CN215209437U

CN215209437U - Reagent strip, bearing support and deep hole board

Info

Publication number: CN215209437U
Application number: CN202120785492.5U
Authority: CN
Inventors: 彭彪; 赵一帆; 解亚平; 戴立忠; 范旭
Original assignee: Sansure Biotech Inc
Current assignee: Sansure Biotech Inc
Priority date: 2021-04-16
Filing date: 2021-04-16
Publication date: 2021-12-17
Anticipated expiration: 2031-04-16

Abstract

The utility model relates to a reagent strip and be equipped with its deep hole board, deep hole board includes: the bearing bracket is provided with an accommodating space with one open end; at least one reagent strip, each reagent strip comprising a plurality of reagent tubes, each reagent tube having an oppositely disposed closed end and an open end; the closed end of all the reagent tubes of each reagent strip is inserted into the accommodating space from the open end of the accommodating space. Above-mentioned deep hole board, can keep with the appearance of the deep hole board among the prior art under the unanimous prerequisite of appearance, through the nimble setting to the quantity of reagent strip, can realize whole board nucleic acid extraction and can realize the nucleic acid extraction of small dimension again, consequently not only can satisfy the extraction requirement to small sample volume, also can realize the assembly line of large sample volume and draw, avoided the waste of small batch volume, scattered sample detection simultaneously, practiced thrift the detection cost.

Description

Reagent strip, bearing support and deep hole board

Technical Field

The utility model relates to an external diagnosis technical field especially relates to a reagent strip, bears support and deep hole board.

Background

The deep hole board is on the basis of the apparent dimension of ordinary micropore board, when keeping length, width accord with SBS international standard, increase the degree of depth in hole, thereby reach the laboratory panel of the volumetric purpose that increases each hole, wide application in fields such as biological research, detection, both can replace conventional centrifuging tube and store the sample, can also combine the calandria, high flux automatic liquid operation instrument and software, realize the high flux operation to the biological sample, also can be generally used in various automatic sample injectors, can directly put the sample introduction in the sample storehouse of automatic sample injector, put clean and tidy when having the storage, save space, the storage capacity is big, characteristics such as treatment effeciency height.

In particular, in nucleic acid detection, the deep-well plate can be applied to nucleic acid extraction and purification on a nucleic acid extraction instrument and used as a disposable consumable. Specifically, nucleic acid purification is achieved by loading and lysing in a deep well plate, and then further performing an amplification Reaction in a PCR (Polymerase Chain Reaction) instrument, which can specifically amplify a very minute amount of target DNA by millions of times, thereby facilitating analysis and detection of DNA molecules.

However, the current deep-well plate can only be used once for processing a large number of samples, and when only a small number of samples and scattered samples need to be processed, only a small part of the area of the deep-well plate is actually used, but the whole deep-well plate is discarded after the small part of the area is used, so that the waste of reagents and the consumables of the deep-well plate is caused, and the detection cost is increased.

SUMMERY OF THE UTILITY MODEL

Therefore, there is a need for a reagent strip, a carrying bracket and a deep well plate, which can achieve the technical effect of being compatible with small-batch sample experiments on the basis of realizing large-batch sample experiments.

According to an aspect of the present application, there is provided a deep hole plate including:

the bearing bracket is provided with an accommodating space with one open end; and

at least one reagent strip, each of said reagent strips comprising a plurality of reagent tubes, each of said reagent tubes having oppositely disposed closed and open ends;

wherein the closed end of all the reagent tubes of each reagent strip is inserted into the accommodating space from the open end of the accommodating space.

In one embodiment, a plurality of the reagent strips are arranged at intervals along a first direction, all the reagent tubes in each reagent strip are sequentially connected along a second direction, and each reagent tube is inserted into the accommodating space along a third direction;

wherein the first direction, the second direction and the third direction are perpendicular to each other.

In one embodiment, each reagent strip further includes two limiting members, the two limiting members are located at two opposite ends of the reagent strip in the second direction, and the two limiting members are respectively limited at two side edges of the open end of the carrying bracket in the second direction.

