CN215250916U - Cell culture dish device convenient to organize piece equidistance adherence - Google Patents

Cell culture dish device convenient to organize piece equidistance adherence Download PDF

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Publication number
CN215250916U
CN215250916U CN202120586892.3U CN202120586892U CN215250916U CN 215250916 U CN215250916 U CN 215250916U CN 202120586892 U CN202120586892 U CN 202120586892U CN 215250916 U CN215250916 U CN 215250916U
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China
Prior art keywords
culture dish
adherence
cell culture
placing part
cover body
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CN202120586892.3U
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Chinese (zh)
Inventor
段安琴
邓廷贤
陆杏蓉
于童
马小娅
梁莎莎
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GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INSTITUTE
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GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INSTITUTE
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Abstract

The utility model discloses a cell culture dish device convenient to organize piece equidistance adherence belongs to the laboratory with the apparatus field. The culture dish comprises a culture dish body and a cover body, wherein the culture dish body is made of transparent or semitransparent materials, the cover body covers the opening of the culture dish body, a placing part is arranged on the upper end surface of the cover body, and a plurality of positioning mark points are arranged on the placing part; the upper end surface of the placing part is matched with the lower end surface of the culture dish body in a fitting manner. The technical problems that when the tissue blocks are attached to the wall for separating and culturing cells, the arrangement intervals of the tissue blocks are different, so that the migration of the cells is blocked or culture dishes and culture solution are wasted are solved.

Description

Cell culture dish device convenient to organize piece equidistance adherence
Technical Field
The utility model relates to an apparatus field is used in the laboratory, especially relates to a cell culture dish device convenient to organize piece equidistance adherence.
Background
Cell culture (cell culture) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the cells to survive, grow and reproduce and maintain main structures and functions. Tissue mass culture is one of the important methods for cell culture; when the tissue block adheres to the wall and separates and cultures cells, the tissue block needs to be cut to 1mm3Size, tissue blocks were attached to petri dishes at 1cm intervals. Most people for 1mm3The sizes have no specific mapping, and the size difference of the organization blocks is large. When the tissue blocks are attached to the wall, the tissue blocks can be attached only by feel, and when the tissue blocks are arranged too densely, the migration of cells can be blocked; when the tissue blocks are arranged too sparsely, the waste of culture dishes and culture solution is caused.
Disclosure of Invention
The utility model aims at providing a cell culture dish device convenient to tissue piece equidistance adherence to wall to above-mentioned problem, when solving tissue piece adherence separation culture cell, tissue piece array interval is not of uniform size, causes the cell to emigrating and can be obstructed or the technical problem of culture dish, culture solution waste.
In order to achieve the above purpose, the utility model adopts the technical proposal that:
a cell culture dish device convenient for tissue blocks to adhere to the wall at equal intervals comprises a culture dish body and a cover body, wherein the culture dish body is made of transparent or semitransparent materials, the cover body covers an opening of the culture dish body, a placing part is arranged on the upper end surface of the cover body, and a plurality of positioning mark points are arranged on the placing part; the upper end surface of the placing part is matched with the lower end surface of the culture dish body in a fitting manner.
Furthermore, a plurality of positioning mark points are uniformly distributed on the upper end surface of the placing part.
Furthermore, the distance between each positioning mark point is 1 cm.
Furthermore, a plurality of clamping points are arranged on the lower end face of the culture dish body and the surface of the cover body which is placed to be abutted.
Furthermore, a plurality of clamping grooves are formed in the outer edge of the placing portion, a plurality of clamping convex strips are arranged on the lower end face of the culture dish body, and the clamping grooves correspond to the clamping convex strips one to one and are clamped with the clamping convex strips.
Furthermore, the number of the clamping grooves is three or six, and the clamping grooves are uniformly distributed around the center of the cover body.
Further, the positioning mark points are squares of 1mm by 1 mm.
Due to the adoption of the technical scheme, the beneficial effects of the utility model are that:
1. when the utility model is used for adherent separation culture of cells by tissue blocks, the culture dish body is placed on the monomer placing part, and the position of the mark point can be directly seen through the culture dish body because the culture dish body is made of transparent or semitransparent materials; directly place the tissue piece that will cut into the piece in the top that corresponds the alignment mark point, can realize arranging the tissue piece according to setting for the position interval, when solving tissue piece adherent isolation and culturing cell, tissue piece arrangement interval is not of uniform size, causes the cell to emigrating or culture dish, the extravagant technical problem of culture solution.
2. The utility model discloses a be provided with the snap-on point, prevent to touch the culture dish body carelessly placing the in-process of arranging the tissue piece, make the culture dish body and take the lid of mark point to take place relative displacement to cause the off tracking of alignment mark point, cause the change of influence tissue piece interval. The clamping effect is realized in a mode of adopting the clamping grooves and the clamping convex strips, and the clamping structure is simple and convenient.
3. The utility model discloses a with the square of setting to 1mm of mark point, the operator of being convenient for prevents too big or undersize of tissue piece with tissue piece size and mark point contrast, reduces because of the big technical problem that causes the cell emigration to block or culture dish, culture solution are extravagant of tissue piece size difference, improves cell culture's reliability.
Drawings
FIG. 1 is a three-dimensional structure diagram of the present invention;
FIG. 2 is a three-dimensional perspective view of the tissue culture device in a state of being attached to the wall;
in the attached figure, 1-culture dish body, 2-cover body, 11-clamping convex strip, 21-placing part, 22-positioning mark point and 23-clamping groove.
Detailed Description
The following describes the embodiments of the present invention with reference to the accompanying drawings.
In the description of the present invention, it is to be understood that the terms "center", "length", "width", "up", "down", "vertical", "horizontal", "top", "bottom", "inner", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
Referring to fig. 1 and 2, a cell culture dish device for facilitating tissue blocks to adhere to the wall at equal intervals comprises a culture dish body 1 and a cover body 2, wherein the culture dish body 1 is made of transparent or semitransparent materials, the cover body 2 covers an opening of the culture dish body 1, a placing part 21 is arranged on the upper end surface of the cover body 2, and a plurality of positioning mark points 22 are arranged on the placing part 21; the upper end surface of the placing part 21 is matched with the lower end surface of the culture dish body 1 in a fitting manner. Specifically, the culture dish body 1 can be made of glass or plastic. The cover body 2 can also be made transparent or semitransparent, and the positioning mark points 22 are black points.
In this embodiment, the plurality of positioning mark points 22 are uniformly distributed on the upper end surface of the placing portion 21. The distance between the positioning mark points 22 is 1 cm. The lower end surface of the culture dish body 1 and the placing and jointing surface of the cover body 2 are provided with a plurality of clamping points. The outer edge of the placing part 21 is provided with a plurality of clamping grooves 23, the lower end face of the culture dish body 1 is provided with a plurality of clamping convex strips 11, and the clamping grooves 23 and the clamping convex strips 11 are in one-to-one correspondence and are clamped with each other. The number of the clamping grooves 23 is three or six, and the clamping grooves 23 are uniformly distributed around the center of the cover body 2. Through being provided with the snap-on point, prevent to touch culture dish body 1 at the in-process of placing the range tissue piece carelessly, make culture dish body 1 and take place relative displacement with lid 2 of mark point to cause the off tracking of positioning mark point 22, cause and influence tissue piece interval change. The clamping effect is realized by adopting the clamping groove 23 and the clamping convex strip 11, and the clamping structure is simple and convenient.
In this embodiment, the positioning mark points 22 are squares of 1mm by 1 mm. Through setting the square of 1mm with the setting of mark point, the operator of being convenient for compares tissue piece size and mark point, prevents too big or undersize of tissue piece, reduces because of the big technical problem that causes the cell migration can be obstructed or culture dish, culture solution are extravagant of tissue piece size difference, improves cell culture's reliability.
When the culture dish is used for adherent isolation culture of cells by tissue blocks, the culture dish body 1 is placed on the monomer placing part 21, and the positions of the mark points can be directly seen through the culture dish body 1 because the culture dish body 1 is made of transparent or semitransparent materials; directly place the tissue piece that will cut into the piece in the top that corresponds alignment mark point 22, can realize arranging the tissue piece according to setting for the position interval, when solving tissue piece adherent isolation cell culture, tissue piece arrangement interval is not of uniform size, causes the cell to emigrating and can be hindered or culture dish, the extravagant technical problem of culture solution.
The above description is for the detailed description of the preferred possible embodiments of the present invention, but the embodiments are not intended to limit the scope of the present invention, and all equivalent changes or modifications accomplished under the technical spirit suggested by the present invention should fall within the scope of the present invention.

Claims (7)

1. The utility model provides a cell culture dish device convenient to piece equidistance adherence which characterized in that: the culture dish comprises a culture dish body and a cover body, wherein the culture dish body is made of transparent or semitransparent materials, the cover body covers the opening of the culture dish body, a placing part is arranged on the upper end surface of the cover body, and a plurality of positioning mark points are arranged on the placing part; the upper end surface of the placing part is matched with the lower end surface of the culture dish body in a fitting manner.
2. A cell culture dish device for facilitating equidistant adherence of tissue blocks according to claim 1, wherein: and the positioning mark points are uniformly distributed on the upper end surface of the placing part.
3. A cell culture dish device for facilitating equidistant adherence of tissue blocks according to claim 2, wherein: the distance between each positioning mark point is 1 cm.
4. A cell culture dish device for facilitating equidistant adherence of tissue blocks according to claim 1, wherein: the lower end face of the culture dish body and the face where the cover body is placed to be abutted are provided with a plurality of clamping points.
5. A cell culture dish device for facilitating equidistant adherence of tissue blocks according to claim 4, wherein: the outer edge of the placing part is provided with a plurality of clamping grooves, the lower end face of the culture dish body is provided with a plurality of clamping convex strips, and the clamping grooves correspond to the clamping convex strips one to one and are clamped with the clamping convex strips.
6. A cell culture dish device for facilitating equidistant adherence of tissue blocks according to claim 5, wherein: the number of the clamping grooves is three or six, and the clamping grooves are uniformly distributed around the center of the cover body.
7. A cell culture dish device for facilitating equidistant adherence of tissue blocks according to claim 1, wherein: the positioning mark points are squares of 1mm by 1 mm.
CN202120586892.3U 2021-03-23 2021-03-23 Cell culture dish device convenient to organize piece equidistance adherence Active CN215250916U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202120586892.3U CN215250916U (en) 2021-03-23 2021-03-23 Cell culture dish device convenient to organize piece equidistance adherence

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202120586892.3U CN215250916U (en) 2021-03-23 2021-03-23 Cell culture dish device convenient to organize piece equidistance adherence

Publications (1)

Publication Number Publication Date
CN215250916U true CN215250916U (en) 2021-12-21

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CN202120586892.3U Active CN215250916U (en) 2021-03-23 2021-03-23 Cell culture dish device convenient to organize piece equidistance adherence

Country Status (1)

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CN (1) CN215250916U (en)

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