CN215454958U

CN215454958U - Carrier support for cryopreservation or thawing recovery of biological tissue

Info

Publication number: CN215454958U
Application number: CN202120458103.8U
Authority: CN
Inventors: 陈昊楠; 王东
Original assignee: Shenzhen Beierluoke Biotechnology Co ltd
Current assignee: Shenzhen Beierluoke Biotechnology Co ltd
Priority date: 2021-03-03
Filing date: 2021-03-03
Publication date: 2022-01-11
Anticipated expiration: 2031-03-03

Abstract

The utility model discloses a carrier support frame for cryopreservation or thawing and resuscitation of biological tissues, which is characterized by comprising the following components: the cell body, the cell body includes bottom and two lateral wall portions, the one end of bottom is provided with the multiunit fixed slot. The utility model can simultaneously bear a plurality of carriers, can ensure that the carriers can move together more conveniently, and fixes the direction of the carriers, thereby ensuring that the carriers can not displace in the freezing or thawing process, improving the stability and improving the efficiency.

Description

Carrier support for cryopreservation or thawing recovery of biological tissue

Technical Field

The utility model relates to the technical field of biological engineering, in particular to a carrier support frame for cryopreservation or thawing and resuscitation of biological tissues.

Background

Cell cryopreservation is an important technique in many biological fields, as is the restoration of cells from a cryo-frozen state to a normal metabolic state, and this thawing recovery process involves the removal of cryoprotectants and replacement with normal culture media.

In the prior art, a plurality of carriers are needed to be used when the embryo is frozen or thawed, and a plurality of operations are needed to be carried out on the plurality of carriers, so that the operation is complicated and the efficiency is low.

SUMMERY OF THE UTILITY MODEL

The utility model mainly aims to provide a carrier support frame to solve the problems of complex operation, low efficiency and the like when the carrier in the prior art is used for freezing or unfreezing embryos.

According to one aspect of the present invention, there is provided a carrier support for cryopreservation or thawing recovery of biological tissue, comprising: the cell body, the cell body includes bottom and two lateral wall portions, the one end of bottom is provided with the multiunit fixed slot.

Wherein, each group of fixed slots includes a plurality of buckles.

Wherein, the plurality of groups of buckles are arranged in a staggered manner.

Wherein a partial area of the bottom is provided with a hollow.

Wherein, one end of the bottom is provided with a notch.

Wherein, the bottom of the groove body is run through in the length direction.

Wherein, the cell body is provided with 3 groups of fixed slots.

According to another aspect of the present invention, there is also provided a carrier scaffold for cryopreservation or thawing recovery of biological tissue, comprising: the tank body comprises a bottom and two side wall parts, wherein one end of the bottom is provided with a plurality of groups of fixing grooves; a plurality of carriers carried on the bottom and secured by the plurality of sets of securing slots.

The fixing groove comprises a plurality of buckles, and the plurality of groups of buckles are arranged in a staggered mode.

Wherein a partial area of the bottom is provided with a hollow.

Wherein, one end of the bottom is provided with a notch.

Wherein, the bottom of the groove body is run through in the length direction.

Wherein, the cell body is provided with 3 groups of fixed slots, the quantity of carrier is 3.

According to the technical scheme of the utility model, the carrier support frame can simultaneously bear a plurality of carriers, so that the carriers can move together more conveniently, the direction of the carriers is fixed, the carriers are ensured not to displace in the freezing or thawing process, the stability is improved, and the efficiency is improved.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the utility model and together with the description serve to explain the utility model without limiting the utility model. In the drawings:

FIG. 1 is a perspective view of a carrier support according to one embodiment of the present invention;

FIG. 2 is a top view of a carrier support according to one embodiment of the present invention;

FIG. 3 is a cross-sectional view taken along line A-A of FIG. 2;

FIG. 4 is a perspective view of a carrier support according to another embodiment of the present invention;

FIG. 5 is a top view of a carrier support according to another embodiment of the present invention;

fig. 6 is a bottom view of a carrier support according to another embodiment of the present invention;

fig. 7 is a cross-sectional view taken along line B-B of fig. 5.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the specific embodiments of the present invention and the accompanying drawings. It is to be understood that the described embodiments are merely exemplary of the utility model, and not restrictive of the full scope of the utility model. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In cell cryopreservation, a carrier is typically used to carry the embryos/ova. Only 1 to 2 embryos will typically be placed on a single carrier, and a patient will typically have multiple embryos to be cryopreserved, which results in the need for multiple carriers for the same patient. In the case of manual freezing procedures, the embryologist has to place multiple embryos on multiple carriers after processing multiple embryos on a single patient with cryoprotectants. Problems arise during handling due to the narrow (2.5 mm) and long support itself. For example, the carriers are not oriented, embryos are placed on one carrier and then the other carrier is not in the field of view of the microscope, multiple carriers are difficult to move together, carriers tend to tumble when moving, and the like.

In an automated freezing and thawing system, a single chip can handle multiple carriers. However, when the chip and the carrier move relatively, the plurality of carriers may be displaced, which affects the freezing and thawing effect; moreover, when multiple embryos on multiple carriers are treated with cryoprotectants of an automated system at the same time, liquid nitrogen cannot be introduced simultaneously and simply in a short time, causing excessive toxic damage.

The technical solutions provided by the embodiments of the present invention are described in detail below with reference to the accompanying drawings.

According to the embodiment of the utility model, the carrier support frame is applied to the cryopreservation or thawing and reviving process of the biological tissue, and particularly can carry a carrier containing the biological tissue. The biological tissue may be a biological material such as an embryo, an ovum, and a cell, which is not limited in the present application.

Referring to fig. 1 to 3, the carrier support frame is substantially a flat structure, and includes a bottom 11 and two side walls 12 connected to the bottom 11, the bottom 11 and the two side walls 12 form a trough, and the bottom 11 is through in a length direction, i.e. the bottom 11 is open in the length direction.

As shown, one end of the bottom 11 is provided with a plurality of sets of fixing grooves 15, which can be used for fixing a plurality of sets of carriers, and the number of the fixing grooves can be 2, 3, 4 or 5 sets. The number of the fixing grooves is corresponding to the number of the fixed carriers, for example, when 3 carriers need to be fixed, 3 groups of fixing grooves need to be arranged on the bottom 11. Wherein, each group of fixing grooves may include one or more pairs of buckles or elastic buckles, and the plurality of pairs of buckles may be arranged in a staggered manner due to the limited area of the bottom 11. In the embodiment shown in fig. 2, the housing includes 3 sets of

retaining grooves

21, 22, 23, the

retaining grooves

21 and 23 on the left and right sides include 2 pairs of snaps (i.e., a first row and a third row of snaps), respectively, and the retaining groove 22 in the middle includes 1 pair of snaps (a second row of snaps). It should be noted that the solution of the present application for fixing the carrier is not limited thereto, and other structures capable of fixing the carrier are also within the scope of the present application.

In the present embodiment, a part of the area of the bottom 11 is provided with a hollow 16, which is convenient for the user to manipulate the sleeve of the carrier through the hollow 16. And, one end of the bottom is provided with a notch 17, and the notch 17 can be used for butting with another sleeve (different from the sleeve of the carrier) and fixing the position of the carrier support in the other sleeve.

There is also provided in accordance with an embodiment of the present application a carrier scaffold for cryopreservation or thawing resuscitation of biological tissue, the carrier scaffold including, with reference to fig. 4 to 7: a trough body and a plurality of carriers, wherein the structure of the trough body refers to the embodiment shown in fig. 1 to 3, and the detailed description is omitted here. The carrier comprises a front sheet 31, a rear carrying rod 32 and a sleeve 33 sleeved on the carrying rod, wherein the front sheet 31 is provided with a groove for accommodating embryos, the rear carrying rod 32 is fixed by a fixing groove, and the sleeve 33 can slide back and forth on the carrying rod to cover or expose the front sheet 31.

It should be noted that in the embodiments of fig. 4 to 7, 3 carriers are carried on the bottom of the carrier support frame and are fixed by 3 sets of fixing grooves, but the number of the fixing grooves and the carriers may be other numbers, but the present application does not limit the number. In addition, the number of carriers to be fixed may be less than the number of fixing grooves, for example, 1 carrier or 2 carriers may be fixed to a housing having 3 sets of fixing grooves.

After the carriers are placed on the carrier support frame, the carriers are close to each other, and a plurality of carriers can be observed under the visual field of the same microscope. The freezing or thawing operation using the carrier support is described in detail below.

1. The carrier support is used for freezing or thawing by hand.

After the embryos are treated by the vitrification refrigerating fluid according to the normal freezing operation, an operator can respectively place a plurality of embryos on the carriers of the carrier supporting frame. Because the carrier support frame is a flat structure, an operator can easily adjust or press and fix the position of the whole set of carriers in the visual field range of the microscope at the same time, so that the operator can simply distribute a plurality of embryos onto a plurality of carriers. After the embryos are all placed on the carriers, liquid nitrogen can be simultaneously added into the 3 carriers, so that the treatment efficiency is increased. At this time, if the carrier is designed as a conventional sleeve, the sleeve can be inserted onto the carrier using tweezers to protect the front transparent sheet portion. How to use the sleeve design of pushing from back to front for the carrier, so the operator just can hold carrier support frame in one hand, and 3 sleeves (because the back of carrier support frame is hollow design, embryologist can clip the sleeve around with the hand) are held in the other hand and are pushed down simultaneously, just can let the sleeve block in required position, accomplish the action of complete set sleeve.

When thawing is required, the operator can perform the thawing operation according to a planned thawing plan, such as 3 vectors thawed at the same time, 2 vectors thawed only, or one vector thawed only. If only one carrier needs to be thawed, the desired carrier can be removed from the carrier support frame and then thawed according to conventional manual thawing methods. If 2 carriers need to be thawed, the carrier that does not need to be thawed can be removed from the carrier support frame and returned to the liquid nitrogen holding device. The embryologist can then hold the carrier holder, insert 2 carriers simultaneously into the thawing solution for thawing and rewarming, and then perform the conventional manual thawing operation. Similarly, if 3 carriers need to be thawed at the same time, the carrier support frame is directly taken out from the liquid nitrogen, an embryologist holds the carrier support frame and inserts the carrier support frame and the 3 carriers into the thawing solution together for thawing and rewarming, and then the routine manual thawing operation is carried out.

2. Freezing or thawing the carrier support frame in an automated manner.

First, the operator places the embryos to be cryopreserved from the culture dish into the grooves on the carriers of the carrier support rack, respectively. Because the carrier support is a flat structure, it is easy for an embryologist to adjust or hold the position of the entire set of carriers in the field of view of the microscope at the same time, allowing the embryologist to simply distribute multiple embryos onto multiple carriers. After the embryos are placed on the carriers, the carriers along with the carrier support frame can be placed on a machine for freezing. In the process of freezing treatment, the carrier support frame liquid plays a role in fixing the carrier position, and the phenomenon that the freezing effect is influenced by displacement generated in the relative motion of the chip and the carrier is avoided. After the machine treatment is completed, the embryologist can hold the carrier support frame, and the transparent sheet parts of the 3 carriers are immersed in liquid nitrogen, so that the embryos in the grooves are rapidly frozen and cooled. At this time, if the carrier is designed by using a traditional sleeve, the sleeve can be sleeved on the carrier by using tweezers to protect the transparent sheet part at the front end; if the carrier is designed to be a sleeve which is pushed from back to front, an embryologist can hold the carrier support frame by one hand and hold 3 sleeves by the other hand (because the back of the carrier support frame is of a hollow design), the embryologist can clamp the sleeves by hands back and forth) and push the sleeves downwards at the same time, and then the sleeves can be clamped at the required positions to finish the action of the sleeves. Finally, the whole support frame and the carrier are put into liquid nitrogen by an embryologist to finish the cryopreservation operation.

When thawing is required, the embryologist can perform the thawing operation according to a planned thawing schedule, such as 3 vectors thawed simultaneously, 2 vectors thawed only, or one vector thawed. If only one carrier needs to be thawed, the required carrier can be taken down from the carrier support frame, the carrier is placed on the other (second) carrier support frame under the condition of ensuring that the transparent thin-sheet part is soaked in liquid nitrogen, then the whole carrier and the support frame are taken out from the liquid nitrogen, then the carrier sleeve is pulled out, the oil-phase thawing liquid is quickly invaded for thawing and rewarming, and after 3-5 seconds, the carrier is placed on an automatic thawing machine for thawing treatment. If 2 carriers need to be thawed, the carrier that does not need to be thawed can be removed from the carrier support frame and returned to the liquid nitrogen holding device. Then an embryologist can take the carrier support frame out of the liquid nitrogen, pull out the carrier sleeve, quickly invade the oil-phase thawing liquid to thaw and rewarm, and put the oil-phase thawing liquid on an automatic thawing machine for thawing treatment after 3 to 5 seconds. Similarly, if 3 are needed to be thawed simultaneously, the carrier support frame is directly taken out from the liquid nitrogen, the carrier sleeve is pulled out together, the oil-phase thawing liquid is rapidly invaded for thawing and rewarming, and after 3 to 5 seconds, the carrier support frame is put on an automatic thawing machine for thawing treatment, wherein the carrier support frame liquid plays a role in fixing the position of the carrier, so that the phenomenon that the thawing effect is influenced by displacement generated in the relative motion of the chip and the carrier is avoided. After the thawing process is completed, the embryologist can conveniently retrieve thawed and recovered embryos in the grooves of the plurality of carriers by using a glass capillary and place the embryos in a culture solution for further culture.

The above description is only an example of the present invention, and is not intended to limit the present invention, and it is obvious to those skilled in the art that various modifications and variations can be made in the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A carrier support for cryopreservation or thawing recovery of biological tissue, comprising: the cell body, the cell body includes bottom and two lateral wall portions, the one end of bottom is provided with the multiunit fixed slot.

2. The carrier support frame of claim 1 wherein each set of securing slots comprises a plurality of snaps.

3. The carrier support of claim 2 wherein the sets of snaps are staggered.

4. The carrier support of claim 1 wherein a partial area of the bottom portion is provided with a hollow.

5. The carrier support of claim 1 wherein one end of the base is provided with a notch.

6. The carrier support of claim 1 wherein the bottom of the channel extends through in the length direction.

7. The carrier support of any one of claims 1 to 6 wherein the trough is provided with 3 sets of securing slots.

8. A carrier support for cryopreservation or thawing recovery of biological tissue, comprising:

the tank body comprises a bottom and two side wall parts, wherein one end of the bottom is provided with a plurality of groups of fixing grooves;

a plurality of carriers carried on the bottom and secured by the plurality of sets of securing slots.

9. The carrier support frame of claim 8 wherein the retaining slots comprise a plurality of snaps, and wherein the sets of snaps are staggered.

10. The carrier support of claim 8 wherein a partial area of the bottom portion is provided with a hollow.

11. The carrier support of claim 8 wherein one end of the base is provided with a notch.

12. The carrier support of claim 8 wherein the bottom of the channel extends through in the length direction.

13. The carrier support of any one of claims 8 to 12 wherein the trough is provided with 3 sets of securing slots and the number of carriers is 3.