In one embodiment, the position-limiting member is supported by an edge of an open end of the bearing bracket in the third direction.

In one embodiment, the edge of each of two opposite sides of the bearing bracket in the second direction is provided with a fixing groove having a bottom wall, and the limiting member is limited in the fixing groove.

In one embodiment, the limiting member can be deformed in the second direction in a recoverable manner, a limiting slot is formed in a bottom wall of the fixing slot, and the limiting member is clamped in the limiting slot.

In one embodiment, the cavity walls of the two opposite sides of the accommodating cavity in the second direction are respectively provided with limiting ribs in a protruding manner, a plurality of limiting ribs on the cavity wall of each side are arranged at intervals in the first direction, each limiting rib extends lengthwise along the third direction, and two sides of any one reagent strip in the second direction are respectively limited between two adjacent limiting ribs.

In one embodiment, each reagent strip comprises eight reagent tubes, and the deep-well plate comprises six reagent strips which are arranged at intervals along a straight line direction.

According to one aspect of the present application, there is provided a reagent strip comprising a plurality of reagent tubes, each of the reagent tubes having a closed end and an open end disposed opposite to each other.

In one embodiment, the reagent tubes are sequentially arranged along a straight line, and the reagent strip further includes two limiting members located at two opposite ends of the reagent strip in the arrangement direction of the reagent tubes.

In one embodiment, the cross section of the open end of the reagent tube is rectangular, the end of the closed end of the reagent tube far away from the open end is conical, and the diameter of the end of the closed end of the reagent tube far away from the open end is gradually reduced in the direction from the open end to the closed end.

According to one aspect of the application, a bearing support is provided for accommodating reagent strips, and is characterized in that the bearing support is provided with an accommodating space with one open end, and the reagent strips can be inserted into the accommodating space from the open end of the accommodating space.

In one embodiment, the supporting bracket includes a bracket bottom wall and a bracket side wall, the bracket side wall extends from the edge of the bracket bottom wall toward the same direction, the bracket side wall circumferentially surrounds the bracket bottom wall to form the accommodating space, and a fixing groove having a bottom wall is formed in the edge of one end of the bracket side wall away from the bracket bottom wall.

In one embodiment, the bottom wall of the fixing groove is provided with a limiting clamping groove.

In one embodiment, a plurality of limiting ribs are arranged on the cavity wall of the side wall of the support in a protruding manner, the plurality of limiting ribs are arranged at intervals in a direction perpendicular to the inserting direction of the reagent strips, and the extending direction of each limiting rib is parallel to the inserting direction of the reagent strips.

Above-mentioned deep hole board, can keep with the appearance of the deep hole board among the prior art under the unanimous prerequisite of appearance, through the nimble setting to the quantity of reagent strip, can realize whole board nucleic acid extraction and can realize the nucleic acid extraction of small dimension again, consequently not only can satisfy the extraction requirement to small sample volume, also can realize the assembly line of large sample volume and draw, avoided the waste of small batch volume, scattered sample detection simultaneously, practiced thrift the detection cost.

Drawings

Fig. 1 is a schematic structural diagram of a deep hole plate according to an embodiment of the present invention;

FIG. 2 is a schematic view of a carrier support of the deep hole plate of FIG. 1;

FIG. 3 is a schematic diagram of the structure of the reagent strip of the deep-well plate shown in FIG. 1;

fig. 4 is a schematic structural view of a deep hole plate according to another embodiment of the present invention;

FIG. 5 is a schematic view of the construction of the carrier support of the deep hole plate of FIG. 4;

FIG. 6 is a schematic diagram of the structure of the reagent strip of the deep-well plate shown in FIG. 4.

