CN215375258U - Liquid workstation for solid phase extraction and chip array sample application - Google Patents
Liquid workstation for solid phase extraction and chip array sample application Download PDFInfo
- Publication number
- CN215375258U CN215375258U CN202120299322.6U CN202120299322U CN215375258U CN 215375258 U CN215375258 U CN 215375258U CN 202120299322 U CN202120299322 U CN 202120299322U CN 215375258 U CN215375258 U CN 215375258U
- Authority
- CN
- China
- Prior art keywords
- module
- positive pressure
- solid phase
- phase extraction
- nitrogen blowing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The invention relates to the field of biology, in particular to a liquid workstation for solid phase extraction and chip array sample application, which comprises an L-shaped working platform, a plurality of working disk positions arranged on the horizontal plane of the working platform and a disk moving claw for grabbing the working disk positions, wherein each working disk position comprises a positive pressure module, a nitrogen blowing module, a gun head placing module, a product collecting module, a sample application module, a reagent storage module, a solid phase extraction module, an oscillation temperature control module and a sample module. The invention can be used in the fields of clinical examination, scientific research, blood stations and the like, realizes automation and convenience of experiments, reduces the working intensity of inspectors, greatly reduces misoperation of the inspectors, and is convenient for same management of data and the like. The workstation has wide application and can be used in the fields of scientific research, medical treatment and the like.
Description
Technical Field
The invention relates to the field of biology, in particular to a liquid workstation for solid phase extraction and chip array spotting.
Background
With the development of immunological and molecular biological techniques, the concept of biomarkers has been proposed. A marker related to cell growth and proliferation not only can discuss pathogenesis from molecular level, but also has unique advantages in accurately and sensitively evaluating early and low-level damage of organisms, provides early warning for diseases, and provides a basis for auxiliary diagnosis for clinicians to a great extent. The biomarker is closely related to diseases, has a huge prospect in diagnosis, and has the advantages of simple and convenient detection, no pain and the like compared with the detection method used clinically at present, and the biomarker related to the diseases is enriched in a human body for diagnosis.
The research and diagnosis using biomarkers is a complex and tedious process, and the processing includes extraction, purification, drying, spotting, liquid chromatography, and flight mass spectrometry. When the number of samples is continuously increased, a lot of time is consumed by a plurality of laboratory workers to complete the whole process, and the researchers are in contact with chemicals for a long time and are harmful to health. Traditional manual operations have not been able to meet high throughput, rapid processing, and full or partial automation of the experimental process is the wind direction in which this application is developed.
Therefore, in view of the above situation, there is a need to develop a liquid workstation for solid phase extraction and chip array spotting to overcome the shortcomings of the current practical application.
Disclosure of Invention
The present invention is directed to a liquid station for solid phase extraction and chip array spotting, which solves the above problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a liquid work station for solid phase extraction and chip array spotting, includes the work platform of L type, a plurality of work stations that set up on the work platform horizontal plane and move a set claw that snatchs work stations, work stations places module, product collection module, spotting module, reagent storage module, solid phase extraction module, oscillation temperature control module and sample module including malleation module, nitrogen blow module, rifle head, wherein:
the positive pressure module comprises a positive pressure head main part and a positive pressure head seat and is used for pressurizing the SPE column, the lower part of the positive pressure head main part is fixedly connected with the positive pressure head seat, and the upper end and the lower end of the positive pressure head seat are open and hollow; the upper part of the positive pressure head main part is fixedly provided with an elbow joint, one end of the elbow joint is communicated with a pipeline, the other end of the elbow joint is communicated with the positive pressure head main part, and the pipeline is communicated with an air compressor; a positive pressure sealing ring and a sealing ring fixing plate are arranged between the positive pressure head main part and the positive pressure head seat, and a plurality of through holes for gas to flow are formed in the sealing ring fixing plate;
the nitrogen blowing module comprises a nitrogen blowing seat and a plurality of nitrogen blowing needles fixedly arranged at the bottom of the nitrogen blowing seat, the nitrogen blowing seat is hollow, the nitrogen blowing needles are communicated with the nitrogen blowing seat, a sealing cover is arranged at the top of the nitrogen blowing seat, and the side edge of the nitrogen blowing seat is communicated with a nitrogen quick connector;
a temperature control adapter is arranged below the product collecting module, a collecting pipe frame is arranged above the product collecting module, the collecting pipe frame is not limited to a test tube and a centrifuge tube, and an SPE column and a nitrogen blowing module can be respectively stacked on the collecting pipe frame;
the collection pipe frame comprises an SPE column frame, and an SPE collection pipe for storing SPE columns is arranged in the SPE column frame;
the sample application module comprises a small bottle adapter arranged on the left side and a chip adapter arranged on the right side and used for fixing a chip, and a temperature control adapter is arranged at the bottom of the chip adapter;
the reagent storage module comprises a heat-insulating shell and a semiconductor refrigeration piece arranged at the bottom of the heat-insulating shell, a cold conduction plate is arranged between the heat-insulating shell and the semiconductor refrigeration piece, and an upper refrigeration adapter for storing a plurality of containers is arranged at the upper part of the heat-insulating shell;
the oscillation temperature control module comprises an oscillator upper plate, an eccentric shaft and an oscillation motor, a host connecting plate is fixedly installed below the oscillator upper plate, the oscillation motor is fixedly installed at the bottom of the host connecting plate through an oscillation motor base, and the output end of the oscillation motor is fixedly connected with the eccentric shaft.
