CN215560186U - Substrate and system for cryopreservation or thawing recovery of biological tissue - Google Patents
Substrate and system for cryopreservation or thawing recovery of biological tissue Download PDFInfo
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- CN215560186U CN215560186U CN202120458131.XU CN202120458131U CN215560186U CN 215560186 U CN215560186 U CN 215560186U CN 202120458131 U CN202120458131 U CN 202120458131U CN 215560186 U CN215560186 U CN 215560186U
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Abstract
The utility model discloses a substrate and a system for cryopreservation or thawing recovery of biological tissues, wherein the substrate comprises: the bearing device comprises a first bearing part, a second bearing part and a transition part; two sides of the bottom of the first bearing part are provided with first positioning plates; the second bearing part is provided with a track along the length direction of the second bearing part; the transition part is connected between the first bearing part and the second bearing part in an arc shape; and a second positioning plate is arranged at the position, close to the second bearing part, of the transition part. According to the utility model, when the biological tissue is subjected to cryopreservation or thawing recovery, the loaded devices can be effectively operated, and the method has the effects of simple operation and efficiency improvement.
Description
Technical Field
The utility model relates to the technical field of biological engineering, in particular to a substrate and a system for cryopreservation or thawing recovery of biological tissues.
Background
Cell cryopreservation is an important technology in many biological fields, and is also important for restoring cells from a cryo-frozen state to a normal metabolic state, and the thawing recovery process comprises the process of removing cryoprotectants and replacing the cryoprotectants with normal culture media.
The device used in the prior art for freezing or unfreezing the embryo has the problems of complex operation, low efficiency and the like.
SUMMERY OF THE UTILITY MODEL
The utility model mainly aims to provide a substrate and a system for cryopreservation or thawing recovery of biological tissues, so as to solve the problems of complex operation, low efficiency and the like when embryo freezing or thawing is carried out by using a carrier in the prior art.
According to an embodiment of the utility model, a substrate for cryopreservation or thawing recovery of biological tissue is proposed, comprising: the bearing device comprises a first bearing part, a second bearing part and a transition part; two sides of the bottom of the first bearing part are provided with first positioning plates; the second bearing part is provided with a track along the length direction of the second bearing part; the transition part is connected between the first bearing part and the second bearing part in an arc shape; and a second positioning plate is arranged at the position, close to the second bearing part, of the transition part.
Wherein the transition portion has a handle.
The second bearing part comprises a groove bottom and groove walls, and the rails are arranged on the left side and the right side of the groove bottom of the second bearing part.
The groove bottom of the second bearing part is provided with a thickness, and the groove bottom of the second bearing part is provided with a part or all of transparent bottom.
Wherein the height of the track is half of the height of the groove wall of the second bearing part.
Wherein the width of the second bearing part is larger than that of the first bearing part.
There is also provided, in accordance with an embodiment of the present invention, a system for cryopreservation or thawing resuscitation of biological tissue, including: a carrier support and a base; the carrier support frame is provided with a plurality of groups of fixing grooves, and a plurality of carriers for bearing embryos are fixed in the plurality of groups of fixing grooves; the substrate includes: the bearing device comprises a first bearing part, a second bearing part and a transition part; two sides of the bottom of the first bearing part are provided with first positioning plates; the second bearing part is provided with a track along the length direction of the second bearing part; the transition part is connected between the first bearing part and the second bearing part in an arc shape; a second positioning plate is arranged at the position, close to the second bearing part, of the transition part; wherein the first and second positioning plates define the position of the carrier support frame.
Wherein the width of the second bearing part is larger than that of the first bearing part.
Wherein the system further comprises: the carrier, the carrier is including carrying the pole body, carry the pole body including front end thin slice and rear end and carry the pole, the front end thin slice is provided with the recess that bears the embryo.
The carrier also comprises a sleeve sleeved on the carrier rod body.
Wherein the second carrier part comprises a trough bottom with a partially or completely transparent bottom supporting the front lamellae of the carrier, and trough walls.
Wherein the system further comprises: a chip comprising a plurality of compartments, the chip disposed over the track.
Wherein, the connection part of the first bearing part and the transition part defines the initial position of the chip placement.
According to the technical scheme of the utility model, the substrate capable of bearing the carrier support frame and the chip is provided, so that each borne device can be effectively operated when biological tissues are subjected to cryopreservation or thawing recovery, and the substrate has the effects of simple operation and efficiency improvement.
Drawings
The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the utility model and together with the description serve to explain the utility model without limiting the utility model. In the drawings:
FIG. 1 is a perspective view of a substrate according to an embodiment of the present invention;
FIG. 2 is a top view of a substrate according to an embodiment of the utility model;
FIG. 3 is a perspective view of a system according to one embodiment of the present invention;
FIG. 4 is a top view of a system according to one embodiment of the utility model;
FIG. 5 is a perspective view of a system according to another embodiment of the present invention;
fig. 6 is a top view of a system according to another embodiment of the utility model.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the specific embodiments of the present invention and the accompanying drawings. It is to be understood that the described embodiments are merely exemplary of the utility model, and not restrictive of the full scope of the utility model. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The technical solutions provided by the embodiments of the present invention are described in detail below with reference to the accompanying drawings.
There is provided, according to an embodiment of the utility model, a substrate for cryopreservation or thawing recovery of biological tissue, with reference to fig. 1 and 2, the substrate comprising: a first bearing part 11, a second bearing part 12 and a transition part 13.
The first bearing part 11 is substantially in a shape of a slot, and includes a slot bottom and two side walls. The first bearing part 11 has first positioning plates 111 on two sides of the bottom, the number of the first positioning plates 111 is 2, and the distance between the first positioning plates 111 is slightly larger than the width of the carrier support frame, that is, the first positioning plates 111 are used for positioning the left and right positions of the carrier support frame. In this embodiment, the first positioning plate 111 may be integrally formed with the first bearing part 11.
The second bearing part 12 is substantially in the shape of a slot, and the width of the second bearing part is larger than that of the first bearing part. The second bearing portion 12 includes a groove bottom and two side walls, the height of the groove bottom of the second bearing portion 12 is higher than that of the groove bottom of the first bearing portion 11, and the height of the side walls of the second bearing portion 12 is identical to that of the side walls of the first bearing portion 11. On the groove bottom of the second carrier part 12, there are 2 tracks 121 along the length direction of the second carrier part 12, and the tracks 121 are used for carrying chips.
The transition portion 13 is generally arcuate in shape and includes a slot bottom and two side walls. A transition portion 13 is connected between the first bearing portion 11 and the second bearing portion 12, and a second positioning plate 131 is disposed at a position of the transition portion close to the second bearing portion 12. The number of the second positioning plates 131 is 2, and the second positioning plates 131 can be used for positioning the front and rear positions of the carrier support frame. In the present embodiment, the transition portion 13 is further provided with 2 handles 132, and the handles 132 are respectively disposed on the side walls of the transition portion 13.
There is also provided, in accordance with an embodiment of the present application, a system for cryopreservation or thawing resuscitation of biological tissue, with reference to fig. 3 and 4, the system comprising: a substrate 1, a plurality of carriers 2 and a carrier support 3. The substrate 1 includes a first carrying portion, a second carrying portion and a transition portion, and the detailed structure of the substrate is please refer to the embodiment shown in fig. 1 and fig. 2, which is not described herein again.
Specifically, each carrier 2 comprises: carry the pole body and cover and establish carry sleeve 23 on the pole body, carry the pole body to include front end thin slice 21 and rear end and carry pole 22, front end thin slice 21 is provided with the first recess that bears the weight of the embryo. In addition, a second groove may be provided on the front sheet 21 at the rear end of the first groove.
The carrier support frame 3 is provided with a plurality of groups of fixing grooves, a plurality of carriers for bearing embryos are fixed on the plurality of groups of fixing grooves, and the length of the carrier 2 is greater than that of the carrier support frame 3. It should be noted that 3 carriers are fixed by the carrier support frame in this embodiment, and the number of carriers may be 1, 2, or 4 in other embodiments.
The base 1 has a first positioning plate 111 and a second positioning plate 131, the first positioning plate 111 and the second positioning plate 131 defining the position of the carrier support frame 3. Specifically, the first positioning plate 111 positions the carrier support frame 3 in the left-right direction, and the second positioning plate 131 positions the carrier support frame 3 in the front-rear direction, so that the carrier support frame 3 is fixed by the fixing base 1 (the first bearing portion and the transition portion). In this embodiment, the length of the carrier 1 exceeds the length of the carrier support 3, and the portion exceeding the carrier support 3 is the front sheet 21, and the transparent bottom of the second carrier portion of the substrate 1 supports the front sheet 21 of the carrier 1.
Referring to fig. 5 and 6, the system further includes a chip 4, four ends of the chip 4 are closed structures, the chip 4 includes a plurality of sections 41 and bonding portions 42 disposed on two sides of the plurality of sections, and the bonding portions 42 are disposed on the tracks of the substrate 1. Referring to the drawings, the connection of the first carrier portion and the transition portion of the base 1 defines an initial position in which the chip 4 is placed, that is, when cryopreservation or thawing recovery is performed, the chip 4 is initially placed in the position shown in fig. 5 and 6, and then the chip is pushed forward (in a direction toward the first carrier portion by the transition portion) to perform a freezing or thawing operation.
In practice, the carrier support and carrier are first placed on the base, the embryos on the culture dish are then removed from the incubator, and the base and dish are then placed on the stage of the microscope. The embryos are transferred from the petri dish to the wells of the carrier on the substrate using glass capillaries. Then the chip is placed above the structure that the substrate begins to narrow, the whole substrate is placed on the machine by holding the handle, and the frozen chip is pushed forwards according to the program after the machine is started, so that the frozen protective agent is delivered. After the delivery is finished, the user holds the part of the carrier support frame protruding out of the base by hand, extracts the carrier support frame and puts the carrier and the carrier into liquid nitrogen together for freezing. When unfreezing, the carrier support frame is taken out of the liquid nitrogen, the transparent sheet part of the carrier is quickly immersed into the unfreezing liquid, the support frame is placed on the base after 3-5 seconds, then the chip is placed above the structure where the base begins to narrow, the whole base is placed on the machine by holding the handle, the frozen chip can be pushed forwards according to the program after the machine is started, and therefore the frozen protective agent is removed. After removal, the whole substrate is removed from the machine by the embryologist, placed on the stage of the microscope, and the embryos are removed from the carrier wells using glass capillaries and transferred to the culture dish for further culture.
The above description is only an example of the present invention, and is not intended to limit the present invention, and it is obvious to those skilled in the art that various modifications and variations can be made in the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.
Claims (13)
1. A substrate for cryopreservation or thawing recovery of biological tissue, comprising: the bearing device comprises a first bearing part, a second bearing part and a transition part;
two sides of the bottom of the first bearing part are provided with first positioning plates;
the second bearing part is provided with a track along the length direction of the second bearing part;
the transition part is connected between the first bearing part and the second bearing part in an arc shape; and a second positioning plate is arranged at the position, close to the second bearing part, of the transition part.
2. The base of claim 1, wherein the transition portion has a handle.
3. The base of claim 1, wherein the second carrier includes a trough bottom and trough walls, the tracks being disposed on left and right sides of the trough bottom.
4. The substrate of claim 3, wherein the groove bottom of the second carrier has a thickness and the groove bottom of the second carrier has a partially or fully transparent bottom.
5. A substrate according to claim 3, characterised in that the height of the track is half the height of the groove wall of the second carrier part.
6. The substrate of claim 1, wherein the width of the second carrier is greater than the width of the first carrier.
7. A system for cryopreservation or thawing resuscitation of biological tissue, comprising: a carrier support and a base;
the carrier support frame is provided with a plurality of groups of fixing grooves, and a plurality of carriers for bearing embryos are fixed in the plurality of groups of fixing grooves;
the substrate includes: the bearing device comprises a first bearing part, a second bearing part and a transition part;
two sides of the bottom of the first bearing part are provided with first positioning plates;
the second bearing part is provided with a track along the length direction of the second bearing part;
the transition part is connected between the first bearing part and the second bearing part in an arc shape; a second positioning plate is arranged at the position, close to the second bearing part, of the transition part;
wherein the first and second positioning plates define the position of the carrier support frame.
8. The system of claim 7, wherein the width of the second carrier is greater than the width of the first carrier.
9. The system of claim 7, further comprising: the carrier, the carrier is including carrying the pole body, carry the pole body including front end thin slice and rear end and carry the pole, the front end thin slice is provided with the recess that bears the embryo.
10. The system of claim 9, wherein the carrier further comprises a sleeve disposed over the carrier rod body.
11. The system of claim 9, wherein the second carrier portion comprises a trough bottom having a partially or fully transparent bottom portion and trough walls, the transparent bottom portion supporting the front sheet of the carrier.
12. The system of claim 7, further comprising: a chip comprising a plurality of compartments, the chip disposed over the track.
13. The system of claim 12, wherein the connection of the first carrier portion and the transition portion defines an initial position of placement of the chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120458131.XU CN215560186U (en) | 2021-03-03 | 2021-03-03 | Substrate and system for cryopreservation or thawing recovery of biological tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120458131.XU CN215560186U (en) | 2021-03-03 | 2021-03-03 | Substrate and system for cryopreservation or thawing recovery of biological tissue |
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| CN215560186U true CN215560186U (en) | 2022-01-18 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023246645A1 (en) * | 2022-06-24 | 2023-12-28 | 深圳拜尔洛克生物技术有限公司 | Biochip moving apparatus |
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2021
- 2021-03-03 CN CN202120458131.XU patent/CN215560186U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023246645A1 (en) * | 2022-06-24 | 2023-12-28 | 深圳拜尔洛克生物技术有限公司 | Biochip moving apparatus |
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