CN215440388U - Device for detecting enzymatic activity capacity - Google Patents
Device for detecting enzymatic activity capacity Download PDFInfo
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- CN215440388U CN215440388U CN202120954148.4U CN202120954148U CN215440388U CN 215440388 U CN215440388 U CN 215440388U CN 202120954148 U CN202120954148 U CN 202120954148U CN 215440388 U CN215440388 U CN 215440388U
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Abstract
The utility model relates to the technical field of detection devices, and discloses a device for detecting the enzymatic activity, which comprises an upper shell, a sample chamber, a flow guide pipe, a sample cover, a culture medium and a lifting device, wherein the upper shell is arranged in a box cover shape, the lower end of the upper shell is provided with a lower shell, the lower end of the upper shell is provided with a raised cylindrical plate, one side of the cylindrical plate is provided with gear teeth, the center of the upper end of the upper shell is provided with a circular through hole, and the upper end of the lower shell is provided with an annular groove. According to the utility model, the plurality of flow guide pipes are arranged at the lower end of the sample chamber, and the plurality of flow dividing pipes are arranged below the flow guide pipes, so that the bacillus fermentation liquid in the sample chamber uniformly flows into each culture medium in the downward flowing process, and the bottoms of the flow dividing pipes are positioned in the culture medium, so that the bacillus fermentation liquid is more fully contacted with the culture medium in the culture medium, and the size of a transparent ring in the culture medium is convenient to observe.
Description
Technical Field
The utility model relates to the technical field of detection devices, in particular to a device for detecting the enzymatic activity.
Background
The enzyme activity detector is a content measuring instrument for alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, lactate dehydrogenase, gamma glutamyltransferase, alpha hydroxybutyrate dehydrogenase, creatine kinase isozyme, cholinesterase and amylase in biological tissue samples, the existing enzyme detector is usually provided with an observation mirror to observe the state of the sample, but the position of the sample is often required to be adjusted in the process of placing the sample, the existing detector is required to repeatedly take the sample, the efficiency is very low, and impurities are easily stained in the taking process to influence the detection result.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a device for detecting the enzymatic activity, which can inject the same enzyme into different culture media and keep the conditions consistent in the using process.
In order to achieve the purpose, the utility model provides the following technical scheme: a device for detecting the activity of enzyme comprises an upper shell, a sample chamber, a flow guide pipe, a sample cover, a culture medium and a lifting device, wherein the upper shell is arranged in a box cover shape, the lower end of the upper shell is provided with a lower shell, the lower end of the upper shell is provided with a raised cylindrical plate, one side of the cylindrical plate is provided with wheel teeth, the center of the upper end of the upper shell is provided with a circular through hole, the upper end of the lower shell is provided with an annular groove, the annular plate at the lower end of the upper shell is positioned in the annular groove at the upper end of the lower shell, the sample chamber is positioned in the circular through hole at the upper end of the upper shell, the sample cover is positioned at the upper end of the sample chamber, the sample cover is fixedly connected with the sample chamber through threads, the lower end of the sample chamber is provided with a plurality of through holes, the flow guide pipe is fixed at the lower end of the sample chamber, and the inner wall of the flow guide pipe is communicated with the inner wall of the sample chamber through holes at the lower end of the sample chamber, the lower end of the guide pipe is connected with a plurality of shunt pipes, the culture medium is positioned in the lower end of the lower shell, the plurality of guide pipes are arranged at the lower end of the sample chamber, so that sample fermentation liquor in the sample chamber uniformly flows into the culture medium with different lower parts, and the activity of each enzyme in the fermentation liquor is preliminarily judged by laying a transparent ring in the culture medium.
Preferably, a plurality of grooves are formed in one side of the lower end of the lower shell, and the plurality of culture mediums are located in the grooves of the lower shell, so that the culture mediums containing various culture mediums can be taken out from the lower shell, and the size of the transparent ring in each culture medium can be observed conveniently.