As a further scheme of the invention: two pin fixed plates I are symmetrically installed on the left side and the right side of the upper portion of the positive pressure head main part, and a plurality of pins I used for fixing the positive pressure head main part are fixedly installed on the pin fixed plates I.
As a further scheme of the invention: the nitrogen gas quick-operation joint is communicated with a nitrogen gas bottle, and the number of the nitrogen blowing needles is 12.
As a further scheme of the invention: and a nitrogen blowing sealing ring is arranged at the joint of the nitrogen blowing seat and the sealing cover.
As a further scheme of the invention: a fan support is arranged below the semiconductor refrigeration piece, a fan is mounted on the fan support, and a copper radiator and a heat conduction plate are further arranged between the semiconductor refrigeration piece and the fan.
As a further scheme of the invention: the upper plate of the oscillator is connected with a collecting pipe adapter, and a plurality of collecting pipes driven by the eccentric shaft to oscillate are placed on the collecting pipe adapter; the collecting pipe adapter is connected with the upper plate of the oscillator through a pin fixing plate II.
As a further scheme of the invention: and a balance block and a return spring which are matched with the eccentric shaft are also arranged at the joint of the output end of the oscillating motor and the eccentric shaft.
As a further scheme of the invention: the sample module comprises a sample frame lower plate and a sample frame upper plate, wherein the sample frame lower plate and the sample frame upper plate are fixedly connected through a support column.
As a further scheme of the invention: the positive pressure module, the nitrogen blowing module, the gun head placing module, the product collecting module, the sample application module, the reagent storage module, the solid phase extraction module, the oscillation temperature control module and the sample module are arranged in a Sudoku mode.
As a further scheme of the invention: and the mechanical arm assembly is arranged on the vertical surface of the working platform and used for driving the tray moving claw to perform position adjustment, and comprises an x-axis mechanical arm for driving the tray moving claw to move along an x axis, a y-axis mechanical arm for driving the tray moving claw to move along a y axis and a z-axis mechanical arm for driving the tray moving claw to move along a z axis.
Compared with the prior art, the invention has the beneficial effects that: the invention can be used in the fields of clinical examination, scientific research, blood stations and the like, takes the specific biomarkers of human bodies as samples, including but not limited to saliva, serum, urine and other samples, to carry out rapid biomarker extraction and purification, and automatically complete product drying and sample application, and the products processed by the liquid workstation can be used for liquid chromatography and flight mass spectrometry detection. In addition, the biomarker solid-phase extraction, the product nitrogen blowing and drying and the sample application platform can realize automation and convenience of experiments, reduce the working strength of inspectors, greatly reduce misoperation of the inspectors, facilitate the same management of data and the like. The workstation has wide application and can be used in the fields of scientific research, medical treatment and the like.
Drawings
FIG. 1 is a schematic diagram of a liquid station for solid phase extraction and chip array spotting.
FIG. 2 is a schematic diagram of the structure of the working platform in the liquid working station for solid phase extraction and chip array spotting.
FIG. 3 is a schematic diagram of the structure of the tray-moving claw in the liquid working station for solid phase extraction and chip array spotting.
FIG. 4 is a schematic diagram of a positive pressure module in a liquid workstation for solid phase extraction and chip array spotting.
FIG. 5 is a schematic diagram of a nitrogen blowing module in a liquid workstation for solid phase extraction and chip array spotting.
FIG. 6 is a schematic diagram of the collection tube rack in the liquid station for solid phase extraction and chip array spotting.
FIG. 7 is a schematic diagram of a reagent storage module in a liquid workstation for solid phase extraction and chip array spotting.
FIG. 8 is a schematic diagram of a solid phase extraction module in a liquid workstation for solid phase extraction and chip array spotting.
FIG. 9 is a schematic diagram of the structure of the sample module in the liquid workstation for solid phase extraction and chip array spotting.
In the figure: 1-working platform, 101-emergency stop button, 102-pressure regulating valve, 103-x axis mechanical arm, 104-y axis mechanical arm, 105-z axis mechanical arm, 2-air pump, 3-tray moving claw, 31-outer cover, 32-rotating motor, 33-rotating motor seat, 34-rack, 35-guide rail, 36-clamping piece, 37-main machine seat, 4-positive pressure module, 41-positive pressure head main part, 42-pin I, 43-pin fixing plate I, 44-elbow joint, 45-positive pressure sealing ring, 46-sealing ring fixing plate, 47-positive pressure head seat, 5-nitrogen blowing module, 51-nitrogen blowing seat, 52-nitrogen blowing needle, 53-nitrogen blowing sealing ring, 54-nitrogen quick joint, 55-sealing cover, 6-gun head placing module, 7-product collecting module, 8-sample application module, 9-reagent storage module, 901-fan, 902-fan bracket, 903-copper radiator, 904-heat conducting plate, 905-semiconductor refrigerating plate, 906-wire harness frame, 907-upper refrigerating adapter, 908-cold conducting plate, 909-heat preservation shell, 911, temperature sensor, 10-solid phase extraction module, 1001-oscillating motor, 1002-oscillating motor base, 1003-bearing base, 1004-return spring, 1005-eccentric shaft, 1006-balance block, 1007-main machine connecting plate, 1008-heat insulation plate, 1009-heating plate, 1010-oscillator upper plate, 1011-pin fixing plate II, 1012-collecting pipe adapter, 1013-pin II, 9-sample application module, 9-reagent storage module, 909-cold conducting plate, 909-heat preservation shell, 911-temperature sensor, 10-solid phase extraction module, 1001-oscillating motor, 1002-oscillating motor, 1003-bearing base, 1004-return spring, 1005-eccentric shaft, 1006-balance block, 1007-main machine connecting plate, 1008-heat insulation plate, 1009-heating plate, 1010-oscillator upper plate II, 1011-pin fixing plate II, 1012-collecting pipe adapter, 1013-pin II, and others, 1014-collection pipe, 11-oscillation temperature control module, 12-sample module, 1201-sample rack lower plate, 1202-support column, 1203-sample rack upper plate, 1204-sample test tube, 13-waste liquid level module, 14-gun head waste groove, 15-collection pipe rack, 1501-SPE column rack, 1502-SPE column and 1503-SPE collection pipe.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
Reference will now be made in detail to embodiments of the present patent, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present patent and are not to be construed as limiting the present patent.
In the description of this patent, it is to be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the patent and for the simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the patent.
In the description of this patent, it is noted that unless otherwise specifically stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can include, for example, fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meaning of the above terms in this patent may be understood by those of ordinary skill in the art as appropriate.
Referring to fig. 1-9, in an embodiment of the present invention, a liquid workstation for solid phase extraction and chip array spotting includes an L-shaped working platform 1, a plurality of working stations disposed on a horizontal plane of the working platform 1, and a tray moving claw 3 configured to grasp the working stations, where the working stations include a positive pressure module 4, a nitrogen blowing module 5, a gun head placing module 6, a product collecting module 7, a spotting module 8, a reagent storage module 9, a solid phase extraction module 10, an oscillation temperature control module 11, and a sample module 12, where:
the positive pressure module 4 comprises a positive pressure head main part 41 and a positive pressure head seat 47 and is used for pressurizing the SPE column, the lower part of the positive pressure head main part 41 is fixedly connected with the positive pressure head seat 47, and the upper end and the lower end of the positive pressure head seat 47 are open and the interior of the positive pressure head seat 47 is hollow; an elbow joint 44 is fixedly installed on the upper part of the positive pressure head main part 41, one end of the elbow joint 44 is communicated with a pipeline, the other end of the elbow joint 44 is communicated with the positive pressure head main part 41, and the pipeline is communicated with an air compressor; a positive pressure sealing ring 45 and a sealing ring fixing plate 46 are arranged between the positive pressure head main part 41 and the positive pressure head seat 47, a plurality of through holes for gas to flow are formed in the sealing ring fixing plate 46, the air compressor is used for ventilating the interior of the pipeline, the gas is sent into the positive pressure head main part 41 and the positive pressure head seat 47 along the pipeline and the elbow joint 44, and the SPE column is pressurized and used for controlling the flow rate of SPE column activation, sample loading, washing and elution;
in the embodiment of the present invention, the SPE column is a solid-phase extraction column, and is a sample pretreatment device for extraction, separation, and concentration developed from a chromatography column.
In the embodiment of the present invention, the elbow joint 44 is provided with a three-way valve, the three-way valve is tightly attached to the SPE column through a positive pressure head, an air compressor generates power, and the three-way valve adjusts and controls the speed of the liquid flowing out of the solid phase extraction column;
in the embodiment of the present invention, it should be noted that the number of positive pressure head channels of the positive pressure module 4 is designed according to the number of samples, and preferably, the number of positive pressure head channels is four;
furthermore, in the embodiment of the present invention, two pin fixing plates i are symmetrically installed on the left and right sides of the upper portion of the positive indenter main part 41, and a plurality of pins i 42 for fixing the positive indenter main part 41 are fixedly installed on the pin fixing plates i.
The nitrogen blowing module 5 comprises a nitrogen blowing seat 51 and a plurality of nitrogen blowing needles 52 fixedly arranged at the bottom of the nitrogen blowing seat 51, the nitrogen blowing seat 51 is hollow, the nitrogen blowing needles 52 are communicated with the nitrogen blowing seat 51, a sealing cover 55 is arranged at the top of the nitrogen blowing seat 51, a nitrogen quick connector 54 is communicated with the side edge of the nitrogen blowing seat 51, the nitrogen quick connector 54 is used for ventilating the interior of the nitrogen blowing seat 51, and gas is released by the nitrogen blowing needles 52 to dry a sample;
in the embodiment of the present invention, the nitrogen quick connector 54 is communicated with a nitrogen gas cylinder, the number of the nitrogen blow pins 52 is set according to the number of the samples, preferably, the nitrogen blow mold block 5 is 12 channels, and it is understood that the number of the nitrogen blow pins 52 is 12;
further, in the embodiment of the present invention, a nitrogen-blowing sealing ring 53 is disposed at a connection position of the nitrogen-blowing seat 51 and the sealing cover 55, for improving the sealing performance of the nitrogen-blowing seat 51.
A gun head adapting groove is formed in the gun head placing module 6, and gun heads with the specifications of 1000, 200, 100 and 50 mu L can be placed according to different experiments. Preferably, the lance tip placement module 6 is configured with lance tip adaptation slots of 1000 μ L and 50 μ L specifications as required by the entire experiment.
A temperature control adapter is arranged below the product collecting module 7 and used for accelerating nitrogen blow drying, a collecting pipe frame 15 is arranged above the product collecting module, the collecting pipe frame 15 is not limited to a test tube and a centrifuge tube, and an SPE column and a nitrogen blow molding block 5 can be respectively stacked on the collecting pipe frame 15 and are respectively used for containing a purified product and drying;
in the embodiment of the invention, preferably, in order to prevent the product from being stained on the wall of the glass test tube and causing loss in the drying process, 12 centrifuge tubes are placed in the collection frame, and the temperature setting temperature of the temperature control adapter is 80 ℃;
The sample application module 8 comprises a small bottle adapter arranged on the left side and a chip adapter arranged on the right side and used for fixing a chip, and a temperature control adapter is arranged at the bottom of the chip adapter and used for quickly drying after sample application;
in one embodiment of the present invention, the vial adapter holds 12 liquid phase vials, the chip adapter holds a MADLI chip, and the temperature-controlled adapter sets a temperature of 80 ℃.
The reagent storage module 9 comprises a heat preservation shell 909 and a semiconductor refrigeration sheet 905 arranged at the bottom of the heat preservation shell 909, a cold conduction plate 908 is arranged between the heat preservation shell 909 and the semiconductor refrigeration sheet 905, and an upper refrigeration adapter 907 for storing a plurality of containers is arranged at the upper part of the heat preservation shell 909;
in the embodiment of the present invention, a temperature sensor 911 for measuring temperature is further installed on the thermal insulation housing 909;
in another embodiment of the present invention, a fan bracket 902 is disposed below the semiconductor refrigeration sheet 905, a fan 901 is mounted on the fan bracket 902, a copper radiator 903 and a heat conducting plate 904 are further disposed between the semiconductor refrigeration sheet 905 and the fan 901, heat generated in the refrigeration process of the semiconductor refrigeration sheet 905 is conducted to the heat conducting plate 904, and is dissipated by the copper radiator 903 and the fan 901.
The oscillation temperature control module 11 comprises an oscillator upper plate 1010, an eccentric shaft 1005 and an oscillation motor 1001, a main machine connecting plate 1007 is fixedly installed below the oscillator upper plate 1010, the oscillation motor 1001 is fixedly installed at the bottom of the main machine connecting plate 1007 through an oscillation motor base 1002, and the output end of the oscillation motor 1001 is fixedly connected with the eccentric shaft 1005;
in the embodiment of the invention, the upper plate 1010 of the oscillator is connected with a collecting pipe adapter 1012, and a plurality of collecting pipes 1014 driven by an eccentric shaft 1005 to oscillate are placed on the collecting pipe adapter 1012; the collecting pipe adapter 1012 is connected with the upper plate 1010 of the oscillator through a pin fixing plate II 1011;
in another embodiment of the present invention, a balance weight 1006 and a return spring 1004 adapted to the eccentric shaft 1005 are further disposed at a connection between the output end of the oscillating motor 1001 and the eccentric shaft 1005;
a certain number of collecting pipes 1014 are fixed by a collecting pipe adapter 1012 and used for the reaction of a sample and various solutions, an oscillation temperature control module 11 provides temperature and oscillation for the reaction of the sample, the rapid rise and fall of the temperature in the experimental process are realized, the reaction time is shortened, preferably, 12 collecting pipes 1014 with 2mL are fixed by the collecting pipe adapter 1012, the sample is incubated by oscillation at the temperature of 65 ℃ in the experiment, the protein is denatured at high temperature, the molecular structure is loose, then the temperature is reduced, and protease is added at a proper temperature for incubation by oscillation to complete the enzymolysis reaction.
The sample module 12 includes a lower sample rack plate 1201 and an upper sample rack plate 1203, the lower sample rack plate 1201 and the upper sample rack plate 1203 are connected and fixed by a support column 1202 for placing a sample test tube 1204.
It should be noted that, in the embodiment of the present invention, the components on the work tray include a positive pressure module 4, a nitrogen blowing module 5, a gun head placing module 6, a product collecting module 7, a spotting module 8, a reagent storage module 9, a solid phase extraction module 10, an oscillation temperature control module 11, and a sample module 12, the number of which can be increased or decreased according to the application, and the positive pressure module 4, the nitrogen blowing module 5, the gun head placing module 6, the product collecting module 7, the spotting module 8, the reagent storage module 9, the solid phase extraction module 10, the oscillation temperature control module 11, and the sample module 12 are arranged in a grid manner, so that the work areas are relatively and independently separated, mutual pollution of the sample area, the reagent area, the solid phase extraction area, and other areas is avoided, and the space of the work base can be maximally saved.
In another embodiment of the present invention, a mechanical arm assembly for driving the tray moving claw 3 to perform position adjustment is disposed on a vertical surface of the working platform 1, and the mechanical arm assembly includes an x-axis mechanical arm 103 for driving the tray moving claw 3 to move along an x-axis, a y-axis mechanical arm 104 for driving the tray moving claw 3 to move along a y-axis, and a z-axis mechanical arm 105 for driving the tray moving claw 3 to move along a z-axis.
A method of using a liquid workstation for solid phase extraction and chip array spotting, comprising the steps of:
s1, preparing reagents and consumables (reagent A, reagent B, reagent C, reagent D, reagent E, reagent F, reagent G, reagent H, 12 SPE columns, 12 serum samples to be processed, 50ul and 1000ul gun heads, 12 centrifuge tubes with 2mL, 12 liquid phase vials with 1.5mL and 1 MADLI chip) in advance, and manually placing the reagents to corresponding positions;
the gun head placing module is used for placing gun heads with the specification of 1000ul and 50 ul; 12 2mL centrifuge tubes are placed in the product collection module, and 12 1mLSPE columns are placed in the solid phase extraction module; the sample application module is respectively used for placing 12 liquid phase vials and a MADLI chip;
oscillating the temperature control module 11 to place 12 2mL centrifuge tubes; the sample module 12 is used for placing 12 blood collection tubes containing the serum to be treated;
s2, respectively carrying out reagent injection operation on the liquid injector on the mechanical arm assembly to the waste liquid level of the solid phase extraction module 10 to fill the pipeline with the reagent;
taking 50 mu L of gun head from the gun head placing module by the mechanical arm assembly, taking 30 mu L of supernatant from the blood sampling tube, adding the supernatant into a centrifuge tube on the oscillation temperature control module, and taking off the gun head from the mechanical arm assembly to the gun head waste groove;
