CN209270647U - A kind of tumor suppression device of process control - Google Patents
A kind of tumor suppression device of process control Download PDFInfo
- Publication number
- CN209270647U CN209270647U CN201820793084.2U CN201820793084U CN209270647U CN 209270647 U CN209270647 U CN 209270647U CN 201820793084 U CN201820793084 U CN 201820793084U CN 209270647 U CN209270647 U CN 209270647U
- Authority
- CN
- China
- Prior art keywords
- magnetic field
- field strength
- frequency
- monitoring
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004886 process control Methods 0.000 title claims abstract description 15
- 230000005760 tumorsuppression Effects 0.000 title claims abstract description 11
- 238000012544 monitoring process Methods 0.000 claims abstract description 46
- 238000012545 processing Methods 0.000 claims abstract description 21
- 230000010355 oscillation Effects 0.000 claims abstract description 8
- 238000009423 ventilation Methods 0.000 claims abstract description 7
- 230000009514 concussion Effects 0.000 claims description 11
- 230000003068 static effect Effects 0.000 abstract description 22
- 230000002401 inhibitory effect Effects 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 9
- 210000004881 tumor cell Anatomy 0.000 abstract description 7
- 230000009183 running Effects 0.000 abstract description 3
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 23
- 230000005855 radiation Effects 0.000 description 21
- 206010028980 Neoplasm Diseases 0.000 description 9
- 230000005672 electromagnetic field Effects 0.000 description 9
- 230000008859 change Effects 0.000 description 7
- 208000008383 Wilms tumor Diseases 0.000 description 6
- 208000022013 kidney Wilms tumor Diseases 0.000 description 6
- 201000008026 nephroblastoma Diseases 0.000 description 6
- 230000010261 cell growth Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 102400000888 Cholecystokinin-8 Human genes 0.000 description 3
- 101800005151 Cholecystokinin-8 Proteins 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 230000004087 circulation Effects 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- IZTQOLKUZKXIRV-YRVFCXMDSA-N sincalide Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](N)CC(O)=O)C1=CC=C(OS(O)(=O)=O)C=C1 IZTQOLKUZKXIRV-YRVFCXMDSA-N 0.000 description 3
- 230000001629 suppression Effects 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 238000013043 cell viability test Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000009098 adjuvant therapy Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000007321 biological mechanism Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011281 clinical therapy Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 230000004770 neurodegeneration Effects 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 210000004498 neuroglial cell Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000000015 thermotherapy Methods 0.000 description 1
- 230000001875 tumorinhibitory effect Effects 0.000 description 1
Landscapes
- Magnetic Treatment Devices (AREA)
Abstract
The utility model provides a kind of tumor suppression device of process control, by AC power source, booster converter, frequency oscillation generator, DC/AC all-wave inverter, half-wave inverter, output element, coil, temperature-monitoring element and magnetic field strength monitoring element, central processing unit, temperature feedback element and magnetic field strength feedback element, frequency, magnetic field strength and time set element, display, keyboard, ventilation interface and host computer composition.The utility model process control automatic running, irradiated area between two groups of coils generates the magnetic field that magnetostatic field is superimposed with alternating magnetic field, alternating magnetic field frequency range is 0.1Hz-200KHz, the strength range of alternating magnetic field is 0-10mT, static magnetic field strength range is 0-50mT, continuous irradiation in 24 hours can be achieved, and temperature, the dynamic of magnetic field strength in irradiated area are monitored.The utility model is designed reasonably, noninvasive toxic side effect is low, can apply in inhibiting tumor cell activity.
Description
Technical field
The utility model belongs to electronic engineering, medicine, biology and physics crossing domain, is related to medical instrument, especially
It is a kind of tumor suppression device of process control.
Background technique
Biological effect and mechanism of the magnetic field with complexity, related with frequency, intensity, action time length etc. (referring to
Anatoly Buchachenko, 2016, Bioelectromagnetics).Different frequency, intensity magnetic field be widely used in
In field of biomedicine disease treatment, adjuvant treatment and health care.Radio frequency, the power frequency electromagnetic field of industry have certain environment and health
Harm, may be related to tumour, neurodegenerative disease generation.High energy high frequency magnetic field based on fuel factor, mainly in microwave
Frequency range can be used for thermotherapy.The pulsed magnetic field of special frequency band has significant shadow to cell film conductivity, gene expression regulation etc.
It rings, especially bone remoulding, bone wound reparation.High-frequency impulse magnetic field can then cause organism obviously to damage.
Specific magnetic fields can be used for clinical therapy of tumor.Therapeutic equipment based on 100-300KHz middle frequency electromagnetic field
(Novocure company, Israel) has passed through European Union and U.S. FDA approval, female thin with initial neuroglia applied to recurrence
Born of the same parents' tumor.Based on 0.1Hz to the Electromagnetic Field Irradiation of 200KHz frequency range, referred to as Low Energy Emission Therapy
(LEET), there is the clinical application history of many years in Europe, discovered in recent years, which has the kinds of tumors such as liver cancer, breast cancer, to be treated
Effect, and to human body toxic side effect low (referring to Alexandre Barbault etc., 2009, J Exp Clin Cancer Res).
The static magnetic field that direct current generates is superimposed with the alternating magnetic field that industrial-frequency alternating current generates, to the internal external of kinds of tumor cells
It is long also to significantly inhibit effect (referring to Lin-Qing Yuan etc., 2018, Bioelectromagnetics).
Novocure company, Israel is used for the electromagnetic field of pleomorphism gliablastoma clinical treatment, and technology is special
Sign is the alternating magnetic field using 100-300KHz (referring to United States Patent (USP) US8019414, US8406870).In addition, one can produce
The device of 1-1000Hz and 1-100mT electromagnetic field can also intervene cell survival (referring to United States Patent (USP) US8192969).It is Chinese special
Also there are many field generator for magnetic and purposes that design has tumor inhibitory effect in benefit, including one be greater than 0.4 it is special this
The high-intensity magnetic field (referring to Chinese patent 90100152.X) for drawing (T) and a radio-frequency pulse magnetic field are (referring to Chinese patent
94111668.9) and the moderate strength magnetic field that is superimposed with D.C. magnetic field of a power frequency electromagnetic field is (referring to Chinese patent
201720112204.3) etc..
Summary of the invention
The purpose of the utility model is to provide a kind of tumor suppression devices of process control, are a kind of having for process control
The field generator for magnetic of tumor inhibition effect, frequency range cover ultralow frequency tremendously low frequency, can produce as low as middle intensity magnetic field
It acts on organism and induces biological effect, which can produce magnetic field inhibited to tumour in vivo and in vitro.
The utility model provides a kind of tumor suppression device of process control, and composition includes an AC power source 1, first
Booster converter (exchange AC/ direct current DC) the 2, second booster converter (exchange AC/ direct current DC) 3, first frequency shake generator
4, second frequency shakes generator 5, DC/AC all-wave inverter 6, half-wave inverter 7, the first output element 8, the second output element
9, first group of coil, 10, second groups of coils 11, temperature-monitoring element 12, the first magnetic field strength monitoring element (monitoring alternating magnetic field)
13, the second magnetic field strength monitoring element (monitoring static magnetic field) 14, temperature feedback element 15, the first magnetic field strength feedback element
(feedback alternating magnetic field) 16, second magnetic field strength feedback element (feedback static magnetic field) 17, central processing unit 18, frequency setting member
Part 19, magnetic field strength setting element 20, time setting element 21, display 22, keyboard 23, ventilation interface 24 and host computer
25；AC power source 1 is connect with one end of the first booster converter (AC/DC) 2 and the second booster converter (AC/DC) 3 respectively, the
The other end of one booster converter (AC/DC) 2 and the second booster converter (AC/DC) 3 shakes generator 4 with first frequency respectively
It is connected with second frequency concussion generator 5, first frequency shakes the other end point of generator 4 and second frequency concussion generator 5
It is not connect with DC/AC all-wave inverter 6 and half-wave inverter 7, the other end point of DC/AC all-wave inverter 6 and half-wave inverter 7
It is not connect with the first output element 8 and the second output element 9, the other end difference of the first output element 8 and the second output element 9
It is connect with first group of coil 10 and second group of coil 11, is placed with temperature monitoring member in interior irradiated area between two groups of coils
Part 12, the first magnetic field strength monitoring element 13 and the second magnetic field strength monitoring element 14, temperature-monitoring element 12, the first magnetic field are strong
It spends monitoring element (monitoring alternating magnetic field) 13 and the second magnetic field strength monitoring element (monitoring static magnetic field) 14 is anti-with temperature respectively
Present element 15, the first magnetic field strength feedback element 16 and the connection of the second magnetic field strength feedback element 17, temperature feedback element 15, the
The other end of one magnetic field strength feedback element 16 and the second magnetic field strength feedback element 17 is connect with central processing unit 18, center
The other rate of connections of processor 18 sets element 19, magnetic field strength setting element 20 and time setting element 21, frequency setting member
The other end of part 19 is separately connected first frequency concussion generator 4 and second frequency concussion generator 5, and magnetic field strength sets element
20 and the time setting element 21 the other end be separately connected DC/AC all-wave inverter 6 and half-wave inverter 7, central processing unit 18
In addition display 22 and keyboard 23 are connected, and is connected by ventilation interface 24 with host computer 25.
AC power source becomes direct current by the first boosting inverter element, by being exported after frequency oscillation generator frequency conversion,
Then direct current is passed through as alternating current by the switch of 4 process controls by DC/AC all-wave inverter and the first output element
It is output to first coil, generates the alternating magnetic field of direction change.
AC power source becomes direct current by the second boosting inverter element, by being exported after frequency oscillation generator frequency conversion,
Then direct current is output to by the second coil by half-wave inverter and the second output element, generates the constant static-magnetic in direction
?.
By transformer, frequency oscillation generator, DC/AC all-wave inverter, half-wave inverter and coil, in two groups of coils
Between irradiated area inside generate the static state (come from direct current) of superposition and (come from and exchange) electromagnetic field with alternation, wherein alternating magnetic field
Strength Changes range be 0-10mT, static magnetic field strength variation range is 0-50mT.
Instruction can be assigned to central processing unit by keyboard, by frequency setting element, magnetic field strength set element and
Time sets element, carries out sequencing setting to the magnetic field generated inside the irradiated area between two groups of coils, wherein alternation magnetic
The frequency range of field is 0.1Hz to 200kHz, and voltage change range is 0 to 450V, and time change range is 30 seconds to 999
Minute, program runs recurring number 1 to 200, it can be achieved that the continuous magnetic field radiation of daily sequencing in 24 hours.
Central processing unit and host computer can be integrated, program setting and storage be carried out by computer, to replace
For the function of keyboard and display.
In program setting link, Any Digit can be inputted by keyboard, editor's adjusting be carried out to Digital size, and confirm
Input, display or computer screen can show program number, voltage, frequency, time, the recurring number of setting.
The program set, which can number, is stored into central processing unit, and directly recalls operation at any time.
There is temperature-monitoring element, alternating magnetic field intensity monitoring element and static state inside irradiated area between two groups of coils
Magnetic field strength monitoring element, and pass through temperature feedback element, alternating magnetic field intensity feedback element and static magnetic field strength feedback member
Data are returned to central processing unit respectively by part in real time, are realized and are monitored to the dynamic of temperature in irradiated area and magnetic field strength, simultaneously
The information of collection is stored into central processing unit, transfers for later period tracking.
When equipment runs link, and display or computer screen can be numbered with Dynamically Announce current operation program, be run
Real time temperature (DEG C), alternating magnetic field intensity (mT) and static magnetic field strength (mT) in long, irradiated area.
A kind of tumor suppression device of process control provided by the utility model can be applied in inhibiting tumor cell activity,
The tumour cell is people's nephroblastoma cell.Device generates the magnetic field or single that alternating magnetic field is superimposed with static magnetic field
Alternating magnetic field or static magnetic field after implementing continuous magnetic field radiation, generate significant inhibiting effect to growth of human tumor cells, inhibit
Effect power is positively correlated with magnetic field radiation length of time.
The utility model covers the effective electromagnetic field frequency section with tumor inhibition effect, incorporate alternating magnetic field with
Static magnetic field, setting real time monitoring and feedback in magnetic field exposed area, can be to time, frequency, magnetic by central control system
Field intensity is preset, and is realized and is continued magnetic field radiation, periodical frequency modulation and change magnetic field strength, has after use to human tumor cells
There is obvious inhibiting effect.The utility model specifically has the beneficial effect that: (1) utility model device design is rationally, easy to operate,
By changing voltage, frequency, time, recurring number, multiple and different loading schemes can be stored, recall operation at any time；(2) instrument according to
Setting program can daily 24 hours automatic runnings, it can be achieved that the continuous irradiation of program magnetizing field；(3) to magnetic field in irradiated area
Temperature and magnetic field strength do continuous monitoring and Real-time Feedback, have traceable record, securely and reliably；(4) it is placed in irradiated area
Organism is disturbed small, hurtless measure；(5) there is significant inhibiting effect to human tumour.
Detailed description of the invention
Fig. 1 is the tumor suppression apparatus structure schematic diagram of the utility model process control.
Fig. 2 is utility model device the first output element structural schematic diagram.
Fig. 3 is utility model device the second output element structural schematic diagram.
Fig. 4 is utility model device display schematic diagram, different in program setting and the display of instrument automatic running link
Parameter.
Fig. 5 is utility model device keyboard schematic diagram.
Fig. 6 is the conceptual scheme using the irradiation of utility model device implementation magnetic field.
Fig. 7 is that the present apparatus is applied to tri- days cell growth curves of people's nephroblastoma cell G401 and inhibiting rate curve graph.
Specific embodiment
The utility model is further described with reference to the accompanying drawings and examples.
Embodiment 1, the utility model provide a kind of tumor suppression device of process control
Referring to Fig. 1, device composition includes an AC power source 1, the first booster converter (exchange AC/ direct current DC) 2, second
Booster converter (exchange AC/ direct current DC) 3, first frequency concussion generator 4, second frequency shake generator 5, DC/AC all-wave
Inverter 6, half-wave inverter 7, the first output element 8,9, first groups of the second output element, 10, second groups of coil coil 11, temperature
Spend monitoring element 12, the first magnetic field strength monitoring element (monitoring alternating magnetic field) 13, second magnetic field strength monitoring element (monitors quiet
State magnetic field) 14, temperature feedback element 15, the first magnetic field strength feedback element (feedback alternating magnetic field) 16, second magnetic field strength it is anti-
Feedback element (feedback static magnetic field) 17, central processing unit 18, frequency setting element 19, magnetic field strength setting element 20, time set
Determine element 21, display 22, keyboard 23, ventilation interface 24 and host computer 25, AC power source 1 and the first booster converter
(AC/DC) it 2 is connected with one end of the second booster converter (AC/DC) 3, the first booster converter (AC/DC) 2 and the second boosting become
The other end of parallel operation (AC/DC) 3 shakes generator 4 with first frequency respectively and second frequency concussion generator 5 is connect, the first frequency
Rate shake generator 4 and second frequency concussion generator 5 the other end respectively with DC/AC all-wave inverter 6 and half-wave inverter 7
Connection, the other end of DC/AC all-wave inverter 6 and half-wave inverter 7 respectively with the first output element 8 and the second output element 9
The other end of connection, the first output element 8 and the second output element 9 connects with first group of coil 10 and second group of coil 11 respectively
Connect, between two groups of coils in irradiated area in be placed with temperature-monitoring element 12, the first magnetic field strength monitoring element 13 and the
Two magnetic field strength monitoring elements 14, temperature-monitoring element 12, the first magnetic field strength monitoring element (monitoring alternating magnetic field) 13 and the
Two magnetic field strength monitoring elements (monitoring static magnetic field) 14 respectively with temperature feedback element 15, the first magnetic field strength feedback element 16
It is connected with the second magnetic field strength feedback element 17, temperature feedback element 15, the first magnetic field strength feedback element 16 and the second magnetic field
The other end of intensity feedback element 17 is connect with central processing unit 18, and the other rate of connections of central processing unit 18 sets element
19, magnetic field strength setting element 20 and time set element 21, and the other end of frequency setting element 19 is separately connected first frequency
It shakes generator 4 and second frequency shakes generator 5, magnetic field strength sets the other end point of element 20 and time setting element 21
Not Lian Jie DC/AC all-wave inverter 6 and half-wave inverter 7, the other connection display 22 and keyboard 23 of central processing unit 18, or
It is connected using ventilation interface 24 with host computer 25, to replace display 22 and keyboard 23.
AC power source becomes direct current by the first boosting inverter element, by being exported after frequency oscillation generator frequency conversion,
Then as shown in Fig. 2, direct current to be passed through to the switch of 4 process controls by DC/AC all-wave inverter and the first output element
As alternating current, the combination of switch 1,3 is combined with switch 2,4 to be opened and closed automatically in turn, when only switch 1,3 is opened, first group
Current direction is that from top to bottom, and when only switch 2,4 is opened, current direction is from bottom to top, such as in first group of coil in coil
It is alternating current in this first group of coil, generates the alternating magnetic field of direction change.
AC power source becomes direct current by the second boosting inverter element, by being exported after frequency oscillation generator frequency conversion,
Then as shown in figure 3, direct current is output to the by the second output element of half-wave inverter and only one switch control
Two groups of coils generate the constant static magnetic field in direction.
By transformer, frequency oscillation generator, DC/AC all-wave inverter, half-wave inverter and coil, in two groups of coils
Between irradiated area inside generate the static state (come from direct current) of superposition and (come from and exchange) electromagnetic field with alternation, wherein alternating magnetic field
Strength Changes range be 0-10mT, static magnetic field strength variation range is 0-50mT.
By keyboard shown in display as shown in Figure 4 and Fig. 5, or by host computer, frequency is carried out through central processing unit
Rate, magnetic field strength, irradiation time setting, wherein the frequency range of alternating magnetic field is 0.1Hz to 200kHz, voltage change model
Enclosing is 0 to 450V, and time change range is 30 seconds to 999 minutes, and program runs recurring number 1 to 200, it can be achieved that daily 24 hours
The continuous magnetic field radiation of sequencing can input Any Digit by keyboard or host computer in editing procedure, big to number
Small to carry out editor's adjusting, and confirmation input, the program set, which can number, is stored into central processing unit, and directly recalls at any time
Operation.
Continuous monitoring and note are done when equipment runs specific program, while to temperature in irradiated area and magnetic field strength
Record: sensor includes that temperature-monitoring element, the alternating magnetic field intensity monitoring in the irradiated area being placed between two groups of coils are first
Part and static magnetic field strength monitoring element, and pass through temperature feedback element, alternating magnetic field intensity feedback element and static-magnetic field strength
Feedback element is spent, data are returned into central processing unit in real time respectively, display or computer screen can currently be transported with Dynamically Announce
Line program number, operation duration, real time temperature (DEG C), alternating magnetic field intensity (mT) and static magnetic field strength in irradiated area
(mT), the real time monitoring to temperature in irradiated area and magnetic field strength is realized, while information is stored into central processing unit, for day
After track.
Embodiment 2, the utility model act on the people nephroblastoma cell G401 of culture, fill using shown in Fig. 1-Fig. 5
It sets, the static magnetic field of varying strength is superimposed integration with alternating magnetic field, daily irradiation 2-8 hours；Control group is by identical cultivation conditions
Cell be placed in the irradiated area for being not powered on the same sampling device for not generating magnetic field, the time is identical.
Specific implementation process is:
Cell line and cultural method: people nephroblastoma cell G401 is cultivated using the McCoy containing 10% fetal calf serum
Base is incubated in 37 DEG C of incubators containing 5% carbon dioxide and saturated steam.
Capital equipment: the tumor suppression device of process control, cell incubator, viable count instrument, multi-function microplate reader.
Main agents: CCK8 kit, trypan blue solution.
Magnetic field loading method: T1 5mT, T2 4.5mT, T3 4mT, T4 3.5mT, T5 3mT, T6 4.5mT, T7 4mT,
T8 3.5mT, T9 3mT, T10 5mT, T11 4mT, T12 3.5mT, T13 3mT, T14 5mT, T15 4.5mT, T16
3.5mT, T17 3mT, T18 5mT, T19 4.5mT, T20 4mT, T21 3mT, T22 5mT, T23 4.5mT, T24 4mT, T25
3.5mT。
Alternating magnetic field loading method: frequency 50Hz, T1 1mT, T2 1.5mT, T3 2mT, T4 2.5mT, T5 3mT, T6
1mT, T7 1.5mT, T8 2mT, T9 2.5mT, T10 3mT, T11 1mT, T12 1.5mT, T13 2mT, T14 2.5mT, T15
3mT, T16 1mT, T17 1.5mT, T18 2mT, T19 2.5mT, T20 3mT, T21 1mT, T22 1.5mT, T23 2mT, T24
2.5mT, T25 3mT.
It is exposed in the magnetic field environment of static magnetic field superposition integration synchronous with alternating magnetic field by according to object, concrete scheme such as Fig. 6
It is shown.
T1-T25: 5 minutes each periods；Daily 1 circulation of 2 hour/day magnetic field radiation experimental groups；4 hour/day magnetic field spokes
According to daily 2 circulations of experimental group；Daily 4 circulations of 8 hour/day magnetic field radiation experimental groups.
Cell viability tests (living cells count): by people nephroblastoma cell G401 with the close of 1 × 10 5/hole ∧
Degree is inoculated in 6 orifice plates, and experimental group cell gives 2 hours/day, 4 hours/day and 8 hours/day according to scheme shown in Fig. 6 respectively
Magnetic field radiation, cellular control unit are placed in the irradiated area for not generating magnetic field, continuously apply magnetic field radiation 3 days, are irradiated daily
It collects cell at once afterwards, after Trypan Blue, with viable count instrument meter number, makes cell Proliferation curve and magnetic field radiation to thin
The suppression curve of born of the same parents.
Cell viability tests (CCK8 method): people nephroblastoma cell G401 is inoculated with the density in 1 × 10 5/hole ∧
In 6 orifice plates, experimental group cell gives 2 hours/day, 4 hours/day and 8 hour/day magnetic field spokes according to scheme shown in Fig. 6 respectively
According to cellular control unit is placed in the irradiated area for not generating magnetic field, continuously applies magnetic field radiation 3 days, daily after irradiation, is used
CCK8 kits absorbance calculates cell relative number according to reading, makes cell Proliferation curve and magnetic field to cell
Suppression curve.
Statistical analysis: every kind of magnetic field radiation condition is repeated 3 times, every time 3 multiple holes, as a result with average value ± standard error table
Show, as a result do T inspection, using P < 0.05 as with significant difference index.
As shown in fig. 7, left hand view is G401 cell growth curve, wherein X-axis is magnetic field radiation number of days, and Y-axis is cell number,
Curve one is cellular control unit growth curve, and curve two is 2 hour/day magnetic field radiation group cell growth curves, and curve three is 4 small
When/day magnetic field radiation group cell growth curve, curve four is 8 hour/day magnetic field radiation group cell growth curves；Right part of flg is
G401 cell suppression curve, wherein X-axis is magnetic field radiation number of days, and Y-axis is inhibition rate of tumor cell (%), and column one is 2 hours/day
Magnetic field radiation group inhibiting rate, column two are 4 hour/day magnetic field radiation group cell inhibitory rates, and column three is 8 hour/day magnetic field radiation groups
Cell inhibitory rate；* indicate there is significant difference compared with the control group；# indicates that comparison among groups have significant difference；Result is aobvious in figure
Show, the growth of magnetic field radiation group cell is suppressed, and the speed of growth slows down；Inhibiting rate extends with irradiation time and is risen, when irradiation
Between 8 hour/day groups 2 hours and 4 hour/day groups are significantly higher than to the inhibiting rate of cell.
Claims (1)
1. a kind of tumor suppression device of process control, which is characterized in that by AC power source (1), the first booster converter (2),
Second booster converter (3), first frequency concussion generator (4), second frequency shake generator (5), DC/AC all-wave inverter
(6), half-wave inverter (7), the first output element (8), the second output element (9), first group of coil (10), second group of coil
(11), temperature-monitoring element (12), the first magnetic field strength monitoring element (13), the second magnetic field strength monitoring element (14), temperature
Feedback element (15), the first magnetic field strength feedback element (16), the second magnetic field strength feedback element (17), central processing unit
(18), frequency setting element (19), magnetic field strength setting element (20), time set element (21), display (22), keyboard
(23), ventilation interface (24) and host computer (25)；AC power source (1) respectively with the first booster converter (2) and second liter
One end of buckling parallel operation (3) connects, and the other end of the first booster converter (2) and the second booster converter (3) is respectively with first
Frequency oscillation generator (4) and second frequency concussion generator (5) connection, first frequency shake generator (4) and second frequency
The other end of concussion generator (5) is connect with DC/AC all-wave inverter (6) and half-wave inverter (7) respectively, and DC/AC all-wave is inverse
The other end for becoming device (6) and half-wave inverter (7) is connect with the first output element (8) and the second output element (9) respectively, and first
The other end of output element (8) and the second output element (9) is connect with first group of coil (10) and second group of coil (11) respectively,
Between two groups of coils in irradiated area in be placed with temperature-monitoring element (12), the first magnetic field strength monitoring element (13) and
Second magnetic field strength monitoring element (14), temperature-monitoring element (12), the first magnetic field strength monitoring element (13) and the second magnetic field
Intensity monitoring element (14) respectively with temperature feedback element (15), the first magnetic field strength feedback element (16) and the second magnetic field strength
Feedback element (17) connection, temperature feedback element (15), the first magnetic field strength feedback element (16) and the second magnetic field strength feedback
The other end of element (17) is connect with central processing unit (18), and in addition rate of connections sets element to central processing unit (18)
(19), magnetic field strength setting element (20) and time setting element (21), the other end of frequency setting element (19) are separately connected
First frequency shakes generator (4) and second frequency concussion generator (5), and magnetic field strength sets element (20) and time setting member
The other end of part (21) is separately connected DC/AC all-wave inverter (6) and half-wave inverter (7), and central processing unit (18) in addition connects
Display (22) and keyboard (23) are connect, and is connected by ventilation interface (24) with host computer (25).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820793084.2U CN209270647U (en) | 2018-05-25 | 2018-05-25 | A kind of tumor suppression device of process control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820793084.2U CN209270647U (en) | 2018-05-25 | 2018-05-25 | A kind of tumor suppression device of process control |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209270647U true CN209270647U (en) | 2019-08-20 |
Family
ID=67597392
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820793084.2U Expired - Fee Related CN209270647U (en) | 2018-05-25 | 2018-05-25 | A kind of tumor suppression device of process control |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209270647U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108607162A (en) * | 2018-05-25 | 2018-10-02 | 浙江大学 | A kind of tumor suppression device of program control and use |
-
2018
- 2018-05-25 CN CN201820793084.2U patent/CN209270647U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108607162A (en) * | 2018-05-25 | 2018-10-02 | 浙江大学 | A kind of tumor suppression device of program control and use |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108607162A (en) | A kind of tumor suppression device of program control and use | |
| Blackman | Cell phone radiation: evidence from ELF and RF studies supporting more inclusive risk identification and assessment | |
| Henry et al. | The effect of magnetic fields on wound healing: experimental study and review of the literature | |
| US20030095022A1 (en) | Static magnetic field, method of creation, and resting surface therein | |
| US20150140633A1 (en) | System and method for proliferation of stem cells in cellular tissue | |
| US9757582B2 (en) | Tumor treatment with free radical lifetimes enhanced by persistant low strength magnetic field | |
| CN112007277A (en) | Pulsed electromagnetic field device for breast cancer treatment | |
| CN109596670A (en) | A kind of electric/magnetic field environment comprehensive generating system | |
| CN209270647U (en) | A kind of tumor suppression device of process control | |
| RU2106159C1 (en) | Method for selectively destroying cancer tumor cells | |
| CN106621050A (en) | Power frequency electromagnetic field generating device having tumor inhibiting effect and loading method | |
| Ahmed et al. | Design and development of an extremely low frequency (ELF) pulsed electromagnetic field (PEMF) system for wound healing promotion | |
| Shahbazi-Gahrouei et al. | Exposure of Extremely-Low Frequency (ELF) magnetic field may cause human cancer | |
| Babouri et al. | In vitro investigation of eddy current effect on pacemaker operation generated by low frequency magnetic field | |
| Lin et al. | Low-intensity pulsed ultrasound for killing tumor cells: the physical and biological mechanism | |
| JPS63283653A (en) | Apparatus for generating biologically active magnetic field and electromagnetic field | |
| Belik et al. | Magnetotherapy biotechnical system for rapid wound healing | |
| Tofani et al. | Anticancer activity by magnetic fields: inhibition of metastatic spread and growth in a breast cancer model | |
| Krawczyk et al. | New magnetotherapeutical devices experimental and simulation results | |
| Krstic et al. | The results of experimental exposition of mice by mobile telephones | |
| Ignatov et al. | Biological Effects of Christos Drossinakis on Model Bio Systems with Cancer Cells | |
| Miyamoto et al. | Effect of magnetic field at low frequency on cells arrangement | |
| CZ2004395A3 (en) | Numerically controlled pulse magneto-light therapeutic apparatus for surface therapy | |
| RU2090219C1 (en) | Electromagnetic device for carrying out biomedical studies and physiotherapy | |
| RU2676846C2 (en) | Method for pulsed electromagnetic exposure of cell cultures for medical or biological purposes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190820 Termination date: 20210525 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |