CN215530668U - Combined tissue culture device - Google Patents

Abstract

The utility model relates to a combined tissue culture device which comprises a square box body, a box cover and a plurality of culture bottles, wherein each culture bottle comprises a bottomless square lower bottle body and an upper bottle body, a clamping hole is formed in the box cover, the square lower bottle body of each culture bottle is arranged in the square box body and is tightly contacted with the inner wall of the square box body, the upper bottle body of each culture bottle is arranged together with the box cover through the clamping hole, the square box body and the box cover are sealed together, and the height of the square box body is 1/6-1/3 the height of each culture bottle. The device simple structure of this embodiment can realize the even quick partial shipment of culture medium, the work effect that improves effectively, the precision of the experiment of improvement.

Description

Combined tissue culture device

Technical Field

The utility model relates to a combined tissue culture device, and belongs to the field of tissue culture equipment.

Background

Tissue culture of plants refers to a technique of separating desired tissues, organs or cells, protoplasts, etc. from plant bodies, and inoculating them under aseptic conditions to a medium containing various nutrients and plant hormones for culture to obtain regenerated whole plants or produce other products having economic value by aseptic manipulation. In the process of plant culture in a laboratory, a culture medium is prepared according to the nutritional requirements of plant tissue culture, then the culture medium is uniformly mixed and is subpackaged into culture bottles, and then sterilization treatment is carried out. The above process has the following problems: (1) in the culture medium mixing process, uneven mixing is easy to occur, and particularly, a raw material with poor dispersibility is easy to cause the culture medium components in each culture bottle after subpackaging to be different; (2) in the process of subpackaging the culture medium, the subpackaging workload is large, and the subpackaging amount is different; (3) during the plant tissue culture process, the cultured plant needs to be photographed. But current blake bottle is erlenmeyer flask or round bottle, and the blake bottle surface is the arcwall face in the in-process of shooing, reflects light easily, causes and shoots not clear scheduling problem.

SUMMERY OF THE UTILITY MODEL

The utility model provides a combined tissue culture device, which solves the problems of uneven mixing and different subpackaging amount in the preparation and subpackaging processes of the existing culture medium and the problem that the existing culture bottle is not easy to photograph.

In order to solve the technical problems, the technical scheme adopted by the utility model is as follows:

the utility model provides a modular tissue culture device, includes square box body, lid and a plurality of blake bottle, the blake bottle including the square lower bottle body and the last bottle body of bottomless, the lid on be provided with the card hole, the square lower bottle body of toper blake bottle install in square box body, and with the inner wall in close contact with of square box body, the last bottle body of toper blake bottle pass through the card hole with the lid install together, square box body and lid sealed together, square box body highly be 1/6-1/3 of blake bottle height.

Preferably: the square box body is provided with a box body handle, and the box cover is provided with a box cover handle.

Preferably: the upper bottle body is provided with a fixing bulge, and the box body is provided with a fixing groove.

Preferably: the inner edge of the box cover is provided with a sealing layer.

Preferably: the combined tissue culture device also comprises a lock catch, wherein the fixed part of the lock catch is arranged on the box cover, and the movable part of the lock catch is arranged on the square box body.

Preferably: the combined tissue culture device further comprises a light transmission grating, wherein clamping holes are formed in the light transmission grating, the light transmission grating is arranged between the square lower bottle bodies, and the height of the light transmission grating is consistent with that of the square lower bottle bodies.

Preferably: the combined tissue culture device also comprises a flexible box cover lining and a flexible box body lining.

Preferably: the flexible box cover lining and the flexible box body lining are made of polyethylene materials or silica gel materials.

The utility model has the beneficial effects that:

the utility model adopts the structure of the square box body, the box cover and the culture bottles, when in use, the culture bottles are integrally pulled high, the culture medium is poured into the square box body after being prepared, the culture medium is sterilized, and the culture bottles are pushed into the square box body after being sterilized, so that the culture medium enters the culture bottles through the bottle bottoms, and further the split charging of the culture medium is realized. The structure has the following advantages: (1) after the culture medium raw materials are mixed, the raw materials are sterilized in a uniform manner in a square box body, so that the problem that the nutrient components of the culture medium are inconsistent due to nonuniform mixing and subpackaging of the culture medium can be effectively avoided; (2) a plurality of culture bottles are horizontally pushed into the square box body simultaneously, so that the culture medium uniformly enters the culture bottles, the volume of the culture medium entering the culture bottles is consistent, and the problem of nonuniform subpackage of the culture medium can be effectively avoided; (3) a plurality of blake bottle carries out the partial shipment simultaneously, can effectual raising the efficiency, reduces artifically.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic structural view of example 1 of the present invention;

FIG. 2 is a schematic view of the structure of FIG. 1 along line A-A;

FIG. 3 is a schematic view of the structure of FIG. 1 along the line B-B;

FIG. 4 is an enlarged view of section C of FIG. 1;

FIG. 5 is a state diagram of the use of FIG. 1;

FIG. 6 is a schematic structural view of embodiment 2 of the present invention;

FIG. 7 is a schematic view of the structure of FIG. 6 along line A-A;

FIG. 8 is a schematic view of the structure of FIG. 6 along direction B-B;

FIG. 9 is a state diagram of the use of FIG. 6;

FIG. 10 is a schematic structural view of example 3 of the present invention; in the figure, 1 is a box cover handle, 2 is a box cover, 3 is a box body handle, 4 is a square box body, 5 is a round upper bottle body, 6 is a square lower bottle body, 7 is a fixed bulge, 8 is a sealing layer, 9 is a light-transmitting grid, 10 is a square upper bottle body, 11 is a fixed part, 12 is a movable part, 13 is a flexible box cover lining, 14 is a flexible box body lining

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

As shown in FIGS. 1 to 5, a combined tissue culture device comprises a square box body 4, a box cover 2 and a plurality of culture bottles,

the blake bottle include square bottle 6 and the last bottle down of bottomless, lid 2 on be provided with the card hole, square bottle 6 under of blake bottle install in square box body 4, and with the inner wall in close contact with of square box body 4, the last bottle of toper blake bottle pass through the card hole with lid 2 install together, square box body 4 and lid 2 sealed together, square box body 4 highly be 1/6-1/3 of blake bottle height.

In this embodiment, a conical flask is taken as an example, the upper flask body in this embodiment is a circular upper flask body 5, the square box body 4 is adapted to the square lower flask body 6, and the square box body 4 contacts with the square lower flask body 6, so that the mixed bacteria can enter the culture flask in the culture process effectively, and further pollute the culture medium. The height of the square box 4 affects the lighting degree of the culture bottle, and cannot be too high, and is generally slightly larger than the bottling amount height of the culture bottle, and is generally 1/6-1/3 of the height of the culture bottle. The number and the arrangement of culture bottles need to be set according to actual conditions, and the culture bottles are 8 in the embodiment and are arranged in two rows.

The square box body 4 is provided with a box body handle 3, and the box cover 2 is provided with a box cover handle 1. The box body handle 3 is arranged to facilitate the movement of the combined tissue culture device, and the box cover handle 1 is arranged to facilitate the lifting and putting down of the culture bottle through the box cover.

Go up and be provided with fixed bulge 7 on the bottle, the box body on be provided with fixed recess, the blake bottle generally is the glass material, the surface is smooth, though they extrude each other closely, the lid can promote them simultaneously, but has the risk of landing, sets up fixed bulge 7, and fixed recess phase-match on the lid makes it fixed firm, does not have the risk of landing.

In order to further improve the sealing performance between the box body and the culture bottle, the sealing layer 8 is arranged on the inner edge of the box cover, so that the box cover and the square box body 4 can be formed. The contact of the culture bottle is more compact, and the sealing performance is improved.

The blake bottle adopts the glass bottle in this embodiment, and other high temperature resistance blake bottle materials are adopted to square box body 4 and lid, like polystyrene etc.

The operation process of the embodiment:

preparing a culture medium, then providing a box cover handle 1, lifting the whole culture bottle, pouring the well-matched culture medium into a square box body 4, wrapping a sealing film on the culture bottle, and putting the culture bottle into a sterilization mechanism for sterilization operation. The culture medium is in the square box 4 during the sterilization process, and the culture medium is always in a high-temperature state, so that the culture medium and the square box can be uniformly mixed. After the sterilization is finished, the culture bottles are uniformly pushed downwards through the box cover handle 1 while the culture bottles are hot, so that the culture bottles are in close contact with the bottom of the square box body 4, the liquid culture medium uniformly enters each culture bottle, and the culture medium is uniformly subpackaged.

The device simple structure of this embodiment can realize the even quick partial shipment of culture medium, the work effect that improves effectively, the precision of the experiment of improvement.

Example 2

As shown in fig. 6 to 9, basically the same as in embodiment 1, except that: this example is exemplified by a square culture flask. The blake bottle includes square bottle 6 and the last bottle under bottomless, goes up the bottle and is the same with square bottle 6 shape down, for square bottle 10 on, adopts square bottle, can avoid effectively training in-process reflection of light problem of shooing, can improve the quality of shooing effectively. The square bottles are adopted, and if all the square bottles are in close contact, poor lighting in the plant cultivation process can be caused. Therefore, the combined tissue culture device in this embodiment further includes a light-transmitting grid 9, the light-transmitting grid 9 is provided with a clamping hole, the light-transmitting grid 9 is disposed between the lower square bottles 6, and the height of the light-transmitting grid is consistent with the height of the lower square bottles 6. The culture bottles are mutually separated through the transparent grating grids 9, so that the illumination can be irradiated from the periphery of the culture bottles like the culture bottles, and the plant tissues are ensured to have sufficient illumination.

In addition, the combined tissue culture device also comprises a lock catch, wherein a fixed part 11 of the lock catch is arranged on the box cover, and a movable part 12 of the lock catch is arranged on the square box body 4. The square box body 4 and the box cover can be tightly fixed together after sterilization by adopting the lock catch, so that the antibacterial effect of the device is further improved.

Example 3

As shown in fig. 10, basically the same as embodiment 2, except that: the combined tissue culture device also comprises a flexible box cover lining 13 and a flexible box body lining 14. The flexible box cover lining and the flexible box body lining are made of polyethylene materials or silica gel materials. When the device is used for sterilization, the square box body and the box cover are put into a sterilization device together for high-pressure steam sterilization. However, if the cartridge is too large, it is not placed in a sterilization apparatus for sterilization. Therefore, the utility model adopts the arrangement of the flexible box cover lining and the flexible box body lining. The flexible box cover lining and the flexible box body lining are made of polyethylene materials or silica gel materials. By adopting the device, only the culture bottle, the flexible box cover lining, the flexible box body lining, the transparent grating grid and the culture medium need to be sterilized by high-pressure steam during high-pressure steam sterilization. The flexible box cover lining and the flexible box body lining are foldable, and the light-transmitting grids can be detached, so that the sterilization space can be effectively reduced. The square box body and the box cover are sterilized by ultraviolet rays. After autoclaving, set up flexible box body inside lining in square box body inner wall in the heat, will pass through grating check and blake bottle and assemble in square box body, then sheathe flexible lid inside lining and lid in, pour the culture medium after the sterilization into square box body, utilize the lid handle, promote the lid, drive the downward motion of grating check and blake bottle, until contacting flexible box body inside lining, accomplish the partial shipment of culture medium. In this embodiment, adopt flexible lid inside lining and flexible box body inside lining, on the one hand can reduce the sterilization space effectively, on the other hand adopts flexible material, can improve the leakproofness of equipment effectively.

Although embodiments of the present invention have been described above, it would be appreciated by those skilled in the art that changes may be made in these embodiments without departing from the principles and spirit of the utility model, the scope of which is defined in the claims.

Claims (8)

1. A combined tissue culture device, characterized in that: including square box body, lid and a plurality of blake bottle, the blake bottle including the square lower bottle body and the last bottle body of bottomless, the lid on be provided with the card hole, the square lower bottle body of blake bottle install in square box body, and with the inner wall in close contact with of square box body, the last bottle body of blake bottle pass through the card hole with the lid install together, square box body and lid sealed together, square box body highly be 1/6-1/3 of blake bottle height.

2. The combined tissue culture device of claim 1, wherein: the square box body is provided with a box body handle, and the box cover is provided with a box cover handle.

3. The combined tissue culture device of claim 1, wherein: the upper bottle body is provided with a fixing bulge, and the box body is provided with a fixing groove.

4. The combined tissue culture device of claim 1, wherein: the inner edge of the box cover is provided with a sealing layer.

5. The combined tissue culture device of claim 1, wherein: the combined tissue culture device also comprises a lock catch, wherein the fixed part of the lock catch is arranged on the box cover, and the movable part of the lock catch is arranged on the square box body.

6. A combined tissue culture device according to any one of claims 1 to 5, wherein: the combined tissue culture device further comprises a light transmission grating, wherein clamping holes are formed in the light transmission grating, the light transmission grating is arranged between the square lower bottle bodies, and the height of the light transmission grating is consistent with that of the square lower bottle bodies.

7. The combined tissue culture device of claim 1, wherein: the combined tissue culture device also comprises a flexible box cover lining and a flexible box body lining.

8. The combined tissue culture device of claim 7, wherein: the flexible box cover lining and the flexible box body lining are made of polyethylene materials or silica gel materials.

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