the mechanical arm assembly takes 50 mu L of gun head from the gun head placing module to the reagent storage module, 30 mu LA reagent is added into a centrifuge tube in the centrifuge tube adapter, the temperature is raised to 65 ℃, the temperature is reduced to 45 ℃ after 1500rmp is 5min, the mechanical arm assembly takes 50 mu L of gun head from the gun head placing module to the reagent storage module, 4 mu LB reagent is added into the centrifuge tube in the centrifuge tube adapter, and 1500rmp is oscillated for 20min at 45 ℃; the mechanical arm assembly moves the solid-phase extraction rack to the solid-phase extraction module while the oscillation temperature control module oscillates for 20 min; adding 1mLE reagent into SPE column in solid phase extraction rack; the mechanical arm assembly takes a positive pressure head from the positive pressure module to the solid phase extraction column frame to pressurize and pass through the column; the mechanical arm assembly moves the positive pressure head back to the positive pressure module; adding 1mLG reagent to SPE solid phase extraction rack; the mechanical arm assembly takes a positive pressure head from the positive pressure module to the SPE solid phase extraction rack to pressurize and pass through the column; the mechanical arm assembly moves the positive pressure head back to the positive pressure module; adding 1mLE reagent to SPE extraction column rack; the mechanical arm assembly takes a positive pressure head from the positive pressure module to the SPE extraction column frame to pressurize and pass through the column; the mechanical arm assembly moves the positive pressure head back to the positive pressure module; the mechanical arm assembly takes 50 mu L of gun heads from the gun head placing module to the reagent storage module, and takes 5 mu LC of reagent to add into a centrifuge tube adapter centrifuge tube; adding 540 mu LE reagent into the adapter of the centrifuge tube from the reagent spraying device, and adding 100 mu LF reagent into the adapter of the centrifuge tube from the reagent spraying device; the mechanical arm assembly takes 1000 mu L of gun head from the gun head placing module to the centrifuge tube adapter and takes 709 mu L of mixed sample to the SPE extraction column rack for sample loading; the mechanical arm assembly takes a positive pressure head from the positive pressure module to the SPE extraction column frame to pressurize and pass through the column;
the mechanical arm assembly moves the positive pressure head back to the positive pressure module; adding 1mLE reagent from the reagent spraying device 1 to the SPE extraction column rack, taking a positive pressure head from the positive pressure module by the mechanical arm component to the SPE extraction column rack to pressurize and pass through the column, moving the positive pressure head back to the positive pressure module by the mechanical arm component, and repeating the step for 2 times; the mechanical arm assembly stacks the SPE extraction column frame on the product collection frame; adding 1mLG reagent into SPE extraction column rack by reagent injector 3; the mechanical arm assembly takes a positive pressure head from the positive pressure module to the SPE extraction column frame to pressurize and pass through the column; the mechanical arm assembly moves the positive pressure head back to the positive pressure module; the mechanical arm assembly is used for connecting the SPE extraction rack on the product collection adapter to the solid phase extraction column module; moving the nitrogen blowing head above the centrifugal tube adapter by a mechanical claw, blowing nitrogen at 80 ℃, and descending the nitrogen blowing head at regular intervals until the product is dried; the mechanical arm assembly moves the nitrogen blowing head back to the nitrogen blowing module; cooling the temperature control assembly below the collection frame module to 25-37 ℃; adding 15 mu LE reagent into the reagent sprayer and the centrifuge tube adapter; the mechanical arm assembly takes the gun head from the gun head placing module to a centrifuge tube, the gun head is sucked, sprayed and uniformly mixed up and down, and all product liquid is transferred to a liquid phase small bottle; heating a temperature control assembly below the chip adapter to 80 ℃; a mechanical arm assembly cluster gun head placing module takes a gun head to a liquid phase small bottle, and takes 2ul of product microarray to be spotted on the MADLI chip; the liquid phase vial product and the MADLI chip taken out from the sample application module at the end of the experiment can be used for measuring liquid phase and flight mass spectra, analyzing the components of the sample and being used for diagnosis.
The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, it is possible to make several variations and modifications without departing from the concept of the present invention, and these should be considered as the protection scope of the present invention, which will not affect the effect of the implementation of the present invention and the utility of the patent.
