CN215627877U - Rare cell multi-stage sorting micro-fluidic device - Google Patents

Abstract

The utility model discloses a multi-stage rare cell sorting micro-fluidic device which comprises a micro-fluidic device main body, wherein one end of the micro-fluidic device main body is fixedly provided with a stirrer, a water inlet of the stirrer is fixedly provided with a conveying pipe, the end part of the conveying pipe is fixedly provided with a pressure pump, a fluorescent sieve is embedded in the conveying pipe, one end of the inner bottom surface of the micro-fluidic device main body is fixedly provided with a sorting framework, the other end of the inner bottom surface of the micro-fluidic device main body is fixedly provided with a sorting channel, the sorting channel comprises a plurality of vertically fixed sorting auxiliary channels, the inner top surfaces of the sorting auxiliary channels are respectively provided with an optical sensor, and the other ends of the sorting auxiliary channels are respectively provided with an electric two-way valve. This micro-fluidic device is selected separately to rare cell multistage simple structure, the modern design, convenient to use has accelerated mass transfer process and liquid mixing process greatly, and then has greatly improved cell separation efficiency, has shortened cell and has selected separately for a long time, conveniently selects separately once more to the not good cell solution of sorting effect simultaneously, has improved the sorting effect greatly.

Description

Rare cell multi-stage sorting micro-fluidic device

Technical Field

The utility model belongs to the technical field of cell sorting, and particularly relates to a rare cell multistage sorting microfluidic device.

Background

Microfluidics (Microfluidics), which refers to the science and technology involved in systems using microchannels (tens to hundreds of microns in size) to process or manipulate tiny fluids (nanoliters to attoliters in volume), is an emerging interdiscipline of chemistry, fluid physics, microelectronics, new materials, biology and biomedical engineering. Because of their miniaturization, integration, etc., microfluidic devices are commonly referred to as microfluidic chips, also known as lab-on-a-chip and micro total analysis systems. The early concept of microfluidics can be traced back to gas chromatographs fabricated on silicon wafers by photolithography in the 70 s of the 19 th century, and then developed into microfluidic capillary electrophoresis instruments, microreactors and the like. One of the important features of microfluidics is the unique fluid properties in microscale environments, such as laminar flow and droplets. With these unique fluidic phenomena, microfluidics can achieve a range of microfabrication and micromanipulation that are difficult to accomplish with conventional methods. Microfluidics is currently considered to have great development potential and broad application prospects in biomedical research. However, the existing microfluidic device is often simple in structure, the liquid mixing process and the mass transfer process under laminar flow are relatively slow, so that the cell sorting time is increased, and the defect of poor sorting effect exists.

Disclosure of Invention

The utility model aims to provide a rare cell multistage sorting microfluidic device to solve the problems of long sorting time and poor sorting effect of the conventional microfluidic device.

In order to achieve the purpose, the utility model provides the following technical scheme: the utility model provides a rare cell multistage separation micro-fluidic device, includes the micro-fluidic device main part, the micro-fluidic device main part is cuboid cavity structure, the one end of micro-fluidic device main part is fixed with the agitator, the delivery port of agitator extends to the micro-fluidic device main part inside, the water inlet of agitator is fixed with the conveyer pipe, the end fixing of conveyer pipe has the force pump, the last fixed surface of conveyer pipe has injection tube and back flow, injection tube and back flow all link up with the conveyer pipe, the conveyer pipe is embedded to have fluorescence sieve, the one end of bottom surface is fixed with the separation skeleton in the micro-fluidic device main part, and the other end is fixed with the separation passageway, the separation passageway includes a plurality of vertical fixation's separation auxiliary channel, the interior top surface of separation auxiliary channel all is equipped with optical sensor, and the other end all is equipped with electronic two-way valve, the end of the electric two-way valve is respectively connected with a discharge pipe and a return branch pipe, and the return branch pipes are connected with a return pipe.

Preferably, the upper surface of micro-fluidic device main part is equipped with liquid crystal display and function button, the function button includes that the device opens and stops button, valve shift knob and backwash pump and open and stop the button, the interior top surface of micro-fluidic device main part is equipped with the singlechip, what the singlechip adopted is C general type singlechip.

Preferably, all be equipped with the backwash pump on the backward flow branch pipe, the pivot is connected with agitator motor on the agitator, force pump, optical sensor, electronic two-way valve, liquid crystal display, function button, backwash pump and agitator motor all with singlechip electric connection.

Preferably, the cross section of the fluorescent screen is circular and is matched with the inner diameter of the conveying pipe, and the fluorescent screen is vertically arranged between the perpendicular bisectors of the injection pipe and the return pipe.