The reference numbers illustrate:

100. a deep hole plate; 20. a load bearing support; 21. a bottom wall of the bracket; 23. a bracket side wall; 232. fixing grooves; 2321. a limiting clamping groove; 234. limiting ribs; 25. an accommodating chamber; 40. a reagent strip; 41. a reagent tube; 43. a limiting member; 432. a first limiting part; 434. a second limiting part.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Fig. 1 shows a schematic structural diagram of a deep hole plate in an embodiment of the present invention; fig. 2 shows a schematic structural diagram of a carrier of a deep hole plate according to an embodiment of the present invention; fig. 3 shows a schematic structural diagram of a reagent strip of a deep-well plate in an embodiment of the present invention.

Referring to fig. 1 to 3, an embodiment of the present invention provides a deep-well plate 100 for storing and processing samples, and particularly, in the following embodiments, the deep-well plate 100 is applied to a nucleic acid extraction apparatus, and nucleic acid purification is realized by sample loading and cracking in the deep-well plate 100, so as to perform an amplification reaction on a nucleic acid amplification apparatus. It is understood that in other embodiments, the deep well plate 100 may be used in other instruments for different kinds of experiments or tests, and is not limited herein.

As described in the background art, the current deep-well plate can only be used as a whole plate, and the specifications of the deep-well plate are generally 48-well deep-well plate and 96-well deep-well plate, and 48 and 96 samples can be simultaneously processed in batches, so that waste of reagents and deep-well plate consumables is caused when single detection is performed, and the detection cost is increased.

Based on the above problem, the deep well plate 100 of the present application includes a support frame 20 and at least one reagent strip 40, the support frame 20 has an accommodating space with an open end, each reagent strip 40 includes a plurality of reagent tubes 41, each reagent tube 41 has a closed end and an open end which are oppositely disposed, and the closed ends of all the reagent tubes 41 of each reagent strip 40 are inserted into the accommodating space from the open end of the accommodating space.

Above-mentioned deep hole board 100, can keep with the appearance of the deep hole board 100 among the prior art under the unanimous prerequisite of appearance, through the nimble setting to the quantity of reagent strip 40, can realize whole board nucleic acid extraction and can realize the nucleic acid extraction of small dimension again, consequently not only can satisfy the extraction requirement to small sample volume, also can realize the assembly line of large sample volume and draw, has avoided the waste of small batch, scattered sample detection simultaneously, has practiced thrift the detection cost.

Referring to fig. 1 to fig. 3, the supporting frame 20 is a cubic hollow shell structure, and includes a frame bottom wall 21 and a frame side wall 23. The support bottom wall 21 is substantially rectangular, the length direction of the support bottom wall 21 is a first direction, the width direction of the support bottom wall 21 is a second direction, and the thickness direction of the support bottom wall 21 is a third direction, wherein the first direction, the second direction and the third direction are perpendicular to each other. The holder side wall 23 extends from the edge of the holder bottom wall 21 in the third direction toward the same side as the edge of the holder bottom wall 21, and circumferentially surrounds the holder bottom wall 21 to form an accommodation space open at one end.

The reagent strip 40 can be inserted into one of the accommodating cavities 25 correspondingly along the third direction. The reagent strip 40 includes a plurality of reagent tubes 41 and two limiting members 43, each reagent tube 41 is a tubular structure, the plurality of reagent tubes 41 are sequentially connected along the second direction, and the two limiting members 43 are located at two opposite ends of the reagent strip 40 in the second direction and located at one side of an opening end of the reagent tube 41. When the reagent strip 40 is inserted into the accommodating cavity 25, the two limiting members 43 are respectively limited at two side edges of the open end of the supporting frame 20 in the second direction, and the reagent tube 41 is located in the accommodating cavity 25. Thus, the reagent strip 40 is supported at the open end of the support frame 20 through the connecting member, and when the reagent strip 40 needs to be taken out, the operator can grasp the limiting member 43 to take out the reagent strip 40.

Preferably, in an embodiment, the cross section of the open end of the reagent tube 41 is rectangular, the end of the closed end of the reagent tube 41 away from the open end is conical, and the diameter of the end of the closed end of the reagent tube 41 away from the open end gradually decreases in a direction from the open end to the closed end of the reagent tube 41. It is to be understood that the shape of the reagent tube 41 is not limited thereto, and may be set as needed to meet various requirements.

