CN215327840U - Device for purifying multidimensional protein - Google Patents
Device for purifying multidimensional protein Download PDFInfo
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- CN215327840U CN215327840U CN202121878791.XU CN202121878791U CN215327840U CN 215327840 U CN215327840 U CN 215327840U CN 202121878791 U CN202121878791 U CN 202121878791U CN 215327840 U CN215327840 U CN 215327840U
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- dimensional protein
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Abstract
The utility model discloses a device for multi-dimensional protein purification, which comprises a base, a support column, a chromatographic column fixing support and a tray, wherein the support column is arranged on the base; the bottom end of the supporting column is fixed on the base, and the top end of the supporting column is fixedly connected with the bottom end of the tray; the chromatographic column fixing bracket is sleeved on the supporting column and can be fixedly connected with the supporting column; the number of the chromatographic column fixing supports is at least two. The protein purification device is suitable for the purification of multi-dimensional protein, in particular to the protein which needs to be purified by three chromatographic modes of gel chromatography, ion exchange chromatography and hydrophobic chromatography. It can be according to first carrying out gel column chromatography, again through ion column chromatography, last through hydrophobic chromatography column chromatography, needs the protein of this order purification, can prepare and handle three chromatographic column simultaneously, has improved the efficiency of operation greatly, and it is convenient to install fixed chromatographic column.
Description
Technical Field
The utility model relates to the technical field of biological purification, in particular to a device for purifying multi-dimensional protein.
Background
Proteins, which are the basic components of living bodies and the ultimate controllers and executives of life activities, are involved in almost all life activities, and human studies on proteins have a history of over a hundred years. The protein analysis and purification equipment is an important instrument for researching the structure and the composition of the protein and is also core equipment for developing protein medicaments. Common methods of protein isolation include: affinity chromatography, gel filtration chromatography, ion exchange chromatography, hydrophobic chromatography, and the like. The purification of the protein may involve a combination of the above-mentioned methods.
In the process of applying the chromatography method, the chromatography column and the chromatography medium used in the chromatography column are often treated, and the chromatography column needs to be disassembled; and in the whole process of chromatography, a protein sample purified by one chromatographic column needs to be processed by another chromatographic column, so that a device required for multi-dimensional protein purification is urgently needed in the process of multi-dimensional protein purification.
Disclosure of Invention
To this end, the present invention provides an apparatus for multi-dimensional protein purification.
In order to achieve the above purpose, the utility model provides the following technical scheme:
the utility model provides a device for multi-dimensional protein purification, which comprises a base, a support column, a chromatographic column fixing support and a tray, wherein the support column is arranged on the base;
the bottom end of the supporting column is fixed on the base, and the top end of the supporting column is fixedly connected with the bottom end of the tray;
the chromatographic column fixing support is sleeved on the support column and can be fixedly connected with the support column.
The number of the chromatographic column fixing supports is at least two.
In one embodiment of the utility model, the fixing bracket comprises a sleeve, a support rod fixed with the sleeve, and a clamping clamp fixed at the tail end of the support rod, wherein the sleeve is provided with a screw hole for fixing a locking bolt.
In one embodiment of the utility model, the number of the holding clips is 3.
In one embodiment of the utility model, a sample feeding peristaltic pump is mounted on the tray.
In an embodiment of the present invention, the tray is further provided with a receiving groove for receiving the sampling cup.
In one embodiment of the utility model, the apparatus further comprises a cabinet body comprising a lower base plate and an upper base plate, the upper base plate being capable of carrying the ultraviolet detector, the lower base plate carrying the component collector.
In an embodiment of the utility model, a sleeve sleeved with the support column is arranged at the bottom of the tray, and a bolt for fixing the sleeve is arranged on the sleeve.
In one embodiment of the present invention, the device further comprises a gel chromatography column, an ion exchange column and a hydrophobic chromatography column respectively fixed on the chromatography column fixing support.
The utility model has the following advantages:
the protein purification device is suitable for the purification of multi-dimensional protein, in particular to the protein which needs to be purified by three chromatographic modes of gel chromatography, ion exchange chromatography and hydrophobic chromatography. It can be according to first carrying out gel column chromatography, again through ion column chromatography, last through hydrophobic chromatography column chromatography, needs the protein of this order purification, can prepare and handle three chromatographic column simultaneously, has improved the efficiency of operation greatly, and it is convenient to install fixed chromatographic column.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions that the present invention can be implemented, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the effects and the achievable by the present invention, should still fall within the range that the technical contents disclosed in the present invention can cover.
Fig. 1 is a schematic structural diagram of an apparatus for multi-dimensional protein purification according to an embodiment of the present invention;
FIG. 2 is a schematic structural diagram of an apparatus for multi-dimensional protein purification according to another embodiment of the present invention;
FIG. 3 is a schematic structural diagram of a chromatographic column fixing bracket provided in an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a bottom of a tray according to an embodiment of the present invention;
in the figure: 100-a base; 200-support column; 300-chromatographic column fixing support; 310-a strut; 320-a clamping clip; 330-a sleeve; 331-locking bolt; 332-a support bar; 400-a tray; 500-cabinet body; 510-an upper base plate; 520-lower floor.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the utility model will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the utility model and that it is not intended to limit the utility model to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1, 3 and 4, the present invention provides an apparatus for multi-dimensional protein purification, the protein purification apparatus including a base 100, a support column 200, a chromatography column fixing bracket 300 and a tray 400; the bottom end of the supporting column 200 is fixed on the base 100, and the top end of the supporting column 200 is fixedly connected with the bottom end of the tray 400; the chromatographic column fixing bracket 300 is sleeved on the support column 200 and can be fixedly connected with the support column 200.
Wherein, the quantity of chromatography column fixed bolster 300 is two at least, and the fixed bolster includes sleeve pipe 330, with sleeve pipe 330 fixed branch 310 to and be fixed in the terminal centre gripping of branch 310 and press from both sides 320, be equipped with the screw that supplies locking bolt 331 to fix on sleeve pipe 330, can be fixed in support column 200 with sleeve pipe 330 through fixing bolt, be about to be fixed in on support column 200 chromatography column fixed bolster 300, chromatography column fixed bolster 300 is from last down being fixed in support column 200 in proper order.
As an alternative embodiment, as shown in FIG. 2, when there are two chromatographic column fixing supports 300, a support rod 332 is disposed on the lower fixing support, one end of the support rod 332 is connected to the bottom end of the chromatographic column fixing support, and the other end is connected to the base 100, the support rod 332 can ensure the horizontal and fixed position of the chromatographic column fixing support 300
Preferably, the number of the clamping clips 320 is 3, the clamping clips 320 are uniformly distributed on the fixing support along the circumferential direction, three chromatography columns can be fixed by the three clamping clips 320, and the sleeve 330 can rotate along the support column 200 as required, so that the chromatography columns in required operation are fixed in a certain direction convenient for operation. The fixing bracket of the utility model is provided with three clamping clamps 320, which can be used for fixing gel chromatography columns, ion exchange columns and hydrophobic chromatography columns. Thus, it is suitable for proteins that are purified by gel chromatography, then ion exchange, and finally hydrophobic chromatography in sequence.
The tray 400 of the device for multi-dimensional protein purification is provided with sample feeding peristaltic pumps, and the number of the peristaltic pumps which can be accommodated is 3. Still be equipped with the storage tank that can hold the sample cup on the tray 400, the quantity of storage tank is 3, can operate three chromatography column simultaneously, also can be according to the purification order of protein, uses the chromatography column several times, has improved the convenience of operation greatly, has improved the availability factor of chromatography column.
In addition, the bottom of the tray 400 is provided with a sleeve 330 which is sleeved with the support column 200, the sleeve 330 is provided with a bolt for fixing the sleeve 330, when the tray 400 is fixed, the sleeve 330 of the tray 400 is sleeved on the support column 200, and then the bolt is tightened to fix the tray 400 on the top end of the support column 200.
The apparatus of the present invention further comprises a cabinet 500, the cabinet 500 comprising a lower plate 520 and an upper plate 510, the upper plate 510 carrying the uv detector and the lower plate 520 carrying the component collector. The lower plate 520 of the cabinet 500 is connected to the base 100 and located at one side of the base 100, so as to facilitate the detection and collection of purified protein.
According to the device for multidimensional protein purification, the chromatographic columns are treated at the same time, the protein can be purified by firstly carrying out gel column chromatography, then carrying out ion column chromatography and finally carrying out hydrophobic chromatography, the sequentially purified protein is required, the three chromatographic columns can be prepared and treated at the same time, the operation efficiency is greatly improved, and the chromatographic columns are convenient to install and fix.
Although the utility model has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the utility model. Accordingly, such modifications and improvements are intended to be within the scope of the utility model as claimed.
Claims (8)
1. An apparatus for multi-dimensional protein purification, wherein the protein purification apparatus comprises a base, a support column, a chromatography column fixing support and a tray;
the bottom end of the supporting column is fixed on the base, and the top end of the supporting column is fixedly connected with the bottom end of the tray;
the chromatographic column fixing bracket is sleeved on the supporting column and can be fixedly connected with the supporting column;
the number of the chromatographic column fixing supports is at least two.
2. The apparatus for multi-dimensional protein purification of claim 1,
the fixing support comprises a sleeve, a supporting rod fixed with the sleeve and a clamping clamp fixed at the tail end of the supporting rod, and a screw hole for fixing a locking bolt is formed in the sleeve.
3. The apparatus for multi-dimensional protein purification of claim 2,
the number of the clamping clips is 3.
4. The apparatus for multi-dimensional protein purification of claim 1,
and a sample injection peristaltic pump is arranged on the tray.
5. The apparatus for multi-dimensional protein purification of claim 1,
the tray is also provided with a containing groove capable of containing the sampling cup.
6. The apparatus for multi-dimensional protein purification of claim 1,
the device also comprises a cabinet body, wherein the cabinet body comprises a lower bottom plate and an upper bottom plate, the upper bottom plate can bear the ultraviolet detector, and the lower bottom plate bears the component collector.
7. The apparatus for multi-dimensional protein purification of claim 1,
the tray bottom be equipped with the sleeve pipe that the support column cover was established, be equipped with on the sleeve pipe and be used for fixing sheathed tube bolt.
8. The apparatus for multi-dimensional protein purification of claim 1,
the device also comprises a gel chromatographic column, an ion exchange column and a hydrophobic chromatographic column which are respectively fixed on the chromatographic column fixing support.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121878791.XU CN215327840U (en) | 2021-08-11 | 2021-08-11 | Device for purifying multidimensional protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121878791.XU CN215327840U (en) | 2021-08-11 | 2021-08-11 | Device for purifying multidimensional protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215327840U true CN215327840U (en) | 2021-12-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121878791.XU Active CN215327840U (en) | 2021-08-11 | 2021-08-11 | Device for purifying multidimensional protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215327840U (en) |
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2021
- 2021-08-11 CN CN202121878791.XU patent/CN215327840U/en active Active
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