CN115109700A - Special cell culture dish of tumour treatment electric field basic experiment - Google Patents
Special cell culture dish of tumour treatment electric field basic experiment Download PDFInfo
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- CN115109700A CN115109700A CN202110283750.4A CN202110283750A CN115109700A CN 115109700 A CN115109700 A CN 115109700A CN 202110283750 A CN202110283750 A CN 202110283750A CN 115109700 A CN115109700 A CN 115109700A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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Abstract
The invention belongs to the field of cell experiments, in particular to a special cell culture dish for a Tumor treatment electric field (Tumor Treating fields) basic experiment, which comprises: culture dish lid, culture dish body, insulating plate electrode, metallic interconnect piece, culture dish lid is square with the culture dish body, be provided with a pair of insulating plate electrode that is parallel to each other on the inside bottom surface of culture dish body, distance between the insulating plate electrode can be according to the experiment required and adjust, insulating plate electrode is not connected a metallic interconnect piece separately with the one side of culture medium contact, the metallic interconnect piece is drawn forth from between culture dish lid and the culture dish body, connects in alternating electric field generating device's output. The cell culture dish can change the intensity of electric field by only adjusting the distance between the insulated electrode plates, so that the cell culture dish meets the requirement of TTfields basic experiment, and can also consider the air tightness of the culture dish when being connected with an external alternating electric field generating device, thereby ensuring the smooth operation of the experiment.
Description
Technical Field
The invention belongs to the field of cell experiments, and particularly relates to a special cell culture dish for an electric field basic experiment for tumor treatment.
Background
The culture dish is a laboratory vessel used for culturing microorganisms or cells, is usually round or square, consists of a dish bottom and a dish cover, and is generally made of glass or plastic. The culture dish is basically made of two types, mainly plastic and glass, and the glass can be used for plant materials, and can also be used for microorganism culture and animal cell adherent culture. The plastic may be of polyethylene material, disposable and reusable, suitable for laboratory inoculation, streaking, isolation of bacteria, and may also be used for the cultivation of plant material.
Tumor treatment electric Fields (Tumor Treating Fields TTFields) are an emerging anticancer means for inhibiting Tumor cell proliferation by interfering the mitosis of Tumor cells with sinusoidal alternating electric Fields of specific field strength (1-3V/cm) and specific frequency (100 KHz-300 KHz), and were originally proposed by professor Kirson in 2004, and in recent years, some large clinical trials prove that TTFields can safely and effectively prolong the life cycle of patients with glioblastoma, and the chinese national health commission included the brain glioma diagnosis and treatment standard in 2018.
The known method for adjusting the field intensity of the TTfields basic experiment is realized by changing the output voltage of the alternating electric field generating device, the frequency of the sinusoidal alternating electric field required by the TTfields is high, the difficulty in adjusting the output voltage of the alternating electric field generating device under the frequency is high, and the voltage adjusting equipment, namely a high-voltage amplifier, which can meet the requirement is expensive. In the electric field model generated by the TTFields basic experiment, the magnitude of the electric field between the two electrode plates is related to the distance between the two electrode plates, in addition to the output voltage of the alternating electric field generating device. Therefore, under the condition that the maximum output voltage is limited, the electric field intensity required by the TTfields basic experiment is met by adjusting the distance between the two parallel electrode plates, and the method is more economical and convenient. In addition, most of the conventional cell culture dishes are circular, and are limited in shape, so that it is difficult to adjust the distance to meet the requirement of the electric field intensity of the TTFields without changing the size of the metal electrode plate. The cell culture dish for the TTFields basic experiment needs to connect two insulated electrode plates arranged in the dish with the output end of the generating device of the alternating electric field outside the dish, and the tightness of the cell culture dish can be affected, so that the experiment fails.