Further, the edges of the two opposite sides of one end, away from the rack bottom wall 21, of the rack side wall 23 of the carrying rack 20 in the second direction are provided with fixing slots 232 having bottom walls, the plurality of fixing slots 232 on any side of the rack side wall 23 in the second direction are arranged at intervals in the first direction, and the width of each fixing slot 232 in the first direction is matched with the width of the reagent strip 40 in the first direction. The cavity walls (i.e., the inner walls of the support side walls 23) on the two opposite sides of the accommodating cavity 25 in the second direction are respectively provided with a limiting rib 234 in a protruding manner, the limiting ribs 234 on the cavity wall on each side are arranged at intervals in the first direction, each limiting rib 234 extends lengthwise along the third direction, and two sides of any one reagent strip 40 in the second direction are respectively limited between two adjacent limiting ribs 234.

In this way, the reagent strip 40 is retained on the support frame 20 by the retaining groove 232 and the retaining rib 234, and is maintained in a state of extending lengthwise in the second direction during operation.

Specifically, in one embodiment, the limiting member 43 is supported on the bottom wall of the fixing groove 232 in the third direction and protrudes out of the rack sidewall 23 in the second direction, so that the operator can directly place the reagent strip 40 on the carrier rack 20.

With reference to fig. 4 to 6, fig. 4 is a schematic structural diagram of a deep hole plate in another embodiment of the present invention; FIG. 5 shows a schematic structural view of the load bearing bracket of FIG. 4; FIG. 6 shows a schematic diagram of the structure of the reagent strip of FIG. 5.

Specifically, in another embodiment, a cross section of the limiting member 43 perpendicular to the first direction is substantially "V" shaped, the limiting member 43 is deformed in a recoverable manner in the second direction, a limiting slot 2321 is formed in a bottom wall of each fixing slot 232, and the limiting member 43 is clamped in the limiting slot 2321, so that the reagent strip 40 is stably coupled to the carrier 20.

More specifically, the limiting member 43 includes a first limiting portion 432 and a second limiting portion 434 connected to each other, one end of the first limiting portion 432 is connected to the open end of the reagent tube 41, the other end of the first limiting portion 432 extends toward the closed end of the reagent tube 41 along the third direction, one end of the second limiting portion 434 is connected to the first limiting portion 432, the other end of the second limiting portion 434 extends obliquely toward the open end of the reagent tube 41, the distance between the second limiting portion 434 and the reagent tube 41 in the second direction gradually increases, and a retaining protrusion is protruded from one end of the first limiting portion 432 connected to the second limiting portion 434 toward one side of the reagent tube 41.

Thus, the limiting member 43 is clamped in the limiting clamping groove 2321 in the first direction by the clamping protrusion, and one end of the second limiting portion 434 away from the first limiting portion 432 extends out of the limiting clamping groove 2321. When the limiting member 43 needs to be removed, the operator can rotate the second limiting portion 434 to deform the limiting member 43, so that the holding protrusion is separated from the supporting rack 20, and the reagent strip 40 can be separated from the supporting rack 20. It is understood that the shape of the limiting member 43 is not limited thereto, and the limiting member 43 and the supporting bracket 20 can be clamped together as required.

Referring again to fig. 1 and 4, in the above embodiment, the outer contour of the deep well plate 100 of the present application is substantially the same as that of the 96-well deep well plate of the prior art, so as to match with the corresponding detection instrument. Based on the size of the 96-hole deep-hole plate in the prior art, each reagent strip 40 comprises eight reagent tubes 41, and a maximum of six reagent strips 40, a maximum of forty-eight reagent tubes 41 and a maximum of forty-eight samples can be accommodated in the carrier rack 20. It is understood that the deep-well plate 100 of the present application may also be designed with different specifications and sizes as required, and during the use of the deep-well plate 100, different numbers of reagent strips 40, such as one or two reagent strips 40, may be provided as required to meet the detection requirements of different sample volumes.

In some embodiments, the carrying bracket 20 can be formed by integrally molding the mold, and can also be formed by machining, the carrying bracket 20 can be repeatedly used in different experiments, and only different reagent strips 40 need to be replaced, so that the detection cost is further reduced, and the waste of detection consumables is further reduced.

Above-mentioned deep hole board 100, can adopt different quantity's reagent strip 40 to carry out the independent assortment in order to satisfy different detection requirements, thereby higher use flexibility has, on the basis that does not change with deep hole board 100 complex instrument structure, when can realizing whole board nucleic acid and draw, single one of the share nucleic acid of compatible list again draws, also be favorable to the mistake proofing design when realizing anti-pollution better, the operation that has effectively promoted nucleic acid testing is experienced, the waste that detects the consumptive material has been reduced, it is showing and has saved the testing cost.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. A deep hole plate, characterized in that it comprises:

2. The deep-well plate according to claim 1, wherein a plurality of the reagent strips are arranged at intervals along a first direction, all the reagent tubes in each of the reagent strips are sequentially connected along a second direction, and each of the reagent tubes is inserted into the accommodating space along a third direction;

3. The deep hole plate of claim 2, wherein each reagent strip further comprises two position-limiting members, the two position-limiting members are located at two opposite ends of the reagent strip in the second direction, and the two position-limiting members are respectively positioned at two side edges of the open end of the carrying bracket in the second direction.

4. The deep hole plate of claim 3, characterised in that the stop is supported in the third direction at an edge of an open end of the carrier support.

5. The deep hole plate as claimed in claim 4, wherein the edge of the two opposite sides of the bearing bracket in the second direction is provided with a fixing groove having a bottom wall, and the position-limiting member is limited in the fixing groove.

6. The deep hole plate of claim 5, wherein the limiting member is deformable in the second direction, and a limiting slot is formed in a bottom wall of the fixing slot, and the limiting member is engaged with the limiting slot.

7. The deep hole plate as claimed in claim 4, wherein the cavity walls of the accommodating cavity on two opposite sides in the second direction are respectively provided with a plurality of limiting ribs protruding in the first direction, each limiting rib extends lengthwise along the third direction, and any one reagent strip is limited between two adjacent limiting ribs on two opposite sides in the second direction.

8. The deep well plate of any one of claims 1 to 7, wherein each reagent strip comprises eight reagent tubes, and the deep well plate comprises six reagent strips, and the six reagent strips are arranged at intervals along a straight line direction.

9. A reagent strip comprising a plurality of reagent tubes, each of the reagent tubes having an oppositely disposed closed end and an open end.

10. The reagent strip of claim 9, wherein a plurality of the reagent tubes are arranged in sequence along a straight line, and the reagent strip further comprises two limiting members, wherein the two limiting members are located at two opposite ends of the reagent strip in the arrangement direction of the reagent tubes.

11. The reagent strip of claim 9, wherein the open end of the reagent tube is rectangular in cross-section, the end of the closed end of the reagent tube distal from the open end is conical, and the diameter of the end of the closed end of the reagent tube distal from the open end decreases in a direction from the open end toward the closed end.

12. The carrying support is used for containing reagent strips and is characterized by comprising an accommodating space with one open end, and the reagent strips can be inserted into the accommodating space from the open end of the accommodating space.

13. The carrier rack of claim 12, wherein the carrier rack comprises a rack bottom wall and a rack side wall, the rack side wall extends from the edge of the rack bottom wall toward the same direction, the rack side wall circumferentially surrounds the rack bottom wall to form the accommodating space, and the edge of one end of the rack side wall away from the rack bottom wall is provided with a fixing groove having a bottom wall.

14. The load carrier as recited in claim 13, wherein the bottom wall of the retaining groove defines a retaining notch.

15. The carrying bracket according to claim 12, wherein a plurality of limiting ribs are protruded from the wall of the side wall of the bracket, the plurality of limiting ribs are arranged at intervals in a direction perpendicular to the inserting direction of the reagent strips, and the extending direction of each limiting rib is parallel to the inserting direction of the reagent strips.