of reagents to a reaction chamber unit having a reaction chamber capable of accommodating a carrier bound with an antigen or an antibody,
(1) The insoluble component in the suspension as a specimen can be separated from the separated solution by the action of centrifugal force and settled, and the separated solution is separated by the action of gravity, and then the action of the centrifugal force. An insoluble component separator that can be transferred to the reaction chamber unit;
(2) Two or more tanks and a flow path connecting the tanks are provided, and by the action of centrifugal force and gravity, the reagent is sequentially and / or simultaneously sent between adjacent tanks through the flow path, and the reaction An analysis chip having a multistage liquid feeding unit capable of feeding liquid to a chamber unit.

2. The analysis according to claim 1, wherein the repetition of the rotation is a repetition of a rotation at a first rotation speed and a rotation at a rotation speed slower than the rotation speed by the first rotation speed or a second rotation speed that is a rotation stop. Chip.

The multistage liquid feeding section is
A first reservoir capable of introducing a reagent when rotation of the analysis chip is stopped;
A first holding tank located on the outer peripheral side of the rotation of the first liquid storage tank;
A second holding tank located in the direction of gravity of the first holding tank;
A flow path A communicating between the first liquid storage tank and the first holding tank;
A flow path B extending from the first holding tank in the direction of gravity and communicating with the first holding tank and the second holding tank;
The first holding tank, the flow path B, and the second holding tank are used as one liquid feeding unit, and two or more liquid feeding units are connected and arranged,
A connection between adjacent liquid-feeding units extends from the second holding tank of the upper liquid-feeding unit to the outer peripheral side of the rotation and communicates with the first holding tank of the lower liquid-feeding unit. By C,
The second holding tank and the reaction chamber unit of the lowermost liquid-feeding unit communicate with each other, and extend from the second holding tank of the lowermost liquid-feeding unit in the outer circumferential direction with reference to the rotation axis. And further comprising a flow path D connected to the reaction chamber unit,
The analysis chip according to claim 1 or 2 .

The first holding tank is a tank capable of holding a reagent in the first holding tank when rotating at the first rotation speed of the analysis chip.
The flow path B uses the action of centrifugal force and gravity at the time of rotation at the second rotation speed that is lower than the first rotation speed of the analysis chip or when the rotation is stopped. A flow path through which the reagent inside can be passed and moved to the second holding tank,
The analysis chip according to claim 3 , wherein the second holding tank is capable of holding a reagent in the second holding tank when the analysis chip is rotated or stopped at the second rotation speed.

The analysis chip according to claim 3 or 4 , wherein the flow path B is bent toward the outer periphery of the rotation in the middle of the flow path.

At least one of the plurality of liquid feeding units is the liquid feeding unit,
A second liquid storage tank located on the inner peripheral side of the second holding tank;
The second holding tank, the the first storage tank or the first holding tank, any one of claims 3-5, further comprising a flow passage E that communicates with the second storage tank The analysis chip according to item.

The analysis chip according to any one of claims 3 to 6 , wherein different reagents are stored in advance in at least two of the second storage tanks included in the analysis chip.

The insoluble component separation part is
A suspension holding tank, a separation liquid holding tank, an insoluble component holding tank, and a separation liquid feeding path;
The suspension holding tank, the separation liquid holding tank, and the insoluble component holding tank are arranged in this order from the inner peripheral side during rotation,
The suspension holding tank and the insoluble component holding tank are connected,
The insoluble component holding tank and the separation liquid holding tank are connected by a narrow portion,
In the insoluble component holding tank, the connection part with the suspension holding tank is located on the outer peripheral side from the narrow part,
The separation liquid feeding path extends in the direction of gravity from the separation liquid holding tank and communicates with the reaction chamber unit.
The analysis chip according to any one of claims 1 to 7 .

The analysis chip according to claim 8 , wherein a connection portion of the insoluble component holding tank with the suspension holding tank is located on an outer peripheral side wall surface of the insoluble component holding tank.

The analysis chip according to claim 8 or 9 , wherein the separation liquid supply path extends in the direction of gravity and on the outer peripheral side.

The analysis chip according to any one of claims 8 to 10 , wherein the suspension holding tank and the insoluble component holding tank are connected by a suspension introduction path.

An overflow channel connected to the suspension holding tank, the suspension introduction path or the insoluble component holding tank is further provided, and the overflow channel is connected to the suspension introduction path or the insoluble component holding tank. The analysis chip according to claim 11 , wherein the analysis chip has a structure in which the connection portion is once extended to the inner peripheral side and then folded back to the outer peripheral side.

The insoluble component separation unit, the analyzing and holding the reagent during rotation of the chip, any one of claims 8 to 12, by the action of gravity when rotation is stopped with the reagent holding tank for discharging the reagent to the separation liquid holding tank The analysis chip according to item.

The analysis chip according to claim 13 , further comprising a reagent storage tank connected to a reagent holding tank and configured to store a reagent to be fed when the analysis chip is rotated.

The analysis chip according to any one of claims 8 to 14 , wherein a prefilter part is provided in the separation liquid feeding path.

One or more tanks selected from the reagent storage tank of the insoluble component separation part and the second storage tank of the multistage liquid supply part are provided in a reagent reservoir unit that is removable from the chip body. The analysis chip according to any one of claims 6 to 15 .

The analysis chip according to claim 16 , wherein the reagent reservoir unit is detachably provided on a rotation inner peripheral side of the chip body.

Analysis chip according to claim 16 or 17 wherein the reagent liquid storage reagent in the reservoir provided in the reservoir unit, characterized in that to move the respective tanks of the chip body upon rotation of the analysis chip.

19. At least a part of the insoluble component separation part and the multistage liquid feeding part are provided apart from each other near two main surfaces facing each other, according to any one of claims 1 to 18. The analysis chip described.

The analysis chip according to claim 19 , wherein at least a part of the reaction chamber unit is provided near a surface other than the two main surfaces.

The analysis chip according to any one of claims 1 to 20 is repeatedly rotated with respect to a rotation axis outside the analysis chip, thereby sending a sample and a reagent to the reaction chamber of the analysis chip, An analysis method comprising measuring the amount of a test substance in the reaction chamber.

(1) Suspension which is a sample is introduced into the analysis chip according to any one of claims 1 to 20 , and insoluble components are precipitated using centrifugal force generated when the analysis chip is rotated. After that, stop the rotation and use gravity to separate the separated liquid,
(2) Using the centrifugal force and gravity generated when the analysis chip is rotated, the reagent is fed to the multistage liquid feeding unit,
(3) The analysis method according to claim 21 , wherein the separation liquid and the reagent are brought into contact with a carrier to which an antigen or an antibody is bound using a centrifugal force generated by rotation.

The analysis method according to claim 22 , wherein the specimen is blood, the insoluble component is a blood cell component, and the separation liquid is serum or plasma.