Disclosure of Invention
The invention aims to provide a novel, convenient and economic TTfields special cell culture dish, which solves the problem of higher difficulty in adjusting the field intensity between insulating electrode plates by adjusting the output voltage of an alternating electric field generating device in the background art and also solves the problem of poor air tightness in the culture dish when the insulating electrode plates in the culture dish are connected with the output end of the alternating electric field generating device outside the culture dish.
The technical scheme of the invention is as follows: a special cell culture dish of TTfields basic experiment, characterized by includes: culture dish lid, culture dish body, insulating plate electrode, metal connecting piece, the culture dish lid is square with the culture dish body, be provided with a pair of insulating plate electrode that is parallel to each other on the inside bottom surface of the culture dish body, insulating plate electrode hugs closely and the inside bottom surface of the perpendicular to culture dish body is placed, distance between the insulating plate electrode can be according to experimenting required and adjusting, connect a metal connecting piece on a pair of insulating plate electrode separately, metal connecting piece draws forth from between culture dish lid and the culture dish body, connects in the output that can provide intermediate frequency alternating electric field generating device.
Preferably, the insulating electrode plate is formed by covering an insulating film outside the metal electrode plate.
Preferably, the insulating film of the insulated electrode plate has no toxic effect on cells.
Preferably, the metal electrode plate of the insulated electrode plate has good conductivity.
Preferably, the metal connecting sheet is connected to the outer side of the insulated electrode plate and is not in direct contact with the culture medium.
Preferably, the bottom surface of the outer part of the culture dish body is provided with a positioning scribed line, so that the distance between the insulating electrode plates can be conveniently set.
The invention has the beneficial effects that: the cell culture dish special for the TTfields basic experiment is designed by adopting a square culture dish, and the intensity of the electric field can be adjusted by only changing the distance between the insulating electrode plates under the condition that the output voltage of the medium-frequency alternating electric field generating device is limited, so that the requirement of the TTfields basic experiment is met; in addition, the invention also designs a new connection mode, so that the culture dish can be connected with an external alternating electric field generating device and simultaneously the air tightness of the culture dish is considered. In a word, the invention provides a simple, convenient, economical and practical cell culture dish special for TTfields basic experiments.
Drawings
FIG. 1 is a schematic view of the overall structure of a cell culture dish for TTfields basic experiments according to the present invention.
FIG. 2 is a schematic structural view of a cell culture dish body dedicated for TTfields basic experiments according to the present invention.
Fig. 3 is a schematic structural view of an insulated electrode plate of a cell culture dish for TTFields basic experiments according to the present invention.
In the figure: a culture dish cover-1, a culture dish body-2, a positioning reticle-201, an insulating electrode plate-3, an insulating film-301, a metal electrode plate-302 and a metal connecting sheet-4.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
Referring to fig. 1 to 3, a cell culture dish for TTFields basic experiments includes: the culture dish comprises a culture dish cover 1, a culture dish body 2, an insulating electrode plate 3 and a metal connecting sheet 4, wherein the culture dish cover 1 and the culture dish body 2 are square, the bottom surface of the culture dish body 2 is a square with the side length of 10cm, the culture dish cover 1 is a square slightly larger than the side length, and compared with a conventional round culture dish, the square culture dish has the advantages that when the distance between the insulating electrode plates 3 is changed according to the experimental requirement, the length of the insulating electrode plate 3 can be adjusted without cutting again, the insulating electrode plate 3 which is parallel to each other is arranged on the bottom surface inside the culture dish body 2, the insulating electrode plate 3 is tightly attached to and is placed perpendicular to the bottom surface inside the culture dish body 2, the distance between the two plates can be adjusted according to the experimental requirement, after the distance is adjusted, in order to prevent the culture medium from leaking outside, hot melt adhesive or other glue without cytotoxicity is used for reinforcing the contact place between the insulating electrode plate 3 and the culture dish body 2, the insulating electrode plates 3 are respectively connected with a metal connecting sheet 4, and the metal connecting sheets 4 are led out from between the culture dish cover 1 and the culture dish body 2 and are connected with the output end capable of providing a medium-frequency alternating electric field generating device.
Preferably, the insulated electrode plate 3 is formed by wrapping 301 an insulating film around a metal electrode plate 302.
Preferably, the insulating film 301 of the insulating electrode plate 3 is a heat-shrinkable film having no toxicity on cells, has an inner diameter of 11mm and a shrinkage ratio of 2:1, and can be completely attached to and cover a red copper electrode plate with the following specification after being heated.
Preferably, the metal electrode plate 302 of the insulated electrode plate is a copper plate with good conductivity according to the national standard T2, and the size specification of the copper plate is 94mm multiplied by 10mm multiplied by 1 mm.
Preferably, the optional lithium cell connection piece of pure nickel of metal connecting piece 4 has good electric conductivity and plasticity, and the connection piece is 0.1mm thick for the selection of this embodiment, connect in insulating electrode board 3 one side not contacting with the culture medium utilizes its frivolous, good characteristics of plasticity to draw it from between culture dish lid 1 and the culture dish body 2 simultaneously, hardly influences the original gas tightness of culture dish.
Preferably, the bottom surface of the outer part of the culture dish body 2 is provided with positioning scribed lines 201 every 1cm, so that the distance between the insulating electrode plates 3 can be conveniently set.
The invention is not the best known technology.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (6)
1. A special cell culture dish of tumour treatment electric field basic experiment, which is characterized by comprising: the culture dish is characterized by comprising a culture dish cover (1), a culture dish body (2), a pair of parallel insulating electrode plates (3) and a metal connecting sheet (4), wherein the culture dish cover (1) and the culture dish body (2) are square, the insulating electrode plates (3) are arranged on the bottom surface of the inside of the culture dish body (2), the insulating electrode plates (3) are tightly attached to the bottom surface of the inside of the culture dish body (2) and are perpendicular to the bottom surface of the inside of the culture dish body (2), the distance between the insulating electrode plates (3) can be adjusted according to the experiment, the metal connecting sheet (4) is connected to the insulating electrode plates (3), and the metal connecting sheet (4) is led out from the culture dish cover (1) and the culture dish body (2) and is connected to a generating device capable of providing an intermediate frequency alternating electric field.
2. A cell culture dish according to claim 1, wherein: the insulating electrode plate (3) is formed by an outer covering insulating film (301) and a metal electrode plate (302).
3. A cell culture dish according to claim 1, wherein: the insulating film (301) of the insulating electrode plate (3) has no toxic effect on cells.
4. A cell culture dish according to claim 1, wherein: the metal electrode plate (302) of the insulated electrode plate (3) has good conductivity.
5. A cell culture dish according to claim 1, wherein: the metal connecting sheet (4) is connected to the outer side of the insulated electrode plate (3) and is not in direct contact with the culture medium.
6. A cell culture dish according to claim 1, wherein: the positioning scribed lines (201) are drawn on the bottom surface of the outer part of the culture dish body (2), so that the distance between the insulating electrode plates (3) can be conveniently set.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110283750.4A CN115109700A (en) | 2021-03-17 | 2021-03-17 | Special cell culture dish of tumour treatment electric field basic experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110283750.4A CN115109700A (en) | 2021-03-17 | 2021-03-17 | Special cell culture dish of tumour treatment electric field basic experiment |
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| CN115109700A true CN115109700A (en) | 2022-09-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202110283750.4A Pending CN115109700A (en) | 2021-03-17 | 2021-03-17 | Special cell culture dish of tumour treatment electric field basic experiment |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116144491A (en) * | 2022-10-31 | 2023-05-23 | 湖南安泰康成生物科技有限公司 | Culture dish and culture dish system based on three-dimensional electric field |
-
2021
- 2021-03-17 CN CN202110283750.4A patent/CN115109700A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116144491A (en) * | 2022-10-31 | 2023-05-23 | 湖南安泰康成生物科技有限公司 | Culture dish and culture dish system based on three-dimensional electric field |
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