CN215440482U - In-vitro experimental device capable of applying parameterized electric field - Google Patents
In-vitro experimental device capable of applying parameterized electric field Download PDFInfo
- Publication number
- CN215440482U CN215440482U CN202023324588.6U CN202023324588U CN215440482U CN 215440482 U CN215440482 U CN 215440482U CN 202023324588 U CN202023324588 U CN 202023324588U CN 215440482 U CN215440482 U CN 215440482U
- Authority
- CN
- China
- Prior art keywords
- electric field
- culture dish
- applying
- parameterized
- electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides an in vitro experimental device capable of applying a parameterized electric field, which comprises: a culture dish (8) for containing biological tissue cultured in vitro; an even number of electrode plates (14) arranged on the inner wall of the culture dish (8) and used for applying an electric field to the culture solution; and a structural support (15) for fixing the culture dish (8) and the plurality of electrode plates (14). Compared with the prior art, the utility model has the advantages that: setting a culture medium temperature range suitable for cell growth, and intelligently adjusting heat generation quantity according to the temperature change of the culture medium, so that the temperature when heat generation and heat dissipation are balanced falls within the set range, and the method is beneficial to cell culture; according to different set environmental temperatures, electric fields with different parameters can be applied; the culture dish adopts the material that the heat conductivity is high, can accelerate the heat dissipation at the cooling in-process.
Description
Technical Field
The utility model belongs to the field of in-vitro experimental equipment, and particularly relates to an in-vitro experimental research device for applying a parameterized electric field to unicellular organisms, isolated tissues or cells and a temperature control method thereof.
Background
Tumor therapeutic electric fields (TTFields) are a well established therapeutic approach to inhibit cell proliferation by interfering with cell mitosis. The low-intensity, medium-frequency and alternating electric field is used for intervening and treating various tumor diseases, and is a clinically effective cancer treatment method. Many basic studies have been conducted at home and abroad to confirm the biological effects of TTfields. Cell experiment research results show that TTfields can regulate and control biochemical molecule expression in tumor cells, interfere division cycles of the tumor cells, inhibit proliferation of the tumor cells and promote apoptosis of the tumor cells. Animal experiment research results show that TTfields can effectively inhibit the tumor angiogenesis in tumor-bearing mice and inhibit the growth of tumors in the mice. The current results of clinical research at home and abroad also prove the positive effect of TTfields on tumor treatment.
When treating a patient, TTFields couples an electric field into the patient through a transducer array made of multiple ceramic plates with high dielectric constants. However, in vitro studies, the material of the commonly used biological culture device is mainly an insulating material such as plastic or glass, and the TTFields cannot couple the electric field to the single-cell organism, isolated tissue or cells cultured in the device through the insulating material outside the biological culture device. Therefore, how to generate an electric field with uniform distribution inside a commonly used biological culture device becomes a problem to be solved. In addition, the temperature conditions for in vitro culture of biological tissues are relatively strict (usually, the temperature is maintained between 36 and 37 ℃), and the electric field acting on the culture medium can generate a large amount of heat, thereby causing the temperature to rise. Many previous in vitro studies do not take into consideration the effect of heat generation, and biological tissues are cultured in abnormal temperature environment, so that the experimental results lack reference value and comparability. Therefore, the development of an in vitro experimental electrode plate which can uniformly apply a parameterized TTfields electric field (adjustable electric field intensity and frequency) to biological tissues cultured in vitro and keep the temperature of a culture medium constant plays an important role in the research and study of the action mechanism of the TTfields.
SUMMERY OF THE UTILITY MODEL
In order to make up for the defects of the existing in vitro experimental electrode plate, the utility model provides an in vitro experimental device capable of applying a parameterized electric field and a temperature control method thereof. In TTfields in-vitro experiments, the electric field intensity in the culture dish can be automatically adjusted according to the set external temperature, so that the temperature of the culture medium is kept in a temperature range suitable for sample culture, and the parameterized research on TTfields treatment is carried out.
In order to achieve the above object, a first embodiment of the present invention provides an in vitro experiment apparatus capable of applying a parameterized electric field, comprising:
a culture dish (8) for containing biological tissue cultured in vitro;
an even number of electrode plates (14) arranged on the inner wall of the culture dish (8) and used for applying an electric field to the culture solution;
and the structure support (15) is used for fixing the culture dish (8) and the plurality of electrode plates (14).
According to one aspect of the utility model, the electrode plate (14) comprises a circuit board (5), a conductive terminal (9), an electrode (12), a dielectric layer (6) and a heat-sensitive sensor (7), wherein
The conductive terminal (9) is configured to receive an electrical signal from the outside and transmit it to the electrode (12), and transmit the signal sensed by the heat-sensitive sensor (7) to the outside;
the electrode (12) is configured to receive an electrical signal from an electrically conductive terminal (9) and to apply an electrical signal to the dielectric layer (6); and
the heat sensitive sensor (7) is configured to sense an ambient temperature and transmit to the electrically conductive terminal (9).
According to one aspect of the utility model, the structural support (15) comprises an electrode insert plate (1), support columns (3) and a base (4), the support columns (3) support the electrode insert plate (1) on the base (4) to accommodate the culture dish (8), and the electrode plate (14) is electrically connected with the electrode insert plate (1) through the conductive terminals (9).
According to one aspect of the utility model, the even number of electrode plates (14) are arranged in pairs opposite to each other in the culture dish (8).
According to one aspect of the utility model, the conductive terminals 9, electrodes 12, dielectric layer 6 and thermal sensor 7 are on the circuit board 5.
According to one aspect of the utility model, the electrode 12 is a partially exposed conductive portion on the circuit board 5, and the dielectric layer 6 is attached to the surface of the electrode 12.
According to an aspect of the utility model, further conductive layers 13 may be included between the electrodes 12 and the dielectric layer 6.
According to one aspect of the utility model, the dielectric layer 6 has a dielectric constant greater than 1000.
According to one aspect of the utility model, the dielectric layer 6 has a dielectric constant of 24000.
According to one aspect of the utility model, the structural support 15 further comprises a top plate 2, said top plate 2 being provided with through holes through which said electrode plates 14 can pass and be placed in said culture dish 8.
According to one aspect of the utility model, the base 4 has a recess of the same shape and dimensions as the bottom outer wall of the culture dish 8 for holding the culture dish 8.
According to one aspect of the utility model, the culture dish 8 is made of a material having a high thermal conductivity.
According to one aspect of the utility model, the material of the culture dish is quartz glass or ceramic.
A second embodiment of the present invention provides a method for controlling a temperature of an in vitro experimental apparatus capable of applying a parameterized electric field, comprising the following steps:
setting a temperature value controlled by a temperature control unit, wherein the temperature value is expressed by SV, a temperature monitoring unit collects N temperature values in a sampling period T and feeds the N temperature values back to the temperature control unit, the temperature monitoring unit is expressed by PV, the temperature control unit receives temperature information and carries out PV linear fitting algorithm processing with respect to time T, and the following formula is satisfied: PV ═ atn+C，
Wherein a, n and C are constants, PV is SV in temperature dynamic balance, if PV is not SV, the adjustment is carried out in four cases,
(1) when a is 0, PV is C, the internal temperature of the culture dish is constant, if C is SV, the output intensity of an electric signal is increased, and the heat generation quantity is increased until C is SV; if C is greater than SV, reducing the output intensity of the electric signal and reducing the heat generation quantity until C is equal to SV;
(2) when a is greater than 0 and n is 0, PV is a + C, and the process is the same as (1);
(3) when a is>0，n>0 or a<0，n<At 0, PV ═ atn+ C, the PV is rising, when the temperature inside the dish is rising, if PV is<SV, slowly increasing the output intensity of the electric signal and slowly increasing the heat generation quantity, so that the PV is accelerated and slowed down and tends to SV until the PV is SV; if PV>SV, wherein the output intensity of the electric signal is reduced, the heat generation quantity is reduced, and the PV is reduced and tends to SV until the PV is SV;
(4) when a is greater than 0, n is less than 0 or a is less than 0, n is greater than 0, PV is in a descending trend, the temperature in the culture dish is in a descending state, if PV < SV, the output intensity of an electric signal is increased, the heat generation quantity is increased, and the PV is increased and tends to SV until the PV is equal to SV; if PV is greater than SV, the output intensity of the electric signal is slowly reduced, and the heat generation amount is slowly reduced, so that the PV slows down and approaches SV until the PV is equal to SV.
Compared with the prior art, the utility model has the advantages that:
setting a culture medium temperature range suitable for cell growth, and intelligently adjusting heat generation quantity according to the temperature change of the culture medium, so that the temperature when heat generation and heat dissipation are balanced falls within the set range, and the method is beneficial to cell culture;
according to different set environmental temperatures, electric fields with different parameters can be applied;
the culture dish adopts the material that the heat conductivity is high, can accelerate the heat dissipation at the cooling in-process.
Drawings
FIG. 1 is a perspective view showing a temperature-controlled electrode plate of an in vitro experimental apparatus capable of applying a parameterized electric field according to a first embodiment of the present invention;
FIG. 2 is an exploded perspective view showing the electrodes 12 and the dielectric layer 6 of the temperature-controlled electrode plate of an in vitro experimental apparatus capable of applying a parameterized electric field according to the first embodiment of the present invention;
FIG. 3 is a perspective view showing that the electrode 12 of the temperature-controlled electrode plate of the in vitro experiment device for applying a parameterized electric field and the dielectric layer 6 further comprise other conductive layers 13;
FIG. 4 shows a perspective view of an in vitro experimental apparatus capable of applying a parameterized electric field according to a first embodiment of the present invention, wherein an electrode insert plate 1 and a base plate 4 in a structural support 15 are inserted tightly through a support column 3 to accommodate a culture dish 8;
fig. 5 shows a perspective view of an in vitro experimental setup with the possibility of applying a parameterised electric field according to a first embodiment of the utility model, in which the electrode insert plate 1 in the structural support 15 is separated from the base plate 4;
FIG. 6 shows a cross-sectional view of an in vitro assay device capable of applying a parameterized electric field according to a first embodiment of the utility model; and
fig. 7 shows a perspective view of an in vitro experimental apparatus capable of applying a parameterized electric field according to a first embodiment of the present invention, including a top plate.
Reference numerals: 1-electrode plug board, 2-top board, 3-support column, 4-base, 5-circuit board, 6-dielectric layer, 7-thermosensitive sensor, 8-culture dish, 9-conductive terminal, 10-wiring terminal, 11-nylon screw, 12-electrode, 13-other conductive layer, 14-electrode plate and 15-structure support
Detailed Description
In the following description, for purposes of explanation, numerous specific details are set forth in order to provide a thorough understanding of one or more embodiments. It may be evident, however, that such embodiment(s) may be practiced without these specific details. In other instances, well-known structures and devices are shown in block diagram form in order to facilitate describing one or more embodiments.
Various embodiments according to the present invention will be described in detail below with reference to the accompanying drawings.
The first embodiment of the present invention provides an in vitro experimental apparatus capable of applying a parameterized electric field, comprising: a culture dish 8 for accommodating biological tissue cultured in vitro; an electrode plate 14; the structure support 15 comprises an electrode inserting plate 1, a supporting column 3 and a base 4, wherein the supporting column 3 supports the electrode inserting plate 1 on the base 4 to contain the culture dish 8, and the electrode plate 14 is electrically connected with the electrode inserting plate 1 through the conductive terminals 9.
Fig. 4 shows a perspective view of an in vitro experimental setup with the possibility of applying a parameterised electric field according to a first embodiment of the utility model, wherein the electrode insert plate 1 in the structural support 15 is inserted tightly with the base plate 4 through the support columns 3 to accommodate the culture dish 8. Fig. 5 shows a perspective view of an in vitro experimental setup with the possibility of applying a parameterised electric field according to a first embodiment of the utility model, wherein the electrode insert plate 1 in the structural support 15 is separated from the base plate 4. Fig. 6 shows a cross-sectional view of an in vitro experimental device according to a first embodiment of the present invention, to which a parameterised electric field can be applied.
The structure of each component is described in detail below.