Claims (10)
1. The utility model provides a liquid work station for solid phase extraction and chip array spotting, a serial communication port, including work platform (1) of L type, a plurality of work stations that set up on work platform (1) horizontal plane and move a set claw (3) that snatchs work stations, work stations is including malleation module (4), nitrogen blow module (5), rifle head place module (6), result collection module (7), spotting module (8), reagent storage module (9), solid phase extraction module (10), oscillation temperature control module (11) and sample module (12), wherein:
the positive pressure module (4) comprises a positive pressure head main part (41) and a positive pressure head seat (47) and is used for pressurizing the SPE column, the lower part of the positive pressure head main part (41) is fixedly connected with the positive pressure head seat (47), and the upper end and the lower end of the positive pressure head seat (47) are open and hollow; the upper part of the positive pressure head main part (41) is fixedly provided with an elbow joint (44), one end of the elbow joint (44) is communicated with a pipeline, the other end of the elbow joint (44) is communicated with the positive pressure head main part (41), and the pipeline is communicated with an air compressor; a positive pressure sealing ring (45) and a sealing ring fixing plate (46) are arranged between the positive pressure head main part (41) and the positive pressure head seat (47), and a plurality of through holes for gas to flow are formed in the sealing ring fixing plate (46);
the nitrogen blowing module (5) comprises a nitrogen blowing seat (51) and a plurality of nitrogen blowing needles (52) fixedly arranged at the bottom of the nitrogen blowing seat (51), the nitrogen blowing seat (51) is hollow, the nitrogen blowing needles (52) are communicated with the nitrogen blowing seat (51), a sealing cover (55) is arranged at the top of the nitrogen blowing seat (51), and the side edge of the nitrogen blowing seat (51) is communicated with a nitrogen quick connector (54);
a temperature control adapter is arranged below the product collecting module (7), a collecting pipe frame (15) is arranged above the product collecting module, the collecting pipe frame (15) comprises a test tube and a centrifuge tube, and an SPE column and a nitrogen blowing module (5) are respectively stacked on the collecting pipe frame (15);
the collection pipe support (15) comprises an SPE column frame (1501), and an SPE collection pipe (1503) used for storing an SPE column (1502) is arranged in the SPE column frame (1501);
the sample application module (8) comprises a small bottle adapter arranged on the right side and a chip adapter arranged on the right side and used for fixing a chip, and a temperature control adapter is arranged at the bottom of the chip adapter;
the reagent storage module (9) comprises a heat-insulating shell (909) and a semiconductor refrigerating sheet (905) arranged at the bottom of the heat-insulating shell (909), a cold conducting plate (908) is arranged between the heat-insulating shell (909) and the semiconductor refrigerating sheet (905), and an upper refrigerating adapter (907) for storing a plurality of containers is arranged at the upper part of the heat-insulating shell (909);
the oscillation temperature control module (11) comprises an oscillator upper plate (1010), an eccentric shaft (1005) and an oscillation motor (1001), a host connecting plate (1007) is fixedly installed below the oscillator upper plate (1010), the oscillation motor (1001) is fixedly installed at the bottom of the host connecting plate (1007) through an oscillation motor base (1002), and the output end of the oscillation motor (1001) is fixedly connected with the eccentric shaft (1005).
2. The liquid workstation for solid phase extraction and chip array spotting of claim 1 wherein two pin fixing plates I are symmetrically installed on the left and right sides of the upper part of the positive indenter main part (41), and a plurality of pins I (42) for fixing the positive indenter main part (41) are fixedly installed on the pin fixing plates I.
3. The liquid workstation for solid phase extraction and chip array spotting according to claim 1 characterized in that the nitrogen fast joint (54) communicates with a nitrogen gas cylinder and the nitrogen blow pins (52) are provided in 12 number.
4. The liquid workstation for solid phase extraction and chip array spotting according to claim 3 characterized in that the nitrogen blowing sealing ring (53) is arranged at the connection of the nitrogen blowing seat (51) and the sealing cover (55).
5. The liquid workstation for solid phase extraction and chip array spotting of claim 4, wherein a fan bracket (902) is arranged below the semiconductor refrigeration piece (905), a fan (901) is installed on the fan bracket (902), and a copper radiator (903) and a heat conducting plate (904) are further arranged between the semiconductor refrigeration piece (905) and the fan (901).
6. The liquid workstation for solid phase extraction and chip array spotting according to claim 1 characterized in that the upper plate (1010) of the oscillator is connected with a collection tube adapter (1012), several collection tubes (1014) driven by the eccentric shaft (1005) are placed on the collection tube adapter (1012); the collecting pipe adapter (1012) is connected with the upper plate (1010) of the oscillator through a pin fixing plate II (1011).
7. The liquid workstation for solid phase extraction and chip array spotting according to claim 6 characterized in that the connection of the output end of the oscillating motor (1001) and the eccentric shaft (1005) is further provided with a balance weight (1006) and a return spring (1004) fitting with the eccentric shaft (1005).
8. The liquid workstation for solid phase extraction and chip array spotting of claim 1 wherein the sample module (12) comprises a lower sample holder plate (1201) and an upper sample holder plate (1203), the lower sample holder plate (1201) and the upper sample holder plate (1203) are connected and fixed by a support column (1202).
9. The liquid workstation for solid phase extraction and chip array spotting of claim 1 wherein the positive pressure module (4), the nitrogen blowing module (5), the gun head placing module (6), the product collecting module (7), the spotting module (8), the reagent storage module (9), the solid phase extraction module (10), the oscillation temperature control module (11) and the sample module (12) are arranged in a grid manner.
10. The liquid working station for solid phase extraction and chip array spotting according to any of claims 1-9 characterized in that a robotic arm assembly for driving the tray moving claw (3) to adjust the position is disposed on the vertical surface of the working platform (1), and the robotic arm assembly comprises an x-axis robotic arm (103) for driving the tray moving claw (3) to move along the x-axis, a y-axis robotic arm (104) for driving the tray moving claw (3) to move along the y-axis, and a z-axis robotic arm (105) for driving the tray moving claw (3) to move along the z-axis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120299322.6U CN215375258U (en) | 2021-02-02 | 2021-02-02 | Liquid workstation for solid phase extraction and chip array sample application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120299322.6U CN215375258U (en) | 2021-02-02 | 2021-02-02 | Liquid workstation for solid phase extraction and chip array sample application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215375258U true CN215375258U (en) | 2021-12-31 |
Family
ID=79622232
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120299322.6U Active CN215375258U (en) | 2021-02-02 | 2021-02-02 | Liquid workstation for solid phase extraction and chip array sample application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215375258U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022166766A1 (en) * | 2021-02-02 | 2022-08-11 | 药明激创(佛山)生物科技有限公司 | Liquid workstation for solid-phase extraction and chip array sample application |
| CN115032413A (en) * | 2022-08-15 | 2022-09-09 | 杭州凯莱谱质造科技有限公司 | Sampling device for sample pretreatment and pretreatment method |
-
2021
- 2021-02-02 CN CN202120299322.6U patent/CN215375258U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022166766A1 (en) * | 2021-02-02 | 2022-08-11 | 药明激创(佛山)生物科技有限公司 | Liquid workstation for solid-phase extraction and chip array sample application |
| CN115032413A (en) * | 2022-08-15 | 2022-09-09 | 杭州凯莱谱质造科技有限公司 | Sampling device for sample pretreatment and pretreatment method |
| CN115032413B (en) * | 2022-08-15 | 2023-01-03 | 杭州凯莱谱质造科技有限公司 | Sampling device for sample pretreatment and pretreatment method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN215375258U (en) | Liquid workstation for solid phase extraction and chip array sample application | |
| US11524287B2 (en) | Automatic pipetting device for transferring samples and/or reagents and method for transferring liquid samples and/or reagents | |
| CN107904156B (en) | Integrated full-automatic digital PCR detection system and implementation method | |
| CN112858540A (en) | Liquid workstation for solid phase extraction and chip array sample application | |
| US6964867B2 (en) | Method and apparatus for performing multiple processing steps on a sample in a single vessel | |
| CN206096158U (en) | Automatic liquid processing system | |
| US20080254545A1 (en) | Apparatus and method for biochemical analysis | |
| JP2003505711A (en) | Low volume chemical and biochemical reaction systems | |
| US20030091473A1 (en) | Automated centrifuge and method of using same | |
| CN106701527A (en) | Reagent workstation | |
| CN102533525A (en) | Full-automatic hybridization appliance | |
| US20230366790A1 (en) | Full-automatic preparation method of cast-off cell smear | |
| CN107045069A (en) | A kind of specific protein analyzer reaction cup handling system and its application method | |
| CN111979119A (en) | Full-automatic cell passage operation device and method | |
| CN115494803A (en) | Full-flow integrated medicine research and development workstation | |
| CN201942671U (en) | Fully automatic working station for polymerase chain reaction amplification | |
| CN201524578U (en) | Reaction tray | |
| CN112210476A (en) | Be applied to biological liquid workstation | |
| CN116676183A (en) | Molecular diagnosis system and molecular diagnosis method | |
| CN216747039U (en) | Full-automatic sample pretreatment device | |
| CN202430210U (en) | Full-automatic hybridization instrument | |
| CN214830378U (en) | Biological experiment operating equipment | |
| CN115201476A (en) | Circulating tumor cell detection equipment and use method thereof | |
| CN212247057U (en) | Full-automatic cell passage operation device | |
| CN209513393U (en) | Integrate heating, nitrogen is blown and the 96 orifice plate processing instruments that shake |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |