KR101385939B1 - Photobioreactors for microalgal mass cultures and cultivation methods using them - Google Patents

Photobioreactors for microalgal mass cultures and cultivation methods using them Download PDF

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KR101385939B1
KR101385939B1 KR1020100076416A KR20100076416A KR101385939B1 KR 101385939 B1 KR101385939 B1 KR 101385939B1 KR 1020100076416 A KR1020100076416 A KR 1020100076416A KR 20100076416 A KR20100076416 A KR 20100076416A KR 101385939 B1 KR101385939 B1 KR 101385939B1
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microalgae
film
membrane
light
photobioreactor
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KR20120014387A (en
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이철균
임상민
배재한
조광국
김지훈
강신애
김혜정
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인하대학교 산학협력단
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Priority to KR1020100076416A priority Critical patent/KR101385939B1/en
Priority to PCT/KR2011/001147 priority patent/WO2011102693A2/en
Priority to US13/580,635 priority patent/US20130052719A1/en
Priority to AU2011216650A priority patent/AU2011216650A1/en
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/56—Floating elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • C12M41/10—Filtering the incident radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

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Abstract

According to an aspect of the present invention, in the buoyancy type optical bioreactor consisting of a culture vessel made of a light-transmissive material and a support means fixed thereto, the culture vessel is a reaction for receiving the microalgae in the inner space defined by the outer wall room; A first film forming part of the outer wall; And a second membrane which forms a part of the outer wall at a position different from the first membrane, and which has a property different from the first membrane.

Description

Photobioreactors for microalgal mass cultures and cultivation methods using them

The present invention relates to an optical bioreactor supported by seawater or fresh water and capable of cultivating a large amount of microalgae, and more particularly, to an optical bioreactor having an outer wall formed by using a plurality of membranes having different characteristics.

Microalgae, which are photosynthetic single cell microorganisms, can produce various organic materials such as proteins, carbohydrates, and fats through photosynthesis. In recent years, not only the production of high value products such as functional polysaccharides, carotenoids, vitamins and unsaturated fatty acids, but also the main cause of global warming has been evaluated as an optimum organism for the purpose of removing carbon dioxide. The main reason is that the doubling time for the effective removal of carbon dioxide, a major culprit of global warming in terms of quantity, is shorter than that of land plants, and shows high growth potential even in a harsh environment, and direct combustion gas from a power plant or plant. Because it can be used as.

In connection with the removal of carbon dioxide, it is receiving great interest in the production of biological energy to replace fossil fuels, which are finite energy sources. This is due to the ability of microalgae to store carbon dioxide and accumulate lipids in living organisms. Much research has been conducted on the production of biodiesel using the accumulated lipids.

However, in order to mass-produce useful products such as the removal of carbon dioxide using microalgae or the production of bioenergy, microalgae cultivation must be carried out at a large scale and at a high concentration. Therefore, the technology related to the construction of large-scale cultivation facilities is essential.

Conventionally, various types of photobioreactors installed indoors are used as a culture facility for culturing microalgae. Most of the conventional photobioreactors were made of glass such as expensive pyrex or a material using the same, and had to have artificial lighting means. Therefore, a large amount of capital must be invested for production, and a lot of money is required for maintenance and operation even after production.

In addition, it is not easy to scale up or manufacture a unit reactor on land, and it is impossible to scale up infinitely due to the reduction of light energy due to the pigment of microalgae.

Therefore, for commercial mass cultivation, securing economic feasibility is the most important prerequisite, and therefore, it is urgently required to develop a cultivation technology that is capable of cultivating a high concentration at low cost and easy to scale up.

The present invention is an optical bioreactor supported by seawater or fresh water and capable of cultivating a large amount of microalgae contained therein, and having a plurality of membranes having different characteristics to improve the culture efficiency of microalgae. For the purpose of providing

The object of the present invention is not limited to those mentioned above, and other objects not mentioned will be clearly understood by those skilled in the art from the following description.

According to one aspect of the present invention for solving the above problems, in a flotation type optical bioreactor comprising a culture vessel made of a light-transmitting material and a support means fixed thereto, the culture vessel is in an inner space defined by an outer wall A reaction chamber accommodating the microalgae; A first film forming part of the outer wall; And a second membrane which forms a part of the outer wall at a position different from the first membrane, and which has a property different from the first membrane.

In this case, the first membrane and the second membrane may have different light transmission characteristics, optical filter characteristics, or material transmission characteristics.

In addition, the first film and the second film may have different light reflection characteristics, and the second film may reflect the light wavelength transmitted through the first film to be supplied to the reaction chamber.

According to another aspect of the invention, the step of injecting the culture medium into the culture vessel of the above-described photobiological incubator and inoculating microalgae; Sealing the culture vessel and fixing the flotation means, and then putting the culture vessel into marine or fresh water; And it provides a microalgae culturing method using a flotation type photobiological reactor comprising the step of allowing the microalgae to perform photosynthesis by sunlight.

The flotation type photobioreactor according to the present invention may be formed by combining membranes having different optical and material transmission characteristics, thereby maximizing culture efficiency in the photobioreactor. Therefore, when the photobioreactor is installed in the sea, it is freed from spatial constraints, thereby enabling horizontal scale-up and easy and economical mass culture of microalgae.

The effects of the present invention are not limited to those mentioned above, and other effects not mentioned can be clearly understood by those skilled in the art from the following description.

Figure 1 shows a flat type photobiological reactor as an embodiment of the photobiological reactor according to the present invention.
2 (a) to 2 (c) illustrate the shape of a light blocking pattern for changing light transmittance.
3 illustrates an optical bioreactor having a reflective member in a lower film.
4 and 5 illustrate cylindrical and elliptical photobioreactors, respectively, as photobioreactors in accordance with one embodiment of the present invention.
FIG. 6 illustrates that the plate-shaped photobioreactor of FIG. 1 is manufactured in a cluster form.
FIG. 7 illustrates that the cylindrical photobioreactor of FIG. 3 is manufactured in the form of a cluster.

Hereinafter, with reference to the accompanying drawings will be described in detail a preferred embodiment of the present invention. In the following description of the present invention, detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

 The culture vessel of the photobioreactor according to the present invention comprises a reaction chamber having an outer wall having a predetermined thickness and an inner space in which microalgae and a medium are accommodated. The outer wall may include a plurality of films having different film characteristics.

For example, the outer wall may include a plurality of films having different optical properties such as light transmittance (or light blocking rate), light transmittance characteristics (optical filter characteristics), and reflectance according to a wavelength region.

As another example, the outer wall may include a plurality of membranes having different transmittances according to materials.

Figure 1 shows a perspective view of a flat plate bioreactor 100 according to an embodiment of the present invention. Referring to FIG. 1, the planar photobioreactor 100 has a rectangular parallelepiped shape. In this case, the outer wall has different characteristics and includes different membranes spaced up and down.

In the first embodiment, the first film 2 having a relatively low light transmittance may form an upper film of the rectangular parallelepiped, and the second film 3 having a higher light transmittance than the first film 2 may form a lower film. At this time, the inner space defined by the outer wall including the first membrane 2 and the second membrane 3 becomes the reaction chamber 1 in which the microalgae and the medium are accommodated.

In this case, when the photobioreactor 100 floats in seawater or fresh water as shown in FIG. 1, the lower layer is in contact with the water or submerged to a certain depth below the surface, and the upper layer is in contact with the atmosphere and is primarily exposed to the light source. do.

In FIG. 1, the first layer 2 forms the upper layer and the second layer 3 forms the lower layer, but the present invention is not limited thereto. When the photobioreactor 100 is inverted, the first layer 2 may be inverted. The lower layer and the second layer 3 may form the upper layer.

In this case, the photobioreactor 100 may further include a gas inlet 4 capable of supplying gas into the reaction chamber 1 and a gas outlet 5 for discharging the gas.

In addition, it may be further provided with a sampling port 6 for taking a sample for confirming the degree of culture of the microalgae.

In this case, the microalgae may include chlorella, haematococcus, botryococcus, senedmus, nannoclopsis, nannochloris, spirulina, chlamidomonas, phadocallium, dunaliella, kizokaitrium, and nizchia. . In this case, the microalgae may produce carotenoids, cells, phycobiliproteins, lipids, carbohydrates, unsaturated fatty acids, and proteins in the photobioreactor.

In addition, the microalgae may be microalgae for producing biodiesel, which is an important energy source for the industry. Microalgae can also remove carbon dioxide contained in the atmosphere.

According to the photobioreactor 100 according to an embodiment of the present invention, the light energy input to the reaction chamber 1 is timed using the first membrane 2 and the second membrane 3 having different light transmittances. Can be adjusted by star or step. In particular, when the photobiological incubator 100 according to an embodiment of the present invention is suspended in seawater or fresh water and exposed to sunlight, the amount or intensity of sunlight that is selectively introduced into the reaction chamber 1 in response to the amount of solar radiation. Can be adjusted.

That is, in order to efficiently cultivate the microalgae, it is necessary to effectively apply the microalgae production method in consideration of the brightness according to the position of the sun. Seasons change with the Earth's orbit, and consequently, the extent to which solar energy reaches the earth's surface. In addition, night and day exist according to the rotation of the earth, and during daytime, the degree of solar energy arrival varies according to the rotation cycle of the earth.

When microalgae use light energy generated from the sun, the microalgae are destroyed when the photosynthetic device receives the light energy above a certain level of light, and thus photosynthesis is no longer possible or the secondary metabolites are accumulated to overcome high light energy. If the purpose is cell production, it may produce unwanted products.

In addition, depending on the microalgae, the light energy required for cultivation may have a different range, or even in one microalgae, the light energy required for each step in the culturing process may be different. As an example, the induction production process of astaxanthin in Haematococcus may be performed in two steps. That is, in the first stage of cultivation, astaxanthin is induced with high light intensity in the second stage after sufficient production of cells by supplying relatively low light energy.

In the case of the photobioreactor 101 according to the embodiment of the present invention, an optimal brightness condition for culturing microalgae may be realized by appropriately selecting a surface exposed to sunlight when suspended in an ocean or a lake.

For example, during the high light intensity period or season, the first surface 2 having a low light transmittance is disposed on the upper portion so as to be exposed to the sunlight, thereby appropriately reducing the solar energy supplied to the reaction chamber 1, thereby deteriorating the light. It is possible to prevent the phenomenon and to supply the light energy appropriate to the cell.

On the other hand, during the time or season when the solar light intensity is relatively low, the photobioreactor 100 is reversed to expose the second membrane 3 having high transmittance to sunlight through the positional change of the upper and lower membranes, thereby being suitable for microalgae growth. Efficient cultivation of microalgae through light energy supply is possible.

In this case, as an example, the plurality of films having different light transmittances from each other may be made of a material having different light transmittances from each other. Or it can comprise by attaching the material which has a different light transmittance, for example, a light shielding film or a tape, on a light transmissive film.

 As another example, a plurality of films having different light transmittances may be configured by forming various types of light blocking patterns capable of controlling light transmittances on the light transmissive layers.

2 (a) to 2 (c) illustrate the form of a light blocking pattern (dark portion) that may be formed on one surface of the photobioreactor 100 in order to adjust the light transmittance.

FIG. 2A illustrates an example in which a pattern for blocking sunlight is not formed, and FIG. 2C illustrates an example in which sunlight is completely blocked. In addition, Figure 2 (b) is made to block the solar light a certain ratio, for example, 30, 50, 70%.

The light blocking pattern may itself have a property of not transmitting light or only a portion of light.

In addition, the light blocking pattern may be formed on the film material itself or by attaching a light blocking film or tape to the light transmitting film.

Although one embodiment of the present invention has been described with reference to the above-described solar light, the present invention is not limited thereto, and it is obvious that the present invention can be applied to a light source other than sunlight, for example, an LED lamp. The same applies to the following examples.

On the other hand, in the case of the optical wavelength which is one of the important optical factors in culturing the microalgae, it is possible to improve the concentration of the microbial cells and the concentration of the metabolite produced from the microalgae by supplying the optical wavelength of the specific wavelength region to the microalgae.

Microalgae have chlorophyll and various pigments for photosynthesis. In general, the wavelength used for photosynthesis is 300 to 700 nm, the green algae mainly absorb the light wavelength of the red or blue region is used for photosynthesis.

For example, the species, one of the green alga Chlorella (Chlorella) is when supplying a light wavelength energy of the red area by using the red light-emitting diode (680nm), will supply the mixed light and blue light, a short wavelength of the light energy of the green- Haematococcus had a higher growth rate, and in the case of Haematococcus , the production concentration of astaxanthin, a kind of carotenoid with excellent antioxidant power, was produced by culturing using any light wavelength or region.

In addition, growth of microalgae is known to be inhibited when exposed to ultraviolet light for a long time.

Therefore, in order to increase the growth of the microalgae and to remove wavelengths unnecessary for growth, it may be efficient to selectively irradiate only a specific wavelength region.

For example, the production of expensive carotenoids such as beta-carotene, lutein, and astaxanthin from microalgae increases the productivity when only a specific wavelength is supplied. Can be obtained.

Accordingly, as a second embodiment of the photobioreactor according to the present invention, an optical bioreactor including a plurality of membranes having different optical filter properties may be provided. That is, wavelength ranges that can be transmitted in the optical wavelength may have different ranges between the plurality of films.

 Referring to FIG. 1, the first film 2 and the second film 3 of the photobioreactor 100 may be manufactured to transmit light wavelengths of different wavelength regions.

When the photobioreactor 100 according to the present embodiment is suspended in the sea as shown in FIG. 1, the first film 2 having the optical filter function is exposed to a light source, for example, sunlight, to the first film 2. Only the wavelengths of some regions of the supplied sunlight can selectively transmit or block. Therefore, it is possible to intensively supply the wavelength of the specific region to the microalgae contained in the reaction chamber (1).

In addition, when it is necessary to supply the wavelength of the other region to the microalgae, the photobiological reactor 100 is turned upside down, and the upper and lower positions thereof are changed so that the second film 3 is exposed to the light source as the upper film, thereby obtaining the desired effect.

In this case, the above-described 'wavelength region' may be classified into, for example, a blue series, a red series, or a green series in the solar wavelength.

 In this case, the wavelength region to be transmitted or blocked may be appropriately selected depending on the type of microalgae to be cultured.

The film having such an optical filter function may be produced by mixing a chemical component capable of absorbing a light wavelength in a specific wavelength region in a plastic or polymer material. These chemical components may be included in the pigment pigments, Table 1 shows the available chemical components according to the pigment pigments.

Pigment Type Chemical composition by color Yellow pigment Lead Chromate (PbCrO 4 ), Yellow Iron Oxide (FeO (OH) or Fe 2 O 3 OH H 2 O), Cadmium Yellow (CdS or CdS + ZnS), Titanium Yellow (TiO 2 ㅇ NiO ㅇ Sb 2 O 3 ) Orange pigment Chromium Orange (PbCrO 4 ㅇ PbO), Molybdenum Orange (PbCrO 4 ㅇ PbMoO 4 ㅇ PbSO 4 ) Red pigment Red Iron Oxide (Fe 2 O 3 ), Photo Name (Pb 3 O 4 ), Cadmium Red (CdS + CdSe) Purple pigment Manganese Violet (NH 4 MnP 2 O 7 ) Blue pigment Royal Blue (Fe (NH 4 ) Fe (CN) 6 ㅇ xH 2 O), Navy Blue (Na 8-10 Al 6 Si 6 O 24 S 2-4 ), Cobalt Blue (CoO ㅇ Al 2 O 3 ) Green pigment Chromium Green (Lead Chromate + Eavesdropping), Emerald Green (Cu (CH 3 CO 2 ) 2 Cu (AsO 2 ) 2 )

Optionally, the first film 2 may be a coating material containing a pigment as shown in Table 1 on the light transmitting film, or a film or tape for an optical filter adhered thereto. The optical filter film or tape is an optical film or tape designed to transmit or block only a specific wavelength region.

In this case, the present embodiment can also form a plurality of films having different optical filter characteristics by forming an optical filter pattern having various shapes as illustrated in FIGS. 2A to 2C on the light transmissive film.

As a third embodiment of the photobioreactor 100 according to the present invention, the outer wall may be formed of a plurality of films having different light reflectances.

As an example, as shown in FIG. 3, the first film 2 is located at the top, the second film 3 is located at the bottom, and the second film 3 is a light wavelength transmitted through the first film 2. It may have a property of reflecting again.

Due to the reflection by the second film 3, the light wavelength that reaches the second film 3 without being supplied to the microalgae contained in the reaction chamber 1 among the light wavelengths transmitted through the first film 2 is again reacted. It is reflected by (1) and supplied to microalgae. Therefore, the supply rate finally supplied to the microalgae among the optical wavelengths transmitted through the first membrane 2 can be improved.

In this case, the second film 3 may be manufactured by stacking a reflective member 8 having a high reflectance on a portion of the light transmissive film. In this case, the reflective member may be a reflective film having a high reflectance on one surface, or a reflective tape.

In this case, the reflective member 8 may diffuse the reflected light wavelengths and evenly resupply the reflected light wavelengths in the internal space 1. To this end, irregularities for diffuse reflection may be further formed on the surface of the reflective member 1.

As a fourth embodiment of the photobioreactor 100 according to the present invention, the outer wall may be constituted by a plurality of membranes which may select different materials from each other.

For example, the first membrane 2 in contact with the atmosphere of the photobioreactor 100 of FIG. 1 is formed of a semi-permeable membrane capable of flowing in and out of the outside atmosphere and oxygen or carbon dioxide, and is in contact with seawater or seawater. Alternatively, the second membrane 3 submerged in fresh water may be formed as a semi-permeable membrane that allows the inflow and outflow of water and nutrients from the seawater, but blocks the outflow and outflow of microalgae.

Therefore, when the microalgae accommodated in the photobioreactor 100 made of such a semi-permeable membrane is suspended in seawater or fresh water, it is possible to naturally supply materials necessary for cultivation from the external environment in a state separated from the external environment.

For example, atmospheric carbon dioxide may be introduced into the first membrane 2 positioned above the water surface during suspension, and the carbon dioxide may be removed by photosynthesis of microalgae contained in the reaction chamber 1. In addition, oxygen formed by photosynthesis is discharged to the atmosphere through the first film (2).

On the other hand, through the second membrane (3) in contact with sea water or fresh water may be able to flow in and out of the nutrients, such as to the sea water or fresh water from the outside. In addition, the excreta discharged during the growth of microalgae and metabolites that interfere with the growth can be removed naturally when the seawater or freshwater is discharged to the outside by melting in seawater or freshwater. Therefore, no purification or medium replacement is required.

The outer wall of the photobioreactor according to the present invention is made of a light transmissive material, glass, plastic or polymer material, a semi-permeable membrane may be used.

On the other hand, the shape of the photobioreactor can be produced in various forms, it can be selected from the group consisting of a rectangular flat plate (cylinder) reactor, but is infinite in a large space that is easy to expand, such as the ocean Any shape can be used as long as it can apply solar energy, which is the energy of nature, to be customized for microalgae growth.

4 and 5 illustrate cylindrical and elliptical forms of this photobioreactor.

On the other hand, such a photobioreactor may be supported by itself, but may further include a fixing device that can be fixed in a certain range of the lifting means and the support position for floating in some cases.

For example, the upper portion of the photobioreactor may be provided with a support means for supporting the seawater or fresh water, the lower portion may be provided with a fixing device for fixing the photobioreactor.

In addition, this photobioreactor is suitable for the production of all useful products that can be produced from photosynthetic microorganisms, especially for the production of bioenergy (biodiesel, bioethanol, hydrogen gas) using microalgae, It is also applicable to carbon dioxide removal. In addition, the application is appropriate depending on the climate, marine or fresh water environment of the reactor installation area.

For mass culture, it is possible to connect a plurality of photobioreactors of all the above-described embodiments and install them in the form of a community.

6 and 7 illustrate an example in which a group of flat photobioreactors and a cylindrical photobioreactor is formed by collecting the cluster type.

 The foregoing description of specific embodiments of the invention has been presented for purposes of illustration and description. It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined by the appended claims. Do.

100 flotation type photobioreactor 1st membrane
2: 2nd membrane 3: reaction chamber
4 gas inlet 5 gas outlet
6: sampling port

Claims (8)

In the flotation type optical bioreactor comprising a culture vessel made of a light-transmitting material and a support means fixed thereto,
The culture vessel is a reaction chamber for accommodating the microalgae in the inner space defined by the outer wall;
A first film forming part of the outer wall and having a low light transmittance; And
A second film which forms a part of the outer wall at a position different from the first film and has a higher light transmittance than the first film
/ RTI >
The second film includes a reflecting member having a high light reflectivity, and the reflecting member again returns the light wavelength reaching the second film without being supplied to the microalgae accommodated in the reaction chamber among the light wavelengths passing through the first film. Reflected to the reaction chamber and supplied,
The culture vessel is inverted, the up-and-down position of the first membrane and the second membrane can be changed, the photobiological reactor. delete delete delete The photobioreactor of claim 1, wherein the first membrane further comprises a semipermeable membrane having selective permeability to oxygen or carbon dioxide, and the second membrane further comprises a semipermeable membrane having selective permeability to water and nutrients. The photobioreactor of claim 1, wherein the first membrane is at the top and the second membrane is at the bottom. The photobioreactor of claim 1, further comprising a gas inlet for supplying gas into the reaction chamber and a gas outlet for discharging the gas. Injecting the culture medium in the culture vessel of any one of claims 1 and 5 to 7 and inoculating microalgae;
Sealing the culture vessel and fixing the flotation means, and then putting the culture vessel into marine or fresh water; And
Microalgae culture method using a flotation type photobiological reactor comprising the step of allowing the microalgae to perform photosynthesis by sunlight.


KR1020100076416A 2010-02-22 2010-08-09 Photobioreactors for microalgal mass cultures and cultivation methods using them KR101385939B1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
KR1020100076416A KR101385939B1 (en) 2010-08-09 2010-08-09 Photobioreactors for microalgal mass cultures and cultivation methods using them
PCT/KR2011/001147 WO2011102693A2 (en) 2010-02-22 2011-02-22 Photobioreactor for mass culture of microalgae, and method for culturing microalgae by using same
US13/580,635 US20130052719A1 (en) 2010-02-22 2011-02-22 Photobioreactor for mass culture of microalgae, and method for culturing microalgae by using same
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