Preferably, the sorting framework is an asymmetric fishbone-shaped protruding structure, is made of glass, and is integrally designed with the microfluidic device main body.

Preferably, the microfluidic device main body and the sorting channel are made of glass.

The utility model has the technical effects and advantages that: this micro-fluidic device is selected separately to rare cell multistage simple structure, the modern design, high durability and convenient use, through setting up the force pump, agitator and separation skeleton, mass transfer process and liquid mixing process have been accelerated greatly, and then cell sorting efficiency has greatly been improved, it is long when having shortened the cell sorting, through setting up fluorescence sieve, optical sensor and liquid crystal display, conveniently observe the separation effect, through setting up the back flow, backward flow branch pipe and backwash pump, conveniently select separately once more to the not good cell solution of separation effect, the separation effect has been improved greatly.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a horizontal cross-sectional view of the present invention;

FIG. 3 is a vertical cross-sectional view of a sorting channel of the present invention;

fig. 4 is a horizontal cross-sectional view of a sorting sub-channel of the present invention.

In the figure: 1. a microfluidic device body; 2. a pressure pump; 3. a delivery pipe; 4. an injection pipe; 5. a fluorescent screen; 6. a return pipe; 7. a stirrer; 8. a sorting channel; 9. sorting the framework; 10. a sorting sub-channel; 11. an optical sensor; 12. an electric two-way valve; 13. a discharge pipe; 14. a return branch pipe; 15. a liquid crystal display screen; 16. a function button; 17. a reflux pump; 18. a stirring motor; 19. and a single chip microcomputer.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The utility model provides a rare cell multistage sorting micro-fluidic device as shown in the figure, which comprises a micro-fluidic device main body 1, wherein the micro-fluidic device main body 1 is of a cuboid cavity structure, one end of the micro-fluidic device main body 1 is fixedly provided with a stirrer 7, a water outlet of the stirrer 7 extends into the micro-fluidic device main body 1, a water inlet of the stirrer 7 is fixedly provided with a conveying pipe 3, the end part of the conveying pipe 3 is fixedly provided with a pressure pump 2, the upper surface of the conveying pipe 3 is fixedly provided with an injection pipe 4 and a return pipe 6, the injection pipe 4 and the return pipe 6 are both communicated with the conveying pipe 3, a fluorescent sieve 5 is embedded in the conveying pipe 3, one end of the inner bottom surface of the micro-fluidic device main body 1 is fixedly provided with a sorting framework 9, the other end of the inner bottom surface of the micro-fluidic device main body is fixedly provided with a sorting channel 8, the sorting channel 8 comprises a plurality of vertically fixed sorting sub-channels 10, the inner top surfaces of the sorting auxiliary channels 10 are respectively provided with an optical sensor 11, the other ends of the sorting auxiliary channels are respectively provided with an electric two-way valve 12, the end parts of the electric two-way valves 12 are respectively connected with a discharge pipe 13 and a return branch pipe 14, and the return branch pipes 14 are respectively connected with a return pipe 6.

In addition, the upper surface of micro-fluidic device main part 1 is equipped with liquid crystal display 15 and function button 16, function button 16 opens and stops button, valve shift knob and backwash pump and open and stop the button including the device, the interior top surface of micro-fluidic device main part 1 is equipped with singlechip 19, what singlechip 19 adopted is 80C51 general type singlechip, conveniently through 16 input control instructions of function button, handles the back through singlechip 19 and sends the instruction to concrete part, makes things convenient for the staff to operate, through setting up liquid crystal display 15 convenient clear directly perceived viewing device running state. The reflux branch pipes 14 are respectively provided with a reflux pump 17, the stirrer 7 is connected with a stirring motor 18 through an upper shaft, and the pressure pump 2, the optical sensor 11, the electric two-way valve 12, the liquid crystal display screen 15, the function button 16, the reflux pump 17 and the stirring motor 18 are electrically connected with the single chip microcomputer 19, so that all the components are uniformly coordinated and operated under the control of the single chip microcomputer 19 to form a functional whole.

It is worth mentioning that the cross section of fluorescence sieve 5 is circular and with the internal diameter phase-match of conveyer pipe 3, fluorescence sieve 5 sets up perpendicularly between the perpendicular bisector of injection pipe 4 and back flow 6, and the aperture of fluorescence sieve 5 is the same with the diameter of the rare cell of waiting to sort, conveniently treats the rare cell of sorting and dyes, is favorable to avoiding carrying out the secondary dyeing to the cell of sorting once more in the back flow 6 simultaneously. The sorting framework 9 is of an asymmetric fishbone-shaped protruding structure, is made of glass, and is integrally designed with the micro-fluidic device main body 1, so that the mass transfer process and liquid mixing can be accelerated, the sorting efficiency can be improved, and the sorting time can be shortened. The micro-fluidic device main body 1 and the sorting channel 8 are made of glass, are cheap and easy to obtain, and are convenient to observe the internal operation condition of the device.

The working principle is as follows: when the multi-stage rare cell sorting microfluidic control device is used, firstly, a cell solution to be sorted is injected into a conveying pipe 3 through an injection pipe 4, then the device is started through a function button 16, at the moment, a pressure pump 2 is started to push the cell solution to be sorted to the other side of the conveying pipe 3, the cell solution dyes rare cells matched with the aperture of a fluorescent screen 5 when passing through the fluorescent screen 5, then the cell solution to be sorted enters a microfluidic control device main body 1 after being stirred by a stirrer 7, then the mass transfer process and liquid mixing are accelerated through a sorting framework 9, then different laminar flows of the cell solution to be sorted sequentially enter sorting auxiliary channels 10, then the rare cells in the cell solution are detected through an optical sensor 11, the detection result is displayed on a liquid crystal display screen 15, and then an electric two-way valve 12 on a discharge pipe 13 corresponding to the sorting auxiliary channel 10 with high content of the rare cells is opened, discharging rare cells, opening an electric two-way valve 12 on a reflux branch pipe 14 corresponding to a sorting auxiliary channel 10 with lower rare cell content, and then starting a reflux pump 17 to convey cell solution to a reflux pipe 6 for re-sorting.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the utility model.

Claims (6)

1. The utility model provides a rare cell multistage sorting micro-fluidic device, includes micro-fluidic device main part (1), its characterized in that: the utility model discloses a sorting device, including microfluidic device main part (1), micro-fluidic device main part (1) is cuboid cavity structure, the one end of microfluidic device main part (1) is fixed with agitator (7), the delivery port of agitator (7) extends to inside microfluidic device main part (1), the water inlet of agitator (7) is fixed with conveyer pipe (3), the end fixing of conveyer pipe (3) has force pump (2), the last fixed surface of conveyer pipe (3) has injection tube (4) and back flow (6), injection tube (4) and back flow (6) all with conveyer pipe (3) through connection, conveyer pipe (3) are embedded to have fluorescence sieve (5), the one end of bottom surface is fixed with sorting framework (9) in microfluidic device main part (1), and the other end is fixed with sorting channel (8), sorting channel (8) include a plurality of vertical fixation's sorting auxiliary channel (10), the inner top surface of the sorting auxiliary channel (10) is provided with an optical sensor (11), the other end of the sorting auxiliary channel is provided with an electric two-way valve (12), the end part of the electric two-way valve (12) is respectively connected with a discharge pipe (13) and a return branch pipe (14), and the return branch pipe (14) is connected with a return pipe (6).

2. The microfluidic device for multistage sorting of rare cells according to claim 1, wherein: the upper surface of micro-fluidic device main part (1) is equipped with liquid crystal display (15) and function button (16), function button (16) are opened and is stopped button, valve shift knob and backwash pump and open and stop the button including the device, the interior top surface of micro-fluidic device main part (1) is equipped with singlechip (19), what singlechip (19) adopted is the general type singlechip of 80C 51.

3. The microfluidic device for multistage sorting of rare cells according to claim 2, wherein: all be equipped with backwash pump (17) on backward flow branch pipe (14), the last hub connection of agitator (7) has agitator motor (18), force pump (2), optical sensor (11), electronic two-way valve (12), liquid crystal display (15), function button (16), backwash pump (17) and agitator motor (18) all with singlechip (19) electric connection.

4. The microfluidic device for multistage sorting of rare cells according to claim 1, wherein: the cross section of the fluorescent screen (5) is circular and is matched with the inner diameter of the conveying pipe (3), and the fluorescent screen (5) is vertically arranged between perpendicular bisectors of the injection pipe (4) and the return pipe (6).

5. The microfluidic device for multistage sorting of rare cells according to claim 1, wherein: the sorting framework (9) is of an asymmetric fishbone-shaped protruding structure, is made of glass, and is integrally designed with the microfluidic device main body (1).

6. The microfluidic device for multistage sorting of rare cells according to claim 1, wherein: the micro-fluidic device main body (1) and the sorting channel (8) are both made of glass.

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