Preferably, the lifting device comprises rotating wheels, a fixed pin, a planar helical gear, a rotating helical gear, a spring and a flat gear, wherein the two rotating wheels are positioned at two sides of the lower shell, a through hole is formed at the center of each rotating wheel, a fixed sleeve is arranged at the outer side of one end of each rotating wheel, the fixed sleeves are embedded on the outer wall of the lower shell, the planar helical gear is positioned inside the lower shell and fixed at one side of each rotating wheel, a through hole is formed at the center of each planar helical gear and is consistent with the through hole at the center of each rotating wheel, the rotating helical gear is positioned at one side of the planar helical gear, one end of each spring is fixed on the rotating helical gear, the other side of each spring is fixed on the lower shell, helical teeth in a circumferential array shape are arranged on the opposite surfaces of the planar helical gear and the rotating helical gear, so that the rotating wheels drive the planar helical gear to rotate, the plane bevel gear is matched with the rotary bevel gear to prevent the plane bevel gear from rotating reversely.
Preferably, the through hole is formed in the center of the flat gear and is consistent with the through hole in the center of the rotating wheel, the flat gear is located in an annular groove formed in the lower shell and located on one side of the spring, the through hole is formed in one side of the flat gear of the lower shell, the through hole in the lower shell is larger than the through hole in the center of the rotating wheel, the flat gear is matched with the gear teeth at the lower end of the upper shell, the flat gear is driven to rotate by the rotating wheel, the upper shell can be driven to move upwards, and the bottoms of the shunt tubes inside the upper shell are located at different positions of the culture medium conveniently.
Preferably, the center of the fixing pin is cylindrical, the two ends of the fixing pin are provided with raised direction columns, and the fixing pin is matched with the through holes on the rotating wheel, the plane helical gear and the flat gear, so that the fixing pin can rotate the wheel, the plane helical gear and the flat gear, and the plane helical gear and the flat gear are driven to rotate while the rotating wheel is rotated, and the upper shell is driven to move upwards.
Preferably, a through hole is formed in the center of the rotating bevel gear, the through hole in the center of the rotating bevel gear is larger than the through hole formed in the center of the plane bevel gear, spherical protrusions are arranged at two ends of the rotating bevel gear, the spherical protrusions at two ends of the rotating bevel gear are located in cylindrical grooves formed in the outer wall of the rotating bevel gear of the lower shell, the rotating bevel gear is clamped inside the lower shell through the spherical protrusions at two sides of the rotating bevel gear, the rotating bevel gear compresses the spring to move left and right, and rotation of the plane bevel gear is facilitated.
Preferably, bacillus fermentation liquid is placed in the sample chamber, casein fixed culture mediums, starch as a carbon source solid culture medium and microcrystalline cellulose fixed culture mediums are respectively placed in the culture mediums, so that the bacillus fermentation liquid in the sample chamber can rapidly react with the casein fixed culture medium, the starch as a carbon source solid culture medium and the microcrystalline cellulose fixed culture medium in the culture mediums, and the activity of each enzyme in the fermentation liquid is judged according to the size of the transparent ring.
The utility model provides a device for detecting the enzymatic activity. The method has the following beneficial effects:
(1) according to the utility model, the plurality of flow guide pipes are arranged at the lower end of the sample chamber, and the plurality of flow dividing pipes are arranged below the flow guide pipes, so that the bacillus fermentation liquid in the sample chamber uniformly flows into each culture medium in the downward flowing process, and the bottoms of the flow dividing pipes are positioned in the culture medium, so that the bacillus fermentation liquid is more fully contacted with the culture medium in the culture medium, and the size of a transparent ring in the culture medium is convenient to observe.
(2) The lifting device is arranged, so that the plane bevel gear and the plane gear rotate by limiting the fixed pin to the rotating wheel, the plane bevel gear and the plane gear in the rotating process of the rotating wheel through rotating the rotating wheel, and the plane bevel gear extrudes the rotating bevel gear in the rotating process to match the plane bevel gear with the rotating bevel gear through the helical teeth in the circumferential array, so that the reverse rotation of the plane gear is prevented.
Drawings
FIG. 1 is a cross-sectional view of the present invention;
FIG. 2 is a partial cross-sectional view of the lift device of the present invention;
fig. 3 is a perspective view of a rotating helical gear of the present invention.
In the figure: 1. an upper housing; 2. a lower housing; 3. a sample chamber; 4. a flow guide pipe; 5. a shunt tube; 6. a sample cover; 7. a culture medium; 8. a lifting device; 801. a rotating wheel; 802. a fixing pin; 803. fixing a sleeve; 804. a planar helical gear; 805. rotating the bevel gear; 806. a spring; 807. a flat gear.
Detailed Description
As shown in fig. 1-3, the present invention provides a technical solution: a device for detecting the activity of enzyme comprises an upper shell 1, a sample chamber 3, a flow guide pipe 4, a sample cover 6, a culture medium 7 and a lifting device 8, wherein the upper shell 1 is arranged in a box cover shape, the lower end of the upper shell is provided with a lower shell 2, the lower end of the upper shell 1 is provided with a raised cylindrical plate, one side of the cylindrical plate is provided with gear teeth, the center of the upper end of the upper shell 1 is provided with a circular through hole, the upper end of the lower shell 2 is provided with an annular groove, the annular plate at the lower end of the upper shell 1 is positioned in the annular groove at the upper end of the lower shell 2, the sample chamber 3 is positioned in the circular through hole at the upper end of the upper shell 1, the sample cover 6 is positioned at the upper end of the sample chamber 3, the sample cover 6 is fixed with the sample chamber 3 through threaded connection, the lower end of the sample chamber 3 is provided with a plurality of through holes, the flow guide pipe 4 is fixed at the lower end of the sample chamber 3, and the inner wall of the flow guide pipe 4 is communicated with the inner wall of the sample chamber 3 through holes at the lower end of the sample chamber 3, the lower end of the guide pipe 4 is connected with a plurality of shunt pipes 5, a plurality of culture mediums 7 are positioned in the lower end of the lower shell 2, the plurality of guide pipes 4 are arranged at the lower end of the sample chamber 3, so that the sample fermentation liquor in the sample chamber 3 uniformly flows into the culture mediums with different lower portions, and the activity of each enzyme in the fermentation liquor is preliminarily judged by arranging a transparent ring in the culture mediums. A plurality of grooves are formed in one side of the lower end of the lower shell 2, and a plurality of culture mediums 7 are located in the grooves of the lower shell 2, so that the culture mediums 7 containing various culture mediums can be taken out from the lower shell 2, and the size of a transparent ring in each culture medium can be observed conveniently.
The lifting device 8 comprises a rotating wheel 801, a fixed pin 802, a plane bevel gear 804, a rotating bevel gear 805, a spring 806 and a flat gear 807, wherein the two rotating wheels 801 are positioned at two sides of the lower shell 2, a through hole is arranged at the center of the rotating wheel 801, a fixed sleeve 803 is arranged at the outer side of one end of the rotating wheel 801, the fixed sleeve 803 is embedded on the outer wall of the lower shell 2, the plane bevel gear 804 is positioned inside the lower shell 2 and is fixed at one side of the rotating wheel 801, a through hole is arranged at the center of the plane bevel gear 804 and is consistent with the through hole at the center of the rotating wheel 801, the rotating bevel gear 805 is positioned at one side of the plane bevel gear 804, one ends of a plurality of springs 806 are fixed on the rotating bevel gear 805, the other side of the springs 806 is fixed on the lower shell 2, and the opposite surfaces of the plane bevel gear 804 and the rotating bevel gear 805 are both provided with helical teeth in a circumferential array shape, so that the rotating wheel 801 drives the plane bevel gear 804 to rotate, the bevel gear flat 804 is prevented from rotating in the reverse direction by the bevel gear flat 804 cooperating with the bevel gear rotating 805. The through hole has been seted up at the spur gear 807 center department to keep unanimous with the through hole of rotating wheel 801 center department, spur gear 807 is located the annular groove of seting up in casing 2 down, and be located one side of spring 806, casing 2 has seted up the through hole in one side of spur gear 807 down, and the through hole on casing 2 is greater than the through hole of rotating wheel 801 center department down, spur gear 807 and the teeth of a cogwheel that upper casing 1 is located the lower extreme cooperate, make spur gear 807 drive the rotation by rotating wheel 801 after, can drive upper casing 1 and upwards remove, be convenient for the bottom of the shunt tubes 5 of upper casing 1 inside is located the different positions of culture medium 7. The center of the fixing pin 802 is cylindrical, and the two ends of the fixing pin 802 are provided with raised direction columns, the fixing pin 802 is matched with through holes on the rotating wheel 801, the plane helical gear 804 and the flat gear 807, so that the fixing pin 802 communicates the rotating wheel 801, the plane helical gear 804 and the flat gear 807, and the plane helical gear 804 and the flat gear 807 are driven to rotate while the rotating wheel 801 is rotated, so as to drive the upper shell 1 to move upwards. A through hole is formed in the center of the rotating bevel gear 805, the through hole in the center of the rotating bevel gear 805 is larger than the through hole formed in the center of the planar bevel gear 804, spherical protrusions are arranged at two ends of the rotating bevel gear 805, the spherical protrusions at the two ends of the rotating bevel gear 805 are located in cylindrical grooves formed in the outer wall of the rotating bevel gear 805 of the lower shell 2, the rotating bevel gear 805 is clamped inside the lower shell 2 through the spherical protrusions at the two sides of the rotating bevel gear 805, the rotating bevel gear 805 compresses the spring 806 to move left and right, and rotation of the planar bevel gear 804 is facilitated.
The bacillus fermentation liquid is placed in the sample chamber 3, casein fixed culture mediums, starch used as a carbon source solid culture medium and a microcrystalline cellulose fixed culture medium are respectively placed in the plurality of culture mediums 7, so that the bacillus fermentation liquid in the sample chamber 3 can rapidly react with the casein fixed culture medium, the starch used as the carbon source solid culture medium and the microcrystalline cellulose fixed culture medium in the culture mediums 7, and the activity of each enzyme in the fermentation liquid is judged according to the size of the transparent ring.
When in use, a culture medium 7 with various culture mediums is placed in the lower shell 2, the shunt pipe 5 is fixed in the culture medium 7, the bottom of the shunt pipe 5 is positioned in the culture medium of the culture medium 7, then the rotating wheel 801 is adjusted, in the adjusting process of the rotating wheel 801, the fixed pin 802 drives the plane bevel gear 804 and the plane bevel gear 807 to rotate, the plane bevel gear 804 rotates and presses the rotating bevel gear 805, the spring 806 behind the rotating bevel gear 805 is pressed to contract, after the spring 806 contracts, the rotating bevel gear 805 is driven to move, the plane bevel gear 804 rotates, after the rotating wheel 801 stops rotating, the rotating bevel gear 805 limits the plane bevel gear 804, the plane bevel gear 804 is fixed, in the rotating process of the rotating wheel 801, the plane gear 807 rotates, the plane gear 807 is matched with the gear teeth at the lower end of the upper shell 1, so as to drive the upper shell 1 to move upwards, and then the height of the shunt pipe 5 in the culture medium 7 is adjusted, so that the accuracy of each test is ensured.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and equivalent alternatives or modifications according to the technical solution of the present invention and the inventive concept thereof should be covered by the scope of the present invention.
Claims (7)
1. The utility model provides a detect device of enzyme activity ability size, includes upper housing (1), sample room (3), honeycomb duct (4), sample lid (6), culture medium (7), elevating gear (8), its characterized in that: the upper shell (1) is arranged in a box cover shape, the lower end of the upper shell is provided with a lower shell (2), the lower end of the upper shell (1) is provided with a raised cylindrical plate, one side of the cylindrical plate is provided with gear teeth, the center of the upper end of the upper shell (1) is provided with a circular through hole, the upper end of the lower shell (2) is provided with an annular groove, the annular plate at the lower end of the upper shell (1) is positioned in the annular groove at the upper end of the lower shell (2), the sample chamber (3) is positioned in the circular through hole at the upper end of the upper shell (1), the sample cover (6) is positioned at the upper end of the sample chamber (3), the sample cover (6) is fixed with the sample chamber (3) through threaded connection, the lower end of the sample chamber (3) is provided with a plurality of through holes, the flow guide pipe (4) is fixed at the lower end of the sample chamber (3), and the inner wall of the flow guide pipe (4) is communicated with the inner wall of the sample chamber (3) through the through holes at the lower end of the sample chamber (3), the lower end of the draft tube (4) is connected with a plurality of shunt tubes (5), and the culture medium (7) is positioned in the lower end of the lower shell (2).
2. The device for detecting the activity of an enzyme according to claim 1, wherein: a plurality of grooves are formed in one side of the lower end of the lower shell (2), and the plurality of culture mediums (7) are located in the grooves of the lower shell (2).
3. The device for detecting the activity of an enzyme according to claim 1, wherein: the lifting device (8) comprises rotating wheels (801), fixing pins (802), a planar helical gear (804), rotating helical gears (805), springs (806) and flat gears (807), wherein the two rotating wheels (801) are positioned at two sides of the lower shell (2), a through hole is formed in the center of each rotating wheel (801), a fixing sleeve (803) is arranged on the outer side of one end of each rotating wheel (801), the fixing sleeve (803) is embedded on the outer wall of the lower shell (2), the planar helical gear (804) is positioned in the lower shell (2) and fixed on one side of each rotating wheel (801), a through hole is formed in the center of each planar helical gear (804) and is consistent with the through hole in the center of each rotating wheel (801), the rotating helical gears (805) are positioned on one side of the planar helical gears (804), and one ends of a plurality of the springs (806) are fixed on the rotating helical gears (805), and the other side of the spring (806) is fixed on the lower shell (2), and the opposite surfaces of the plane helical gear (804) and the rotary helical gear (805) are respectively provided with helical teeth in a circumferential array.
4. The device for detecting the activity of an enzyme according to claim 3, wherein: the center of the flat gear (807) is provided with a through hole and is consistent with the through hole in the center of the rotating wheel (801), the flat gear (807) is positioned in an annular groove formed in the lower shell (2) and is positioned on one side of the spring (806), the lower shell (2) is provided with a through hole on one side of the flat gear (807), the through hole on the lower shell (2) is larger than the through hole in the center of the rotating wheel (801), and the flat gear (807) is matched with the gear teeth of the upper shell (1) positioned at the lower end.
5. The device for detecting the activity of an enzyme according to claim 4, wherein: the center of the fixing pin (802) is cylindrical, the two ends of the fixing pin are provided with raised direction columns, and the fixing pin (802) is matched with through holes in the rotating wheel (801), the plane helical gear (804) and the plane gear (807).
6. The device for detecting the activity of an enzyme according to claim 5, wherein: the center of the rotating bevel gear (805) is provided with a through hole, the through hole at the center of the rotating bevel gear (805) is larger than the through hole at the center of the plane bevel gear (804), spherical bulges are arranged at two ends of the rotating bevel gear (805), and the spherical bulges at two ends of the rotating bevel gear (805) are positioned in a cylindrical groove which is arranged on the outer wall of the rotating bevel gear (805) of the lower shell (2).
7. The device for detecting the activity of an enzyme according to claim 1, wherein: bacillus fermentation liquor is placed in the sample chamber (3), casein fixed culture media, starch as a carbon source solid culture medium and microcrystalline cellulose fixed culture media are respectively placed in the plurality of culture media (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120954148.4U CN215440388U (en) | 2021-05-07 | 2021-05-07 | Device for detecting enzymatic activity capacity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120954148.4U CN215440388U (en) | 2021-05-07 | 2021-05-07 | Device for detecting enzymatic activity capacity |
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| Publication Number | Publication Date |
|---|---|
| CN215440388U true CN215440388U (en) | 2022-01-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120954148.4U Active CN215440388U (en) | 2021-05-07 | 2021-05-07 | Device for detecting enzymatic activity capacity |
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|---|---|
| CN (1) | CN215440388U (en) |
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2021
- 2021-05-07 CN CN202120954148.4U patent/CN215440388U/en active Active
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