Fig. 1 shows a perspective view of an electrode plate 14 in a first embodiment according to the present invention. As shown in fig. 1, the electrode plate 14 includes a circuit board 5, a conductive terminal 9, an electrode 12, a dielectric layer 6, and a heat sensor 7, wherein the conductive terminal 9 is configured to receive an electrical signal from the outside and transmit the electrical signal to the electrode 12, and transmit a signal sensed by the heat sensor 7 to the outside; the electrodes 12 are configured to receive electrical signals from the electrically conductive terminals 9 and to apply electrical signals to the dielectric layer 6; and the heat-sensitive sensor 7 is configured to sense the ambient temperature and transmit to the conductive terminal 9.
According to one embodiment, the conductive terminals 9, electrodes 12, dielectric layer 6 and thermal sensor 7 are on the circuit board 5, as shown in fig. 1.
Fig. 2 shows an exploded perspective view of the electrode 12 and the dielectric layer 6 of the electrode plate 14 in the first embodiment according to the present invention. As shown in fig. 2, the electrode 12 is a partially exposed conductive portion on the circuit board 5, and the dielectric layer 6 is attached to the surface of the electrode 12.
Specifically, the circuit board 5 is a small short PCB, which is in an inverted T shape. The back surface of the electrode is insulated, two round copper layers are left on the bottom of the inverted T-shaped part on the front surface of the electrode to be used as electrodes 12, and the rest parts are insulated. The electrical signals received from the outside are transmitted to the electrodes 12 through the PCB small short board internal wires. As shown in fig. 1.
In particular, the dielectric layer 6 is an electrical signal application medium, which may be a high dielectric constant ceramic sheet. It is not conductive in itself, but due to its high dielectric constant nature, it is suitable for applying alternating electrical signals, creating a capacitive effect. It is in the shape of a cylindrical sheet, as shown in fig. 2. Specifically, the dielectric layers 6 are respectively fixed to the electrodes 12 of the circuit board 5 along the edges thereof using UV glue, and the electrodes 12 transfer charges to the dielectric layers 6 and gather them, thereby forming a capacitive effect between two opposite electrodes 12, and further generating an electric field.
According to one embodiment, the dielectric constant of the dielectric layer 6 is greater than 1000.
According to one embodiment, the dielectric layer 6 has a dielectric constant of 24000.
Fig. 3 is a perspective view showing that another conductive layer 13 is further included between the electrode 12 of the electrode plate 14 and the dielectric layer 6 in the first embodiment according to the present invention. As shown in fig. 3, other conductive layers 13 may also be included between the electrodes 12 and the dielectric layer 6. In particular, the electrode 12 may be covered with a silver layer 13 to increase conductivity.
According to one embodiment, the thermal sensor 7 may be a thermistor, which is located at the bottom of each sheet of electrodes 12, as shown in FIG. 1. The heat sensor 7 senses the internal temperature and transmits the internal temperature to the conductive terminal 9 and further to the external control unit, and the control unit dynamically adjusts the output intensity of the electric signal according to the real-time temperature, so that the heat generated by an electric field is controlled, the internal temperature is adjusted, and the dynamic balance of the required temperature is achieved.
Turning now to fig. 4-7. In each of figures 4 to 7 a culture dish 8 is shown, which is accommodated between the electrode insert plate 1 and the base plate 4 in the structural support 15 for accommodating biological tissue cultured in vitro. As a specific example, the quartz glass culture dish 8 is customized according to specific requirements, is in a square shape, is opened at the top surface and is used for culturing samples, and a bidirectional electric field can be applied. As an example, the length-width-height of the outer wall is 60mm, the length-width-height of the inner wall is 56mm multiplied by 58mm, and the wall thickness is 2 mm. Before use, the mixture is cleaned by an ultrasonic cleaner and then sterilized by a high-temperature high-pressure sterilization pot for standby.
Fig. 4 to 7 also show a structural support 15, which comprises an electrode insert plate 1, a support column 3 and a base 4, wherein the support column 3 supports the electrode insert plate 1 on the base 4 to accommodate the culture dish 8, and the electrode plate 14 is electrically connected with the electrode insert plate 1 through the conductive terminal 9. In fig. 4, the electrode insert plate 1 in the structural support 15 is inserted tightly with the base plate 4 through the support posts 3 to accommodate the culture dish 8. In fig. 5 the electrode insert plate 1 in the structural support 15 is separated from the base plate 4. The structural support 15 shown constitutes the framework for holding the culture dish and the electrodes.
Fig. 7 shows a perspective view of an in vitro experimental apparatus capable of applying a parameterized electric field according to a first embodiment of the utility model, wherein the structural support 15 further comprises a top plate 2. In particular, the structural support 15 further comprises a top plate 2, said top plate 2 being provided with through holes through which said electrode plates 14 can pass and be placed in said culture dish 8. Specifically, the top plate 2, the supporting columns 3 and the base 4 may be made of acrylic material, for example, and have a thickness of 10 mm.
According to one embodiment, the culture dish 8 is made of a material with high thermal conductivity. By way of example, the material of the culture dish may be quartz glass or ceramic, and quartz glass is taken as an example in the present embodiment.
Specifically, the electrode inserting plate 1 is a PCB circuit board for transmitting electric signals to the electrodes 5, and the position of the conductive terminal 9 is determined according to the position of the inner edge of each quartz glass culture dish 8 to connect the electrodes 5. Is connected to an electrical signal generating device through the connection terminal 10 to transmit an electrical signal to each electrode. Wherein, the top plate 2 is perforated according to the shape and the size of the outer wall at the top of the quartz glass culture dish 8, and the length-width of the perforated hole is 62mm multiplied by 62mm in order to increase the air permeability. And the electrode inserting plate 1 is fixed on the top plate by using a nylon screw 11, thereby forming a lid of the quartz glass culture dish 8. Wherein, the support column 3 is fixed on two sides of the edge of the base 4 by nylon screws 11, the height of the support column is consistent with that of the quartz glass culture dish 8 and is 60 mm. The top of the electrode plug board is protruded by 10mm and is clamped into a notch at the edge of a cover consisting of the electrode plug board 1 and the top board 2 for supporting and fixing the cover.
According to one embodiment, the base 4 has a recess of the same shape and dimensions as the outer wall of the bottom of the culture dish 8 for fixing the culture dish 8. Wherein, base 4 sinks according to 8 bottom outer wall shapes of quartz glass culture dish and size and polishes 2mm for confirm the position of quartz glass culture dish 8 and fixed quartz glass culture dish 8 do not slide, and make and leave 2mm gap between 8 tops of quartz glass culture dish and the lid, increase air permeability. The electrodes 5 are connected with the electrode plugboard 1 through the pluggable male and female conductive terminals 9, and the electrodes are convenient to replace.
Wherein, the electrodes 5 are arranged in the quartz glass culture dish 8 with the front opposite at intervals and adjacent to the inner wall of the culture dish to form a uniform electric field.
During the experiment, the sample is cultured in a quartz glass culture dish 8, the quartz glass culture dish 8 is arranged in a sunken groove of a base 4, an electrode 5 is inserted into an electrode inserting plate 1, the electrode inserting plate and a top plate are fixed together by using a nylon screw, and the electrode is aligned with the inside of the culture dish and inserted and simultaneously covered with a culture dish cover consisting of the electrode inserting plate and the top plate. The experimental device can be used for carrying out electric field stimulation experiments on three parallel samples at a time, and can be expanded into electric field stimulation experiments on more than three parallel samples according to requirements. The electric field stimulation of the sample can be performed by connecting the terminal to the electric signal generating device, setting parameters and starting the electric signal generating device, as shown in fig. 7.
A second embodiment of the present invention provides a method for controlling a temperature of an in vitro experimental apparatus capable of applying a parameterized electric field, comprising the following steps:
the method comprises the following steps of setting a temperature value controlled by a temperature control unit, wherein the temperature value is expressed by SV, the temperature monitoring unit collects N temperature values in a sampling period T and feeds the N temperature values back to the temperature control unit, the collected temperature value is expressed by PV, the temperature control unit receives temperature information and carries out PV linear fitting algorithm processing with respect to time T, and the following formula is satisfied: PV ═ atn+C，
Wherein a, n and C are constants, PV is SV in temperature dynamic balance, if PV is not SV, the adjustment is carried out in four cases,
(1) when a is 0, PV is C, the internal temperature of the culture dish is constant, if C is SV, the output intensity of an electric signal is increased, and the heat generation quantity is increased until C is SV; if C is greater than SV, reducing the output intensity of the electric signal and reducing the heat generation quantity until C is equal to SV;
(2) when a is greater than 0 and n is 0, PV is a + C, and the process is the same as (1);
(3) when a is>0，n>0 or a<0，n<At 0, PV ═ atn+ C, the PV is rising, when the temperature inside the dish is rising, if PV is<SV, slowly increasing the output intensity of the electric signal and slowly increasing the heat generation quantity, so that the PV is accelerated and slowed down and tends to SV until the PV is SV; if PV>SV, wherein the output intensity of the electric signal is reduced, the heat generation quantity is reduced, and the PV is reduced and tends to SV until the PV is SV;
(4) when a is greater than 0, n is less than 0 or a is less than 0, n is greater than 0, PV is in a descending trend, the temperature in the culture dish is in a descending state, if PV < SV, the output intensity of an electric signal is increased, the heat generation quantity is increased, and the PV is increased and tends to SV until the PV is equal to SV; if PV is greater than SV, the output intensity of the electric signal is slowly reduced, and the heat generation amount is slowly reduced, so that the PV slows down and approaches SV until the PV is equal to SV.
The utility model provides an in-vitro experimental device capable of applying a parameterized electric field and a temperature control method thereof, wherein the TTfields electric field is directly applied to unicellular organisms, isolated tissues or cells so as to influence the growth of the unicellular organisms, the isolated tissues or the cells. In TTfields in-vitro experiments, the electric field intensity in the culture dish can be automatically adjusted according to the set external temperature, so that the temperature of the culture medium is kept in a temperature range suitable for sample culture, and the parameterized research on TTfields treatment is carried out. The electrode plate has the advantages of large adjustable range of electric field intensity and frequency, simple operation, low manufacturing cost, easy realization and accurate temperature control, and can provide technical support for basic research and clinical research in the TTfields treatment field.
While the foregoing disclosure shows illustrative embodiments of the utility model, it should be noted that various changes and modifications could be made herein without departing from the scope of the utility model as defined by the appended claims. Furthermore, although elements of the utility model may be described or claimed in the singular, the plural is contemplated unless limitation to a single element is explicitly stated.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the utility model. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (13)
1. An in vitro assay device capable of applying a parameterized electric field, comprising:
a culture dish (8) for containing biological tissue cultured in vitro;
an even number of electrode plates (14) arranged on the inner wall of the culture dish (8) and used for applying an electric field to the culture solution; and
and the structure support (15) is used for fixing the culture dish (8) and the plurality of electrode plates (14).
2. In vitro experimental device capable of applying parameterized electric fields according to claim 1, characterized in that the electrode plates (14) comprise a circuit board (5), conductive terminals (9), electrodes (12), dielectric layers (6) and a thermo sensor (7), wherein
The conductive terminal (9) is configured to receive an electrical signal from the outside and transmit it to the electrode (12), and transmit the signal sensed by the heat-sensitive sensor (7) to the outside;
the electrode (12) is configured to receive an electrical signal from an electrically conductive terminal (9) and to apply an electrical signal to the dielectric layer (6); and
the heat sensitive sensor (7) is configured to sense an ambient temperature and transmit to the electrically conductive terminal (9).
3. The in vitro experimental device capable of applying a parameterized electric field according to claim 2, wherein the structural support (15) comprises an electrode insert plate (1), support columns (3) and a base (4), the support columns (3) support the electrode insert plate (1) on the base (4) to accommodate the culture dish (8), and the electrode plate (14) is electrically connected with the electrode insert plate (1) through the conductive terminals (9).
4. The in vitro experimental device capable of applying a parameterized electric field according to claim 1, wherein an even number of the electrode plates (14) are arranged in pairs in a culture dish (8).
5. The in-vitro experimental device capable of applying parameterized electric fields according to claim 3, characterized in that the conductive terminals (9), the electrodes (12), the dielectric layer (6) and the thermosensors (7) are on the circuit board (5).
6. The in-vitro experimental device capable of applying parameterized electric fields according to claim 3, wherein the electrodes (12) are partially exposed conductive parts on the circuit board (5), and the dielectric layer (6) is attached to the surface of the electrodes (12).
7. The in-vitro experimental device capable of applying a parameterized electric field according to claim 2, characterized in that further conductive layers (13) may be included between the electrodes (12) and the dielectric layer (6).
8. In vitro experimental device according to claim 2, wherein the dielectric layer (6) has a dielectric constant greater than 1000.
9. The in vitro experimental device capable of applying parameterized electric field according to claim 8, characterized in that the dielectric constant of the dielectric layer (6) is 24000.
10. The in vitro experimental apparatus capable of applying a parameterized electric field according to claim 1, wherein the structural support (15) further comprises a top plate (2), the top plate (2) is provided with a through hole, and the electrode plate (14) can pass through the through hole and be placed in the culture dish (8).
11. The in vitro experimental apparatus capable of applying parameterized electric field according to claim 3, characterized in that the base (4) has grooves with the same shape and size as the outer wall of the bottom of the culture dish (8) to fix the culture dish (8).
12. The in vitro experimental apparatus capable of applying parameterized electric field according to claim 1, characterized in that the culture dish (8) is made of a material with high thermal conductivity.
13. The in vitro experimental apparatus capable of applying parameterized electric field according to claim 12, wherein the material of the culture dish is quartz glass or ceramic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023324588.6U CN215440482U (en) | 2020-12-31 | 2020-12-31 | In-vitro experimental device capable of applying parameterized electric field |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023324588.6U CN215440482U (en) | 2020-12-31 | 2020-12-31 | In-vitro experimental device capable of applying parameterized electric field |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215440482U true CN215440482U (en) | 2022-01-07 |
Family
ID=79681663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202023324588.6U Active CN215440482U (en) | 2020-12-31 | 2020-12-31 | In-vitro experimental device capable of applying parameterized electric field |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215440482U (en) |
-
2020
- 2020-12-31 CN CN202023324588.6U patent/CN215440482U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112680349A (en) | In-vitro experiment device capable of applying parameterized electric field and temperature control method thereof | |
| US20180202991A1 (en) | System for Viewing Cell Cultures under a Microscope Whilst Applying TTFields | |
| CN210458213U (en) | Biological culture system of electricity environment | |
| CN102389618B (en) | Electrodes for applying an electric field in-vivo over an extended period of time | |
| Andocs et al. | Nanoheating without artificial nanoparticles part II. Experimental support of the nanoheating concept of the modulated electro-hyperthermia method, using U937 cell suspension model | |
| CN107734722B (en) | Graphene heating sheet and preparation method thereof | |
| CN215440482U (en) | In-vitro experimental device capable of applying parameterized electric field | |
| CN110016436A (en) | A kind of electrical environment biological culture system | |
| CN203768368U (en) | High-throughput electric stimulation instrument for cell culture | |
| US4889691A (en) | Modular tissue superfusion chamber | |
| CN103747388A (en) | Sterilizing and drying device for hearing aid | |
| CN214400545U (en) | Temperature control electrode plate capable of applying parametric electric field and system | |
| CN212610626U (en) | Energy-conserving biological bacterial constant temperature equipment of high efficiency | |
| CN102914696A (en) | Biological tissue impedance temperature characteristic test system based on water circulation thermostat | |
| CN115505505A (en) | Electric environment culture dish and culture device with same | |
| CN108795753A (en) | A kind of device and method of encapsulation plane electrode chip | |
| CN106367344B (en) | A kind of cell minute yardstick heating culture control device | |
| CN209144174U (en) | A kind of cell Heating Experiment cell culture apparatus | |
| CN209423673U (en) | A kind of small-size multifunction experiment porch | |
| CN219314958U (en) | Reusable tumor electric field treatment in-vitro cell experimental device | |
| CN219010329U (en) | Biological tissue treatment device | |
| CN115109700A (en) | Special cell culture dish of tumour treatment electric field basic experiment | |
| CN209270647U (en) | A kind of tumor suppression device of process control | |
| CN213641386U (en) | Multifunctional animal operating table | |
| CN109433294A (en) | A kind of small-size multifunction experiment porch |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |